Rorug05G0084100

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001644
Physical Location & Seq
Reverse (-)
7243718 .. 7245064
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0084100.1

Sequence Viewer

Length: 1347 bp
ATGGAACCACCACCGTTACTCCTCACTCTCCTCCACCTCCTCCTCCTCTCCTCCACCACCCTCTCCGCCACCACCACCCCCAACACCACTCACTCCCGCCCCCCAACTCCATCCCCCAACACCACTCACTccctccccccaaCTCCATCCCCCACTTCCTCTCCAACCCCCTCCGCTCCTTCCTCAAAAGGCTCATCTTCTCTAGACCCCAAACAAGTAAGAGCCCTCCAATCCCTCAACATCCCAACTTCCAAAGACCCATGCACTCAGCTCCACTCCAACACCACCCTCTGCGACGCCGCCAAGCCCTTCCGCCACCTCCTCTCCCTCACCCTCGCCAACTGCTCCGACGACGTCGCTCTGTCCTTCACCGCCCTCAAGTCCCTCTCCACCCTCCAATCCCTCCACTTCCTCAACTGCCCCATCGCCCCCATTCGCTTCCCCGCCGATCTCGTCGCCTCCCTCCACTCCTTCTCGGCCGTCAGCAGCCTCCGCCACCTCACCGGCGTCTGGCTCTCCCGCCTCCAGAACCTCACCGATCTAACCGTCTCCAACGTCCAAGTCAATGCCAGTGGCCCTTTCGTAATTCTCGGCAACTTGAAGAAGCTCAGGTCCGTCACTATCTCCCATGCCAACCTCACCGGTAATGTTCCCAAACACTTGAATCTCAACTTGACCCAGATCGACTTTTCCGGTAATAAGCTCGAGGGAAGAATACCCAGTTCCATTACCATACTCGAAAACCTCGAAAGCTTGAATCTTTCCTCCAATACACTCAATGGCGAAATACCCACTTCGTTTGGGGACCTCATATCCCTCAGAAATGTCTCCCTTGCTTCGAATTTGCTATCTGGGCCGATACCCGAGTCGATTTCAGCCATTCCCGGCTTGGTTCATCTTGATCTGAGCTCGAATCAGCTCAATGGCACAATCCCCAAGTTCCTTTCCGATATGAAGGGCTTGAAATTCTTGAATCTGGCCAACAATAACTTCCAGGGGGTCGTTCCTTTCAATGTGTCATTCATTAGTAAGTTGGCTTTGTTCAAGGTTAGTAGGAACAGCAATTTGTGCTACAACCATTCGGTTCTTTCGTCGAAATTGAAGCTCGGCATTGCTCCCTGTGATAAACATGGGCTGCCAATGTCGCCTCCTCCGGCGAAGGAGGACAGTTCAGCAGCGGATGACAGCAGTGATTCGGATTATGATGACAGTGATGGGGATGGCGATTCCCAAAATCAGGATCATCATCATGGGCCTAATAAGGTAGTTCTTGGTGTGGCAATTGGGCTTTCTTCCCTTGTCTTCCTCATAGTTTTCTTGATTCTTCTGTCAAAGTGGTGTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

448

Amino Acids

47.69

Weight (kDa)

7.26

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 248 - 307 1.4e-07 Leucine rich repeat
LRR_8 PF13855 272 - 331 6.1e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 357
AccBSI CCGCTC 1 cut(s) 176
AclWI GGATC 1 cut(s) 1248
AcoI YGGCCR 2 cut(s) 477, 978
AcsI RAATTY 2 cut(s) 841, 965
AcyI GRCGYC 3 cut(s) 297, 354, 507
AfiI CCNNNNNNNGG 2 cut(s) 510, 1237
AgeI ACCGGT 1 cut(s) 641
AgsI TTSAA 8 cut(s) 601, 664, 757, 964, 973, 1012, 1045, 1102
AjnI CCWGG 1 cut(s) 993
AjuI GAANNNNNNNTTGG 2 cut(s) 974, 1006
AluBI AGCT 7 cut(s) 271, 607, 703, 753, 909, 919, 1105
AluI AGCT 7 cut(s) 271, 607, 703, 753, 909, 919, 1105
Alw21I GWGCWC 1 cut(s) 911
Alw26I GTCTC 2 cut(s) 553, 832
AlwI GGATC 1 cut(s) 1248
Ama87I CYCGRG 2 cut(s) 704, 863
AoxI GGCC 5 cut(s) 477, 574, 854, 978, 1253
ApeKI GCWGC 3 cut(s) 486, 1135, 1175
ApoI RAATTY 2 cut(s) 841, 965
AsiGI ACCGGT 1 cut(s) 641
AspS9I GGNCC 5 cut(s) 575, 612, 805, 854, 1253
AsuC2I CCSGG 1 cut(s) 885
AsuHPI GGTGA 5 cut(s) 322, 361, 493, 526, 631
AsuII TTCGAA 1 cut(s) 839
AvaI CYCGRG 2 cut(s) 704, 863
AvaII GGWCC 2 cut(s) 612, 805
BaeI ACNNNNGTAYC 2 cut(s) 851, 884
BalI TGGCCA 1 cut(s) 980
BanII GRGCYC 2 cut(s) 226, 911
BbsI GAAGAC 1 cut(s) 1294
Bbv12I GWGCWC 1 cut(s) 911
BbvI GCAGC 3 cut(s) 498, 1122, 1187
BccI CCATC 5 cut(s) 118, 154, 431, 1208, 1214
BceAI ACGGC 1 cut(s) 464
BciT130I CCWGG 1 cut(s) 995
BcnI CCSGG 1 cut(s) 885
BcoDI GTCTC 2 cut(s) 553, 832
BfaI CTAG 1 cut(s) 203
BisI GCNGC 4 cut(s) 300, 487, 1136, 1176
BlsI GCNGC 4 cut(s) 301, 488, 1137, 1177
Bme1390I CCNGG 2 cut(s) 885, 995
Bme18I GGWCC 2 cut(s) 612, 805
BmeT110I CYCGRG 2 cut(s) 704, 863
BmgT120I GGNCC 5 cut(s) 575, 612, 805, 854, 1253
BmiI GGNNCC 2 cut(s) 6, 806
BmrFI CCNGG 2 cut(s) 885, 995
BmrI ACTGGG 1 cut(s) 714
BmuI ACTGGG 1 cut(s) 714
BpiI GAAGAC 1 cut(s) 1294
BpmI CTGGAG 1 cut(s) 509
Bpu10I CCTNAGC 1 cut(s) 608
Bpu14I TTCGAA 1 cut(s) 839
BpuEI CTTGAG 1 cut(s) 362
BpuMI CCSGG 1 cut(s) 885
BsaBI GATNNNNATC 1 cut(s) 1245
BsaHI GRCGYC 3 cut(s) 297, 354, 507
BsaJI CCNNGG 1 cut(s) 994
BsaWI WCCGGW 2 cut(s) 641, 692
BsaXI ACNNNNNCTCC 5 cut(s) 33, 145, 175, 308, 338
Bsc4I CCNNNNNNNGG 2 cut(s) 510, 1237
Bse118I RCCGGY 2 cut(s) 503, 641
Bse1I ACTGG 2 cut(s) 570, 720
Bse3DI GCAATG 1 cut(s) 1110
Bse8I GATNNNNATC 1 cut(s) 1245
BseBI CCWGG 1 cut(s) 995
BseDI CCNNGG 1 cut(s) 994
BseGI GGATG 5 cut(s) 110, 146, 240, 1186, 1225
BseJI GATNNNNATC 1 cut(s) 1245
BseLI CCNNNNNNNGG 2 cut(s) 510, 1237
BseMI GCAATG 1 cut(s) 1110
BseMII CTCAG 4 cut(s) 281, 622, 832, 896
BseNI ACTGG 2 cut(s) 570, 720
BseRI GAGGAG 8 cut(s) 11, 20, 29, 32, 35, 40, 311, 1140
BseX3I CGGCCG 1 cut(s) 477
BseXI GCAGC 3 cut(s) 498, 1122, 1187
Bsh1285I CGRYCG 1 cut(s) 480
BshFI GGCC 5 cut(s) 479, 576, 856, 980, 1255
BshTI ACCGGT 1 cut(s) 641
BsiEI CGRYCG 1 cut(s) 480
BsiHKAI GWGCWC 1 cut(s) 911
BsiHKCI CYCGRG 2 cut(s) 704, 863
BsiSI CCGG 5 cut(s) 504, 642, 693, 885, 1154
BslFI GGGAC 2 cut(s) 367, 818
BslI CCNNNNNNNGG 2 cut(s) 510, 1237
BsmAI GTCTC 2 cut(s) 553, 832
BsmBI CGTCTC 1 cut(s) 553
BsmFI GGGAC 2 cut(s) 367, 818
BsnI GGCC 5 cut(s) 479, 576, 856, 980, 1255
BsoBI CYCGRG 2 cut(s) 704, 863
Bsp119I TTCGAA 1 cut(s) 839
Bsp1286I GDGCHC 2 cut(s) 226, 911
Bsp143I GATC 5 cut(s) 448, 538, 681, 901, 1240
BspANI GGCC 5 cut(s) 479, 576, 856, 980, 1255
BspCNI CTCAG 4 cut(s) 280, 621, 831, 897
BspLI GGNNCC 2 cut(s) 6, 806
BspPI GGATC 1 cut(s) 1248
BspT104I TTCGAA 1 cut(s) 839
BsrBI CCGCTC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 1110
BsrFI RCCGGY 2 cut(s) 503, 641
BsrI ACTGG 2 cut(s) 570, 720
BssAI RCCGGY 2 cut(s) 503, 641
BssECI CCNNGG 1 cut(s) 994
BssMI GATC 5 cut(s) 448, 538, 681, 901, 1240
BssNI GRCGYC 3 cut(s) 297, 354, 507
Bst2UI CCWGG 1 cut(s) 995
Bst4CI ACNGT 4 cut(s) 15, 547, 1169, 1211
BstACI GRCGYC 3 cut(s) 297, 354, 507
BstAPI GCANNNNNTGC 1 cut(s) 1068
BstBI TTCGAA 1 cut(s) 839
BstDEI CTNAG 4 cut(s) 267, 608, 818, 905
BstF5I GGATG 5 cut(s) 110, 146, 240, 1186, 1225
BstKTI GATC 5 cut(s) 451, 541, 684, 904, 1243
BstMAI GTCTC 2 cut(s) 553, 832
BstMBI GATC 5 cut(s) 448, 538, 681, 901, 1240
BstMCI CGRYCG 1 cut(s) 480
BstMWI GCNNNNNNNGC 4 cut(s) 492, 853, 1068, 1144
BstNI CCWGG 1 cut(s) 995
BstSCI CCNGG 2 cut(s) 883, 993
BstV1I GCAGC 3 cut(s) 498, 1122, 1187
BstV2I GAAGAC 1 cut(s) 1294
BstZI CGGCCG 1 cut(s) 477
BsuRI GGCC 5 cut(s) 479, 576, 856, 980, 1255
BtgZI GCGATG 1 cut(s) 409
BtsCI GGATG 5 cut(s) 110, 146, 240, 1186, 1225
BtsI GCAGTG 1 cut(s) 1195
BtsIMutI CAGTG 3 cut(s) 577, 1195, 1216
Cfr10I RCCGGY 2 cut(s) 503, 641
Cfr13I GGNCC 5 cut(s) 575, 612, 805, 854, 1253
CseI GACGC 2 cut(s) 305, 496
CspAI ACCGGT 1 cut(s) 641
CspCI CAANNNNNGTGG 4 cut(s) 553, 588, 781, 816
CviAII CATG 4 cut(s) 261, 629, 1130, 1250
DdeI CTNAG 4 cut(s) 267, 608, 818, 905
DpnI GATC 5 cut(s) 450, 540, 683, 903, 1242
DpnII GATC 5 cut(s) 448, 538, 681, 901, 1240
EaeI YGGCCR 2 cut(s) 477, 978
EagI CGGCCG 1 cut(s) 477
EciI GGCGGA 3 cut(s) 55, 302, 482
Ecl136II GAGCTC 1 cut(s) 909
EclXI CGGCCG 1 cut(s) 477
Eco24I GRGCYC 2 cut(s) 226, 911
Eco47I GGWCC 2 cut(s) 612, 805
Eco52I CGGCCG 1 cut(s) 477
Eco53kI GAGCTC 1 cut(s) 909
Eco88I CYCGRG 2 cut(s) 704, 863
EcoICRI GAGCTC 1 cut(s) 909
EcoO109I RGGNCCY 1 cut(s) 805
EcoRII CCWGG 1 cut(s) 993
EcoT38I GRGCYC 2 cut(s) 226, 911
Esp3I CGTCTC 1 cut(s) 553
FaeI CATG 4 cut(s) 264, 632, 1133, 1253
FaiI YATR 9 cut(s) 262, 630, 734, 812, 953, 1131, 1203, 1251, 1310
FaqI GGGAC 2 cut(s) 367, 818
FatI CATG 4 cut(s) 260, 628, 1129, 1249
FauI CCCGC 3 cut(s) 104, 451, 527
Fnu4HI GCNGC 4 cut(s) 300, 487, 1136, 1176
FokI GGATG 5 cut(s) 97, 133, 227, 1193, 1232
FriOI GRGCYC 2 cut(s) 226, 911
Fsp4HI GCNGC 4 cut(s) 300, 487, 1136, 1176
FspBI CTAG 1 cut(s) 203
GluI GCNGC 4 cut(s) 300, 487, 1136, 1176
GsuI CTGGAG 1 cut(s) 509
HaeIII GGCC 5 cut(s) 479, 576, 856, 980, 1255
HapII CCGG 5 cut(s) 504, 642, 693, 885, 1154
HgaI GACGC 2 cut(s) 305, 496
Hin1I GRCGYC 3 cut(s) 297, 354, 507
Hin1II CATG 4 cut(s) 264, 632, 1133, 1253
HindIII AAGCTT 1 cut(s) 751
HinfI GANTC 8 cut(s) 664, 757, 866, 913, 973, 1193, 1226, 1321
HpaII CCGG 5 cut(s) 504, 642, 693, 885, 1154
HphI GGTGA 5 cut(s) 322, 361, 493, 526, 631
Hpy188I TCNGA 5 cut(s) 349, 821, 906, 949, 1198
Hpy188III TCNNGA 6 cut(s) 203, 526, 899, 970, 1238, 1318
Hpy99I CGWCG 6 cut(s) 299, 353, 356, 359, 458, 1096
HpyAV CCTTC 6 cut(s) 189, 319, 376, 481, 949, 1153
HpyCH4III ACNGT 4 cut(s) 15, 547, 1169, 1211
HpyCH4IV ACGT 2 cut(s) 354, 555
HpyCH4V TGCA 1 cut(s) 264
HpyF10VI GCNNNNNNNGC 4 cut(s) 492, 853, 1068, 1144
HpyF3I CTNAG 4 cut(s) 267, 608, 818, 905
HpySE526I ACGT 2 cut(s) 354, 555
Hsp92I GRCGYC 3 cut(s) 297, 354, 507
Hsp92II CATG 4 cut(s) 264, 632, 1133, 1253
Kzo9I GATC 5 cut(s) 448, 538, 681, 901, 1240
LmnI GCTCC 4 cut(s) 181, 276, 350, 1120
Lsp1109I GCAGC 3 cut(s) 498, 1122, 1187
MaeI CTAG 1 cut(s) 203
MaeII ACGT 2 cut(s) 354, 555
MaeIII GTNAC 2 cut(s) 15, 616
MalI GATC 5 cut(s) 450, 540, 683, 903, 1242
MbiI CCGCTC 1 cut(s) 176
MboI GATC 5 cut(s) 448, 538, 681, 901, 1240
MboII GAAGA 6 cut(s) 189, 613, 723, 1284, 1294, 1316
MfeI CAATTG 1 cut(s) 1281
MhlI GDGCHC 2 cut(s) 226, 911
MlsI TGGCCA 1 cut(s) 980
MluCI AATT 6 cut(s) 585, 841, 965, 1063, 1097, 1281
MluNI TGGCCA 1 cut(s) 980
MlyI GAGTC 1 cut(s) 875
MmeI TCCRAC 4 cut(s) 188, 303, 372, 576
Mox20I TGGCCA 1 cut(s) 980
MscI TGGCCA 1 cut(s) 980
MslI CAYNNNNRTG 1 cut(s) 1248
Msp20I TGGCCA 1 cut(s) 980
MspA1I CMGCKG 1 cut(s) 1178
MspI CCGG 5 cut(s) 504, 642, 693, 885, 1154
MspR9I CCNGG 2 cut(s) 885, 995
MunI CAATTG 1 cut(s) 1281
MvaI CCWGG 1 cut(s) 995
MwoI GCNNNNNNNGC 4 cut(s) 492, 853, 1068, 1144
NciI CCSGG 1 cut(s) 885
NdeII GATC 5 cut(s) 448, 538, 681, 901, 1240
NlaIII CATG 4 cut(s) 264, 632, 1133, 1253
NlaIV GGNNCC 2 cut(s) 6, 806
NmeAIII GCCGAG 3 cut(s) 455, 570, 1086
NmuCI GTSAC 1 cut(s) 616
NspV TTCGAA 1 cut(s) 839
PaeR7I CTCGAG 1 cut(s) 704
PcsI WCGNNNNNNNCGW 2 cut(s) 744, 1088
PfeI GAWTC 7 cut(s) 664, 757, 913, 973, 1193, 1226, 1321
PflFI GACNNNGTC 1 cut(s) 353
PinAI ACCGGT 1 cut(s) 641
PkrI GCNGC 4 cut(s) 301, 488, 1137, 1177
PleI GAGTC 1 cut(s) 874
PpsI GAGTC 1 cut(s) 874
PpuMI RGGWCCY 1 cut(s) 805
Psp124BI GAGCTC 1 cut(s) 911
Psp5II RGGWCCY 1 cut(s) 805
Psp6I CCWGG 1 cut(s) 993
PspGI CCWGG 1 cut(s) 993
PspN4I GGNNCC 2 cut(s) 6, 806
PspPI GGNCC 5 cut(s) 575, 612, 805, 854, 1253
PspPPI RGGWCCY 1 cut(s) 805
PspXI VCTCGAGB 1 cut(s) 704
PsyI GACNNNGTC 1 cut(s) 353
RseI CAYNNNNRTG 1 cut(s) 1248
SacI GAGCTC 1 cut(s) 911
SatI GCNGC 4 cut(s) 300, 487, 1136, 1176
Sau3AI GATC 5 cut(s) 448, 538, 681, 901, 1240
Sau96I GGNCC 5 cut(s) 575, 612, 805, 854, 1253
SchI GAGTC 1 cut(s) 875
ScrFI CCNGG 2 cut(s) 885, 995
SduI GDGCHC 2 cut(s) 226, 911
Sfr274I CTCGAG 1 cut(s) 704
SfuI TTCGAA 1 cut(s) 839
SgrAI CRCCGGYG 1 cut(s) 503
SinI GGWCC 2 cut(s) 612, 805
SlaI CTCGAG 1 cut(s) 704
SmiMI CAYNNNNRTG 1 cut(s) 1248
SmlI CTYRAG 2 cut(s) 377, 704
SmoI CTYRAG 2 cut(s) 377, 704
Sse9I AATT 6 cut(s) 585, 841, 965, 1063, 1097, 1281
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 1 cut(s) 911
StyD4I CCNGG 2 cut(s) 883, 993
TaaI ACNGT 4 cut(s) 15, 547, 1169, 1211
TaiI ACGT 2 cut(s) 357, 558
TaqI TCGA 8 cut(s) 684, 705, 738, 747, 839, 869, 911, 1094
TasI AATT 6 cut(s) 585, 841, 965, 1063, 1097, 1281
TauI GCSGC 1 cut(s) 302
TfiI GAWTC 7 cut(s) 664, 757, 913, 973, 1193, 1226, 1321
TscAI CASTG 3 cut(s) 577, 1195, 1216
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 3 cut(s) 486, 1135, 1175
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 3 cut(s) 884, 968, 1012
TspGWI ACGGA 1 cut(s) 604
TspRI CASTG 3 cut(s) 577, 1195, 1216
Tth111I GACNNNGTC 1 cut(s) 353
VpaK11BI GGWCC 2 cut(s) 612, 805
XapI RAATTY 2 cut(s) 841, 965
XbaI TCTAGA 1 cut(s) 202
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XhoI CTCGAG 1 cut(s) 704
XspI CTAG 1 cut(s) 203
ZraI GACGTC 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.