Rorug05G0084200

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001644
Physical Location & Seq
Reverse (-)
7248996 .. 7254374
5379 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0084200.1

Sequence Viewer

Length: 2934 bp
ATGATGCAATCTGAGGATCATCATCATCATCTATGCAGTACCTGTGGTGATGCCAATGGGGAAGTGTTTGTGGCTTGCCATGAATGCAATTCCCCTATTTGCAGGGCCTGTTTTGATGAGGATGTCAAGGAGGGGCGCAAAGTTTGCTTGCAGTGTGGCACTGCCTATGATGAGAAGTCACTGGCTGAGCACGAAACAAAGGTATCCGGCACCCGATCCACAATGGCATCTCACCTGGATAATGCTACGGATGTTGGAATTCATGCTAGACATGTCAGCACTGTGTCTACAGTGGATAGTGAATATAATGATGAATCTGGGAATCCAATATGGAAGAATCGAGTAGAGAGTTGGAAGGATAAAAAGAGCAAGAAGAAAAAGGATGCGGCTAAGGCAGAAAAGGTGGCTGAAATTCCACCTGAGCAGCAAATGGCAGATAATCTTGCCCCAGATGCTGCGCAACCACTTTCAACTGTCGTTCCACTTCCATCTAGTAAAATCACACCATACAGGATTATAATAATTACACGACTGATCATTCTTGGTCTCTTCTTCCATTATCGAGTCACAAATCCTGTTGATAGTGCTTATGGTCTATGGCTCACTTCTATCATATGTGAGATCTGGTTTGCTTTTTCTTGGGTGTTGGATCAGTTTCCTAAGTGGTCTCCAGTTAGTAGGGTTACATATATTGACACTTTATCGGCCAGATTTGAAAGAGAGGGTGAGCCTTCTCAGCTAGCTCCTGTGGATTTCTTCGTGAGTACAGTCGATCCATTGAAAGAACCACCCTTGATCACTGCCAATACTGTGCTTTCCATTCTTGCTATTGACTATCCTGTGGAGAAAGTTTCCTGCTATGTGTCTGATGACGGTGCTGCCATGCTTAGTTTCGAATCTCTTGTAGAAACATCTGAATTTGCAAGAAAGTGGGTTCCATTCTGCAAAAAATTCTCGATTGAACCACGTGCACCTGAGTTCTACTTCTCACAGAAGATTGACTACTTGAAGGATAAAGTGCAACCCTCTTTTGTGAAGGAGCGCAGAGCAATGAAAAGAGACTATGAGGAGTTCAAAGTGAGAATGAATGCTTTGGTAGCAAAGGCTCAAAAGACACCAGAAGATGGCTGGACTATGCAAGATGGAACACCTTGGCCAGGAAATAACTCACGTGACCATCCTGGCATGATCCAGGTTTTTCTTGGACATAGCGGCGCCCTTGACATTGAGGGAAATGAGCTTCCTCGATTGGTTTATGTCTCCAGAGAGAAGAGACCTGGGTACCAACACCACAAGAAAGCTGGTGCTGAAAATGCTCTGGTAAGGGTGTCTGCAGTTCTCACAAATGCCCCATTCATCCTCAATCTCGACTGTGATCACTACGTTAACAACAGCAAGGCAATTCGTGAGGCAATGTGCTTCTTGATGGATCCACAAGTTGGTAGAGATCTATGTTATGTGCAGTTTCCTCAGAGATTTGATGGCATTGATCGAAGTGACAGATATGCCAATCGCAATACAGTTTTCTTTGATGTTAACATGAAAGGACTGGATGGCATTCAAGGACCGGTATATGTGGGGACAGGTTGTTGTTTCAATAGGCAGGCACTTTATGGCTATAGTCCTTCTATGCCAAGCTTATCCAAGGCATCGTCCTCATCCTGTTCTTGGTGTGGCTGCTTCTCTTGTTGCTGCCCCTCCAAGAAGCCAACTAAAGATCTGTCAGAGGCTTTCCGAGATGCAAAACGAGAGGAGCTTGATGCTGCCATTTTTAACCTCAGGGAGATTGAAAACTACGATGAGTATGAGAGGTCAATGCTGATGTCCCAGACCAGCTTTGAGAAAACTTTTGGCTTATCAACTGTATTTATTGAGTCTACACTAATGGAGTATGGAGGAGTCGCTGAATCTTCCAACCCATCACTATTAATCAAGGAGGCAATTCATGTCATTAGCTGTGGTTATGAAGAGAAGACCGCATGGGGAAAAGAGATTGGTTGGATTTATGGATCAGTCACTGAGGATATCTTAACCGGTTTCAAGATGCACTGCCGTGGATGGAGATCAGTTTACTGCATGCCCTTGAGGCCTGCATTCAAAGGTTCAGCTCCCATTAATCTTTCTGATCGTCTGCACCAAGTCCTCAGGTGGGCACTTGGATCTGTGGAAATCTTCCTCAGTAGACATTGTCCTCTCTGGTACGGGTTTGCAGGAGGCCGCCTCAAGTGGCTTCAGCGCCTGGCATACATTAACACCATTGTTTACCCCTTCACATCCCTCCCTCTCATCGCTTACTGCTCGCTGCCTGCAATATGCCTTCTCACAGGAAAATTCATCATCCCAACACTTTCGAATCTGGCAAGTGTTTTGTTTCTTGGCCTCTTCCTCTCCATCATTCTCACTAGTGTCATTGAGTTGCGGTGGAGTGGTGTCAGCATCCAAGACTTGTGGCGTAATGAGCAGTTCTGGGTCATAGGAGGTGTTTCAGCCCATCTCTTTGCTGTCTTCCAAGGTTTCCTCAAGATGTTGGCTGGTGTGGACACCAACTTCACTGTCACATCTAAAGCGGCCGAAGATGCAGACTTCGGAGAGCTCTATATGATCAAATGGACTACACTTCTGATTCCCCCAACTACACTCCTCATTGTGAACATGGTTGGTGTTGTTGCTGGATTTTCTGATGCACTAAACAAGGGTTATGAAGCTTGGGGGCCACTATTTGGCAAGGTTTTCTTTGCCTTCTGGGTGATTCTTCATCTCTATCCATTCCTCAAAGGTCTCATGGGACGCCAAAACCGGACTCCAACCATTGTTGTTTTGTGGTCAATTCTGTTGGCCTCTGTCTTCTCTCTTGTTTGGGTCAGGATAAATCCATTTGTGAGCAAAGTCGATAACTCAGCCCTTACTACAGACTGCATTTCCATCGACTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

977

Amino Acids

109.84

Weight (kDa)

6.35

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-UDP PF14569 6 - 58 3.1e-12 Zinc-binding RING-finger
Cellulose_synt PF03552 250 - 968 0.0 Cellulose synthase
Glyco_trans_2_3 PF13632 664 - 767 5.2e-06 Glycosyl transferase family group 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 518
Acc16I TGCGCA 1 cut(s) 459
Acc65I GGTACC 1 cut(s) 1281
AccB1I GGYRCC 3 cut(s) 209, 1214, 1281
AccB7I CCANNNNNTGG 3 cut(s) 1124, 1439, 2720
AccI GTMKAC 3 cut(s) 287, 1877, 2182
AciI CCGC 6 cut(s) 386, 1212, 1977, 2218, 2419, 2567
AclWI GGATC 9 cut(s) 24, 210, 657, 767, 1183, 1424, 1437, 2017, 2167
AcoI YGGCCR 3 cut(s) 705, 1154, 2568
AcsI RAATTY 5 cut(s) 258, 411, 917, 950, 2330
AcuI CTGAAG 1 cut(s) 2216
AcvI CACGTG 2 cut(s) 968, 1172
AcyI GRCGYC 2 cut(s) 1215, 2788
AfaI GTAC 4 cut(s) 40, 766, 1283, 2201
AfiI CCNNNNNNNGG 6 cut(s) 1124, 1157, 1439, 1668, 2149, 2720
AflIII ACRYGT 1 cut(s) 271
AgeI ACCGGT 2 cut(s) 1567, 2033
AhlI ACTAGT 1 cut(s) 2402
AjnI CCWGG 6 cut(s) 234, 1156, 1180, 1191, 1276, 2238
AleI CACNNNNGTG 1 cut(s) 2052
Alw21I GWGCWC 3 cut(s) 192, 973, 2595
Alw26I GTCTC 6 cut(s) 551, 672, 1053, 1264, 1267, 2783
Alw44I GTGCAC 1 cut(s) 969
AlwI GGATC 9 cut(s) 24, 210, 657, 767, 1183, 1424, 1437, 2017, 2167
AlwNI CAGNNNCTG 5 cut(s) 42, 108, 455, 2018, 2239
AoxI GGCC 9 cut(s) 105, 705, 1154, 2087, 2215, 2377, 2568, 2711, 2835
ApaLI GTGCAC 1 cut(s) 969
ApeKI GCWGC 7 cut(s) 424, 455, 878, 1677, 1692, 1763, 2302
ApoI RAATTY 5 cut(s) 258, 411, 917, 950, 2330
AseI ATTAAT 2 cut(s) 1928, 2115
AsiGI ACCGGT 2 cut(s) 1567, 2033
Asp718I GGTACC 1 cut(s) 1281
AspLEI GCGC 5 cut(s) 138, 460, 1044, 1217, 2238
AspS9I GGNCC 3 cut(s) 105, 1565, 2711
AsuHPI GGTGA 4 cut(s) 59, 224, 737, 2758
AsuII TTCGAA 2 cut(s) 894, 2351
AsuNHI GCTAGC 1 cut(s) 739
AvaII GGWCC 1 cut(s) 1565
AxyI CCTNAGG 2 cut(s) 1778, 2144
BaeGI GKGCMC 2 cut(s) 973, 2155
BaeI ACNNNNGTAYC 4 cut(s) 186, 219, 1265, 1298
BalI TGGCCA 1 cut(s) 1156
BamHI GGATCC 1 cut(s) 1429
BanI GGYRCC 3 cut(s) 209, 1214, 1281
BanII GRGCYC 1 cut(s) 2595
BarI GAAGNNNNNNTAC 2 cut(s) 986, 1018
BbrPI CACGTG 2 cut(s) 968, 1172
BbsI GAAGAC 3 cut(s) 1979, 2497, 2836
Bbv12I GWGCWC 3 cut(s) 192, 973, 2595
BbvI GCAGC 7 cut(s) 436, 442, 865, 1664, 1679, 1750, 2289
BceAI ACGGC 1 cut(s) 2037
BcgI CGANNNNNNTGC 1 cut(s) 2905
BciT130I CCWGG 6 cut(s) 236, 1158, 1182, 1193, 1278, 2240
BciVI GTATCC 1 cut(s) 214
BclI TGATCA 4 cut(s) 534, 795, 1375, 2601
BcoDI GTCTC 6 cut(s) 551, 672, 1053, 1264, 1267, 2783
BcuI ACTAGT 1 cut(s) 2402
BfaI CTAG 4 cut(s) 267, 492, 740, 2403
BfmI CTRYAG 4 cut(s) 288, 1332, 1618, 2907
BfoI RGCGCY 2 cut(s) 1218, 2239
BfuI GTATCC 1 cut(s) 214
BglI GCCNNNNNGGC 1 cut(s) 2086
BglII AGATCT 3 cut(s) 621, 1447, 1717
BlpI GCTNAGC 1 cut(s) 186
Bme1390I CCNGG 6 cut(s) 236, 1158, 1182, 1193, 1278, 2240
Bme18I GGWCC 1 cut(s) 1565
BmgT120I GGNCC 3 cut(s) 105, 1565, 2711
BmiI GGNNCC 6 cut(s) 211, 936, 1216, 1283, 1431, 2712
BmrFI CCNGG 6 cut(s) 236, 1158, 1182, 1193, 1278, 2240
BmtI GCTAGC 1 cut(s) 743
BpiI GAAGAC 3 cut(s) 1979, 2497, 2836
BplI GAGNNNNNCTC 2 cut(s) 2205, 2237
BpmI CTGGAG 2 cut(s) 654, 1246
Bpu10I CCTNAGC 2 cut(s) 390, 420
Bpu1102I GCTNAGC 1 cut(s) 186
Bpu14I TTCGAA 2 cut(s) 894, 2351
BpuEI CTTGAG 3 cut(s) 2104, 2207, 2504
BsaAI YACGTR 2 cut(s) 968, 1172
BsaBI GATNNNNATC 2 cut(s) 21, 2062
BsaHI GRCGYC 2 cut(s) 1215, 2788
BsaI GGTCTC 4 cut(s) 551, 672, 1267, 2783
BsaJI CCNNGG 5 cut(s) 1151, 1277, 1644, 2053, 2509
BsaWI WCCGGW 3 cut(s) 1567, 2033, 2796
Bsc4I CCNNNNNNNGG 6 cut(s) 1124, 1157, 1439, 1668, 2149, 2720
Bse118I RCCGGY 2 cut(s) 1567, 2033
Bse1I ACTGG 3 cut(s) 186, 671, 1554
Bse21I CCTNAGG 2 cut(s) 1778, 2144
Bse3DI GCAATG 2 cut(s) 1056, 1419
Bse8I GATNNNNATC 2 cut(s) 21, 2062
BseBI CCWGG 6 cut(s) 236, 1158, 1182, 1193, 1278, 2240
BseDI CCNNGG 5 cut(s) 1151, 1277, 1644, 2053, 2509
BseJI GATNNNNATC 2 cut(s) 21, 2062
BseLI CCNNNNNNNGG 6 cut(s) 1124, 1157, 1439, 1668, 2149, 2720
BseMI GCAATG 2 cut(s) 1056, 1419
BseNI ACTGG 3 cut(s) 186, 671, 1554
BseRI GAGGAG 4 cut(s) 1082, 1766, 1911, 2630
BseSI GKGCMC 2 cut(s) 973, 2155
BseX3I CGGCCG 1 cut(s) 2568
BseXI GCAGC 7 cut(s) 436, 442, 865, 1664, 1679, 1750, 2289
BsgI GTGCAG 2 cut(s) 1481, 2117
Bsh1285I CGRYCG 1 cut(s) 2571
BshFI GGCC 9 cut(s) 107, 707, 1156, 2089, 2217, 2379, 2570, 2713, 2837
BshNI GGYRCC 3 cut(s) 209, 1214, 1281
BshTI ACCGGT 2 cut(s) 1567, 2033
BsiEI CGRYCG 1 cut(s) 2571
BsiHKAI GWGCWC 3 cut(s) 192, 973, 2595
BsiSI CCGG 4 cut(s) 207, 1568, 2034, 2797
BslFI GGGAC 3 cut(s) 1594, 1810, 2799
BslI CCNNNNNNNGG 6 cut(s) 1124, 1157, 1439, 1668, 2149, 2720
BsmAI GTCTC 6 cut(s) 551, 672, 1053, 1264, 1267, 2783
BsmFI GGGAC 3 cut(s) 1594, 1810, 2799
BsmI GAATGC 4 cut(s) 89, 1093, 1557, 2093
BsnI GGCC 9 cut(s) 107, 707, 1156, 2089, 2217, 2379, 2570, 2713, 2837
Bso31I GGTCTC 4 cut(s) 551, 672, 1267, 2783
Bsp119I TTCGAA 2 cut(s) 894, 2351
Bsp1286I GDGCHC 4 cut(s) 192, 973, 2155, 2595
Bsp1720I GCTNAGC 1 cut(s) 186
BspACI CCGC 6 cut(s) 386, 1212, 1977, 2218, 2419, 2567
BspANI GGCC 9 cut(s) 107, 707, 1156, 2089, 2217, 2379, 2570, 2713, 2837
BspLI GGNNCC 6 cut(s) 211, 936, 1216, 1283, 1431, 2712
BspMAI CTGCAG 1 cut(s) 1336
BspOI GCTAGC 1 cut(s) 743
BspPI GGATC 9 cut(s) 24, 210, 657, 767, 1183, 1424, 1437, 2017, 2167
BspT104I TTCGAA 2 cut(s) 894, 2351
BspT107I GGYRCC 3 cut(s) 209, 1214, 1281
BspTNI GGTCTC 4 cut(s) 551, 672, 1267, 2783
BsrDI GCAATG 2 cut(s) 1056, 1419
BsrFI RCCGGY 2 cut(s) 1567, 2033
BsrI ACTGG 3 cut(s) 186, 671, 1554
BssAI RCCGGY 2 cut(s) 1567, 2033
BssECI CCNNGG 5 cut(s) 1151, 1277, 1644, 2053, 2509
BssNI GRCGYC 2 cut(s) 1215, 2788
BssT1I CCWWGG 3 cut(s) 1151, 1644, 2509
Bst2UI CCWGG 6 cut(s) 236, 1158, 1182, 1193, 1278, 2240
Bst6I CTCTTC 4 cut(s) 554, 1265, 1962, 2387
BstACI GRCGYC 2 cut(s) 1215, 2788
BstAPI GCANNNNNTGC 1 cut(s) 144
BstBAI YACGTR 2 cut(s) 968, 1172
BstBI TTCGAA 2 cut(s) 894, 2351
BstC8I GCNNGC 8 cut(s) 76, 149, 741, 1605, 2078, 2091, 2300, 2307
BstDSI CCRYGG 1 cut(s) 2053
BstENI CCTNNNNNAGG 1 cut(s) 1155
BstH2I RGCGCY 2 cut(s) 1218, 2239
BstHHI GCGC 5 cut(s) 138, 460, 1044, 1217, 2238
BstMAI GTCTC 6 cut(s) 551, 672, 1053, 1264, 1267, 2783
BstMCI CGRYCG 1 cut(s) 2571
BstNI CCWGG 6 cut(s) 236, 1158, 1182, 1193, 1278, 2240
BstNSI RCATGY 2 cut(s) 275, 2080
BstSCI CCNGG 6 cut(s) 234, 1156, 1180, 1191, 1276, 2238
BstSFI CTRYAG 4 cut(s) 288, 1332, 1618, 2907
BstSLI GKGCMC 2 cut(s) 973, 2155
BstV1I GCAGC 7 cut(s) 436, 442, 865, 1664, 1679, 1750, 2289
BstV2I GAAGAC 3 cut(s) 1979, 2497, 2836
BstX2I RGATCY 5 cut(s) 621, 1429, 1447, 1717, 2159
BstYI RGATCY 5 cut(s) 621, 1429, 1447, 1717, 2159
BstZI CGGCCG 1 cut(s) 2568
Bsu36I CCTNAGG 2 cut(s) 1778, 2144
BsuI GTATCC 1 cut(s) 214
BsuRI GGCC 9 cut(s) 107, 707, 1156, 2089, 2217, 2379, 2570, 2713, 2837
BtgI CCRYGG 1 cut(s) 2053
BtgZI GCGATG 1 cut(s) 2272
BtsI GCAGTG 4 cut(s) 158, 159, 798, 2047
BtsIMutI CAGTG 9 cut(s) 158, 159, 179, 279, 297, 798, 2016, 2047, 2550
Cac8I GCNNGC 8 cut(s) 76, 149, 741, 1605, 2078, 2091, 2300, 2307
CaiI CAGNNNCTG 5 cut(s) 42, 108, 455, 2018, 2239
CfoI GCGC 5 cut(s) 138, 460, 1044, 1217, 2238
Cfr10I RCCGGY 2 cut(s) 1567, 2033
Cfr13I GGNCC 3 cut(s) 105, 1565, 2711
CseI GACGC 1 cut(s) 2796
Csp6I GTAC 4 cut(s) 39, 765, 1282, 2200
CspAI ACCGGT 2 cut(s) 1567, 2033
CspCI CAANNNNNGTGG 2 cut(s) 2429, 2464
CviQI GTAC 4 cut(s) 39, 765, 1282, 2200
DinI GGCGCC 1 cut(s) 1216
EaeI YGGCCR 3 cut(s) 705, 1154, 2568
EagI CGGCCG 1 cut(s) 2568
Eam1104I CTCTTC 4 cut(s) 554, 1265, 1962, 2387
EarI CTCTTC 4 cut(s) 554, 1265, 1962, 2387
Ecl136II GAGCTC 1 cut(s) 2593
EclXI CGGCCG 1 cut(s) 2568
Eco130I CCWWGG 3 cut(s) 1151, 1644, 2509
Eco147I AGGCCT 1 cut(s) 2089
Eco24I GRGCYC 1 cut(s) 2595
Eco31I GGTCTC 4 cut(s) 551, 672, 1267, 2783
Eco32I GATATC 1 cut(s) 2026
Eco47I GGWCC 1 cut(s) 1565
Eco52I CGGCCG 1 cut(s) 2568
Eco53kI GAGCTC 1 cut(s) 2593
Eco57I CTGAAG 1 cut(s) 2216
Eco72I CACGTG 2 cut(s) 968, 1172
Eco81I CCTNAGG 2 cut(s) 1778, 2144
EcoICRI GAGCTC 1 cut(s) 2593
EcoNI CCTNNNNNAGG 1 cut(s) 1155
EcoO109I RGGNCCY 1 cut(s) 105
EcoRI GAATTC 1 cut(s) 258
EcoRII CCWGG 6 cut(s) 234, 1156, 1180, 1191, 1276, 2238
EcoRV GATATC 1 cut(s) 2026
EcoT14I CCWWGG 3 cut(s) 1151, 1644, 2509
EcoT38I GRGCYC 1 cut(s) 2595
EgeI GGCGCC 1 cut(s) 1216
EheI GGCGCC 1 cut(s) 1216
ErhI CCWWGG 3 cut(s) 1151, 1644, 2509
FalI AAGNNNNNCTT 2 cut(s) 2717, 2749
FaqI GGGAC 3 cut(s) 1594, 1810, 2799
FauNDI CATATG 1 cut(s) 614
FbaI TGATCA 4 cut(s) 534, 795, 1375, 2601
FblI GTMKAC 3 cut(s) 287, 1877, 2182
FriOI GRGCYC 1 cut(s) 2595
FspBI CTAG 4 cut(s) 267, 492, 740, 2403
FspI TGCGCA 1 cut(s) 459
GlaI GCGC 5 cut(s) 137, 459, 1043, 1216, 2237
GsuI CTGGAG 2 cut(s) 654, 1246
HaeII RGCGCY 2 cut(s) 1218, 2239
HaeIII GGCC 9 cut(s) 107, 707, 1156, 2089, 2217, 2379, 2570, 2713, 2837
HapII CCGG 4 cut(s) 207, 1568, 2034, 2797
HgaI GACGC 1 cut(s) 2796
HhaI GCGC 5 cut(s) 138, 460, 1044, 1217, 2238
Hin1I GRCGYC 2 cut(s) 1215, 2788
Hin6I GCGC 5 cut(s) 136, 458, 1042, 1215, 2236
HinP1I GCGC 5 cut(s) 136, 458, 1042, 1215, 2236
HincII GTYRAC 2 cut(s) 1387, 1537
HindII GTYRAC 2 cut(s) 1387, 1537
HindIII AAGCTT 2 cut(s) 1636, 2703
HpaI GTTAAC 2 cut(s) 1387, 1537
HpaII CCGG 4 cut(s) 207, 1568, 2034, 2797
HphI GGTGA 4 cut(s) 59, 224, 737, 2758
Hpy188III TCNNGA 9 cut(s) 760, 955, 1263, 1367, 1406, 1423, 2041, 2521, 2863
HpyAV CCTTC 8 cut(s) 349, 741, 1003, 1030, 1635, 2278, 2327, 2749
HpyCH4IV ACGT 3 cut(s) 967, 1171, 1383
HpySE526I ACGT 3 cut(s) 967, 1171, 1383
Hsp92I GRCGYC 2 cut(s) 1215, 2788
HspAI GCGC 5 cut(s) 136, 458, 1042, 1215, 2236
KasI GGCGCC 1 cut(s) 1214
KpnI GGTACC 1 cut(s) 1285
Ksp22I TGATCA 4 cut(s) 534, 795, 1375, 2601
KspAI GTTAAC 2 cut(s) 1387, 1537
LmnI GCTCC 4 cut(s) 748, 1039, 1753, 2113
Lsp1109I GCAGC 7 cut(s) 436, 442, 865, 1664, 1679, 1750, 2289
MaeI CTAG 4 cut(s) 267, 492, 740, 2403
MaeII ACGT 3 cut(s) 967, 1171, 1383
MaeIII GTNAC 7 cut(s) 177, 565, 682, 1172, 1496, 2014, 2554
MflI RGATCY 5 cut(s) 621, 1429, 1447, 1717, 2159
MhlI GDGCHC 4 cut(s) 192, 973, 2155, 2595
MlsI TGGCCA 1 cut(s) 1156
MluNI TGGCCA 1 cut(s) 1156
Mly113I GGCGCC 1 cut(s) 1215
MlyI GAGTC 4 cut(s) 573, 1883, 1908, 2794
MmeI TCCRAC 6 cut(s) 235, 332, 627, 1938, 1979, 2828
Mox20I TGGCCA 1 cut(s) 1156
MscI TGGCCA 1 cut(s) 1156
MseI TTAA 8 cut(s) 1386, 1536, 1773, 1928, 2030, 2115, 2250, 2932
MslI CAYNNNNRTG 1 cut(s) 2052
Msp20I TGGCCA 1 cut(s) 1156
MspI CCGG 4 cut(s) 207, 1568, 2034, 2797
MspR9I CCNGG 6 cut(s) 236, 1158, 1182, 1193, 1278, 2240
Mva1269I GAATGC 4 cut(s) 89, 1093, 1557, 2093
MvaI CCWGG 6 cut(s) 236, 1158, 1182, 1193, 1278, 2240
NarI GGCGCC 1 cut(s) 1215
NdeI CATATG 1 cut(s) 614
NheI GCTAGC 1 cut(s) 739
NlaIV GGNNCC 6 cut(s) 211, 936, 1216, 1283, 1431, 2712
NmuCI GTSAC 6 cut(s) 177, 565, 1172, 1496, 2014, 2554
NsbI TGCGCA 1 cut(s) 459
NspI RCATGY 2 cut(s) 275, 2080
NspV TTCGAA 2 cut(s) 894, 2351
OliI CACNNNNGTG 1 cut(s) 2052
PaeI GCATGC 1 cut(s) 2080
PceI AGGCCT 1 cut(s) 2089
PciI ACATGT 1 cut(s) 271
PctI GAATGC 4 cut(s) 89, 1093, 1557, 2093
PfeI GAWTC 8 cut(s) 314, 322, 337, 896, 1907, 2353, 2623, 2749
PflFI GACNNNGTC 1 cut(s) 2187
PflMI CCANNNNNTGG 3 cut(s) 1124, 1439, 2720
PinAI ACCGGT 2 cut(s) 1567, 2033
PleI GAGTC 4 cut(s) 572, 1882, 1907, 2794
PluTI GGCGCC 1 cut(s) 1218
PmaCI CACGTG 2 cut(s) 968, 1172
PmlI CACGTG 2 cut(s) 968, 1172
PpsI GAGTC 4 cut(s) 572, 1882, 1907, 2794
Ppu21I YACGTR 2 cut(s) 968, 1172
PscI ACATGT 1 cut(s) 271
PshBI ATTAAT 2 cut(s) 1928, 2115
PsiI TTATAA 1 cut(s) 518
Psp124BI GAGCTC 1 cut(s) 2595
Psp6I CCWGG 6 cut(s) 234, 1156, 1180, 1191, 1276, 2238
PspCI CACGTG 2 cut(s) 968, 1172
PspGI CCWGG 6 cut(s) 234, 1156, 1180, 1191, 1276, 2238
PspN4I GGNNCC 6 cut(s) 211, 936, 1216, 1283, 1431, 2712
PspPI GGNCC 3 cut(s) 105, 1565, 2711
PstI CTGCAG 1 cut(s) 1336
PstNI CAGNNNCTG 5 cut(s) 42, 108, 455, 2018, 2239
PsuI RGATCY 5 cut(s) 621, 1429, 1447, 1717, 2159
PsyI GACNNNGTC 1 cut(s) 2187
RsaI GTAC 4 cut(s) 40, 766, 1283, 2201
RsaNI GTAC 4 cut(s) 39, 765, 1282, 2200
RseI CAYNNNNRTG 1 cut(s) 2052
SacI GAGCTC 1 cut(s) 2595
SaqAI TTAA 8 cut(s) 1386, 1536, 1773, 1928, 2030, 2115, 2250, 2932
Sau96I GGNCC 3 cut(s) 105, 1565, 2711
SchI GAGTC 4 cut(s) 573, 1883, 1908, 2794
ScrFI CCNGG 6 cut(s) 236, 1158, 1182, 1193, 1278, 2240
SduI GDGCHC 4 cut(s) 192, 973, 2155, 2595
SfcI CTRYAG 4 cut(s) 288, 1332, 1618, 2907
SfoI GGCGCC 1 cut(s) 1216
SfuI TTCGAA 2 cut(s) 894, 2351
SinI GGWCC 1 cut(s) 1565
SmiMI CAYNNNNRTG 1 cut(s) 2052
SmlI CTYRAG 3 cut(s) 2083, 2222, 2519
SmoI CTYRAG 3 cut(s) 2083, 2222, 2519
SpeI ACTAGT 1 cut(s) 2402
SphI GCATGC 1 cut(s) 2080
SseBI AGGCCT 1 cut(s) 2089
SsiI CCGC 6 cut(s) 386, 1212, 1977, 2218, 2419, 2567
SspDI GGCGCC 1 cut(s) 1214
SspMI CTAG 4 cut(s) 267, 492, 740, 2403
SstI GAGCTC 1 cut(s) 2595
StuI AGGCCT 1 cut(s) 2089
StyD4I CCNGG 6 cut(s) 234, 1156, 1180, 1191, 1276, 2238
StyI CCWWGG 3 cut(s) 1151, 1644, 2509
TaiI ACGT 3 cut(s) 970, 1174, 1386
TatI WGTACW 1 cut(s) 764
TauI GCSGC 4 cut(s) 389, 1215, 2220, 2570
TfiI GAWTC 8 cut(s) 314, 322, 337, 896, 1907, 2353, 2623, 2749
Tru1I TTAA 8 cut(s) 1386, 1536, 1773, 1928, 2030, 2115, 2250, 2932
Tru9I TTAA 8 cut(s) 1386, 1536, 1773, 1928, 2030, 2115, 2250, 2932
TscAI CASTG 9 cut(s) 158, 166, 186, 286, 297, 805, 2023, 2054, 2557
TseFI GTSAC 6 cut(s) 177, 565, 1172, 1496, 2014, 2554
TseI GCWGC 7 cut(s) 424, 455, 878, 1677, 1692, 1763, 2302
Tsp45I GTSAC 6 cut(s) 177, 565, 1172, 1496, 2014, 2554
TspGWI ACGGA 1 cut(s) 263
TspRI CASTG 9 cut(s) 158, 166, 186, 286, 297, 805, 2023, 2054, 2557
Tth111I GACNNNGTC 1 cut(s) 2187
Van91I CCANNNNNTGG 3 cut(s) 1124, 1439, 2720
VneI GTGCAC 1 cut(s) 969
VpaK11BI GGWCC 1 cut(s) 1565
VspI ATTAAT 2 cut(s) 1928, 2115
XagI CCTNNNNNAGG 1 cut(s) 1155
XapI RAATTY 5 cut(s) 258, 411, 917, 950, 2330
XceI RCATGY 2 cut(s) 275, 2080
XcmI CCANNNNNNNNNTGG 3 cut(s) 1125, 1199, 1298
XmiI GTMKAC 3 cut(s) 287, 1877, 2182
XspI CTAG 4 cut(s) 267, 492, 740, 2403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.