CRAL/TRIO, N-terminal domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2099 |
| AarI | CACCTGC | 1 cut(s) | 2230 |
| AatII | GACGTC | 1 cut(s) | 2409 |
| Acc36I | ACCTGC | 1 cut(s) | 2230 |
| AccI | GTMKAC | 1 cut(s) | 2539 |
| AccII | CGCG | 1 cut(s) | 2684 |
| AciI | CCGC | 3 cut(s) | 161, 821, 1445 |
| AclWI | GGATC | 6 cut(s) | 44, 1515, 2097, 2468, 2650, 2684 |
| AcsI | RAATTY | 5 cut(s) | 16, 1847, 2372, 2621, 2778 |
| AcuI | CTGAAG | 3 cut(s) | 75, 2097, 2308 |
| AcyI | GRCGYC | 1 cut(s) | 2406 |
| AfaI | GTAC | 8 cut(s) | 289, 922, 1132, 1797, 2067, 2315, 2420, 2661 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1911, 2464, 2635 |
| AflIII | ACRYGT | 1 cut(s) | 2091 |
| AjnI | CCWGG | 2 cut(s) | 2152, 2200 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1424, 1456, 2357, 2389 |
| Alw26I | GTCTC | 2 cut(s) | 1601, 2506 |
| AlwI | GGATC | 6 cut(s) | 44, 1515, 2097, 2468, 2650, 2684 |
| AoxI | GGCC | 7 cut(s) | 270, 340, 550, 972, 1182, 2387, 2455 |
| ApeKI | GCWGC | 6 cut(s) | 113, 158, 1691, 2035, 2330, 2552 |
| ApoI | RAATTY | 5 cut(s) | 16, 1847, 2372, 2621, 2778 |
| ArsI | GACNNNNNNTTYG | 8 cut(s) | 1937, 1969, 1998, 2030, 2188, 2220, 2484, 2516 |
| AspLEI | GCGC | 2 cut(s) | 2684, 2686 |
| AspS9I | GGNCC | 6 cut(s) | 340, 550, 760, 973, 1183, 2204 |
| AsuC2I | CCSGG | 2 cut(s) | 2012, 2727 |
| AsuHPI | GGTGA | 7 cut(s) | 385, 595, 805, 1018, 1228, 1970, 2334 |
| AsuII | TTCGAA | 1 cut(s) | 2224 |
| AvaII | GGWCC | 2 cut(s) | 760, 2204 |
| BanII | GRGCYC | 1 cut(s) | 2727 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2306, 2338 |
| BauI | CACGAG | 1 cut(s) | 2815 |
| BbvI | GCAGC | 6 cut(s) | 100, 170, 1678, 2022, 2317, 2564 |
| BccI | CCATC | 1 cut(s) | 2215 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2659, 2693 |
| BciT130I | CCWGG | 2 cut(s) | 2154, 2202 |
| BclI | TGATCA | 5 cut(s) | 445, 655, 865, 1078, 2178 |
| BcnI | CCSGG | 2 cut(s) | 2012, 2727 |
| BcoDI | GTCTC | 2 cut(s) | 1601, 2506 |
| BfaI | CTAG | 3 cut(s) | 1809, 2487, 2588 |
| BfuAI | ACCTGC | 1 cut(s) | 2230 |
| BglII | AGATCT | 1 cut(s) | 2508 |
| BisI | GCNGC | 6 cut(s) | 114, 159, 1692, 2036, 2331, 2553 |
| BlsI | GCNGC | 6 cut(s) | 115, 160, 1693, 2037, 2332, 2554 |
| Bme1390I | CCNGG | 4 cut(s) | 2012, 2154, 2202, 2727 |
| Bme18I | GGWCC | 2 cut(s) | 760, 2204 |
| BmgT120I | GGNCC | 6 cut(s) | 340, 550, 760, 973, 1183, 2204 |
| BmiI | GGNNCC | 3 cut(s) | 405, 825, 2724 |
| BmrFI | CCNGG | 4 cut(s) | 2012, 2154, 2202, 2727 |
| BmrI | ACTGGG | 1 cut(s) | 2078 |
| BmsI | GCATC | 7 cut(s) | 91, 265, 475, 685, 898, 1108, 1870 |
| BmuI | ACTGGG | 1 cut(s) | 2078 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1860 |
| Bpu14I | TTCGAA | 1 cut(s) | 2224 |
| BpuEI | CTTGAG | 2 cut(s) | 1873, 2399 |
| BpuMI | CCSGG | 2 cut(s) | 2012, 2727 |
| Bsa29I | ATCGAT | 1 cut(s) | 1932 |
| BsaBI | GATNNNNATC | 2 cut(s) | 2190, 2513 |
| BsaHI | GRCGYC | 1 cut(s) | 2406 |
| BsaJI | CCNNGG | 8 cut(s) | 312, 407, 522, 732, 945, 1040, 1155, 1253 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1911, 2464, 2635 |
| Bse1I | ACTGG | 2 cut(s) | 2073, 2441 |
| Bse8I | GATNNNNATC | 2 cut(s) | 2190, 2513 |
| BseBI | CCWGG | 2 cut(s) | 2154, 2202 |
| BseCI | ATCGAT | 1 cut(s) | 1932 |
| BseDI | CCNNGG | 8 cut(s) | 312, 407, 522, 732, 945, 1040, 1155, 1253 |
| BseGI | GGATG | 9 cut(s) | 97, 308, 361, 518, 728, 941, 1151, 1276, 1664 |
| BseJI | GATNNNNATC | 2 cut(s) | 2190, 2513 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1911, 2464, 2635 |
| BseMII | CTCAG | 6 cut(s) | 44, 234, 1131, 1442, 2473, 2517 |
| BseNI | ACTGG | 2 cut(s) | 2073, 2441 |
| BsePI | GCGCGC | 1 cut(s) | 2682 |
| BseXI | GCAGC | 6 cut(s) | 100, 170, 1678, 2022, 2317, 2564 |
| Bsh1236I | CGCG | 1 cut(s) | 2684 |
| BshFI | GGCC | 7 cut(s) | 272, 342, 552, 974, 1184, 2389, 2457 |
| BshVI | ATCGAT | 1 cut(s) | 1932 |
| BsiSI | CCGG | 2 cut(s) | 2012, 2727 |
| BslFI | GGGAC | 1 cut(s) | 2019 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1911, 2464, 2635 |
| BsmAI | GTCTC | 2 cut(s) | 1601, 2506 |
| BsmFI | GGGAC | 1 cut(s) | 2019 |
| BsnI | GGCC | 7 cut(s) | 272, 342, 552, 974, 1184, 2389, 2457 |
| Bsp119I | TTCGAA | 1 cut(s) | 2224 |
| Bsp1286I | GDGCHC | 1 cut(s) | 2727 |
| Bsp1407I | TGTACA | 1 cut(s) | 2659 |
| Bsp19I | CCATGG | 5 cut(s) | 312, 522, 732, 945, 1155 |
| BspACI | CCGC | 3 cut(s) | 161, 821, 1445 |
| BspANI | GGCC | 7 cut(s) | 272, 342, 552, 974, 1184, 2389, 2457 |
| BspCNI | CTCAG | 6 cut(s) | 43, 235, 1132, 1443, 2472, 2516 |
| BspDI | ATCGAT | 1 cut(s) | 1932 |
| BspFNI | CGCG | 1 cut(s) | 2684 |
| BspHI | TCATGA | 1 cut(s) | 2175 |
| BspLI | GGNNCC | 3 cut(s) | 405, 825, 2724 |
| BspMI | ACCTGC | 1 cut(s) | 2230 |
| BspPI | GGATC | 6 cut(s) | 44, 1515, 2097, 2468, 2650, 2684 |
| BspQI | GCTCTTC | 2 cut(s) | 1308, 2256 |
| BspT104I | TTCGAA | 1 cut(s) | 2224 |
| BsrGI | TGTACA | 1 cut(s) | 2659 |
| BsrI | ACTGG | 2 cut(s) | 2073, 2441 |
| BssECI | CCNNGG | 8 cut(s) | 312, 407, 522, 732, 945, 1040, 1155, 1253 |
| BssHII | GCGCGC | 1 cut(s) | 2682 |
| BssNAI | GTATAC | 1 cut(s) | 2540 |
| BssNI | GRCGYC | 1 cut(s) | 2406 |
| BssSI | CACGAG | 1 cut(s) | 2815 |
| BssT1I | CCWWGG | 8 cut(s) | 312, 407, 522, 732, 945, 1040, 1155, 1253 |
| Bst1107I | GTATAC | 1 cut(s) | 2540 |
| Bst2BI | CACGAG | 1 cut(s) | 2815 |
| Bst2UI | CCWGG | 2 cut(s) | 2154, 2202 |
| BstACI | GRCGYC | 1 cut(s) | 2406 |
| BstAUI | TGTACA | 1 cut(s) | 2659 |
| BstBI | TTCGAA | 1 cut(s) | 2224 |
| BstC8I | GCNNGC | 1 cut(s) | 2684 |
| BstDSI | CCRYGG | 5 cut(s) | 312, 522, 732, 945, 1155 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2462 |
| BstF5I | GGATG | 9 cut(s) | 97, 308, 361, 518, 728, 941, 1151, 1276, 1664 |
| BstFNI | CGCG | 1 cut(s) | 2684 |
| BstHHI | GCGC | 2 cut(s) | 2684, 2686 |
| BstMAI | GTCTC | 2 cut(s) | 1601, 2506 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 110, 2327, 2558 |
| BstNI | CCWGG | 2 cut(s) | 2154, 2202 |
| BstNSI | RCATGY | 1 cut(s) | 2095 |
| BstSCI | CCNGG | 4 cut(s) | 2010, 2152, 2200, 2725 |
| BstUI | CGCG | 1 cut(s) | 2684 |
| BstV1I | GCAGC | 6 cut(s) | 100, 170, 1678, 2022, 2317, 2564 |
| BstX2I | RGATCY | 2 cut(s) | 2102, 2508 |
| BstYI | RGATCY | 2 cut(s) | 2102, 2508 |
| BstZ17I | GTATAC | 1 cut(s) | 2540 |
| Bsu15I | ATCGAT | 1 cut(s) | 1932 |
| BsuRI | GGCC | 7 cut(s) | 272, 342, 552, 974, 1184, 2389, 2457 |
| BsuTUI | ATCGAT | 1 cut(s) | 1932 |
| BtgI | CCRYGG | 5 cut(s) | 312, 522, 732, 945, 1155 |
| BtgZI | GCGATG | 3 cut(s) | 708, 921, 1131 |
| BtsCI | GGATG | 9 cut(s) | 97, 308, 361, 518, 728, 941, 1151, 1276, 1664 |
| BtsIMutI | CAGTG | 1 cut(s) | 2332 |
| BveI | ACCTGC | 1 cut(s) | 2230 |
| Cac8I | GCNNGC | 1 cut(s) | 2684 |
| CciI | TCATGA | 1 cut(s) | 2175 |
| CfoI | GCGC | 2 cut(s) | 2684, 2686 |
| Cfr13I | GGNCC | 6 cut(s) | 340, 550, 760, 973, 1183, 2204 |
| ClaI | ATCGAT | 1 cut(s) | 1932 |
| Csp6I | GTAC | 8 cut(s) | 288, 921, 1131, 1796, 2066, 2314, 2419, 2660 |
| CviAII | CATG | 9 cut(s) | 313, 523, 733, 946, 1156, 1427, 1478, 2092, 2176 |
| CviQI | GTAC | 8 cut(s) | 288, 921, 1131, 1796, 2066, 2314, 2419, 2660 |
| DraI | TTTAAA | 1 cut(s) | 21 |
| EciI | GGCGGA | 1 cut(s) | 1460 |
| Eco130I | CCWWGG | 8 cut(s) | 312, 407, 522, 732, 945, 1040, 1155, 1253 |
| Eco24I | GRGCYC | 1 cut(s) | 2727 |
| Eco47I | GGWCC | 2 cut(s) | 760, 2204 |
| Eco57I | CTGAAG | 3 cut(s) | 75, 2097, 2308 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2462 |
| EcoRII | CCWGG | 2 cut(s) | 2152, 2200 |
| EcoT14I | CCWWGG | 8 cut(s) | 312, 407, 522, 732, 945, 1040, 1155, 1253 |
| EcoT38I | GRGCYC | 1 cut(s) | 2727 |
| ErhI | CCWWGG | 8 cut(s) | 312, 407, 522, 732, 945, 1040, 1155, 1253 |
| FaeI | CATG | 9 cut(s) | 316, 526, 736, 949, 1159, 1430, 1481, 2095, 2179 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2129, 2161 |
| FaqI | GGGAC | 1 cut(s) | 2019 |
| FatI | CATG | 9 cut(s) | 312, 522, 732, 945, 1155, 1426, 1477, 2091, 2175 |
| FauNDI | CATATG | 1 cut(s) | 2647 |
| FbaI | TGATCA | 5 cut(s) | 445, 655, 865, 1078, 2178 |
| FblI | GTMKAC | 1 cut(s) | 2539 |
| Fnu4HI | GCNGC | 6 cut(s) | 114, 159, 1692, 2036, 2331, 2553 |
| FokI | GGATG | 9 cut(s) | 104, 295, 368, 505, 715, 928, 1138, 1283, 1651 |
| FriOI | GRGCYC | 1 cut(s) | 2727 |
| Fsp4HI | GCNGC | 6 cut(s) | 114, 159, 1692, 2036, 2331, 2553 |
| FspBI | CTAG | 3 cut(s) | 1809, 2487, 2588 |
| GlaI | GCGC | 2 cut(s) | 2683, 2685 |
| GluI | GCNGC | 6 cut(s) | 114, 159, 1692, 2036, 2331, 2553 |
| HaeIII | GGCC | 7 cut(s) | 272, 342, 552, 974, 1184, 2389, 2457 |
| HapII | CCGG | 2 cut(s) | 2012, 2727 |
| HhaI | GCGC | 2 cut(s) | 2684, 2686 |
| Hin1I | GRCGYC | 1 cut(s) | 2406 |
| Hin1II | CATG | 9 cut(s) | 316, 526, 736, 949, 1159, 1430, 1481, 2095, 2179 |
| Hin6I | GCGC | 2 cut(s) | 2682, 2684 |
| HinP1I | GCGC | 2 cut(s) | 2682, 2684 |
| HincII | GTYRAC | 1 cut(s) | 2742 |
| HindII | GTYRAC | 1 cut(s) | 2742 |
| HinfI | GANTC | 4 cut(s) | 860, 1358, 1934, 2397 |
| HpaI | GTTAAC | 1 cut(s) | 2742 |
| HpaII | CCGG | 2 cut(s) | 2012, 2727 |
| HphI | GGTGA | 7 cut(s) | 385, 595, 805, 1018, 1228, 1970, 2334 |
| Hpy166II | GTNNAC | 4 cut(s) | 1876, 2540, 2608, 2742 |
| Hpy188I | TCNGA | 9 cut(s) | 33, 865, 1357, 1414, 2136, 2462, 2506, 2533, 2702 |
| Hpy8I | GTNNAC | 4 cut(s) | 1876, 2540, 2608, 2742 |
| HpyCH4IV | ACGT | 1 cut(s) | 2406 |
| HpyCH4V | TGCA | 7 cut(s) | 83, 104, 498, 708, 1694, 1784, 2282 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 110, 2327, 2558 |
| HpySE526I | ACGT | 1 cut(s) | 2406 |
| Hsp92I | GRCGYC | 1 cut(s) | 2406 |
| Hsp92II | CATG | 9 cut(s) | 316, 526, 736, 949, 1159, 1430, 1481, 2095, 2179 |
| HspAI | GCGC | 2 cut(s) | 2682, 2684 |
| Ksp22I | TGATCA | 5 cut(s) | 445, 655, 865, 1078, 2178 |
| KspAI | GTTAAC | 1 cut(s) | 2742 |
| LguI | GCTCTTC | 2 cut(s) | 1308, 2256 |
| LmnI | GCTCC | 5 cut(s) | 1447, 2218, 2667, 2722, 2756 |
| Lsp1109I | GCAGC | 6 cut(s) | 100, 170, 1678, 2022, 2317, 2564 |
| LweI | GCATC | 7 cut(s) | 91, 265, 475, 685, 898, 1108, 1870 |
| MaeI | CTAG | 3 cut(s) | 1809, 2487, 2588 |
| MaeII | ACGT | 1 cut(s) | 2406 |
| MaeIII | GTNAC | 3 cut(s) | 2018, 2087, 2783 |
| MflI | RGATCY | 2 cut(s) | 2102, 2508 |
| MhlI | GDGCHC | 1 cut(s) | 2727 |
| MlyI | GAGTC | 1 cut(s) | 2406 |
| MmeI | TCCRAC | 3 cut(s) | 1155, 1318, 1692 |
| MseI | TTAA | 7 cut(s) | 20, 1388, 1571, 1895, 2031, 2148, 2741 |
| MspA1I | CMGCKG | 2 cut(s) | 161, 1736 |
| MspI | CCGG | 2 cut(s) | 2012, 2727 |
| MspR9I | CCNGG | 4 cut(s) | 2012, 2154, 2202, 2727 |
| MvaI | CCWGG | 2 cut(s) | 2154, 2202 |
| MvnI | CGCG | 1 cut(s) | 2684 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 110, 2327, 2558 |
| NciI | CCSGG | 2 cut(s) | 2012, 2727 |
| NcoI | CCATGG | 5 cut(s) | 312, 522, 732, 945, 1155 |
| NdeI | CATATG | 1 cut(s) | 2647 |
| NlaIII | CATG | 9 cut(s) | 316, 526, 736, 949, 1159, 1430, 1481, 2095, 2179 |
| NlaIV | GGNNCC | 3 cut(s) | 405, 825, 2724 |
| NmeAIII | GCCGAG | 1 cut(s) | 2365 |
| NspI | RCATGY | 1 cut(s) | 2095 |
| NspV | TTCGAA | 1 cut(s) | 2224 |
| PagI | TCATGA | 1 cut(s) | 2175 |
| PaqCI | CACCTGC | 1 cut(s) | 2230 |
| PauI | GCGCGC | 1 cut(s) | 2682 |
| PciI | ACATGT | 1 cut(s) | 2091 |
| PciSI | GCTCTTC | 2 cut(s) | 1308, 2256 |
| PfeI | GAWTC | 3 cut(s) | 860, 1358, 1934 |
| PkrI | GCNGC | 6 cut(s) | 115, 160, 1693, 2037, 2332, 2554 |
| PleI | GAGTC | 1 cut(s) | 2405 |
| PpsI | GAGTC | 1 cut(s) | 2405 |
| PscI | ACATGT | 1 cut(s) | 2091 |
| PsiI | TTATAA | 1 cut(s) | 2099 |
| Psp6I | CCWGG | 2 cut(s) | 2152, 2200 |
| PspGI | CCWGG | 2 cut(s) | 2152, 2200 |
| PspN4I | GGNNCC | 3 cut(s) | 405, 825, 2724 |
| PspPI | GGNCC | 6 cut(s) | 340, 550, 760, 973, 1183, 2204 |
| PsuI | RGATCY | 2 cut(s) | 2102, 2508 |
| PteI | GCGCGC | 1 cut(s) | 2682 |
| PvuII | CAGCTG | 1 cut(s) | 1736 |
| RsaI | GTAC | 8 cut(s) | 289, 922, 1132, 1797, 2067, 2315, 2420, 2661 |
| RsaNI | GTAC | 8 cut(s) | 288, 921, 1131, 1796, 2066, 2314, 2419, 2660 |
| SapI | GCTCTTC | 2 cut(s) | 1308, 2256 |
| SaqAI | TTAA | 7 cut(s) | 20, 1388, 1571, 1895, 2031, 2148, 2741 |
| SatI | GCNGC | 6 cut(s) | 114, 159, 1692, 2036, 2331, 2553 |
| Sau96I | GGNCC | 6 cut(s) | 340, 550, 760, 973, 1183, 2204 |
| SchI | GAGTC | 1 cut(s) | 2406 |
| ScrFI | CCNGG | 4 cut(s) | 2012, 2154, 2202, 2727 |
| SduI | GDGCHC | 1 cut(s) | 2727 |
| SfaNI | GCATC | 7 cut(s) | 91, 265, 475, 685, 898, 1108, 1870 |
| SfuI | TTCGAA | 1 cut(s) | 2224 |
| SinI | GGWCC | 2 cut(s) | 760, 2204 |
| SmlI | CTYRAG | 2 cut(s) | 1852, 2414 |
| SmoI | CTYRAG | 2 cut(s) | 1852, 2414 |
| SsiI | CCGC | 3 cut(s) | 161, 821, 1445 |
| SspI | AATATT | 1 cut(s) | 2172 |
| SspMI | CTAG | 3 cut(s) | 1809, 2487, 2588 |
| StyD4I | CCNGG | 4 cut(s) | 2010, 2152, 2200, 2725 |
| StyI | CCWWGG | 8 cut(s) | 312, 407, 522, 732, 945, 1040, 1155, 1253 |
| TaiI | ACGT | 1 cut(s) | 2409 |
| TaqI | TCGA | 6 cut(s) | 633, 1932, 1937, 2142, 2224, 2748 |
| TatI | WGTACW | 2 cut(s) | 2418, 2659 |
| TfiI | GAWTC | 3 cut(s) | 860, 1358, 1934 |
| Tru1I | TTAA | 7 cut(s) | 20, 1388, 1571, 1895, 2031, 2148, 2741 |
| Tru9I | TTAA | 7 cut(s) | 20, 1388, 1571, 1895, 2031, 2148, 2741 |
| TscAI | CASTG | 1 cut(s) | 2332 |
| TseI | GCWGC | 6 cut(s) | 113, 158, 1691, 2035, 2330, 2552 |
| TspGWI | ACGGA | 1 cut(s) | 416 |
| TspRI | CASTG | 1 cut(s) | 2332 |
| VpaK11BI | GGWCC | 2 cut(s) | 760, 2204 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2462 |
| XapI | RAATTY | 5 cut(s) | 16, 1847, 2372, 2621, 2778 |
| XceI | RCATGY | 1 cut(s) | 2095 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 205 |
| XmiI | GTMKAC | 1 cut(s) | 2539 |
| XspI | CTAG | 3 cut(s) | 1809, 2487, 2588 |
| ZraI | GACGTC | 1 cut(s) | 2407 |