Rmu_co8308705.1_g000001

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8308705.1
OG0001644
Physical Location & Seq
Reverse (-)
1 .. 914
914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8308705.1_g000001.1.cds

Sequence Viewer

Length: 797 bp
cagaggaaacagagcctgctgttaaagcagaggaaacagaggctgttgtgaaaacagagtgtgctgaaaaaacagaggaaggagaaaaggcaccagaattggctgagaagaaagaagaagtagcagttccggtatgcgaggaagaagcgaaacccgaatccagcgaagtcgtcgacacagatatctcactctggggtgtgcctctgttccctggaaaaggatctgagggaactgatgtggtgctcttgaaattcttgagggccagggagttcaaggtgaacgaagccttcgagatgttgaagaagactctgcagtggaggaaggagtcgaaaattgattcaattttgggtgaggagatctgctctgatctgagctctgctgcgtacttgaacggcgtggatcgcgaaggccaccctgtttgctacaacatcttcggagtctttgacaacgaagagctttaccagaagacgttcggaaatgaagagaagagaggccaattcttgaggtggagggttcaattgatggagaagagtatccagaagcttgaattaaggcctggtggtgctacttctctgcttcagatcaatgacctcaagaactcgcctgggccaatcaagaaggagcttagggttgcaacaaagcaagctgttgggctcttgcaggacaattaccctgaattggttgccaaaaatattttcatcaatgttccattctggtactatgcactcaatgctctgttgtctccattcttgactcaaagaaccaagagcaagtttgtggtggctcgccccgctaag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

265

Amino Acids

29.9

Weight (kDa)

5.12

Isoelectric Point (pI)

56.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 90
AccI GTMKAC 1 cut(s) 173
AccII CGCG 1 cut(s) 404
AciI CCGC 1 cut(s) 791
AclWI GGATC 2 cut(s) 228, 407
AcsI RAATTY 1 cut(s) 250
AcuI CTGAAG 1 cut(s) 562
AfaI GTAC 2 cut(s) 385, 718
AfiI CCNNNNNNNGG 2 cut(s) 217, 678
AgsI TTSAA 7 cut(s) 249, 273, 300, 341, 390, 517, 547
AjnI CCWGG 4 cut(s) 210, 262, 555, 603
AjuI GAANNNNNNNTTGG 2 cut(s) 679, 711
AluBI AGCT 5 cut(s) 374, 456, 543, 624, 646
AluI AGCT 5 cut(s) 374, 456, 543, 624, 646
Alw21I GWGCWC 2 cut(s) 245, 376
Alw26I GTCTC 1 cut(s) 746
AlwI GGATC 2 cut(s) 228, 407
AlwNI CAGNNNCTG 2 cut(s) 16, 43
AoxI GGCC 5 cut(s) 260, 408, 492, 553, 607
ApeKI GCWGC 1 cut(s) 379
ApoI RAATTY 1 cut(s) 250
Asp700I GAANNNNTTC 1 cut(s) 469
AspS9I GGNCC 2 cut(s) 260, 607
AsuHPI GGTGA 2 cut(s) 288, 361
BanI GGYRCC 1 cut(s) 90
BanII GRGCYC 2 cut(s) 376, 656
BbsI GAAGAC 2 cut(s) 310, 472
Bbv12I GWGCWC 2 cut(s) 245, 376
BbvI GCAGC 1 cut(s) 366
BccI CCATC 1 cut(s) 516
BceAI ACGGC 1 cut(s) 408
BciT130I CCWGG 4 cut(s) 212, 264, 557, 605
BciVI GTATCC 1 cut(s) 544
BcoDI GTCTC 1 cut(s) 746
BfmI CTRYAG 1 cut(s) 310
BfuI GTATCC 1 cut(s) 544
BglII AGATCT 1 cut(s) 356
BisI GCNGC 1 cut(s) 380
BlsI GCNGC 1 cut(s) 381
Bme1390I CCNGG 4 cut(s) 212, 264, 557, 605
BmgT120I GGNCC 2 cut(s) 260, 607
BmiI GGNNCC 1 cut(s) 92
BmrFI CCNGG 4 cut(s) 212, 264, 557, 605
BpiI GAAGAC 2 cut(s) 310, 472
BplI GAGNNNNNCTC 2 cut(s) 346, 378
Bpu10I CCTNAGC 1 cut(s) 625
BpuEI CTTGAG 3 cut(s) 276, 522, 577
BsaJI CCNNGG 3 cut(s) 210, 263, 604
BsaWI WCCGGW 1 cut(s) 129
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 678
BseBI CCWGG 4 cut(s) 212, 264, 557, 605
BseDI CCNNGG 3 cut(s) 210, 263, 604
BseLI CCNNNNNNNGG 2 cut(s) 217, 678
BseMII CTCAG 3 cut(s) 95, 215, 361
BseRI GAGGAG 1 cut(s) 367
BseXI GCAGC 1 cut(s) 366
Bsh1236I CGCG 1 cut(s) 404
BshFI GGCC 5 cut(s) 262, 410, 494, 555, 609
BshNI GGYRCC 1 cut(s) 90
BsiHKAI GWGCWC 2 cut(s) 245, 376
BsiSI CCGG 1 cut(s) 130
BslI CCNNNNNNNGG 2 cut(s) 217, 678
BsmAI GTCTC 1 cut(s) 746
BsnI GGCC 5 cut(s) 262, 410, 494, 555, 609
Bsp1286I GDGCHC 3 cut(s) 245, 376, 656
Bsp143I GATC 5 cut(s) 220, 356, 366, 399, 581
Bsp68I TCGCGA 1 cut(s) 404
BspACI CCGC 1 cut(s) 791
BspANI GGCC 5 cut(s) 262, 410, 494, 555, 609
BspCNI CTCAG 3 cut(s) 96, 216, 362
BspFNI CGCG 1 cut(s) 404
BspLI GGNNCC 1 cut(s) 92
BspMAI CTGCAG 1 cut(s) 314
BspPI GGATC 2 cut(s) 228, 407
BspQI GCTCTTC 1 cut(s) 446
BspT107I GGYRCC 1 cut(s) 90
BssECI CCNNGG 3 cut(s) 210, 263, 604
BssMI GATC 5 cut(s) 220, 356, 366, 399, 581
Bst2UI CCWGG 4 cut(s) 212, 264, 557, 605
Bst6I CTCTTC 4 cut(s) 446, 476, 481, 523
BstAPI GCANNNNNTGC 1 cut(s) 730
BstC8I GCNNGC 3 cut(s) 17, 644, 786
BstDEI CTNAG 5 cut(s) 104, 224, 370, 625, 794
BstENI CCTNNNNNAGG 1 cut(s) 215
BstFNI CGCG 1 cut(s) 404
BstKTI GATC 5 cut(s) 223, 359, 369, 402, 584
BstMAI GTCTC 1 cut(s) 746
BstMBI GATC 5 cut(s) 220, 356, 366, 399, 581
BstMWI GCNNNNNNNGC 4 cut(s) 25, 401, 730, 790
BstNI CCWGG 4 cut(s) 212, 264, 557, 605
BstSCI CCNGG 4 cut(s) 210, 262, 555, 603
BstSFI CTRYAG 1 cut(s) 310
BstUI CGCG 1 cut(s) 404
BstV1I GCAGC 1 cut(s) 366
BstV2I GAAGAC 2 cut(s) 310, 472
BstX2I RGATCY 2 cut(s) 220, 356
BstYI RGATCY 2 cut(s) 220, 356
BsuI GTATCC 1 cut(s) 544
BsuRI GGCC 5 cut(s) 262, 410, 494, 555, 609
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 1 cut(s) 319
BtuMI TCGCGA 1 cut(s) 404
Cac8I GCNNGC 3 cut(s) 17, 644, 786
CaiI CAGNNNCTG 2 cut(s) 16, 43
Cfr13I GGNCC 2 cut(s) 260, 607
Csp6I GTAC 2 cut(s) 384, 717
CspCI CAANNNNNGTGG 2 cut(s) 400, 435
CviQI GTAC 2 cut(s) 384, 717
DdeI CTNAG 5 cut(s) 104, 224, 370, 625, 794
DpnI GATC 5 cut(s) 222, 358, 368, 401, 583
DpnII GATC 5 cut(s) 220, 356, 366, 399, 581
Eam1104I CTCTTC 4 cut(s) 446, 476, 481, 523
EarI CTCTTC 4 cut(s) 446, 476, 481, 523
Ecl136II GAGCTC 1 cut(s) 374
Eco147I AGGCCT 1 cut(s) 555
Eco24I GRGCYC 2 cut(s) 376, 656
Eco32I GATATC 1 cut(s) 183
Eco53kI GAGCTC 1 cut(s) 374
Eco57I CTGAAG 1 cut(s) 562
EcoICRI GAGCTC 1 cut(s) 374
EcoNI CCTNNNNNAGG 1 cut(s) 215
EcoRII CCWGG 4 cut(s) 210, 262, 555, 603
EcoRV GATATC 1 cut(s) 183
EcoT38I GRGCYC 2 cut(s) 376, 656
FaiI YATR 2 cut(s) 135, 722
FblI GTMKAC 1 cut(s) 173
Fnu4HI GCNGC 1 cut(s) 380
FriOI GRGCYC 2 cut(s) 376, 656
Fsp4HI GCNGC 1 cut(s) 380
GluI GCNGC 1 cut(s) 380
HaeIII GGCC 5 cut(s) 262, 410, 494, 555, 609
HapII CCGG 1 cut(s) 130
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HindIII AAGCTT 1 cut(s) 541
HinfI GANTC 6 cut(s) 157, 306, 325, 337, 437, 753
HpaII CCGG 1 cut(s) 130
HphI GGTGA 2 cut(s) 288, 361
Hpy166II GTNNAC 2 cut(s) 174, 279
Hpy188I TCNGA 6 cut(s) 225, 366, 371, 436, 475, 581
Hpy188III TCNNGA 9 cut(s) 246, 255, 291, 403, 501, 537, 594, 615, 750
Hpy8I GTNNAC 2 cut(s) 174, 279
Hpy99I CGWCG 1 cut(s) 175
HpyAV CCTTC 5 cut(s) 73, 297, 315, 400, 612
HpyCH4IV ACGT 1 cut(s) 469
HpyCH4V TGCA 4 cut(s) 312, 634, 660, 724
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 401, 730, 790
HpyF3I CTNAG 5 cut(s) 104, 224, 370, 625, 794
HpySE526I ACGT 1 cut(s) 469
Kzo9I GATC 5 cut(s) 220, 356, 366, 399, 581
LguI GCTCTTC 1 cut(s) 446
LmnI GCTCC 1 cut(s) 621
Lsp1109I GCAGC 1 cut(s) 366
MaeII ACGT 1 cut(s) 469
MalI GATC 5 cut(s) 222, 358, 368, 401, 583
MboI GATC 5 cut(s) 220, 356, 366, 399, 581
MfeI CAATTG 1 cut(s) 517
MflI RGATCY 2 cut(s) 220, 356
MhlI GDGCHC 3 cut(s) 245, 376, 656
MluCI AATT 9 cut(s) 97, 250, 332, 341, 496, 517, 547, 666, 676
MlyI GAGTC 4 cut(s) 300, 334, 446, 747
MroXI GAANNNNTTC 1 cut(s) 469
MseI TTAA 2 cut(s) 23, 550
MspI CCGG 1 cut(s) 130
MspR9I CCNGG 4 cut(s) 212, 264, 557, 605
MunI CAATTG 1 cut(s) 517
MvaI CCWGG 4 cut(s) 212, 264, 557, 605
MvnI CGCG 1 cut(s) 404
MwoI GCNNNNNNNGC 4 cut(s) 25, 401, 730, 790
NdeII GATC 5 cut(s) 220, 356, 366, 399, 581
NlaIV GGNNCC 1 cut(s) 92
NruI TCGCGA 1 cut(s) 404
PceI AGGCCT 1 cut(s) 555
PciSI GCTCTTC 1 cut(s) 446
PcsI WCGNNNNNNNCGW 1 cut(s) 287
PdmI GAANNNNTTC 1 cut(s) 469
PfeI GAWTC 2 cut(s) 157, 337
PkrI GCNGC 1 cut(s) 381
PleI GAGTC 4 cut(s) 300, 333, 445, 747
PpsI GAGTC 4 cut(s) 300, 333, 445, 747
Psp124BI GAGCTC 1 cut(s) 376
Psp6I CCWGG 4 cut(s) 210, 262, 555, 603
PspGI CCWGG 4 cut(s) 210, 262, 555, 603
PspN4I GGNNCC 1 cut(s) 92
PspPI GGNCC 2 cut(s) 260, 607
PstI CTGCAG 1 cut(s) 314
PstNI CAGNNNCTG 2 cut(s) 16, 43
PsuI RGATCY 2 cut(s) 220, 356
RruI TCGCGA 1 cut(s) 404
RsaI GTAC 2 cut(s) 385, 718
RsaNI GTAC 2 cut(s) 384, 717
SacI GAGCTC 1 cut(s) 376
SalI GTCGAC 1 cut(s) 172
SapI GCTCTTC 1 cut(s) 446
SaqAI TTAA 2 cut(s) 23, 550
SatI GCNGC 1 cut(s) 380
Sau3AI GATC 5 cut(s) 220, 356, 366, 399, 581
Sau96I GGNCC 2 cut(s) 260, 607
SchI GAGTC 4 cut(s) 300, 334, 446, 747
ScrFI CCNGG 4 cut(s) 212, 264, 557, 605
SduI GDGCHC 3 cut(s) 245, 376, 656
SetI ASST 9 cut(s) 278, 376, 458, 472, 508, 545, 593, 626, 648
SfcI CTRYAG 1 cut(s) 310
SmlI CTYRAG 3 cut(s) 255, 501, 592
SmoI CTYRAG 3 cut(s) 255, 501, 592
Sse9I AATT 9 cut(s) 97, 250, 332, 341, 496, 517, 547, 666, 676
SseBI AGGCCT 1 cut(s) 555
SsiI CCGC 1 cut(s) 791
SspI AATATT 1 cut(s) 693
SstI GAGCTC 1 cut(s) 376
StuI AGGCCT 1 cut(s) 555
StyD4I CCNGG 4 cut(s) 210, 262, 555, 603
TaiI ACGT 1 cut(s) 472
TaqI TCGA 3 cut(s) 173, 290, 328
TasI AATT 9 cut(s) 97, 250, 332, 341, 496, 517, 547, 666, 676
TfiI GAWTC 2 cut(s) 157, 337
Tru1I TTAA 2 cut(s) 23, 550
Tru9I TTAA 2 cut(s) 23, 550
TscAI CASTG 1 cut(s) 319
TseI GCWGC 1 cut(s) 379
TspDTI ATGAA 2 cut(s) 494, 687
TspRI CASTG 1 cut(s) 319
XagI CCTNNNNNAGG 1 cut(s) 215
XapI RAATTY 1 cut(s) 250
XmiI GTMKAC 1 cut(s) 173
XmnI GAANNNNTTC 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.