Prupe.8G223200_v2.0.a1

Patellin-4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0001644
Physical Location & Seq
Forward (+)
20178831 .. 20181212
2382 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G223200.1

Sequence Viewer

Length: 1431 bp
ATGACAGAGAAAAAGAATGAGCAGAACCCAAAAGAGGAGGGTGATCATGTCAGAGAAAAAGAGGAGAGCCAAAGCAGCCAAGAAGATGATCAAGAAGAAAGCAAGAAGAGCCCAAGAGAGCAAAACCCTAATAATAGTGGAAACGAAAGCACCCAGGAAGATGAAGAAAGTGACATAGAATTTCTTGGCCCTGCAATTTATCAAGAGGCTGAAACCACACCTTGTGTTGAATTGAAGCAGAGCGACAACAAGAAAAAGAATAAAGCTTTATTGGAATTGAGGTGCAGGGTGGAAGATGCAATTGTTGGGAATTATCTTCTGGGAAAACCCAACAAAATGATTAGCCCCGCTGAAAGTGCCAGGCAGAGAGAAGAGCTTAGGGAGATTAGTCTTTGGGGTGTACCTTTGTTGCCTAGCAAAGGTCACCAAGGCACTGACATTGTGCTGCTCAAATTCTTGAGAGCCAAAGATTTTAAGGTCCTTGATGCATTCGAGATGCTCCGGAGGACGCTGAAATGGCGAAAAGAATACAGAACCGATGAGATTCTTGAAGAAGAGTTGGGGTCTGATCTTGAAAATTTGGTGTTTTGGAGTAGTGTGGACAAGGAGGGGCACCCTTTGTGTTTCACTGTGTATGGGCCTTTCAAGGAGAGGGAATTGTACAAGAGGACATTTGGTTCTAAAGAGAAACGCCAGCAGTTTTTAAGGTGGAGGGTTCAATTTATGGAAAAGGGCATCAAGAAGCTCAGCTTCAAGAAAGGAGGAGTGGACTCTATGGTTCACATTACAGATTTAAAGAACTCGCCGGGGCCGGATATGAAGGAGCTTCGTTCTCTTAGCAGCAAAACTCTGGTGTTGCTTCAGGAAAATTATCCAGAGCTCATTCAAAAGAATATTGTCATAAATGCTCCACTTTGGTACTATGTGGCACATGTTCTCAGATCAAGGCTCCTCAGTCAGAGAACTAAAAAGAAGTTCGTGTTTGCCAGGCCTTCAAATGTTACAAAGACCCTTCTCAAGTTCGTAGACCCGGAAAAACTCCCAGTTCAATATGGTGGCCTCAAAAGAGAGCAAGATCCTGAATTCACACCAGAAGACAAAGCATCAAGGCGCTCAGTCAAAGCAAACACAACTGCCTGCATTGAAATCCCAGTTGCTGAGGGTGGTTTGACAATGGTGTGGGAGCTAACAGTGGTGGGATGGGATGTATCCTACAAGGAGGAGTTTGTTCCTGATGATGAATGCTCGTACAAGATTCTGCTCCAAAACAAGAAGAGATTAGGTCAATGTGTGAGAAATTCCTTCTATGCTAATGAACCAGGGAAGATTGTGATCACCATTGAGAATTGGACCTTCAAGAACAAGAGGGTTCTCTACAGATCCAAATCCAAACTCACTGTTCCTGTGTACAGATTCTTGTTTAACAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

477

Amino Acids

55.36

Weight (kDa)

8.86

Isoelectric Point (pI)

59.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 612
AccI GTMKAC 1 cut(s) 1026
AccIII TCCGGA 1 cut(s) 501
AciI CCGC 1 cut(s) 348
AclWI GGATC 2 cut(s) 1070, 1374
AcsI RAATTY 5 cut(s) 179, 452, 577, 1082, 1297
AcuI CTGAAG 1 cut(s) 845
AdeI CACNNNGTG 1 cut(s) 224
AfaI GTAC 5 cut(s) 402, 662, 920, 1250, 1409
AfiI CCNNNNNNNGG 2 cut(s) 34, 419
AflIII ACRYGT 1 cut(s) 931
AjnI CCWGG 4 cut(s) 153, 359, 986, 1318
AloI GAACNNNNNNTCC 2 cut(s) 661, 693
AluBI AGCT 7 cut(s) 266, 376, 745, 750, 826, 880, 1186
AluI AGCT 7 cut(s) 266, 376, 745, 750, 826, 880, 1186
Alw21I GWGCWC 1 cut(s) 882
AlwI GGATC 2 cut(s) 1070, 1374
AlwNI CAGNNNCTG 1 cut(s) 1157
Aor13HI TCCGGA 1 cut(s) 501
AoxI GGCC 5 cut(s) 187, 638, 809, 989, 1057
ApeKI GCWGC 3 cut(s) 75, 445, 840
ApoI RAATTY 5 cut(s) 179, 452, 577, 1082, 1297
AspLEI GCGC 1 cut(s) 1113
AspS9I GGNCC 5 cut(s) 188, 478, 638, 809, 1350
AsuC2I CCSGG 2 cut(s) 807, 1031
AsuHPI GGTGA 3 cut(s) 53, 416, 1327
AvaII GGWCC 2 cut(s) 478, 1350
BaeGI GKGCMC 1 cut(s) 615
BanI GGYRCC 1 cut(s) 612
BanII GRGCYC 2 cut(s) 113, 882
BbsI GAAGAC 1 cut(s) 1101
Bbv12I GWGCWC 1 cut(s) 882
BbvCI CCTCAGC 1 cut(s) 1158
BbvI GCAGC 3 cut(s) 87, 432, 852
BccI CCATC 1 cut(s) 1194
BciT130I CCWGG 4 cut(s) 155, 361, 988, 1320
BciVI GTATCC 1 cut(s) 1219
BclI TGATCA 3 cut(s) 43, 88, 1332
BcnI CCSGG 2 cut(s) 807, 1031
BfaI CTAG 1 cut(s) 414
BfmI CTRYAG 1 cut(s) 1375
BfoI RGCGCY 1 cut(s) 1114
BfuI GTATCC 1 cut(s) 1219
BisI GCNGC 3 cut(s) 76, 446, 841
BlpI GCTNAGC 1 cut(s) 746
BlsI GCNGC 3 cut(s) 77, 447, 842
Bme1390I CCNGG 6 cut(s) 155, 361, 807, 988, 1031, 1320
Bme18I GGWCC 2 cut(s) 478, 1350
BmgT120I GGNCC 5 cut(s) 188, 478, 638, 809, 1350
BmiI GGNNCC 3 cut(s) 614, 810, 950
BmrFI CCNGG 6 cut(s) 155, 361, 807, 988, 1031, 1320
BmrI ACTGGG 2 cut(s) 1037, 1145
BmsI GCATC 5 cut(s) 286, 475, 486, 744, 1112
BmuI ACTGGG 2 cut(s) 1037, 1145
BpiI GAAGAC 1 cut(s) 1101
Bpu10I CCTNAGC 2 cut(s) 377, 1158
Bpu1102I GCTNAGC 1 cut(s) 746
BpuEI CTTGAG 2 cut(s) 478, 1001
BpuMI CCSGG 2 cut(s) 807, 1031
BsaBI GATNNNNATC 2 cut(s) 1331, 1384
BsaJI CCNNGG 4 cut(s) 153, 427, 806, 1319
BsaWI WCCGGW 1 cut(s) 501
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 419
Bse1I ACTGG 2 cut(s) 1043, 1151
Bse8I GATNNNNATC 2 cut(s) 1331, 1384
BseAI TCCGGA 1 cut(s) 501
BseBI CCWGG 4 cut(s) 155, 361, 988, 1320
BseDI CCNNGG 4 cut(s) 153, 427, 806, 1319
BseGI GGATG 2 cut(s) 1205, 1210
BseJI GATNNNNATC 2 cut(s) 1331, 1384
BseLI CCNNNNNNNGG 2 cut(s) 34, 419
BseMII CTCAG 5 cut(s) 760, 952, 967, 1128, 1149
BseNI ACTGG 2 cut(s) 1043, 1151
BseRI GAGGAG 5 cut(s) 50, 77, 777, 941, 1235
BseSI GKGCMC 1 cut(s) 615
BseXI GCAGC 3 cut(s) 87, 432, 852
BsgI GTGCAG 1 cut(s) 304
BshFI GGCC 5 cut(s) 189, 640, 811, 991, 1059
BshNI GGYRCC 1 cut(s) 612
BsiHKAI GWGCWC 1 cut(s) 882
BsiSI CCGG 4 cut(s) 502, 806, 812, 1031
BslI CCNNNNNNNGG 2 cut(s) 34, 419
BsmI GAATGC 2 cut(s) 488, 1247
BsnI GGCC 5 cut(s) 189, 640, 811, 991, 1059
Bsp1286I GDGCHC 3 cut(s) 113, 615, 882
Bsp13I TCCGGA 1 cut(s) 501
Bsp1407I TGTACA 2 cut(s) 660, 1407
Bsp143I GATC 7 cut(s) 43, 88, 568, 941, 1075, 1332, 1379
Bsp1720I GCTNAGC 1 cut(s) 746
BspACI CCGC 1 cut(s) 348
BspANI GGCC 5 cut(s) 189, 640, 811, 991, 1059
BspCNI CTCAG 5 cut(s) 759, 951, 966, 1127, 1150
BspEI TCCGGA 1 cut(s) 501
BspLI GGNNCC 3 cut(s) 614, 810, 950
BspPI GGATC 2 cut(s) 1070, 1374
BspQI GCTCTTC 2 cut(s) 101, 366
BspT107I GGYRCC 1 cut(s) 612
BsrGI TGTACA 2 cut(s) 660, 1407
BsrI ACTGG 2 cut(s) 1043, 1151
BssECI CCNNGG 4 cut(s) 153, 427, 806, 1319
BssMI GATC 7 cut(s) 43, 88, 568, 941, 1075, 1332, 1379
BssT1I CCWWGG 1 cut(s) 427
Bst2UI CCWGG 4 cut(s) 155, 361, 988, 1320
Bst4CI ACNGT 3 cut(s) 631, 1192, 1399
Bst6I CTCTTC 4 cut(s) 101, 366, 549, 1268
BstAUI TGTACA 2 cut(s) 660, 1407
BstC8I GCNNGC 2 cut(s) 695, 1138
BstDEI CTNAG 7 cut(s) 377, 746, 836, 938, 953, 1114, 1158
BstEII GGTNACC 1 cut(s) 422
BstENI CCTNNNNNAGG 1 cut(s) 417
BstF5I GGATG 2 cut(s) 1205, 1210
BstH2I RGCGCY 1 cut(s) 1114
BstHHI GCGC 1 cut(s) 1113
BstKTI GATC 7 cut(s) 46, 91, 571, 944, 1078, 1335, 1382
BstMBI GATC 7 cut(s) 43, 88, 568, 941, 1075, 1332, 1379
BstMWI GCNNNNNNNGC 4 cut(s) 75, 108, 356, 517
BstNI CCWGG 4 cut(s) 155, 361, 988, 1320
BstNSI RCATGY 1 cut(s) 935
BstPI GGTNACC 1 cut(s) 422
BstSCI CCNGG 6 cut(s) 153, 359, 805, 986, 1029, 1318
BstSFI CTRYAG 1 cut(s) 1375
BstSLI GKGCMC 1 cut(s) 615
BstV1I GCAGC 3 cut(s) 87, 432, 852
BstV2I GAAGAC 1 cut(s) 1101
BstX2I RGATCY 2 cut(s) 1075, 1379
BstYI RGATCY 2 cut(s) 1075, 1379
BsuI GTATCC 1 cut(s) 1219
BsuRI GGCC 5 cut(s) 189, 640, 811, 991, 1059
BtsCI GGATG 2 cut(s) 1205, 1210
BtsIMutI CAGTG 4 cut(s) 432, 627, 1197, 1395
Cac8I GCNNGC 2 cut(s) 695, 1138
CaiI CAGNNNCTG 1 cut(s) 1157
CfoI GCGC 1 cut(s) 1113
Cfr13I GGNCC 5 cut(s) 188, 478, 638, 809, 1350
CseI GACGC 1 cut(s) 517
Csp6I GTAC 5 cut(s) 401, 661, 919, 1249, 1408
CviAII CATG 2 cut(s) 47, 932
CviQI GTAC 5 cut(s) 401, 661, 919, 1249, 1408
DdeI CTNAG 7 cut(s) 377, 746, 836, 938, 953, 1114, 1158
DpnI GATC 7 cut(s) 45, 90, 570, 943, 1077, 1334, 1381
DpnII GATC 7 cut(s) 43, 88, 568, 941, 1075, 1332, 1379
DraI TTTAAA 1 cut(s) 795
DraIII CACNNNGTG 1 cut(s) 224
Eam1104I CTCTTC 4 cut(s) 101, 366, 549, 1268
EarI CTCTTC 4 cut(s) 101, 366, 549, 1268
Ecl136II GAGCTC 1 cut(s) 880
Eco130I CCWWGG 1 cut(s) 427
Eco147I AGGCCT 1 cut(s) 991
Eco24I GRGCYC 2 cut(s) 113, 882
Eco47I GGWCC 2 cut(s) 478, 1350
Eco53kI GAGCTC 1 cut(s) 880
Eco57I CTGAAG 1 cut(s) 845
Eco91I GGTNACC 1 cut(s) 422
EcoICRI GAGCTC 1 cut(s) 880
EcoNI CCTNNNNNAGG 1 cut(s) 417
EcoO109I RGGNCCY 1 cut(s) 478
EcoO65I GGTNACC 1 cut(s) 422
EcoRI GAATTC 1 cut(s) 1082
EcoRII CCWGG 4 cut(s) 153, 359, 986, 1318
EcoT14I CCWWGG 1 cut(s) 427
EcoT22I ATGCAT 1 cut(s) 490
EcoT38I GRGCYC 2 cut(s) 113, 882
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 2 cut(s) 50, 935
FalI AAGNNNNNCTT 2 cut(s) 734, 766
FatI CATG 2 cut(s) 46, 931
FauI CCCGC 1 cut(s) 355
FbaI TGATCA 3 cut(s) 43, 88, 1332
FblI GTMKAC 1 cut(s) 1026
Fnu4HI GCNGC 3 cut(s) 76, 446, 841
FokI GGATG 2 cut(s) 1212, 1217
FriOI GRGCYC 2 cut(s) 113, 882
Fsp4HI GCNGC 3 cut(s) 76, 446, 841
FspBI CTAG 1 cut(s) 414
GlaI GCGC 1 cut(s) 1112
GluI GCNGC 3 cut(s) 76, 446, 841
HaeII RGCGCY 1 cut(s) 1114
HaeIII GGCC 5 cut(s) 189, 640, 811, 991, 1059
HapII CCGG 4 cut(s) 502, 806, 812, 1031
HgaI GACGC 1 cut(s) 517
HhaI GCGC 1 cut(s) 1113
Hin1II CATG 2 cut(s) 50, 935
Hin6I GCGC 1 cut(s) 1111
HinP1I GCGC 1 cut(s) 1111
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 4 cut(s) 544, 770, 1255, 1413
HpaII CCGG 4 cut(s) 502, 806, 812, 1031
HphI GGTGA 3 cut(s) 53, 416, 1327
Hpy166II GTNNAC 6 cut(s) 401, 601, 769, 781, 1027, 1408
Hpy188I TCNGA 4 cut(s) 53, 568, 941, 960
Hpy8I GTNNAC 6 cut(s) 401, 601, 769, 781, 1027, 1408
HpyAV CCTTC 5 cut(s) 814, 1002, 1022, 1312, 1363
HpyCH4III ACNGT 3 cut(s) 631, 1192, 1399
HpyCH4V TGCA 5 cut(s) 194, 285, 299, 488, 1140
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 108, 356, 517
HpyF3I CTNAG 7 cut(s) 377, 746, 836, 938, 953, 1114, 1158
Hsp92II CATG 2 cut(s) 50, 935
HspAI GCGC 1 cut(s) 1111
Kpn2I TCCGGA 1 cut(s) 501
Ksp22I TGATCA 3 cut(s) 43, 88, 1332
Kzo9I GATC 7 cut(s) 43, 88, 568, 941, 1075, 1332, 1379
LguI GCTCTTC 2 cut(s) 101, 366
LmnI GCTCC 6 cut(s) 504, 823, 913, 954, 1183, 1266
Lsp1109I GCAGC 3 cut(s) 87, 432, 852
LweI GCATC 5 cut(s) 286, 475, 486, 744, 1112
MaeI CTAG 1 cut(s) 414
MaeIII GTNAC 3 cut(s) 170, 422, 1000
MalI GATC 7 cut(s) 45, 90, 570, 943, 1077, 1334, 1381
MboI GATC 7 cut(s) 43, 88, 568, 941, 1075, 1332, 1379
MfeI CAATTG 1 cut(s) 300
MflI RGATCY 2 cut(s) 1075, 1379
MhlI GDGCHC 3 cut(s) 113, 615, 882
MlyI GAGTC 1 cut(s) 764
Mph1103I ATGCAT 1 cut(s) 490
MroI TCCGGA 1 cut(s) 501
MseI TTAA 4 cut(s) 474, 704, 794, 1422
MspA1I CMGCKG 1 cut(s) 350
MspI CCGG 4 cut(s) 502, 806, 812, 1031
MspR9I CCNGG 6 cut(s) 155, 361, 807, 988, 1031, 1320
MunI CAATTG 1 cut(s) 300
Mva1269I GAATGC 2 cut(s) 488, 1247
MvaI CCWGG 4 cut(s) 155, 361, 988, 1320
MwoI GCNNNNNNNGC 4 cut(s) 75, 108, 356, 517
NciI CCSGG 2 cut(s) 807, 1031
NdeII GATC 7 cut(s) 43, 88, 568, 941, 1075, 1332, 1379
NlaIII CATG 2 cut(s) 50, 935
NlaIV GGNNCC 3 cut(s) 614, 810, 950
NmuCI GTSAC 2 cut(s) 170, 422
NsiI ATGCAT 1 cut(s) 490
NspI RCATGY 1 cut(s) 935
PceI AGGCCT 1 cut(s) 991
PciI ACATGT 1 cut(s) 931
PciSI GCTCTTC 2 cut(s) 101, 366
PctI GAATGC 2 cut(s) 488, 1247
PfeI GAWTC 3 cut(s) 544, 1255, 1413
PkrI GCNGC 3 cut(s) 77, 447, 842
PleI GAGTC 1 cut(s) 764
PpsI GAGTC 1 cut(s) 764
PpuMI RGGWCCY 1 cut(s) 478
PscI ACATGT 1 cut(s) 931
Psp124BI GAGCTC 1 cut(s) 882
Psp5II RGGWCCY 1 cut(s) 478
Psp6I CCWGG 4 cut(s) 153, 359, 986, 1318
PspEI GGTNACC 1 cut(s) 422
PspGI CCWGG 4 cut(s) 153, 359, 986, 1318
PspN4I GGNNCC 3 cut(s) 614, 810, 950
PspPI GGNCC 5 cut(s) 188, 478, 638, 809, 1350
PspPPI RGGWCCY 1 cut(s) 478
PstNI CAGNNNCTG 1 cut(s) 1157
PsuI RGATCY 2 cut(s) 1075, 1379
RsaI GTAC 5 cut(s) 402, 662, 920, 1250, 1409
RsaNI GTAC 5 cut(s) 401, 661, 919, 1249, 1408
SacI GAGCTC 1 cut(s) 882
SapI GCTCTTC 2 cut(s) 101, 366
SaqAI TTAA 4 cut(s) 474, 704, 794, 1422
SatI GCNGC 3 cut(s) 76, 446, 841
Sau3AI GATC 7 cut(s) 43, 88, 568, 941, 1075, 1332, 1379
Sau96I GGNCC 5 cut(s) 188, 478, 638, 809, 1350
SchI GAGTC 1 cut(s) 764
ScrFI CCNGG 6 cut(s) 155, 361, 807, 988, 1031, 1320
SduI GDGCHC 3 cut(s) 113, 615, 882
SfaNI GCATC 5 cut(s) 286, 475, 486, 744, 1112
SfcI CTRYAG 1 cut(s) 1375
SinI GGWCC 2 cut(s) 478, 1350
SmlI CTYRAG 2 cut(s) 457, 1016
SmoI CTYRAG 2 cut(s) 457, 1016
SseBI AGGCCT 1 cut(s) 991
SsiI CCGC 1 cut(s) 348
SspI AATATT 1 cut(s) 895
SspMI CTAG 1 cut(s) 414
SstI GAGCTC 1 cut(s) 882
StuI AGGCCT 1 cut(s) 991
StyD4I CCNGG 6 cut(s) 153, 359, 805, 986, 1029, 1318
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 3 cut(s) 631, 1192, 1399
TaqI TCGA 1 cut(s) 492
TatI WGTACW 2 cut(s) 660, 1407
TfiI GAWTC 3 cut(s) 544, 1255, 1413
Tru1I TTAA 4 cut(s) 474, 704, 794, 1422
Tru9I TTAA 4 cut(s) 474, 704, 794, 1422
TscAI CASTG 4 cut(s) 439, 634, 1197, 1402
TseFI GTSAC 2 cut(s) 170, 422
TseI GCWGC 3 cut(s) 75, 445, 840
Tsp45I GTSAC 2 cut(s) 170, 422
TspDTI ATGAA 4 cut(s) 177, 833, 1254, 1329
TspRI CASTG 4 cut(s) 439, 634, 1197, 1402
VpaK11BI GGWCC 2 cut(s) 478, 1350
XagI CCTNNNNNAGG 1 cut(s) 417
XapI RAATTY 5 cut(s) 179, 452, 577, 1082, 1297
XceI RCATGY 1 cut(s) 935
XmiI GTMKAC 1 cut(s) 1026
XspI CTAG 1 cut(s) 414
Zsp2I ATGCAT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.