Rmu_sc0013020.1_g000005

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0013020.1
OG0001644
Physical Location & Seq
Forward (+)
26727 .. 30321
3595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0013020.1_g000005.1.cds

Sequence Viewer

Length: 1623 bp
atgactgtggaggagacccaggtggcggcggcggcggcggctgaggtcgtggaggttgaggagccggtgaaggaggtggaggagtgcgagaaggctgttgttgaggaatccgagtccaagccaaccactgttgagaagagcacttcttataaggaggagagcaactatctctctgatctcaaggagtttgagaagaaggctttggttgagctcaaatcgaagctcgaagaagccattttgggcaacaacattttcaagaaagatgagcccaagaaggttgaggagaaggtgaaggcggtagctgaggtcgaggagaagaaggaagagaaaacagaggctgctgcgaaaacaacagaggaaacagaggctgctgtgaaagcagaggaaacagagcctgctgtgaaatcagaggaaacagagcctgctgttaaagcagaggaaacagagcctgctgttaaagcagaggaaacagaggctgttgtgaaaacagagtgtgctgaaaaaacagaggaaggagaaaaggcaccagaattggctgagaagaaagaagaagtagcagttccggtatgcgaggaagaagcgaaacccgaatccagcgaagtcgtcgacacagatatctcactctggggtgtgcctctgttccctggaaaaggatctgagggaactgatgtggtgctcttgaaattcttgagggccagggagttcaaggtgaacgaagccttcgagatgttgaagaagactctgcagtggaggaaggagtcgaaaattgattcaattttgggtgaggagatctgctctgatctgagctctgctgcgtacttgaacggcgtggatcgcgaaggccaccctgtttgctacaacatcttcggagtttttgacaacgaagagctttaccagaagacgttcggaaatgaagagaagagaggccaattcttgaggtggagggttcaattgatggagaagagtatccagaaacttgaattaaggcctggtggtgctacttctctgcttcagatcaatgacctcaagaactcgcctgggccaatcaagaaggagcttagggttgcaacaaagcaagctgttgggctcttgcaggacaattaccctgaattggttgccaaaaatattttcatcaatgttccattctggtactatgcactcaatgctctgttgtctccattcttgactcaaagaaccaagagcaagtttgtggtggctcgccccgctaaggtcaccgagacccttctcaagtacattccagctgaggagataccggttcagtatggtggcttcaagagagaaaatgacaccgagttcttctcctccgaagatggttctgtttctgagctaactctcaaggccggatcaacccaaaccatagagattgatctagctgaggttgataatagcacattggtttgggatgtgagtgttttgggatgggaagtgaattacaaggaggagtttgttcctagtgatgaagggtcatacaccattattgttcagaagaagaagaagatcggagctcatgaagggcctattcgcaacactttccgaagcaatgagcctggaaagggttttagacatggtatggccccaaatgtcaaatgggaagcatactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

540

Amino Acids

60.5

Weight (kDa)

4.79

Isoelectric Point (pI)

52.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 150
AccB1I GGYRCC 1 cut(s) 523
AccI GTMKAC 1 cut(s) 606
AccII CGCG 1 cut(s) 837
AciI CCGC 7 cut(s) 26, 29, 32, 35, 38, 296, 1224
AclWI GGATC 3 cut(s) 661, 840, 1372
AcsI RAATTY 1 cut(s) 683
AcuI CTGAAG 1 cut(s) 995
AfaI GTAC 3 cut(s) 818, 1151, 1253
AfiI CCNNNNNNNGG 5 cut(s) 25, 650, 1111, 1228, 1574
AgeI ACCGGT 1 cut(s) 1273
AgsI TTSAA 9 cut(s) 256, 682, 706, 733, 774, 823, 950, 980, 1294
AjnI CCWGG 6 cut(s) 18, 643, 695, 988, 1036, 1567
AjuI GAANNNNNNNTTGG 4 cut(s) 185, 217, 1112, 1144
Alw21I GWGCWC 5 cut(s) 143, 213, 678, 809, 1528
Alw26I GTCTC 3 cut(s) 8, 1179, 1232
AlwI GGATC 3 cut(s) 661, 840, 1372
AlwNI CAGNNNCTG 6 cut(s) 338, 368, 395, 422, 449, 476
AoxI GGCC 8 cut(s) 693, 841, 925, 986, 1040, 1359, 1535, 1593
ApeKI GCWGC 4 cut(s) 338, 341, 368, 812
ApoI RAATTY 1 cut(s) 683
AsiGI ACCGGT 1 cut(s) 1273
Asp700I GAANNNNTTC 1 cut(s) 902
AspS9I GGNCC 4 cut(s) 693, 1040, 1535, 1594
AsuHPI GGTGA 5 cut(s) 79, 301, 721, 794, 1225
BanI GGYRCC 1 cut(s) 523
BanII GRGCYC 5 cut(s) 213, 270, 809, 1089, 1528
BarI GAAGNNNNNNTAC 2 cut(s) 1274, 1306
BbsI GAAGAC 2 cut(s) 743, 905
Bbv12I GWGCWC 5 cut(s) 143, 213, 678, 809, 1528
BbvCI CCTCAGC 4 cut(s) 42, 303, 1263, 1395
BbvI GCAGC 4 cut(s) 325, 328, 355, 799
BccI CCATC 3 cut(s) 949, 1325, 1434
BceAI ACGGC 1 cut(s) 841
BciT130I CCWGG 6 cut(s) 20, 645, 697, 990, 1038, 1569
BciVI GTATCC 1 cut(s) 977
BcoDI GTCTC 3 cut(s) 8, 1179, 1232
BfaI CTAG 2 cut(s) 1391, 1473
BfmI CTRYAG 1 cut(s) 743
BfuI GTATCC 1 cut(s) 977
BglII AGATCT 1 cut(s) 789
BisI GCNGC 9 cut(s) 27, 30, 33, 36, 39, 339, 342, 369, 813
BlsI GCNGC 9 cut(s) 28, 31, 34, 37, 40, 340, 343, 370, 814
Bme1390I CCNGG 6 cut(s) 20, 645, 697, 990, 1038, 1569
BmgT120I GGNCC 4 cut(s) 693, 1040, 1535, 1594
BmiI GGNNCC 3 cut(s) 63, 525, 1596
BmrFI CCNGG 6 cut(s) 20, 645, 697, 990, 1038, 1569
BpiI GAAGAC 2 cut(s) 743, 905
BplI GAGNNNNNCTC 4 cut(s) 779, 811, 1304, 1336
Bpu10I CCTNAGC 6 cut(s) 42, 303, 1058, 1227, 1263, 1395
BpuEI CTTGAG 6 cut(s) 164, 709, 955, 1010, 1232, 1340
BsaI GGTCTC 2 cut(s) 8, 1232
BsaJI CCNNGG 4 cut(s) 18, 643, 696, 1037
BsaWI WCCGGW 2 cut(s) 562, 1273
Bsc4I CCNNNNNNNGG 5 cut(s) 25, 650, 1111, 1228, 1574
Bse118I RCCGGY 2 cut(s) 64, 1273
Bse3DI GCAATG 1 cut(s) 1567
BseBI CCWGG 6 cut(s) 20, 645, 697, 990, 1038, 1569
BseDI CCNNGG 4 cut(s) 18, 643, 696, 1037
BseGI GGATG 2 cut(s) 1429, 1445
BseLI CCNNNNNNNGG 5 cut(s) 25, 650, 1111, 1228, 1574
BseMI GCAATG 1 cut(s) 1567
BseMII CTCAG 8 cut(s) 33, 294, 528, 648, 794, 1254, 1335, 1386
BseXI GCAGC 4 cut(s) 325, 328, 355, 799
Bsh1236I CGCG 1 cut(s) 837
BshFI GGCC 8 cut(s) 695, 843, 927, 988, 1042, 1361, 1537, 1595
BshNI GGYRCC 1 cut(s) 523
BshTI ACCGGT 1 cut(s) 1273
BsiHKAI GWGCWC 5 cut(s) 143, 213, 678, 809, 1528
BsiSI CCGG 4 cut(s) 65, 563, 1274, 1362
BslI CCNNNNNNNGG 5 cut(s) 25, 650, 1111, 1228, 1574
BsmAI GTCTC 3 cut(s) 8, 1179, 1232
BsnI GGCC 8 cut(s) 695, 843, 927, 988, 1042, 1361, 1537, 1595
Bso31I GGTCTC 2 cut(s) 8, 1232
Bsp1286I GDGCHC 7 cut(s) 143, 213, 270, 678, 809, 1089, 1528
Bsp143I GATC 9 cut(s) 175, 653, 789, 799, 832, 1014, 1364, 1387, 1518
Bsp68I TCGCGA 1 cut(s) 837
BspACI CCGC 7 cut(s) 26, 29, 32, 35, 38, 296, 1224
BspANI GGCC 8 cut(s) 695, 843, 927, 988, 1042, 1361, 1537, 1595
BspCNI CTCAG 8 cut(s) 34, 295, 529, 649, 795, 1255, 1336, 1387
BspFNI CGCG 1 cut(s) 837
BspHI TCATGA 1 cut(s) 1528
BspLI GGNNCC 3 cut(s) 63, 525, 1596
BspMAI CTGCAG 1 cut(s) 747
BspPI GGATC 3 cut(s) 661, 840, 1372
BspQI GCTCTTC 2 cut(s) 131, 879
BspT107I GGYRCC 1 cut(s) 523
BspTNI GGTCTC 2 cut(s) 8, 1232
BsrDI GCAATG 1 cut(s) 1567
BsrFI RCCGGY 2 cut(s) 64, 1273
BssAI RCCGGY 2 cut(s) 64, 1273
BssECI CCNNGG 4 cut(s) 18, 643, 696, 1037
BssMI GATC 9 cut(s) 175, 653, 789, 799, 832, 1014, 1364, 1387, 1518
Bst2UI CCWGG 6 cut(s) 20, 645, 697, 990, 1038, 1569
Bst4CI ACNGT 2 cut(s) 7, 130
Bst6I CTCTTC 6 cut(s) 131, 318, 879, 909, 914, 956
BstAPI GCANNNNNTGC 1 cut(s) 1163
BstC8I GCNNGC 5 cut(s) 396, 423, 450, 1077, 1219
BstEII GGTNACC 1 cut(s) 1231
BstENI CCTNNNNNAGG 1 cut(s) 648
BstF5I GGATG 2 cut(s) 1429, 1445
BstFNI CGCG 1 cut(s) 837
BstKTI GATC 9 cut(s) 178, 656, 792, 802, 835, 1017, 1367, 1390, 1521
BstMAI GTCTC 3 cut(s) 8, 1179, 1232
BstMBI GATC 9 cut(s) 175, 653, 789, 799, 832, 1014, 1364, 1387, 1518
BstMWI GCNNNNNNNGC 9 cut(s) 32, 35, 38, 377, 431, 458, 834, 1163, 1223
BstNI CCWGG 6 cut(s) 20, 645, 697, 990, 1038, 1569
BstPI GGTNACC 1 cut(s) 1231
BstSCI CCNGG 6 cut(s) 18, 643, 695, 988, 1036, 1567
BstSFI CTRYAG 1 cut(s) 743
BstUI CGCG 1 cut(s) 837
BstV1I GCAGC 4 cut(s) 325, 328, 355, 799
BstV2I GAAGAC 2 cut(s) 743, 905
BstX2I RGATCY 2 cut(s) 653, 789
BstYI RGATCY 2 cut(s) 653, 789
BsuI GTATCC 1 cut(s) 977
BsuRI GGCC 8 cut(s) 695, 843, 927, 988, 1042, 1361, 1537, 1595
BtsCI GGATG 2 cut(s) 1429, 1445
BtsI GCAGTG 1 cut(s) 752
BtsIMutI CAGTG 2 cut(s) 126, 752
BtuMI TCGCGA 1 cut(s) 837
Cac8I GCNNGC 5 cut(s) 396, 423, 450, 1077, 1219
CaiI CAGNNNCTG 6 cut(s) 338, 368, 395, 422, 449, 476
CciI TCATGA 1 cut(s) 1528
Cfr10I RCCGGY 2 cut(s) 64, 1273
Cfr13I GGNCC 4 cut(s) 693, 1040, 1535, 1594
Csp6I GTAC 3 cut(s) 817, 1150, 1252
CspAI ACCGGT 1 cut(s) 1273
CspCI CAANNNNNGTGG 2 cut(s) 833, 868
CviAII CATG 2 cut(s) 1529, 1586
CviQI GTAC 3 cut(s) 817, 1150, 1252
DpnI GATC 9 cut(s) 177, 655, 791, 801, 834, 1016, 1366, 1389, 1520
DpnII GATC 9 cut(s) 175, 653, 789, 799, 832, 1014, 1364, 1387, 1518
Eam1104I CTCTTC 6 cut(s) 131, 318, 879, 909, 914, 956
EarI CTCTTC 6 cut(s) 131, 318, 879, 909, 914, 956
Ecl136II GAGCTC 3 cut(s) 211, 807, 1526
Eco147I AGGCCT 1 cut(s) 988
Eco24I GRGCYC 5 cut(s) 213, 270, 809, 1089, 1528
Eco31I GGTCTC 2 cut(s) 8, 1232
Eco32I GATATC 1 cut(s) 616
Eco53kI GAGCTC 3 cut(s) 211, 807, 1526
Eco57I CTGAAG 1 cut(s) 995
Eco91I GGTNACC 1 cut(s) 1231
EcoICRI GAGCTC 3 cut(s) 211, 807, 1526
EcoNI CCTNNNNNAGG 1 cut(s) 648
EcoO109I RGGNCCY 1 cut(s) 1535
EcoO65I GGTNACC 1 cut(s) 1231
EcoRII CCWGG 6 cut(s) 18, 643, 695, 988, 1036, 1567
EcoRV GATATC 1 cut(s) 616
EcoT38I GRGCYC 5 cut(s) 213, 270, 809, 1089, 1528
FaeI CATG 2 cut(s) 1532, 1589
FatI CATG 2 cut(s) 1528, 1585
FauI CCCGC 1 cut(s) 1231
FblI GTMKAC 1 cut(s) 606
Fnu4HI GCNGC 9 cut(s) 27, 30, 33, 36, 39, 339, 342, 369, 813
FokI GGATG 2 cut(s) 1436, 1452
FriOI GRGCYC 5 cut(s) 213, 270, 809, 1089, 1528
Fsp4HI GCNGC 9 cut(s) 27, 30, 33, 36, 39, 339, 342, 369, 813
FspBI CTAG 2 cut(s) 1391, 1473
GluI GCNGC 9 cut(s) 27, 30, 33, 36, 39, 339, 342, 369, 813
HaeIII GGCC 8 cut(s) 695, 843, 927, 988, 1042, 1361, 1537, 1595
HapII CCGG 4 cut(s) 65, 563, 1274, 1362
Hin1II CATG 2 cut(s) 1532, 1589
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HinfI GANTC 7 cut(s) 107, 113, 590, 739, 758, 770, 1186
HpaII CCGG 4 cut(s) 65, 563, 1274, 1362
HphI GGTGA 5 cut(s) 79, 301, 721, 794, 1225
Hpy166II GTNNAC 2 cut(s) 607, 712
Hpy8I GTNNAC 2 cut(s) 607, 712
Hpy99I CGWCG 1 cut(s) 608
HpyCH4III ACNGT 2 cut(s) 7, 130
HpyCH4IV ACGT 1 cut(s) 902
HpyCH4V TGCA 4 cut(s) 745, 1067, 1093, 1157
HpyF10VI GCNNNNNNNGC 9 cut(s) 32, 35, 38, 377, 431, 458, 834, 1163, 1223
HpySE526I ACGT 1 cut(s) 902
Hsp92II CATG 2 cut(s) 1532, 1589
Kzo9I GATC 9 cut(s) 175, 653, 789, 799, 832, 1014, 1364, 1387, 1518
LguI GCTCTTC 2 cut(s) 131, 879
LmnI GCTCC 3 cut(s) 61, 1054, 1523
Lsp1109I GCAGC 4 cut(s) 325, 328, 355, 799
MaeI CTAG 2 cut(s) 1391, 1473
MaeII ACGT 1 cut(s) 902
MaeIII GTNAC 1 cut(s) 1231
MalI GATC 9 cut(s) 177, 655, 791, 801, 834, 1016, 1366, 1389, 1520
MboI GATC 9 cut(s) 175, 653, 789, 799, 832, 1014, 1364, 1387, 1518
MfeI CAATTG 1 cut(s) 950
MflI RGATCY 2 cut(s) 653, 789
MhlI GDGCHC 7 cut(s) 143, 213, 270, 678, 809, 1089, 1528
MlyI GAGTC 4 cut(s) 122, 733, 767, 1180
MroXI GAANNNNTTC 1 cut(s) 902
MseI TTAA 3 cut(s) 429, 456, 983
MspA1I CMGCKG 1 cut(s) 1262
MspI CCGG 4 cut(s) 65, 563, 1274, 1362
MspR9I CCNGG 6 cut(s) 20, 645, 697, 990, 1038, 1569
MunI CAATTG 1 cut(s) 950
MvaI CCWGG 6 cut(s) 20, 645, 697, 990, 1038, 1569
MvnI CGCG 1 cut(s) 837
MwoI GCNNNNNNNGC 9 cut(s) 32, 35, 38, 377, 431, 458, 834, 1163, 1223
NdeII GATC 9 cut(s) 175, 653, 789, 799, 832, 1014, 1364, 1387, 1518
NlaIII CATG 2 cut(s) 1532, 1589
NlaIV GGNNCC 3 cut(s) 63, 525, 1596
NmuCI GTSAC 1 cut(s) 1231
NruI TCGCGA 1 cut(s) 837
PagI TCATGA 1 cut(s) 1528
PceI AGGCCT 1 cut(s) 988
PciSI GCTCTTC 2 cut(s) 131, 879
PcsI WCGNNNNNNNCGW 1 cut(s) 720
PdmI GAANNNNTTC 1 cut(s) 902
PfeI GAWTC 3 cut(s) 107, 590, 770
PinAI ACCGGT 1 cut(s) 1273
PkrI GCNGC 9 cut(s) 28, 31, 34, 37, 40, 340, 343, 370, 814
PleI GAGTC 4 cut(s) 121, 733, 766, 1180
PpsI GAGTC 4 cut(s) 121, 733, 766, 1180
PsiI TTATAA 1 cut(s) 150
Psp124BI GAGCTC 3 cut(s) 213, 809, 1528
Psp6I CCWGG 6 cut(s) 18, 643, 695, 988, 1036, 1567
PspEI GGTNACC 1 cut(s) 1231
PspGI CCWGG 6 cut(s) 18, 643, 695, 988, 1036, 1567
PspN4I GGNNCC 3 cut(s) 63, 525, 1596
PspPI GGNCC 4 cut(s) 693, 1040, 1535, 1594
PstI CTGCAG 1 cut(s) 747
PstNI CAGNNNCTG 6 cut(s) 338, 368, 395, 422, 449, 476
PsuI RGATCY 2 cut(s) 653, 789
PvuII CAGCTG 1 cut(s) 1262
RruI TCGCGA 1 cut(s) 837
RsaI GTAC 3 cut(s) 818, 1151, 1253
RsaNI GTAC 3 cut(s) 817, 1150, 1252
SacI GAGCTC 3 cut(s) 213, 809, 1528
SalI GTCGAC 1 cut(s) 605
SapI GCTCTTC 2 cut(s) 131, 879
SaqAI TTAA 3 cut(s) 429, 456, 983
SatI GCNGC 9 cut(s) 27, 30, 33, 36, 39, 339, 342, 369, 813
Sau3AI GATC 9 cut(s) 175, 653, 789, 799, 832, 1014, 1364, 1387, 1518
Sau96I GGNCC 4 cut(s) 693, 1040, 1535, 1594
SchI GAGTC 4 cut(s) 122, 733, 767, 1180
ScrFI CCNGG 6 cut(s) 20, 645, 697, 990, 1038, 1569
SduI GDGCHC 7 cut(s) 143, 213, 270, 678, 809, 1089, 1528
SfcI CTRYAG 1 cut(s) 743
SmlI CTYRAG 6 cut(s) 179, 688, 934, 1025, 1247, 1355
SmoI CTYRAG 6 cut(s) 179, 688, 934, 1025, 1247, 1355
SseBI AGGCCT 1 cut(s) 988
SsiI CCGC 7 cut(s) 26, 29, 32, 35, 38, 296, 1224
SspI AATATT 1 cut(s) 1126
SspMI CTAG 2 cut(s) 1391, 1473
SstI GAGCTC 3 cut(s) 213, 809, 1528
StuI AGGCCT 1 cut(s) 988
StyD4I CCNGG 6 cut(s) 18, 643, 695, 988, 1036, 1567
TaaI ACNGT 2 cut(s) 7, 130
TaiI ACGT 1 cut(s) 905
TaqI TCGA 6 cut(s) 218, 225, 309, 606, 723, 761
TatI WGTACW 1 cut(s) 1251
TauI GCSGC 5 cut(s) 29, 32, 35, 38, 41
TfiI GAWTC 3 cut(s) 107, 590, 770
Tru1I TTAA 3 cut(s) 429, 456, 983
Tru9I TTAA 3 cut(s) 429, 456, 983
TscAI CASTG 2 cut(s) 133, 752
TseFI GTSAC 1 cut(s) 1231
TseI GCWGC 4 cut(s) 338, 341, 368, 812
Tsp45I GTSAC 1 cut(s) 1231
TspDTI ATGAA 4 cut(s) 927, 1120, 1494, 1545
TspRI CASTG 2 cut(s) 133, 752
XagI CCTNNNNNAGG 1 cut(s) 648
XapI RAATTY 1 cut(s) 683
XcmI CCANNNNNNNNNTGG 1 cut(s) 1605
XmiI GTMKAC 1 cut(s) 606
XmnI GAANNNNTTC 1 cut(s) 902
XspI CTAG 2 cut(s) 1391, 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.