Prupe.4G099600_v2.0.a1

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0001644
Physical Location & Seq
Reverse (-)
5076566 .. 5078831
2266 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G099600.2

Sequence Viewer

Length: 1551 bp
ATGACTGTTGAGGTTAAGGTTGAGGAGACCCTAGTGGCTGAAGTTGGGGTTGTTCCTCAGGAGGAGCCCATGAAGCCTGTGATTGAGAATGAGAAAGTTGTTGAAGGAGATGATAAGGTGAAGGAAGTGGCTGAGTCTGAATCCAAGCCCAGAGCAGTTGAGAAGAGCTCTTCTTATAAGGAAGAGAGCAACTTTCTCTCTGATCTGAAAGAGTTTGAGAGGAAGGCTTTGAGTGAGCTCAAATTGAAGCTGGAAGAAGCCATTTTAGGCAACAATATCTTCAAGAAAGAGGAGCCAAAGGAGAATGAGGAGAAGGAAAAAAAAGTAGCAGAGGAGGAAGAGAAGAAGGAAGAGAAGGAGGACGAGAAGAAGGACGAGAAAGCAGAGGAGGTTGATGAAAAAAAGACAGAGGAAGGAGATGAAGTACCAGTAGCTGAAGAGAAAGAAGCAGCAGCCCAAGAAGGTGAGGAAGAGAAGAAGCCTGAAGAGGGGTGCGAAGTAGACAAGGATATCTCTCTTTGGGGTGTGCCCCTTTTCCCTGGGAAAGGATTTGAAGGCACTGATGTGGTGCTCTTGAAGTTCTTGAGGGCTAGGGAATTCAAGGTCAATGAAGCCTTTGAGATGCTGAAGAAAACCCTTCAATGGAGGAAGGAGTCTAAGATTGATTCAATCTTGGATGAAGATATCTGTGCTGATCTGAGCTCTGCAGCTTACTTGAACGGCATTGATCGCGAAGGCCACCCTGTGTGCTACAACATTTTTGGGGTGTTTGATAACGAGGAGCTTTATCAGAAAACTTTTGGAAATGAGGAGAAGAGAGGGCAGTTCTTGAGGTGGAGGCTTCAGTTGATGGAGAAGAGCATTCAGAAGCTTGATTTGAGGCCTGGTGGTTCTTCTTCTTTGCTTCAAATCAATGACCTCAAGAACTCACCTGGACCTGTCAAGAAGGAGCTTAGAATTGCCACAAAGCAAGCTGTTGGGCTTCTGCAGGACAATTACCCTGAGTTAGTCGCCAAAAATATTTTCATAAATGTTCCATTCTGGTACTATGCACTCAATGCCCTATTATCTCCTTTCTTGACTCAAAGAACCAAGAGCAAGTTTGTGGTTGCTCGCCCAGCTAAGGTCACTGAGACCCTTCTCAAGTACATTCCAGCTCAGGAGATACCATCTCAATATGGTGGCTTCAAAAGGGACAATGACACTGAGTTCTCCTCTGAAGATTGTGCTGTTTCAGAACTAATTCTTAGGGCTGGATCCACTGGAACCATTCAGATAGATGCAGCTGAGGCTGATAACACATTAGTCTGGGACCTGACTGTTTTGGGTTGGGAAGTGAATTACAAGGAGGAGTTTGTTCCTACTGATGAGGGCTCTTACACCATTATTGTTCAGAAGAGAAAGAAAATAGGCTCCAATAATGAAGGGCCTATCCGCAACACTTTCAGAAGCAATGAACCAGGGAAGGTTGTTCTTACAATTGAGAACACTTCAAGCAAGAAGAAGAGGGTTCTGTACCGGTACAAGGCGAAGAAGTGCTCCACCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

517

Amino Acids

58.77

Weight (kDa)

4.97

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AccI GTMKAC 1 cut(s) 501
AccII CGCG 1 cut(s) 732
AciI CCGC 1 cut(s) 1435
AclWI GGATC 2 cut(s) 1251, 1264
AcsI RAATTY 1 cut(s) 596
AcuI CTGAAG 6 cut(s) 60, 456, 504, 647, 827, 1239
AdeI CACNNNGTG 1 cut(s) 745
AfaI GTAC 5 cut(s) 426, 1046, 1148, 1517, 1523
AfiI CCNNNNNNNGG 5 cut(s) 488, 545, 642, 1123, 1525
AgeI ACCGGT 1 cut(s) 1518
AjnI CCWGG 4 cut(s) 538, 883, 931, 1459
AjuI GAANNNNNNNTTGG 2 cut(s) 1007, 1039
AleI CACNNNNGTG 1 cut(s) 563
Alw21I GWGCWC 5 cut(s) 170, 240, 573, 704, 1541
Alw26I GTCTC 2 cut(s) 20, 1127
AlwI GGATC 2 cut(s) 1251, 1264
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 3 cut(s) 736, 881, 1427
ApeKI GCWGC 4 cut(s) 449, 452, 707, 1283
ApoI RAATTY 1 cut(s) 596
AsiGI ACCGGT 1 cut(s) 1518
Asp700I GAANNNNTTC 1 cut(s) 1242
AspS9I GGNCC 3 cut(s) 935, 1312, 1427
AsuHPI GGTGA 3 cut(s) 130, 476, 921
AvaII GGWCC 2 cut(s) 935, 1312
AxyI CCTNAGG 1 cut(s) 57
BaeGI GKGCMC 1 cut(s) 531
BamHI GGATCC 1 cut(s) 1256
BanII GRGCYC 5 cut(s) 69, 170, 240, 704, 1376
Bbv12I GWGCWC 5 cut(s) 170, 240, 573, 704, 1541
BbvCI CCTCAGC 1 cut(s) 1287
BbvI GCAGC 4 cut(s) 461, 464, 719, 1295
BccI CCATC 2 cut(s) 844, 1177
BceAI ACGGC 1 cut(s) 736
BciT130I CCWGG 4 cut(s) 540, 885, 933, 1461
BcoDI GTCTC 2 cut(s) 20, 1127
BfaI CTAG 2 cut(s) 32, 591
BfmI CTRYAG 2 cut(s) 705, 986
BisI GCNGC 4 cut(s) 450, 453, 708, 1284
BlsI GCNGC 4 cut(s) 451, 454, 709, 1285
Bme1390I CCNGG 4 cut(s) 540, 885, 933, 1461
Bme18I GGWCC 2 cut(s) 935, 1312
BmgT120I GGNCC 3 cut(s) 935, 1312, 1427
BmiI GGNNCC 6 cut(s) 66, 294, 1258, 1267, 1313, 1414
BmrFI CCNGG 4 cut(s) 540, 885, 933, 1461
BmsI GCATC 2 cut(s) 612, 1270
BplI GAGNNNNNCTC 4 cut(s) 152, 184, 1199, 1231
Bpu10I CCTNAGC 3 cut(s) 1122, 1158, 1287
BpuEI CTTGAG 4 cut(s) 604, 850, 905, 1127
BsaI GGTCTC 2 cut(s) 20, 1127
BsaJI CCNNGG 3 cut(s) 538, 539, 1460
BsaWI WCCGGW 1 cut(s) 1518
BsaXI ACNNNNNCTCC 2 cut(s) 408, 438
Bsc4I CCNNNNNNNGG 5 cut(s) 488, 545, 642, 1123, 1525
Bse118I RCCGGY 1 cut(s) 1518
Bse1I ACTGG 2 cut(s) 428, 1267
Bse21I CCTNAGG 1 cut(s) 57
Bse3DI GCAATG 1 cut(s) 1459
BseBI CCWGG 4 cut(s) 540, 885, 933, 1461
BseDI CCNNGG 3 cut(s) 538, 539, 1460
BseGI GGATG 1 cut(s) 682
BseLI CCNNNNNNNGG 5 cut(s) 488, 545, 642, 1123, 1525
BseMI GCAATG 1 cut(s) 1459
BseMII CTCAG 8 cut(s) 71, 123, 689, 993, 1122, 1172, 1197, 1278
BseNI ACTGG 2 cut(s) 428, 1267
BseSI GKGCMC 1 cut(s) 531
BseXI GCAGC 4 cut(s) 461, 464, 719, 1295
BseYI CCCAGC 1 cut(s) 1117
Bsh1236I CGCG 1 cut(s) 732
BshFI GGCC 3 cut(s) 738, 883, 1429
BshTI ACCGGT 1 cut(s) 1518
BsiHKAI GWGCWC 5 cut(s) 170, 240, 573, 704, 1541
BsiSI CCGG 1 cut(s) 1519
BslFI GGGAC 2 cut(s) 1208, 1325
BslI CCNNNNNNNGG 5 cut(s) 488, 545, 642, 1123, 1525
BsmAI GTCTC 2 cut(s) 20, 1127
BsmFI GGGAC 2 cut(s) 1208, 1325
BsmI GAATGC 1 cut(s) 861
BsnI GGCC 3 cut(s) 738, 883, 1429
Bso31I GGTCTC 2 cut(s) 20, 1127
Bsp1286I GDGCHC 8 cut(s) 69, 170, 240, 531, 573, 704, 1376, 1541
Bsp143I GATC 4 cut(s) 202, 694, 727, 1256
Bsp68I TCGCGA 1 cut(s) 732
BspACI CCGC 1 cut(s) 1435
BspANI GGCC 3 cut(s) 738, 883, 1429
BspCNI CTCAG 8 cut(s) 70, 124, 690, 994, 1123, 1171, 1198, 1279
BspFNI CGCG 1 cut(s) 732
BspLI GGNNCC 6 cut(s) 66, 294, 1258, 1267, 1313, 1414
BspMAI CTGCAG 2 cut(s) 709, 990
BspPI GGATC 2 cut(s) 1251, 1264
BspQI GCTCTTC 3 cut(s) 158, 175, 851
BspTNI GGTCTC 2 cut(s) 20, 1127
BsrDI GCAATG 1 cut(s) 1459
BsrFI RCCGGY 1 cut(s) 1518
BsrI ACTGG 2 cut(s) 428, 1267
BssAI RCCGGY 1 cut(s) 1518
BssECI CCNNGG 3 cut(s) 538, 539, 1460
BssMI GATC 4 cut(s) 202, 694, 727, 1256
Bst2UI CCWGG 4 cut(s) 540, 885, 933, 1461
Bst4CI ACNGT 2 cut(s) 7, 1321
BstAPI GCANNNNNTGC 1 cut(s) 1058
BstC8I GCNNGC 2 cut(s) 972, 1114
BstENI CCTNNNNNAGG 1 cut(s) 543
BstF5I GGATG 1 cut(s) 682
BstFNI CGCG 1 cut(s) 732
BstKTI GATC 4 cut(s) 205, 697, 730, 1259
BstMAI GTCTC 2 cut(s) 20, 1127
BstMBI GATC 4 cut(s) 202, 694, 727, 1256
BstMWI GCNNNNNNNGC 5 cut(s) 73, 729, 1058, 1118, 1289
BstNI CCWGG 4 cut(s) 540, 885, 933, 1461
BstSCI CCNGG 4 cut(s) 538, 883, 931, 1459
BstSFI CTRYAG 2 cut(s) 705, 986
BstSLI GKGCMC 1 cut(s) 531
BstUI CGCG 1 cut(s) 732
BstV1I GCAGC 4 cut(s) 461, 464, 719, 1295
BstX2I RGATCY 1 cut(s) 1256
BstYI RGATCY 1 cut(s) 1256
Bsu36I CCTNAGG 1 cut(s) 57
BsuRI GGCC 3 cut(s) 738, 883, 1429
BtsCI GGATG 1 cut(s) 682
BtsIMutI CAGTG 4 cut(s) 558, 1128, 1203, 1260
BtuMI TCGCGA 1 cut(s) 732
Cac8I GCNNGC 2 cut(s) 972, 1114
CaiI CAGNNNCTG 1 cut(s) 434
Cfr10I RCCGGY 1 cut(s) 1518
Cfr13I GGNCC 3 cut(s) 935, 1312, 1427
Csp6I GTAC 5 cut(s) 425, 1045, 1147, 1516, 1522
CspAI ACCGGT 1 cut(s) 1518
CviAII CATG 1 cut(s) 70
CviQI GTAC 5 cut(s) 425, 1045, 1147, 1516, 1522
DpnI GATC 4 cut(s) 204, 696, 729, 1258
DpnII GATC 4 cut(s) 202, 694, 727, 1256
DraIII CACNNNGTG 1 cut(s) 745
Ecl136II GAGCTC 3 cut(s) 168, 238, 702
Eco147I AGGCCT 1 cut(s) 883
Eco24I GRGCYC 5 cut(s) 69, 170, 240, 704, 1376
Eco31I GGTCTC 2 cut(s) 20, 1127
Eco32I GATATC 2 cut(s) 511, 685
Eco47I GGWCC 2 cut(s) 935, 1312
Eco53kI GAGCTC 3 cut(s) 168, 238, 702
Eco57I CTGAAG 6 cut(s) 60, 456, 504, 647, 827, 1239
Eco81I CCTNAGG 1 cut(s) 57
EcoICRI GAGCTC 3 cut(s) 168, 238, 702
EcoNI CCTNNNNNAGG 1 cut(s) 543
EcoO109I RGGNCCY 2 cut(s) 1312, 1427
EcoRI GAATTC 1 cut(s) 596
EcoRII CCWGG 4 cut(s) 538, 883, 931, 1459
EcoRV GATATC 2 cut(s) 511, 685
EcoT38I GRGCYC 5 cut(s) 69, 170, 240, 704, 1376
FaeI CATG 1 cut(s) 73
FaiI YATR 5 cut(s) 71, 177, 1028, 1050, 1179
FaqI GGGAC 2 cut(s) 1208, 1325
FatI CATG 1 cut(s) 69
FblI GTMKAC 1 cut(s) 501
Fnu4HI GCNGC 4 cut(s) 450, 453, 708, 1284
FokI GGATG 1 cut(s) 689
FriOI GRGCYC 5 cut(s) 69, 170, 240, 704, 1376
Fsp4HI GCNGC 4 cut(s) 450, 453, 708, 1284
FspBI CTAG 2 cut(s) 32, 591
GluI GCNGC 4 cut(s) 450, 453, 708, 1284
GsaI CCCAGC 1 cut(s) 1121
HaeIII GGCC 3 cut(s) 738, 883, 1429
HapII CCGG 1 cut(s) 1519
Hin1II CATG 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 869
HinfI GANTC 5 cut(s) 134, 140, 653, 665, 1081
HpaII CCGG 1 cut(s) 1519
HphI GGTGA 3 cut(s) 130, 476, 921
Hpy166II GTNNAC 1 cut(s) 502
Hpy8I GTNNAC 1 cut(s) 502
HpyCH4III ACNGT 2 cut(s) 7, 1321
HpyCH4V TGCA 4 cut(s) 707, 988, 1052, 1283
HpyF10VI GCNNNNNNNGC 5 cut(s) 73, 729, 1058, 1118, 1289
Hsp92II CATG 1 cut(s) 73
Kzo9I GATC 4 cut(s) 202, 694, 727, 1256
LguI GCTCTTC 3 cut(s) 158, 175, 851
LmnI GCTCC 6 cut(s) 64, 292, 781, 949, 1418, 1544
Lsp1109I GCAGC 4 cut(s) 461, 464, 719, 1295
LweI GCATC 2 cut(s) 612, 1270
MaeI CTAG 2 cut(s) 32, 591
MaeIII GTNAC 1 cut(s) 1126
MalI GATC 4 cut(s) 204, 696, 729, 1258
MboI GATC 4 cut(s) 202, 694, 727, 1256
MfeI CAATTG 1 cut(s) 1479
MflI RGATCY 1 cut(s) 1256
MhlI GDGCHC 8 cut(s) 69, 170, 240, 531, 573, 704, 1376, 1541
MluCI AATT 7 cut(s) 242, 596, 957, 994, 1242, 1339, 1479
MlyI GAGTC 3 cut(s) 143, 662, 1075
MroXI GAANNNNTTC 1 cut(s) 1242
MseI TTAA 1 cut(s) 15
MslI CAYNNNNRTG 1 cut(s) 563
MspA1I CMGCKG 1 cut(s) 1286
MspI CCGG 1 cut(s) 1519
MspR9I CCNGG 4 cut(s) 540, 885, 933, 1461
MunI CAATTG 1 cut(s) 1479
Mva1269I GAATGC 1 cut(s) 861
MvaI CCWGG 4 cut(s) 540, 885, 933, 1461
MvnI CGCG 1 cut(s) 732
MwoI GCNNNNNNNGC 5 cut(s) 73, 729, 1058, 1118, 1289
NdeII GATC 4 cut(s) 202, 694, 727, 1256
NlaIII CATG 1 cut(s) 73
NlaIV GGNNCC 6 cut(s) 66, 294, 1258, 1267, 1313, 1414
NmuCI GTSAC 1 cut(s) 1126
NruI TCGCGA 1 cut(s) 732
OliI CACNNNNGTG 1 cut(s) 563
PasI CCCWGGG 1 cut(s) 539
PceI AGGCCT 1 cut(s) 883
PciSI GCTCTTC 3 cut(s) 158, 175, 851
PctI GAATGC 1 cut(s) 861
PdmI GAANNNNTTC 1 cut(s) 1242
PfeI GAWTC 2 cut(s) 140, 665
PinAI ACCGGT 1 cut(s) 1518
PkrI GCNGC 4 cut(s) 451, 454, 709, 1285
PleI GAGTC 3 cut(s) 142, 661, 1075
PpsI GAGTC 3 cut(s) 142, 661, 1075
PpuMI RGGWCCY 1 cut(s) 1312
PsiI TTATAA 1 cut(s) 177
Psp124BI GAGCTC 3 cut(s) 170, 240, 704
Psp5II RGGWCCY 1 cut(s) 1312
Psp6I CCWGG 4 cut(s) 538, 883, 931, 1459
PspFI CCCAGC 1 cut(s) 1117
PspGI CCWGG 4 cut(s) 538, 883, 931, 1459
PspN4I GGNNCC 6 cut(s) 66, 294, 1258, 1267, 1313, 1414
PspPI GGNCC 3 cut(s) 935, 1312, 1427
PspPPI RGGWCCY 1 cut(s) 1312
PstI CTGCAG 2 cut(s) 709, 990
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 1 cut(s) 1256
PvuII CAGCTG 1 cut(s) 1286
RruI TCGCGA 1 cut(s) 732
RsaI GTAC 5 cut(s) 426, 1046, 1148, 1517, 1523
RsaNI GTAC 5 cut(s) 425, 1045, 1147, 1516, 1522
RseI CAYNNNNRTG 1 cut(s) 563
SacI GAGCTC 3 cut(s) 170, 240, 704
SapI GCTCTTC 3 cut(s) 158, 175, 851
SaqAI TTAA 1 cut(s) 15
SatI GCNGC 4 cut(s) 450, 453, 708, 1284
Sau3AI GATC 4 cut(s) 202, 694, 727, 1256
Sau96I GGNCC 3 cut(s) 935, 1312, 1427
SchI GAGTC 3 cut(s) 143, 662, 1075
ScrFI CCNGG 4 cut(s) 540, 885, 933, 1461
SduI GDGCHC 8 cut(s) 69, 170, 240, 531, 573, 704, 1376, 1541
SfaNI GCATC 2 cut(s) 612, 1270
SfcI CTRYAG 2 cut(s) 705, 986
SinI GGWCC 2 cut(s) 935, 1312
SmiMI CAYNNNNRTG 1 cut(s) 563
SmlI CTYRAG 4 cut(s) 583, 829, 920, 1142
SmoI CTYRAG 4 cut(s) 583, 829, 920, 1142
Sse9I AATT 7 cut(s) 242, 596, 957, 994, 1242, 1339, 1479
SseBI AGGCCT 1 cut(s) 883
SsiI CCGC 1 cut(s) 1435
SspI AATATT 1 cut(s) 1021
SspMI CTAG 2 cut(s) 32, 591
SstI GAGCTC 3 cut(s) 170, 240, 704
StuI AGGCCT 1 cut(s) 883
StyD4I CCNGG 4 cut(s) 538, 883, 931, 1459
TaaI ACNGT 2 cut(s) 7, 1321
TasI AATT 7 cut(s) 242, 596, 957, 994, 1242, 1339, 1479
TatI WGTACW 1 cut(s) 1146
TfiI GAWTC 2 cut(s) 140, 665
Tru1I TTAA 1 cut(s) 15
Tru9I TTAA 1 cut(s) 15
TscAI CASTG 4 cut(s) 565, 1135, 1210, 1267
TseFI GTSAC 1 cut(s) 1126
TseI GCWGC 4 cut(s) 449, 452, 707, 1283
Tsp45I GTSAC 1 cut(s) 1126
TspDTI ATGAA 8 cut(s) 86, 411, 435, 624, 693, 1015, 1437, 1470
TspRI CASTG 4 cut(s) 565, 1135, 1210, 1267
VpaK11BI GGWCC 2 cut(s) 935, 1312
XagI CCTNNNNNAGG 1 cut(s) 543
XapI RAATTY 1 cut(s) 596
XmiI GTMKAC 1 cut(s) 501
XmnI GAANNNNTTC 1 cut(s) 1242
XspI CTAG 2 cut(s) 32, 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.