MD05G1265900.v1.1

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001644
Physical Location & Seq
Forward (+)
40058871 .. 40061161
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1265900.v1.1.491

Sequence Viewer

Length: 1551 bp
ATGACTGTGGAGGAGACCCAGGTTGCGGAAGTTGTGGTTGTTCCTCGGGAGGAGGCTGAGAAGGTTGTTTGTGAGAATGAGAAGGTTGGTGAGGGAGATGATAAGGTGAAGGAAGTGGAGGCGGCGGAATCGAAGCCGAAAACGGTTGAGAAGAGTTCTTCTTATAAGGAAGAGAGCAACTATCTCTCTGATTTGAAAGAGTTCGAGAAGAAGGCGTTGAGCGAGCTGAAATCAAAGCTGGAGGAGGCCATTTTGGGCAACAATATCTTCAAGAAGGAGGAGCCAAAGAAGGAAGAAAAAAAAGTAGCAGAGGAGGTAGTAAAAACGGAGGAGGTAGAAAAAACAGAGGAGGAAGTGAAAACACAGGAGGAGGAGGAGGAGGAGAAGGAAGAGAAACCACAGGAGGTATTTGATAAAAAGGCGGAGGAAGTAGCTGGAGAGAATGAGAAGGAAGCGGAAGAAGGCGAGGAGGTGAAGAAATCGGAAGATGGCTGTGAAGTAGTAGACAAGGATATCTCTCTTTGGGGAGTGCCACTTTTTCCCGGTAAGGGTTTCGAGGGCACTGATGTTGTTCTCTTGAAGTTCTTAAGGGCTAGGGAGTTCAAGGTCAATGAGGCTTTCGAGATGCTGAAGAAAACCCTTCAATGGCGGAAGGAGTCCAAGATCGATTCGATTTTGGACGAGGATGTCTGTGCCGATCTGAGCTCTGCCGCTTACTTGAACGGCGTTGATCGCGAAGGCCACCCCGTTTGCTACAACATTTTCGGGGTATTCGACAACGAGGAGCTTTATCAGAAGACTTTTGGAAATGAGGAGAAGAGAGGGCAGTTCCTGAGGTGGAGGCTCCAGCTGATGGAGAAGAGCGTACAGAAGCTTGATCTGAGGCCTGGTGGTGTCACTTCTCTGCTTCAGATCAACGACCTCAAGAACTCTCCGGGGCCGGTGAAGAAGGAGCTCCGGATTGCCACAAAGCAAGCAGTTGGGCTTCTGCAGGACAACTACCCTGAGTTGGTTGCCAAAAATATCTTCATAAATGTTCCTTTCTGGTATTATGCACTTAATGCATTGCTATCTCCTTTCTTGACTCAAAGAACCAAGAGCAAGTTCGTCGTTGCTCGCCCCACGAAGGTCACTGAAACCCTTCTCAAGTACATTCCAGCTCAGGAGATACCTGCTCAGTATGGCGGTTTCAAGAGGGACAACGACAACGAGTTCTCTGCCGAAGATGGCGCTGTTTCAGAACTTATTCTTACGGCTGGATCCACTGGAACCATACAAATTGATGCAGCAGAGGTTGGTAGCACAATACTGTGGGACTTGACTGTTTTGGGATGGGAAGTGAATTACAAGGAGGAGTTTGTTCCCACTGAGGAGGGGTCATACACCATTATTGTTCAGAAGAAGAAGAAAATGGGGTCTAATGAAGGCCCGATTCGCAACACTTTCCGAAGCAACGAACCCGGGAAGGTTGTCCTGACAATCGAGAACAAATCGAGCAAGAAGAAGAGGGTTCTGTACCGGTACAAGGCCAAGAAGTGCTCCACCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

517

Amino Acids

58.63

Weight (kDa)

4.99

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 157 - 212 1.2e-07 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 237 - 396 1.3e-23 CRAL/TRIO domain
GOLD_PATL1_C PF25099 419 - 502 9.7e-36 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 165
AasI GACNNNNNNGTC 1 cut(s) 686
Acc36I ACCTGC 1 cut(s) 1180
AccB7I CCANNNNNTGG 1 cut(s) 853
AccI GTMKAC 1 cut(s) 504
AccII CGCG 1 cut(s) 735
AccIII TCCGGA 1 cut(s) 957
AciI CCGC 8 cut(s) 26, 122, 125, 422, 455, 649, 711, 1185
AclWI GGATC 2 cut(s) 1254, 1267
AcuI CTGAAG 2 cut(s) 650, 893
AfaI GTAC 4 cut(s) 867, 1151, 1517, 1523
AfiI CCNNNNNNNGG 7 cut(s) 25, 548, 645, 853, 1009, 1126, 1525
AflII CTTAAG 1 cut(s) 586
AgeI ACCGGT 1 cut(s) 1518
AgsI TTSAA 7 cut(s) 196, 271, 580, 604, 644, 721, 1192
AjnI CCWGG 2 cut(s) 18, 886
AjuI GAANNNNNNNTTGG 4 cut(s) 1010, 1042, 1088, 1120
AluBI AGCT 9 cut(s) 226, 238, 434, 705, 787, 850, 874, 955, 1160
AluI AGCT 9 cut(s) 226, 238, 434, 705, 787, 850, 874, 955, 1160
Alw21I GWGCWC 3 cut(s) 707, 957, 1541
Alw26I GTCTC 1 cut(s) 8
AlwI GGATC 2 cut(s) 1254, 1267
AlwNI CAGNNNCTG 1 cut(s) 832
Ama87I CYCGRG 2 cut(s) 45, 1460
Aor13HI TCCGGA 1 cut(s) 957
AoxI GGCC 6 cut(s) 246, 739, 884, 938, 1426, 1527
ApeKI GCWGC 1 cut(s) 1286
AsiGI ACCGGT 1 cut(s) 1518
Asp700I GAANNNNTTC 3 cut(s) 200, 1140, 1245
AspLEI GCGC 1 cut(s) 1232
AspS9I GGNCC 2 cut(s) 938, 1427
AsuC2I CCSGG 4 cut(s) 543, 936, 1461, 1462
AsuHPI GGTGA 4 cut(s) 101, 118, 484, 955
AvaI CYCGRG 2 cut(s) 45, 1460
AxyI CCTNAGG 1 cut(s) 833
BaeGI GKGCMC 1 cut(s) 563
BamHI GGATCC 1 cut(s) 1259
BanII GRGCYC 2 cut(s) 707, 957
BbsI GAAGAC 1 cut(s) 803
Bbv12I GWGCWC 3 cut(s) 707, 957, 1541
BbvI GCAGC 1 cut(s) 1298
BccI CCATC 4 cut(s) 482, 847, 1220, 1326
BceAI ACGGC 2 cut(s) 739, 1269
BcgI CGANNNNNNTGC 4 cut(s) 1090, 1124, 1199, 1233
BciT130I CCWGG 2 cut(s) 20, 888
BcnI CCSGG 4 cut(s) 543, 936, 1461, 1462
BcoDI GTCTC 1 cut(s) 8
BfaI CTAG 1 cut(s) 594
BfmI CTRYAG 1 cut(s) 989
BfoI RGCGCY 1 cut(s) 1233
BfrI CTTAAG 1 cut(s) 586
BfuAI ACCTGC 1 cut(s) 1180
BisI GCNGC 3 cut(s) 123, 711, 1287
BlsI GCNGC 3 cut(s) 124, 712, 1288
Bme1390I CCNGG 6 cut(s) 20, 543, 888, 936, 1461, 1462
BmeT110I CYCGRG 2 cut(s) 45, 1460
BmgT120I GGNCC 2 cut(s) 938, 1427
BmiI GGNNCC 5 cut(s) 282, 845, 939, 1261, 1270
BmrFI CCNGG 6 cut(s) 20, 543, 888, 936, 1461, 1462
BmsI GCATC 2 cut(s) 615, 1273
BpiI GAAGAC 1 cut(s) 803
BpmI CTGGAG 3 cut(s) 260, 456, 830
Bpu10I CCTNAGC 1 cut(s) 1161
BpuEI CTTGAG 2 cut(s) 908, 1130
BpuMI CCSGG 4 cut(s) 543, 936, 1461, 1462
Bsa29I ATCGAT 1 cut(s) 666
BsaI GGTCTC 1 cut(s) 8
BsaJI CCNNGG 4 cut(s) 18, 44, 935, 1460
BsaWI WCCGGW 2 cut(s) 957, 1518
Bsc4I CCNNNNNNNGG 7 cut(s) 25, 548, 645, 853, 1009, 1126, 1525
Bse118I RCCGGY 2 cut(s) 940, 1518
Bse1I ACTGG 1 cut(s) 1270
Bse21I CCTNAGG 1 cut(s) 833
Bse3DI GCAATG 1 cut(s) 1064
BseAI TCCGGA 1 cut(s) 957
BseBI CCWGG 2 cut(s) 20, 888
BseCI ATCGAT 1 cut(s) 666
BseDI CCNNGG 4 cut(s) 18, 44, 935, 1460
BseGI GGATG 2 cut(s) 691, 1337
BseLI CCNNNNNNNGG 7 cut(s) 25, 548, 645, 853, 1009, 1126, 1525
BseMI GCAATG 1 cut(s) 1064
BseMII CTCAG 8 cut(s) 48, 692, 824, 872, 996, 1175, 1190, 1359
BseNI ACTGG 1 cut(s) 1270
BseSI GKGCMC 1 cut(s) 563
BseXI GCAGC 1 cut(s) 1298
Bsh1236I CGCG 1 cut(s) 735
BshFI GGCC 6 cut(s) 248, 741, 886, 940, 1428, 1529
BshTI ACCGGT 1 cut(s) 1518
BshVI ATCGAT 1 cut(s) 666
BsiHKAI GWGCWC 3 cut(s) 707, 957, 1541
BsiHKCI CYCGRG 2 cut(s) 45, 1460
BsiSI CCGG 6 cut(s) 543, 935, 941, 958, 1461, 1519
BslFI GGGAC 2 cut(s) 1211, 1328
BslI CCNNNNNNNGG 7 cut(s) 25, 548, 645, 853, 1009, 1126, 1525
BsmAI GTCTC 1 cut(s) 8
BsmFI GGGAC 2 cut(s) 1211, 1328
BsnI GGCC 6 cut(s) 248, 741, 886, 940, 1428, 1529
Bso31I GGTCTC 1 cut(s) 8
BsoBI CYCGRG 2 cut(s) 45, 1460
Bsp1286I GDGCHC 4 cut(s) 563, 707, 957, 1541
Bsp13I TCCGGA 1 cut(s) 957
Bsp143I GATC 6 cut(s) 663, 697, 730, 877, 912, 1259
Bsp68I TCGCGA 1 cut(s) 735
BspACI CCGC 8 cut(s) 26, 122, 125, 422, 455, 649, 711, 1185
BspANI GGCC 6 cut(s) 248, 741, 886, 940, 1428, 1529
BspCNI CTCAG 8 cut(s) 49, 693, 825, 873, 997, 1174, 1189, 1360
BspDI ATCGAT 1 cut(s) 666
BspEI TCCGGA 1 cut(s) 957
BspFNI CGCG 1 cut(s) 735
BspLI GGNNCC 5 cut(s) 282, 845, 939, 1261, 1270
BspMAI CTGCAG 1 cut(s) 993
BspMI ACCTGC 1 cut(s) 1180
BspPI GGATC 2 cut(s) 1254, 1267
BspQI GCTCTTC 1 cut(s) 854
BspTI CTTAAG 1 cut(s) 586
BspTNI GGTCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 1064
BsrFI RCCGGY 2 cut(s) 940, 1518
BsrI ACTGG 1 cut(s) 1270
BssAI RCCGGY 2 cut(s) 940, 1518
BssECI CCNNGG 4 cut(s) 18, 44, 935, 1460
BssMI GATC 6 cut(s) 663, 697, 730, 877, 912, 1259
Bst2UI CCWGG 2 cut(s) 20, 888
Bst4CI ACNGT 4 cut(s) 7, 145, 1311, 1324
Bst6I CTCTTC 6 cut(s) 146, 165, 384, 812, 854, 1499
BstAFI CTTAAG 1 cut(s) 586
BstAPI GCANNNNNTGC 1 cut(s) 1061
BstC8I GCNNGC 3 cut(s) 224, 975, 1117
BstDEI CTNAG 8 cut(s) 57, 701, 833, 881, 1005, 1161, 1176, 1368
BstF5I GGATG 2 cut(s) 691, 1337
BstFNI CGCG 1 cut(s) 735
BstH2I RGCGCY 1 cut(s) 1233
BstHHI GCGC 1 cut(s) 1232
BstKTI GATC 6 cut(s) 666, 700, 733, 880, 915, 1262
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 6 cut(s) 663, 697, 730, 877, 912, 1259
BstMWI GCNNNNNNNGC 3 cut(s) 732, 1061, 1434
BstNI CCWGG 2 cut(s) 20, 888
BstSCI CCNGG 6 cut(s) 18, 541, 886, 934, 1459, 1460
BstSFI CTRYAG 1 cut(s) 989
BstSLI GKGCMC 1 cut(s) 563
BstUI CGCG 1 cut(s) 735
BstV1I GCAGC 1 cut(s) 1298
BstV2I GAAGAC 1 cut(s) 803
BstX2I RGATCY 1 cut(s) 1259
BstYI RGATCY 1 cut(s) 1259
Bsu15I ATCGAT 1 cut(s) 666
Bsu36I CCTNAGG 1 cut(s) 833
BsuRI GGCC 6 cut(s) 248, 741, 886, 940, 1428, 1529
BsuTUI ATCGAT 1 cut(s) 666
BtsCI GGATG 2 cut(s) 691, 1337
BtsIMutI CAGTG 4 cut(s) 561, 1131, 1263, 1365
BtuMI TCGCGA 1 cut(s) 735
BveI ACCTGC 1 cut(s) 1180
Cac8I GCNNGC 3 cut(s) 224, 975, 1117
CaiI CAGNNNCTG 1 cut(s) 832
CfoI GCGC 1 cut(s) 1232
Cfr10I RCCGGY 2 cut(s) 940, 1518
Cfr13I GGNCC 2 cut(s) 938, 1427
Cfr9I CCCGGG 1 cut(s) 1460
ClaI ATCGAT 1 cut(s) 666
Csp6I GTAC 4 cut(s) 866, 1150, 1516, 1522
CspAI ACCGGT 1 cut(s) 1518
CspCI CAANNNNNGTGG 2 cut(s) 731, 766
CviQI GTAC 4 cut(s) 866, 1150, 1516, 1522
DdeI CTNAG 8 cut(s) 57, 701, 833, 881, 1005, 1161, 1176, 1368
DpnI GATC 6 cut(s) 665, 699, 732, 879, 914, 1261
DpnII GATC 6 cut(s) 663, 697, 730, 877, 912, 1259
DrdI GACNNNNNNGTC 1 cut(s) 686
DseDI GACNNNNNNGTC 1 cut(s) 686
Eam1104I CTCTTC 6 cut(s) 146, 165, 384, 812, 854, 1499
EarI CTCTTC 6 cut(s) 146, 165, 384, 812, 854, 1499
EciI GGCGGA 3 cut(s) 140, 437, 664
Ecl136II GAGCTC 2 cut(s) 705, 955
Eco147I AGGCCT 1 cut(s) 886
Eco24I GRGCYC 2 cut(s) 707, 957
Eco31I GGTCTC 1 cut(s) 8
Eco32I GATATC 1 cut(s) 514
Eco53kI GAGCTC 2 cut(s) 705, 955
Eco57I CTGAAG 2 cut(s) 650, 893
Eco81I CCTNAGG 1 cut(s) 833
Eco88I CYCGRG 2 cut(s) 45, 1460
EcoICRI GAGCTC 2 cut(s) 705, 955
EcoRII CCWGG 2 cut(s) 18, 886
EcoRV GATATC 1 cut(s) 514
EcoT22I ATGCAT 1 cut(s) 1066
EcoT38I GRGCYC 2 cut(s) 707, 957
FaiI YATR 6 cut(s) 165, 1031, 1053, 1182, 1274, 1381
FaqI GGGAC 2 cut(s) 1211, 1328
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 3 cut(s) 123, 711, 1287
FokI GGATG 2 cut(s) 698, 1344
FriOI GRGCYC 2 cut(s) 707, 957
Fsp4HI GCNGC 3 cut(s) 123, 711, 1287
FspBI CTAG 1 cut(s) 594
GlaI GCGC 1 cut(s) 1231
GluI GCNGC 3 cut(s) 123, 711, 1287
GsuI CTGGAG 3 cut(s) 260, 456, 830
HaeII RGCGCY 1 cut(s) 1233
HaeIII GGCC 6 cut(s) 248, 741, 886, 940, 1428, 1529
HapII CCGG 6 cut(s) 543, 935, 941, 958, 1461, 1519
HhaI GCGC 1 cut(s) 1232
Hin6I GCGC 1 cut(s) 1230
HinP1I GCGC 1 cut(s) 1230
HindIII AAGCTT 1 cut(s) 872
HinfI GANTC 5 cut(s) 128, 656, 668, 1084, 1432
HpaII CCGG 6 cut(s) 543, 935, 941, 958, 1461, 1519
HphI GGTGA 4 cut(s) 101, 118, 484, 955
Hpy166II GTNNAC 1 cut(s) 505
Hpy8I GTNNAC 1 cut(s) 505
Hpy99I CGWCG 1 cut(s) 1112
HpyCH4III ACNGT 4 cut(s) 7, 145, 1311, 1324
HpyCH4V TGCA 4 cut(s) 991, 1055, 1064, 1286
HpyF10VI GCNNNNNNNGC 3 cut(s) 732, 1061, 1434
HpyF3I CTNAG 8 cut(s) 57, 701, 833, 881, 1005, 1161, 1176, 1368
HspAI GCGC 1 cut(s) 1230
Kpn2I TCCGGA 1 cut(s) 957
Kzo9I GATC 6 cut(s) 663, 697, 730, 877, 912, 1259
LguI GCTCTTC 1 cut(s) 854
LmnI GCTCC 6 cut(s) 280, 784, 849, 952, 960, 1544
Lsp1109I GCAGC 1 cut(s) 1298
LweI GCATC 2 cut(s) 615, 1273
MaeI CTAG 1 cut(s) 594
MaeIII GTNAC 2 cut(s) 895, 1129
MalI GATC 6 cut(s) 665, 699, 732, 879, 914, 1261
MboI GATC 6 cut(s) 663, 697, 730, 877, 912, 1259
MflI RGATCY 1 cut(s) 1259
MhlI GDGCHC 4 cut(s) 563, 707, 957, 1541
MluCI AATT 2 cut(s) 1278, 1342
MlyI GAGTC 2 cut(s) 665, 1078
Mph1103I ATGCAT 1 cut(s) 1066
MroI TCCGGA 1 cut(s) 957
MroXI GAANNNNTTC 3 cut(s) 200, 1140, 1245
MseI TTAA 2 cut(s) 587, 1059
MspA1I CMGCKG 1 cut(s) 850
MspCI CTTAAG 1 cut(s) 586
MspI CCGG 6 cut(s) 543, 935, 941, 958, 1461, 1519
MspR9I CCNGG 6 cut(s) 20, 543, 888, 936, 1461, 1462
MvaI CCWGG 2 cut(s) 20, 888
MvnI CGCG 1 cut(s) 735
MwoI GCNNNNNNNGC 3 cut(s) 732, 1061, 1434
NciI CCSGG 4 cut(s) 543, 936, 1461, 1462
NdeII GATC 6 cut(s) 663, 697, 730, 877, 912, 1259
NlaIV GGNNCC 5 cut(s) 282, 845, 939, 1261, 1270
NmuCI GTSAC 2 cut(s) 895, 1129
NruI TCGCGA 1 cut(s) 735
NsiI ATGCAT 1 cut(s) 1066
PceI AGGCCT 1 cut(s) 886
PciSI GCTCTTC 1 cut(s) 854
PcsI WCGNNNNNNNCGW 1 cut(s) 771
PdmI GAANNNNTTC 3 cut(s) 200, 1140, 1245
PfeI GAWTC 3 cut(s) 128, 668, 1432
PflMI CCANNNNNTGG 1 cut(s) 853
PinAI ACCGGT 1 cut(s) 1518
PkrI GCNGC 3 cut(s) 124, 712, 1288
PleI GAGTC 2 cut(s) 664, 1078
PpsI GAGTC 2 cut(s) 664, 1078
PsiI TTATAA 1 cut(s) 165
Psp124BI GAGCTC 2 cut(s) 707, 957
Psp6I CCWGG 2 cut(s) 18, 886
PspGI CCWGG 2 cut(s) 18, 886
PspN4I GGNNCC 5 cut(s) 282, 845, 939, 1261, 1270
PspPI GGNCC 2 cut(s) 938, 1427
PstI CTGCAG 1 cut(s) 993
PstNI CAGNNNCTG 1 cut(s) 832
PsuI RGATCY 1 cut(s) 1259
PvuII CAGCTG 1 cut(s) 850
RruI TCGCGA 1 cut(s) 735
RsaI GTAC 4 cut(s) 867, 1151, 1517, 1523
RsaNI GTAC 4 cut(s) 866, 1150, 1516, 1522
SacI GAGCTC 2 cut(s) 707, 957
SapI GCTCTTC 1 cut(s) 854
SaqAI TTAA 2 cut(s) 587, 1059
SatI GCNGC 3 cut(s) 123, 711, 1287
Sau3AI GATC 6 cut(s) 663, 697, 730, 877, 912, 1259
Sau96I GGNCC 2 cut(s) 938, 1427
SchI GAGTC 2 cut(s) 665, 1078
ScrFI CCNGG 6 cut(s) 20, 543, 888, 936, 1461, 1462
SduI GDGCHC 4 cut(s) 563, 707, 957, 1541
SfaNI GCATC 2 cut(s) 615, 1273
SfcI CTRYAG 1 cut(s) 989
SmaI CCCGGG 1 cut(s) 1462
SmlI CTYRAG 3 cut(s) 586, 923, 1145
SmoI CTYRAG 3 cut(s) 586, 923, 1145
Sse9I AATT 2 cut(s) 1278, 1342
SseBI AGGCCT 1 cut(s) 886
SsiI CCGC 8 cut(s) 26, 122, 125, 422, 455, 649, 711, 1185
SspMI CTAG 1 cut(s) 594
SstI GAGCTC 2 cut(s) 707, 957
StuI AGGCCT 1 cut(s) 886
StyD4I CCNGG 6 cut(s) 18, 541, 886, 934, 1459, 1460
TaaI ACNGT 4 cut(s) 7, 145, 1311, 1324
TaqI TCGA 9 cut(s) 131, 204, 555, 621, 666, 671, 774, 1482, 1493
TasI AATT 2 cut(s) 1278, 1342
TatI WGTACW 1 cut(s) 1149
TauI GCSGC 2 cut(s) 125, 713
TfiI GAWTC 3 cut(s) 128, 668, 1432
Tru1I TTAA 2 cut(s) 587, 1059
Tru9I TTAA 2 cut(s) 587, 1059
TscAI CASTG 4 cut(s) 568, 1138, 1270, 1372
TseFI GTSAC 2 cut(s) 895, 1129
TseI GCWGC 1 cut(s) 1286
Tsp45I GTSAC 2 cut(s) 895, 1129
TspDTI ATGAA 2 cut(s) 1018, 1437
TspGWI ACGGA 1 cut(s) 341
TspMI CCCGGG 1 cut(s) 1460
TspRI CASTG 4 cut(s) 568, 1138, 1270, 1372
Van91I CCANNNNNTGG 1 cut(s) 853
Vha464I CTTAAG 1 cut(s) 586
XmaI CCCGGG 1 cut(s) 1460
XmiI GTMKAC 1 cut(s) 504
XmnI GAANNNNTTC 3 cut(s) 200, 1140, 1245
XspI CTAG 1 cut(s) 594
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.