RchiOBHm_Chr5g0018181

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001644
Physical Location & Seq
Forward (+)
12701677 .. 12704041
2365 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29840

Sequence Viewer

Length: 1644 bp
ATGACTGTGGAGGAGACCCAGGTGGCGGTGGCGGTGGCGGCTGAGGTCGTGGAGGTTGAGGAGCCGGTGAAGGAGTGCGAGAAGGCTGTTGTTGAGGAATCCGAGTCCAAGCCAACCACTGTTGAGAAGAGCACTTCTTATAAGGAGGAGAGCAACTATCTCTCTGATCTCAAGGAGTTTGAGAAGAAGGCTTTGGTTGAGCTCAAATCGAAGCTCGAAGAAGCCATTTTGGGCAACAACATTTTCAAGAAAGATGAGCCCAAGAAGGTTGAGGAGAAGGTGAAGGCCGTAGCTGACGTCGAGGAGAAGAAGGAAGAGAAAACAGAGGCTGCTGTGAAAACAACAGAGGAAACAGAGGCTGCTGTGAAAGCAGAGGAAACAGAGGCTGTTGTGAAAGCAGAGGAAACAGAGGCTGTTGTGAAAGCAGAGGAAACAGAGGCTGTTGTGAAAGCAGAGGAAACAGAGGCTGTTGTGAAAACAGAGTGTGCTGAAAAAACAGAGGAAGGAGAAAAGGCACCAGAATTGGCTGAGAAGAAAGAAGAATTAGCAGTTCCGGTATGCGAGGAAGAAGCGAAACCCGAATCCAGCGAAGTCGTCGACACAGATATCTCACTCTGGGGTGTGCCTCTGTTCCCTGCAAAAGGATCTGAGGGAACTGATGTGGTGCTCTTGAAATTCTTGAGGGCCAGGGAGTTCAAGGTGAACGAAGCCTTCGAGATGCTGAAGAAGACTCTGCAGTGGAGGAAGGAGTCGAAAATTGATTCAATTTTGGTTGAGGAGATCTGCTCTGATCTGAGCTCTGCTGCTTACTTGAACGGTGTGGATCGCGAAGGCCACCCTGTATGCTACAACATCTTCGGAGTGTTTGACAACGAAGAGCTTTACCAGAAGACGTTCGGAAATGAAGAGAAGAGAGGCCAATTCTTGAGGTGGAGGGTTCAATTGATGGAGAAGAGTATCCAGAAGCTTGAATTAAGGCCTGGTGGTGCTACTTCTCTGCTTCAGATCAATGACCTCAAGAACTCGCCTGGGCCAATCAAGAAGGAGCTTAGGGTTGCAACAAAGCAAGCTGTTGGGCTCTTGCAGGACAATTACCCTGAATTGGTTGCCAAAAATATTTTCATCAATGTTCCATTCTGGTACTATGCACTCAATGCTCTGTTGTCTCCATTCTTGACTCAAAGAACCAAGAGCAAGTTTGTGGTGGCTCGCCCCGCTAAGGTCACCGAGACCCTTCTCAAGTACATTCCAGCTGAGGAGATACCGGTTCAGTATGGTGGCTTCAAGAGGGAAAATGACACCGAGTTCTTCTCCTCCGAAGATGGTTCTGTTTCTGAGCTAACTCTCAAGGCCGGATCAACCCAAACCATAGAGATTGATGTAGCTGAGGTTGATAATAGCACATTGGTTTGGGATGTGAGTGTTTTGGGATGGGAAGTGAATTACAAGGAGGAGTTTGTTCCTAGTGATGAAGGGTCTTACACCATTATTGTTCAGAAGAAGAAGAAGATCGGAGCTACTGAAGGGCCTATTCGCAACACTTTCCGAAGCAATGAGCCTGGAAAGGTTGTCCTGACAATCGAGAACACTTCGAGCAAGAAGAAGAGGGTGCTGTACCGGCACAAGTCCAACAAGTGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

547

Amino Acids

61.44

Weight (kDa)

4.9

Isoelectric Point (pI)

51.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 193 - 243 4.6e-09 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 268 - 427 7.2e-24 CRAL/TRIO domain
GOLD_PATL1_C PF25099 450 - 535 1.2e-36 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 141
AatII GACGTC 1 cut(s) 300
AccB1I GGYRCC 1 cut(s) 514
AccI GTMKAC 1 cut(s) 597
AccII CGCG 1 cut(s) 828
AciI CCGC 4 cut(s) 26, 32, 38, 1215
AclWI GGATC 3 cut(s) 652, 831, 1363
AcsI RAATTY 1 cut(s) 674
AcuI CTGAAG 3 cut(s) 743, 986, 1542
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 3 cut(s) 1142, 1244, 1616
AfiI CCNNNNNNNGG 4 cut(s) 25, 641, 1102, 1219
AgeI ACCGGT 1 cut(s) 1264
AgsI TTSAA 8 cut(s) 247, 673, 697, 765, 814, 941, 971, 1285
AjnI CCWGG 5 cut(s) 18, 686, 979, 1027, 1558
AjuI GAANNNNNNNTTGG 4 cut(s) 176, 208, 1103, 1135
Alw21I GWGCWC 4 cut(s) 134, 204, 669, 800
Alw26I GTCTC 3 cut(s) 8, 1170, 1223
AlwI GGATC 3 cut(s) 652, 831, 1363
AlwNI CAGNNNCTG 6 cut(s) 329, 359, 386, 413, 440, 467
AoxI GGCC 8 cut(s) 285, 684, 832, 916, 977, 1031, 1350, 1526
ApeKI GCWGC 3 cut(s) 329, 359, 803
ApoI RAATTY 1 cut(s) 674
AsiGI ACCGGT 1 cut(s) 1264
Asp700I GAANNNNTTC 1 cut(s) 893
AspS9I GGNCC 3 cut(s) 684, 1031, 1526
AsuHPI GGTGA 4 cut(s) 79, 292, 712, 1216
BanI GGYRCC 1 cut(s) 514
BanII GRGCYC 4 cut(s) 204, 261, 800, 1080
BarI GAAGNNNNNNTAC 2 cut(s) 1265, 1297
BbsI GAAGAC 2 cut(s) 734, 896
Bbv12I GWGCWC 4 cut(s) 134, 204, 669, 800
BbvCI CCTCAGC 3 cut(s) 42, 1254, 1386
BbvI GCAGC 3 cut(s) 316, 346, 790
BccI CCATC 3 cut(s) 940, 1316, 1425
BceAI ACGGC 1 cut(s) 272
BciT130I CCWGG 5 cut(s) 20, 688, 981, 1029, 1560
BciVI GTATCC 1 cut(s) 968
BcoDI GTCTC 3 cut(s) 8, 1170, 1223
BfaI CTAG 1 cut(s) 1464
BfmI CTRYAG 1 cut(s) 734
BfuI GTATCC 1 cut(s) 968
BglII AGATCT 1 cut(s) 780
BisI GCNGC 4 cut(s) 39, 330, 360, 804
BlsI GCNGC 4 cut(s) 40, 331, 361, 805
Bme1390I CCNGG 5 cut(s) 20, 688, 981, 1029, 1560
BmgT120I GGNCC 3 cut(s) 684, 1031, 1526
BmiI GGNNCC 2 cut(s) 63, 516
BmrFI CCNGG 5 cut(s) 20, 688, 981, 1029, 1560
BmsI GCATC 1 cut(s) 708
BpiI GAAGAC 2 cut(s) 734, 896
BplI GAGNNNNNCTC 4 cut(s) 770, 802, 1295, 1327
Bpu10I CCTNAGC 5 cut(s) 42, 1049, 1218, 1254, 1386
BpuEI CTTGAG 6 cut(s) 155, 700, 946, 1001, 1223, 1331
BsaHI GRCGYC 1 cut(s) 297
BsaI GGTCTC 2 cut(s) 8, 1223
BsaJI CCNNGG 3 cut(s) 18, 687, 1028
BsaWI WCCGGW 2 cut(s) 553, 1264
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 641, 1102, 1219
Bse118I RCCGGY 3 cut(s) 64, 1264, 1617
Bse3DI GCAATG 1 cut(s) 1558
BseBI CCWGG 5 cut(s) 20, 688, 981, 1029, 1560
BseDI CCNNGG 3 cut(s) 18, 687, 1028
BseGI GGATG 2 cut(s) 1420, 1436
BseLI CCNNNNNNNGG 4 cut(s) 25, 641, 1102, 1219
BseMI GCAATG 1 cut(s) 1558
BseMII CTCAG 7 cut(s) 33, 519, 639, 785, 1245, 1326, 1377
BseRI GAGGAG 9 cut(s) 26, 74, 161, 287, 317, 791, 1271, 1303, 1466
BseXI GCAGC 3 cut(s) 316, 346, 790
Bsh1236I CGCG 1 cut(s) 828
BshFI GGCC 8 cut(s) 287, 686, 834, 918, 979, 1033, 1352, 1528
BshNI GGYRCC 1 cut(s) 514
BshTI ACCGGT 1 cut(s) 1264
BsiHKAI GWGCWC 4 cut(s) 134, 204, 669, 800
BsiSI CCGG 5 cut(s) 65, 554, 1265, 1353, 1618
BslI CCNNNNNNNGG 4 cut(s) 25, 641, 1102, 1219
BsmAI GTCTC 3 cut(s) 8, 1170, 1223
BsnI GGCC 8 cut(s) 287, 686, 834, 918, 979, 1033, 1352, 1528
Bso31I GGTCTC 2 cut(s) 8, 1223
Bsp1286I GDGCHC 6 cut(s) 134, 204, 261, 669, 800, 1080
Bsp143I GATC 8 cut(s) 166, 644, 780, 790, 823, 1005, 1355, 1509
Bsp68I TCGCGA 1 cut(s) 828
BspACI CCGC 4 cut(s) 26, 32, 38, 1215
BspANI GGCC 8 cut(s) 287, 686, 834, 918, 979, 1033, 1352, 1528
BspCNI CTCAG 7 cut(s) 34, 520, 640, 786, 1246, 1327, 1378
BspFNI CGCG 1 cut(s) 828
BspLI GGNNCC 2 cut(s) 63, 516
BspMAI CTGCAG 1 cut(s) 738
BspPI GGATC 3 cut(s) 652, 831, 1363
BspQI GCTCTTC 2 cut(s) 122, 870
BspT107I GGYRCC 1 cut(s) 514
BspTNI GGTCTC 2 cut(s) 8, 1223
BsrDI GCAATG 1 cut(s) 1558
BsrFI RCCGGY 3 cut(s) 64, 1264, 1617
BssAI RCCGGY 3 cut(s) 64, 1264, 1617
BssECI CCNNGG 3 cut(s) 18, 687, 1028
BssMI GATC 8 cut(s) 166, 644, 780, 790, 823, 1005, 1355, 1509
BssNI GRCGYC 1 cut(s) 297
Bst2UI CCWGG 5 cut(s) 20, 688, 981, 1029, 1560
Bst4CI ACNGT 3 cut(s) 7, 121, 818
Bst6I CTCTTC 7 cut(s) 122, 309, 870, 900, 905, 947, 1598
BstACI GRCGYC 1 cut(s) 297
BstAPI GCANNNNNTGC 1 cut(s) 1154
BstC8I GCNNGC 2 cut(s) 1068, 1210
BstDEI CTNAG 9 cut(s) 42, 528, 648, 794, 1049, 1218, 1254, 1335, 1386
BstEII GGTNACC 1 cut(s) 1222
BstENI CCTNNNNNAGG 1 cut(s) 639
BstF5I GGATG 2 cut(s) 1420, 1436
BstFNI CGCG 1 cut(s) 828
BstKTI GATC 8 cut(s) 169, 647, 783, 793, 826, 1008, 1358, 1512
BstMAI GTCTC 3 cut(s) 8, 1170, 1223
BstMBI GATC 8 cut(s) 166, 644, 780, 790, 823, 1005, 1355, 1509
BstMWI GCNNNNNNNGC 5 cut(s) 38, 368, 1154, 1214, 1618
BstNI CCWGG 5 cut(s) 20, 688, 981, 1029, 1560
BstPI GGTNACC 1 cut(s) 1222
BstSCI CCNGG 5 cut(s) 18, 686, 979, 1027, 1558
BstSFI CTRYAG 1 cut(s) 734
BstUI CGCG 1 cut(s) 828
BstV1I GCAGC 3 cut(s) 316, 346, 790
BstV2I GAAGAC 2 cut(s) 734, 896
BstX2I RGATCY 2 cut(s) 644, 780
BstYI RGATCY 2 cut(s) 644, 780
BsuI GTATCC 1 cut(s) 968
BsuRI GGCC 8 cut(s) 287, 686, 834, 918, 979, 1033, 1352, 1528
BtsCI GGATG 2 cut(s) 1420, 1436
BtsI GCAGTG 1 cut(s) 743
BtsIMutI CAGTG 2 cut(s) 117, 743
BtuMI TCGCGA 1 cut(s) 828
Cac8I GCNNGC 2 cut(s) 1068, 1210
CaiI CAGNNNCTG 6 cut(s) 329, 359, 386, 413, 440, 467
Cfr10I RCCGGY 3 cut(s) 64, 1264, 1617
Cfr13I GGNCC 3 cut(s) 684, 1031, 1526
Csp6I GTAC 3 cut(s) 1141, 1243, 1615
CspAI ACCGGT 1 cut(s) 1264
CviQI GTAC 3 cut(s) 1141, 1243, 1615
DdeI CTNAG 9 cut(s) 42, 528, 648, 794, 1049, 1218, 1254, 1335, 1386
DpnI GATC 8 cut(s) 168, 646, 782, 792, 825, 1007, 1357, 1511
DpnII GATC 8 cut(s) 166, 644, 780, 790, 823, 1005, 1355, 1509
Eam1104I CTCTTC 7 cut(s) 122, 309, 870, 900, 905, 947, 1598
EarI CTCTTC 7 cut(s) 122, 309, 870, 900, 905, 947, 1598
Ecl136II GAGCTC 2 cut(s) 202, 798
Eco147I AGGCCT 1 cut(s) 979
Eco24I GRGCYC 4 cut(s) 204, 261, 800, 1080
Eco31I GGTCTC 2 cut(s) 8, 1223
Eco32I GATATC 1 cut(s) 607
Eco53kI GAGCTC 2 cut(s) 202, 798
Eco57I CTGAAG 3 cut(s) 743, 986, 1542
Eco91I GGTNACC 1 cut(s) 1222
EcoICRI GAGCTC 2 cut(s) 202, 798
EcoNI CCTNNNNNAGG 1 cut(s) 639
EcoO109I RGGNCCY 1 cut(s) 1526
EcoO65I GGTNACC 1 cut(s) 1222
EcoRII CCWGG 5 cut(s) 18, 686, 979, 1027, 1558
EcoRV GATATC 1 cut(s) 607
EcoT38I GRGCYC 4 cut(s) 204, 261, 800, 1080
FaiI YATR 6 cut(s) 141, 559, 844, 1146, 1275, 1370
FauI CCCGC 1 cut(s) 1222
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 4 cut(s) 39, 330, 360, 804
FokI GGATG 2 cut(s) 1427, 1443
FriOI GRGCYC 4 cut(s) 204, 261, 800, 1080
Fsp4HI GCNGC 4 cut(s) 39, 330, 360, 804
FspBI CTAG 1 cut(s) 1464
GluI GCNGC 4 cut(s) 39, 330, 360, 804
HaeIII GGCC 8 cut(s) 287, 686, 834, 918, 979, 1033, 1352, 1528
HapII CCGG 5 cut(s) 65, 554, 1265, 1353, 1618
Hin1I GRCGYC 1 cut(s) 297
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HindIII AAGCTT 1 cut(s) 965
HinfI GANTC 7 cut(s) 98, 104, 581, 730, 749, 761, 1177
HpaII CCGG 5 cut(s) 65, 554, 1265, 1353, 1618
HphI GGTGA 4 cut(s) 79, 292, 712, 1216
Hpy166II GTNNAC 2 cut(s) 598, 703
Hpy8I GTNNAC 2 cut(s) 598, 703
Hpy99I CGWCG 2 cut(s) 302, 599
HpyCH4III ACNGT 3 cut(s) 7, 121, 818
HpyCH4IV ACGT 2 cut(s) 297, 893
HpyCH4V TGCA 5 cut(s) 638, 736, 1058, 1084, 1148
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 368, 1154, 1214, 1618
HpyF3I CTNAG 9 cut(s) 42, 528, 648, 794, 1049, 1218, 1254, 1335, 1386
HpySE526I ACGT 2 cut(s) 297, 893
Hsp92I GRCGYC 1 cut(s) 297
Kzo9I GATC 8 cut(s) 166, 644, 780, 790, 823, 1005, 1355, 1509
LguI GCTCTTC 2 cut(s) 122, 870
LmnI GCTCC 3 cut(s) 61, 1045, 1514
Lsp1109I GCAGC 3 cut(s) 316, 346, 790
LweI GCATC 1 cut(s) 708
MaeI CTAG 1 cut(s) 1464
MaeII ACGT 2 cut(s) 297, 893
MaeIII GTNAC 1 cut(s) 1222
MalI GATC 8 cut(s) 168, 646, 782, 792, 825, 1007, 1357, 1511
MboI GATC 8 cut(s) 166, 644, 780, 790, 823, 1005, 1355, 1509
MfeI CAATTG 1 cut(s) 941
MflI RGATCY 2 cut(s) 644, 780
MhlI GDGCHC 6 cut(s) 134, 204, 261, 669, 800, 1080
MlyI GAGTC 4 cut(s) 113, 724, 758, 1171
MroXI GAANNNNTTC 1 cut(s) 893
MseI TTAA 1 cut(s) 974
MspA1I CMGCKG 1 cut(s) 1253
MspI CCGG 5 cut(s) 65, 554, 1265, 1353, 1618
MspR9I CCNGG 5 cut(s) 20, 688, 981, 1029, 1560
MunI CAATTG 1 cut(s) 941
MvaI CCWGG 5 cut(s) 20, 688, 981, 1029, 1560
MvnI CGCG 1 cut(s) 828
MwoI GCNNNNNNNGC 5 cut(s) 38, 368, 1154, 1214, 1618
NdeII GATC 8 cut(s) 166, 644, 780, 790, 823, 1005, 1355, 1509
NlaIV GGNNCC 2 cut(s) 63, 516
NmuCI GTSAC 1 cut(s) 1222
NruI TCGCGA 1 cut(s) 828
PceI AGGCCT 1 cut(s) 979
PciSI GCTCTTC 2 cut(s) 122, 870
PcsI WCGNNNNNNNCGW 1 cut(s) 711
PdmI GAANNNNTTC 1 cut(s) 893
PfeI GAWTC 3 cut(s) 98, 581, 761
PinAI ACCGGT 1 cut(s) 1264
PkrI GCNGC 4 cut(s) 40, 331, 361, 805
PleI GAGTC 4 cut(s) 112, 724, 757, 1171
PpsI GAGTC 4 cut(s) 112, 724, 757, 1171
PsiI TTATAA 1 cut(s) 141
Psp124BI GAGCTC 2 cut(s) 204, 800
Psp6I CCWGG 5 cut(s) 18, 686, 979, 1027, 1558
PspEI GGTNACC 1 cut(s) 1222
PspGI CCWGG 5 cut(s) 18, 686, 979, 1027, 1558
PspN4I GGNNCC 2 cut(s) 63, 516
PspPI GGNCC 3 cut(s) 684, 1031, 1526
PstI CTGCAG 1 cut(s) 738
PstNI CAGNNNCTG 6 cut(s) 329, 359, 386, 413, 440, 467
PsuI RGATCY 2 cut(s) 644, 780
PvuII CAGCTG 1 cut(s) 1253
RruI TCGCGA 1 cut(s) 828
RsaI GTAC 3 cut(s) 1142, 1244, 1616
RsaNI GTAC 3 cut(s) 1141, 1243, 1615
SacI GAGCTC 2 cut(s) 204, 800
SalI GTCGAC 1 cut(s) 596
SapI GCTCTTC 2 cut(s) 122, 870
SaqAI TTAA 1 cut(s) 974
SatI GCNGC 4 cut(s) 39, 330, 360, 804
Sau3AI GATC 8 cut(s) 166, 644, 780, 790, 823, 1005, 1355, 1509
Sau96I GGNCC 3 cut(s) 684, 1031, 1526
SchI GAGTC 4 cut(s) 113, 724, 758, 1171
ScrFI CCNGG 5 cut(s) 20, 688, 981, 1029, 1560
SduI GDGCHC 6 cut(s) 134, 204, 261, 669, 800, 1080
SfaNI GCATC 1 cut(s) 708
SfcI CTRYAG 1 cut(s) 734
SmlI CTYRAG 6 cut(s) 170, 679, 925, 1016, 1238, 1346
SmoI CTYRAG 6 cut(s) 170, 679, 925, 1016, 1238, 1346
SseBI AGGCCT 1 cut(s) 979
SsiI CCGC 4 cut(s) 26, 32, 38, 1215
SspI AATATT 1 cut(s) 1117
SspMI CTAG 1 cut(s) 1464
SstI GAGCTC 2 cut(s) 204, 800
StuI AGGCCT 1 cut(s) 979
StyD4I CCNGG 5 cut(s) 18, 686, 979, 1027, 1558
TaaI ACNGT 3 cut(s) 7, 121, 818
TaiI ACGT 2 cut(s) 300, 896
TaqI TCGA 8 cut(s) 209, 216, 300, 597, 714, 752, 1581, 1592
TatI WGTACW 1 cut(s) 1242
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 3 cut(s) 98, 581, 761
Tru1I TTAA 1 cut(s) 974
Tru9I TTAA 1 cut(s) 974
TscAI CASTG 2 cut(s) 124, 743
TseFI GTSAC 1 cut(s) 1222
TseI GCWGC 3 cut(s) 329, 359, 803
Tsp45I GTSAC 1 cut(s) 1222
TspDTI ATGAA 3 cut(s) 918, 1111, 1485
TspRI CASTG 2 cut(s) 124, 743
XagI CCTNNNNNAGG 1 cut(s) 639
XapI RAATTY 1 cut(s) 674
XmiI GTMKAC 1 cut(s) 597
XmnI GAANNNNTTC 1 cut(s) 893
XspI CTAG 1 cut(s) 1464
ZraI GACGTC 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.