MD05G1266100.v1.1

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001644
Physical Location & Seq
Forward (+)
40087059 .. 40088425
1367 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1266100.v1.1.491

Sequence Viewer

Length: 924 bp
ATGGTTGTGAAGGCTAGGGAGTTCAAGGTTAATGAGGCTTTTGAGATGCTGAAGAAAACCCTTCAATGGCGGAAGGAGTCCAAGATCGATTCGATTTTGGATGAGGATGTCTGTGCCGATCTGAGCTTTGCCGCTTACTTGAACGGCGTTGATCGCGAAGGCCACCCCGTTTGCTACAACATTTTTGGGGTTTTCGACAACGAGGAGCTCTATCAGATGACTTTTGGAAATGAGGAGAATGGAGGGCAATTCCTAAGGTGGAGGCTCCAGCTGATGGAGAAGAGCGTACAGAAGCTTGATCTGAGGCCTGGTGGTGTCACTTCTCTGCTTCAGATCAACGACCTCAAGAACTCTCCCGGTCCGGTGAAGAAGGAGCTCCGGATTGCCACAAAGCAAGCTGTTGGGCTTCTGCAGGACAACTACCATGAGTTGGTTGCCAAAAAGATCTTCATAAATGTTCCTTTCTGGTATTATGCACTTAATGCATTGCTATCTCCTTTCTTGACTCAAAGAACCAAGAGCAAGTTCGTCGTTGCTCGCCCCGCGAAGGTCACTGAAACCCTTCTCAAGTACATTCCAACTCAGGAGATACCTGCTCAGTACGGTGGTTTCAAGAGGGACAACGACAGCGAGTTTTCTGCCGAAGATGGCGCTGATTTATGTTTTGTTTGTAGCACATTACTGTGGGACTTGACTGTTTTAGGATGGGAAGTGAATTACAAGGAGGAATTTGTTCCAACTGATGAGGGGTCGTACACCATTATTGTTCAGAAGAAGAAGAAAATGGGGTCTAACGAAAGCCCAATTCGCAACACTCTCCGAAGAAACGAACCCGGGAAGGTTGTCCTGACAATCGAGAACAATTCGCGCAAGAAGAAGAAGGTTCTGTACCGGTACAAGGCCAAGAAGTGCTCCACCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

308

Amino Acids

35.29

Weight (kDa)

9.04

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 44 - 203 2.2e-22 CRAL/TRIO domain
GOLD_PATL1_C PF25099 225 - 293 2.1e-30 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 601
AccB7I CCANNNNNTGG 2 cut(s) 274, 430
AccII CGCG 3 cut(s) 156, 545, 868
AccIII TCCGGA 1 cut(s) 378
AciI CCGC 3 cut(s) 70, 132, 543
AcsI RAATTY 1 cut(s) 728
AcuI CTGAAG 2 cut(s) 71, 314
AfaI GTAC 6 cut(s) 288, 572, 602, 755, 890, 896
AfiI CCNNNNNNNGG 5 cut(s) 66, 274, 430, 547, 898
AgeI ACCGGT 1 cut(s) 891
AgsI TTSAA 4 cut(s) 25, 65, 142, 613
AjnI CCWGG 1 cut(s) 307
AjuI GAANNNNNNNTTGG 4 cut(s) 431, 463, 509, 541
AluBI AGCT 6 cut(s) 126, 208, 271, 295, 376, 398
AluI AGCT 6 cut(s) 126, 208, 271, 295, 376, 398
Alw21I GWGCWC 3 cut(s) 210, 378, 914
Ama87I CYCGRG 1 cut(s) 833
Aor13HI TCCGGA 1 cut(s) 378
AoxI GGCC 3 cut(s) 160, 305, 900
ApoI RAATTY 1 cut(s) 728
AsiGI ACCGGT 1 cut(s) 891
Asp700I GAANNNNTTC 2 cut(s) 561, 732
AspLEI GCGC 2 cut(s) 653, 870
AspS9I GGNCC 1 cut(s) 359
AsuC2I CCSGG 3 cut(s) 357, 834, 835
AsuHPI GGTGA 1 cut(s) 376
AvaI CYCGRG 1 cut(s) 833
AvaII GGWCC 1 cut(s) 359
AxyI CCTNAGG 1 cut(s) 254
BanII GRGCYC 2 cut(s) 210, 378
BarI GAAGNNNNNNTAC 2 cut(s) 872, 904
Bbv12I GWGCWC 3 cut(s) 210, 378, 914
BccI CCATC 3 cut(s) 268, 641, 699
BceAI ACGGC 1 cut(s) 160
BcgI CGANNNNNNTGC 4 cut(s) 511, 545, 620, 654
BciT130I CCWGG 1 cut(s) 309
BcnI CCSGG 3 cut(s) 357, 834, 835
BfaI CTAG 1 cut(s) 15
BfmI CTRYAG 1 cut(s) 410
BfoI RGCGCY 1 cut(s) 654
BfuAI ACCTGC 1 cut(s) 601
BglII AGATCT 1 cut(s) 444
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme1390I CCNGG 4 cut(s) 309, 357, 834, 835
Bme18I GGWCC 1 cut(s) 359
BmeT110I CYCGRG 1 cut(s) 833
BmgT120I GGNCC 1 cut(s) 359
BmiI GGNNCC 1 cut(s) 266
BmrFI CCNGG 4 cut(s) 309, 357, 834, 835
BmsI GCATC 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 251
BpuEI CTTGAG 2 cut(s) 329, 551
BpuMI CCSGG 3 cut(s) 357, 834, 835
Bsa29I ATCGAT 1 cut(s) 87
BsaJI CCNNGG 1 cut(s) 833
BsaWI WCCGGW 3 cut(s) 361, 378, 891
Bsc4I CCNNNNNNNGG 5 cut(s) 66, 274, 430, 547, 898
Bse118I RCCGGY 1 cut(s) 891
Bse21I CCTNAGG 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 485
BseAI TCCGGA 1 cut(s) 378
BseBI CCWGG 1 cut(s) 309
BseCI ATCGAT 1 cut(s) 87
BseDI CCNNGG 1 cut(s) 833
BseGI GGATG 3 cut(s) 106, 112, 710
BseLI CCNNNNNNNGG 5 cut(s) 66, 274, 430, 547, 898
BseMI GCAATG 1 cut(s) 485
BseMII CTCAG 4 cut(s) 113, 293, 596, 611
BseRI GAGGAG 2 cut(s) 218, 248
Bsh1236I CGCG 3 cut(s) 156, 545, 868
BshFI GGCC 3 cut(s) 162, 307, 902
BshTI ACCGGT 1 cut(s) 891
BshVI ATCGAT 1 cut(s) 87
BsiHKAI GWGCWC 3 cut(s) 210, 378, 914
BsiHKCI CYCGRG 1 cut(s) 833
BsiSI CCGG 5 cut(s) 357, 362, 379, 834, 892
BslFI GGGAC 2 cut(s) 632, 701
BslI CCNNNNNNNGG 5 cut(s) 66, 274, 430, 547, 898
BsmFI GGGAC 2 cut(s) 632, 701
BsnI GGCC 3 cut(s) 162, 307, 902
BsoBI CYCGRG 1 cut(s) 833
Bsp1286I GDGCHC 3 cut(s) 210, 378, 914
Bsp13I TCCGGA 1 cut(s) 378
Bsp143I GATC 6 cut(s) 84, 118, 151, 298, 333, 444
Bsp68I TCGCGA 1 cut(s) 156
BspACI CCGC 3 cut(s) 70, 132, 543
BspANI GGCC 3 cut(s) 162, 307, 902
BspCNI CTCAG 4 cut(s) 114, 294, 595, 610
BspDI ATCGAT 1 cut(s) 87
BspEI TCCGGA 1 cut(s) 378
BspFNI CGCG 3 cut(s) 156, 545, 868
BspLI GGNNCC 1 cut(s) 266
BspMAI CTGCAG 1 cut(s) 414
BspMI ACCTGC 1 cut(s) 601
BspQI GCTCTTC 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 485
BsrFI RCCGGY 1 cut(s) 891
BssAI RCCGGY 1 cut(s) 891
BssECI CCNNGG 1 cut(s) 833
BssMI GATC 6 cut(s) 84, 118, 151, 298, 333, 444
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 3 cut(s) 605, 684, 697
Bst6I CTCTTC 1 cut(s) 275
BstAPI GCANNNNNTGC 1 cut(s) 482
BstC8I GCNNGC 2 cut(s) 396, 538
BstDEI CTNAG 5 cut(s) 122, 254, 302, 582, 597
BstF5I GGATG 3 cut(s) 106, 112, 710
BstFNI CGCG 3 cut(s) 156, 545, 868
BstH2I RGCGCY 1 cut(s) 654
BstHHI GCGC 2 cut(s) 653, 870
BstKTI GATC 6 cut(s) 87, 121, 154, 301, 336, 447
BstMBI GATC 6 cut(s) 84, 118, 151, 298, 333, 444
BstMWI GCNNNNNNNGC 4 cut(s) 153, 482, 542, 807
BstNI CCWGG 1 cut(s) 309
BstSCI CCNGG 4 cut(s) 307, 355, 832, 833
BstSFI CTRYAG 1 cut(s) 410
BstUI CGCG 3 cut(s) 156, 545, 868
BstX2I RGATCY 1 cut(s) 444
BstYI RGATCY 1 cut(s) 444
Bsu15I ATCGAT 1 cut(s) 87
Bsu36I CCTNAGG 1 cut(s) 254
BsuRI GGCC 3 cut(s) 162, 307, 902
BsuTUI ATCGAT 1 cut(s) 87
BtsCI GGATG 3 cut(s) 106, 112, 710
BtsIMutI CAGTG 1 cut(s) 552
BtuMI TCGCGA 1 cut(s) 156
BveI ACCTGC 1 cut(s) 601
Cac8I GCNNGC 2 cut(s) 396, 538
CfoI GCGC 2 cut(s) 653, 870
Cfr10I RCCGGY 1 cut(s) 891
Cfr13I GGNCC 1 cut(s) 359
Cfr9I CCCGGG 1 cut(s) 833
ClaI ATCGAT 1 cut(s) 87
CpoI CGGWCCG 1 cut(s) 359
Csp6I GTAC 6 cut(s) 287, 571, 601, 754, 889, 895
CspAI ACCGGT 1 cut(s) 891
CspCI CAANNNNNGTGG 2 cut(s) 152, 187
CspI CGGWCCG 1 cut(s) 359
CviAII CATG 1 cut(s) 425
CviQI GTAC 6 cut(s) 287, 571, 601, 754, 889, 895
DdeI CTNAG 5 cut(s) 122, 254, 302, 582, 597
DpnI GATC 6 cut(s) 86, 120, 153, 300, 335, 446
DpnII GATC 6 cut(s) 84, 118, 151, 298, 333, 444
Eam1104I CTCTTC 1 cut(s) 275
EarI CTCTTC 1 cut(s) 275
EciI GGCGGA 1 cut(s) 85
Ecl136II GAGCTC 2 cut(s) 208, 376
Eco147I AGGCCT 1 cut(s) 307
Eco24I GRGCYC 2 cut(s) 210, 378
Eco47I GGWCC 1 cut(s) 359
Eco53kI GAGCTC 2 cut(s) 208, 376
Eco57I CTGAAG 2 cut(s) 71, 314
Eco81I CCTNAGG 1 cut(s) 254
Eco88I CYCGRG 1 cut(s) 833
EcoICRI GAGCTC 2 cut(s) 208, 376
EcoRII CCWGG 1 cut(s) 307
EcoT22I ATGCAT 1 cut(s) 487
EcoT38I GRGCYC 2 cut(s) 210, 378
FaeI CATG 1 cut(s) 428
FaiI YATR 5 cut(s) 426, 452, 474, 661, 922
FaqI GGGAC 2 cut(s) 632, 701
FatI CATG 1 cut(s) 424
FauI CCCGC 1 cut(s) 550
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 3 cut(s) 113, 119, 717
FriOI GRGCYC 2 cut(s) 210, 378
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 1 cut(s) 15
GlaI GCGC 2 cut(s) 652, 869
GluI GCNGC 1 cut(s) 132
GsuI CTGGAG 1 cut(s) 251
HaeII RGCGCY 1 cut(s) 654
HaeIII GGCC 3 cut(s) 162, 307, 902
HapII CCGG 5 cut(s) 357, 362, 379, 834, 892
HhaI GCGC 2 cut(s) 653, 870
Hin1II CATG 1 cut(s) 428
Hin6I GCGC 2 cut(s) 651, 868
HinP1I GCGC 2 cut(s) 651, 868
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 3 cut(s) 77, 89, 505
HpaII CCGG 5 cut(s) 357, 362, 379, 834, 892
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 1 cut(s) 756
Hpy188I TCNGA 6 cut(s) 123, 216, 303, 333, 771, 821
Hpy188III TCNNGA 8 cut(s) 155, 346, 379, 502, 584, 613, 847, 856
Hpy8I GTNNAC 1 cut(s) 756
Hpy99I CGWCG 1 cut(s) 533
HpyAV CCTTC 9 cut(s) 4, 67, 71, 152, 364, 541, 572, 832, 874
HpyCH4III ACNGT 3 cut(s) 605, 684, 697
HpyCH4V TGCA 3 cut(s) 412, 476, 485
HpyF10VI GCNNNNNNNGC 4 cut(s) 153, 482, 542, 807
HpyF3I CTNAG 5 cut(s) 122, 254, 302, 582, 597
Hsp92II CATG 1 cut(s) 428
HspAI GCGC 2 cut(s) 651, 868
Kpn2I TCCGGA 1 cut(s) 378
Kzo9I GATC 6 cut(s) 84, 118, 151, 298, 333, 444
LguI GCTCTTC 1 cut(s) 275
LmnI GCTCC 5 cut(s) 205, 270, 373, 381, 917
LweI GCATC 1 cut(s) 36
MaeI CTAG 1 cut(s) 15
MaeIII GTNAC 2 cut(s) 316, 550
MalI GATC 6 cut(s) 86, 120, 153, 300, 335, 446
MboI GATC 6 cut(s) 84, 118, 151, 298, 333, 444
MflI RGATCY 1 cut(s) 444
MhlI GDGCHC 3 cut(s) 210, 378, 914
MluCI AATT 5 cut(s) 248, 715, 728, 804, 862
MlyI GAGTC 2 cut(s) 86, 499
MmeI TCCRAC 2 cut(s) 602, 761
Mph1103I ATGCAT 1 cut(s) 487
MroI TCCGGA 1 cut(s) 378
MroXI GAANNNNTTC 2 cut(s) 561, 732
MseI TTAA 2 cut(s) 30, 480
MslI CAYNNNNRTG 1 cut(s) 682
MspA1I CMGCKG 1 cut(s) 271
MspI CCGG 5 cut(s) 357, 362, 379, 834, 892
MspR9I CCNGG 4 cut(s) 309, 357, 834, 835
MvaI CCWGG 1 cut(s) 309
MvnI CGCG 3 cut(s) 156, 545, 868
MwoI GCNNNNNNNGC 4 cut(s) 153, 482, 542, 807
NciI CCSGG 3 cut(s) 357, 834, 835
NdeII GATC 6 cut(s) 84, 118, 151, 298, 333, 444
NlaIII CATG 1 cut(s) 428
NlaIV GGNNCC 1 cut(s) 266
NmuCI GTSAC 2 cut(s) 316, 550
NruI TCGCGA 1 cut(s) 156
NsiI ATGCAT 1 cut(s) 487
PceI AGGCCT 1 cut(s) 307
PciSI GCTCTTC 1 cut(s) 275
PdmI GAANNNNTTC 2 cut(s) 561, 732
PfeI GAWTC 1 cut(s) 89
PflMI CCANNNNNTGG 2 cut(s) 274, 430
PinAI ACCGGT 1 cut(s) 891
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 2 cut(s) 85, 499
PpsI GAGTC 2 cut(s) 85, 499
Psp124BI GAGCTC 2 cut(s) 210, 378
Psp6I CCWGG 1 cut(s) 307
PspGI CCWGG 1 cut(s) 307
PspN4I GGNNCC 1 cut(s) 266
PspPI GGNCC 1 cut(s) 359
PstI CTGCAG 1 cut(s) 414
PsuI RGATCY 1 cut(s) 444
PvuII CAGCTG 1 cut(s) 271
RruI TCGCGA 1 cut(s) 156
RsaI GTAC 6 cut(s) 288, 572, 602, 755, 890, 896
RsaNI GTAC 6 cut(s) 287, 571, 601, 754, 889, 895
RseI CAYNNNNRTG 1 cut(s) 682
Rsr2I CGGWCCG 1 cut(s) 359
RsrII CGGWCCG 1 cut(s) 359
SacI GAGCTC 2 cut(s) 210, 378
SapI GCTCTTC 1 cut(s) 275
SaqAI TTAA 2 cut(s) 30, 480
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 6 cut(s) 84, 118, 151, 298, 333, 444
Sau96I GGNCC 1 cut(s) 359
SchI GAGTC 2 cut(s) 86, 499
ScrFI CCNGG 4 cut(s) 309, 357, 834, 835
SduI GDGCHC 3 cut(s) 210, 378, 914
SfaNI GCATC 1 cut(s) 36
SfcI CTRYAG 1 cut(s) 410
SinI GGWCC 1 cut(s) 359
SmaI CCCGGG 1 cut(s) 835
SmiMI CAYNNNNRTG 1 cut(s) 682
SmlI CTYRAG 2 cut(s) 344, 566
SmoI CTYRAG 2 cut(s) 344, 566
Sse9I AATT 5 cut(s) 248, 715, 728, 804, 862
SseBI AGGCCT 1 cut(s) 307
SsiI CCGC 3 cut(s) 70, 132, 543
SspMI CTAG 1 cut(s) 15
SstI GAGCTC 2 cut(s) 210, 378
StuI AGGCCT 1 cut(s) 307
StyD4I CCNGG 4 cut(s) 307, 355, 832, 833
TaaI ACNGT 3 cut(s) 605, 684, 697
TaqI TCGA 4 cut(s) 87, 92, 195, 855
TasI AATT 5 cut(s) 248, 715, 728, 804, 862
TatI WGTACW 1 cut(s) 570
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 2 cut(s) 30, 480
Tru9I TTAA 2 cut(s) 30, 480
TscAI CASTG 1 cut(s) 559
TseFI GTSAC 2 cut(s) 316, 550
Tsp45I GTSAC 2 cut(s) 316, 550
TspDTI ATGAA 1 cut(s) 439
TspMI CCCGGG 1 cut(s) 833
TspRI CASTG 1 cut(s) 559
Van91I CCANNNNNTGG 2 cut(s) 274, 430
VpaK11BI GGWCC 1 cut(s) 359
XapI RAATTY 1 cut(s) 728
XmaI CCCGGG 1 cut(s) 833
XmnI GAANNNNTTC 2 cut(s) 561, 732
XspI CTAG 1 cut(s) 15
Zsp2I ATGCAT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.