AT1G36070

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001698
Physical Location & Seq
Reverse (-)
13466694 .. 13470936
4243 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G36070.2

Sequence Viewer

Length: 1257 bp
ATGGAACAGACAAAGGATGATTCTGATTTTGAGGATGATTGTGAAACGAAGAAGATGGAGACTGATACATCAGCTCAAGAGGCAAGGAATGGTAAAGACATACAAGGGATTCAGTGGGAAGGGTTTAAGTATAATAGAGACGAGTTTCGTGAGACTAGGTTGAAACAATATGATAACTTTGTTAATATCTTAAGGCCTAGCTCTCGAGAAAAGCTTGATAAGGAACATGGGCAAGTAGTTGAGAAAGGAAAGAACTTTTATGACTTCCAATTCAATACAAGGCTTGTCACTTCCACTATTGTGCATTTTCAGCTTCGGAATTTGGTATGGGCGACATCTAAGCATGATGTATATCTAATGCAAAATTATTCACTCATGCACTGGTCATCTCTACTCCAGAGAGGGAAAGAAGTACTTAATGTCGCCAGATCAGTTACCCCCACTCAGAAACTACCTGGATTGTTTTCTGAGCCACTCTCTAGGGTGCAAGTTAGCAGCATGGCAGTCAAAGAAAATCTGATCCTTTTAGGAGGGTTCGACGGGGAGCTTCTCTGCAAGTGTGTAAATCAGCCTGGGGTTGCTTTTTGCACAAGATTATCAACGGAAGATAATGCCATCACAAACACAGTTGATATATACCGAGACCCAAGTGGCTCCCTTAGGCTTATAACCGCAAATAACGACTGTAAAATTCGTGTATTCGATGCTCAGAGCTTTACACGTGTCAGTGAATTTGCTTTAGATTGGTCTGTCAATAATACCTCAGTTAGCCCGGATGGGAAGCTACTCGCTGTAGTCGGGGACAGTACAGAATGCTTGATCTCTGATTCCCATTCTGGAAAAGTTATTTCAAGCCTCAGAGGCCACAAAGACTACTCATTTGCATCTGCTTGGCACCCGAATGGTCTAATCTTAGCAACGGGAAACCAAGACACGGCATGCCGTCTCTGGGACATTCGTAACCCGTCAGAATCATTCGCTGTCCTGAAAGGAAACATGGGAGCCATCAGAGGACTGAAGTTCACACCAGAAGGGCGGTTTCTTGCAATGGCTGAGCCTGCAGACTTTGTTCACATCTTCGACACGCAGTCCGGGTTTCTACAGTCCCAAGAGATTGATCTGTTTGGAGAAATAGCCGGGATCTCGTTCAGCCCCGATACAGAGGCACTATATGTAGGGGTCGCAGACCGCACTTATGGAAGTTTGCTGGAGTATAAGAGGAGAAAGGATAATCACTATATGGACTCCTTTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

418

Amino Acids

47.25

Weight (kDa)

5.49

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 160 - 355 7.9e-09 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 166 - 395 3.1e-20 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 206 - 306 1.9e-11 THOC3 beta-propeller domain
WD40_Gbeta PF25391 218 - 397 1.5e-09 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 220 - 398 2.1e-19 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 220 - 351 3e-12 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2 PF23414 221 - 364 3.7e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 229 - 397 1.2e-14 WDR3 first beta-propeller domain
WDR55 PF24796 251 - 392 6.3e-08 WDR55
WD40_CDC20-Fz PF24807 254 - 383 1.3e-12 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 284 - 396 1.6e-12 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 668
AccB1I GGYRCC 1 cut(s) 894
AciI CCGC 3 cut(s) 672, 1036, 1189
AclWI GGATC 2 cut(s) 514, 1148
AcsI RAATTY 3 cut(s) 319, 690, 731
AcuI CTGAAG 1 cut(s) 1037
AcvI CACGTG 1 cut(s) 722
AfaI GTAC 2 cut(s) 414, 808
AfiI CCNNNNNNNGG 2 cut(s) 934, 949
AflII CTTAAG 1 cut(s) 190
AflIII ACRYGT 2 cut(s) 719, 721
AgsI TTSAA 3 cut(s) 163, 274, 852
AhdI GACNNNNNGTC 1 cut(s) 1087
AjnI CCWGG 2 cut(s) 454, 571
AleI CACNNNNGTG 1 cut(s) 299
AluBI AGCT 7 cut(s) 74, 201, 214, 313, 547, 714, 784
AluI AGCT 7 cut(s) 74, 201, 214, 313, 547, 714, 784
Alw26I GTCTC 5 cut(s) 53, 132, 146, 636, 950
AlwI GGATC 2 cut(s) 514, 1148
Ama87I CYCGRG 1 cut(s) 204
AoxI GGCC 2 cut(s) 194, 862
ApeKI GCWGC 1 cut(s) 495
ApoI RAATTY 3 cut(s) 319, 690, 731
ArsI GACNNNNNNTTYG 2 cut(s) 863, 895
AsuC2I CCSGG 3 cut(s) 773, 1093, 1138
AvaI CYCGRG 1 cut(s) 204
AxyI CCTNAGG 1 cut(s) 659
BanI GGYRCC 1 cut(s) 894
BbrPI CACGTG 1 cut(s) 722
BbvI GCAGC 1 cut(s) 507
BccI CCATC 4 cut(s) 49, 623, 770, 1013
BceAI ACGGC 2 cut(s) 927, 951
BciT130I CCWGG 2 cut(s) 456, 573
BcnI CCSGG 3 cut(s) 773, 1093, 1138
BcoDI GTCTC 5 cut(s) 53, 132, 146, 636, 950
BfaI CTAG 3 cut(s) 156, 198, 480
BfmI CTRYAG 3 cut(s) 792, 1059, 1100
BfrI CTTAAG 1 cut(s) 190
BglI GCCNNNNNGGC 1 cut(s) 861
BisI GCNGC 1 cut(s) 496
BlpI GCTNAGC 1 cut(s) 1053
BlsI GCNGC 1 cut(s) 497
BmcAI AGTACT 1 cut(s) 414
Bme1390I CCNGG 5 cut(s) 456, 573, 773, 1093, 1138
BmeRI GACNNNNNGTC 1 cut(s) 1087
BmeT110I CYCGRG 1 cut(s) 204
BmiI GGNNCC 3 cut(s) 655, 896, 1003
BmrFI CCNGG 5 cut(s) 456, 573, 773, 1093, 1138
BmsI GCATC 2 cut(s) 694, 893
BplI GAGNNNNNCTC 2 cut(s) 461, 493
BpmI CTGGAG 2 cut(s) 380, 1229
Bpu1102I GCTNAGC 1 cut(s) 1053
BpuEI CTTGAG 1 cut(s) 60
BpuMI CCSGG 3 cut(s) 773, 1093, 1138
BsaAI YACGTR 1 cut(s) 722
BsaBI GATNNNNATC 1 cut(s) 351
BsaI GGTCTC 1 cut(s) 636
BsaJI CCNNGG 1 cut(s) 572
Bsc4I CCNNNNNNNGG 2 cut(s) 934, 949
Bse1I ACTGG 1 cut(s) 386
Bse21I CCTNAGG 1 cut(s) 659
Bse3DI GCAATG 1 cut(s) 1053
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 2 cut(s) 456, 573
BseDI CCNNGG 1 cut(s) 572
BseGI GGATG 3 cut(s) 22, 40, 781
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 2 cut(s) 934, 949
BseMI GCAATG 1 cut(s) 1053
BseMII CTCAG 6 cut(s) 458, 459, 722, 777, 871, 1044
BseNI ACTGG 1 cut(s) 386
BseRI GAGGAG 1 cut(s) 1234
BseXI GCAGC 1 cut(s) 507
BshFI GGCC 2 cut(s) 196, 864
BshNI GGYRCC 1 cut(s) 894
BsiHKCI CYCGRG 1 cut(s) 204
BsiSI CCGG 3 cut(s) 773, 1092, 1137
BslFI GGGAC 3 cut(s) 815, 965, 1090
BslI CCNNNNNNNGG 2 cut(s) 934, 949
BsmAI GTCTC 5 cut(s) 53, 132, 146, 636, 950
BsmBI CGTCTC 2 cut(s) 132, 950
BsmFI GGGAC 3 cut(s) 815, 965, 1090
BsmI GAATGC 1 cut(s) 818
BsnI GGCC 2 cut(s) 196, 864
Bso31I GGTCTC 1 cut(s) 636
BsoBI CYCGRG 1 cut(s) 204
Bsp143I GATC 5 cut(s) 428, 519, 819, 1117, 1140
Bsp1720I GCTNAGC 1 cut(s) 1053
BspACI CCGC 3 cut(s) 672, 1036, 1189
BspANI GGCC 2 cut(s) 196, 864
BspCNI CTCAG 6 cut(s) 457, 460, 721, 776, 870, 1045
BspLI GGNNCC 3 cut(s) 655, 896, 1003
BspMAI CTGCAG 1 cut(s) 1063
BspPI GGATC 2 cut(s) 514, 1148
BspT107I GGYRCC 1 cut(s) 894
BspTI CTTAAG 1 cut(s) 190
BspTNI GGTCTC 1 cut(s) 636
BsrDI GCAATG 1 cut(s) 1053
BsrI ACTGG 1 cut(s) 386
BssECI CCNNGG 1 cut(s) 572
BssMI GATC 5 cut(s) 428, 519, 819, 1117, 1140
Bst2UI CCWGG 2 cut(s) 456, 573
Bst4CI ACNGT 4 cut(s) 628, 686, 806, 1104
BstAFI CTTAAG 1 cut(s) 190
BstBAI YACGTR 1 cut(s) 722
BstC8I GCNNGC 2 cut(s) 940, 1059
BstDEI CTNAG 9 cut(s) 339, 444, 468, 659, 708, 763, 857, 913, 1053
BstF5I GGATG 3 cut(s) 22, 40, 781
BstKTI GATC 5 cut(s) 431, 522, 822, 1120, 1143
BstMAI GTCTC 5 cut(s) 53, 132, 146, 636, 950
BstMBI GATC 5 cut(s) 428, 519, 819, 1117, 1140
BstMWI GCNNNNNNNGC 4 cut(s) 80, 310, 861, 1058
BstNI CCWGG 2 cut(s) 456, 573
BstNSI RCATGY 1 cut(s) 942
BstSCI CCNGG 5 cut(s) 454, 571, 771, 1091, 1136
BstSFI CTRYAG 3 cut(s) 792, 1059, 1100
BstV1I GCAGC 1 cut(s) 507
BstX2I RGATCY 1 cut(s) 1140
BstYI RGATCY 1 cut(s) 1140
Bsu36I CCTNAGG 1 cut(s) 659
BsuRI GGCC 2 cut(s) 196, 864
BtsCI GGATG 3 cut(s) 22, 40, 781
BtsIMutI CAGTG 3 cut(s) 119, 379, 733
Cac8I GCNNGC 2 cut(s) 940, 1059
Csp6I GTAC 2 cut(s) 413, 807
CviAII CATG 6 cut(s) 227, 344, 376, 499, 939, 997
CviQI GTAC 2 cut(s) 413, 807
DdeI CTNAG 9 cut(s) 339, 444, 468, 659, 708, 763, 857, 913, 1053
DpnI GATC 5 cut(s) 430, 521, 821, 1119, 1142
DpnII GATC 5 cut(s) 428, 519, 819, 1117, 1140
DriI GACNNNNNGTC 1 cut(s) 1087
Eam1105I GACNNNNNGTC 1 cut(s) 1087
Eco147I AGGCCT 1 cut(s) 196
Eco31I GGTCTC 1 cut(s) 636
Eco57I CTGAAG 1 cut(s) 1037
Eco72I CACGTG 1 cut(s) 722
Eco81I CCTNAGG 1 cut(s) 659
Eco88I CYCGRG 1 cut(s) 204
EcoRII CCWGG 2 cut(s) 454, 571
Esp3I CGTCTC 2 cut(s) 132, 950
FaeI CATG 6 cut(s) 230, 347, 379, 502, 942, 1000
FalI AAGNNNNNCTT 2 cut(s) 399, 431
FaqI GGGAC 3 cut(s) 815, 965, 1090
FatI CATG 6 cut(s) 226, 343, 375, 498, 938, 996
Fnu4HI GCNGC 1 cut(s) 496
FokI GGATG 3 cut(s) 29, 47, 788
Fsp4HI GCNGC 1 cut(s) 496
FspBI CTAG 3 cut(s) 156, 198, 480
GluI GCNGC 1 cut(s) 496
GsuI CTGGAG 2 cut(s) 380, 1229
HaeIII GGCC 2 cut(s) 196, 864
HapII CCGG 3 cut(s) 773, 1092, 1137
Hin1II CATG 6 cut(s) 230, 347, 379, 502, 942, 1000
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 5 cut(s) 20, 109, 827, 971, 1244
HpaII CCGG 3 cut(s) 773, 1092, 1137
Hpy166II GTNNAC 2 cut(s) 1023, 1072
Hpy188III TCNNGA 7 cut(s) 77, 149, 204, 206, 397, 837, 985
Hpy8I GTNNAC 2 cut(s) 1023, 1072
Hpy99I CGWCG 1 cut(s) 542
HpyAV CCTTC 2 cut(s) 113, 1025
HpyCH4III ACNGT 4 cut(s) 628, 686, 806, 1104
HpyCH4IV ACGT 1 cut(s) 721
HpyCH4V TGCA 9 cut(s) 304, 361, 379, 487, 555, 588, 884, 1046, 1061
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 310, 861, 1058
HpyF3I CTNAG 9 cut(s) 339, 444, 468, 659, 708, 763, 857, 913, 1053
HpySE526I ACGT 1 cut(s) 721
Hsp92II CATG 6 cut(s) 230, 347, 379, 502, 942, 1000
Kzo9I GATC 5 cut(s) 428, 519, 819, 1117, 1140
LmnI GCTCC 3 cut(s) 544, 659, 1001
Lsp1109I GCAGC 1 cut(s) 507
LweI GCATC 2 cut(s) 694, 893
MaeI CTAG 3 cut(s) 156, 198, 480
MaeII ACGT 1 cut(s) 721
MaeIII GTNAC 3 cut(s) 286, 433, 959
MalI GATC 5 cut(s) 430, 521, 821, 1119, 1142
MboI GATC 5 cut(s) 428, 519, 819, 1117, 1140
MboII GAAGA 4 cut(s) 61, 64, 617, 1069
MflI RGATCY 1 cut(s) 1140
MluCI AATT 5 cut(s) 269, 319, 364, 690, 731
MlyI GAGTC 1 cut(s) 1238
MseI TTAA 4 cut(s) 126, 183, 191, 417
MslI CAYNNNNRTG 2 cut(s) 299, 900
MspCI CTTAAG 1 cut(s) 190
MspI CCGG 3 cut(s) 773, 1092, 1137
MspR9I CCNGG 5 cut(s) 456, 573, 773, 1093, 1138
Mva1269I GAATGC 1 cut(s) 818
MvaI CCWGG 2 cut(s) 456, 573
MwoI GCNNNNNNNGC 4 cut(s) 80, 310, 861, 1058
NciI CCSGG 3 cut(s) 773, 1093, 1138
NdeII GATC 5 cut(s) 428, 519, 819, 1117, 1140
NlaIII CATG 6 cut(s) 230, 347, 379, 502, 942, 1000
NlaIV GGNNCC 3 cut(s) 655, 896, 1003
NmuCI GTSAC 1 cut(s) 286
NspI RCATGY 1 cut(s) 942
OliI CACNNNNGTG 1 cut(s) 299
PaeI GCATGC 1 cut(s) 942
PaeR7I CTCGAG 1 cut(s) 204
PceI AGGCCT 1 cut(s) 196
PctI GAATGC 1 cut(s) 818
PfeI GAWTC 4 cut(s) 20, 109, 827, 971
PkrI GCNGC 1 cut(s) 497
PleI GAGTC 1 cut(s) 1238
PmaCI CACGTG 1 cut(s) 722
PmlI CACGTG 1 cut(s) 722
PpsI GAGTC 1 cut(s) 1238
Ppu21I YACGTR 1 cut(s) 722
PsiI TTATAA 1 cut(s) 668
Psp6I CCWGG 2 cut(s) 454, 571
PspCI CACGTG 1 cut(s) 722
PspGI CCWGG 2 cut(s) 454, 571
PspN4I GGNNCC 3 cut(s) 655, 896, 1003
PstI CTGCAG 1 cut(s) 1063
PsuI RGATCY 1 cut(s) 1140
RsaI GTAC 2 cut(s) 414, 808
RsaNI GTAC 2 cut(s) 413, 807
RseI CAYNNNNRTG 2 cut(s) 299, 900
SaqAI TTAA 4 cut(s) 126, 183, 191, 417
SatI GCNGC 1 cut(s) 496
Sau3AI GATC 5 cut(s) 428, 519, 819, 1117, 1140
ScaI AGTACT 1 cut(s) 414
SchI GAGTC 1 cut(s) 1238
ScrFI CCNGG 5 cut(s) 456, 573, 773, 1093, 1138
SfaNI GCATC 2 cut(s) 694, 893
SfcI CTRYAG 3 cut(s) 792, 1059, 1100
Sfr274I CTCGAG 1 cut(s) 204
SlaI CTCGAG 1 cut(s) 204
SmiMI CAYNNNNRTG 2 cut(s) 299, 900
SmlI CTYRAG 3 cut(s) 75, 190, 204
SmoI CTYRAG 3 cut(s) 75, 190, 204
SphI GCATGC 1 cut(s) 942
Sse9I AATT 5 cut(s) 269, 319, 364, 690, 731
SseBI AGGCCT 1 cut(s) 196
SsiI CCGC 3 cut(s) 672, 1036, 1189
SspMI CTAG 3 cut(s) 156, 198, 480
StuI AGGCCT 1 cut(s) 196
StyD4I CCNGG 5 cut(s) 454, 571, 771, 1091, 1136
TaaI ACNGT 4 cut(s) 628, 686, 806, 1104
TaiI ACGT 1 cut(s) 724
TaqI TCGA 4 cut(s) 205, 537, 702, 1080
TasI AATT 5 cut(s) 269, 319, 364, 690, 731
TatI WGTACW 2 cut(s) 412, 806
TfiI GAWTC 4 cut(s) 20, 109, 827, 971
Tru1I TTAA 4 cut(s) 126, 183, 191, 417
Tru9I TTAA 4 cut(s) 126, 183, 191, 417
TscAI CASTG 3 cut(s) 119, 386, 733
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 1 cut(s) 495
Tsp45I GTSAC 1 cut(s) 286
TspGWI ACGGA 1 cut(s) 617
TspRI CASTG 3 cut(s) 119, 386, 733
Vha464I CTTAAG 1 cut(s) 190
XapI RAATTY 3 cut(s) 319, 690, 731
XceI RCATGY 1 cut(s) 942
XhoI CTCGAG 1 cut(s) 204
XspI CTAG 3 cut(s) 156, 198, 480
ZrmI AGTACT 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.