Rh7AG483500

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001698
Physical Location & Seq
Reverse (-)
68190867 .. 68215688
24822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG483500.1

Sequence Viewer

Length: 1152 bp
ATGGCGTATTTCCAGAACGATGACCTCGAGTACGTCGTCAACGACTACTACGGCGACGTCGTTGAGTTTGAGGAAGACAACGACGCTTTTGCTGATAACCAGCCCCGGCGAAGCCTTGAGGAGTGCTTGCAAGTGGAGAAGGGGAAGAGGTTCTATGACTTTCACTACAACACAAGGCTTGTGAAGTCGACAATTGTGCATTTTCAGCTAAGGAATTTGTTATGGGCCACATCAAAGCATGATGTATACCTGATGCAGAACTTCTCAGTGATGCATTGGAACTCTTTGCACAGGAGAGGCAGCGAAGTACTCAATGTGGCCAAACCAATTGTACCTACCGAGAAACAACCTGGGTTGTTGGCACGACCACTCTCTAGAGTGCAGATAAGCACTATGGCTGTCAAGGACAATTTGTTGGTTGCTGGGGGCTTCCAAGGTGAACTAATCTGCAAGCACATGAAACAACCTGGAGTTGCTTTCTGCTCAAAACTAACAACAGATGAGAATGCCATCACTAATGCAGTGGATATTTTCCAAAACCCAACTGGCTCAATGAGGGTAATGACTGCAAATAATGATGCTCAAGTTAGAGTTTTTGACATTGAAAAGTTCGCTTGCCTCAACCGCTTCTCCTTTGAGTGGTCTGTAAATAACACCTCTGTTAGTCCCGATGGTAAGCTGCTTGCAGTACTTGGTGATAGTGCTGACTGTTTGATAGCTGATGCTCAGTCTGGAAAAGCTGTCGGGGGCCTCAAAGGGCACTTAGACTATTGCTTTTCTTCAGCTTGGCACCCGGATGGACGAATCTTGGCTACTGGAAACCAAGACACTACCTGTAGGCTATGGGACGTAAGGAACCTGTCAGAGTCCCTAGCCGTGTTGAAGGGAAGAATGGGAGCAATAAGAGCTATAAAATTCAACTCTGATGGTCGTCTTATGGCCATGGCTGAGCCAGCAGACTTTGTCTACATCATTGATACCCAGTCCGGATATGCAAAGGGACAAGAGATTGATCTGTTTGGTGAGATTGCCGGAATATCCTTCAGCCCCGACTCTGAAGCTTTGTTTGTTGGGGTTGCTGATCGCACATATGGTAGTGTATTGGAGTTCAATAAGAGGCACTATAATCCCTATCAAGAAATAGTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

383

Amino Acids

42.95

Weight (kDa)

5.43

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 132 - 328 1.8e-18 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 177 - 355 2.3e-26 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 183 - 286 8.8e-12 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 184 - 289 7.4e-07 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 186 - 363 3.9e-21 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 186 - 329 7.2e-16 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 186 - 291 8.4e-07 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 192 - 363 9.5e-13 WDR3 first beta-propeller domain
Beta-prop_IFT122_1st PF23381 212 - 335 5.5e-06 IFT122 first beta-propeller
WDR55 PF24796 215 - 359 3.4e-07 WDR55
WD40_CDC20-Fz PF24807 219 - 354 9.8e-10 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 225 - 315 7e-07 G protein beta WD-40 repeat protein
WD40 PF00400 246 - 283 2e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 60
AccB1I GGYRCC 1 cut(s) 789
AccI GTMKAC 3 cut(s) 188, 246, 966
AccIII TCCGGA 1 cut(s) 986
AciI CCGC 1 cut(s) 625
AcoI YGGCCR 2 cut(s) 318, 939
AcsI RAATTY 2 cut(s) 214, 914
AcuI CTGAAG 3 cut(s) 765, 1027, 1077
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 4 cut(s) 32, 309, 333, 690
AfiI CCNNNNNNNGG 1 cut(s) 639
AgsI TTSAA 4 cut(s) 605, 883, 919, 1111
AjnI CCWGG 2 cut(s) 349, 466
AluBI AGCT 7 cut(s) 208, 679, 719, 740, 785, 908, 1061
AluI AGCT 7 cut(s) 208, 679, 719, 740, 785, 908, 1061
Ama87I CYCGRG 1 cut(s) 26
Aor13HI TCCGGA 1 cut(s) 986
AoxI GGCC 4 cut(s) 225, 318, 748, 939
ApeKI GCWGC 2 cut(s) 300, 679
ApoI RAATTY 2 cut(s) 214, 914
Asp700I GAANNNNTTC 1 cut(s) 149
AspS9I GGNCC 2 cut(s) 225, 748
AsuC2I CCSGG 2 cut(s) 106, 794
AsuHPI GGTGA 3 cut(s) 449, 707, 1034
AvaI CYCGRG 1 cut(s) 26
BaeGI GKGCMC 1 cut(s) 762
BalI TGGCCA 2 cut(s) 320, 941
BanI GGYRCC 1 cut(s) 789
BbsI GAAGAC 2 cut(s) 81, 1138
BbvI GCAGC 2 cut(s) 312, 666
BccI CCATC 4 cut(s) 518, 665, 791, 920
BceAI ACGGC 2 cut(s) 67, 860
BcgI CGANNNNNNTGC 4 cut(s) 71, 105, 178, 212
BciT130I CCWGG 2 cut(s) 351, 468
BcnI CCSGG 2 cut(s) 106, 794
BfaI CTAG 3 cut(s) 375, 872, 1150
BfmI CTRYAG 1 cut(s) 835
BisI GCNGC 2 cut(s) 301, 680
BlpI GCTNAGC 1 cut(s) 948
BlsI GCNGC 2 cut(s) 302, 681
BmcAI AGTACT 2 cut(s) 309, 690
Bme1390I CCNGG 4 cut(s) 106, 351, 468, 794
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 2 cut(s) 225, 748
BmiI GGNNCC 3 cut(s) 749, 791, 857
BmrFI CCNGG 4 cut(s) 106, 351, 468, 794
BmrI ACTGGG 1 cut(s) 976
BmsI GCATC 4 cut(s) 243, 261, 568, 712
BmuI ACTGGG 1 cut(s) 976
BpiI GAAGAC 2 cut(s) 81, 1138
BpmI CTGGAG 1 cut(s) 489
Bpu10I CCTNAGC 1 cut(s) 209
Bpu1102I GCTNAGC 1 cut(s) 948
BpuEI CTTGAG 2 cut(s) 137, 567
BpuMI CCSGG 2 cut(s) 106, 794
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 4 cut(s) 104, 350, 433, 942
BsaWI WCCGGW 1 cut(s) 986
BsaXI ACNNNNNCTCC 2 cut(s) 614, 644
Bsc4I CCNNNNNNNGG 1 cut(s) 639
Bse1I ACTGG 3 cut(s) 550, 820, 982
BseAI TCCGGA 1 cut(s) 986
BseBI CCWGG 2 cut(s) 351, 468
BseDI CCNNGG 4 cut(s) 104, 350, 433, 942
BseGI GGATG 1 cut(s) 802
BseLI CCNNNNNNNGG 1 cut(s) 639
BseMII CTCAG 3 cut(s) 279, 740, 939
BseNI ACTGG 3 cut(s) 550, 820, 982
BseRI GAGGAG 1 cut(s) 134
BseSI GKGCMC 1 cut(s) 762
BseXI GCAGC 2 cut(s) 312, 666
BseYI CCCAGC 1 cut(s) 422
BsgI GTGCAG 1 cut(s) 401
BshFI GGCC 4 cut(s) 227, 320, 750, 941
BshNI GGYRCC 1 cut(s) 789
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 4 cut(s) 106, 794, 987, 1032
BslFI GGGAC 4 cut(s) 651, 853, 860, 1014
BslI CCNNNNNNNGG 1 cut(s) 639
BsmFI GGGAC 4 cut(s) 651, 853, 860, 1014
BsmI GAATGC 1 cut(s) 511
BsnI GGCC 4 cut(s) 227, 320, 750, 941
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 762
Bsp13I TCCGGA 1 cut(s) 986
Bsp143I GATC 2 cut(s) 1012, 1081
Bsp1720I GCTNAGC 1 cut(s) 948
Bsp19I CCATGG 1 cut(s) 942
BspACI CCGC 1 cut(s) 625
BspANI GGCC 4 cut(s) 227, 320, 750, 941
BspCNI CTCAG 3 cut(s) 278, 739, 940
BspEI TCCGGA 1 cut(s) 986
BspLI GGNNCC 3 cut(s) 749, 791, 857
BspT107I GGYRCC 1 cut(s) 789
BsrI ACTGG 3 cut(s) 550, 820, 982
BssECI CCNNGG 4 cut(s) 104, 350, 433, 942
BssMI GATC 2 cut(s) 1012, 1081
BssNAI GTATAC 1 cut(s) 247
BssNI GRCGYC 1 cut(s) 57
BssT1I CCWWGG 2 cut(s) 433, 942
Bst1107I GTATAC 1 cut(s) 247
Bst2UI CCWGG 2 cut(s) 351, 468
Bst4CI ACNGT 1 cut(s) 710
Bst6I CTCTTC 1 cut(s) 140
BstACI GRCGYC 1 cut(s) 57
BstC8I GCNNGC 5 cut(s) 128, 452, 616, 684, 954
BstDEI CTNAG 5 cut(s) 209, 265, 726, 763, 948
BstDSI CCRYGG 1 cut(s) 942
BstF5I GGATG 1 cut(s) 802
BstKTI GATC 2 cut(s) 1015, 1084
BstMBI GATC 2 cut(s) 1012, 1081
BstMWI GCNNNNNNNGC 4 cut(s) 205, 624, 905, 953
BstNI CCWGG 2 cut(s) 351, 468
BstSCI CCNGG 4 cut(s) 104, 349, 466, 792
BstSFI CTRYAG 1 cut(s) 835
BstSLI GKGCMC 1 cut(s) 762
BstV1I GCAGC 2 cut(s) 312, 666
BstV2I GAAGAC 2 cut(s) 81, 1138
BstZ17I GTATAC 1 cut(s) 247
BsuRI GGCC 4 cut(s) 227, 320, 750, 941
BtgI CCRYGG 1 cut(s) 942
BtsCI GGATG 1 cut(s) 802
BtsI GCAGTG 1 cut(s) 528
BtsIMutI CAGTG 2 cut(s) 273, 528
Cac8I GCNNGC 5 cut(s) 128, 452, 616, 684, 954
Cfr13I GGNCC 2 cut(s) 225, 748
CseI GACGC 1 cut(s) 92
Csp6I GTAC 4 cut(s) 31, 308, 332, 689
CviAII CATG 3 cut(s) 239, 457, 943
CviQI GTAC 4 cut(s) 31, 308, 332, 689
DdeI CTNAG 5 cut(s) 209, 265, 726, 763, 948
DpnI GATC 2 cut(s) 1014, 1083
DpnII GATC 2 cut(s) 1012, 1081
EaeI YGGCCR 2 cut(s) 318, 939
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Eco130I CCWWGG 2 cut(s) 433, 942
Eco57I CTGAAG 3 cut(s) 765, 1027, 1077
Eco88I CYCGRG 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 748
EcoRII CCWGG 2 cut(s) 349, 466
EcoT14I CCWWGG 2 cut(s) 433, 942
EcoT22I ATGCAT 1 cut(s) 276
ErhI CCWWGG 2 cut(s) 433, 942
FaeI CATG 3 cut(s) 242, 460, 946
FaqI GGGAC 4 cut(s) 651, 853, 860, 1014
FatI CATG 3 cut(s) 238, 456, 942
FauNDI CATATG 1 cut(s) 1090
FblI GTMKAC 3 cut(s) 188, 246, 966
Fnu4HI GCNGC 2 cut(s) 301, 680
FokI GGATG 1 cut(s) 809
Fsp4HI GCNGC 2 cut(s) 301, 680
FspBI CTAG 3 cut(s) 375, 872, 1150
GluI GCNGC 2 cut(s) 301, 680
GsaI CCCAGC 1 cut(s) 426
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 4 cut(s) 227, 320, 750, 941
HapII CCGG 4 cut(s) 106, 794, 987, 1032
HgaI GACGC 1 cut(s) 92
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 3 cut(s) 242, 460, 946
HincII GTYRAC 2 cut(s) 40, 189
HindII GTYRAC 2 cut(s) 40, 189
HindIII AAGCTT 1 cut(s) 1059
HinfI GANTC 3 cut(s) 804, 866, 1052
HpaII CCGG 4 cut(s) 106, 794, 987, 1032
HphI GGTGA 3 cut(s) 449, 707, 1034
Hpy166II GTNNAC 5 cut(s) 40, 189, 247, 440, 967
Hpy188I TCNGA 3 cut(s) 865, 925, 1057
Hpy188III TCNNGA 6 cut(s) 13, 375, 668, 732, 987, 1136
Hpy8I GTNNAC 5 cut(s) 40, 189, 247, 440, 967
Hpy99I CGWCG 4 cut(s) 38, 59, 62, 86
HpyAV CCTTC 3 cut(s) 133, 877, 1051
HpyCH4III ACNGT 1 cut(s) 710
HpyCH4IV ACGT 3 cut(s) 33, 57, 849
HpyF10VI GCNNNNNNNGC 4 cut(s) 205, 624, 905, 953
HpyF3I CTNAG 5 cut(s) 209, 265, 726, 763, 948
HpySE526I ACGT 3 cut(s) 33, 57, 849
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 3 cut(s) 242, 460, 946
Kpn2I TCCGGA 1 cut(s) 986
Kzo9I GATC 2 cut(s) 1012, 1081
LmnI GCTCC 1 cut(s) 896
Lsp1109I GCAGC 2 cut(s) 312, 666
LweI GCATC 4 cut(s) 243, 261, 568, 712
MaeI CTAG 3 cut(s) 375, 872, 1150
MaeII ACGT 3 cut(s) 33, 57, 849
MalI GATC 2 cut(s) 1014, 1083
MboI GATC 2 cut(s) 1012, 1081
MboII GAAGA 5 cut(s) 86, 157, 771, 900, 1138
MfeI CAATTG 2 cut(s) 192, 327
MhlI GDGCHC 1 cut(s) 762
MlsI TGGCCA 2 cut(s) 320, 941
MluCI AATT 5 cut(s) 192, 214, 327, 409, 914
MluNI TGGCCA 2 cut(s) 320, 941
MlyI GAGTC 2 cut(s) 875, 1046
Mox20I TGGCCA 2 cut(s) 320, 941
Mph1103I ATGCAT 1 cut(s) 276
MroI TCCGGA 1 cut(s) 986
MroXI GAANNNNTTC 1 cut(s) 149
MscI TGGCCA 2 cut(s) 320, 941
MslI CAYNNNNRTG 1 cut(s) 795
Msp20I TGGCCA 2 cut(s) 320, 941
MspI CCGG 4 cut(s) 106, 794, 987, 1032
MspR9I CCNGG 4 cut(s) 106, 351, 468, 794
MunI CAATTG 2 cut(s) 192, 327
Mva1269I GAATGC 1 cut(s) 511
MvaI CCWGG 2 cut(s) 351, 468
MwoI GCNNNNNNNGC 4 cut(s) 205, 624, 905, 953
NciI CCSGG 2 cut(s) 106, 794
NcoI CCATGG 1 cut(s) 942
NdeI CATATG 1 cut(s) 1090
NdeII GATC 2 cut(s) 1012, 1081
NlaIII CATG 3 cut(s) 242, 460, 946
NlaIV GGNNCC 3 cut(s) 749, 791, 857
NsiI ATGCAT 1 cut(s) 276
PaeR7I CTCGAG 1 cut(s) 26
PcsI WCGNNNNNNNCGW 4 cut(s) 24, 33, 39, 57
PctI GAATGC 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 1 cut(s) 804
PflFI GACNNNGTC 1 cut(s) 962
PkrI GCNGC 2 cut(s) 302, 681
PleI GAGTC 2 cut(s) 874, 1046
PpsI GAGTC 2 cut(s) 874, 1046
Psp6I CCWGG 2 cut(s) 349, 466
PspFI CCCAGC 1 cut(s) 422
PspGI CCWGG 2 cut(s) 349, 466
PspN4I GGNNCC 3 cut(s) 749, 791, 857
PspPI GGNCC 2 cut(s) 225, 748
PspXI VCTCGAGB 1 cut(s) 26
PsyI GACNNNGTC 1 cut(s) 962
RsaI GTAC 4 cut(s) 32, 309, 333, 690
RsaNI GTAC 4 cut(s) 31, 308, 332, 689
RseI CAYNNNNRTG 1 cut(s) 795
SalI GTCGAC 1 cut(s) 187
SatI GCNGC 2 cut(s) 301, 680
Sau3AI GATC 2 cut(s) 1012, 1081
Sau96I GGNCC 2 cut(s) 225, 748
ScaI AGTACT 2 cut(s) 309, 690
SchI GAGTC 2 cut(s) 875, 1046
ScrFI CCNGG 4 cut(s) 106, 351, 468, 794
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 4 cut(s) 243, 261, 568, 712
SfcI CTRYAG 1 cut(s) 835
Sfr274I CTCGAG 1 cut(s) 26
SlaI CTCGAG 1 cut(s) 26
SmiMI CAYNNNNRTG 1 cut(s) 795
SmlI CTYRAG 3 cut(s) 26, 116, 582
SmoI CTYRAG 3 cut(s) 26, 116, 582
Sse9I AATT 5 cut(s) 192, 214, 327, 409, 914
SsiI CCGC 1 cut(s) 625
SspMI CTAG 3 cut(s) 375, 872, 1150
StyD4I CCNGG 4 cut(s) 104, 349, 466, 792
StyI CCWWGG 2 cut(s) 433, 942
TaaI ACNGT 1 cut(s) 710
TaiI ACGT 3 cut(s) 36, 60, 852
TaqI TCGA 2 cut(s) 27, 188
TasI AATT 5 cut(s) 192, 214, 327, 409, 914
TatI WGTACW 2 cut(s) 307, 688
TfiI GAWTC 1 cut(s) 804
TscAI CASTG 2 cut(s) 273, 528
TseI GCWGC 2 cut(s) 300, 679
TspDTI ATGAA 1 cut(s) 473
TspRI CASTG 2 cut(s) 273, 528
Tth111I GACNNNGTC 1 cut(s) 962
XapI RAATTY 2 cut(s) 214, 914
XbaI TCTAGA 1 cut(s) 374
XcmI CCANNNNNNNNNTGG 1 cut(s) 542
XhoI CTCGAG 1 cut(s) 26
XmiI GTMKAC 3 cut(s) 188, 246, 966
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 3 cut(s) 375, 872, 1150
ZraI GACGTC 1 cut(s) 58
ZrmI AGTACT 2 cut(s) 309, 690
Zsp2I ATGCAT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.