Rw7G040560

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001698
Physical Location & Seq
Reverse (-)
63174638 .. 63195170
20533 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G040560.1

Sequence Viewer

Length: 1374 bp
ATGGCGTATTTCCAGAACGATGACCTCGAGTACGTCGTCAACGACTACTACGGCGACGTCGTTGAGTTTGAGGAAGACAACGACGCTTTTGCTGATAACCAGCCCCGGCGAAGCCTTGAGGTCGTAGACTCCGACTCCGACTCCGACGACGACTTTGAAGACGACCTTGATTCGAGCAAGGTGAAGAGTGATACATCGGCTTTGGAAGCTAGAAATGGAAAAGACATACAGGGAATACCATGGGAGAGGCTTAATTTCACCAGAGATAAGTACCGTGAGGCGCGATTGAAGCAGTATAAGAACTATGAGAGCCTCGGTCGTCCTCGTCAAGACCTCGAGAAGGAGTGCTTGCAAGTGGAGAAGGGGAAGAGGTTCTATGACTTTCACTACAACACAAGGCTTGTGAAGTCGACAATTGTGCATTTTCAGCTAAGGAATTTGTTATGGGCCACATCAAAGCATGATGTATACCTGATGCAGAACTTCTCAGTGATGCATTGGAACTCTTTGCACAGGAGAGGCAGCGAAGTACTCAATGTGGCCAAACCAATTGTACCTACCGAGAAACAACCTGGGTTGTTGGCACGACCACTCTCTAGAGTGCAGATAAGCACTATGGCTGTCAAGGACAATTTGTTGGTTGCTGGGGGCTTCCAAGGTGAACTAATCTGCAAGCACATGAAACAACCTGGAGTTGCTTTCTGCTCAAAACTAACAACAGATGAGAATGCCATCACTAATGCAGTGGATATTTTCCAAAACCCAACTGGCTCAATGAGGGTAATGACTGCAAATAATGATGCTCAAGTTAGAGTTTTTGACATTGAAAAGTTCGCTTGCCTCAACCGCTTCTCCTTTGAGTGGTCTGTAAATAACACTTCTGTTAGTCCCGATGGTAAGCTGCTCGCAGTACTTGGTGATAGTGCAGACTGTTTGATAGCTGATGCTCAGTCTGGAAAAGCTGTCGGGGGCCTCAAAGGGCACTTAGACTATTGCTTTTCCTCAGCTTGGCACCCGGATGGGCGAATCTTAGCTACTGGGAACCAAGACACTACTTGCAGGTTATGGGACATAAGGAACCTGTCAGAGTCCCTAGTCGTGTTGAAGGGAAGAATGGGAGCAGTAAGGGCTATAAAATTCACCTCTGATGGTCGTTTTATGGCCATGGCTGAGCCAGCAGACTTTGTTCACATCATTGATACCCAGTCCGGATATGCAAAGGAACAAGAGATTGATCTGTTTGGTGAGATTGCCGGAATATCCTTCAGCCCCGACTCTGAAGCTTTGTTTATTGGGGTTGCTGATCGCACATATGGTAGTGTATTGGAGTTCAATAAGAGGCACTATAATACCTATGAAGAAATAGTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

457

Amino Acids

51.7

Weight (kDa)

5.12

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 206 - 404 1.5e-18 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 251 - 429 2e-26 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 258 - 390 1.1e-12 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 258 - 363 6e-07 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 260 - 438 1.2e-20 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 260 - 404 1.7e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 260 - 364 1.1e-06 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 266 - 437 9.2e-14 WDR3 first beta-propeller domain
WDR55 PF24796 286 - 432 5.7e-07 WDR55
WD40 PF00400 320 - 357 2.6e-07 WD domain, G-beta repeat
WD40_Gbeta PF25391 326 - 437 5.6e-06 G protein beta WD-40 repeat protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 60
Acc36I ACCTGC 1 cut(s) 1050
AccB1I GGYRCC 1 cut(s) 1011
AccI GTMKAC 3 cut(s) 126, 410, 468
AccII CGCG 1 cut(s) 283
AccIII TCCGGA 1 cut(s) 1208
AciI CCGC 1 cut(s) 847
AcoI YGGCCR 2 cut(s) 540, 1161
AcsI RAATTY 2 cut(s) 436, 1136
AcuI CTGAAG 2 cut(s) 1249, 1299
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 5 cut(s) 32, 272, 531, 555, 912
AfiI CCNNNNNNNGG 3 cut(s) 340, 861, 1008
AgsI TTSAA 5 cut(s) 158, 289, 827, 1105, 1333
AjnI CCWGG 2 cut(s) 571, 688
AluBI AGCT 8 cut(s) 209, 430, 901, 941, 962, 1007, 1034, 1283
AluI AGCT 8 cut(s) 209, 430, 901, 941, 962, 1007, 1034, 1283
Ama87I CYCGRG 2 cut(s) 26, 335
Aor13HI TCCGGA 1 cut(s) 1208
AoxI GGCC 4 cut(s) 447, 540, 970, 1161
ApeKI GCWGC 2 cut(s) 522, 901
ApoI RAATTY 2 cut(s) 436, 1136
ArsI GACNNNNNNTTYG 2 cut(s) 137, 169
Asp700I GAANNNNTTC 1 cut(s) 371
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 2 cut(s) 447, 970
AsuC2I CCSGG 2 cut(s) 106, 1016
AsuHPI GGTGA 6 cut(s) 193, 250, 671, 929, 1132, 1256
AvaI CYCGRG 2 cut(s) 26, 335
BaeGI GKGCMC 1 cut(s) 984
BalI TGGCCA 2 cut(s) 542, 1163
BanI GGYRCC 1 cut(s) 1011
BbsI GAAGAC 3 cut(s) 81, 165, 1360
BbvCI CCTCAGC 1 cut(s) 1003
BbvI GCAGC 2 cut(s) 534, 888
BccI CCATC 4 cut(s) 740, 887, 1013, 1142
BceAI ACGGC 1 cut(s) 67
BcgI CGANNNNNNTGC 4 cut(s) 71, 105, 400, 434
BciT130I CCWGG 2 cut(s) 573, 690
BcnI CCSGG 2 cut(s) 106, 1016
BfaI CTAG 4 cut(s) 210, 597, 1094, 1372
BfuAI ACCTGC 1 cut(s) 1050
BisI GCNGC 2 cut(s) 523, 902
BlpI GCTNAGC 1 cut(s) 1170
BlsI GCNGC 2 cut(s) 524, 903
BmcAI AGTACT 2 cut(s) 531, 912
Bme1390I CCNGG 4 cut(s) 106, 573, 690, 1016
BmeT110I CYCGRG 2 cut(s) 26, 335
BmgT120I GGNCC 2 cut(s) 447, 970
BmiI GGNNCC 4 cut(s) 971, 1013, 1043, 1079
BmrFI CCNGG 4 cut(s) 106, 573, 690, 1016
BmrI ACTGGG 2 cut(s) 1047, 1198
BmsI GCATC 4 cut(s) 465, 483, 790, 934
BmuI ACTGGG 2 cut(s) 1047, 1198
BpiI GAAGAC 3 cut(s) 81, 165, 1360
BpmI CTGGAG 1 cut(s) 711
Bpu10I CCTNAGC 2 cut(s) 431, 1003
Bpu1102I GCTNAGC 1 cut(s) 1170
BpuEI CTTGAG 2 cut(s) 137, 789
BpuMI CCSGG 2 cut(s) 106, 1016
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 6 cut(s) 104, 239, 313, 572, 655, 1164
BsaWI WCCGGW 1 cut(s) 1208
BsaXI ACNNNNNCTCC 6 cut(s) 119, 125, 149, 155, 836, 866
Bsc4I CCNNNNNNNGG 3 cut(s) 340, 861, 1008
Bse1I ACTGG 3 cut(s) 772, 1042, 1204
BseAI TCCGGA 1 cut(s) 1208
BseBI CCWGG 2 cut(s) 573, 690
BseDI CCNNGG 6 cut(s) 104, 239, 313, 572, 655, 1164
BseGI GGATG 1 cut(s) 1024
BseLI CCNNNNNNNGG 3 cut(s) 340, 861, 1008
BseMII CTCAG 4 cut(s) 501, 962, 1017, 1161
BseNI ACTGG 3 cut(s) 772, 1042, 1204
BseSI GKGCMC 1 cut(s) 984
BseXI GCAGC 2 cut(s) 534, 888
BseYI CCCAGC 1 cut(s) 644
BsgI GTGCAG 2 cut(s) 623, 945
Bsh1236I CGCG 1 cut(s) 283
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 4 cut(s) 449, 542, 972, 1163
BshNI GGYRCC 1 cut(s) 1011
BsiEI CGRYCG 1 cut(s) 319
BsiHKCI CYCGRG 2 cut(s) 26, 335
BsiSI CCGG 4 cut(s) 106, 1016, 1209, 1254
BslFI GGGAC 3 cut(s) 873, 1075, 1082
BslI CCNNNNNNNGG 3 cut(s) 340, 861, 1008
BsmFI GGGAC 3 cut(s) 873, 1075, 1082
BsmI GAATGC 1 cut(s) 733
BsnI GGCC 4 cut(s) 449, 542, 972, 1163
BsoBI CYCGRG 2 cut(s) 26, 335
Bsp1286I GDGCHC 1 cut(s) 984
Bsp13I TCCGGA 1 cut(s) 1208
Bsp143I GATC 2 cut(s) 1234, 1303
Bsp1720I GCTNAGC 1 cut(s) 1170
Bsp19I CCATGG 2 cut(s) 239, 1164
BspACI CCGC 1 cut(s) 847
BspANI GGCC 4 cut(s) 449, 542, 972, 1163
BspCNI CTCAG 4 cut(s) 500, 961, 1016, 1162
BspEI TCCGGA 1 cut(s) 1208
BspFNI CGCG 1 cut(s) 283
BspLI GGNNCC 4 cut(s) 971, 1013, 1043, 1079
BspMI ACCTGC 1 cut(s) 1050
BspT107I GGYRCC 1 cut(s) 1011
BsrI ACTGG 3 cut(s) 772, 1042, 1204
BssECI CCNNGG 6 cut(s) 104, 239, 313, 572, 655, 1164
BssMI GATC 2 cut(s) 1234, 1303
BssNAI GTATAC 1 cut(s) 469
BssNI GRCGYC 1 cut(s) 57
BssT1I CCWWGG 3 cut(s) 239, 655, 1164
Bst1107I GTATAC 1 cut(s) 469
Bst2UI CCWGG 2 cut(s) 573, 690
Bst4CI ACNGT 2 cut(s) 275, 932
Bst6I CTCTTC 2 cut(s) 179, 362
BstACI GRCGYC 1 cut(s) 57
BstC8I GCNNGC 5 cut(s) 350, 674, 838, 906, 1176
BstDEI CTNAG 7 cut(s) 431, 487, 948, 985, 1003, 1030, 1170
BstDSI CCRYGG 2 cut(s) 239, 1164
BstENI CCTNNNNNAGG 1 cut(s) 338
BstF5I GGATG 1 cut(s) 1024
BstFNI CGCG 1 cut(s) 283
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 2 cut(s) 1237, 1306
BstMBI GATC 2 cut(s) 1234, 1303
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 6 cut(s) 206, 289, 427, 846, 1127, 1175
BstNI CCWGG 2 cut(s) 573, 690
BstSCI CCNGG 4 cut(s) 104, 571, 688, 1014
BstSLI GKGCMC 1 cut(s) 984
BstUI CGCG 1 cut(s) 283
BstV1I GCAGC 2 cut(s) 534, 888
BstV2I GAAGAC 3 cut(s) 81, 165, 1360
BstZ17I GTATAC 1 cut(s) 469
BsuRI GGCC 4 cut(s) 449, 542, 972, 1163
BtgI CCRYGG 2 cut(s) 239, 1164
BtsCI GGATG 1 cut(s) 1024
BtsI GCAGTG 1 cut(s) 750
BtsIMutI CAGTG 2 cut(s) 495, 750
BveI ACCTGC 1 cut(s) 1050
Cac8I GCNNGC 5 cut(s) 350, 674, 838, 906, 1176
CfoI GCGC 1 cut(s) 283
Cfr13I GGNCC 2 cut(s) 447, 970
CseI GACGC 1 cut(s) 92
Csp6I GTAC 5 cut(s) 31, 271, 530, 554, 911
CviAII CATG 4 cut(s) 240, 461, 679, 1165
CviQI GTAC 5 cut(s) 31, 271, 530, 554, 911
DdeI CTNAG 7 cut(s) 431, 487, 948, 985, 1003, 1030, 1170
DpnI GATC 2 cut(s) 1236, 1305
DpnII GATC 2 cut(s) 1234, 1303
EaeI YGGCCR 2 cut(s) 540, 1161
Eam1104I CTCTTC 2 cut(s) 179, 362
EarI CTCTTC 2 cut(s) 179, 362
Eco130I CCWWGG 3 cut(s) 239, 655, 1164
Eco57I CTGAAG 2 cut(s) 1249, 1299
Eco88I CYCGRG 2 cut(s) 26, 335
EcoNI CCTNNNNNAGG 1 cut(s) 338
EcoO109I RGGNCCY 1 cut(s) 970
EcoRII CCWGG 2 cut(s) 571, 688
EcoT14I CCWWGG 3 cut(s) 239, 655, 1164
EcoT22I ATGCAT 1 cut(s) 498
ErhI CCWWGG 3 cut(s) 239, 655, 1164
FaeI CATG 4 cut(s) 243, 464, 682, 1168
FalI AAGNNNNNCTT 4 cut(s) 150, 182, 332, 364
FaqI GGGAC 3 cut(s) 873, 1075, 1082
FatI CATG 4 cut(s) 239, 460, 678, 1164
FauNDI CATATG 1 cut(s) 1312
FblI GTMKAC 3 cut(s) 126, 410, 468
Fnu4HI GCNGC 2 cut(s) 523, 902
FokI GGATG 1 cut(s) 1031
Fsp4HI GCNGC 2 cut(s) 523, 902
FspBI CTAG 4 cut(s) 210, 597, 1094, 1372
GlaI GCGC 1 cut(s) 282
GluI GCNGC 2 cut(s) 523, 902
GsaI CCCAGC 1 cut(s) 648
GsuI CTGGAG 1 cut(s) 711
HaeIII GGCC 4 cut(s) 449, 542, 972, 1163
HapII CCGG 4 cut(s) 106, 1016, 1209, 1254
HgaI GACGC 1 cut(s) 92
HhaI GCGC 1 cut(s) 283
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 4 cut(s) 243, 464, 682, 1168
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HincII GTYRAC 2 cut(s) 40, 411
HindII GTYRAC 2 cut(s) 40, 411
HindIII AAGCTT 1 cut(s) 1281
HinfI GANTC 7 cut(s) 128, 134, 140, 170, 1026, 1088, 1274
HpaII CCGG 4 cut(s) 106, 1016, 1209, 1254
HphI GGTGA 6 cut(s) 193, 250, 671, 929, 1132, 1256
Hpy166II GTNNAC 6 cut(s) 40, 127, 411, 469, 662, 1189
Hpy188I TCNGA 6 cut(s) 133, 139, 145, 1087, 1147, 1279
Hpy188III TCNNGA 7 cut(s) 13, 329, 337, 597, 890, 954, 1209
Hpy8I GTNNAC 6 cut(s) 40, 127, 411, 469, 662, 1189
Hpy99I CGWCG 6 cut(s) 38, 59, 62, 86, 149, 152
HpyAV CCTTC 4 cut(s) 334, 355, 1099, 1273
HpyCH4III ACNGT 2 cut(s) 275, 932
HpyCH4IV ACGT 2 cut(s) 33, 57
HpyF10VI GCNNNNNNNGC 6 cut(s) 206, 289, 427, 846, 1127, 1175
HpyF3I CTNAG 7 cut(s) 431, 487, 948, 985, 1003, 1030, 1170
HpySE526I ACGT 2 cut(s) 33, 57
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 4 cut(s) 243, 464, 682, 1168
HspAI GCGC 1 cut(s) 281
Kpn2I TCCGGA 1 cut(s) 1208
Kzo9I GATC 2 cut(s) 1234, 1303
LmnI GCTCC 1 cut(s) 1118
Lsp1109I GCAGC 2 cut(s) 534, 888
LweI GCATC 4 cut(s) 465, 483, 790, 934
MaeI CTAG 4 cut(s) 210, 597, 1094, 1372
MaeII ACGT 2 cut(s) 33, 57
MalI GATC 2 cut(s) 1236, 1305
MboI GATC 2 cut(s) 1234, 1303
MboII GAAGA 7 cut(s) 86, 170, 196, 379, 1122, 1360, 1370
MfeI CAATTG 2 cut(s) 414, 549
MhlI GDGCHC 1 cut(s) 984
MlsI TGGCCA 2 cut(s) 542, 1163
MluCI AATT 6 cut(s) 253, 414, 436, 549, 631, 1136
MluNI TGGCCA 2 cut(s) 542, 1163
MlyI GAGTC 5 cut(s) 122, 128, 134, 1097, 1268
MmeI TCCRAC 3 cut(s) 156, 162, 168
Mox20I TGGCCA 2 cut(s) 542, 1163
Mph1103I ATGCAT 1 cut(s) 498
MroI TCCGGA 1 cut(s) 1208
MroXI GAANNNNTTC 1 cut(s) 371
MscI TGGCCA 2 cut(s) 542, 1163
MseI TTAA 1 cut(s) 252
MslI CAYNNNNRTG 1 cut(s) 1017
Msp20I TGGCCA 2 cut(s) 542, 1163
MspI CCGG 4 cut(s) 106, 1016, 1209, 1254
MspR9I CCNGG 4 cut(s) 106, 573, 690, 1016
MunI CAATTG 2 cut(s) 414, 549
Mva1269I GAATGC 1 cut(s) 733
MvaI CCWGG 2 cut(s) 573, 690
MvnI CGCG 1 cut(s) 283
MwoI GCNNNNNNNGC 6 cut(s) 206, 289, 427, 846, 1127, 1175
NciI CCSGG 2 cut(s) 106, 1016
NcoI CCATGG 2 cut(s) 239, 1164
NdeI CATATG 1 cut(s) 1312
NdeII GATC 2 cut(s) 1234, 1303
NlaIII CATG 4 cut(s) 243, 464, 682, 1168
NlaIV GGNNCC 4 cut(s) 971, 1013, 1043, 1079
NsiI ATGCAT 1 cut(s) 498
PaeR7I CTCGAG 2 cut(s) 26, 335
PcsI WCGNNNNNNNCGW 5 cut(s) 24, 33, 39, 57, 129
PctI GAATGC 1 cut(s) 733
PdmI GAANNNNTTC 1 cut(s) 371
PfeI GAWTC 2 cut(s) 170, 1026
PkrI GCNGC 2 cut(s) 524, 903
PleI GAGTC 5 cut(s) 122, 128, 134, 1096, 1268
PpsI GAGTC 5 cut(s) 122, 128, 134, 1096, 1268
Psp6I CCWGG 2 cut(s) 571, 688
PspFI CCCAGC 1 cut(s) 644
PspGI CCWGG 2 cut(s) 571, 688
PspN4I GGNNCC 4 cut(s) 971, 1013, 1043, 1079
PspPI GGNCC 2 cut(s) 447, 970
PspXI VCTCGAGB 1 cut(s) 26
RsaI GTAC 5 cut(s) 32, 272, 531, 555, 912
RsaNI GTAC 5 cut(s) 31, 271, 530, 554, 911
RseI CAYNNNNRTG 1 cut(s) 1017
SalI GTCGAC 1 cut(s) 409
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 2 cut(s) 523, 902
Sau3AI GATC 2 cut(s) 1234, 1303
Sau96I GGNCC 2 cut(s) 447, 970
ScaI AGTACT 2 cut(s) 531, 912
SchI GAGTC 5 cut(s) 122, 128, 134, 1097, 1268
ScrFI CCNGG 4 cut(s) 106, 573, 690, 1016
SduI GDGCHC 1 cut(s) 984
SfaNI GCATC 4 cut(s) 465, 483, 790, 934
Sfr274I CTCGAG 2 cut(s) 26, 335
SlaI CTCGAG 2 cut(s) 26, 335
SmiMI CAYNNNNRTG 1 cut(s) 1017
SmlI CTYRAG 4 cut(s) 26, 116, 335, 804
SmoI CTYRAG 4 cut(s) 26, 116, 335, 804
Sse9I AATT 6 cut(s) 253, 414, 436, 549, 631, 1136
SsiI CCGC 1 cut(s) 847
SspMI CTAG 4 cut(s) 210, 597, 1094, 1372
StyD4I CCNGG 4 cut(s) 104, 571, 688, 1014
StyI CCWWGG 3 cut(s) 239, 655, 1164
TaaI ACNGT 2 cut(s) 275, 932
TaiI ACGT 2 cut(s) 36, 60
TaqI TCGA 4 cut(s) 27, 173, 336, 410
TaqII GACCGA 1 cut(s) 305
TasI AATT 6 cut(s) 253, 414, 436, 549, 631, 1136
TatI WGTACW 2 cut(s) 529, 910
TfiI GAWTC 2 cut(s) 170, 1026
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 2 cut(s) 495, 750
TseI GCWGC 2 cut(s) 522, 901
TspDTI ATGAA 2 cut(s) 695, 1371
TspRI CASTG 2 cut(s) 495, 750
XagI CCTNNNNNAGG 1 cut(s) 338
XapI RAATTY 2 cut(s) 436, 1136
XbaI TCTAGA 1 cut(s) 596
XcmI CCANNNNNNNNNTGG 1 cut(s) 764
XhoI CTCGAG 2 cut(s) 26, 335
XmiI GTMKAC 3 cut(s) 126, 410, 468
XmnI GAANNNNTTC 1 cut(s) 371
XspI CTAG 4 cut(s) 210, 597, 1094, 1372
ZraI GACGTC 1 cut(s) 58
ZrmI AGTACT 2 cut(s) 531, 912
Zsp2I ATGCAT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.