AT1G78070

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001698
Physical Location & Seq
Forward (+)
29354944 .. 29359032
4089 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G78070.2

Sequence Viewer

Length: 1350 bp
ATGGATATTTTCGACAACTCTGATCTTGAATACCTTGTTGATGAATTTCACGCTGATTTCGATGATGATGAGCCCTTTGGAGAGGTTGATGTCACCAGTGAGTCTGATTCCGATTTCATGGACTCCGATTTTGATTTCGAGCTGAGTGAGAGTAAGACGAACAATGAAACATCAGCGTTAGAAGCTAGGAATGGAAAAGATATTCAAGGGATTCCATGGGAGAGCTTGAATTACACGAGGGATAGATACCGTGAGAATAGATTGCTGCACTATAAAAACTTTGAGAGTCTGTTTCGATCCAGAGAAGAGCTTGATAAGGAATGCTTGCAAGTAGAGAAAGGAAAGAACTTTTATGACTTTCAGTTCAATACAAGGCTTGTCAAGTCCACAATAGCGCATTTTCAGCTGAGGAACTTGGTATGGGCAACATCAAAGCACGATGTGTATTTCATGAACAACTACTCTCTCATGCACTGGTCATCTTTGTTGCAAAGGGGCAAAGAAGTACTTAATGTGGCAAAGCCCATTGTTCCTTCAATGAAGCAGCATGGATCGTTGTCACAGTCTGTATCAAGAGTCCAGATAAGCACCATGGCAGTTAAAGACGATTTGATAGTTGCGGGAGGGTTCCAAGGAGAGCTTATCTGTAAGAAAATCAACGAACCTGAGGTTGCTTTCTGCACTAAACTAACATCAGCTGATAACGACATCACCAATTCGGTTGACATCTACAACGCACCTAGTGGCTCGTTAAGGGTTATGACCGCGAACAATGACTGTACTGTCCGGCTATTTGATGCCACAAACTTTGCCTTGCTCAACAGATTTGCCTTTCACTGGTCTGTAAATAACATCTCCACCAGTCCAGATGGTAAGCTAGTGGCTGTTCTAGGAGACAGTCCTGAGTGCTTACTAGCAGATACCGGATCTGGAAAAGTGATCCACGGACTCGAAGGCCATCTCGACTACTCGTTTTCATCAGCATGGCACCCAAACGGTCAAATCTTAGCCACGGGTAACCAAGACACAACCTGTAGACTATGGGACGTAAGGAACCTGTCTCAATCACTCAAAGTCTTGAAAGGAAACATGGGAGCTATCAGAGCCTTGAGGTTCACGTCTGATGGACGGTTCTTAGCCATGGCTGAGCCAGCGGACTTTGTACACTTGTTTGACACGGAAGCTGGTTACAGTCAGTGTCAAGAGATTGATCTGTTTGGAGAAATAGCGGGAATCTCGTTTAGCCCCGACACGGAGGCACTGTTCGTGGGAGTAGCTGATCGTACGTACGGCAGCTTGTTGGAGTTTAACAGGAAGCGAAACCACTCGTATGTTGATTCCATATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

449

Amino Acids

50.67

Weight (kDa)

4.83

Isoelectric Point (pI)

31.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 82 - 298 2e-19 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 128 - 242 4.2e-09 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 137 - 311 2.6e-24 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 141 - 285 1.7e-07 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 142 - 286 6e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 142 - 287 1.1e-10 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 142 - 270 1.6e-07 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 143 - 320 1.4e-21 WDR5 beta-propeller domain
WDR55 PF24796 171 - 328 1.2e-07 WDR55
Beta-prop_WDR3_1st PF25173 172 - 319 9.8e-13 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 173 - 317 1.2e-09 CDC20/Fizzy WD40 domain
WD40 PF00400 201 - 239 7e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 782
AccB1I GGYRCC 1 cut(s) 987
AccI GTMKAC 1 cut(s) 1036
AccII CGCG 1 cut(s) 767
AciI CCGC 4 cut(s) 620, 765, 1154, 1229
AclWI GGATC 4 cut(s) 291, 559, 934, 934
AcsI RAATTY 1 cut(s) 44
AdeI CACNNNGTG 2 cut(s) 442, 743
AfaI GTAC 5 cut(s) 507, 781, 1164, 1285, 1289
AfiI CCNNNNNNNGG 2 cut(s) 837, 1252
AgsI TTSAA 6 cut(s) 29, 206, 229, 367, 537, 1081
AjiI CACGTC 1 cut(s) 1119
AloI GAACNNNNNNTCC 2 cut(s) 1247, 1279
Alw26I GTCTC 2 cut(s) 888, 1065
AlwI GGATC 4 cut(s) 291, 559, 934, 934
AoxI GGCC 1 cut(s) 955
ApeKI GCWGC 3 cut(s) 265, 544, 1293
ApoI RAATTY 1 cut(s) 44
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
AspLEI GCGC 1 cut(s) 397
AsuHPI GGTGA 2 cut(s) 85, 703
AxyI CCTNAGG 1 cut(s) 666
BanI GGYRCC 1 cut(s) 987
BanII GRGCYC 1 cut(s) 75
BauI CACGAG 1 cut(s) 235
BbvCI CCTCAGC 1 cut(s) 407
BbvI GCAGC 3 cut(s) 252, 556, 1305
BccI CCATC 3 cut(s) 863, 966, 1118
BceAI ACGGC 1 cut(s) 1306
BcoDI GTCTC 2 cut(s) 888, 1065
BfaI CTAG 5 cut(s) 186, 741, 878, 890, 914
BfmI CTRYAG 1 cut(s) 1033
BisI GCNGC 3 cut(s) 266, 545, 1294
BlpI GCTNAGC 1 cut(s) 1146
BlsI GCNGC 3 cut(s) 267, 546, 1295
BmcAI AGTACT 1 cut(s) 507
BmgBI CACGTC 1 cut(s) 1119
BmiI GGNNCC 3 cut(s) 629, 989, 1055
BmsI GCATC 1 cut(s) 787
Bpu10I CCTNAGC 1 cut(s) 407
Bpu1102I GCTNAGC 1 cut(s) 1146
BpuEI CTTGAG 1 cut(s) 1129
BsaAI YACGTR 1 cut(s) 1287
BsaJI CCNNGG 6 cut(s) 215, 591, 631, 943, 1011, 1140
BsaWI WCCGGW 1 cut(s) 923
BsaXI ACNNNNNCTCC 2 cut(s) 1247, 1277
Bsc4I CCNNNNNNNGG 2 cut(s) 837, 1252
Bse1I ACTGG 4 cut(s) 96, 479, 842, 861
Bse21I CCTNAGG 1 cut(s) 666
BseDI CCNNGG 6 cut(s) 215, 591, 631, 943, 1011, 1140
BseLI CCNNNNNNNGG 2 cut(s) 837, 1252
BseMII CTCAG 5 cut(s) 134, 398, 657, 894, 1137
BseNI ACTGG 4 cut(s) 96, 479, 842, 861
BseXI GCAGC 3 cut(s) 252, 556, 1305
BsgI GTGCAG 2 cut(s) 251, 664
Bsh1236I CGCG 1 cut(s) 767
BshFI GGCC 1 cut(s) 957
BshNI GGYRCC 1 cut(s) 987
BsiSI CCGG 2 cut(s) 787, 924
BsiWI CGTACG 2 cut(s) 1283, 1287
BslFI GGGAC 1 cut(s) 1058
BslI CCNNNNNNNGG 2 cut(s) 837, 1252
BsmAI GTCTC 2 cut(s) 888, 1065
BsmFI GGGAC 1 cut(s) 1058
BsmI GAATGC 1 cut(s) 326
BsnI GGCC 1 cut(s) 957
Bsp1286I GDGCHC 1 cut(s) 75
Bsp1407I TGTACA 1 cut(s) 1162
Bsp143I GATC 7 cut(s) 22, 296, 551, 926, 939, 1210, 1279
Bsp1720I GCTNAGC 1 cut(s) 1146
Bsp19I CCATGG 3 cut(s) 215, 591, 1140
BspACI CCGC 4 cut(s) 620, 765, 1154, 1229
BspANI GGCC 1 cut(s) 957
BspCNI CTCAG 5 cut(s) 135, 399, 658, 895, 1138
BspFNI CGCG 1 cut(s) 767
BspHI TCATGA 1 cut(s) 450
BspLI GGNNCC 3 cut(s) 629, 989, 1055
BspPI GGATC 4 cut(s) 291, 559, 934, 934
BspQI GCTCTTC 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 987
BsrGI TGTACA 1 cut(s) 1162
BsrI ACTGG 4 cut(s) 96, 479, 842, 861
BssECI CCNNGG 6 cut(s) 215, 591, 631, 943, 1011, 1140
BssMI GATC 7 cut(s) 22, 296, 551, 926, 939, 1210, 1279
BssSI CACGAG 1 cut(s) 235
BssT1I CCWWGG 4 cut(s) 215, 591, 631, 1140
Bst2BI CACGAG 1 cut(s) 235
Bst4CI ACNGT 9 cut(s) 251, 564, 779, 784, 899, 998, 1131, 1193, 1263
Bst6I CTCTTC 1 cut(s) 300
BstAUI TGTACA 1 cut(s) 1162
BstBAI YACGTR 1 cut(s) 1287
BstC8I GCNNGC 2 cut(s) 326, 1152
BstDEI CTNAG 7 cut(s) 143, 407, 666, 903, 1006, 1135, 1146
BstDSI CCRYGG 5 cut(s) 215, 591, 943, 1011, 1140
BstEII GGTNACC 1 cut(s) 1016
BstFNI CGCG 1 cut(s) 767
BstHHI GCGC 1 cut(s) 397
BstKTI GATC 7 cut(s) 25, 299, 554, 929, 942, 1213, 1282
BstMAI GTCTC 2 cut(s) 888, 1065
BstMBI GATC 7 cut(s) 22, 296, 551, 926, 939, 1210, 1279
BstMWI GCNNNNNNNGC 4 cut(s) 182, 403, 1103, 1151
BstPI GGTNACC 1 cut(s) 1016
BstSFI CTRYAG 1 cut(s) 1033
BstSNI TACGTA 1 cut(s) 1287
BstUI CGCG 1 cut(s) 767
BstV1I GCAGC 3 cut(s) 252, 556, 1305
BstX2I RGATCY 1 cut(s) 926
BstYI RGATCY 1 cut(s) 926
Bsu36I CCTNAGG 1 cut(s) 666
BsuRI GGCC 1 cut(s) 957
BtgI CCRYGG 5 cut(s) 215, 591, 943, 1011, 1140
BtrI CACGTC 1 cut(s) 1119
BtsIMutI CAGTG 5 cut(s) 103, 472, 835, 1202, 1259
Cac8I GCNNGC 2 cut(s) 326, 1152
CciI TCATGA 1 cut(s) 450
CfoI GCGC 1 cut(s) 397
Csp6I GTAC 5 cut(s) 506, 780, 1163, 1284, 1288
CspCI CAANNNNNGTGG 2 cut(s) 790, 825
CviAII CATG 9 cut(s) 118, 216, 451, 469, 548, 592, 984, 1090, 1141
CviQI GTAC 5 cut(s) 506, 780, 1163, 1284, 1288
DdeI CTNAG 7 cut(s) 143, 407, 666, 903, 1006, 1135, 1146
DpnI GATC 7 cut(s) 24, 298, 553, 928, 941, 1212, 1281
DpnII GATC 7 cut(s) 22, 296, 551, 926, 939, 1210, 1279
DraIII CACNNNGTG 2 cut(s) 442, 743
DrdI GACNNNNNNGTC 1 cut(s) 782
DseDI GACNNNNNNGTC 1 cut(s) 782
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco105I TACGTA 1 cut(s) 1287
Eco130I CCWWGG 4 cut(s) 215, 591, 631, 1140
Eco24I GRGCYC 1 cut(s) 75
Eco81I CCTNAGG 1 cut(s) 666
Eco91I GGTNACC 1 cut(s) 1016
EcoO65I GGTNACC 1 cut(s) 1016
EcoT14I CCWWGG 4 cut(s) 215, 591, 631, 1140
EcoT38I GRGCYC 1 cut(s) 75
ErhI CCWWGG 4 cut(s) 215, 591, 631, 1140
FaeI CATG 9 cut(s) 121, 219, 454, 472, 551, 595, 987, 1093, 1144
FalI AAGNNNNNCTT 6 cut(s) 308, 340, 492, 524, 624, 656
FaqI GGGAC 1 cut(s) 1058
FatI CATG 9 cut(s) 117, 215, 450, 468, 547, 591, 983, 1089, 1140
FauI CCCGC 2 cut(s) 613, 1222
FblI GTMKAC 1 cut(s) 1036
Fnu4HI GCNGC 3 cut(s) 266, 545, 1294
FriOI GRGCYC 1 cut(s) 75
Fsp4HI GCNGC 3 cut(s) 266, 545, 1294
FspBI CTAG 5 cut(s) 186, 741, 878, 890, 914
GlaI GCGC 1 cut(s) 396
GluI GCNGC 3 cut(s) 266, 545, 1294
HaeIII GGCC 1 cut(s) 957
HapII CCGG 2 cut(s) 787, 924
HhaI GCGC 1 cut(s) 397
Hin1II CATG 9 cut(s) 121, 219, 454, 472, 551, 595, 987, 1093, 1144
Hin6I GCGC 1 cut(s) 395
HinP1I GCGC 1 cut(s) 395
HincII GTYRAC 1 cut(s) 724
HindII GTYRAC 1 cut(s) 724
HinfI GANTC 9 cut(s) 101, 107, 122, 211, 286, 576, 948, 1233, 1337
HpaII CCGG 2 cut(s) 787, 924
HphI GGTGA 2 cut(s) 85, 703
Hpy166II GTNNAC 5 cut(s) 387, 724, 1037, 1116, 1165
Hpy188I TCNGA 7 cut(s) 22, 106, 112, 127, 1103, 1123, 1349
Hpy8I GTNNAC 5 cut(s) 387, 724, 1037, 1116, 1165
HpyAV CCTTC 2 cut(s) 543, 947
HpyCH4III ACNGT 9 cut(s) 251, 564, 779, 784, 899, 998, 1131, 1193, 1263
HpyCH4IV ACGT 3 cut(s) 1047, 1118, 1286
HpyCH4V TGCA 5 cut(s) 268, 328, 472, 490, 681
HpyF10VI GCNNNNNNNGC 4 cut(s) 182, 403, 1103, 1151
HpyF3I CTNAG 7 cut(s) 143, 407, 666, 903, 1006, 1135, 1146
HpySE526I ACGT 3 cut(s) 1047, 1118, 1286
Hsp92II CATG 9 cut(s) 121, 219, 454, 472, 551, 595, 987, 1093, 1144
HspAI GCGC 1 cut(s) 395
Kzo9I GATC 7 cut(s) 22, 296, 551, 926, 939, 1210, 1279
LguI GCTCTTC 1 cut(s) 300
LmnI GCTCC 1 cut(s) 1094
Lsp1109I GCAGC 3 cut(s) 252, 556, 1305
LweI GCATC 1 cut(s) 787
MaeI CTAG 5 cut(s) 186, 741, 878, 890, 914
MaeII ACGT 3 cut(s) 1047, 1118, 1286
MaeIII GTNAC 4 cut(s) 91, 558, 1016, 1187
MalI GATC 7 cut(s) 24, 298, 553, 928, 941, 1212, 1281
MboI GATC 7 cut(s) 22, 296, 551, 926, 939, 1210, 1279
MboII GAAGA 1 cut(s) 317
MflI RGATCY 1 cut(s) 926
MhlI GDGCHC 1 cut(s) 75
MluCI AATT 3 cut(s) 44, 229, 715
MlyI GAGTC 5 cut(s) 110, 116, 295, 585, 942
MmeI TCCRAC 1 cut(s) 1281
MnlI CCTC 7 cut(s) 76, 231, 402, 617, 661, 1104, 1249
MseI TTAA 4 cut(s) 510, 600, 752, 1308
MslI CAYNNNNRTG 2 cut(s) 982, 1329
MspA1I CMGCKG 3 cut(s) 406, 698, 1154
MspI CCGG 2 cut(s) 787, 924
Mva1269I GAATGC 1 cut(s) 326
MvnI CGCG 1 cut(s) 767
MwoI GCNNNNNNNGC 4 cut(s) 182, 403, 1103, 1151
NcoI CCATGG 3 cut(s) 215, 591, 1140
NdeII GATC 7 cut(s) 22, 296, 551, 926, 939, 1210, 1279
NlaIII CATG 9 cut(s) 121, 219, 454, 472, 551, 595, 987, 1093, 1144
NlaIV GGNNCC 3 cut(s) 629, 989, 1055
NmuCI GTSAC 2 cut(s) 91, 558
PagI TCATGA 1 cut(s) 450
PciSI GCTCTTC 1 cut(s) 300
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PctI GAATGC 1 cut(s) 326
PfeI GAWTC 4 cut(s) 107, 211, 1233, 1337
Pfl23II CGTACG 2 cut(s) 1283, 1287
PkrI GCNGC 3 cut(s) 267, 546, 1295
PleI GAGTC 5 cut(s) 109, 116, 294, 584, 942
PpsI GAGTC 5 cut(s) 109, 116, 294, 584, 942
Ppu21I YACGTR 1 cut(s) 1287
PspEI GGTNACC 1 cut(s) 1016
PspLI CGTACG 2 cut(s) 1283, 1287
PspN4I GGNNCC 3 cut(s) 629, 989, 1055
PsuI RGATCY 1 cut(s) 926
PvuII CAGCTG 2 cut(s) 406, 698
RsaI GTAC 5 cut(s) 507, 781, 1164, 1285, 1289
RsaNI GTAC 5 cut(s) 506, 780, 1163, 1284, 1288
RseI CAYNNNNRTG 2 cut(s) 982, 1329
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 4 cut(s) 510, 600, 752, 1308
SatI GCNGC 3 cut(s) 266, 545, 1294
Sau3AI GATC 7 cut(s) 22, 296, 551, 926, 939, 1210, 1279
ScaI AGTACT 1 cut(s) 507
SchI GAGTC 5 cut(s) 110, 116, 295, 585, 942
SduI GDGCHC 1 cut(s) 75
SfaNI GCATC 1 cut(s) 787
SfcI CTRYAG 1 cut(s) 1033
SmiMI CAYNNNNRTG 2 cut(s) 982, 1329
SmlI CTYRAG 1 cut(s) 1108
SmoI CTYRAG 1 cut(s) 1108
SnaBI TACGTA 1 cut(s) 1287
Sse9I AATT 3 cut(s) 44, 229, 715
SsiI CCGC 4 cut(s) 620, 765, 1154, 1229
SspMI CTAG 5 cut(s) 186, 741, 878, 890, 914
StyI CCWWGG 4 cut(s) 215, 591, 631, 1140
TaaI ACNGT 9 cut(s) 251, 564, 779, 784, 899, 998, 1131, 1193, 1263
TaiI ACGT 3 cut(s) 1050, 1121, 1289
TaqI TCGA 6 cut(s) 12, 60, 138, 295, 951, 963
TasI AATT 3 cut(s) 44, 229, 715
TatI WGTACW 3 cut(s) 505, 779, 1162
TfiI GAWTC 4 cut(s) 107, 211, 1233, 1337
Tru1I TTAA 4 cut(s) 510, 600, 752, 1308
Tru9I TTAA 4 cut(s) 510, 600, 752, 1308
TscAI CASTG 5 cut(s) 103, 479, 842, 1202, 1266
TseFI GTSAC 2 cut(s) 91, 558
TseI GCWGC 3 cut(s) 265, 544, 1293
Tsp45I GTSAC 2 cut(s) 91, 558
TspDTI ATGAA 7 cut(s) 57, 106, 180, 439, 467, 554, 966
TspGWI ACGGA 3 cut(s) 960, 1193, 1268
TspRI CASTG 5 cut(s) 103, 479, 842, 1202, 1266
XapI RAATTY 1 cut(s) 44
XmiI GTMKAC 1 cut(s) 1036
XspI CTAG 5 cut(s) 186, 741, 878, 890, 914
ZrmI AGTACT 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.