MD15G1365300.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001698
Physical Location & Seq
Forward (+)
44268158 .. 44273226
5069 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1365300.v1.1.491

Sequence Viewer

Length: 1461 bp
ATGGCGCAATTTCAGAACGACGAGCTGGAGTACGTCGTCGACGACTACTTCGACATCTCCGACTTCGAAGACACCGACGCTTTTGCCGGTAACCAGCTACGGGGAAGCCACGGCGCCGACTCGGACTTTGACGACGACGACTTCGCTGAGAGCAAGGAGAAGACTGATACTTCGGCAATGGAAGCTCGAAATGGGAAGGACATACAGGGGATTCCGTGGGAGCGGCTTAACTTCACCAGAGATAGGTACCGTGAGACCCGATTGAAGCAGTACAAGAACTACGAAAGCCTCTCTCTGCCTCGCCAAGACCTCGAAAAGGAGTGCTTGCCAGTGGAGATGGGGAAGACCTTCTATGACTTTCACTACAATACAAGGATTGTCAAGTCCACAATTGTGCATTTCCAGGGTGTCTTATTTTATGATTGTGGTTATGTCCCTCAACCATTCACAGGAAGCTTTTACAGCAGGGTGGAAGTTAGAGCTGCTGGCGCGACATTGTTAATATGTGGTCCTTTTCTGAGGAATCTGTTATGGGCCACATCAAAGCACGATGTATACCTTATGCAGAACTTTTCAGTAATGCATTGGTCTTCCCTACTCAGGAGAGGCAAAGAAGTATTGAACGTATCAAAACCAGTTGTCCCGACTGAGAAACGAGCTGGGTTGTTGGTTCGTCCACTCTCTAGAGTGCAAATAAGCACTATGGCTGTGAAGGAAAATTTATTGGTTGCTGGGGGATTCCAAGGTGAACTAATCTGCAAGCACATTAATCAACCTGGAGTTGCTTTCTGCTCAAAATTAACAACAGATGACAATGCCATCACAAATGCAGTGGACATTTTCCAAAACCCTTCTGGCTCAATGAGGGTAATGACTGCAAACAATGATGCTCAAGTTAGAATTTTTGATGTCCAAAATTTCACTTGCCTCAGTCACTTCTCTTTTGATTGGTCCGTGAATAACACCTCTGTCAGTCCAGATGGTAAGCTGGTTGCAATACTCGGAGATAGTGCGGACTGCTTGATTGCTGATGCTCAGTCTGGAAAAGCTGTCGGAAGCCTCAAGGGTCATTTGGACTATTGCTTTTCCTCAGCATGGCATCCGAACGGACAAATTTTGGCTACCGGGAATCAAGACACCACTTGCAGGTTATGGGACATAAGGAACCTCTCAGAGTCGCTAGCCGTGTTGAAGGGAAGAATGGGAGCAATACGGGCCATAAAGTTCACTTCGGATGGTCGGTTTATGGCCATGGCTGAGCCGGCAGACTTTGTTCACATCCTTGACACCCAATCTGGGTACCTCAAGGAACAAGAGATAGACCTGTTCGGTGAAATCGCAGGAATATCGTTTAGCCCCGACACAGAAGCTCTGTTTGTTGGGGTCGCTGATCGGACGTATGGTAGCGTGTTAGAATTCAACAAGAGGCATCACAACCGATATCTCGAAGCAGTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

487

Amino Acids

54.58

Weight (kDa)

5.31

Isoelectric Point (pI)

31.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 236 - 451 3.4e-19 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 275 - 389 3.7e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 282 - 458 2.2e-25 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 285 - 466 5.3e-21 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 287 - 419 6.3e-11 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 287 - 392 1.6e-07 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 288 - 394 2.1e-07 WDR3 second beta-propeller domain
Beta-prop_EML_2 PF23414 289 - 433 1.2e-14 Echinoderm microtubule-associated protein second beta-propeller
WDR55 PF24796 290 - 462 3.7e-07 WDR55
Beta-prop_WDR3_1st PF25173 295 - 466 1.7e-13 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 322 - 454 2.3e-09 CDC20/Fizzy WD40 domain
WD40 PF00400 349 - 386 8.3e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1137
Acc65I GGTACC 2 cut(s) 246, 1299
AccB1I GGYRCC 3 cut(s) 113, 246, 1299
AccBSI CCGCTC 1 cut(s) 223
AccI GTMKAC 2 cut(s) 39, 555
AccII CGCG 1 cut(s) 491
AciI CCGC 2 cut(s) 223, 1013
AcoI YGGCCR 1 cut(s) 1248
AcsI RAATTY 5 cut(s) 718, 900, 916, 1113, 1415
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 4 cut(s) 32, 248, 272, 1301
AfiI CCNNNNNNNGG 8 cut(s) 100, 243, 316, 449, 600, 1095, 1146, 1296
AgsI TTSAA 4 cut(s) 265, 622, 1192, 1420
AjnI CCWGG 2 cut(s) 402, 775
AleI CACNNNNGTG 1 cut(s) 392
AluBI AGCT 9 cut(s) 25, 97, 185, 456, 482, 659, 988, 1049, 1370
AluI AGCT 9 cut(s) 25, 97, 185, 456, 482, 659, 988, 1049, 1370
Alw26I GTCTC 1 cut(s) 248
AoxI GGCC 3 cut(s) 534, 1215, 1248
ApeKI GCWGC 1 cut(s) 482
ApoI RAATTY 5 cut(s) 718, 900, 916, 1113, 1415
ArsI GACNNNNNNTTYG 8 cut(s) 32, 64, 110, 125, 142, 154, 157, 186
AseI ATTAAT 1 cut(s) 768
Asp700I GAANNNNTTC 1 cut(s) 347
Asp718I GGTACC 2 cut(s) 246, 1299
AspLEI GCGC 3 cut(s) 7, 116, 491
AspS9I GGNCC 4 cut(s) 509, 534, 951, 1215
AsuC2I CCSGG 1 cut(s) 1126
AsuHPI GGTGA 3 cut(s) 226, 758, 1343
AsuII TTCGAA 1 cut(s) 66
AsuNHI GCTAGC 1 cut(s) 1180
AvaII GGWCC 2 cut(s) 509, 951
BalI TGGCCA 1 cut(s) 1250
BanI GGYRCC 3 cut(s) 113, 246, 1299
BbsI GAAGAC 5 cut(s) 75, 167, 350, 582, 1447
BbvCI CCTCAGC 1 cut(s) 1090
BbvI GCAGC 1 cut(s) 469
BccI CCATC 4 cut(s) 331, 827, 974, 1229
BceAI ACGGC 2 cut(s) 127, 1169
BcgI CGANNNNNNTGC 4 cut(s) 65, 99, 1329, 1363
BciT130I CCWGG 2 cut(s) 404, 777
BcnI CCSGG 1 cut(s) 1126
BcoDI GTCTC 1 cut(s) 248
BfaI CTAG 3 cut(s) 684, 1181, 1459
BfoI RGCGCY 1 cut(s) 117
BfuAI ACCTGC 1 cut(s) 1137
BisI GCNGC 2 cut(s) 224, 483
BlpI GCTNAGC 1 cut(s) 1257
BlsI GCNGC 2 cut(s) 225, 484
Bme1390I CCNGG 3 cut(s) 404, 777, 1126
Bme18I GGWCC 2 cut(s) 509, 951
BmgT120I GGNCC 4 cut(s) 509, 534, 951, 1215
BmiI GGNNCC 4 cut(s) 115, 248, 1166, 1301
BmrFI CCNGG 3 cut(s) 404, 777, 1126
BmsI GCATC 4 cut(s) 877, 1021, 1108, 1438
BmtI GCTAGC 1 cut(s) 1184
BpiI GAAGAC 5 cut(s) 75, 167, 350, 582, 1447
BpmI CTGGAG 2 cut(s) 47, 798
Bpu10I CCTNAGC 1 cut(s) 1090
Bpu1102I GCTNAGC 1 cut(s) 1257
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 3 cut(s) 876, 1046, 1289
BpuMI CCSGG 1 cut(s) 1126
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 248
BsaJI CCNNGG 5 cut(s) 109, 215, 403, 742, 1251
Bsc4I CCNNNNNNNGG 8 cut(s) 100, 243, 316, 449, 600, 1095, 1146, 1296
Bse118I RCCGGY 2 cut(s) 86, 1261
Bse1I ACTGG 2 cut(s) 329, 635
Bse3DI GCAATG 1 cut(s) 183
BseBI CCWGG 2 cut(s) 404, 777
BseDI CCNNGG 5 cut(s) 109, 215, 403, 742, 1251
BseGI GGATG 3 cut(s) 1099, 1240, 1278
BseLI CCNNNNNNNGG 8 cut(s) 100, 243, 316, 449, 600, 1095, 1146, 1296
BseMI GCAATG 1 cut(s) 183
BseMII CTCAG 9 cut(s) 138, 509, 613, 639, 943, 1049, 1104, 1185, 1248
BseNI ACTGG 2 cut(s) 329, 635
BseXI GCAGC 1 cut(s) 469
BseYI CCCAGC 2 cut(s) 659, 731
Bsh1236I CGCG 1 cut(s) 491
BshFI GGCC 3 cut(s) 536, 1217, 1250
BshNI GGYRCC 3 cut(s) 113, 246, 1299
BsiSI CCGG 3 cut(s) 87, 1125, 1262
BslFI GGGAC 3 cut(s) 419, 626, 1169
BslI CCNNNNNNNGG 8 cut(s) 100, 243, 316, 449, 600, 1095, 1146, 1296
BsmAI GTCTC 1 cut(s) 248
BsmFI GGGAC 3 cut(s) 419, 626, 1169
BsnI GGCC 3 cut(s) 536, 1217, 1250
Bso31I GGTCTC 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 66
Bsp143I GATC 1 cut(s) 1390
Bsp1720I GCTNAGC 1 cut(s) 1257
Bsp19I CCATGG 1 cut(s) 1251
BspACI CCGC 2 cut(s) 223, 1013
BspANI GGCC 3 cut(s) 536, 1217, 1250
BspCNI CTCAG 9 cut(s) 139, 510, 612, 640, 942, 1048, 1103, 1184, 1249
BspFNI CGCG 1 cut(s) 491
BspLI GGNNCC 4 cut(s) 115, 248, 1166, 1301
BspMI ACCTGC 1 cut(s) 1137
BspOI GCTAGC 1 cut(s) 1184
BspT104I TTCGAA 1 cut(s) 66
BspT107I GGYRCC 3 cut(s) 113, 246, 1299
BspTNI GGTCTC 1 cut(s) 248
BsrBI CCGCTC 1 cut(s) 223
BsrDI GCAATG 1 cut(s) 183
BsrFI RCCGGY 2 cut(s) 86, 1261
BsrI ACTGG 2 cut(s) 329, 635
BssAI RCCGGY 2 cut(s) 86, 1261
BssECI CCNNGG 5 cut(s) 109, 215, 403, 742, 1251
BssMI GATC 1 cut(s) 1390
BssNAI GTATAC 1 cut(s) 556
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 742, 1251
Bst1107I GTATAC 1 cut(s) 556
Bst2UI CCWGG 2 cut(s) 404, 777
Bst4CI ACNGT 1 cut(s) 251
BstACI GRCGYC 1 cut(s) 114
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 5 cut(s) 326, 487, 761, 1182, 1263
BstDEI CTNAG 9 cut(s) 147, 518, 599, 648, 929, 1035, 1090, 1171, 1257
BstDSI CCRYGG 3 cut(s) 109, 215, 1251
BstEII GGTNACC 1 cut(s) 89
BstENI CCTNNNNNAGG 1 cut(s) 314
BstF5I GGATG 3 cut(s) 1099, 1240, 1278
BstFNI CGCG 1 cut(s) 491
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 3 cut(s) 7, 116, 491
BstKTI GATC 1 cut(s) 1393
BstMAI GTCTC 1 cut(s) 248
BstMBI GATC 1 cut(s) 1390
BstMWI GCNNNNNNNGC 5 cut(s) 182, 462, 488, 1214, 1262
BstNI CCWGG 2 cut(s) 404, 777
BstPI GGTNACC 1 cut(s) 89
BstSCI CCNGG 3 cut(s) 402, 775, 1124
BstUI CGCG 1 cut(s) 491
BstV1I GCAGC 1 cut(s) 469
BstV2I GAAGAC 5 cut(s) 75, 167, 350, 582, 1447
BstZ17I GTATAC 1 cut(s) 556
BsuRI GGCC 3 cut(s) 536, 1217, 1250
BtgI CCRYGG 3 cut(s) 109, 215, 1251
BtsCI GGATG 3 cut(s) 1099, 1240, 1278
BtsI GCAGTG 1 cut(s) 837
BtsIMutI CAGTG 2 cut(s) 336, 837
BveI ACCTGC 1 cut(s) 1137
Cac8I GCNNGC 5 cut(s) 326, 487, 761, 1182, 1263
CfoI GCGC 3 cut(s) 7, 116, 491
Cfr10I RCCGGY 2 cut(s) 86, 1261
Cfr13I GGNCC 4 cut(s) 509, 534, 951, 1215
CseI GACGC 1 cut(s) 86
Csp6I GTAC 4 cut(s) 31, 247, 271, 1300
CspCI CAANNNNNGTGG 2 cut(s) 813, 848
CviAII CATG 2 cut(s) 1095, 1252
CviQI GTAC 4 cut(s) 31, 247, 271, 1300
DdeI CTNAG 9 cut(s) 147, 518, 599, 648, 929, 1035, 1090, 1171, 1257
DinI GGCGCC 1 cut(s) 115
DpnI GATC 1 cut(s) 1392
DpnII GATC 1 cut(s) 1390
EaeI YGGCCR 1 cut(s) 1248
Eco130I CCWWGG 2 cut(s) 742, 1251
Eco31I GGTCTC 1 cut(s) 248
Eco32I GATATC 1 cut(s) 1442
Eco47I GGWCC 2 cut(s) 509, 951
Eco91I GGTNACC 1 cut(s) 89
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoO65I GGTNACC 1 cut(s) 89
EcoRI GAATTC 1 cut(s) 1415
EcoRII CCWGG 2 cut(s) 402, 775
EcoRV GATATC 1 cut(s) 1442
EcoT14I CCWWGG 2 cut(s) 742, 1251
EcoT22I ATGCAT 1 cut(s) 585
EgeI GGCGCC 1 cut(s) 115
EheI GGCGCC 1 cut(s) 115
ErhI CCWWGG 2 cut(s) 742, 1251
FaeI CATG 2 cut(s) 1098, 1255
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FaqI GGGAC 3 cut(s) 419, 626, 1169
FatI CATG 2 cut(s) 1094, 1251
FblI GTMKAC 2 cut(s) 39, 555
Fnu4HI GCNGC 2 cut(s) 224, 483
FokI GGATG 3 cut(s) 1086, 1247, 1265
Fsp4HI GCNGC 2 cut(s) 224, 483
FspBI CTAG 3 cut(s) 684, 1181, 1459
GlaI GCGC 3 cut(s) 6, 115, 490
GluI GCNGC 2 cut(s) 224, 483
GsaI CCCAGC 2 cut(s) 663, 735
GsuI CTGGAG 2 cut(s) 47, 798
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 3 cut(s) 536, 1217, 1250
HapII CCGG 3 cut(s) 87, 1125, 1262
HgaI GACGC 1 cut(s) 86
HhaI GCGC 3 cut(s) 7, 116, 491
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 2 cut(s) 1098, 1255
Hin6I GCGC 3 cut(s) 5, 114, 489
HinP1I GCGC 3 cut(s) 5, 114, 489
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 454
HinfI GANTC 6 cut(s) 119, 211, 523, 738, 1129, 1175
HpaII CCGG 3 cut(s) 87, 1125, 1262
HphI GGTGA 3 cut(s) 226, 758, 1343
Hpy166II GTNNAC 8 cut(s) 40, 387, 556, 677, 749, 835, 1227, 1276
Hpy188III TCNNGA 7 cut(s) 601, 643, 684, 977, 1041, 1133, 1445
Hpy8I GTNNAC 8 cut(s) 40, 387, 556, 677, 749, 835, 1227, 1276
Hpy99I CGWCG 7 cut(s) 23, 38, 41, 44, 80, 137, 140
HpyAV CCTTC 5 cut(s) 190, 358, 706, 861, 1186
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4IV ACGT 3 cut(s) 33, 624, 1397
HpyCH4V TGCA 9 cut(s) 397, 565, 583, 691, 759, 830, 878, 995, 1146
HpyF10VI GCNNNNNNNGC 5 cut(s) 182, 462, 488, 1214, 1262
HpyF3I CTNAG 9 cut(s) 147, 518, 599, 648, 929, 1035, 1090, 1171, 1257
HpySE526I ACGT 3 cut(s) 33, 624, 1397
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 2 cut(s) 1098, 1255
HspAI GCGC 3 cut(s) 5, 114, 489
KasI GGCGCC 1 cut(s) 113
KpnI GGTACC 2 cut(s) 250, 1303
KroI GCCGGC 1 cut(s) 1261
KroNI GCCGGC 1 cut(s) 1263
Kzo9I GATC 1 cut(s) 1390
LmnI GCTCC 2 cut(s) 220, 1205
Lsp1109I GCAGC 1 cut(s) 469
LweI GCATC 4 cut(s) 877, 1021, 1108, 1438
MaeI CTAG 3 cut(s) 684, 1181, 1459
MaeII ACGT 3 cut(s) 33, 624, 1397
MaeIII GTNAC 2 cut(s) 89, 932
MalI GATC 1 cut(s) 1392
MbiI CCGCTC 1 cut(s) 223
MboI GATC 1 cut(s) 1390
MboII GAAGA 6 cut(s) 80, 172, 355, 582, 1209, 1447
MfeI CAATTG 1 cut(s) 390
MlsI TGGCCA 1 cut(s) 1250
MluCI AATT 8 cut(s) 8, 390, 718, 797, 900, 916, 1113, 1415
MluNI TGGCCA 1 cut(s) 1250
Mly113I GGCGCC 1 cut(s) 114
MlyI GAGTC 2 cut(s) 113, 1184
MmeI TCCRAC 2 cut(s) 84, 1033
Mox20I TGGCCA 1 cut(s) 1250
Mph1103I ATGCAT 1 cut(s) 585
MroNI GCCGGC 1 cut(s) 1261
MroXI GAANNNNTTC 1 cut(s) 347
MscI TGGCCA 1 cut(s) 1250
MseI TTAA 4 cut(s) 228, 500, 768, 800
MslI CAYNNNNRTG 1 cut(s) 392
Msp20I TGGCCA 1 cut(s) 1250
MspI CCGG 3 cut(s) 87, 1125, 1262
MspR9I CCNGG 3 cut(s) 404, 777, 1126
MunI CAATTG 1 cut(s) 390
MvaI CCWGG 2 cut(s) 404, 777
MvnI CGCG 1 cut(s) 491
MwoI GCNNNNNNNGC 5 cut(s) 182, 462, 488, 1214, 1262
NaeI GCCGGC 1 cut(s) 1263
NarI GGCGCC 1 cut(s) 114
NciI CCSGG 1 cut(s) 1126
NcoI CCATGG 1 cut(s) 1251
NdeII GATC 1 cut(s) 1390
NgoMIV GCCGGC 1 cut(s) 1261
NheI GCTAGC 1 cut(s) 1180
NlaIII CATG 2 cut(s) 1098, 1255
NlaIV GGNNCC 4 cut(s) 115, 248, 1166, 1301
NmuCI GTSAC 1 cut(s) 932
NsiI ATGCAT 1 cut(s) 585
NspV TTCGAA 1 cut(s) 66
OliI CACNNNNGTG 1 cut(s) 392
PcsI WCGNNNNNNNCGW 4 cut(s) 39, 48, 57, 72
PdiI GCCGGC 1 cut(s) 1263
PdmI GAANNNNTTC 1 cut(s) 347
PfeI GAWTC 4 cut(s) 211, 523, 738, 1129
PkrI GCNGC 2 cut(s) 225, 484
PleI GAGTC 2 cut(s) 113, 1183
PluTI GGCGCC 1 cut(s) 117
PpsI GAGTC 2 cut(s) 113, 1183
PshBI ATTAAT 1 cut(s) 768
Psp6I CCWGG 2 cut(s) 402, 775
PspEI GGTNACC 1 cut(s) 89
PspFI CCCAGC 2 cut(s) 659, 731
PspGI CCWGG 2 cut(s) 402, 775
PspN4I GGNNCC 4 cut(s) 115, 248, 1166, 1301
PspPI GGNCC 4 cut(s) 509, 534, 951, 1215
RsaI GTAC 4 cut(s) 32, 248, 272, 1301
RsaNI GTAC 4 cut(s) 31, 247, 271, 1300
RseI CAYNNNNRTG 1 cut(s) 392
SalI GTCGAC 1 cut(s) 38
SaqAI TTAA 4 cut(s) 228, 500, 768, 800
SatI GCNGC 2 cut(s) 224, 483
Sau3AI GATC 1 cut(s) 1390
Sau96I GGNCC 4 cut(s) 509, 534, 951, 1215
SchI GAGTC 2 cut(s) 113, 1184
ScrFI CCNGG 3 cut(s) 404, 777, 1126
SfaNI GCATC 4 cut(s) 877, 1021, 1108, 1438
SfoI GGCGCC 1 cut(s) 115
SfuI TTCGAA 1 cut(s) 66
SgrDI CGTCGACG 1 cut(s) 38
SinI GGWCC 2 cut(s) 509, 951
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 3 cut(s) 891, 1061, 1304
SmoI CTYRAG 3 cut(s) 891, 1061, 1304
Sse9I AATT 8 cut(s) 8, 390, 718, 797, 900, 916, 1113, 1415
SsiI CCGC 2 cut(s) 223, 1013
SspDI GGCGCC 1 cut(s) 113
SspMI CTAG 3 cut(s) 684, 1181, 1459
StyD4I CCNGG 3 cut(s) 402, 775, 1124
StyI CCWWGG 2 cut(s) 742, 1251
TaaI ACNGT 1 cut(s) 251
TaiI ACGT 3 cut(s) 36, 627, 1400
TaqI TCGA 6 cut(s) 39, 51, 66, 187, 312, 1446
TasI AATT 8 cut(s) 8, 390, 718, 797, 900, 916, 1113, 1415
TatI WGTACW 1 cut(s) 270
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 4 cut(s) 211, 523, 738, 1129
Tru1I TTAA 4 cut(s) 228, 500, 768, 800
Tru9I TTAA 4 cut(s) 228, 500, 768, 800
TscAI CASTG 2 cut(s) 336, 837
TseFI GTSAC 1 cut(s) 932
TseI GCWGC 1 cut(s) 482
Tsp45I GTSAC 1 cut(s) 932
TspGWI ACGGA 3 cut(s) 204, 943, 1122
TspRI CASTG 2 cut(s) 336, 837
VpaK11BI GGWCC 2 cut(s) 509, 951
VspI ATTAAT 1 cut(s) 768
XagI CCTNNNNNAGG 1 cut(s) 314
XapI RAATTY 5 cut(s) 718, 900, 916, 1113, 1415
XbaI TCTAGA 1 cut(s) 683
XcmI CCANNNNNNNNNTGG 1 cut(s) 851
XmiI GTMKAC 2 cut(s) 39, 555
XmnI GAANNNNTTC 1 cut(s) 347
XspI CTAG 3 cut(s) 684, 1181, 1459
Zsp2I ATGCAT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.