RchiOBHm_Chr7g0239781

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001698
Physical Location & Seq
Reverse (-)
65525926 .. 65541812
15887 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21491

Sequence Viewer

Length: 891 bp
ATGTTCTTACAGAAACAACCTGGGTTGTTGGCACGACCACTCTCTAGAGTGCAGATGAGCACTACGGCTCTCAAGGACAATTTGTTGGTTGCTGGGGGCTTCCAAGGTGAACTAATCTGCAAGCACATGAAACAACCTGGAGTTGCTTTCTGCTCAAAACTAACGACAGATGAGAATGCCATCACTAATGCAGTGGATATTTTCCAAAACCCAACTGGCTCAGTGAGGAACACGTCTGTTAGTCCCGATGGTAAGCTTGTAAGCTGCTTGCAGTACTTGGTGATGCTCAGTCTGGAAAAGCTGAGCAGAGCAATCACTGGGAAGTTTGAGAGAAGCACAGGTTTGCTAAAGCGTGACTTTAGCTTCGCTGGGTGCGAGGGCGTTACCCTTGCTTCGCTGGAAGTAACAGTGGCGGTTGTGCTAGCAGTCGGCTCGCGAGGAGACTGGGACTGGAAGTACGGTCGCCGGGTTGATCTGGCTGTCGGGGGCCTCAAAGGGCACTTAGACTATTGCTTTTCTTCAGCTTGGCACCCGGATGGACGAATCTTGGCTACTGGAAACCAAGACACTACCTGTAGGCTATGGGACGTAAGGAACCTGTCAGAGTCCCTAGCCGTGTTGAAGGGAAGAATGGGAGCAATAAGAGCTATAAAATTCAACTCTGATGGTCGTCTTATGGCCATGGCTGAGCCAGCAGACTTTGTCTACATCATTGATACCCAGTCCGGATATGCAAAGGGACAAGAGATTGATCTGTTTGGTGAGATTGCCGGAATATCCTTCAGCCCCGACTCTGAAGCTTTGTTTGTTGGGGTTGCTGATCGCACATATGGTAGTGTATTGGAGTTCAATAAGAGGCACTATAATCCCTATCAAGAAATAGTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

296

Amino Acids

32.41

Weight (kDa)

7.59

Isoelectric Point (pI)

32.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 152 - 269 8.6e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 160 - 276 2.4e-13 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 162 - 268 1.6e-16 THOC3 beta-propeller domain
WD40 PF00400 164 - 196 2.8e-07 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 164 - 275 1.1e-08 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 171 - 268 2.6e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 173 - 241 7.4e-11 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 528
AccI GTMKAC 1 cut(s) 705
AccII CGCG 1 cut(s) 436
AccIII TCCGGA 1 cut(s) 725
AciI CCGC 1 cut(s) 413
AcoI YGGCCR 1 cut(s) 678
AcsI RAATTY 1 cut(s) 653
AcuI CTGAAG 3 cut(s) 504, 766, 816
AfaI GTAC 2 cut(s) 275, 458
AflIII ACRYGT 1 cut(s) 231
AgsI TTSAA 3 cut(s) 622, 658, 850
AjiI CACGTC 1 cut(s) 234
AjnI CCWGG 2 cut(s) 19, 136
AluBI AGCT 7 cut(s) 256, 264, 301, 363, 524, 647, 800
AluI AGCT 7 cut(s) 256, 264, 301, 363, 524, 647, 800
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 435
Aor13HI TCCGGA 1 cut(s) 725
AoxI GGCC 2 cut(s) 487, 678
ApeKI GCWGC 1 cut(s) 264
ApoI RAATTY 1 cut(s) 653
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
AspS9I GGNCC 1 cut(s) 487
AsuC2I CCSGG 2 cut(s) 467, 533
AsuHPI GGTGA 3 cut(s) 119, 292, 773
AsuNHI GCTAGC 1 cut(s) 421
BaeGI GKGCMC 1 cut(s) 501
BalI TGGCCA 1 cut(s) 680
BanI GGYRCC 1 cut(s) 528
BbsI GAAGAC 1 cut(s) 877
Bbv12I GWGCWC 1 cut(s) 62
BbvI GCAGC 1 cut(s) 251
BccI CCATC 4 cut(s) 188, 242, 530, 659
BceAI ACGGC 2 cut(s) 81, 599
BcgI CGANNNNNNTGC 2 cut(s) 414, 448
BciT130I CCWGG 2 cut(s) 21, 138
BcnI CCSGG 2 cut(s) 467, 533
BcoDI GTCTC 1 cut(s) 435
BfaI CTAG 4 cut(s) 45, 422, 611, 889
BfmI CTRYAG 1 cut(s) 574
BisI GCNGC 1 cut(s) 265
BlpI GCTNAGC 2 cut(s) 302, 687
BlsI GCNGC 1 cut(s) 266
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 4 cut(s) 21, 138, 467, 533
BmgBI CACGTC 1 cut(s) 234
BmgT120I GGNCC 1 cut(s) 487
BmiI GGNNCC 3 cut(s) 488, 530, 596
BmrFI CCNGG 4 cut(s) 21, 138, 467, 533
BmrI ACTGGG 3 cut(s) 327, 454, 715
BmsI GCATC 1 cut(s) 273
BmtI GCTAGC 1 cut(s) 425
BmuI ACTGGG 3 cut(s) 327, 454, 715
BpiI GAAGAC 1 cut(s) 877
BpmI CTGGAG 1 cut(s) 159
Bpu1102I GCTNAGC 2 cut(s) 302, 687
BpuEI CTTGAG 1 cut(s) 56
BpuMI CCSGG 2 cut(s) 467, 533
BsaJI CCNNGG 3 cut(s) 20, 103, 681
BsaWI WCCGGW 1 cut(s) 725
Bse1I ACTGG 6 cut(s) 220, 322, 449, 455, 559, 721
BseAI TCCGGA 1 cut(s) 725
BseBI CCWGG 2 cut(s) 21, 138
BseDI CCNNGG 3 cut(s) 20, 103, 681
BseGI GGATG 1 cut(s) 541
BseMII CTCAG 4 cut(s) 234, 293, 301, 678
BseNI ACTGG 6 cut(s) 220, 322, 449, 455, 559, 721
BseRI GAGGAG 1 cut(s) 453
BseSI GKGCMC 1 cut(s) 501
BseXI GCAGC 1 cut(s) 251
BseYI CCCAGC 2 cut(s) 92, 368
BsgI GTGCAG 1 cut(s) 71
Bsh1236I CGCG 1 cut(s) 436
Bsh1285I CGRYCG 1 cut(s) 463
BshFI GGCC 2 cut(s) 489, 680
BshNI GGYRCC 1 cut(s) 528
BsiEI CGRYCG 1 cut(s) 463
BsiHKAI GWGCWC 1 cut(s) 62
BsiSI CCGG 4 cut(s) 466, 533, 726, 771
BslFI GGGAC 5 cut(s) 228, 461, 592, 599, 753
BsmAI GTCTC 1 cut(s) 435
BsmFI GGGAC 5 cut(s) 228, 461, 592, 599, 753
BsmI GAATGC 1 cut(s) 181
BsnI GGCC 2 cut(s) 489, 680
Bsp1286I GDGCHC 2 cut(s) 62, 501
Bsp13I TCCGGA 1 cut(s) 725
Bsp143I GATC 3 cut(s) 472, 751, 820
Bsp1720I GCTNAGC 2 cut(s) 302, 687
Bsp19I CCATGG 1 cut(s) 681
Bsp68I TCGCGA 1 cut(s) 436
BspACI CCGC 1 cut(s) 413
BspANI GGCC 2 cut(s) 489, 680
BspCNI CTCAG 4 cut(s) 233, 294, 300, 679
BspEI TCCGGA 1 cut(s) 725
BspFNI CGCG 1 cut(s) 436
BspLI GGNNCC 3 cut(s) 488, 530, 596
BspOI GCTAGC 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 528
BsrI ACTGG 6 cut(s) 220, 322, 449, 455, 559, 721
BssECI CCNNGG 3 cut(s) 20, 103, 681
BssMI GATC 3 cut(s) 472, 751, 820
BssT1I CCWWGG 2 cut(s) 103, 681
Bst2UI CCWGG 2 cut(s) 21, 138
Bst4CI ACNGT 2 cut(s) 409, 461
BstC8I GCNNGC 5 cut(s) 122, 269, 423, 434, 693
BstDEI CTNAG 5 cut(s) 220, 287, 302, 502, 687
BstDSI CCRYGG 1 cut(s) 681
BstF5I GGATG 1 cut(s) 541
BstFNI CGCG 1 cut(s) 436
BstKTI GATC 3 cut(s) 475, 754, 823
BstMAI GTCTC 1 cut(s) 435
BstMBI GATC 3 cut(s) 472, 751, 820
BstMCI CGRYCG 1 cut(s) 463
BstMWI GCNNNNNNNGC 2 cut(s) 644, 692
BstNI CCWGG 2 cut(s) 21, 138
BstSCI CCNGG 4 cut(s) 19, 136, 465, 531
BstSFI CTRYAG 1 cut(s) 574
BstSLI GKGCMC 1 cut(s) 501
BstUI CGCG 1 cut(s) 436
BstV1I GCAGC 1 cut(s) 251
BstV2I GAAGAC 1 cut(s) 877
BsuRI GGCC 2 cut(s) 489, 680
BtgI CCRYGG 1 cut(s) 681
BtrI CACGTC 1 cut(s) 234
BtsCI GGATG 1 cut(s) 541
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 4 cut(s) 198, 228, 315, 414
BtuMI TCGCGA 1 cut(s) 436
Cac8I GCNNGC 5 cut(s) 122, 269, 423, 434, 693
Cfr13I GGNCC 1 cut(s) 487
Csp6I GTAC 2 cut(s) 274, 457
CviAII CATG 2 cut(s) 127, 682
CviQI GTAC 2 cut(s) 274, 457
DdeI CTNAG 5 cut(s) 220, 287, 302, 502, 687
DpnI GATC 3 cut(s) 474, 753, 822
DpnII GATC 3 cut(s) 472, 751, 820
EaeI YGGCCR 1 cut(s) 678
Eco130I CCWWGG 2 cut(s) 103, 681
Eco57I CTGAAG 3 cut(s) 504, 766, 816
EcoO109I RGGNCCY 1 cut(s) 487
EcoRII CCWGG 2 cut(s) 19, 136
EcoT14I CCWWGG 2 cut(s) 103, 681
ErhI CCWWGG 2 cut(s) 103, 681
FaeI CATG 2 cut(s) 130, 685
FaiI YATR 9 cut(s) 128, 583, 650, 677, 683, 732, 829, 831, 864
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 5 cut(s) 228, 461, 592, 599, 753
FatI CATG 2 cut(s) 126, 681
FauNDI CATATG 1 cut(s) 829
FblI GTMKAC 1 cut(s) 705
Fnu4HI GCNGC 1 cut(s) 265
FokI GGATG 1 cut(s) 548
Fsp4HI GCNGC 1 cut(s) 265
FspBI CTAG 4 cut(s) 45, 422, 611, 889
GluI GCNGC 1 cut(s) 265
GsaI CCCAGC 2 cut(s) 96, 372
GsuI CTGGAG 1 cut(s) 159
HaeIII GGCC 2 cut(s) 489, 680
HapII CCGG 4 cut(s) 466, 533, 726, 771
Hin1II CATG 2 cut(s) 130, 685
HindIII AAGCTT 2 cut(s) 254, 798
HinfI GANTC 3 cut(s) 543, 605, 791
HpaII CCGG 4 cut(s) 466, 533, 726, 771
HphI GGTGA 3 cut(s) 119, 292, 773
Hpy166II GTNNAC 2 cut(s) 110, 706
Hpy188I TCNGA 3 cut(s) 604, 664, 796
Hpy188III TCNNGA 6 cut(s) 45, 245, 293, 435, 726, 875
Hpy8I GTNNAC 2 cut(s) 110, 706
HpyAV CCTTC 2 cut(s) 616, 790
HpyCH4III ACNGT 2 cut(s) 409, 461
HpyCH4IV ACGT 2 cut(s) 233, 588
HpyCH4V TGCA 5 cut(s) 52, 120, 191, 271, 734
HpyF10VI GCNNNNNNNGC 2 cut(s) 644, 692
HpyF3I CTNAG 5 cut(s) 220, 287, 302, 502, 687
HpySE526I ACGT 2 cut(s) 233, 588
Hsp92II CATG 2 cut(s) 130, 685
Kpn2I TCCGGA 1 cut(s) 725
Kzo9I GATC 3 cut(s) 472, 751, 820
LmnI GCTCC 1 cut(s) 635
Lsp1109I GCAGC 1 cut(s) 251
LweI GCATC 1 cut(s) 273
MaeI CTAG 4 cut(s) 45, 422, 611, 889
MaeII ACGT 2 cut(s) 233, 588
MaeIII GTNAC 3 cut(s) 353, 382, 403
MalI GATC 3 cut(s) 474, 753, 822
MboI GATC 3 cut(s) 472, 751, 820
MboII GAAGA 3 cut(s) 510, 639, 877
MhlI GDGCHC 2 cut(s) 62, 501
MlsI TGGCCA 1 cut(s) 680
MluCI AATT 2 cut(s) 79, 653
MluNI TGGCCA 1 cut(s) 680
MlyI GAGTC 2 cut(s) 614, 785
MnlI CCTC 5 cut(s) 219, 370, 431, 500, 849
Mox20I TGGCCA 1 cut(s) 680
MroI TCCGGA 1 cut(s) 725
MscI TGGCCA 1 cut(s) 680
MslI CAYNNNNRTG 1 cut(s) 534
Msp20I TGGCCA 1 cut(s) 680
MspI CCGG 4 cut(s) 466, 533, 726, 771
MspR9I CCNGG 4 cut(s) 21, 138, 467, 533
Mva1269I GAATGC 1 cut(s) 181
MvaI CCWGG 2 cut(s) 21, 138
MvnI CGCG 1 cut(s) 436
MwoI GCNNNNNNNGC 2 cut(s) 644, 692
NciI CCSGG 2 cut(s) 467, 533
NcoI CCATGG 1 cut(s) 681
NdeI CATATG 1 cut(s) 829
NdeII GATC 3 cut(s) 472, 751, 820
NheI GCTAGC 1 cut(s) 421
NlaIII CATG 2 cut(s) 130, 685
NlaIV GGNNCC 3 cut(s) 488, 530, 596
NmuCI GTSAC 1 cut(s) 353
NruI TCGCGA 1 cut(s) 436
PcsI WCGNNNNNNNCGW 1 cut(s) 372
PctI GAATGC 1 cut(s) 181
PfeI GAWTC 1 cut(s) 543
PflFI GACNNNGTC 1 cut(s) 701
PkrI GCNGC 1 cut(s) 266
PleI GAGTC 2 cut(s) 613, 785
PpsI GAGTC 2 cut(s) 613, 785
Psp6I CCWGG 2 cut(s) 19, 136
PspFI CCCAGC 2 cut(s) 92, 368
PspGI CCWGG 2 cut(s) 19, 136
PspN4I GGNNCC 3 cut(s) 488, 530, 596
PspPI GGNCC 1 cut(s) 487
PsyI GACNNNGTC 1 cut(s) 701
RruI TCGCGA 1 cut(s) 436
RsaI GTAC 2 cut(s) 275, 458
RsaNI GTAC 2 cut(s) 274, 457
RseI CAYNNNNRTG 1 cut(s) 534
SatI GCNGC 1 cut(s) 265
Sau3AI GATC 3 cut(s) 472, 751, 820
Sau96I GGNCC 1 cut(s) 487
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 2 cut(s) 614, 785
ScrFI CCNGG 4 cut(s) 21, 138, 467, 533
SduI GDGCHC 2 cut(s) 62, 501
SfaNI GCATC 1 cut(s) 273
SfcI CTRYAG 1 cut(s) 574
SmiMI CAYNNNNRTG 1 cut(s) 534
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
Sse9I AATT 2 cut(s) 79, 653
SsiI CCGC 1 cut(s) 413
SspMI CTAG 4 cut(s) 45, 422, 611, 889
StyD4I CCNGG 4 cut(s) 19, 136, 465, 531
StyI CCWWGG 2 cut(s) 103, 681
TaaI ACNGT 2 cut(s) 409, 461
TaiI ACGT 2 cut(s) 236, 591
TasI AATT 2 cut(s) 79, 653
TatI WGTACW 1 cut(s) 273
TfiI GAWTC 1 cut(s) 543
TscAI CASTG 4 cut(s) 198, 228, 322, 414
TseFI GTSAC 1 cut(s) 353
TseI GCWGC 1 cut(s) 264
Tsp45I GTSAC 1 cut(s) 353
TspDTI ATGAA 1 cut(s) 143
TspRI CASTG 4 cut(s) 198, 228, 322, 414
Tth111I GACNNNGTC 1 cut(s) 701
XapI RAATTY 1 cut(s) 653
XbaI TCTAGA 1 cut(s) 44
XcmI CCANNNNNNNNNTGG 1 cut(s) 212
XmiI GTMKAC 1 cut(s) 705
XspI CTAG 4 cut(s) 45, 422, 611, 889
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.