RLG00000000730

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001698
Physical Location & Seq
Forward (+)
4547536 .. 4551967
4432 bp
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UTR
Exon/CDS
Intron
RLM00000000730

Sequence Viewer

Length: 447 bp
ATGGCGTATTTCCAGAACGATGACCTCGAGTACGCTGTCGGGGGCCTGAAAGGGCACTTAGACTATTGCTTTTCTTCAGCTTGGCACCCGGATGGACGAATCTTGGCTACTGGGAACCAAGACACTACCTGTAGGCTATGGGACATCAGGAACCTGTCAGAGTCCCTAGCCGTGTTGAAGGGAAGAATGGGAGCAATACGAGCTATAAAATTCACTTCTGATGGTCGTTTTATGGCCATGGCTGAGCCAGCAGACTTTGTTCACATCATTGATACCCAGTCCGGATATGCAAAGGGACAAGAGATTGATCTGTTTGGTGAGATTGCTGGAATATCCTTCAGCCCCGACTCTGAAGCTTTGTTTGTTGGGGTTGCTGATCGCACATATGGTAGTGTATTGGAGTTCAATAAGAGGCACTATAATACCTATGAAGAAATAGTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

149

Amino Acids

16.53

Weight (kDa)

4.85

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 14 - 120 2.6e-17 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 14 - 130 1.7e-13 WDR5 beta-propeller domain
WD40 PF00400 15 - 48 9.3e-08 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 16 - 72 5.6e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 16 - 128 5.1e-10 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 16 - 128 1e-06 G protein beta WD-40 repeat protein
Beta-prop_EML_2 PF23414 23 - 119 6.2e-09 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 25 - 95 5.7e-12 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 27 - 122 1.6e-07 CDC20/Fizzy WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 84
AccIII TCCGGA 1 cut(s) 281
AcoI YGGCCR 1 cut(s) 234
AcsI RAATTY 1 cut(s) 209
AcuI CTGAAG 3 cut(s) 60, 322, 372
AfaI GTAC 1 cut(s) 32
AgsI TTSAA 2 cut(s) 178, 406
AluBI AGCT 3 cut(s) 80, 203, 356
AluI AGCT 3 cut(s) 80, 203, 356
Ama87I CYCGRG 1 cut(s) 26
Aor13HI TCCGGA 1 cut(s) 281
AoxI GGCC 2 cut(s) 43, 234
ApoI RAATTY 1 cut(s) 209
AspS9I GGNCC 1 cut(s) 43
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 1 cut(s) 329
AvaI CYCGRG 1 cut(s) 26
BaeGI GKGCMC 1 cut(s) 57
BalI TGGCCA 1 cut(s) 236
BanI GGYRCC 1 cut(s) 84
BbsI GAAGAC 1 cut(s) 433
BccI CCATC 2 cut(s) 86, 215
BceAI ACGGC 1 cut(s) 155
BcnI CCSGG 1 cut(s) 89
BfaI CTAG 2 cut(s) 167, 445
BfmI CTRYAG 1 cut(s) 130
BlpI GCTNAGC 1 cut(s) 243
Bme1390I CCNGG 1 cut(s) 89
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 43
BmiI GGNNCC 4 cut(s) 44, 86, 116, 152
BmrFI CCNGG 1 cut(s) 89
BmrI ACTGGG 2 cut(s) 120, 271
BmuI ACTGGG 2 cut(s) 120, 271
BpiI GAAGAC 1 cut(s) 433
Bpu1102I GCTNAGC 1 cut(s) 243
BpuMI CCSGG 1 cut(s) 89
BsaJI CCNNGG 1 cut(s) 237
BsaWI WCCGGW 1 cut(s) 281
Bse1I ACTGG 2 cut(s) 115, 277
BseAI TCCGGA 1 cut(s) 281
BseDI CCNNGG 1 cut(s) 237
BseGI GGATG 1 cut(s) 97
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 2 cut(s) 115, 277
BseSI GKGCMC 1 cut(s) 57
BshFI GGCC 2 cut(s) 45, 236
BshNI GGYRCC 1 cut(s) 84
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 2 cut(s) 89, 282
BslFI GGGAC 3 cut(s) 148, 155, 309
BsmFI GGGAC 3 cut(s) 148, 155, 309
BsnI GGCC 2 cut(s) 45, 236
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 57
Bsp13I TCCGGA 1 cut(s) 281
Bsp143I GATC 2 cut(s) 307, 376
Bsp1720I GCTNAGC 1 cut(s) 243
Bsp19I CCATGG 1 cut(s) 237
BspANI GGCC 2 cut(s) 45, 236
BspCNI CTCAG 1 cut(s) 235
BspEI TCCGGA 1 cut(s) 281
BspLI GGNNCC 4 cut(s) 44, 86, 116, 152
BspT107I GGYRCC 1 cut(s) 84
BsrI ACTGG 2 cut(s) 115, 277
BssECI CCNNGG 1 cut(s) 237
BssMI GATC 2 cut(s) 307, 376
BssT1I CCWWGG 1 cut(s) 237
BstC8I GCNNGC 1 cut(s) 249
BstDEI CTNAG 2 cut(s) 58, 243
BstDSI CCRYGG 1 cut(s) 237
BstF5I GGATG 1 cut(s) 97
BstKTI GATC 2 cut(s) 310, 379
BstMBI GATC 2 cut(s) 307, 376
BstMWI GCNNNNNNNGC 2 cut(s) 200, 248
BstSCI CCNGG 1 cut(s) 87
BstSFI CTRYAG 1 cut(s) 130
BstSLI GKGCMC 1 cut(s) 57
BstV2I GAAGAC 1 cut(s) 433
BsuRI GGCC 2 cut(s) 45, 236
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 1 cut(s) 97
Cac8I GCNNGC 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 43
Csp6I GTAC 1 cut(s) 31
CviAII CATG 1 cut(s) 238
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 2 cut(s) 58, 243
DpnI GATC 2 cut(s) 309, 378
DpnII GATC 2 cut(s) 307, 376
EaeI YGGCCR 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 237
Eco57I CTGAAG 3 cut(s) 60, 322, 372
Eco88I CYCGRG 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 43
EcoT14I CCWWGG 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 237
FaeI CATG 1 cut(s) 241
FaiI YATR 9 cut(s) 139, 206, 233, 239, 288, 385, 387, 420, 429
FaqI GGGAC 3 cut(s) 148, 155, 309
FatI CATG 1 cut(s) 237
FauNDI CATATG 1 cut(s) 385
FokI GGATG 1 cut(s) 104
FspBI CTAG 2 cut(s) 167, 445
HaeIII GGCC 2 cut(s) 45, 236
HapII CCGG 2 cut(s) 89, 282
Hin1II CATG 1 cut(s) 241
HindIII AAGCTT 1 cut(s) 354
HinfI GANTC 3 cut(s) 99, 161, 347
HpaII CCGG 2 cut(s) 89, 282
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 3 cut(s) 160, 220, 352
Hpy188III TCNNGA 3 cut(s) 13, 148, 282
Hpy8I GTNNAC 1 cut(s) 262
HpyAV CCTTC 2 cut(s) 172, 346
HpyCH4V TGCA 1 cut(s) 290
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 248
HpyF3I CTNAG 2 cut(s) 58, 243
Hsp92II CATG 1 cut(s) 241
Kpn2I TCCGGA 1 cut(s) 281
Kzo9I GATC 2 cut(s) 307, 376
LmnI GCTCC 1 cut(s) 191
MaeI CTAG 2 cut(s) 167, 445
MalI GATC 2 cut(s) 309, 378
MboI GATC 2 cut(s) 307, 376
MboII GAAGA 4 cut(s) 66, 195, 433, 443
MhlI GDGCHC 1 cut(s) 57
MlsI TGGCCA 1 cut(s) 236
MluCI AATT 1 cut(s) 209
MluNI TGGCCA 1 cut(s) 236
MlyI GAGTC 2 cut(s) 170, 341
MnlI CCTC 2 cut(s) 35, 405
Mox20I TGGCCA 1 cut(s) 236
MroI TCCGGA 1 cut(s) 281
MscI TGGCCA 1 cut(s) 236
MslI CAYNNNNRTG 1 cut(s) 90
Msp20I TGGCCA 1 cut(s) 236
MspI CCGG 2 cut(s) 89, 282
MspR9I CCNGG 1 cut(s) 89
MwoI GCNNNNNNNGC 2 cut(s) 200, 248
NciI CCSGG 1 cut(s) 89
NcoI CCATGG 1 cut(s) 237
NdeI CATATG 1 cut(s) 385
NdeII GATC 2 cut(s) 307, 376
NlaIII CATG 1 cut(s) 241
NlaIV GGNNCC 4 cut(s) 44, 86, 116, 152
PaeR7I CTCGAG 1 cut(s) 26
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PfeI GAWTC 1 cut(s) 99
PleI GAGTC 2 cut(s) 169, 341
PpsI GAGTC 2 cut(s) 169, 341
PspN4I GGNNCC 4 cut(s) 44, 86, 116, 152
PspPI GGNCC 1 cut(s) 43
PspXI VCTCGAGB 1 cut(s) 26
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
RseI CAYNNNNRTG 1 cut(s) 90
Sau3AI GATC 2 cut(s) 307, 376
Sau96I GGNCC 1 cut(s) 43
SchI GAGTC 2 cut(s) 170, 341
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 1 cut(s) 57
SetI ASST 7 cut(s) 27, 82, 131, 156, 205, 358, 428
SfcI CTRYAG 1 cut(s) 130
Sfr274I CTCGAG 1 cut(s) 26
SlaI CTCGAG 1 cut(s) 26
SmiMI CAYNNNNRTG 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 26
SmoI CTYRAG 1 cut(s) 26
Sse9I AATT 1 cut(s) 209
SspMI CTAG 2 cut(s) 167, 445
StyD4I CCNGG 1 cut(s) 87
StyI CCWWGG 1 cut(s) 237
TaqI TCGA 1 cut(s) 27
TasI AATT 1 cut(s) 209
TfiI GAWTC 1 cut(s) 99
TspDTI ATGAA 1 cut(s) 444
XapI RAATTY 1 cut(s) 209
XhoI CTCGAG 1 cut(s) 26
XspI CTAG 2 cut(s) 167, 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.