FvH4_5g37830

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001698
Physical Location & Seq
Reverse (-)
27867201 .. 27872384
5184 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g37830.t1

Sequence Viewer

Length: 1371 bp
ATGGCGTATTTCCAGAACGATGACCTCGAGTACGTCGTCAACGACTACTACGGCGACGTCGTTGAGTTTGAGGAAGACAACGACGCTTTTGCTGATAACCAGCTCCGCGGAAGCATCCGCGATTCCGACGAAGACTCGGACGACGACGACTTCGAAGACCGCCTTGATTCGAGCAAGGTGAAGACTGATACGTCGGCGTTGGAAGCCAGAAATGGGAAGGATATACAGGGAATACCGTGGGAGAGGCTCAATTTCACTAGAGATGAGTACCGCGAGGCGCGATTGAAGCAGTATAAGAACTATGAGAGCCTTGCTCAGCCTCGTCAAGACCTCGAGAAGGAGTGCTTGCAAGTGGAGAAGGGGAAGAGGTTCTATGACTTTCACTACAACACAAGGCTTGTCAAGTCAACAATCGTTCATTTTCAGCTAAGGAATTTGTTATGGGCCACATCAAAGCATGATGTATACCTGATGCAGAACTTTTCAGTAATGCATTGGAACTCTTTGCTTAAGAGAGACACCGAAGTACTCAATGTGGCTAAACCAATTGTACCTACTGAGAAACGACCTGGGTTGTTGGCTCGGCCGCTCTCTAGAGTGCAGATAAGCACTATGGCTGTCAAGGAAGATTTGTTGGTTGCTGGCGGCTTCCAAGGTGAACTAATCTGCAAGCACATTAAACAACCTGGAGTTGCTTTCTGCTCAAAACTAACAACAGATGAAAATGCCATCACTAATGCAGTGGATATTTTCCGAAACCCAACTGGATCAATGAGGGTAATGACTGCAAACAATGATGCTCAAGTTAGAGTTTTTGACATCGAAAAGTTTGCTTGCCTTAATAGCTTCTCTTTTGAGTGGTCTGTAAATAACACCTCTGTTAGTCCCGATGGTAAGCTGCTTGCAGTACTTGGTGATAGTGCAGACTGTTTGATTGCTGATGCTCAGTCCGGAAAAGCTGTCGGGGGCCTTAAAGGGCACTTAGACTATTGCTTTTCCTCAGCTTGGCACCCGGATGGACGAATATTGGCTACTGGGAACCAAGACACTACTTGTAGGCTATGGGACATAAGGAACCTCTCACAGTCCCTAGCAGTGTTGAAGGGAAGAATGGGAGCAATAAGAGCTATAAAATTTACCTCCGATGGTCGTTTTATGGCTATGGCTGAACCAGCAGACTTTGTTCACATCATTGATACCCAGTCTGGATATGCTAAGGGACAAGAGATTGACTTGTTTGGTGAGATTGCCGGAATATCCTTCAGCCCCGACTCTGAAGCTTTGTTTGTTGGGGTTGCTGATCGCACATATGGTAGTGTATTGGAGTTGAATAAGAGGCACTATAATCACTATCAAGAAATAGTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

457

Amino Acids

51.55

Weight (kDa)

5.11

Isoelectric Point (pI)

37.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 207 - 401 1.4e-18 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 246 - 359 2.1e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 253 - 428 2.5e-26 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 257 - 362 5.9e-07 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 258 - 389 1.4e-12 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 259 - 436 1.9e-20 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 259 - 403 7.8e-16 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 259 - 363 5.3e-07 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 265 - 436 2.5e-13 WDR3 first beta-propeller domain
WDR55 PF24796 283 - 408 5.9e-07 WDR55
WD40_CDC20-Fz PF24807 289 - 426 2.6e-09 CDC20/Fizzy WD40 domain
WD40 PF00400 319 - 356 2.6e-07 WD domain, G-beta repeat
WD40_Gbeta PF25391 325 - 436 5e-06 G protein beta WD-40 repeat protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 190
AatII GACGTC 1 cut(s) 60
AccB1I GGYRCC 1 cut(s) 1008
AccBSI CCGCTC 1 cut(s) 589
AccI GTMKAC 1 cut(s) 465
AccII CGCG 4 cut(s) 108, 120, 273, 280
AccIII TCCGGA 1 cut(s) 950
AciI CCGC 7 cut(s) 106, 108, 118, 160, 271, 587, 645
AclWI GGATC 1 cut(s) 775
AcoI YGGCCR 1 cut(s) 584
AcsI RAATTY 2 cut(s) 433, 1133
AcuI CTGAAG 2 cut(s) 1246, 1296
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 5 cut(s) 32, 269, 528, 552, 909
AfiI CCNNNNNNNGG 3 cut(s) 213, 337, 1005
AflII CTTAAG 1 cut(s) 509
AgsI TTSAA 3 cut(s) 286, 1102, 1330
AjnI CCWGG 2 cut(s) 568, 685
AluBI AGCT 8 cut(s) 103, 427, 846, 898, 959, 1004, 1127, 1280
AluI AGCT 8 cut(s) 103, 427, 846, 898, 959, 1004, 1127, 1280
Alw26I GTCTC 1 cut(s) 510
AlwI GGATC 1 cut(s) 775
Ama87I CYCGRG 2 cut(s) 26, 332
Aor13HI TCCGGA 1 cut(s) 950
AoxI GGCC 3 cut(s) 444, 584, 967
ApeKI GCWGC 1 cut(s) 898
ApoI RAATTY 2 cut(s) 433, 1133
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
Asp700I GAANNNNTTC 1 cut(s) 368
AspLEI GCGC 1 cut(s) 280
AspS9I GGNCC 2 cut(s) 444, 967
AsuC2I CCSGG 1 cut(s) 1013
AsuHPI GGTGA 4 cut(s) 190, 668, 926, 1253
AsuII TTCGAA 1 cut(s) 153
AvaI CYCGRG 2 cut(s) 26, 332
BaeGI GKGCMC 1 cut(s) 981
BanI GGYRCC 1 cut(s) 1008
BbsI GAAGAC 5 cut(s) 81, 138, 162, 188, 1357
BbvCI CCTCAGC 1 cut(s) 1000
BbvI GCAGC 1 cut(s) 885
BccI CCATC 4 cut(s) 737, 884, 1010, 1139
BceAI ACGGC 1 cut(s) 67
BcgI CGANNNNNNTGC 4 cut(s) 71, 105, 812, 846
BciT130I CCWGG 2 cut(s) 570, 687
BcnI CCSGG 1 cut(s) 1013
BcoDI GTCTC 1 cut(s) 510
BfaI CTAG 4 cut(s) 258, 594, 1091, 1369
BfrI CTTAAG 1 cut(s) 509
BisI GCNGC 3 cut(s) 587, 646, 899
BlpI GCTNAGC 1 cut(s) 315
BlsI GCNGC 3 cut(s) 588, 647, 900
BmcAI AGTACT 2 cut(s) 528, 909
Bme1390I CCNGG 3 cut(s) 570, 687, 1013
BmeT110I CYCGRG 2 cut(s) 26, 332
BmgT120I GGNCC 2 cut(s) 444, 967
BmiI GGNNCC 4 cut(s) 968, 1010, 1040, 1076
BmrFI CCNGG 3 cut(s) 570, 687, 1013
BmrI ACTGGG 2 cut(s) 1044, 1195
BmsI GCATC 4 cut(s) 123, 462, 787, 931
BmuI ACTGGG 2 cut(s) 1044, 1195
BpiI GAAGAC 5 cut(s) 81, 138, 162, 188, 1357
BplI GAGNNNNNCTC 2 cut(s) 298, 330
BpmI CTGGAG 1 cut(s) 708
Bpu10I CCTNAGC 3 cut(s) 428, 1000, 1215
Bpu1102I GCTNAGC 1 cut(s) 315
Bpu14I TTCGAA 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 786
BpuMI CCSGG 1 cut(s) 1013
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 4 cut(s) 106, 236, 569, 652
BsaWI WCCGGW 1 cut(s) 950
Bsc4I CCNNNNNNNGG 3 cut(s) 213, 337, 1005
Bse1I ACTGG 3 cut(s) 769, 1039, 1201
BseAI TCCGGA 1 cut(s) 950
BseBI CCWGG 2 cut(s) 570, 687
BseDI CCNNGG 4 cut(s) 106, 236, 569, 652
BseGI GGATG 2 cut(s) 114, 1021
BseLI CCNNNNNNNGG 3 cut(s) 213, 337, 1005
BseMII CTCAG 4 cut(s) 329, 549, 959, 1014
BseNI ACTGG 3 cut(s) 769, 1039, 1201
BseSI GKGCMC 1 cut(s) 981
BseX3I CGGCCG 1 cut(s) 584
BseXI GCAGC 1 cut(s) 885
BsgI GTGCAG 2 cut(s) 620, 942
Bsh1236I CGCG 4 cut(s) 108, 120, 273, 280
Bsh1285I CGRYCG 1 cut(s) 587
BshFI GGCC 3 cut(s) 446, 586, 969
BshNI GGYRCC 1 cut(s) 1008
BsiEI CGRYCG 1 cut(s) 587
BsiHKCI CYCGRG 2 cut(s) 26, 332
BsiSI CCGG 3 cut(s) 951, 1013, 1251
BslFI GGGAC 4 cut(s) 870, 1072, 1079, 1233
BslI CCNNNNNNNGG 3 cut(s) 213, 337, 1005
BsmAI GTCTC 1 cut(s) 510
BsmFI GGGAC 4 cut(s) 870, 1072, 1079, 1233
BsnI GGCC 3 cut(s) 446, 586, 969
BsoBI CYCGRG 2 cut(s) 26, 332
Bsp119I TTCGAA 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 981
Bsp13I TCCGGA 1 cut(s) 950
Bsp143I GATC 2 cut(s) 767, 1300
Bsp1720I GCTNAGC 1 cut(s) 315
BspACI CCGC 7 cut(s) 106, 108, 118, 160, 271, 587, 645
BspANI GGCC 3 cut(s) 446, 586, 969
BspCNI CTCAG 4 cut(s) 328, 550, 958, 1013
BspEI TCCGGA 1 cut(s) 950
BspFNI CGCG 4 cut(s) 108, 120, 273, 280
BspLI GGNNCC 4 cut(s) 968, 1010, 1040, 1076
BspPI GGATC 1 cut(s) 775
BspT104I TTCGAA 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 1008
BspTI CTTAAG 1 cut(s) 509
BsrBI CCGCTC 1 cut(s) 589
BsrI ACTGG 3 cut(s) 769, 1039, 1201
BssECI CCNNGG 4 cut(s) 106, 236, 569, 652
BssMI GATC 2 cut(s) 767, 1300
BssNAI GTATAC 1 cut(s) 466
BssNI GRCGYC 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 652
Bst1107I GTATAC 1 cut(s) 466
Bst2UI CCWGG 2 cut(s) 570, 687
Bst4CI ACNGT 3 cut(s) 237, 929, 1086
Bst6I CTCTTC 1 cut(s) 359
BstACI GRCGYC 1 cut(s) 57
BstAFI CTTAAG 1 cut(s) 509
BstBI TTCGAA 1 cut(s) 153
BstC8I GCNNGC 5 cut(s) 347, 643, 671, 835, 903
BstDEI CTNAG 7 cut(s) 315, 428, 558, 945, 982, 1000, 1215
BstDSI CCRYGG 2 cut(s) 106, 236
BstENI CCTNNNNNAGG 1 cut(s) 335
BstF5I GGATG 2 cut(s) 114, 1021
BstFNI CGCG 4 cut(s) 108, 120, 273, 280
BstHHI GCGC 1 cut(s) 280
BstKTI GATC 2 cut(s) 770, 1303
BstMAI GTCTC 1 cut(s) 510
BstMBI GATC 2 cut(s) 767, 1300
BstMCI CGRYCG 1 cut(s) 587
BstMWI GCNNNNNNNGC 5 cut(s) 203, 286, 843, 1124, 1172
BstNI CCWGG 2 cut(s) 570, 687
BstSCI CCNGG 3 cut(s) 568, 685, 1011
BstSLI GKGCMC 1 cut(s) 981
BstUI CGCG 4 cut(s) 108, 120, 273, 280
BstV1I GCAGC 1 cut(s) 885
BstV2I GAAGAC 5 cut(s) 81, 138, 162, 188, 1357
BstZ17I GTATAC 1 cut(s) 466
BstZI CGGCCG 1 cut(s) 584
BsuRI GGCC 3 cut(s) 446, 586, 969
BtgI CCRYGG 2 cut(s) 106, 236
BtsCI GGATG 2 cut(s) 114, 1021
BtsI GCAGTG 2 cut(s) 747, 1101
BtsIMutI CAGTG 2 cut(s) 747, 1101
Cac8I GCNNGC 5 cut(s) 347, 643, 671, 835, 903
CfoI GCGC 1 cut(s) 280
Cfr13I GGNCC 2 cut(s) 444, 967
Cfr42I CCGCGG 1 cut(s) 109
CseI GACGC 1 cut(s) 92
Csp6I GTAC 5 cut(s) 31, 268, 527, 551, 908
CviAII CATG 1 cut(s) 458
CviQI GTAC 5 cut(s) 31, 268, 527, 551, 908
DdeI CTNAG 7 cut(s) 315, 428, 558, 945, 982, 1000, 1215
DpnI GATC 2 cut(s) 769, 1302
DpnII GATC 2 cut(s) 767, 1300
DrdI GACNNNNNNGTC 1 cut(s) 190
DseDI GACNNNNNNGTC 1 cut(s) 190
EaeI YGGCCR 1 cut(s) 584
EagI CGGCCG 1 cut(s) 584
Eam1104I CTCTTC 1 cut(s) 359
EarI CTCTTC 1 cut(s) 359
EclXI CGGCCG 1 cut(s) 584
Eco130I CCWWGG 1 cut(s) 652
Eco52I CGGCCG 1 cut(s) 584
Eco57I CTGAAG 2 cut(s) 1246, 1296
Eco88I CYCGRG 2 cut(s) 26, 332
EcoNI CCTNNNNNAGG 1 cut(s) 335
EcoO109I RGGNCCY 1 cut(s) 967
EcoRII CCWGG 2 cut(s) 568, 685
EcoT14I CCWWGG 1 cut(s) 652
EcoT22I ATGCAT 1 cut(s) 495
ErhI CCWWGG 1 cut(s) 652
FaeI CATG 1 cut(s) 461
FalI AAGNNNNNCTT 4 cut(s) 147, 179, 329, 361
FaqI GGGAC 4 cut(s) 870, 1072, 1079, 1233
FatI CATG 1 cut(s) 457
FauNDI CATATG 1 cut(s) 1309
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 3 cut(s) 587, 646, 899
FokI GGATG 2 cut(s) 101, 1028
Fsp4HI GCNGC 3 cut(s) 587, 646, 899
FspBI CTAG 4 cut(s) 258, 594, 1091, 1369
GlaI GCGC 1 cut(s) 279
GluI GCNGC 3 cut(s) 587, 646, 899
GsuI CTGGAG 1 cut(s) 708
HaeIII GGCC 3 cut(s) 446, 586, 969
HapII CCGG 3 cut(s) 951, 1013, 1251
HgaI GACGC 1 cut(s) 92
HhaI GCGC 1 cut(s) 280
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 1 cut(s) 461
Hin6I GCGC 1 cut(s) 278
HinP1I GCGC 1 cut(s) 278
HincII GTYRAC 2 cut(s) 40, 408
HindII GTYRAC 2 cut(s) 40, 408
HindIII AAGCTT 1 cut(s) 1278
HinfI GANTC 4 cut(s) 122, 134, 167, 1271
HpaII CCGG 3 cut(s) 951, 1013, 1251
HphI GGTGA 4 cut(s) 190, 668, 926, 1253
Hpy166II GTNNAC 5 cut(s) 40, 408, 466, 659, 1186
Hpy188I TCNGA 5 cut(s) 127, 139, 755, 1144, 1276
Hpy188III TCNNGA 8 cut(s) 13, 326, 334, 594, 887, 951, 1206, 1355
Hpy8I GTNNAC 5 cut(s) 40, 408, 466, 659, 1186
Hpy99I CGWCG 8 cut(s) 38, 59, 62, 86, 131, 146, 149, 196
HpyAV CCTTC 5 cut(s) 211, 331, 352, 1096, 1270
HpyCH4III ACNGT 3 cut(s) 237, 929, 1086
HpyCH4IV ACGT 3 cut(s) 33, 57, 191
HpyCH4V TGCA 9 cut(s) 349, 475, 493, 601, 669, 740, 788, 905, 923
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 286, 843, 1124, 1172
HpyF3I CTNAG 7 cut(s) 315, 428, 558, 945, 982, 1000, 1215
HpySE526I ACGT 3 cut(s) 33, 57, 191
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 1 cut(s) 461
HspAI GCGC 1 cut(s) 278
Kpn2I TCCGGA 1 cut(s) 950
KspI CCGCGG 1 cut(s) 109
Kzo9I GATC 2 cut(s) 767, 1300
LmnI GCTCC 2 cut(s) 108, 1115
Lsp1109I GCAGC 1 cut(s) 885
LweI GCATC 4 cut(s) 123, 462, 787, 931
MaeI CTAG 4 cut(s) 258, 594, 1091, 1369
MaeII ACGT 3 cut(s) 33, 57, 191
MalI GATC 2 cut(s) 769, 1302
MbiI CCGCTC 1 cut(s) 589
MboI GATC 2 cut(s) 767, 1300
MboII GAAGA 8 cut(s) 86, 143, 167, 193, 376, 638, 1119, 1357
MfeI CAATTG 1 cut(s) 546
MhlI GDGCHC 1 cut(s) 981
MluCI AATT 4 cut(s) 250, 433, 546, 1133
MlyI GAGTC 2 cut(s) 128, 1265
MmeI TCCRAC 2 cut(s) 150, 180
Mph1103I ATGCAT 1 cut(s) 495
MroI TCCGGA 1 cut(s) 950
MroXI GAANNNNTTC 1 cut(s) 368
MseI TTAA 4 cut(s) 510, 678, 840, 972
MslI CAYNNNNRTG 1 cut(s) 1014
MspA1I CMGCKG 1 cut(s) 108
MspCI CTTAAG 1 cut(s) 509
MspI CCGG 3 cut(s) 951, 1013, 1251
MspR9I CCNGG 3 cut(s) 570, 687, 1013
MunI CAATTG 1 cut(s) 546
MvaI CCWGG 2 cut(s) 570, 687
MvnI CGCG 4 cut(s) 108, 120, 273, 280
MwoI GCNNNNNNNGC 5 cut(s) 203, 286, 843, 1124, 1172
NciI CCSGG 1 cut(s) 1013
NdeI CATATG 1 cut(s) 1309
NdeII GATC 2 cut(s) 767, 1300
NlaIII CATG 1 cut(s) 461
NlaIV GGNNCC 4 cut(s) 968, 1010, 1040, 1076
NmeAIII GCCGAG 1 cut(s) 562
NsiI ATGCAT 1 cut(s) 495
NspV TTCGAA 1 cut(s) 153
PaeR7I CTCGAG 2 cut(s) 26, 332
PcsI WCGNNNNNNNCGW 5 cut(s) 24, 33, 39, 57, 150
PdmI GAANNNNTTC 1 cut(s) 368
PfeI GAWTC 2 cut(s) 122, 167
PkrI GCNGC 3 cut(s) 588, 647, 900
PleI GAGTC 2 cut(s) 128, 1265
PpsI GAGTC 2 cut(s) 128, 1265
Psp6I CCWGG 2 cut(s) 568, 685
PspGI CCWGG 2 cut(s) 568, 685
PspN4I GGNNCC 4 cut(s) 968, 1010, 1040, 1076
PspPI GGNCC 2 cut(s) 444, 967
PspXI VCTCGAGB 1 cut(s) 26
RsaI GTAC 5 cut(s) 32, 269, 528, 552, 909
RsaNI GTAC 5 cut(s) 31, 268, 527, 551, 908
RseI CAYNNNNRTG 1 cut(s) 1014
SacII CCGCGG 1 cut(s) 109
SaqAI TTAA 4 cut(s) 510, 678, 840, 972
SatI GCNGC 3 cut(s) 587, 646, 899
Sau3AI GATC 2 cut(s) 767, 1300
Sau96I GGNCC 2 cut(s) 444, 967
ScaI AGTACT 2 cut(s) 528, 909
SchI GAGTC 2 cut(s) 128, 1265
ScrFI CCNGG 3 cut(s) 570, 687, 1013
SduI GDGCHC 1 cut(s) 981
SfaNI GCATC 4 cut(s) 123, 462, 787, 931
Sfr274I CTCGAG 2 cut(s) 26, 332
Sfr303I CCGCGG 1 cut(s) 109
SfuI TTCGAA 1 cut(s) 153
SgrBI CCGCGG 1 cut(s) 109
SlaI CTCGAG 2 cut(s) 26, 332
SmiMI CAYNNNNRTG 1 cut(s) 1014
SmlI CTYRAG 4 cut(s) 26, 332, 509, 801
SmoI CTYRAG 4 cut(s) 26, 332, 509, 801
Sse9I AATT 4 cut(s) 250, 433, 546, 1133
SsiI CCGC 7 cut(s) 106, 108, 118, 160, 271, 587, 645
SspI AATATT 1 cut(s) 1026
SspMI CTAG 4 cut(s) 258, 594, 1091, 1369
StyD4I CCNGG 3 cut(s) 568, 685, 1011
StyI CCWWGG 1 cut(s) 652
TaaI ACNGT 3 cut(s) 237, 929, 1086
TaiI ACGT 3 cut(s) 36, 60, 194
TaqI TCGA 5 cut(s) 27, 153, 170, 333, 822
TasI AATT 4 cut(s) 250, 433, 546, 1133
TatI WGTACW 2 cut(s) 526, 907
TauI GCSGC 2 cut(s) 589, 648
TfiI GAWTC 2 cut(s) 122, 167
Tru1I TTAA 4 cut(s) 510, 678, 840, 972
Tru9I TTAA 4 cut(s) 510, 678, 840, 972
TscAI CASTG 2 cut(s) 747, 1101
TseI GCWGC 1 cut(s) 898
TspDTI ATGAA 2 cut(s) 407, 735
TspRI CASTG 2 cut(s) 747, 1101
Vha464I CTTAAG 1 cut(s) 509
XagI CCTNNNNNAGG 1 cut(s) 335
XapI RAATTY 2 cut(s) 433, 1133
XbaI TCTAGA 1 cut(s) 593
XhoI CTCGAG 2 cut(s) 26, 332
XmiI GTMKAC 1 cut(s) 465
XmnI GAANNNNTTC 1 cut(s) 368
XspI CTAG 4 cut(s) 258, 594, 1091, 1369
ZraI GACGTC 1 cut(s) 58
ZrmI AGTACT 2 cut(s) 528, 909
Zsp2I ATGCAT 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.