RchiOBHm_Chr7g0239731

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001698
Physical Location & Seq
Reverse (-)
65510169 .. 65511181
1013 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21486

Sequence Viewer

Length: 474 bp
ATGGCTGTCAAGGAAAATTTGTTGGTTGCTGGGGGCTTCCTAGGTGAACTAATCTGCAAGCACATGAAACAACCTGGAGTTGCTTTCTGCTCAAAACTAACAACAGATGAGAATGCCATCACTAATGCAGTGGATATTTTTCAAAACCCAACTGGCTCAATGAGGGTAATGACTGCAAATGATGATGCTCAAGTTAGAGTTTTTGAAATTGAAAAGTTTGCTTGCCTAAATCGCTTCTCCTTCGGGTGGTCTGTAAATAACACCTCTGTTAGTCCTGATGGTAAGCTGCTCGCAGTACTTGGTGATAGTGCAGACTGTTTGATTGCTGAGACTCAGTCTGGTAAAGCTGTTGGGGGCCTCAAAGGGCACTTAGACTATTGCTTTTCTTCAGATTGGCACCCGGATGGACGAATCTTGGCTCCTGGGAACCAAGACACTACCTGTAGGCTATGGGACATAAGGAACCTGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

157

Amino Acids

17.03

Weight (kDa)

5.27

Isoelectric Point (pI)

27.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 5 - 155 2e-11 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 31 - 156 1.7e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 41 - 155 1.3e-08 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 45 - 156 1.1e-14 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 52 - 155 1.2e-12 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 53 - 154 2.5e-09 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 53 - 155 7.2e-07 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 55 - 154 9.2e-10 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 396
AcsI RAATTY 1 cut(s) 16
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 1 cut(s) 297
AfiI CCNNNNNNNGG 1 cut(s) 246
AgsI TTSAA 3 cut(s) 143, 206, 212
AjnI CCWGG 2 cut(s) 73, 421
AluBI AGCT 2 cut(s) 286, 347
AluI AGCT 2 cut(s) 286, 347
Alw26I GTCTC 1 cut(s) 323
AoxI GGCC 1 cut(s) 355
ApeKI GCWGC 1 cut(s) 286
ApoI RAATTY 1 cut(s) 16
AspA2I CCTAGG 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 355
AsuC2I CCSGG 1 cut(s) 401
AsuHPI GGTGA 2 cut(s) 56, 314
AvrII CCTAGG 1 cut(s) 40
BaeGI GKGCMC 1 cut(s) 369
BanI GGYRCC 1 cut(s) 396
BbvI GCAGC 1 cut(s) 273
BccI CCATC 3 cut(s) 125, 272, 398
BciT130I CCWGG 2 cut(s) 75, 423
BcnI CCSGG 1 cut(s) 401
BcoDI GTCTC 1 cut(s) 323
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 442
BisI GCNGC 1 cut(s) 287
BlnI CCTAGG 1 cut(s) 40
BlsI GCNGC 1 cut(s) 288
BmcAI AGTACT 1 cut(s) 297
Bme1390I CCNGG 3 cut(s) 75, 401, 423
BmgT120I GGNCC 1 cut(s) 355
BmiI GGNNCC 5 cut(s) 356, 398, 420, 428, 464
BmrFI CCNGG 3 cut(s) 75, 401, 423
BmsI GCATC 1 cut(s) 175
BpmI CTGGAG 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 174
BpuMI CCSGG 1 cut(s) 401
BsaJI CCNNGG 2 cut(s) 40, 422
Bsc4I CCNNNNNNNGG 1 cut(s) 246
Bse1I ACTGG 1 cut(s) 157
BseBI CCWGG 2 cut(s) 75, 423
BseDI CCNNGG 2 cut(s) 40, 422
BseGI GGATG 1 cut(s) 409
BseLI CCNNNNNNNGG 1 cut(s) 246
BseMII CTCAG 2 cut(s) 318, 347
BseNI ACTGG 1 cut(s) 157
BseSI GKGCMC 1 cut(s) 369
BseXI GCAGC 1 cut(s) 273
BseYI CCCAGC 1 cut(s) 29
BsgI GTGCAG 1 cut(s) 330
BshFI GGCC 1 cut(s) 357
BshNI GGYRCC 1 cut(s) 396
BsiSI CCGG 1 cut(s) 401
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 1 cut(s) 246
BsmAI GTCTC 1 cut(s) 323
BsmFI GGGAC 1 cut(s) 467
BsmI GAATGC 1 cut(s) 118
BsnI GGCC 1 cut(s) 357
Bsp1286I GDGCHC 1 cut(s) 369
BspANI GGCC 1 cut(s) 357
BspCNI CTCAG 2 cut(s) 319, 346
BspLI GGNNCC 5 cut(s) 356, 398, 420, 428, 464
BspT107I GGYRCC 1 cut(s) 396
BsrI ACTGG 1 cut(s) 157
BssECI CCNNGG 2 cut(s) 40, 422
BssT1I CCWWGG 1 cut(s) 40
Bst2UI CCWGG 2 cut(s) 75, 423
Bst4CI ACNGT 1 cut(s) 317
BstC8I GCNNGC 3 cut(s) 59, 223, 291
BstDEI CTNAG 3 cut(s) 327, 333, 370
BstF5I GGATG 1 cut(s) 409
BstMAI GTCTC 1 cut(s) 323
BstMWI GCNNNNNNNGC 1 cut(s) 231
BstNI CCWGG 2 cut(s) 75, 423
BstSCI CCNGG 3 cut(s) 73, 399, 421
BstSFI CTRYAG 1 cut(s) 442
BstSLI GKGCMC 1 cut(s) 369
BstV1I GCAGC 1 cut(s) 273
BsuRI GGCC 1 cut(s) 357
BtsCI GGATG 1 cut(s) 409
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 3 cut(s) 59, 223, 291
Cfr13I GGNCC 1 cut(s) 355
Csp6I GTAC 1 cut(s) 296
CviAII CATG 1 cut(s) 64
CviJI RGCY 8 cut(s) 5, 36, 156, 286, 347, 357, 419, 448
CviKI_1 RGCY 8 cut(s) 5, 36, 156, 286, 347, 357, 419, 448
CviQI GTAC 1 cut(s) 296
DdeI CTNAG 3 cut(s) 327, 333, 370
Eco130I CCWWGG 1 cut(s) 40
Eco57I CTGAAG 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 2 cut(s) 73, 421
EcoT14I CCWWGG 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 1 cut(s) 67
FaiI YATR 4 cut(s) 65, 451, 458, 472
FaqI GGGAC 1 cut(s) 467
FatI CATG 1 cut(s) 63
Fnu4HI GCNGC 1 cut(s) 287
FokI GGATG 1 cut(s) 416
Fsp4HI GCNGC 1 cut(s) 287
FspBI CTAG 1 cut(s) 41
GluI GCNGC 1 cut(s) 287
GsaI CCCAGC 1 cut(s) 33
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 1 cut(s) 357
HapII CCGG 1 cut(s) 401
Hin1II CATG 1 cut(s) 67
HinfI GANTC 2 cut(s) 331, 411
HpaII CCGG 1 cut(s) 401
HphI GGTGA 2 cut(s) 56, 314
Hpy166II GTNNAC 1 cut(s) 47
Hpy188I TCNGA 1 cut(s) 391
Hpy188III TCNNGA 1 cut(s) 275
Hpy8I GTNNAC 1 cut(s) 47
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4V TGCA 4 cut(s) 57, 128, 176, 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 231
HpyF3I CTNAG 3 cut(s) 327, 333, 370
Hsp92II CATG 1 cut(s) 67
LmnI GCTCC 1 cut(s) 424
Lsp1109I GCAGC 1 cut(s) 273
LweI GCATC 1 cut(s) 175
MaeI CTAG 1 cut(s) 41
MboII GAAGA 1 cut(s) 378
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 2 cut(s) 16, 207
MlyI GAGTC 1 cut(s) 325
MnlI CCTC 3 cut(s) 156, 274, 368
MslI CAYNNNNRTG 1 cut(s) 402
MspI CCGG 1 cut(s) 401
MspR9I CCNGG 3 cut(s) 75, 401, 423
Mva1269I GAATGC 1 cut(s) 118
MvaI CCWGG 2 cut(s) 75, 423
MwoI GCNNNNNNNGC 1 cut(s) 231
NciI CCSGG 1 cut(s) 401
NlaIII CATG 1 cut(s) 67
NlaIV GGNNCC 5 cut(s) 356, 398, 420, 428, 464
PctI GAATGC 1 cut(s) 118
PfeI GAWTC 1 cut(s) 411
PflFI GACNNNGTC 1 cut(s) 334
PkrI GCNGC 1 cut(s) 288
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
Psp6I CCWGG 2 cut(s) 73, 421
PspFI CCCAGC 1 cut(s) 29
PspGI CCWGG 2 cut(s) 73, 421
PspN4I GGNNCC 5 cut(s) 356, 398, 420, 428, 464
PspPI GGNCC 1 cut(s) 355
PsyI GACNNNGTC 1 cut(s) 334
RsaI GTAC 1 cut(s) 297
RsaNI GTAC 1 cut(s) 296
RseI CAYNNNNRTG 1 cut(s) 402
SatI GCNGC 1 cut(s) 287
Sau96I GGNCC 1 cut(s) 355
ScaI AGTACT 1 cut(s) 297
SchI GAGTC 1 cut(s) 325
ScrFI CCNGG 3 cut(s) 75, 401, 423
SduI GDGCHC 1 cut(s) 369
SetI ASST 7 cut(s) 46, 76, 266, 288, 349, 443, 468
SfaNI GCATC 1 cut(s) 175
SfcI CTRYAG 1 cut(s) 442
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 1 cut(s) 189
SmoI CTYRAG 1 cut(s) 189
Sse9I AATT 2 cut(s) 16, 207
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 3 cut(s) 73, 399, 421
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 1 cut(s) 317
TasI AATT 2 cut(s) 16, 207
TatI WGTACW 1 cut(s) 295
TfiI GAWTC 1 cut(s) 411
TscAI CASTG 1 cut(s) 135
TseI GCWGC 1 cut(s) 286
TspDTI ATGAA 1 cut(s) 80
TspRI CASTG 1 cut(s) 135
Tth111I GACNNNGTC 1 cut(s) 334
XapI RAATTY 1 cut(s) 16
XmaJI CCTAGG 1 cut(s) 40
XspI CTAG 1 cut(s) 41
ZrmI AGTACT 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.