Rw7G040520

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001698
Physical Location & Seq
Reverse (-)
63083308 .. 63086045
2738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G040520.1

Sequence Viewer

Length: 903 bp
ATGTACAACACAAGGCTTGTCAAGTCCACAATTGTGCATTTTCAGCTAAGGAATTTGTTATGGGCCACATCAAAGCATGATGTATACCTGATGCAGAACTTCTCAGTGATGCATTGGAACTCCTTGCACAGGAGAGGCAGCGAAGTACTCAATGTGGCCAAACCAATTGTACCTACTGAGATGAGCACTATGGTTCTCAAGGACAATTTGTTGGTTGCTGTGGGCTTCCAAGGTGAACAAATCTGCAAGCACATGAAACAACCTGGAGTTGCTTTCTGCTCAAAACTAACGACAGATGAGAATGCCATCACTAATGCAGTGGATATTTTCCAAAACCCACCGGTAATGACTGCAAATAATGATGCTCGAGTTAGAGTTTTTTACGTTGAAAAGTTCGTTTGCGTCAACCGCTTCTCCTTGGAGTGGTCTGTAAATAACACTTCTGTTAGTCCCGATGGTAAGCTGCTCGCAGTACTTGGTGATAGTGCAGACTGTTTGATAGCTGATGCTCAGTCTGGAAAAGCTGTCGGGGGCCTGAAAGGGCACTTAGACTATTGCTTTTCCTCAGCTTGGCACCCGGATGCGCGAATCTTAGCTACTGGGAACCAAGACACTACTTGCAGGTTATGGGACGTAAGGAACCTGTCAGAGTCCCTAGGCTATAAAATTCAATTTGATGGTCGTTTTATGGCTATGGCTGAGCCAGCAGACTTTGTTCACATCATTGATACCCAGTCCGGATATGCAAAGGAACAAGAGATTGATCTGTTTGGTGAGATTGCCGGAATATCCTTCAGCCCCGACTCTGAAGCTTTGTTTATTGGGGTTGCTGATCGCACATATGGTAGTGTATTGGAGTTCAATAAGAGGCACTATAATCCCTATCAAGAAATAGTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

300

Amino Acids

33.63

Weight (kDa)

5.84

Isoelectric Point (pI)

36.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 69 - 221 4.6e-08 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 92 - 214 9e-11 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 95 - 214 6.8e-09 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 112 - 215 1.4e-09 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 112 - 213 1e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 115 - 199 1.8e-11 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 115 - 214 2.5e-12 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 115 - 218 3.4e-06 WDR3 second beta-propeller domain
WD40 PF00400 174 - 211 1.7e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 180 - 275 6.2e-09 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 612
AccB1I GGYRCC 1 cut(s) 573
AccI GTMKAC 1 cut(s) 84
AccII CGCG 1 cut(s) 586
AccIII TCCGGA 1 cut(s) 737
AciI CCGC 1 cut(s) 409
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 2 cut(s) 52, 666
AcuI CTGAAG 2 cut(s) 778, 828
AfaI GTAC 4 cut(s) 5, 147, 171, 474
AfiI CCNNNNNNNGG 3 cut(s) 129, 423, 570
AgeI ACCGGT 1 cut(s) 340
AgsI TTSAA 3 cut(s) 389, 671, 862
AjnI CCWGG 1 cut(s) 262
AleI CACNNNNGTG 1 cut(s) 32
AluBI AGCT 7 cut(s) 46, 463, 503, 524, 569, 596, 812
AluI AGCT 7 cut(s) 46, 463, 503, 524, 569, 596, 812
Alw21I GWGCWC 1 cut(s) 188
Ama87I CYCGRG 1 cut(s) 366
Aor13HI TCCGGA 1 cut(s) 737
AoxI GGCC 3 cut(s) 63, 156, 532
ApeKI GCWGC 2 cut(s) 138, 463
ApoI RAATTY 2 cut(s) 52, 666
AsiGI ACCGGT 1 cut(s) 340
AspA2I CCTAGG 1 cut(s) 655
AspLEI GCGC 1 cut(s) 586
AspS9I GGNCC 2 cut(s) 63, 532
AsuC2I CCSGG 1 cut(s) 578
AsuHPI GGTGA 3 cut(s) 245, 491, 785
AvaI CYCGRG 1 cut(s) 366
AvrII CCTAGG 1 cut(s) 655
BaeGI GKGCMC 1 cut(s) 546
BalI TGGCCA 1 cut(s) 158
BanI GGYRCC 1 cut(s) 573
BbsI GAAGAC 1 cut(s) 889
Bbv12I GWGCWC 1 cut(s) 188
BbvCI CCTCAGC 1 cut(s) 565
BbvI GCAGC 2 cut(s) 150, 450
BccI CCATC 3 cut(s) 314, 449, 671
BciT130I CCWGG 1 cut(s) 264
BcnI CCSGG 1 cut(s) 578
BfaI CTAG 2 cut(s) 656, 901
BfuAI ACCTGC 1 cut(s) 612
BisI GCNGC 2 cut(s) 139, 464
BlnI CCTAGG 1 cut(s) 655
BlpI GCTNAGC 1 cut(s) 699
BlsI GCNGC 2 cut(s) 140, 465
BmcAI AGTACT 2 cut(s) 147, 474
Bme1390I CCNGG 2 cut(s) 264, 578
BmeT110I CYCGRG 1 cut(s) 366
BmgT120I GGNCC 2 cut(s) 63, 532
BmiI GGNNCC 4 cut(s) 533, 575, 605, 641
BmrFI CCNGG 2 cut(s) 264, 578
BmrI ACTGGG 2 cut(s) 609, 727
BmsI GCATC 5 cut(s) 81, 99, 352, 496, 571
BmuI ACTGGG 2 cut(s) 609, 727
BpiI GAAGAC 1 cut(s) 889
BpmI CTGGAG 1 cut(s) 285
Bpu10I CCTNAGC 2 cut(s) 47, 565
Bpu1102I GCTNAGC 1 cut(s) 699
BpuEI CTTGAG 1 cut(s) 182
BpuMI CCSGG 1 cut(s) 578
BsaJI CCNNGG 3 cut(s) 229, 417, 655
BsaWI WCCGGW 2 cut(s) 340, 737
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 423, 570
Bse118I RCCGGY 1 cut(s) 340
Bse1I ACTGG 2 cut(s) 604, 733
BseAI TCCGGA 1 cut(s) 737
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 3 cut(s) 229, 417, 655
BseGI GGATG 1 cut(s) 586
BseLI CCNNNNNNNGG 3 cut(s) 129, 423, 570
BseMII CTCAG 5 cut(s) 117, 168, 524, 579, 690
BseNI ACTGG 2 cut(s) 604, 733
BseSI GKGCMC 1 cut(s) 546
BseXI GCAGC 2 cut(s) 150, 450
BsgI GTGCAG 1 cut(s) 507
Bsh1236I CGCG 1 cut(s) 586
BshFI GGCC 3 cut(s) 65, 158, 534
BshNI GGYRCC 1 cut(s) 573
BshTI ACCGGT 1 cut(s) 340
BsiHKAI GWGCWC 1 cut(s) 188
BsiHKCI CYCGRG 1 cut(s) 366
BsiSI CCGG 4 cut(s) 341, 578, 738, 783
BslFI GGGAC 3 cut(s) 435, 637, 644
BslI CCNNNNNNNGG 3 cut(s) 129, 423, 570
BsmFI GGGAC 3 cut(s) 435, 637, 644
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 3 cut(s) 65, 158, 534
BsoBI CYCGRG 1 cut(s) 366
Bsp1286I GDGCHC 2 cut(s) 188, 546
Bsp13I TCCGGA 1 cut(s) 737
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 763, 832
Bsp1720I GCTNAGC 1 cut(s) 699
BspACI CCGC 1 cut(s) 409
BspANI GGCC 3 cut(s) 65, 158, 534
BspCNI CTCAG 5 cut(s) 116, 169, 523, 578, 691
BspEI TCCGGA 1 cut(s) 737
BspFNI CGCG 1 cut(s) 586
BspLI GGNNCC 4 cut(s) 533, 575, 605, 641
BspMI ACCTGC 1 cut(s) 612
BspT107I GGYRCC 1 cut(s) 573
BsrFI RCCGGY 1 cut(s) 340
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 604, 733
BssAI RCCGGY 1 cut(s) 340
BssECI CCNNGG 3 cut(s) 229, 417, 655
BssMI GATC 2 cut(s) 763, 832
BssNAI GTATAC 1 cut(s) 85
BssT1I CCWWGG 3 cut(s) 229, 417, 655
Bst1107I GTATAC 1 cut(s) 85
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 1 cut(s) 494
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 3 cut(s) 248, 468, 705
BstDEI CTNAG 8 cut(s) 47, 103, 177, 510, 547, 565, 592, 699
BstENI CCTNNNNNAGG 1 cut(s) 127
BstF5I GGATG 1 cut(s) 586
BstFNI CGCG 1 cut(s) 586
BstHHI GCGC 1 cut(s) 586
BstKTI GATC 2 cut(s) 766, 835
BstMBI GATC 2 cut(s) 763, 832
BstMWI GCNNNNNNNGC 3 cut(s) 43, 408, 704
BstNI CCWGG 1 cut(s) 264
BstSCI CCNGG 2 cut(s) 262, 576
BstSLI GKGCMC 1 cut(s) 546
BstUI CGCG 1 cut(s) 586
BstV1I GCAGC 2 cut(s) 150, 450
BstV2I GAAGAC 1 cut(s) 889
BstZ17I GTATAC 1 cut(s) 85
BsuRI GGCC 3 cut(s) 65, 158, 534
BtsCI GGATG 1 cut(s) 586
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 2 cut(s) 111, 324
BveI ACCTGC 1 cut(s) 612
Cac8I GCNNGC 3 cut(s) 248, 468, 705
CfoI GCGC 1 cut(s) 586
Cfr10I RCCGGY 1 cut(s) 340
Cfr13I GGNCC 2 cut(s) 63, 532
CseI GACGC 1 cut(s) 391
Csp6I GTAC 4 cut(s) 4, 146, 170, 473
CspAI ACCGGT 1 cut(s) 340
CviAII CATG 2 cut(s) 77, 253
CviQI GTAC 4 cut(s) 4, 146, 170, 473
DdeI CTNAG 8 cut(s) 47, 103, 177, 510, 547, 565, 592, 699
DpnI GATC 2 cut(s) 765, 834
DpnII GATC 2 cut(s) 763, 832
EaeI YGGCCR 1 cut(s) 156
Eco130I CCWWGG 3 cut(s) 229, 417, 655
Eco57I CTGAAG 2 cut(s) 778, 828
Eco88I CYCGRG 1 cut(s) 366
EcoNI CCTNNNNNAGG 1 cut(s) 127
EcoO109I RGGNCCY 1 cut(s) 532
EcoRII CCWGG 1 cut(s) 262
EcoT14I CCWWGG 3 cut(s) 229, 417, 655
EcoT22I ATGCAT 1 cut(s) 114
ErhI CCWWGG 3 cut(s) 229, 417, 655
FaeI CATG 2 cut(s) 80, 256
FaqI GGGAC 3 cut(s) 435, 637, 644
FatI CATG 2 cut(s) 76, 252
FauNDI CATATG 1 cut(s) 841
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 2 cut(s) 139, 464
FokI GGATG 1 cut(s) 593
Fsp4HI GCNGC 2 cut(s) 139, 464
FspBI CTAG 2 cut(s) 656, 901
GlaI GCGC 1 cut(s) 585
GluI GCNGC 2 cut(s) 139, 464
GsuI CTGGAG 1 cut(s) 285
HaeIII GGCC 3 cut(s) 65, 158, 534
HapII CCGG 4 cut(s) 341, 578, 738, 783
HgaI GACGC 1 cut(s) 391
HhaI GCGC 1 cut(s) 586
Hin1II CATG 2 cut(s) 80, 256
Hin6I GCGC 1 cut(s) 584
HinP1I GCGC 1 cut(s) 584
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HindIII AAGCTT 1 cut(s) 810
HinfI GANTC 3 cut(s) 588, 650, 803
HpaII CCGG 4 cut(s) 341, 578, 738, 783
HphI GGTGA 3 cut(s) 245, 491, 785
Hpy166II GTNNAC 5 cut(s) 27, 85, 236, 406, 718
Hpy188I TCNGA 2 cut(s) 649, 808
Hpy188III TCNNGA 4 cut(s) 452, 516, 738, 887
Hpy8I GTNNAC 5 cut(s) 27, 85, 236, 406, 718
HpyAV CCTTC 1 cut(s) 802
HpyCH4III ACNGT 1 cut(s) 494
HpyCH4IV ACGT 2 cut(s) 384, 633
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 408, 704
HpyF3I CTNAG 8 cut(s) 47, 103, 177, 510, 547, 565, 592, 699
HpySE526I ACGT 2 cut(s) 384, 633
Hsp92II CATG 2 cut(s) 80, 256
HspAI GCGC 1 cut(s) 584
Kpn2I TCCGGA 1 cut(s) 737
Kzo9I GATC 2 cut(s) 763, 832
Lsp1109I GCAGC 2 cut(s) 150, 450
LweI GCATC 5 cut(s) 81, 99, 352, 496, 571
MaeI CTAG 2 cut(s) 656, 901
MaeII ACGT 2 cut(s) 384, 633
MalI GATC 2 cut(s) 765, 834
MboI GATC 2 cut(s) 763, 832
MboII GAAGA 1 cut(s) 889
MfeI CAATTG 2 cut(s) 30, 165
MhlI GDGCHC 2 cut(s) 188, 546
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 6 cut(s) 30, 52, 165, 205, 666, 671
MluNI TGGCCA 1 cut(s) 158
MlyI GAGTC 2 cut(s) 659, 797
MnlI CCTC 3 cut(s) 128, 574, 861
Mox20I TGGCCA 1 cut(s) 158
Mph1103I ATGCAT 1 cut(s) 114
MroI TCCGGA 1 cut(s) 737
MscI TGGCCA 1 cut(s) 158
MslI CAYNNNNRTG 2 cut(s) 32, 579
Msp20I TGGCCA 1 cut(s) 158
MspI CCGG 4 cut(s) 341, 578, 738, 783
MspR9I CCNGG 2 cut(s) 264, 578
MunI CAATTG 2 cut(s) 30, 165
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 1 cut(s) 264
MvnI CGCG 1 cut(s) 586
MwoI GCNNNNNNNGC 3 cut(s) 43, 408, 704
NciI CCSGG 1 cut(s) 578
NdeI CATATG 1 cut(s) 841
NdeII GATC 2 cut(s) 763, 832
NlaIII CATG 2 cut(s) 80, 256
NlaIV GGNNCC 4 cut(s) 533, 575, 605, 641
NsiI ATGCAT 1 cut(s) 114
OliI CACNNNNGTG 1 cut(s) 32
PaeR7I CTCGAG 1 cut(s) 366
PctI GAATGC 1 cut(s) 307
PfeI GAWTC 1 cut(s) 588
PinAI ACCGGT 1 cut(s) 340
PkrI GCNGC 2 cut(s) 140, 465
PleI GAGTC 2 cut(s) 658, 797
PpsI GAGTC 2 cut(s) 658, 797
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
PspN4I GGNNCC 4 cut(s) 533, 575, 605, 641
PspPI GGNCC 2 cut(s) 63, 532
PspXI VCTCGAGB 1 cut(s) 366
RsaI GTAC 4 cut(s) 5, 147, 171, 474
RsaNI GTAC 4 cut(s) 4, 146, 170, 473
RseI CAYNNNNRTG 2 cut(s) 32, 579
SatI GCNGC 2 cut(s) 139, 464
Sau3AI GATC 2 cut(s) 763, 832
Sau96I GGNCC 2 cut(s) 63, 532
ScaI AGTACT 2 cut(s) 147, 474
SchI GAGTC 2 cut(s) 659, 797
ScrFI CCNGG 2 cut(s) 264, 578
SduI GDGCHC 2 cut(s) 188, 546
SfaNI GCATC 5 cut(s) 81, 99, 352, 496, 571
Sfr274I CTCGAG 1 cut(s) 366
SlaI CTCGAG 1 cut(s) 366
SmiMI CAYNNNNRTG 2 cut(s) 32, 579
SmlI CTYRAG 2 cut(s) 197, 366
SmoI CTYRAG 2 cut(s) 197, 366
Sse9I AATT 6 cut(s) 30, 52, 165, 205, 666, 671
SsiI CCGC 1 cut(s) 409
SspMI CTAG 2 cut(s) 656, 901
StyD4I CCNGG 2 cut(s) 262, 576
StyI CCWWGG 3 cut(s) 229, 417, 655
TaaI ACNGT 1 cut(s) 494
TaiI ACGT 2 cut(s) 387, 636
TaqI TCGA 1 cut(s) 367
TasI AATT 6 cut(s) 30, 52, 165, 205, 666, 671
TatI WGTACW 3 cut(s) 3, 145, 472
TfiI GAWTC 1 cut(s) 588
TscAI CASTG 2 cut(s) 111, 324
TseI GCWGC 2 cut(s) 138, 463
TspDTI ATGAA 1 cut(s) 269
TspRI CASTG 2 cut(s) 111, 324
XagI CCTNNNNNAGG 1 cut(s) 127
XapI RAATTY 2 cut(s) 52, 666
XhoI CTCGAG 1 cut(s) 366
XmaJI CCTAGG 1 cut(s) 655
XmiI GTMKAC 1 cut(s) 84
XspI CTAG 2 cut(s) 656, 901
ZrmI AGTACT 2 cut(s) 147, 474
Zsp2I ATGCAT 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.