pycom15g32770

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001698
Physical Location & Seq
Forward (+)
31974979 .. 31979977
4999 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g32770.9

Sequence Viewer

Length: 1458 bp
ATGAATGTCTTGAATTACGCTCACTGTTTCTGTTTGTTTCTTGGGAAAATTCTACACACTGTTTTTCTCAACCCGCCAGCTGAATTCCCGCCCCCGGAAGATAATTCCAGGGCGCAGTTTCAGAACGACGAGCTGGAGTACGTCGTCGACGACTACTTCGACATCGCCGACTTCGAAGACACCGACGCTTTTGCCGGTTACCAGCTAAGGGGAAGCCACGGCGCCGACTCGGACTTTGACGACGACGACTTCGCTGAGAGCAAGGTGAAGACTGATACTTCGGCAATGGAAGCTCGAAATGGGAAGGACATACAGGGGATTCCGTGGGAGAGGCTTAACTTCACCAGAGATAGGTACCGTGAAACCCGATTGAAGCAGTACAAGAACTACGAAAGCCTCTCTCTGCCTCGCCAGGACCTCGAAAAGGAGTGCTTGCCAGTAGAGATGGGGAAGACCTTCTATGACTTTCACTACAATACAAGGATTGTCAAGTCCACAATTGTGCATTTCCAGCTGAGGAATCTGTTATGGGCCACATCAAAGCACGATGTATACCTTATGCAGAACTTTTCAGTAATGCATTGGTCTTCCCTACTCAGGAGAGGCAAAGAAGTATTGAACGTATCAAAACCTGTTGTCCCTACTGAGAAACGACCTGGGTTGTTGGTTCGTCCACTCTCTAGAGTGCAAATAAGCACTATGGCTGTGAAGGAAAATTTATTGGTTGCTGGGGGATTCCAAGGTGAACTAATCTGCAAGCACATTAATCAACCTGGAGTTGCTTTCTGCTCAAAATTAACGACAGATGACAATGCCATCACAAATGCAGTGGACATTTTCCAAAACCCTTCTGGCTCAATGAGGGTAATGACTGCAAACAATGATGCTCAAGTTAGAGTTTTTGATGTCCAAAATTTCACTTGCCTCACTCGCTTCTCGTTTGATTGGTCCGTGAATAACACCTCTGTCAGTCCAGATGGTAGGCTGGTTGCAATACTCGGAGATAGTGCGGACTGCTTGATTGCTGATGCTCAGTCTGGAAAAGCTGTCGGAAGCCTCAAAGGTCATTTGGATTATTGCTTTTCCTCAGCATGGCATCCGAACGGACAAGTTTTGGCTACCGGGAATCAAGACACCACTTGCAGGTTATGGGACATAAGGAACCTCTCAGAGTCGCTAGCCGTGTTGAAGGGAAGAATGGGAGCAATACGGGCCATAAAGTTCACTTCGGATGGTCGGTTTATGGCCATGGCTGAGCCGGCAGACTTTGTTCACATCCTTGACACTCAGTCTGGGTATATGAAGGAACAAGAGATTGACCTGTTTGGTGAAATCGCCGGAATATCGTTTAGCCCCGACACAGAAGCTCTGTTTGTTGGGGTCGCTGATCGGACGTATGGTAGCGTGTTAGAGTTCAACAAGAGGCATCATAACCGATATCTCGAAGCAGTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

486

Amino Acids

54.77

Weight (kDa)

5.43

Isoelectric Point (pI)

32.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1134
Acc65I GGTACC 1 cut(s) 354
AccB1I GGYRCC 2 cut(s) 221, 354
AccI GTMKAC 2 cut(s) 147, 552
AciI CCGC 3 cut(s) 74, 89, 1010
AcoI YGGCCR 1 cut(s) 1245
AcsI RAATTY 4 cut(s) 48, 83, 715, 913
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 3 cut(s) 140, 356, 380
AfiI CCNNNNNNNGG 7 cut(s) 94, 208, 351, 424, 597, 1092, 1143
AgsI TTSAA 5 cut(s) 13, 373, 619, 1189, 1417
AhdI GACNNNNNGTC 1 cut(s) 1288
AjnI CCWGG 4 cut(s) 107, 411, 655, 772
AleI CACNNNNGTG 1 cut(s) 500
AluBI AGCT 7 cut(s) 80, 133, 205, 293, 514, 1046, 1367
AluI AGCT 7 cut(s) 80, 133, 205, 293, 514, 1046, 1367
AoxI GGCC 3 cut(s) 531, 1212, 1245
ApoI RAATTY 4 cut(s) 48, 83, 715, 913
ArsI GACNNNNNNTTYG 8 cut(s) 140, 172, 218, 233, 250, 262, 265, 294
AseI ATTAAT 1 cut(s) 765
Asp700I GAANNNNTTC 1 cut(s) 455
Asp718I GGTACC 1 cut(s) 354
AspLEI GCGC 2 cut(s) 115, 224
AspS9I GGNCC 4 cut(s) 415, 531, 948, 1212
AsuC2I CCSGG 2 cut(s) 95, 1123
AsuHPI GGTGA 4 cut(s) 277, 334, 755, 1340
AsuII TTCGAA 1 cut(s) 174
AsuNHI GCTAGC 1 cut(s) 1177
AvaII GGWCC 2 cut(s) 415, 948
BalI TGGCCA 1 cut(s) 1247
BanI GGYRCC 2 cut(s) 221, 354
BbsI GAAGAC 5 cut(s) 183, 275, 458, 579, 1444
BbvCI CCTCAGC 2 cut(s) 515, 1087
BccI CCATC 4 cut(s) 439, 824, 971, 1226
BceAI ACGGC 2 cut(s) 235, 1166
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BciT130I CCWGG 4 cut(s) 109, 413, 657, 774
BcnI CCSGG 2 cut(s) 95, 1123
BfaI CTAG 3 cut(s) 681, 1178, 1456
BfoI RGCGCY 1 cut(s) 225
BfuAI ACCTGC 1 cut(s) 1134
BlpI GCTNAGC 1 cut(s) 1254
Bme1390I CCNGG 6 cut(s) 95, 109, 413, 657, 774, 1123
Bme18I GGWCC 2 cut(s) 415, 948
BmeRI GACNNNNNGTC 1 cut(s) 1288
BmgT120I GGNCC 4 cut(s) 415, 531, 948, 1212
BmiI GGNNCC 3 cut(s) 223, 356, 1163
BmrFI CCNGG 6 cut(s) 95, 109, 413, 657, 774, 1123
BmsI GCATC 4 cut(s) 874, 1018, 1105, 1435
BmtI GCTAGC 1 cut(s) 1181
BpiI GAAGAC 5 cut(s) 183, 275, 458, 579, 1444
BpmI CTGGAG 2 cut(s) 155, 795
Bpu10I CCTNAGC 3 cut(s) 206, 515, 1087
Bpu1102I GCTNAGC 1 cut(s) 1254
Bpu14I TTCGAA 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 873
BpuMI CCSGG 2 cut(s) 95, 1123
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 7 cut(s) 93, 108, 217, 323, 656, 739, 1248
Bsc4I CCNNNNNNNGG 7 cut(s) 94, 208, 351, 424, 597, 1092, 1143
Bse118I RCCGGY 2 cut(s) 194, 1258
Bse1I ACTGG 1 cut(s) 437
Bse3DI GCAATG 1 cut(s) 291
BseBI CCWGG 4 cut(s) 109, 413, 657, 774
BseDI CCNNGG 7 cut(s) 93, 108, 217, 323, 656, 739, 1248
BseGI GGATG 3 cut(s) 1096, 1237, 1275
BseLI CCNNNNNNNGG 7 cut(s) 94, 208, 351, 424, 597, 1092, 1143
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 9 cut(s) 246, 506, 610, 636, 1046, 1101, 1182, 1245, 1301
BseNI ACTGG 1 cut(s) 437
BseYI CCCAGC 1 cut(s) 728
BshFI GGCC 3 cut(s) 533, 1214, 1247
BshNI GGYRCC 2 cut(s) 221, 354
BsiSI CCGG 5 cut(s) 95, 195, 1122, 1259, 1338
BslFI GGGAC 2 cut(s) 623, 1166
BslI CCNNNNNNNGG 7 cut(s) 94, 208, 351, 424, 597, 1092, 1143
BsmFI GGGAC 2 cut(s) 623, 1166
BsnI GGCC 3 cut(s) 533, 1214, 1247
Bsp119I TTCGAA 1 cut(s) 174
Bsp143I GATC 1 cut(s) 1387
Bsp1720I GCTNAGC 1 cut(s) 1254
Bsp19I CCATGG 1 cut(s) 1248
BspACI CCGC 3 cut(s) 74, 89, 1010
BspANI GGCC 3 cut(s) 533, 1214, 1247
BspCNI CTCAG 9 cut(s) 247, 507, 609, 637, 1045, 1100, 1181, 1246, 1300
BspLI GGNNCC 3 cut(s) 223, 356, 1163
BspMI ACCTGC 1 cut(s) 1134
BspOI GCTAGC 1 cut(s) 1181
BspT104I TTCGAA 1 cut(s) 174
BspT107I GGYRCC 2 cut(s) 221, 354
BsrDI GCAATG 1 cut(s) 291
BsrFI RCCGGY 2 cut(s) 194, 1258
BsrI ACTGG 1 cut(s) 437
BssAI RCCGGY 2 cut(s) 194, 1258
BssECI CCNNGG 7 cut(s) 93, 108, 217, 323, 656, 739, 1248
BssMI GATC 1 cut(s) 1387
BssNAI GTATAC 1 cut(s) 553
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 2 cut(s) 739, 1248
Bst1107I GTATAC 1 cut(s) 553
Bst2UI CCWGG 4 cut(s) 109, 413, 657, 774
Bst4CI ACNGT 3 cut(s) 26, 61, 359
BstACI GRCGYC 1 cut(s) 222
BstBI TTCGAA 1 cut(s) 174
BstC8I GCNNGC 5 cut(s) 78, 434, 758, 1179, 1260
BstDSI CCRYGG 3 cut(s) 217, 323, 1248
BstEII GGTNACC 1 cut(s) 197
BstENI CCTNNNNNAGG 1 cut(s) 422
BstF5I GGATG 3 cut(s) 1096, 1237, 1275
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 2 cut(s) 115, 224
BstKTI GATC 1 cut(s) 1390
BstMBI GATC 1 cut(s) 1387
BstMWI GCNNNNNNNGC 5 cut(s) 290, 511, 930, 1211, 1259
BstNI CCWGG 4 cut(s) 109, 413, 657, 774
BstPI GGTNACC 1 cut(s) 197
BstSCI CCNGG 6 cut(s) 93, 107, 411, 655, 772, 1121
BstV2I GAAGAC 5 cut(s) 183, 275, 458, 579, 1444
BstZ17I GTATAC 1 cut(s) 553
BsuRI GGCC 3 cut(s) 533, 1214, 1247
BtgI CCRYGG 3 cut(s) 217, 323, 1248
BtgZI GCGATG 1 cut(s) 148
BtsCI GGATG 3 cut(s) 1096, 1237, 1275
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 3 cut(s) 22, 57, 834
BveI ACCTGC 1 cut(s) 1134
Cac8I GCNNGC 5 cut(s) 78, 434, 758, 1179, 1260
CfoI GCGC 2 cut(s) 115, 224
Cfr10I RCCGGY 2 cut(s) 194, 1258
Cfr13I GGNCC 4 cut(s) 415, 531, 948, 1212
CseI GACGC 1 cut(s) 194
Csp6I GTAC 3 cut(s) 139, 355, 379
CspCI CAANNNNNGTGG 2 cut(s) 810, 845
CviAII CATG 2 cut(s) 1092, 1249
CviQI GTAC 3 cut(s) 139, 355, 379
DinI GGCGCC 1 cut(s) 223
DpnI GATC 1 cut(s) 1389
DpnII GATC 1 cut(s) 1387
DriI GACNNNNNGTC 1 cut(s) 1288
EaeI YGGCCR 1 cut(s) 1245
Eam1105I GACNNNNNGTC 1 cut(s) 1288
Eco130I CCWWGG 2 cut(s) 739, 1248
Eco32I GATATC 1 cut(s) 1439
Eco47I GGWCC 2 cut(s) 415, 948
Eco91I GGTNACC 1 cut(s) 197
EcoNI CCTNNNNNAGG 1 cut(s) 422
EcoO109I RGGNCCY 1 cut(s) 415
EcoO65I GGTNACC 1 cut(s) 197
EcoRI GAATTC 1 cut(s) 83
EcoRII CCWGG 4 cut(s) 107, 411, 655, 772
EcoRV GATATC 1 cut(s) 1439
EcoT14I CCWWGG 2 cut(s) 739, 1248
EcoT22I ATGCAT 1 cut(s) 582
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
ErhI CCWWGG 2 cut(s) 739, 1248
FaeI CATG 2 cut(s) 1095, 1252
FalI AAGNNNNNCTT 2 cut(s) 416, 448
FaqI GGGAC 2 cut(s) 623, 1166
FatI CATG 2 cut(s) 1091, 1248
FauI CCCGC 2 cut(s) 81, 96
FblI GTMKAC 2 cut(s) 147, 552
FokI GGATG 3 cut(s) 1083, 1244, 1262
FspBI CTAG 3 cut(s) 681, 1178, 1456
GlaI GCGC 2 cut(s) 114, 223
GsaI CCCAGC 1 cut(s) 732
GsuI CTGGAG 2 cut(s) 155, 795
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 3 cut(s) 533, 1214, 1247
HapII CCGG 5 cut(s) 95, 195, 1122, 1259, 1338
HgaI GACGC 1 cut(s) 194
HhaI GCGC 2 cut(s) 115, 224
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 2 cut(s) 1095, 1252
Hin6I GCGC 2 cut(s) 113, 222
HinP1I GCGC 2 cut(s) 113, 222
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 6 cut(s) 227, 319, 520, 735, 1126, 1172
HpaII CCGG 5 cut(s) 95, 195, 1122, 1259, 1338
HphI GGTGA 4 cut(s) 277, 334, 755, 1340
Hpy166II GTNNAC 8 cut(s) 148, 495, 553, 674, 746, 832, 1224, 1273
Hpy188I TCNGA 8 cut(s) 123, 232, 1001, 1052, 1101, 1171, 1231, 1392
Hpy188III TCNNGA 7 cut(s) 10, 598, 681, 974, 1038, 1130, 1442
Hpy8I GTNNAC 8 cut(s) 148, 495, 553, 674, 746, 832, 1224, 1273
Hpy99I CGWCG 7 cut(s) 131, 146, 149, 152, 188, 245, 248
HpyAV CCTTC 6 cut(s) 298, 466, 703, 858, 1183, 1297
HpyCH4III ACNGT 3 cut(s) 26, 61, 359
HpyCH4IV ACGT 3 cut(s) 141, 621, 1394
HpyCH4V TGCA 9 cut(s) 505, 562, 580, 688, 756, 827, 875, 992, 1143
HpyF10VI GCNNNNNNNGC 5 cut(s) 290, 511, 930, 1211, 1259
HpySE526I ACGT 3 cut(s) 141, 621, 1394
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 2 cut(s) 1095, 1252
HspAI GCGC 2 cut(s) 113, 222
KasI GGCGCC 1 cut(s) 221
KpnI GGTACC 1 cut(s) 358
KroI GCCGGC 1 cut(s) 1258
KroNI GCCGGC 1 cut(s) 1260
Kzo9I GATC 1 cut(s) 1387
LmnI GCTCC 1 cut(s) 1202
LweI GCATC 4 cut(s) 874, 1018, 1105, 1435
MaeI CTAG 3 cut(s) 681, 1178, 1456
MaeII ACGT 3 cut(s) 141, 621, 1394
MaeIII GTNAC 1 cut(s) 197
MalI GATC 1 cut(s) 1389
MboI GATC 1 cut(s) 1387
MboII GAAGA 7 cut(s) 110, 188, 280, 463, 579, 1206, 1444
MfeI CAATTG 1 cut(s) 498
MlsI TGGCCA 1 cut(s) 1247
MluCI AATT 8 cut(s) 13, 48, 83, 103, 498, 715, 794, 913
MluNI TGGCCA 1 cut(s) 1247
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 2 cut(s) 221, 1181
MmeI TCCRAC 1 cut(s) 1030
Mox20I TGGCCA 1 cut(s) 1247
Mph1103I ATGCAT 1 cut(s) 582
MroNI GCCGGC 1 cut(s) 1258
MroXI GAANNNNTTC 1 cut(s) 455
MscI TGGCCA 1 cut(s) 1247
MseI TTAA 3 cut(s) 336, 765, 797
MslI CAYNNNNRTG 1 cut(s) 500
Msp20I TGGCCA 1 cut(s) 1247
MspA1I CMGCKG 2 cut(s) 80, 514
MspI CCGG 5 cut(s) 95, 195, 1122, 1259, 1338
MspR9I CCNGG 6 cut(s) 95, 109, 413, 657, 774, 1123
MunI CAATTG 1 cut(s) 498
MvaI CCWGG 4 cut(s) 109, 413, 657, 774
MwoI GCNNNNNNNGC 5 cut(s) 290, 511, 930, 1211, 1259
NaeI GCCGGC 1 cut(s) 1260
NarI GGCGCC 1 cut(s) 222
NciI CCSGG 2 cut(s) 95, 1123
NcoI CCATGG 1 cut(s) 1248
NdeII GATC 1 cut(s) 1387
NgoMIV GCCGGC 1 cut(s) 1258
NheI GCTAGC 1 cut(s) 1177
NlaIII CATG 2 cut(s) 1095, 1252
NlaIV GGNNCC 3 cut(s) 223, 356, 1163
NsiI ATGCAT 1 cut(s) 582
NspV TTCGAA 1 cut(s) 174
OliI CACNNNNGTG 1 cut(s) 500
PcsI WCGNNNNNNNCGW 5 cut(s) 147, 156, 165, 171, 180
PdiI GCCGGC 1 cut(s) 1260
PdmI GAANNNNTTC 1 cut(s) 455
PfeI GAWTC 4 cut(s) 319, 520, 735, 1126
PleI GAGTC 2 cut(s) 221, 1180
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 2 cut(s) 221, 1180
PpuMI RGGWCCY 1 cut(s) 415
PshBI ATTAAT 1 cut(s) 765
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 4 cut(s) 107, 411, 655, 772
PspEI GGTNACC 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 728
PspGI CCWGG 4 cut(s) 107, 411, 655, 772
PspN4I GGNNCC 3 cut(s) 223, 356, 1163
PspPI GGNCC 4 cut(s) 415, 531, 948, 1212
PspPPI RGGWCCY 1 cut(s) 415
PvuII CAGCTG 2 cut(s) 80, 514
RsaI GTAC 3 cut(s) 140, 356, 380
RsaNI GTAC 3 cut(s) 139, 355, 379
RseI CAYNNNNRTG 1 cut(s) 500
SalI GTCGAC 1 cut(s) 146
SaqAI TTAA 3 cut(s) 336, 765, 797
Sau3AI GATC 1 cut(s) 1387
Sau96I GGNCC 4 cut(s) 415, 531, 948, 1212
SchI GAGTC 2 cut(s) 221, 1181
ScrFI CCNGG 6 cut(s) 95, 109, 413, 657, 774, 1123
SfaNI GCATC 4 cut(s) 874, 1018, 1105, 1435
SfoI GGCGCC 1 cut(s) 223
SfuI TTCGAA 1 cut(s) 174
SgrDI CGTCGACG 1 cut(s) 146
SinI GGWCC 2 cut(s) 415, 948
SmiMI CAYNNNNRTG 1 cut(s) 500
SmlI CTYRAG 1 cut(s) 888
SmoI CTYRAG 1 cut(s) 888
Sse9I AATT 8 cut(s) 13, 48, 83, 103, 498, 715, 794, 913
SsiI CCGC 3 cut(s) 74, 89, 1010
SspDI GGCGCC 1 cut(s) 221
SspMI CTAG 3 cut(s) 681, 1178, 1456
StyD4I CCNGG 6 cut(s) 93, 107, 411, 655, 772, 1121
StyI CCWWGG 2 cut(s) 739, 1248
TaaI ACNGT 3 cut(s) 26, 61, 359
TaiI ACGT 3 cut(s) 144, 624, 1397
TaqI TCGA 6 cut(s) 147, 159, 174, 295, 420, 1443
TasI AATT 8 cut(s) 13, 48, 83, 103, 498, 715, 794, 913
TatI WGTACW 1 cut(s) 378
TfiI GAWTC 4 cut(s) 319, 520, 735, 1126
Tru1I TTAA 3 cut(s) 336, 765, 797
Tru9I TTAA 3 cut(s) 336, 765, 797
TscAI CASTG 3 cut(s) 29, 64, 834
TspDTI ATGAA 2 cut(s) 17, 1316
TspGWI ACGGA 3 cut(s) 312, 940, 1119
TspRI CASTG 3 cut(s) 29, 64, 834
VpaK11BI GGWCC 2 cut(s) 415, 948
VspI ATTAAT 1 cut(s) 765
XagI CCTNNNNNAGG 1 cut(s) 422
XapI RAATTY 4 cut(s) 48, 83, 715, 913
XbaI TCTAGA 1 cut(s) 680
XcmI CCANNNNNNNNNTGG 1 cut(s) 848
XmiI GTMKAC 2 cut(s) 147, 552
XmnI GAANNNNTTC 1 cut(s) 455
XspI CTAG 3 cut(s) 681, 1178, 1456
Zsp2I ATGCAT 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.