Rroxscaffold_3G00220800

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001698
Physical Location & Seq
Forward (+)
3187889 .. 3256872
68984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00220800.1

Sequence Viewer

Length: 1563 bp
ATGGTTCAGCCCACCTCGCTTTTTTTTCGGCACAATCCAATTCTCGCTTTCTGGATTCTTCTCCCTCCTTTCACTTTTCTACATCATCATCCTCAGATTCCTTTGCTCTCTCCAAAAGAAAGCACTTTTCTACTTGTTTTCCACACACTTCCTACCTCATCAGCTCTGTTCCTCCAATTCCAGGTTTCAATGGCGTATTTCCAGAACGATGACCTCGAGTACGTCGTCAACGACTACTACGGCGACGTCGTTGAGTTTGAGGAAGACAACGACGCTTTTGCTGATAACCAGCCCCGCCCAAGCCTCGAGGTAGTAGACGATGACTCCGACGACGACGACTACTTTGAAGACGACCTTGATTCGAGCAAGGTGAAGAGTGATACATCGGCTTTGGAAGCTAAAAATGGAAAGGATATACAGGGAATACCATGGGAGAGGCTTAATTTTACCAGAGATAAGTACCGTGAGGCGCGATTGAAGCAGTATAAGAACTATGAGAGCCTCGGTCGTCCTCGTCAAGACCTCGAGAAGGAGTGCTTGCAAGTGGAGAAGGGGAAGAGTTTCTATGACTTTCAATACAACACAAGGCTTGTGAAGTCGACAATTGTTCATTTTCAGCTAAGGAATTTGTTATGGGCCACATCAAAGCATGATGTATACCTGATGCAGAACTTCTCAGTGATGCATTGGAACTCTTTGCACAGGAAAGGCAGCGAAGTACTCAATGTGGCCAAACCAATTGTACCTACCGAGAAACAACCTGGGTTGTTGGCACGACCACTCTCTAGAGTGCAGATAAGCACTATGGCTGTCAAGGACAATTTGTTGGTTGCTGGGGGCTTCCAAGGTGAACTAATCTGCAAGCACATGAAACAACCTGGAGTTGCTTTCTGCTCAAAACTAACAACAGATGAGAATGCCATCACTAATGCAGTGGATATTTTCCAAAATCCAACTGGCTCAGTGAGGGTAATGACTGCAAATAATGATGCTCAAGTTAGAGTTTTTGACGTTGAAAAGTTCGCTTGCCTCAACCGCTTCTCCTTCGAGTGGTCTGTAAATAACACCTCTGTTAGTCCTGATGGTAAGCTGCTCGCAGTACTTGGTGATAGTGCAGACTGTTTGATAGCTGATGCTCAGTCTGGAAAAGCTGTCGGGGGCCTGAAAGGGCACTTAGACTATTGCTTTTCTTCAGCTTGGCACCCGGATGGACGAATCTTGGCTACTGGGAACCAAGACACTACCTGTAGGCTATGGGACATAAGGAACCTGTCAGAGTCCCTAGCCGTGTTGAAGGGAAGAATGGGAGCAATAAGAGCTATAAAATTCACCTCTGATGGTCGTTTTATGGCCATGGCTGAGCCAGCAGACTTTGTTCACATCATTGATACCCAGTCCGGATATGCAAAGGGACAAGAGATTGATCTGTTTGGTGAGATTGCCGGAATATCCTTCAGCCCTGACTCTGAAGCTTTGTTTGTTGGGGTTGCTGATCGCACATATGGTAGTGTATTGGAGTTCAATAAGAGGCACTATAATCCCTATCAAGAAATAGTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

520

Amino Acids

58.77

Weight (kDa)

5.31

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 269 - 465 1.3e-18 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 316 - 492 2.6e-26 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 321 - 453 4.6e-12 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 322 - 426 1.1e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 323 - 500 3.6e-20 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 323 - 467 1.3e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 324 - 428 1e-06 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 329 - 500 3.6e-13 WDR3 first beta-propeller domain
WDR55 PF24796 353 - 496 1.4e-06 WDR55
WD40 PF00400 383 - 420 3.1e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 249
AbsI CCTCGAGG 1 cut(s) 305
AccB1I GGYRCC 1 cut(s) 1200
AccI GTMKAC 3 cut(s) 315, 599, 657
AccII CGCG 1 cut(s) 472
AccIII TCCGGA 1 cut(s) 1397
AciI CCGC 2 cut(s) 295, 1036
AcoI YGGCCR 2 cut(s) 729, 1350
AcsI RAATTY 2 cut(s) 625, 1325
AcuI CTGAAG 3 cut(s) 1176, 1438, 1488
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 5 cut(s) 221, 461, 720, 744, 1101
AfiI CCNNNNNNNGG 3 cut(s) 181, 529, 1050
AgsI TTSAA 7 cut(s) 189, 347, 478, 575, 1016, 1294, 1522
AjnI CCWGG 3 cut(s) 180, 760, 877
AluBI AGCT 9 cut(s) 164, 398, 619, 1090, 1130, 1151, 1196, 1319, 1472
AluI AGCT 9 cut(s) 164, 398, 619, 1090, 1130, 1151, 1196, 1319, 1472
Ama87I CYCGRG 3 cut(s) 215, 305, 524
Aor13HI TCCGGA 1 cut(s) 1397
AoxI GGCC 4 cut(s) 636, 729, 1159, 1350
ApeKI GCWGC 2 cut(s) 711, 1090
ApoI RAATTY 2 cut(s) 625, 1325
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
Asp700I GAANNNNTTC 1 cut(s) 560
AspLEI GCGC 1 cut(s) 472
AspS9I GGNCC 2 cut(s) 636, 1159
AsuC2I CCSGG 1 cut(s) 1205
AsuHPI GGTGA 5 cut(s) 382, 860, 1118, 1321, 1445
AvaI CYCGRG 3 cut(s) 215, 305, 524
BaeGI GKGCMC 1 cut(s) 1173
BalI TGGCCA 2 cut(s) 731, 1352
BanI GGYRCC 1 cut(s) 1200
BbsI GAAGAC 3 cut(s) 270, 354, 1549
BbvI GCAGC 2 cut(s) 723, 1077
BccI CCATC 4 cut(s) 929, 1076, 1202, 1331
BceAI ACGGC 2 cut(s) 256, 1271
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BciT130I CCWGG 3 cut(s) 182, 762, 879
BcnI CCSGG 1 cut(s) 1205
BfaI CTAG 3 cut(s) 786, 1283, 1561
BfmI CTRYAG 1 cut(s) 1246
BisI GCNGC 2 cut(s) 712, 1091
BlpI GCTNAGC 1 cut(s) 1359
BlsI GCNGC 2 cut(s) 713, 1092
BmcAI AGTACT 2 cut(s) 720, 1101
Bme1390I CCNGG 4 cut(s) 182, 762, 879, 1205
BmeT110I CYCGRG 3 cut(s) 215, 305, 524
BmgT120I GGNCC 2 cut(s) 636, 1159
BmiI GGNNCC 4 cut(s) 1160, 1202, 1232, 1268
BmrFI CCNGG 4 cut(s) 182, 762, 879, 1205
BmrI ACTGGG 2 cut(s) 1236, 1387
BmsI GCATC 4 cut(s) 654, 672, 979, 1123
BmuI ACTGGG 2 cut(s) 1236, 1387
BpiI GAAGAC 3 cut(s) 270, 354, 1549
BpmI CTGGAG 1 cut(s) 900
Bpu10I CCTNAGC 1 cut(s) 620
Bpu1102I GCTNAGC 1 cut(s) 1359
BpuEI CTTGAG 1 cut(s) 978
BpuMI CCSGG 1 cut(s) 1205
BsaHI GRCGYC 1 cut(s) 246
BsaJI CCNNGG 5 cut(s) 428, 502, 761, 844, 1353
BsaWI WCCGGW 1 cut(s) 1397
BsaXI ACNNNNNCTCC 4 cut(s) 308, 338, 1025, 1055
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 529, 1050
Bse1I ACTGG 3 cut(s) 961, 1231, 1393
BseAI TCCGGA 1 cut(s) 1397
BseBI CCWGG 3 cut(s) 182, 762, 879
BseDI CCNNGG 5 cut(s) 428, 502, 761, 844, 1353
BseGI GGATG 2 cut(s) 88, 1213
BseLI CCNNNNNNNGG 3 cut(s) 181, 529, 1050
BseMII CTCAG 5 cut(s) 107, 690, 975, 1151, 1350
BseNI ACTGG 3 cut(s) 961, 1231, 1393
BseSI GKGCMC 1 cut(s) 1173
BseXI GCAGC 2 cut(s) 723, 1077
BseYI CCCAGC 1 cut(s) 833
BsgI GTGCAG 2 cut(s) 812, 1134
Bsh1236I CGCG 1 cut(s) 472
Bsh1285I CGRYCG 1 cut(s) 508
BshFI GGCC 4 cut(s) 638, 731, 1161, 1352
BshNI GGYRCC 1 cut(s) 1200
BsiEI CGRYCG 1 cut(s) 508
BsiHKCI CYCGRG 3 cut(s) 215, 305, 524
BsiSI CCGG 3 cut(s) 1205, 1398, 1443
BslFI GGGAC 3 cut(s) 1264, 1271, 1425
BslI CCNNNNNNNGG 3 cut(s) 181, 529, 1050
BsmFI GGGAC 3 cut(s) 1264, 1271, 1425
BsmI GAATGC 1 cut(s) 922
BsnI GGCC 4 cut(s) 638, 731, 1161, 1352
BsoBI CYCGRG 3 cut(s) 215, 305, 524
Bsp1286I GDGCHC 1 cut(s) 1173
Bsp13I TCCGGA 1 cut(s) 1397
Bsp143I GATC 2 cut(s) 1423, 1492
Bsp1720I GCTNAGC 1 cut(s) 1359
Bsp19I CCATGG 2 cut(s) 428, 1353
BspACI CCGC 2 cut(s) 295, 1036
BspANI GGCC 4 cut(s) 638, 731, 1161, 1352
BspCNI CTCAG 5 cut(s) 106, 689, 974, 1150, 1351
BspEI TCCGGA 1 cut(s) 1397
BspFNI CGCG 1 cut(s) 472
BspLI GGNNCC 4 cut(s) 1160, 1202, 1232, 1268
BspT107I GGYRCC 1 cut(s) 1200
BsrI ACTGG 3 cut(s) 961, 1231, 1393
BssECI CCNNGG 5 cut(s) 428, 502, 761, 844, 1353
BssMI GATC 2 cut(s) 1423, 1492
BssNAI GTATAC 1 cut(s) 658
BssNI GRCGYC 1 cut(s) 246
BssT1I CCWWGG 3 cut(s) 428, 844, 1353
Bst1107I GTATAC 1 cut(s) 658
Bst2UI CCWGG 3 cut(s) 182, 762, 879
Bst4CI ACNGT 2 cut(s) 464, 1121
Bst6I CTCTTC 2 cut(s) 368, 551
BstACI GRCGYC 1 cut(s) 246
BstC8I GCNNGC 5 cut(s) 539, 863, 1027, 1095, 1365
BstDEI CTNAG 7 cut(s) 93, 620, 676, 961, 1137, 1174, 1359
BstDSI CCRYGG 2 cut(s) 428, 1353
BstENI CCTNNNNNAGG 1 cut(s) 527
BstF5I GGATG 2 cut(s) 88, 1213
BstFNI CGCG 1 cut(s) 472
BstHHI GCGC 1 cut(s) 472
BstKTI GATC 2 cut(s) 1426, 1495
BstMBI GATC 2 cut(s) 1423, 1492
BstMCI CGRYCG 1 cut(s) 508
BstMWI GCNNNNNNNGC 6 cut(s) 16, 395, 478, 1035, 1316, 1364
BstNI CCWGG 3 cut(s) 182, 762, 879
BstSCI CCNGG 4 cut(s) 180, 760, 877, 1203
BstSFI CTRYAG 1 cut(s) 1246
BstSLI GKGCMC 1 cut(s) 1173
BstUI CGCG 1 cut(s) 472
BstV1I GCAGC 2 cut(s) 723, 1077
BstV2I GAAGAC 3 cut(s) 270, 354, 1549
BstZ17I GTATAC 1 cut(s) 658
BsuRI GGCC 4 cut(s) 638, 731, 1161, 1352
BtgI CCRYGG 2 cut(s) 428, 1353
BtsCI GGATG 2 cut(s) 88, 1213
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 3 cut(s) 684, 939, 969
Cac8I GCNNGC 5 cut(s) 539, 863, 1027, 1095, 1365
CfoI GCGC 1 cut(s) 472
Cfr13I GGNCC 2 cut(s) 636, 1159
CseI GACGC 1 cut(s) 281
Csp6I GTAC 5 cut(s) 220, 460, 719, 743, 1100
CviAII CATG 4 cut(s) 429, 650, 868, 1354
CviQI GTAC 5 cut(s) 220, 460, 719, 743, 1100
DdeI CTNAG 7 cut(s) 93, 620, 676, 961, 1137, 1174, 1359
DpnI GATC 2 cut(s) 1425, 1494
DpnII GATC 2 cut(s) 1423, 1492
EaeI YGGCCR 2 cut(s) 729, 1350
Eam1104I CTCTTC 2 cut(s) 368, 551
EarI CTCTTC 2 cut(s) 368, 551
Eco130I CCWWGG 3 cut(s) 428, 844, 1353
Eco57I CTGAAG 3 cut(s) 1176, 1438, 1488
Eco88I CYCGRG 3 cut(s) 215, 305, 524
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoO109I RGGNCCY 1 cut(s) 1159
EcoRII CCWGG 3 cut(s) 180, 760, 877
EcoT14I CCWWGG 3 cut(s) 428, 844, 1353
EcoT22I ATGCAT 1 cut(s) 687
ErhI CCWWGG 3 cut(s) 428, 844, 1353
FaeI CATG 4 cut(s) 432, 653, 871, 1357
FalI AAGNNNNNCTT 4 cut(s) 339, 371, 521, 553
FaqI GGGAC 3 cut(s) 1264, 1271, 1425
FatI CATG 4 cut(s) 428, 649, 867, 1353
FauI CCCGC 1 cut(s) 302
FauNDI CATATG 1 cut(s) 1501
FblI GTMKAC 3 cut(s) 315, 599, 657
Fnu4HI GCNGC 2 cut(s) 712, 1091
FokI GGATG 2 cut(s) 75, 1220
Fsp4HI GCNGC 2 cut(s) 712, 1091
FspBI CTAG 3 cut(s) 786, 1283, 1561
GlaI GCGC 1 cut(s) 471
GluI GCNGC 2 cut(s) 712, 1091
GsaI CCCAGC 1 cut(s) 837
GsuI CTGGAG 1 cut(s) 900
HaeIII GGCC 4 cut(s) 638, 731, 1161, 1352
HapII CCGG 3 cut(s) 1205, 1398, 1443
HgaI GACGC 1 cut(s) 281
HhaI GCGC 1 cut(s) 472
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 4 cut(s) 432, 653, 871, 1357
Hin6I GCGC 1 cut(s) 470
HinP1I GCGC 1 cut(s) 470
HincII GTYRAC 2 cut(s) 229, 600
HindII GTYRAC 2 cut(s) 229, 600
HindIII AAGCTT 1 cut(s) 1470
HinfI GANTC 7 cut(s) 55, 97, 323, 359, 1215, 1277, 1463
HpaII CCGG 3 cut(s) 1205, 1398, 1443
HphI GGTGA 5 cut(s) 382, 860, 1118, 1321, 1445
Hpy166II GTNNAC 6 cut(s) 229, 316, 600, 658, 851, 1378
Hpy188I TCNGA 5 cut(s) 96, 328, 1276, 1336, 1468
Hpy188III TCNNGA 9 cut(s) 52, 202, 518, 526, 786, 1079, 1143, 1398, 1547
Hpy8I GTNNAC 6 cut(s) 229, 316, 600, 658, 851, 1378
Hpy99I CGWCG 7 cut(s) 227, 248, 251, 275, 332, 335, 338
HpyAV CCTTC 5 cut(s) 523, 544, 1054, 1288, 1462
HpyCH4III ACNGT 2 cut(s) 464, 1121
HpyCH4IV ACGT 3 cut(s) 222, 246, 1011
HpyF10VI GCNNNNNNNGC 6 cut(s) 16, 395, 478, 1035, 1316, 1364
HpyF3I CTNAG 7 cut(s) 93, 620, 676, 961, 1137, 1174, 1359
HpySE526I ACGT 3 cut(s) 222, 246, 1011
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 4 cut(s) 432, 653, 871, 1357
HspAI GCGC 1 cut(s) 470
Kpn2I TCCGGA 1 cut(s) 1397
Kzo9I GATC 2 cut(s) 1423, 1492
LmnI GCTCC 1 cut(s) 1307
Lsp1109I GCAGC 2 cut(s) 723, 1077
LweI GCATC 4 cut(s) 654, 672, 979, 1123
MaeI CTAG 3 cut(s) 786, 1283, 1561
MaeII ACGT 3 cut(s) 222, 246, 1011
MalI GATC 2 cut(s) 1425, 1494
MboI GATC 2 cut(s) 1423, 1492
MboII GAAGA 8 cut(s) 50, 275, 359, 385, 568, 1182, 1311, 1549
MfeI CAATTG 2 cut(s) 603, 738
MhlI GDGCHC 1 cut(s) 1173
MlsI TGGCCA 2 cut(s) 731, 1352
MluCI AATT 8 cut(s) 39, 176, 442, 603, 625, 738, 820, 1325
MluNI TGGCCA 2 cut(s) 731, 1352
MlyI GAGTC 3 cut(s) 317, 1286, 1457
MmeI TCCRAC 2 cut(s) 351, 977
Mox20I TGGCCA 2 cut(s) 731, 1352
Mph1103I ATGCAT 1 cut(s) 687
MroI TCCGGA 1 cut(s) 1397
MroXI GAANNNNTTC 1 cut(s) 560
MscI TGGCCA 2 cut(s) 731, 1352
MseI TTAA 1 cut(s) 441
MslI CAYNNNNRTG 1 cut(s) 1206
Msp20I TGGCCA 2 cut(s) 731, 1352
MspI CCGG 3 cut(s) 1205, 1398, 1443
MspR9I CCNGG 4 cut(s) 182, 762, 879, 1205
MunI CAATTG 2 cut(s) 603, 738
Mva1269I GAATGC 1 cut(s) 922
MvaI CCWGG 3 cut(s) 182, 762, 879
MvnI CGCG 1 cut(s) 472
MwoI GCNNNNNNNGC 6 cut(s) 16, 395, 478, 1035, 1316, 1364
NciI CCSGG 1 cut(s) 1205
NcoI CCATGG 2 cut(s) 428, 1353
NdeI CATATG 1 cut(s) 1501
NdeII GATC 2 cut(s) 1423, 1492
NlaIII CATG 4 cut(s) 432, 653, 871, 1357
NlaIV GGNNCC 4 cut(s) 1160, 1202, 1232, 1268
NsiI ATGCAT 1 cut(s) 687
PaeR7I CTCGAG 3 cut(s) 215, 305, 524
PcsI WCGNNNNNNNCGW 5 cut(s) 213, 222, 228, 246, 324
PctI GAATGC 1 cut(s) 922
PdmI GAANNNNTTC 1 cut(s) 560
PfeI GAWTC 4 cut(s) 55, 97, 359, 1215
PkrI GCNGC 2 cut(s) 713, 1092
PleI GAGTC 3 cut(s) 317, 1285, 1457
PpsI GAGTC 3 cut(s) 317, 1285, 1457
Psp6I CCWGG 3 cut(s) 180, 760, 877
PspFI CCCAGC 1 cut(s) 833
PspGI CCWGG 3 cut(s) 180, 760, 877
PspN4I GGNNCC 4 cut(s) 1160, 1202, 1232, 1268
PspPI GGNCC 2 cut(s) 636, 1159
PspXI VCTCGAGB 2 cut(s) 215, 305
RsaI GTAC 5 cut(s) 221, 461, 720, 744, 1101
RsaNI GTAC 5 cut(s) 220, 460, 719, 743, 1100
RseI CAYNNNNRTG 1 cut(s) 1206
SalI GTCGAC 1 cut(s) 598
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 2 cut(s) 712, 1091
Sau3AI GATC 2 cut(s) 1423, 1492
Sau96I GGNCC 2 cut(s) 636, 1159
ScaI AGTACT 2 cut(s) 720, 1101
SchI GAGTC 3 cut(s) 317, 1286, 1457
ScrFI CCNGG 4 cut(s) 182, 762, 879, 1205
SduI GDGCHC 1 cut(s) 1173
SfaNI GCATC 4 cut(s) 654, 672, 979, 1123
SfcI CTRYAG 1 cut(s) 1246
Sfr274I CTCGAG 3 cut(s) 215, 305, 524
SlaI CTCGAG 3 cut(s) 215, 305, 524
SmiMI CAYNNNNRTG 1 cut(s) 1206
SmlI CTYRAG 4 cut(s) 215, 305, 524, 993
SmoI CTYRAG 4 cut(s) 215, 305, 524, 993
Sse9I AATT 8 cut(s) 39, 176, 442, 603, 625, 738, 820, 1325
SsiI CCGC 2 cut(s) 295, 1036
SspMI CTAG 3 cut(s) 786, 1283, 1561
StyD4I CCNGG 4 cut(s) 180, 760, 877, 1203
StyI CCWWGG 3 cut(s) 428, 844, 1353
TaaI ACNGT 2 cut(s) 464, 1121
TaiI ACGT 3 cut(s) 225, 249, 1014
TaqI TCGA 6 cut(s) 216, 306, 362, 525, 599, 1047
TaqII GACCGA 1 cut(s) 494
TasI AATT 8 cut(s) 39, 176, 442, 603, 625, 738, 820, 1325
TatI WGTACW 2 cut(s) 718, 1099
TfiI GAWTC 4 cut(s) 55, 97, 359, 1215
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TscAI CASTG 3 cut(s) 684, 939, 969
TseI GCWGC 2 cut(s) 711, 1090
TspDTI ATGAA 2 cut(s) 599, 884
TspRI CASTG 3 cut(s) 684, 939, 969
XagI CCTNNNNNAGG 1 cut(s) 527
XapI RAATTY 2 cut(s) 625, 1325
XbaI TCTAGA 1 cut(s) 785
XcmI CCANNNNNNNNNTGG 1 cut(s) 953
XhoI CTCGAG 3 cut(s) 215, 305, 524
XmiI GTMKAC 3 cut(s) 315, 599, 657
XmnI GAANNNNTTC 1 cut(s) 560
XspI CTAG 3 cut(s) 786, 1283, 1561
ZraI GACGTC 1 cut(s) 247
ZrmI AGTACT 2 cut(s) 720, 1101
Zsp2I ATGCAT 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.