Rw7G040470

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001698
Physical Location & Seq
Reverse (-)
63009421 .. 63014573
5153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G040470.1

Sequence Viewer

Length: 1161 bp
ATGAGCAATCAGTTGAGAAATAGTACTGTCAGGGGTAAGCATGAGTCCAGTGAAGCTGCTAGGAGTTCTACCTCCGAAGACTTAAAGAGCGGTTTTACATTTAAAGCTGATGGCAGCTCTGAGTCTGACTCGGATTCTGAACTCAGGAGGAAGATCTCTGATACTCATCAGATCGAAATATCTTTAAAGAGTGATACATCGGCTTTGGAAGGTAGAAATGGAATAGACATACAGGGAATACCATGGGAGAGGCTTAATTTCACCGGAGATAAGTACCGTGAAGCGCGATTGAAGCAATATAAGAACTATGAGAGCCTCGGTCGGCCTCGTCAAGACCTTGAGAAGGAGTGCTTGCAAGTGGAGAAGGTGAAGAGTTTCTATGACTTTAACTACAACACAAGGCTTGTCAAGTCCACAATTGTGCATTTTCAGGTAGAGATCATGATCTTGGTTCTCGTAACTAAAGGACCTGGGTTGTTGGCACGACCACTCTCTAGAGTGCAGATAAGCACTATGGCTGTCAAGGAAAATTTGTTGGTTGCTGGGGGCTTCCAAGGTGAACTAATCTGCAAGTTAGGCGGGTGTTATAATAAGTTTTCGACTACTGAATCGAACACCTCTGTTGGTCCCGATGGTAAGCTGCTTGCAGTACTTGGTGATGGTGCAGACTGTTTGATTGCTGCTGCTCTGTCTGGTAAAATGATGTGCAGAGTTCTGATATATTATTATATTTCCTACTTTGTATACGCTGTTGGAGACCTCAAAGGGCACTTAGACTATTGCTTTTCCTCAGCTTGGCACCCGGATGGGCGAATCTTAGCTACTGGGAACCAAGACACTACTTACAGGTTATGGGACATAAGGAACCTGTCAGAGTCCCTAGCCGTGTTGAAGGGAAGAATGGGAGCAATAAGAGCTATAAAATTCACCTCTGATGGTCGTTTTATGGCCATTGCTGAGCCAGCGGACTTTGTTCATATCATTGATACCCAGTGCAGATATGCAAAGGTACAAGAGATTGATCTGTTTGGTGACATTGCCGGAATATCCTTCAGCCCCGACTCTGAAGCTTTGTTTGTTGGGGTTGCTGATCGCACATATGGTAGTGTATTGGAGTTCAATAAGAGGCACTACAATCCCTATCAAGAAATAGTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

386

Amino Acids

43.14

Weight (kDa)

6.67

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 251 - 358 4.3e-16 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 251 - 366 4.8e-11 WDR5 beta-propeller domain
WD40 PF00400 252 - 286 7.7e-06 WD domain, G-beta repeat
WD40_CDC20-Fz PF24807 252 - 311 3e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 254 - 366 7.4e-08 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 261 - 358 1.2e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 263 - 360 4e-11 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 588
AccB1I GGYRCC 1 cut(s) 798
AccBSI CCGCTC 1 cut(s) 90
AccI GTMKAC 1 cut(s) 744
AccII CGCG 1 cut(s) 286
AciI CCGC 3 cut(s) 90, 579, 965
AcoI YGGCCR 1 cut(s) 948
AcsI RAATTY 2 cut(s) 529, 923
AcuI CTGAAG 2 cut(s) 1036, 1086
AfaI GTAC 4 cut(s) 25, 275, 651, 1011
AfiI CCNNNNNNNGG 2 cut(s) 343, 795
AgsI TTSAA 3 cut(s) 292, 892, 1120
AjnI CCWGG 1 cut(s) 469
AleI CACNNNNGTG 1 cut(s) 419
AluBI AGCT 8 cut(s) 56, 107, 117, 640, 794, 821, 917, 1070
AluI AGCT 8 cut(s) 56, 107, 117, 640, 794, 821, 917, 1070
Alw26I GTCTC 1 cut(s) 750
AoxI GGCC 2 cut(s) 323, 948
ApeKI GCWGC 5 cut(s) 56, 114, 640, 680, 683
ApoI RAATTY 2 cut(s) 529, 923
Asp700I GAANNNNTTC 1 cut(s) 374
AspLEI GCGC 1 cut(s) 286
AspS9I GGNCC 2 cut(s) 467, 626
AsuC2I CCSGG 1 cut(s) 803
AsuHPI GGTGA 6 cut(s) 253, 379, 569, 668, 919, 1043
AvaII GGWCC 2 cut(s) 467, 626
BaeGI GKGCMC 1 cut(s) 771
BalI TGGCCA 1 cut(s) 950
BanI GGYRCC 1 cut(s) 798
BbsI GAAGAC 2 cut(s) 84, 1147
BbvCI CCTCAGC 1 cut(s) 790
BbvI GCAGC 5 cut(s) 43, 126, 627, 667, 670
BccI CCATC 5 cut(s) 104, 626, 653, 800, 929
BceAI ACGGC 1 cut(s) 869
BciT130I CCWGG 1 cut(s) 471
BcnI CCSGG 1 cut(s) 803
BcoDI GTCTC 1 cut(s) 750
BfaI CTAG 4 cut(s) 60, 495, 881, 1159
BglII AGATCT 1 cut(s) 153
BisI GCNGC 5 cut(s) 57, 115, 641, 681, 684
BlpI GCTNAGC 1 cut(s) 957
BlsI GCNGC 5 cut(s) 58, 116, 642, 682, 685
BmcAI AGTACT 2 cut(s) 25, 651
Bme1390I CCNGG 2 cut(s) 471, 803
Bme18I GGWCC 2 cut(s) 467, 626
BmgT120I GGNCC 2 cut(s) 467, 626
BmiI GGNNCC 4 cut(s) 628, 800, 830, 866
BmrFI CCNGG 2 cut(s) 471, 803
BmrI ACTGGG 2 cut(s) 834, 985
BmuI ACTGGG 2 cut(s) 834, 985
BpiI GAAGAC 2 cut(s) 84, 1147
BplI GAGNNNNNCTC 2 cut(s) 113, 145
Bpu10I CCTNAGC 1 cut(s) 790
Bpu1102I GCTNAGC 1 cut(s) 957
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 1 cut(s) 803
BsaBI GATNNNNATC 2 cut(s) 165, 443
BsaI GGTCTC 1 cut(s) 750
BsaJI CCNNGG 4 cut(s) 242, 316, 470, 553
BsaWI WCCGGW 1 cut(s) 263
Bsc4I CCNNNNNNNGG 2 cut(s) 343, 795
Bse1I ACTGG 3 cut(s) 48, 829, 991
Bse3DI GCAATG 2 cut(s) 951, 1035
Bse8I GATNNNNATC 2 cut(s) 165, 443
BseBI CCWGG 1 cut(s) 471
BseDI CCNNGG 4 cut(s) 242, 316, 470, 553
BseGI GGATG 1 cut(s) 811
BseJI GATNNNNATC 2 cut(s) 165, 443
BseLI CCNNNNNNNGG 2 cut(s) 343, 795
BseMI GCAATG 2 cut(s) 951, 1035
BseMII CTCAG 4 cut(s) 111, 157, 804, 948
BseNI ACTGG 3 cut(s) 48, 829, 991
BseSI GKGCMC 1 cut(s) 771
BseXI GCAGC 5 cut(s) 43, 126, 627, 667, 670
BseYI CCCAGC 1 cut(s) 542
BsgI GTGCAG 4 cut(s) 521, 684, 727, 1015
Bsh1236I CGCG 1 cut(s) 286
Bsh1285I CGRYCG 1 cut(s) 322
BshFI GGCC 2 cut(s) 325, 950
BshNI GGYRCC 1 cut(s) 798
BsiEI CGRYCG 1 cut(s) 322
BsiSI CCGG 3 cut(s) 264, 803, 1041
BslFI GGGAC 3 cut(s) 612, 862, 869
BslI CCNNNNNNNGG 2 cut(s) 343, 795
BsmAI GTCTC 1 cut(s) 750
BsmFI GGGAC 3 cut(s) 612, 862, 869
BsnI GGCC 2 cut(s) 325, 950
Bso31I GGTCTC 1 cut(s) 750
Bsp1286I GDGCHC 1 cut(s) 771
Bsp143I GATC 6 cut(s) 153, 171, 438, 444, 1021, 1090
Bsp1720I GCTNAGC 1 cut(s) 957
Bsp19I CCATGG 1 cut(s) 242
BspACI CCGC 3 cut(s) 90, 579, 965
BspANI GGCC 2 cut(s) 325, 950
BspCNI CTCAG 4 cut(s) 112, 156, 803, 949
BspFNI CGCG 1 cut(s) 286
BspHI TCATGA 1 cut(s) 441
BspLI GGNNCC 4 cut(s) 628, 800, 830, 866
BspT107I GGYRCC 1 cut(s) 798
BspTNI GGTCTC 1 cut(s) 750
BsrBI CCGCTC 1 cut(s) 90
BsrDI GCAATG 2 cut(s) 951, 1035
BsrI ACTGG 3 cut(s) 48, 829, 991
BssECI CCNNGG 4 cut(s) 242, 316, 470, 553
BssMI GATC 6 cut(s) 153, 171, 438, 444, 1021, 1090
BssNAI GTATAC 1 cut(s) 745
BssT1I CCWWGG 2 cut(s) 242, 553
Bst1107I GTATAC 1 cut(s) 745
Bst2UI CCWGG 1 cut(s) 471
Bst4CI ACNGT 3 cut(s) 28, 278, 671
Bst6I CTCTTC 1 cut(s) 365
BstC8I GCNNGC 3 cut(s) 353, 645, 963
BstDEI CTNAG 6 cut(s) 120, 143, 772, 790, 817, 957
BstDSI CCRYGG 1 cut(s) 242
BstENI CCTNNNNNAGG 1 cut(s) 341
BstF5I GGATG 1 cut(s) 811
BstFNI CGCG 1 cut(s) 286
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 6 cut(s) 156, 174, 441, 447, 1024, 1093
BstMAI GTCTC 1 cut(s) 750
BstMBI GATC 6 cut(s) 153, 171, 438, 444, 1021, 1090
BstMCI CGRYCG 1 cut(s) 322
BstMWI GCNNNNNNNGC 4 cut(s) 292, 576, 914, 962
BstNI CCWGG 1 cut(s) 471
BstSCI CCNGG 2 cut(s) 469, 801
BstSLI GKGCMC 1 cut(s) 771
BstUI CGCG 1 cut(s) 286
BstV1I GCAGC 5 cut(s) 43, 126, 627, 667, 670
BstV2I GAAGAC 2 cut(s) 84, 1147
BstX2I RGATCY 1 cut(s) 153
BstYI RGATCY 1 cut(s) 153
BstZ17I GTATAC 1 cut(s) 745
BsuRI GGCC 2 cut(s) 325, 950
BtgI CCRYGG 1 cut(s) 242
BtsCI GGATG 1 cut(s) 811
BtsIMutI CAGTG 2 cut(s) 55, 998
Cac8I GCNNGC 3 cut(s) 353, 645, 963
CciI TCATGA 1 cut(s) 441
CfoI GCGC 1 cut(s) 286
Cfr13I GGNCC 2 cut(s) 467, 626
Csp6I GTAC 4 cut(s) 24, 274, 650, 1010
CviAII CATG 3 cut(s) 41, 243, 442
CviQI GTAC 4 cut(s) 24, 274, 650, 1010
DdeI CTNAG 6 cut(s) 120, 143, 772, 790, 817, 957
DpnI GATC 6 cut(s) 155, 173, 440, 446, 1023, 1092
DpnII GATC 6 cut(s) 153, 171, 438, 444, 1021, 1090
DraI TTTAAA 2 cut(s) 103, 186
EaeI YGGCCR 1 cut(s) 948
Eam1104I CTCTTC 1 cut(s) 365
EarI CTCTTC 1 cut(s) 365
Eco130I CCWWGG 2 cut(s) 242, 553
Eco31I GGTCTC 1 cut(s) 750
Eco47I GGWCC 2 cut(s) 467, 626
Eco57I CTGAAG 2 cut(s) 1036, 1086
EcoNI CCTNNNNNAGG 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 467
EcoRII CCWGG 1 cut(s) 469
EcoT14I CCWWGG 2 cut(s) 242, 553
ErhI CCWWGG 2 cut(s) 242, 553
FaeI CATG 3 cut(s) 44, 246, 445
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FaqI GGGAC 3 cut(s) 612, 862, 869
FatI CATG 3 cut(s) 40, 242, 441
FauI CCCGC 1 cut(s) 572
FauNDI CATATG 1 cut(s) 1099
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 5 cut(s) 57, 115, 641, 681, 684
FokI GGATG 1 cut(s) 818
Fsp4HI GCNGC 5 cut(s) 57, 115, 641, 681, 684
FspBI CTAG 4 cut(s) 60, 495, 881, 1159
GlaI GCGC 1 cut(s) 285
GluI GCNGC 5 cut(s) 57, 115, 641, 681, 684
GsaI CCCAGC 1 cut(s) 546
HaeIII GGCC 2 cut(s) 325, 950
HapII CCGG 3 cut(s) 264, 803, 1041
HhaI GCGC 1 cut(s) 286
Hin1II CATG 3 cut(s) 44, 246, 445
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HindIII AAGCTT 1 cut(s) 1068
HinfI GANTC 8 cut(s) 44, 122, 128, 134, 608, 813, 875, 1061
HpaII CCGG 3 cut(s) 264, 803, 1041
HphI GGTGA 6 cut(s) 253, 379, 569, 668, 919, 1043
Hpy166II GTNNAC 3 cut(s) 414, 560, 745
Hpy188III TCNNGA 6 cut(s) 145, 332, 442, 495, 629, 1145
Hpy8I GTNNAC 3 cut(s) 414, 560, 745
HpyAV CCTTC 5 cut(s) 203, 337, 358, 886, 1060
HpyCH4III ACNGT 3 cut(s) 28, 278, 671
HpyCH4V TGCA 9 cut(s) 355, 424, 502, 570, 647, 665, 708, 996, 1004
HpyF10VI GCNNNNNNNGC 4 cut(s) 292, 576, 914, 962
HpyF3I CTNAG 6 cut(s) 120, 143, 772, 790, 817, 957
Hsp92II CATG 3 cut(s) 44, 246, 445
HspAI GCGC 1 cut(s) 284
Kzo9I GATC 6 cut(s) 153, 171, 438, 444, 1021, 1090
LmnI GCTCC 1 cut(s) 905
Lsp1109I GCAGC 5 cut(s) 43, 126, 627, 667, 670
MaeI CTAG 4 cut(s) 60, 495, 881, 1159
MaeIII GTNAC 2 cut(s) 457, 1031
MalI GATC 6 cut(s) 155, 173, 440, 446, 1023, 1092
MbiI CCGCTC 1 cut(s) 90
MboI GATC 6 cut(s) 153, 171, 438, 444, 1021, 1090
MboII GAAGA 5 cut(s) 89, 163, 382, 909, 1147
MfeI CAATTG 1 cut(s) 417
MflI RGATCY 1 cut(s) 153
MhlI GDGCHC 1 cut(s) 771
MlsI TGGCCA 1 cut(s) 950
MluCI AATT 4 cut(s) 256, 417, 529, 923
MluNI TGGCCA 1 cut(s) 950
MlyI GAGTC 5 cut(s) 53, 122, 131, 884, 1055
MmeI TCCRAC 1 cut(s) 733
Mox20I TGGCCA 1 cut(s) 950
MroXI GAANNNNTTC 1 cut(s) 374
MscI TGGCCA 1 cut(s) 950
MseI TTAA 5 cut(s) 83, 102, 185, 255, 387
MslI CAYNNNNRTG 2 cut(s) 419, 804
Msp20I TGGCCA 1 cut(s) 950
MspA1I CMGCKG 1 cut(s) 965
MspI CCGG 3 cut(s) 264, 803, 1041
MspR9I CCNGG 2 cut(s) 471, 803
MunI CAATTG 1 cut(s) 417
MvaI CCWGG 1 cut(s) 471
MvnI CGCG 1 cut(s) 286
MwoI GCNNNNNNNGC 4 cut(s) 292, 576, 914, 962
NciI CCSGG 1 cut(s) 803
NcoI CCATGG 1 cut(s) 242
NdeI CATATG 1 cut(s) 1099
NdeII GATC 6 cut(s) 153, 171, 438, 444, 1021, 1090
NlaIII CATG 3 cut(s) 44, 246, 445
NlaIV GGNNCC 4 cut(s) 628, 800, 830, 866
NmuCI GTSAC 1 cut(s) 1031
OliI CACNNNNGTG 1 cut(s) 419
PagI TCATGA 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 374
PfeI GAWTC 3 cut(s) 134, 608, 813
PkrI GCNGC 5 cut(s) 58, 116, 642, 682, 685
PleI GAGTC 5 cut(s) 52, 122, 130, 883, 1055
PpsI GAGTC 5 cut(s) 52, 122, 130, 883, 1055
PpuMI RGGWCCY 1 cut(s) 467
PsiI TTATAA 1 cut(s) 588
Psp5II RGGWCCY 1 cut(s) 467
Psp6I CCWGG 1 cut(s) 469
PspFI CCCAGC 1 cut(s) 542
PspGI CCWGG 1 cut(s) 469
PspN4I GGNNCC 4 cut(s) 628, 800, 830, 866
PspPI GGNCC 2 cut(s) 467, 626
PspPPI RGGWCCY 1 cut(s) 467
PsuI RGATCY 1 cut(s) 153
RsaI GTAC 4 cut(s) 25, 275, 651, 1011
RsaNI GTAC 4 cut(s) 24, 274, 650, 1010
RseI CAYNNNNRTG 2 cut(s) 419, 804
SaqAI TTAA 5 cut(s) 83, 102, 185, 255, 387
SatI GCNGC 5 cut(s) 57, 115, 641, 681, 684
Sau3AI GATC 6 cut(s) 153, 171, 438, 444, 1021, 1090
Sau96I GGNCC 2 cut(s) 467, 626
ScaI AGTACT 2 cut(s) 25, 651
SchI GAGTC 5 cut(s) 53, 122, 131, 884, 1055
ScrFI CCNGG 2 cut(s) 471, 803
SduI GDGCHC 1 cut(s) 771
SinI GGWCC 2 cut(s) 467, 626
SmiMI CAYNNNNRTG 2 cut(s) 419, 804
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
Sse9I AATT 4 cut(s) 256, 417, 529, 923
SsiI CCGC 3 cut(s) 90, 579, 965
SspMI CTAG 4 cut(s) 60, 495, 881, 1159
StyD4I CCNGG 2 cut(s) 469, 801
StyI CCWWGG 2 cut(s) 242, 553
TaaI ACNGT 3 cut(s) 28, 278, 671
TaqI TCGA 3 cut(s) 174, 599, 611
TaqII GACCGA 1 cut(s) 308
TasI AATT 4 cut(s) 256, 417, 529, 923
TatI WGTACW 2 cut(s) 23, 649
TfiI GAWTC 3 cut(s) 134, 608, 813
Tru1I TTAA 5 cut(s) 83, 102, 185, 255, 387
Tru9I TTAA 5 cut(s) 83, 102, 185, 255, 387
TscAI CASTG 2 cut(s) 55, 998
TseFI GTSAC 1 cut(s) 1031
TseI GCWGC 5 cut(s) 56, 114, 640, 680, 683
Tsp45I GTSAC 1 cut(s) 1031
TspDTI ATGAA 1 cut(s) 965
TspRI CASTG 2 cut(s) 55, 998
VpaK11BI GGWCC 2 cut(s) 467, 626
XagI CCTNNNNNAGG 1 cut(s) 341
XapI RAATTY 2 cut(s) 529, 923
XbaI TCTAGA 1 cut(s) 494
XmiI GTMKAC 1 cut(s) 744
XmnI GAANNNNTTC 1 cut(s) 374
XspI CTAG 4 cut(s) 60, 495, 881, 1159
ZrmI AGTACT 2 cut(s) 25, 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.