RLG00000000732

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001698
Physical Location & Seq
Forward (+)
4573221 .. 4578303
5083 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000732

Sequence Viewer

Length: 1152 bp
ATGGAAGTGAATGAGAAGTGTGATGTTTATAGCTTTGGAGTGGTGACATTGGAGACAGTTATGGGAAGACATCCGGGAGATCTTCTCTCATCTCTATCTTCGGGGGCGTTATCATCATCGTCAACTGCATCACCAGCCCATCAATTGCCAGTTGTGGATGTTTTGGACCAACGCATTTCCCCTCCTACACGTGAAGTTGCAGGAGAAGTTGTCTCTGTTGTGAAGGTAGCATTTGCATGCCTGAATTCCAGTCCTAAATATCGTCCAACAATGAAGCAAGTTTCTCAACACCTCTCAACTAAAAGACTGCATTTGTCAGATCCAATATCTATGATAACATGCGATTCTTCCAAAAGAACTCCCTTTTCTACTTCCTACCTAATCAGCTCTGTTCTGGCGTATTTCCGGAACGATGACCTGGAAAGCATAGCAATGCTCAAGCTCCTCCTCCACAACTTCCACCGTCTGAGCCTCCCCAATTCCCCCAAATTCCGATGCGTCTTGCATCAGATTGCCCCCAACTTGGCCATCTCCTTCTCCCTCAGTTCCGTCTTCACCTCTGCCAACCTCATCGCCGTCGCCGCCGCCTCCGGCTCCGGCTTTCTCCACCGCACCGTCATCACTGTCACCGCCGTCCCCAAACTCTTAAAGACAGCCAGTTTGGGCTGTGATCCAACATATAGAATTGAATTTGATGATCTGCTTCCACTGAAAGAGTTTAATTGTCCGTCAAGGCAGGCTGTCGGGGGCCTGAAAGGGCACTTAGACTATTGCTTTTCTTCAGCTTGGCACCCGGATGGACGAATCTTGGCTACTGGGAACCAAGACACTACCTGTAGGCTATGGGACGTAAGGAACCTGTCAGAGTCCCTAGCACTATTGAAGGGAAGAATGGGAGCAATAAGAGCTATAAAATTCACCTCTGATGGTCGTTTTATGGCCATGGCTGAGCCAGCAGACTTCGTTCACATCATTGATACCCAGTCCGGATATGCAAAGGGACAAGAGATTGATCTGTTTGGTGAGATTGCCGGAATATCCTTCAGCCCCGACTCTGAAGCTTTGTTTGTTGGGGTTGCTGATCGCACGTATGGTAGTGTACTGGAGTTCAATAAGAGGCACTATAATCCCTATCAAGAAATAGTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

384

Amino Acids

41.9

Weight (kDa)

7.18

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 240 - 363 5.3e-13 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 240 - 363 9.9e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 241 - 355 5.4e-17 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 242 - 308 1.4e-06 CDC20/Fizzy WD40 domain
WD40 PF00400 249 - 283 2.8e-07 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 258 - 356 1.9e-08 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 260 - 328 6.2e-11 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 789
AccIII TCCGGA 2 cut(s) 405, 986
AciI CCGC 4 cut(s) 582, 585, 610, 630
AclWI GGATC 2 cut(s) 314, 665
AcoI YGGCCR 2 cut(s) 525, 939
AcsI RAATTY 4 cut(s) 244, 488, 689, 914
AcuI CTGAAG 3 cut(s) 765, 1027, 1077
AcvI CACGTG 1 cut(s) 191
AfaI GTAC 1 cut(s) 1101
AfiI CCNNNNNNNGG 1 cut(s) 523
AflIII ACRYGT 1 cut(s) 188
AgsI TTSAA 3 cut(s) 689, 883, 1111
AjnI CCWGG 1 cut(s) 417
AluBI AGCT 6 cut(s) 33, 387, 442, 785, 908, 1061
AluI AGCT 6 cut(s) 33, 387, 442, 785, 908, 1061
Alw26I GTCTC 2 cut(s) 47, 217
AlwI GGATC 2 cut(s) 314, 665
Aor13HI TCCGGA 2 cut(s) 405, 986
AoxI GGCC 3 cut(s) 525, 748, 939
ApoI RAATTY 4 cut(s) 244, 488, 689, 914
ArsI GACNNNNNNTTYG 2 cut(s) 643, 675
AspS9I GGNCC 2 cut(s) 166, 748
AsuC2I CCSGG 2 cut(s) 75, 794
AsuHPI GGTGA 6 cut(s) 55, 123, 547, 619, 910, 1034
AvaII GGWCC 1 cut(s) 166
BaeGI GKGCMC 1 cut(s) 762
BalI TGGCCA 2 cut(s) 527, 941
BanI GGYRCC 1 cut(s) 789
BbrPI CACGTG 1 cut(s) 191
BbsI GAAGAC 3 cut(s) 73, 544, 1138
BccI CCATC 4 cut(s) 147, 536, 791, 920
BceAI ACGGC 2 cut(s) 560, 617
BciT130I CCWGG 1 cut(s) 419
BcnI CCSGG 2 cut(s) 75, 794
BcoDI GTCTC 2 cut(s) 47, 217
BfaI CTAG 2 cut(s) 872, 1150
BfmI CTRYAG 1 cut(s) 835
BglII AGATCT 1 cut(s) 79
BisI GCNGC 2 cut(s) 582, 585
BlpI GCTNAGC 1 cut(s) 948
BlsI GCNGC 2 cut(s) 583, 586
Bme1390I CCNGG 3 cut(s) 75, 419, 794
Bme18I GGWCC 1 cut(s) 166
BmgT120I GGNCC 2 cut(s) 166, 748
BmiI GGNNCC 5 cut(s) 595, 749, 791, 821, 857
BmrFI CCNGG 3 cut(s) 75, 419, 794
BmrI ACTGGG 2 cut(s) 825, 976
BmsI GCATC 3 cut(s) 137, 485, 514
BmuI ACTGGG 2 cut(s) 825, 976
BpiI GAAGAC 3 cut(s) 73, 544, 1138
BplI GAGNNNNNCTC 2 cut(s) 69, 101
BpmI CTGGAG 1 cut(s) 1124
Bpu1102I GCTNAGC 1 cut(s) 948
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 2 cut(s) 75, 794
BsaAI YACGTR 2 cut(s) 191, 1089
BsaJI CCNNGG 1 cut(s) 942
BsaWI WCCGGW 2 cut(s) 405, 986
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 523
Bse1I ACTGG 6 cut(s) 149, 249, 657, 820, 982, 1107
Bse3DI GCAATG 1 cut(s) 438
BseAI TCCGGA 2 cut(s) 405, 986
BseBI CCWGG 1 cut(s) 419
BseDI CCNNGG 1 cut(s) 942
BseGI GGATG 3 cut(s) 70, 163, 802
BseLI CCNNNNNNNGG 1 cut(s) 523
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 3 cut(s) 458, 556, 939
BseNI ACTGG 6 cut(s) 149, 249, 657, 820, 982, 1107
BseRI GAGGAG 2 cut(s) 434, 437
BseSI GKGCMC 1 cut(s) 762
BshFI GGCC 3 cut(s) 527, 750, 941
BshNI GGYRCC 1 cut(s) 789
BsiSI CCGG 7 cut(s) 74, 406, 591, 597, 794, 987, 1032
BslFI GGGAC 4 cut(s) 620, 853, 860, 1014
BslI CCNNNNNNNGG 1 cut(s) 523
BsmAI GTCTC 2 cut(s) 47, 217
BsmFI GGGAC 4 cut(s) 620, 853, 860, 1014
BsnI GGCC 3 cut(s) 527, 750, 941
Bsp1286I GDGCHC 1 cut(s) 762
Bsp13I TCCGGA 2 cut(s) 405, 986
Bsp143I GATC 6 cut(s) 79, 319, 670, 697, 1012, 1081
Bsp1720I GCTNAGC 1 cut(s) 948
Bsp19I CCATGG 1 cut(s) 942
BspACI CCGC 4 cut(s) 582, 585, 610, 630
BspANI GGCC 3 cut(s) 527, 750, 941
BspCNI CTCAG 3 cut(s) 459, 555, 940
BspEI TCCGGA 2 cut(s) 405, 986
BspLI GGNNCC 5 cut(s) 595, 749, 791, 821, 857
BspPI GGATC 2 cut(s) 314, 665
BspT107I GGYRCC 1 cut(s) 789
BsrDI GCAATG 1 cut(s) 438
BsrI ACTGG 6 cut(s) 149, 249, 657, 820, 982, 1107
BssECI CCNNGG 1 cut(s) 942
BssMI GATC 6 cut(s) 79, 319, 670, 697, 1012, 1081
BssT1I CCWWGG 1 cut(s) 942
Bst2UI CCWGG 1 cut(s) 419
Bst4CI ACNGT 4 cut(s) 58, 464, 616, 625
BstBAI YACGTR 2 cut(s) 191, 1089
BstC8I GCNNGC 3 cut(s) 238, 738, 954
BstDEI CTNAG 4 cut(s) 467, 542, 763, 948
BstDSI CCRYGG 1 cut(s) 942
BstF5I GGATG 3 cut(s) 70, 163, 802
BstKTI GATC 6 cut(s) 82, 322, 673, 700, 1015, 1084
BstMAI GTCTC 2 cut(s) 47, 217
BstMBI GATC 6 cut(s) 79, 319, 670, 697, 1012, 1081
BstMWI GCNNNNNNNGC 4 cut(s) 134, 581, 905, 953
BstNI CCWGG 1 cut(s) 419
BstNSI RCATGY 2 cut(s) 240, 342
BstSCI CCNGG 3 cut(s) 73, 417, 792
BstSFI CTRYAG 1 cut(s) 835
BstSLI GKGCMC 1 cut(s) 762
BstV2I GAAGAC 3 cut(s) 73, 544, 1138
BstX2I RGATCY 2 cut(s) 79, 319
BstYI RGATCY 2 cut(s) 79, 319
BsuRI GGCC 3 cut(s) 527, 750, 941
BtgI CCRYGG 1 cut(s) 942
BtgZI GCGATG 1 cut(s) 556
BtsCI GGATG 3 cut(s) 70, 163, 802
BtsIMutI CAGTG 2 cut(s) 621, 707
Cac8I GCNNGC 3 cut(s) 238, 738, 954
Cfr13I GGNCC 2 cut(s) 166, 748
CseI GACGC 1 cut(s) 487
Csp6I GTAC 1 cut(s) 1100
CviAII CATG 3 cut(s) 237, 339, 943
CviQI GTAC 1 cut(s) 1100
DdeI CTNAG 4 cut(s) 467, 542, 763, 948
DpnI GATC 6 cut(s) 81, 321, 672, 699, 1014, 1083
DpnII GATC 6 cut(s) 79, 319, 670, 697, 1012, 1081
EaeI YGGCCR 2 cut(s) 525, 939
Eco130I CCWWGG 1 cut(s) 942
Eco47I GGWCC 1 cut(s) 166
Eco57I CTGAAG 3 cut(s) 765, 1027, 1077
Eco72I CACGTG 1 cut(s) 191
EcoO109I RGGNCCY 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 417
EcoT14I CCWWGG 1 cut(s) 942
ErhI CCWWGG 1 cut(s) 942
FaeI CATG 3 cut(s) 240, 342, 946
FaqI GGGAC 4 cut(s) 620, 853, 860, 1014
FatI CATG 3 cut(s) 236, 338, 942
Fnu4HI GCNGC 2 cut(s) 582, 585
FokI GGATG 3 cut(s) 57, 170, 809
Fsp4HI GCNGC 2 cut(s) 582, 585
FspBI CTAG 2 cut(s) 872, 1150
GluI GCNGC 2 cut(s) 582, 585
GsuI CTGGAG 1 cut(s) 1124
HaeIII GGCC 3 cut(s) 527, 750, 941
HapII CCGG 7 cut(s) 74, 406, 591, 597, 794, 987, 1032
HgaI GACGC 1 cut(s) 487
Hin1II CATG 3 cut(s) 240, 342, 946
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HindIII AAGCTT 1 cut(s) 1059
HinfI GANTC 4 cut(s) 344, 804, 866, 1052
HpaII CCGG 7 cut(s) 74, 406, 591, 597, 794, 987, 1032
HphI GGTGA 6 cut(s) 55, 123, 547, 619, 910, 1034
Hpy166II GTNNAC 3 cut(s) 123, 967, 1100
Hpy188I TCNGA 7 cut(s) 319, 468, 494, 510, 865, 925, 1057
Hpy188III TCNNGA 3 cut(s) 406, 987, 1136
Hpy8I GTNNAC 3 cut(s) 123, 967, 1100
Hpy99I CGWCG 1 cut(s) 581
HpyAV CCTTC 4 cut(s) 217, 544, 877, 1051
HpyCH4III ACNGT 4 cut(s) 58, 464, 616, 625
HpyCH4IV ACGT 3 cut(s) 190, 849, 1088
HpyCH4V TGCA 6 cut(s) 128, 200, 236, 310, 505, 995
HpyF10VI GCNNNNNNNGC 4 cut(s) 134, 581, 905, 953
HpyF3I CTNAG 4 cut(s) 467, 542, 763, 948
HpySE526I ACGT 3 cut(s) 190, 849, 1088
Hsp92II CATG 3 cut(s) 240, 342, 946
Kpn2I TCCGGA 2 cut(s) 405, 986
Kzo9I GATC 6 cut(s) 79, 319, 670, 697, 1012, 1081
LmnI GCTCC 3 cut(s) 447, 599, 896
LweI GCATC 3 cut(s) 137, 485, 514
MaeI CTAG 2 cut(s) 872, 1150
MaeII ACGT 3 cut(s) 190, 849, 1088
MaeIII GTNAC 2 cut(s) 43, 625
MalI GATC 6 cut(s) 81, 321, 672, 699, 1014, 1083
MboI GATC 6 cut(s) 79, 319, 670, 697, 1012, 1081
MboII GAAGA 8 cut(s) 74, 78, 90, 339, 544, 771, 900, 1138
MfeI CAATTG 1 cut(s) 143
MflI RGATCY 2 cut(s) 79, 319
MhlI GDGCHC 1 cut(s) 762
MlsI TGGCCA 2 cut(s) 527, 941
MluCI AATT 8 cut(s) 143, 244, 478, 488, 684, 689, 721, 914
MluNI TGGCCA 2 cut(s) 527, 941
MlyI GAGTC 2 cut(s) 875, 1046
MmeI TCCRAC 2 cut(s) 290, 698
Mox20I TGGCCA 2 cut(s) 527, 941
MroI TCCGGA 2 cut(s) 405, 986
MscI TGGCCA 2 cut(s) 527, 941
MseI TTAA 2 cut(s) 647, 720
MslI CAYNNNNRTG 3 cut(s) 235, 431, 795
Msp20I TGGCCA 2 cut(s) 527, 941
MspI CCGG 7 cut(s) 74, 406, 591, 597, 794, 987, 1032
MspR9I CCNGG 3 cut(s) 75, 419, 794
MunI CAATTG 1 cut(s) 143
MvaI CCWGG 1 cut(s) 419
MwoI GCNNNNNNNGC 4 cut(s) 134, 581, 905, 953
NciI CCSGG 2 cut(s) 75, 794
NcoI CCATGG 1 cut(s) 942
NdeII GATC 6 cut(s) 79, 319, 670, 697, 1012, 1081
NlaIII CATG 3 cut(s) 240, 342, 946
NlaIV GGNNCC 5 cut(s) 595, 749, 791, 821, 857
NmuCI GTSAC 2 cut(s) 43, 625
NspI RCATGY 2 cut(s) 240, 342
PaeI GCATGC 1 cut(s) 240
PfeI GAWTC 2 cut(s) 344, 804
PfoI TCCNGGA 1 cut(s) 73
PkrI GCNGC 2 cut(s) 583, 586
PleI GAGTC 2 cut(s) 874, 1046
PmaCI CACGTG 1 cut(s) 191
PmlI CACGTG 1 cut(s) 191
PpsI GAGTC 2 cut(s) 874, 1046
Ppu21I YACGTR 2 cut(s) 191, 1089
Psp6I CCWGG 1 cut(s) 417
PspCI CACGTG 1 cut(s) 191
PspGI CCWGG 1 cut(s) 417
PspN4I GGNNCC 5 cut(s) 595, 749, 791, 821, 857
PspPI GGNCC 2 cut(s) 166, 748
PsuI RGATCY 2 cut(s) 79, 319
RsaI GTAC 1 cut(s) 1101
RsaNI GTAC 1 cut(s) 1100
RseI CAYNNNNRTG 3 cut(s) 235, 431, 795
SaqAI TTAA 2 cut(s) 647, 720
SatI GCNGC 2 cut(s) 582, 585
Sau3AI GATC 6 cut(s) 79, 319, 670, 697, 1012, 1081
Sau96I GGNCC 2 cut(s) 166, 748
SchI GAGTC 2 cut(s) 875, 1046
ScrFI CCNGG 3 cut(s) 75, 419, 794
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 3 cut(s) 137, 485, 514
SfcI CTRYAG 1 cut(s) 835
SinI GGWCC 1 cut(s) 166
SmiMI CAYNNNNRTG 3 cut(s) 235, 431, 795
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SphI GCATGC 1 cut(s) 240
Sse9I AATT 8 cut(s) 143, 244, 478, 488, 684, 689, 721, 914
SsiI CCGC 4 cut(s) 582, 585, 610, 630
SspMI CTAG 2 cut(s) 872, 1150
StyD4I CCNGG 3 cut(s) 73, 417, 792
StyI CCWWGG 1 cut(s) 942
TaaI ACNGT 4 cut(s) 58, 464, 616, 625
TaiI ACGT 3 cut(s) 193, 852, 1091
TasI AATT 8 cut(s) 143, 244, 478, 488, 684, 689, 721, 914
TatI WGTACW 1 cut(s) 1099
TauI GCSGC 2 cut(s) 584, 587
TfiI GAWTC 2 cut(s) 344, 804
Tru1I TTAA 2 cut(s) 647, 720
Tru9I TTAA 2 cut(s) 647, 720
TscAI CASTG 2 cut(s) 628, 714
TseFI GTSAC 2 cut(s) 43, 625
Tsp45I GTSAC 2 cut(s) 43, 625
TspDTI ATGAA 1 cut(s) 287
TspGWI ACGGA 2 cut(s) 538, 717
TspRI CASTG 2 cut(s) 628, 714
VpaK11BI GGWCC 1 cut(s) 166
XapI RAATTY 4 cut(s) 244, 488, 689, 914
XceI RCATGY 2 cut(s) 240, 342
XspI CTAG 2 cut(s) 872, 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.