Prupe.1G513500_v2.0.a1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001698
Physical Location & Seq
Forward (+)
42257743 .. 42263731
5989 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G513500.1

Sequence Viewer

Length: 1551 bp
ATGGTTGAGCGTTCCTCGCCCTTTTGTCAAACCTCGCTCCAGATTCTTCTTCTCTTTTCACTTTCTCAATCTTTTAGATTCCTCTCATTCTCTCTCACACGCCAACAAAAACGCTTACTTTTTCTGTTTGTTTCTCGGGAAAACTCTACACACTCTGTTTTTCTCACCAGTCCAACTGATTTCTCCCCCAATTCACGGTTGATGGCGCAGTTTCAGAACGACGAGCTGGAGTATGTCGTCGATGACTACTTCGACGTCGCTGAGTTCGAAGACAACGACGCTTTTGCTGAGAACCAGCTCCGTGGGAGCCAAGATGCCGACTCAGACTTTGAGGACGACTTTGATGAGCAGCGCAAGGTGAAGACTGATACTTCAGCTATGGAAGCTAGAAACGGGAAAGACATACAGGGAATTCCATGGGAGAGGCTTAACTTCACCAGAGATAAGTATCGTGAGACACGATTGAAGCAGTACAAGAACTACGAGAGCCTCTCTCGCCCTCGTCAAGACCTCGAAAAGGAGTGCTTGCGAGTAGAGATGGGGAAGACCTTCTATGACTTTCACTACAATACAAGGATTGTCAAGTCTACAATTGTCCATTTCCAGCTGAGGAATTTGGTATGGGCCACATCAAAGCATGATGTATACCTTATGCAGAACTTTTCAGTAATGCACTGGTCCTCTTTGCTCAGGAGAGGGAAAGAAGTACTCAATGTATCAAAACCAATTGTGCCTACTGAGAAACGACCTGGGTTGTTGGCTCAGCCACTCTCTAGGGTGCAGATAAGCACTATGGCTGTTAAGGAAAACTTATTGGTTGTTGGGGGCTTCCAAGGCGAGCTCATCTGTAAGCACATCAATCAACCTGGAGTTGCTTTCTGCTCAAAAGTAACAGCAGATGACAATGCCATCACAAATGCAGTGGACATTTTCCAAAGCCCTACTGGGTCAATGAGGGTAATGGCTGCAAACAACGATGCTCAAGTCAGAGTTTTTGATGTCCAAAATTTTTCTTGCCTAAATCAATTCTCCTTTGATTGGTCTGTAAATAACACATCTGTTAGTCCAGACGGTAAGCTGGTTGCAGTTCTTGGTGATAGTGCAGACTGCTTGATTGCTGATGCCCAGTCTGGAAAAGCCATTGGAAACCTCAAAGGACACTTAGACTATTCCTTTTCCTCTGCGTGGCACCCGAACGGACAAATTTTGGCTACTGGGAATCAAGACACTACTTGCAGGTTATGGGACATAAGGAACCTCTCAGAGTCCCTCACTGTGTTGAAGGGAAGGATGGGAGCAATAAGAGCCATTAAGTTCAGCTCCGATGGCCGGTTCATGGCCATGGCTGAGCCAGCAGACTTTGTTCACATCATGGATACCCAGTCCGGGTACATCAAGGGACAAGAGATTGATCTGTTTGGTGAGATTGCCGGGATATCCTTTAGCCCTGACACTGAAGCTCTGTTTGTTGGGATCGCCGATCGCACTTATGGTAGCGTATTAGAGTTCAACAAGAGGCATCACAACCAATATCTAGAAGCAGTGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

517

Amino Acids

58.54

Weight (kDa)

5.65

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 258
Acc36I ACCTGC 1 cut(s) 1227
AccB1I GGYRCC 1 cut(s) 1188
AccI GTMKAC 2 cut(s) 587, 645
AclWI GGATC 1 cut(s) 1481
AcoI YGGCCR 2 cut(s) 1327, 1338
AcsI RAATTY 4 cut(s) 411, 613, 1006, 1203
AcuI CTGAAG 2 cut(s) 357, 1476
AcyI GRCGYC 1 cut(s) 255
AfaI GTAC 3 cut(s) 473, 708, 1391
AfiI CCNNNNNNNGG 7 cut(s) 195, 517, 1038, 1185, 1329, 1336, 1386
AgsI TTSAA 3 cut(s) 466, 1282, 1510
AjnI CCWGG 2 cut(s) 748, 865
AluBI AGCT 9 cut(s) 226, 298, 377, 386, 607, 841, 1078, 1320, 1460
AluI AGCT 9 cut(s) 226, 298, 377, 386, 607, 841, 1078, 1320, 1460
Alw21I GWGCWC 1 cut(s) 843
Alw26I GTCTC 1 cut(s) 449
AlwI GGATC 1 cut(s) 1481
Ama87I CYCGRG 1 cut(s) 135
AoxI GGCC 3 cut(s) 624, 1327, 1338
ApeKI GCWGC 2 cut(s) 349, 965
ApoI RAATTY 4 cut(s) 411, 613, 1006, 1203
ArsI GACNNNNNNTTYG 2 cut(s) 311, 343
Asp700I GAANNNNTTC 1 cut(s) 548
AspLEI GCGC 2 cut(s) 208, 354
AspS9I GGNCC 2 cut(s) 624, 678
AsuC2I CCSGG 2 cut(s) 1387, 1432
AsuHPI GGTGA 5 cut(s) 157, 370, 427, 1106, 1433
AsuII TTCGAA 1 cut(s) 267
AvaI CYCGRG 1 cut(s) 135
AvaII GGWCC 1 cut(s) 678
BalI TGGCCA 1 cut(s) 1340
BanI GGYRCC 1 cut(s) 1188
BanII GRGCYC 1 cut(s) 843
BbsI GAAGAC 3 cut(s) 276, 368, 551
Bbv12I GWGCWC 1 cut(s) 843
BbvCI CCTCAGC 1 cut(s) 608
BbvI GCAGC 2 cut(s) 361, 952
BccI CCATC 5 cut(s) 196, 532, 917, 1285, 1319
BcgI CGANNNNNNTGC 2 cut(s) 266, 300
BciT130I CCWGG 2 cut(s) 750, 867
BciVI GTATCC 1 cut(s) 1369
BcnI CCSGG 2 cut(s) 1387, 1432
BcoDI GTCTC 1 cut(s) 449
BfaI CTAG 4 cut(s) 387, 774, 1535, 1549
BfuAI ACCTGC 1 cut(s) 1227
BfuI GTATCC 1 cut(s) 1369
BisI GCNGC 2 cut(s) 350, 966
BlpI GCTNAGC 2 cut(s) 762, 1347
BlsI GCNGC 2 cut(s) 351, 967
BmcAI AGTACT 1 cut(s) 708
Bme1390I CCNGG 4 cut(s) 750, 867, 1387, 1432
Bme18I GGWCC 1 cut(s) 678
BmeT110I CYCGRG 1 cut(s) 135
BmgT120I GGNCC 2 cut(s) 624, 678
BmiI GGNNCC 3 cut(s) 308, 1190, 1256
BmrFI CCNGG 4 cut(s) 750, 867, 1387, 1432
BmrI ACTGGG 4 cut(s) 954, 1120, 1224, 1375
BmsI GCATC 4 cut(s) 304, 967, 1111, 1528
BmuI ACTGGG 4 cut(s) 954, 1120, 1224, 1375
BpiI GAAGAC 3 cut(s) 276, 368, 551
BplI GAGNNNNNCTC 5 cut(s) 31, 476, 508, 478, 510
BpmI CTGGAG 3 cut(s) 23, 248, 888
Bpu10I CCTNAGC 2 cut(s) 608, 689
Bpu1102I GCTNAGC 2 cut(s) 762, 1347
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 1 cut(s) 966
BpuMI CCSGG 2 cut(s) 1387, 1432
BsaBI GATNNNNATC 1 cut(s) 447
BsaHI GRCGYC 1 cut(s) 255
BsaJI CCNNGG 5 cut(s) 301, 416, 749, 832, 1341
Bsc4I CCNNNNNNNGG 7 cut(s) 195, 517, 1038, 1185, 1329, 1336, 1386
Bse118I RCCGGY 1 cut(s) 1329
Bse1I ACTGG 6 cut(s) 168, 680, 949, 1126, 1219, 1381
Bse8I GATNNNNATC 1 cut(s) 447
BseBI CCWGG 2 cut(s) 750, 867
BseDI CCNNGG 5 cut(s) 301, 416, 749, 832, 1341
BseGI GGATG 1 cut(s) 1296
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 7 cut(s) 195, 517, 1038, 1185, 1329, 1336, 1386
BseMII CTCAG 9 cut(s) 252, 279, 336, 599, 703, 729, 776, 1275, 1338
BseNI ACTGG 6 cut(s) 168, 680, 949, 1126, 1219, 1381
BseXI GCAGC 2 cut(s) 361, 952
BsgI GTGCAG 2 cut(s) 800, 1122
Bsh1285I CGRYCG 1 cut(s) 1483
BshFI GGCC 3 cut(s) 626, 1329, 1340
BshNI GGYRCC 1 cut(s) 1188
BsiEI CGRYCG 1 cut(s) 1483
BsiHKAI GWGCWC 1 cut(s) 843
BsiHKCI CYCGRG 1 cut(s) 135
BsiSI CCGG 3 cut(s) 1330, 1386, 1431
BslFI GGGAC 3 cut(s) 1252, 1259, 1413
BslI CCNNNNNNNGG 7 cut(s) 195, 517, 1038, 1185, 1329, 1336, 1386
BsmAI GTCTC 1 cut(s) 449
BsmFI GGGAC 3 cut(s) 1252, 1259, 1413
BsnI GGCC 3 cut(s) 626, 1329, 1340
BsoBI CYCGRG 1 cut(s) 135
Bsp119I TTCGAA 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 843
Bsp143I GATC 3 cut(s) 1411, 1473, 1480
Bsp1720I GCTNAGC 2 cut(s) 762, 1347
Bsp19I CCATGG 2 cut(s) 416, 1341
BspANI GGCC 3 cut(s) 626, 1329, 1340
BspCNI CTCAG 9 cut(s) 253, 280, 335, 600, 702, 730, 775, 1274, 1339
BspLI GGNNCC 3 cut(s) 308, 1190, 1256
BspMI ACCTGC 1 cut(s) 1227
BspPI GGATC 1 cut(s) 1481
BspT104I TTCGAA 1 cut(s) 267
BspT107I GGYRCC 1 cut(s) 1188
BsrFI RCCGGY 1 cut(s) 1329
BsrI ACTGG 6 cut(s) 168, 680, 949, 1126, 1219, 1381
BssAI RCCGGY 1 cut(s) 1329
BssECI CCNNGG 5 cut(s) 301, 416, 749, 832, 1341
BssMI GATC 3 cut(s) 1411, 1473, 1480
BssNAI GTATAC 1 cut(s) 646
BssNI GRCGYC 1 cut(s) 255
BssT1I CCWWGG 3 cut(s) 416, 832, 1341
Bst1107I GTATAC 1 cut(s) 646
Bst2UI CCWGG 2 cut(s) 750, 867
Bst4CI ACNGT 3 cut(s) 198, 1073, 1276
BstACI GRCGYC 1 cut(s) 255
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 3 cut(s) 527, 839, 1353
BstDSI CCRYGG 3 cut(s) 301, 416, 1341
BstENI CCTNNNNNAGG 1 cut(s) 515
BstF5I GGATG 1 cut(s) 1296
BstHHI GCGC 2 cut(s) 208, 354
BstKTI GATC 3 cut(s) 1414, 1476, 1483
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 3 cut(s) 1411, 1473, 1480
BstMCI CGRYCG 1 cut(s) 1483
BstMWI GCNNNNNNNGC 7 cut(s) 16, 383, 495, 834, 1304, 1326, 1352
BstNI CCWGG 2 cut(s) 750, 867
BstSCI CCNGG 4 cut(s) 748, 865, 1385, 1430
BstV1I GCAGC 2 cut(s) 361, 952
BstV2I GAAGAC 3 cut(s) 276, 368, 551
BstXI CCANNNNNNTGG 1 cut(s) 302
BstZ17I GTATAC 1 cut(s) 646
BsuI GTATCC 1 cut(s) 1369
BsuRI GGCC 3 cut(s) 626, 1329, 1340
BtgI CCRYGG 3 cut(s) 301, 416, 1341
BtsCI GGATG 1 cut(s) 1296
BtsI GCAGTG 2 cut(s) 927, 1548
BtsIMutI CAGTG 5 cut(s) 673, 927, 1272, 1452, 1548
BveI ACCTGC 1 cut(s) 1227
Cac8I GCNNGC 3 cut(s) 527, 839, 1353
CfoI GCGC 2 cut(s) 208, 354
Cfr10I RCCGGY 1 cut(s) 1329
Cfr13I GGNCC 2 cut(s) 624, 678
CseI GACGC 1 cut(s) 287
Csp6I GTAC 3 cut(s) 472, 707, 1390
CspCI CAANNNNNGTGG 2 cut(s) 903, 938
CviAII CATG 5 cut(s) 417, 638, 1336, 1342, 1372
CviQI GTAC 3 cut(s) 472, 707, 1390
DpnI GATC 3 cut(s) 1413, 1475, 1482
DpnII GATC 3 cut(s) 1411, 1473, 1480
EaeI YGGCCR 2 cut(s) 1327, 1338
Ecl136II GAGCTC 1 cut(s) 841
Eco130I CCWWGG 3 cut(s) 416, 832, 1341
Eco24I GRGCYC 1 cut(s) 843
Eco32I GATATC 1 cut(s) 1437
Eco47I GGWCC 1 cut(s) 678
Eco53kI GAGCTC 1 cut(s) 841
Eco57I CTGAAG 2 cut(s) 357, 1476
Eco88I CYCGRG 1 cut(s) 135
EcoICRI GAGCTC 1 cut(s) 841
EcoNI CCTNNNNNAGG 1 cut(s) 515
EcoRI GAATTC 1 cut(s) 411
EcoRII CCWGG 2 cut(s) 748, 865
EcoRV GATATC 1 cut(s) 1437
EcoT14I CCWWGG 3 cut(s) 416, 832, 1341
EcoT38I GRGCYC 1 cut(s) 843
ErhI CCWWGG 3 cut(s) 416, 832, 1341
FaeI CATG 5 cut(s) 420, 641, 1339, 1345, 1375
FalI AAGNNNNNCTT 4 cut(s) 509, 541, 794, 826
FaqI GGGAC 3 cut(s) 1252, 1259, 1413
FatI CATG 5 cut(s) 416, 637, 1335, 1341, 1371
FblI GTMKAC 2 cut(s) 587, 645
Fnu4HI GCNGC 2 cut(s) 350, 966
FokI GGATG 1 cut(s) 1303
FriOI GRGCYC 1 cut(s) 843
Fsp4HI GCNGC 2 cut(s) 350, 966
FspBI CTAG 4 cut(s) 387, 774, 1535, 1549
GlaI GCGC 2 cut(s) 207, 353
GluI GCNGC 2 cut(s) 350, 966
GsuI CTGGAG 3 cut(s) 23, 248, 888
HaeIII GGCC 3 cut(s) 626, 1329, 1340
HapII CCGG 3 cut(s) 1330, 1386, 1431
HgaI GACGC 1 cut(s) 287
HhaI GCGC 2 cut(s) 208, 354
Hin1I GRCGYC 1 cut(s) 255
Hin1II CATG 5 cut(s) 420, 641, 1339, 1345, 1375
Hin6I GCGC 2 cut(s) 206, 352
HinP1I GCGC 2 cut(s) 206, 352
HinfI GANTC 5 cut(s) 43, 78, 320, 1219, 1265
HpaII CCGG 3 cut(s) 1330, 1386, 1431
HphI GGTGA 5 cut(s) 157, 370, 427, 1106, 1433
Hpy166II GTNNAC 4 cut(s) 588, 646, 925, 1366
Hpy188I TCNGA 5 cut(s) 216, 325, 989, 1264, 1324
Hpy188III TCNNGA 9 cut(s) 40, 137, 452, 506, 691, 1067, 1131, 1223, 1535
Hpy8I GTNNAC 4 cut(s) 588, 646, 925, 1366
Hpy99I CGWCG 5 cut(s) 224, 242, 257, 260, 281
HpyAV CCTTC 3 cut(s) 559, 1276, 1281
HpyCH4III ACNGT 3 cut(s) 198, 1073, 1276
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 8 cut(s) 655, 673, 781, 920, 968, 1085, 1103, 1236
HpyF10VI GCNNNNNNNGC 7 cut(s) 16, 383, 495, 834, 1304, 1326, 1352
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 1 cut(s) 255
Hsp92II CATG 5 cut(s) 420, 641, 1339, 1345, 1375
HspAI GCGC 2 cut(s) 206, 352
Kzo9I GATC 3 cut(s) 1411, 1473, 1480
LmnI GCTCC 5 cut(s) 42, 303, 306, 1295, 1325
Lsp1109I GCAGC 2 cut(s) 361, 952
LweI GCATC 4 cut(s) 304, 967, 1111, 1528
MaeI CTAG 4 cut(s) 387, 774, 1535, 1549
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 889
MalI GATC 3 cut(s) 1413, 1475, 1482
MboI GATC 3 cut(s) 1411, 1473, 1480
MboII GAAGA 5 cut(s) 38, 41, 281, 373, 556
MfeI CAATTG 2 cut(s) 591, 726
MhlI GDGCHC 1 cut(s) 843
MlsI TGGCCA 1 cut(s) 1340
MluCI AATT 8 cut(s) 190, 411, 591, 613, 726, 1006, 1025, 1203
MluNI TGGCCA 1 cut(s) 1340
MlyI GAGTC 2 cut(s) 314, 1274
MmeI TCCRAC 1 cut(s) 197
Mox20I TGGCCA 1 cut(s) 1340
MroXI GAANNNNTTC 1 cut(s) 548
MscI TGGCCA 1 cut(s) 1340
MseI TTAA 3 cut(s) 429, 801, 1311
MslI CAYNNNNRTG 1 cut(s) 1340
Msp20I TGGCCA 1 cut(s) 1340
MspA1I CMGCKG 1 cut(s) 607
MspI CCGG 3 cut(s) 1330, 1386, 1431
MspR9I CCNGG 4 cut(s) 750, 867, 1387, 1432
MunI CAATTG 2 cut(s) 591, 726
MvaI CCWGG 2 cut(s) 750, 867
MwoI GCNNNNNNNGC 7 cut(s) 16, 383, 495, 834, 1304, 1326, 1352
NciI CCSGG 2 cut(s) 1387, 1432
NcoI CCATGG 2 cut(s) 416, 1341
NdeII GATC 3 cut(s) 1411, 1473, 1480
NlaIII CATG 5 cut(s) 420, 641, 1339, 1345, 1375
NlaIV GGNNCC 3 cut(s) 308, 1190, 1256
NspV TTCGAA 1 cut(s) 267
PcsI WCGNNNNNNNCGW 3 cut(s) 264, 273, 457
PdmI GAANNNNTTC 1 cut(s) 548
PfeI GAWTC 3 cut(s) 43, 78, 1219
PkrI GCNGC 2 cut(s) 351, 967
Ple19I CGATCG 1 cut(s) 1483
PleI GAGTC 2 cut(s) 314, 1273
PpsI GAGTC 2 cut(s) 314, 1273
Psp124BI GAGCTC 1 cut(s) 843
Psp6I CCWGG 2 cut(s) 748, 865
PspGI CCWGG 2 cut(s) 748, 865
PspN4I GGNNCC 3 cut(s) 308, 1190, 1256
PspPI GGNCC 2 cut(s) 624, 678
PvuI CGATCG 1 cut(s) 1483
PvuII CAGCTG 1 cut(s) 607
RsaI GTAC 3 cut(s) 473, 708, 1391
RsaNI GTAC 3 cut(s) 472, 707, 1390
RseI CAYNNNNRTG 1 cut(s) 1340
SacI GAGCTC 1 cut(s) 843
SaqAI TTAA 3 cut(s) 429, 801, 1311
SatI GCNGC 2 cut(s) 350, 966
Sau3AI GATC 3 cut(s) 1411, 1473, 1480
Sau96I GGNCC 2 cut(s) 624, 678
ScaI AGTACT 1 cut(s) 708
SchI GAGTC 2 cut(s) 314, 1274
ScrFI CCNGG 4 cut(s) 750, 867, 1387, 1432
SduI GDGCHC 1 cut(s) 843
SfaNI GCATC 4 cut(s) 304, 967, 1111, 1528
SfuI TTCGAA 1 cut(s) 267
SinI GGWCC 1 cut(s) 678
SmiMI CAYNNNNRTG 1 cut(s) 1340
SmlI CTYRAG 1 cut(s) 981
SmoI CTYRAG 1 cut(s) 981
Sse9I AATT 8 cut(s) 190, 411, 591, 613, 726, 1006, 1025, 1203
SspMI CTAG 4 cut(s) 387, 774, 1535, 1549
SstI GAGCTC 1 cut(s) 843
StyD4I CCNGG 4 cut(s) 748, 865, 1385, 1430
StyI CCWWGG 3 cut(s) 416, 832, 1341
TaaI ACNGT 3 cut(s) 198, 1073, 1276
TaiI ACGT 1 cut(s) 258
TaqI TCGA 4 cut(s) 240, 252, 267, 513
TasI AATT 8 cut(s) 190, 411, 591, 613, 726, 1006, 1025, 1203
TatI WGTACW 2 cut(s) 471, 706
TfiI GAWTC 3 cut(s) 43, 78, 1219
Tru1I TTAA 3 cut(s) 429, 801, 1311
Tru9I TTAA 3 cut(s) 429, 801, 1311
TscAI CASTG 5 cut(s) 680, 927, 1279, 1459, 1548
TseI GCWGC 2 cut(s) 349, 965
TspDTI ATGAA 1 cut(s) 1324
TspGWI ACGGA 2 cut(s) 290, 1212
TspRI CASTG 5 cut(s) 680, 927, 1279, 1459, 1548
VpaK11BI GGWCC 1 cut(s) 678
XagI CCTNNNNNAGG 1 cut(s) 515
XapI RAATTY 4 cut(s) 411, 613, 1006, 1203
XbaI TCTAGA 1 cut(s) 1534
XcmI CCANNNNNNNNNTGG 1 cut(s) 941
XmiI GTMKAC 2 cut(s) 587, 645
XmnI GAANNNNTTC 1 cut(s) 548
XspI CTAG 4 cut(s) 387, 774, 1535, 1549
ZraI GACGTC 1 cut(s) 256
ZrmI AGTACT 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.