AT1G68390

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001447
Physical Location & Seq
Forward (+)
25642534 .. 25645607
3074 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G68390.1

Sequence Viewer

Length: 1227 bp
ATGAAGAACCAAAAGGATCAAAACTCATCATCATTGTCTTCTTCATCACCATCTCTAACAACAAAGCTTCTCAATGCTCAATACCATCACTTCCTCAATCTCCTTTCGTATTCCCTTATCCTCTGTTGCGGTATCATCATCGGCATCCTTCTCCATTCCTCTCTACAAAACTTCTCTTCAAATTCTTCACTTAGCATCCAACGCATATCTCAGCTATTTATAGTCTCTTCTCTTCCTCCTTCTCCTCCTCCTCCTCCTCCTCCTTCTCCTCCTTCTGAGCCCGAGCAAAATGGGCTCAAAAGCTTTATTGAGCCACCGGAGAAGCTTATGCACGACATGGAGGACGAAGAGCTTCTCTGGAGAGCTTCAATGGCGCCTAAGATCAAGAATTACCCTTTTCCTCGAACGCCAAAGGTTGCTTTCATGTTCATGACAAAGGGTCATTTACCTCTAGCTCGTCTATGGGAGAGATTCTTTCGTGGGCATGAAGGTCTTTTTACTATCTACGTTCACTCTTATCCTTCTTATAACCAGTCCGATCCCGAAGATTCCGTCTTCCGTGGCCGCCACATTCCAAGCAAGAGAGTGGATTGGGGATATGTGAACATGGTGGAAGCAGAACAAAGATTACTAGCAAATGCTTTATTAGACATATCAAACGAACGATTCGTACTTCTCTCCGAATCATGCATCCCTCTTTTCAACTTCACCACTGTTTACTCTTACCTTATTAACTCAACTCAAACTCACGTTGAGTCCTACGACCAACTTGGTGGCGTTGGACGCGGCCGATACAGCCCTCTAATGCAGCCACATGTCCAGCTACGCCACTGGCGCAAAGGCTCCCAATGGATCGAAGTGGACCGTGCCATGGCTCTTGAGATTATCTCCGATAGAATCTACTGGCCTCTCTTTTATAGTTATTGCCACCATGGTTGCTACGCCGACGAGCACTATATTCCTACACTCCTCAACATCAAATCAAGCCTGAAACGGCGCAACTCCAACCGGACTCTCACGTGGGTCGACTGGTCGAAAGGTGGCCCGCACCCTAACCGGTTTATTAGACACGAGGTGACTGCGGAGTTCATGGAGAACCTAAGGAGTGGTGGTGAGTGTCTCTACAATGGAGAAGAGACGAATATTTGTTATTTATTTGCTCGCAAGTTCTTGCCTACTGCTCTCGACCGGTTGCTCAGGCTTTCACGTACTGTTTTACATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

408

Amino Acids

47.12

Weight (kDa)

8.72

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 139 - 368 1.6e-82 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 528
AccB1I GGYRCC 1 cut(s) 373
AccI GTMKAC 1 cut(s) 1026
AccII CGCG 1 cut(s) 786
AciI CCGC 5 cut(s) 129, 565, 786, 1046, 1082
AclWI GGATC 3 cut(s) 24, 533, 860
AcoI YGGCCR 2 cut(s) 562, 787
AcsI RAATTY 1 cut(s) 181
AcvI CACGTG 1 cut(s) 1020
AcyI GRCGYC 1 cut(s) 374
AdeI CACNNNGTG 1 cut(s) 1075
AfaI GTAC 2 cut(s) 672, 1210
AfiI CCNNNNNNNGG 1 cut(s) 871
AflIII ACRYGT 1 cut(s) 814
AgeI ACCGGT 2 cut(s) 1056, 1188
AgsI TTSAA 3 cut(s) 180, 369, 703
AhdI GACNNNNNGTC 1 cut(s) 438
AluBI AGCT 8 cut(s) 67, 214, 303, 325, 352, 365, 455, 823
AluI AGCT 8 cut(s) 67, 214, 303, 325, 352, 365, 455, 823
Alw21I GWGCWC 1 cut(s) 954
Alw26I GTCTC 3 cut(s) 229, 1124, 1130
AlwI GGATC 3 cut(s) 24, 533, 860
Ama87I CYCGRG 1 cut(s) 281
AoxI GGCC 4 cut(s) 562, 787, 905, 1042
ApeKI GCWGC 1 cut(s) 808
ApoI RAATTY 1 cut(s) 181
ArsI GACNNNNNNTTYG 2 cut(s) 537, 569
AsiGI ACCGGT 2 cut(s) 1056, 1188
Asp700I GAANNNNTTC 1 cut(s) 351
AspLEI GCGC 3 cut(s) 376, 837, 999
AspS9I GGNCC 2 cut(s) 862, 1043
AsuHPI GGTGA 4 cut(s) 39, 700, 1087, 1124
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 1 cut(s) 862
AxyI CCTNAGG 1 cut(s) 1100
BanI GGYRCC 1 cut(s) 373
BanII GRGCYC 2 cut(s) 282, 297
BauI CACGAG 1 cut(s) 1070
BbrPI CACGTG 1 cut(s) 1020
BbsI GAAGAC 2 cut(s) 30, 547
Bbv12I GWGCWC 1 cut(s) 954
BbvI GCAGC 1 cut(s) 820
BccI CCATC 2 cut(s) 58, 93
BceAI ACGGC 1 cut(s) 1010
BcgI CGANNNNNNTGC 2 cut(s) 1061, 1095
BcoDI GTCTC 3 cut(s) 229, 1124, 1130
BfaI CTAG 2 cut(s) 452, 632
BfoI RGCGCY 1 cut(s) 377
BisI GCNGC 3 cut(s) 565, 787, 809
BlsI GCNGC 3 cut(s) 566, 788, 810
Bme18I GGWCC 1 cut(s) 862
BmeRI GACNNNNNGTC 1 cut(s) 438
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 2 cut(s) 862, 1043
BmiI GGNNCC 2 cut(s) 375, 844
BmsI GCATC 3 cut(s) 153, 204, 699
BpiI GAAGAC 2 cut(s) 30, 547
BplI GAGNNNNNCTC 2 cut(s) 872, 904
BpmI CTGGAG 1 cut(s) 379
Bpu10I CCTNAGC 1 cut(s) 1196
BpuEI CTTGAG 1 cut(s) 899
BsaAI YACGTR 2 cut(s) 1020, 1208
BsaHI GRCGYC 1 cut(s) 374
BsaJI CCNNGG 3 cut(s) 559, 870, 931
BsaWI WCCGGW 4 cut(s) 316, 1008, 1056, 1188
BsaXI ACNNNNNCTCC 2 cut(s) 1096, 1126
Bsc4I CCNNNNNNNGG 1 cut(s) 871
Bse118I RCCGGY 2 cut(s) 1056, 1188
Bse1I ACTGG 4 cut(s) 532, 836, 908, 1034
Bse21I CCTNAGG 1 cut(s) 1100
BseDI CCNNGG 3 cut(s) 559, 870, 931
BseGI GGATG 3 cut(s) 144, 195, 690
BseLI CCNNNNNNNGG 1 cut(s) 871
BseMII CTCAG 3 cut(s) 224, 267, 1210
BseNI ACTGG 4 cut(s) 532, 836, 908, 1034
BseRI GAGGAG 8 cut(s) 234, 237, 240, 243, 246, 249, 258, 959
BseX3I CGGCCG 1 cut(s) 787
BseXI GCAGC 1 cut(s) 820
Bsh1236I CGCG 1 cut(s) 786
Bsh1285I CGRYCG 2 cut(s) 790, 1189
BshFI GGCC 4 cut(s) 564, 789, 907, 1044
BshNI GGYRCC 1 cut(s) 373
BshTI ACCGGT 2 cut(s) 1056, 1188
BsiEI CGRYCG 2 cut(s) 790, 1189
BsiHKAI GWGCWC 1 cut(s) 954
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 4 cut(s) 317, 1009, 1057, 1189
BslI CCNNNNNNNGG 1 cut(s) 871
BsmAI GTCTC 3 cut(s) 229, 1124, 1130
BsmBI CGTCTC 1 cut(s) 1130
BsnI GGCC 4 cut(s) 564, 789, 907, 1044
BsoBI CYCGRG 1 cut(s) 281
Bsp1286I GDGCHC 3 cut(s) 282, 297, 954
Bsp143I GATC 4 cut(s) 16, 381, 538, 852
Bsp19I CCATGG 2 cut(s) 870, 931
BspACI CCGC 5 cut(s) 129, 565, 786, 1046, 1082
BspANI GGCC 4 cut(s) 564, 789, 907, 1044
BspCNI CTCAG 3 cut(s) 223, 268, 1209
BspFNI CGCG 1 cut(s) 786
BspHI TCATGA 1 cut(s) 429
BspLI GGNNCC 2 cut(s) 375, 844
BspPI GGATC 3 cut(s) 24, 533, 860
BspQI GCTCTTC 1 cut(s) 342
BspT107I GGYRCC 1 cut(s) 373
BsrFI RCCGGY 2 cut(s) 1056, 1188
BsrI ACTGG 4 cut(s) 532, 836, 908, 1034
BssAI RCCGGY 2 cut(s) 1056, 1188
BssECI CCNNGG 3 cut(s) 559, 870, 931
BssMI GATC 4 cut(s) 16, 381, 538, 852
BssNI GRCGYC 1 cut(s) 374
BssSI CACGAG 1 cut(s) 1070
BssT1I CCWWGG 2 cut(s) 870, 931
Bst2BI CACGAG 1 cut(s) 1070
Bst4CI ACNGT 3 cut(s) 715, 866, 1213
Bst6I CTCTTC 5 cut(s) 181, 232, 237, 342, 1128
BstACI GRCGYC 1 cut(s) 374
BstBAI YACGTR 2 cut(s) 1020, 1208
BstC8I GCNNGC 2 cut(s) 1046, 1162
BstDEI CTNAG 6 cut(s) 191, 210, 276, 378, 1100, 1196
BstDSI CCRYGG 3 cut(s) 559, 870, 931
BstF5I GGATG 3 cut(s) 144, 195, 690
BstFNI CGCG 1 cut(s) 786
BstH2I RGCGCY 1 cut(s) 377
BstHHI GCGC 3 cut(s) 376, 837, 999
BstKTI GATC 4 cut(s) 19, 384, 541, 855
BstMAI GTCTC 3 cut(s) 229, 1124, 1130
BstMBI GATC 4 cut(s) 16, 381, 538, 852
BstMCI CGRYCG 2 cut(s) 790, 1189
BstMWI GCNNNNNNNGC 6 cut(s) 201, 292, 371, 783, 795, 834
BstNSI RCATGY 1 cut(s) 818
BstUI CGCG 1 cut(s) 786
BstV1I GCAGC 1 cut(s) 820
BstV2I GAAGAC 2 cut(s) 30, 547
BstXI CCANNNNNNTGG 1 cut(s) 773
BstZI CGGCCG 1 cut(s) 787
Bsu36I CCTNAGG 1 cut(s) 1100
BsuRI GGCC 4 cut(s) 564, 789, 907, 1044
BtgI CCRYGG 3 cut(s) 559, 870, 931
BtsCI GGATG 3 cut(s) 144, 195, 690
BtsIMutI CAGTG 2 cut(s) 711, 829
Cac8I GCNNGC 2 cut(s) 1046, 1162
CciI TCATGA 1 cut(s) 429
CfoI GCGC 3 cut(s) 376, 837, 999
Cfr10I RCCGGY 2 cut(s) 1056, 1188
Cfr13I GGNCC 2 cut(s) 862, 1043
CseI GACGC 1 cut(s) 792
Csp6I GTAC 2 cut(s) 671, 1209
CspAI ACCGGT 2 cut(s) 1056, 1188
CspCI CAANNNNNGTGG 2 cut(s) 917, 952
CviQI GTAC 2 cut(s) 671, 1209
DdeI CTNAG 6 cut(s) 191, 210, 276, 378, 1100, 1196
DinI GGCGCC 1 cut(s) 375
DpnI GATC 4 cut(s) 18, 383, 540, 854
DpnII GATC 4 cut(s) 16, 381, 538, 852
DraIII CACNNNGTG 1 cut(s) 1075
DriI GACNNNNNGTC 1 cut(s) 438
EaeI YGGCCR 2 cut(s) 562, 787
EagI CGGCCG 1 cut(s) 787
Eam1104I CTCTTC 5 cut(s) 181, 232, 237, 342, 1128
Eam1105I GACNNNNNGTC 1 cut(s) 438
EarI CTCTTC 5 cut(s) 181, 232, 237, 342, 1128
EclXI CGGCCG 1 cut(s) 787
Eco130I CCWWGG 2 cut(s) 870, 931
Eco24I GRGCYC 2 cut(s) 282, 297
Eco47I GGWCC 1 cut(s) 862
Eco52I CGGCCG 1 cut(s) 787
Eco72I CACGTG 1 cut(s) 1020
Eco81I CCTNAGG 1 cut(s) 1100
Eco88I CYCGRG 1 cut(s) 281
EcoT14I CCWWGG 2 cut(s) 870, 931
EcoT22I ATGCAT 1 cut(s) 692
EcoT38I GRGCYC 2 cut(s) 282, 297
EgeI GGCGCC 1 cut(s) 375
EheI GGCGCC 1 cut(s) 375
ErhI CCWWGG 2 cut(s) 870, 931
Esp3I CGTCTC 1 cut(s) 1130
FauI CCCGC 1 cut(s) 1053
FblI GTMKAC 1 cut(s) 1026
Fnu4HI GCNGC 3 cut(s) 565, 787, 809
FokI GGATG 3 cut(s) 131, 182, 677
FriOI GRGCYC 2 cut(s) 282, 297
Fsp4HI GCNGC 3 cut(s) 565, 787, 809
FspBI CTAG 2 cut(s) 452, 632
GlaI GCGC 3 cut(s) 375, 836, 998
GluI GCNGC 3 cut(s) 565, 787, 809
GsuI CTGGAG 1 cut(s) 379
HaeII RGCGCY 1 cut(s) 377
HaeIII GGCC 4 cut(s) 564, 789, 907, 1044
HapII CCGG 4 cut(s) 317, 1009, 1057, 1189
HgaI GACGC 1 cut(s) 792
HhaI GCGC 3 cut(s) 376, 837, 999
Hin1I GRCGYC 1 cut(s) 374
Hin6I GCGC 3 cut(s) 374, 835, 997
HinP1I GCGC 3 cut(s) 374, 835, 997
HincII GTYRAC 1 cut(s) 1027
HindII GTYRAC 1 cut(s) 1027
HindIII AAGCTT 3 cut(s) 65, 301, 323
HinfI GANTC 7 cut(s) 471, 548, 666, 683, 755, 897, 1012
HpaII CCGG 4 cut(s) 317, 1009, 1057, 1189
HphI GGTGA 4 cut(s) 39, 700, 1087, 1124
Hpy166II GTNNAC 5 cut(s) 511, 604, 718, 862, 1027
Hpy188I TCNGA 5 cut(s) 277, 538, 682, 892, 1226
Hpy188III TCNNGA 6 cut(s) 358, 385, 430, 542, 878, 1184
Hpy8I GTNNAC 5 cut(s) 511, 604, 718, 862, 1027
Hpy99I CGWCG 1 cut(s) 950
HpyAV CCTTC 6 cut(s) 158, 249, 273, 282, 482, 531
HpyCH4III ACNGT 3 cut(s) 715, 866, 1213
HpyCH4IV ACGT 4 cut(s) 507, 750, 1019, 1207
HpyCH4V TGCA 3 cut(s) 331, 690, 808
HpyF10VI GCNNNNNNNGC 6 cut(s) 201, 292, 371, 783, 795, 834
HpyF3I CTNAG 6 cut(s) 191, 210, 276, 378, 1100, 1196
HpySE526I ACGT 4 cut(s) 507, 750, 1019, 1207
Hsp92I GRCGYC 1 cut(s) 374
HspAI GCGC 3 cut(s) 374, 835, 997
KasI GGCGCC 1 cut(s) 373
Kzo9I GATC 4 cut(s) 16, 381, 538, 852
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 1 cut(s) 848
Lsp1109I GCAGC 1 cut(s) 820
LweI GCATC 3 cut(s) 153, 204, 699
MaeI CTAG 2 cut(s) 452, 632
MaeII ACGT 4 cut(s) 507, 750, 1019, 1207
MaeIII GTNAC 1 cut(s) 1075
MalI GATC 4 cut(s) 18, 383, 540, 854
MboI GATC 4 cut(s) 16, 381, 538, 852
MhlI GDGCHC 3 cut(s) 282, 297, 954
MluCI AATT 2 cut(s) 181, 388
Mly113I GGCGCC 1 cut(s) 374
MlyI GAGTC 2 cut(s) 764, 1006
MmeI TCCRAC 3 cut(s) 223, 760, 1029
Mph1103I ATGCAT 1 cut(s) 692
MroXI GAANNNNTTC 1 cut(s) 351
MseI TTAA 1 cut(s) 732
MslI CAYNNNNRTG 1 cut(s) 428
MspI CCGG 4 cut(s) 317, 1009, 1057, 1189
MvnI CGCG 1 cut(s) 786
MwoI GCNNNNNNNGC 6 cut(s) 201, 292, 371, 783, 795, 834
NarI GGCGCC 1 cut(s) 374
NcoI CCATGG 2 cut(s) 870, 931
NdeII GATC 4 cut(s) 16, 381, 538, 852
NlaIV GGNNCC 2 cut(s) 375, 844
NmuCI GTSAC 1 cut(s) 1075
NsiI ATGCAT 1 cut(s) 692
NspI RCATGY 1 cut(s) 818
PagI TCATGA 1 cut(s) 429
PciI ACATGT 1 cut(s) 814
PciSI GCTCTTC 1 cut(s) 342
PcsI WCGNNNNNNNCGW 1 cut(s) 666
PdmI GAANNNNTTC 1 cut(s) 351
PfeI GAWTC 5 cut(s) 471, 548, 666, 683, 897
PinAI ACCGGT 2 cut(s) 1056, 1188
PkrI GCNGC 3 cut(s) 566, 788, 810
PleI GAGTC 2 cut(s) 763, 1006
PluTI GGCGCC 1 cut(s) 377
PmaCI CACGTG 1 cut(s) 1020
PmlI CACGTG 1 cut(s) 1020
PpsI GAGTC 2 cut(s) 763, 1006
Ppu21I YACGTR 2 cut(s) 1020, 1208
PscI ACATGT 1 cut(s) 814
PsiI TTATAA 1 cut(s) 528
PspCI CACGTG 1 cut(s) 1020
PspN4I GGNNCC 2 cut(s) 375, 844
PspPI GGNCC 2 cut(s) 862, 1043
PsrI GAACNNNNNNTAC 4 cut(s) 612, 644, 654, 686
RsaI GTAC 2 cut(s) 672, 1210
RsaNI GTAC 2 cut(s) 671, 1209
RseI CAYNNNNRTG 1 cut(s) 428
SalI GTCGAC 1 cut(s) 1025
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 1 cut(s) 732
SatI GCNGC 3 cut(s) 565, 787, 809
Sau3AI GATC 4 cut(s) 16, 381, 538, 852
Sau96I GGNCC 2 cut(s) 862, 1043
SchI GAGTC 2 cut(s) 764, 1006
SduI GDGCHC 3 cut(s) 282, 297, 954
SfaNI GCATC 3 cut(s) 153, 204, 699
SfoI GGCGCC 1 cut(s) 375
SinI GGWCC 1 cut(s) 862
SmiMI CAYNNNNRTG 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 878
SmoI CTYRAG 1 cut(s) 878
Sse9I AATT 2 cut(s) 181, 388
SsiI CCGC 5 cut(s) 129, 565, 786, 1046, 1082
SspDI GGCGCC 1 cut(s) 373
SspI AATATT 1 cut(s) 1144
SspMI CTAG 2 cut(s) 452, 632
StyI CCWWGG 2 cut(s) 870, 931
TaaI ACNGT 3 cut(s) 715, 866, 1213
TaiI ACGT 4 cut(s) 510, 753, 1022, 1210
TaqI TCGA 5 cut(s) 403, 855, 1026, 1034, 1185
TasI AATT 2 cut(s) 181, 388
TauI GCSGC 2 cut(s) 567, 789
TfiI GAWTC 5 cut(s) 471, 548, 666, 683, 897
Tru1I TTAA 1 cut(s) 732
Tru9I TTAA 1 cut(s) 732
TscAI CASTG 2 cut(s) 718, 836
TseFI GTSAC 1 cut(s) 1075
TseI GCWGC 1 cut(s) 808
Tsp45I GTSAC 1 cut(s) 1075
TspDTI ATGAA 6 cut(s) 17, 33, 412, 418, 501, 1078
TspGWI ACGGA 2 cut(s) 541, 548
TspRI CASTG 2 cut(s) 718, 836
VpaK11BI GGWCC 1 cut(s) 862
XapI RAATTY 1 cut(s) 181
XceI RCATGY 1 cut(s) 818
XmiI GTMKAC 1 cut(s) 1026
XmnI GAANNNNTTC 1 cut(s) 351
XspI CTAG 2 cut(s) 452, 632
Zsp2I ATGCAT 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.