RchiOBHm_Chr4g0434731

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001447
Physical Location & Seq
Reverse (-)
58416930 .. 58418252
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40318

Sequence Viewer

Length: 1212 bp
ATGAAGGATCGAAATCTATGCCCACTAAACTTGTTGTCAAAAGTCTTCAATGTCAAACACCACCTCCTCAATCTCGTTTCTCATTTTCTATTCTTCATTGGTGGTTTCACCCTTGGAGTATTACTCACCTCCTCATATCTAAGAAACACTTCATTAACTTTACAAATAGACCAATTCTCCATAGCTACTTCATCACTTCCATCCAATAATTCTAATTCACAAATGTTAGTGCCAACAAATGTAAAGGCCCCAACATCCTATATGAGCCATGTTCGTATAGGACTAAAAGCATATCTGGAGCCGCCTAATGTTACGCACGACATGAATGATGAAGAATTGCTATGGAGAGCTTCGACGGCTCCTCGGATTTCTGAATATCCATTCCATCGAGTCCCAAAAGTTGCTTTCATGTTCTTGACAAAGGGACCTGTACATTTGGCCCCATTGTGGGAGAAGTTCTTCAAAGGGCATCAAGGCTTGTACTCAATTTATGTTCACTCAGATCCATCTTACAATGCATCATCACACCCAGAAAGTCCTGTTTTTCAAGGCAGAAGAATTCCAAGTCAGAAAGTAAAATGGGCACAGGTGAACATGATTGAGGCTGAAAGCCGCCTAATTGCGAATGCTCTTCTCGATATCTCAAACCAGCGCTTCGTTCTCCTCTCGGAAGCATGCATTCCCCTATACAACTTCTCCACTATCTACACTTACCTCCTAAACTCCCAAGAAACTTTTGTGGAGGTCTATGATGATCCAAGTTCAGTTGGACGCGGCCGATATAATTCAAGTTACTACCCAATATTGGATCAGTGGAGGAAAGGGTCACAGTGGGTGGAAATTGATAGAGACATTGCCATTGAAGTTGTCTCGGATCGGACATACTTGCCAGCCTTCCGGTTGGGCAACCGCTCTTGTTTTGCTGATGAACATTACTCGCCAACATTTGTGAACCTAAAATTTTCGGCAAAGAATTTAAACAGGAGTGTCACCTGGGTTAATTGGTCTACTCGTGGCCCGCACCCTGTCGAGTACAATAGCTCATCGGCCGAGCTTCTGAACACCCTGCGCAATGGTAATGGTCAGAGATGTTTATATAATCGAAGGAGAAGCAATGTTTATTTCTTATTCGCCAGGAAGTCCCGGCCTTCTGCTTTGGATAGCCTTCTCAGGCTTGCCCCCAAGATCATGCACTTCAACACAGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

403

Amino Acids

46.21

Weight (kDa)

9.47

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 134 - 354 2.4e-70 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1070
AccBSI CCGCTC 1 cut(s) 912
AccI GTMKAC 1 cut(s) 1007
AccII CGCG 1 cut(s) 774
AciI CCGC 5 cut(s) 302, 613, 774, 910, 1019
AclWI GGATC 5 cut(s) 15, 497, 749, 816, 882
AcoI YGGCCR 2 cut(s) 775, 1047
AcsI RAATTY 3 cut(s) 558, 959, 973
AfaI GTAC 3 cut(s) 432, 482, 1034
AfeI AGCGCT 1 cut(s) 653
AfiI CCNNNNNNNGG 3 cut(s) 447, 448, 805
AgsI TTSAA 6 cut(s) 49, 463, 548, 789, 863, 1198
AjnI CCWGG 2 cut(s) 992, 1133
AluBI AGCT 4 cut(s) 185, 350, 1041, 1054
AluI AGCT 4 cut(s) 185, 350, 1041, 1054
Alw26I GTCTC 2 cut(s) 843, 874
AlwI GGATC 5 cut(s) 15, 497, 749, 816, 882
Aor51HI AGCGCT 1 cut(s) 653
AoxI GGCC 6 cut(s) 246, 438, 775, 1015, 1047, 1145
ApoI RAATTY 3 cut(s) 558, 959, 973
Asp700I GAANNNNTTC 2 cut(s) 148, 458
AspLEI GCGC 2 cut(s) 654, 1071
AspS9I GGNCC 4 cut(s) 247, 425, 439, 1016
AsuC2I CCSGG 1 cut(s) 1144
AsuHPI GGTGA 4 cut(s) 100, 118, 601, 982
AvaII GGWCC 1 cut(s) 425
BaeGI GKGCMC 1 cut(s) 586
BauI CACGAG 1 cut(s) 1011
BbsI GAAGAC 1 cut(s) 37
BccI CCATC 3 cut(s) 208, 393, 514
BceAI ACGGC 1 cut(s) 372
BcgI CGANNNNNNTGC 1 cut(s) 34
BciT130I CCWGG 2 cut(s) 994, 1135
BcnI CCSGG 1 cut(s) 1144
BcoDI GTCTC 2 cut(s) 843, 874
BfoI RGCGCY 1 cut(s) 655
BisI GCNGC 3 cut(s) 302, 613, 775
BlsI GCNGC 3 cut(s) 303, 614, 776
Bme1390I CCNGG 3 cut(s) 994, 1135, 1144
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 4 cut(s) 247, 425, 439, 1016
BmiI GGNNCC 5 cut(s) 249, 300, 360, 426, 441
BmrFI CCNGG 3 cut(s) 994, 1135, 1144
BmsI GCATC 2 cut(s) 478, 527
BpiI GAAGAC 1 cut(s) 37
BplI GAGNNNNNCTC 2 cut(s) 108, 140
BpmI CTGGAG 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 1144
BsaBI GATNNNNATC 1 cut(s) 12
BsaJI CCNNGG 3 cut(s) 112, 362, 993
BsaWI WCCGGW 1 cut(s) 897
BsaXI ACNNNNNCTCC 6 cut(s) 48, 78, 161, 191, 680, 710
Bsc4I CCNNNNNNNGG 3 cut(s) 447, 448, 805
Bse3DI GCAATG 3 cut(s) 852, 1078, 1120
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 2 cut(s) 994, 1135
BseDI CCNNGG 3 cut(s) 112, 362, 993
BseGI GGATG 2 cut(s) 200, 254
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 3 cut(s) 447, 448, 805
BseMI GCAATG 3 cut(s) 852, 1078, 1120
BseMII CTCAG 2 cut(s) 513, 1183
BseRI GAGGAG 4 cut(s) 56, 121, 351, 653
BseSI GKGCMC 1 cut(s) 586
BseX3I CGGCCG 2 cut(s) 775, 1047
Bsh1236I CGCG 1 cut(s) 774
Bsh1285I CGRYCG 2 cut(s) 778, 1050
BshFI GGCC 6 cut(s) 248, 440, 777, 1017, 1049, 1147
BsiEI CGRYCG 2 cut(s) 778, 1050
BsiSI CCGG 2 cut(s) 898, 1144
BslFI GGGAC 3 cut(s) 377, 438, 1126
BslI CCNNNNNNNGG 3 cut(s) 447, 448, 805
BsmAI GTCTC 2 cut(s) 843, 874
BsmFI GGGAC 3 cut(s) 377, 438, 1126
BsmI GAATGC 2 cut(s) 631, 678
BsnI GGCC 6 cut(s) 248, 440, 777, 1017, 1049, 1147
Bsp1286I GDGCHC 1 cut(s) 586
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 6 cut(s) 7, 502, 754, 808, 874, 1185
BspACI CCGC 5 cut(s) 302, 613, 774, 910, 1019
BspANI GGCC 6 cut(s) 248, 440, 777, 1017, 1049, 1147
BspCNI CTCAG 2 cut(s) 512, 1182
BspFNI CGCG 1 cut(s) 774
BspLI GGNNCC 5 cut(s) 249, 300, 360, 426, 441
BspPI GGATC 5 cut(s) 15, 497, 749, 816, 882
BspQI GCTCTTC 1 cut(s) 636
BsrBI CCGCTC 1 cut(s) 912
BsrDI GCAATG 3 cut(s) 852, 1078, 1120
BsrGI TGTACA 1 cut(s) 430
BssECI CCNNGG 3 cut(s) 112, 362, 993
BssMI GATC 6 cut(s) 7, 502, 754, 808, 874, 1185
BssSI CACGAG 1 cut(s) 1011
BssT1I CCWWGG 1 cut(s) 112
Bst2BI CACGAG 1 cut(s) 1011
Bst2UI CCWGG 2 cut(s) 994, 1135
Bst4CI ACNGT 1 cut(s) 831
Bst6I CTCTTC 1 cut(s) 636
BstAUI TGTACA 1 cut(s) 430
BstC8I GCNNGC 4 cut(s) 676, 891, 1019, 1176
BstDEI CTNAG 3 cut(s) 140, 499, 1169
BstF5I GGATG 2 cut(s) 200, 254
BstFNI CGCG 1 cut(s) 774
BstH2I RGCGCY 1 cut(s) 655
BstHHI GCGC 2 cut(s) 654, 1071
BstKTI GATC 6 cut(s) 10, 505, 757, 811, 877, 1188
BstMAI GTCTC 2 cut(s) 843, 874
BstMBI GATC 6 cut(s) 7, 502, 754, 808, 874, 1185
BstMCI CGRYCG 2 cut(s) 778, 1050
BstMWI GCNNNNNNNGC 1 cut(s) 356
BstNI CCWGG 2 cut(s) 994, 1135
BstNSI RCATGY 1 cut(s) 678
BstSCI CCNGG 3 cut(s) 992, 1133, 1142
BstSLI GKGCMC 1 cut(s) 586
BstUI CGCG 1 cut(s) 774
BstV2I GAAGAC 1 cut(s) 37
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
BstZI CGGCCG 2 cut(s) 775, 1047
BsuRI GGCC 6 cut(s) 248, 440, 777, 1017, 1049, 1147
BtsCI GGATG 2 cut(s) 200, 254
BtsIMutI CAGTG 2 cut(s) 818, 836
Cac8I GCNNGC 4 cut(s) 676, 891, 1019, 1176
CfoI GCGC 2 cut(s) 654, 1071
Cfr13I GGNCC 4 cut(s) 247, 425, 439, 1016
CseI GACGC 1 cut(s) 780
Csp6I GTAC 3 cut(s) 431, 481, 1033
CspCI CAANNNNNGTGG 2 cut(s) 12, 47
CviAII CATG 6 cut(s) 269, 322, 409, 595, 675, 1189
CviQI GTAC 3 cut(s) 431, 481, 1033
DdeI CTNAG 3 cut(s) 140, 499, 1169
DpnI GATC 6 cut(s) 9, 504, 756, 810, 876, 1187
DpnII GATC 6 cut(s) 7, 502, 754, 808, 874, 1185
DraI TTTAAA 1 cut(s) 978
EaeI YGGCCR 2 cut(s) 775, 1047
EagI CGGCCG 2 cut(s) 775, 1047
Eam1104I CTCTTC 1 cut(s) 636
EarI CTCTTC 1 cut(s) 636
EclXI CGGCCG 2 cut(s) 775, 1047
Eco130I CCWWGG 1 cut(s) 112
Eco32I GATATC 1 cut(s) 640
Eco47I GGWCC 1 cut(s) 425
Eco47III AGCGCT 1 cut(s) 653
Eco52I CGGCCG 2 cut(s) 775, 1047
EcoO109I RGGNCCY 2 cut(s) 247, 425
EcoRI GAATTC 1 cut(s) 558
EcoRII CCWGG 2 cut(s) 992, 1133
EcoRV GATATC 1 cut(s) 640
EcoT14I CCWWGG 1 cut(s) 112
EcoT22I ATGCAT 2 cut(s) 520, 680
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 6 cut(s) 272, 325, 412, 598, 678, 1192
FalI AAGNNNNNCTT 2 cut(s) 133, 165
FaqI GGGAC 3 cut(s) 377, 438, 1126
FatI CATG 6 cut(s) 268, 321, 408, 594, 674, 1188
FauI CCCGC 1 cut(s) 1026
FblI GTMKAC 1 cut(s) 1007
Fnu4HI GCNGC 3 cut(s) 302, 613, 775
FokI GGATG 2 cut(s) 187, 241
Fsp4HI GCNGC 3 cut(s) 302, 613, 775
FspI TGCGCA 1 cut(s) 1070
GlaI GCGC 2 cut(s) 653, 1070
GluI GCNGC 3 cut(s) 302, 613, 775
GsuI CTGGAG 1 cut(s) 317
HaeII RGCGCY 1 cut(s) 655
HaeIII GGCC 6 cut(s) 248, 440, 777, 1017, 1049, 1147
HapII CCGG 2 cut(s) 898, 1144
HgaI GACGC 1 cut(s) 780
HhaI GCGC 2 cut(s) 654, 1071
Hin1II CATG 6 cut(s) 272, 325, 412, 598, 678, 1192
Hin6I GCGC 2 cut(s) 652, 1069
HinP1I GCGC 2 cut(s) 652, 1069
HinfI GANTC 2 cut(s) 390, 1205
HpaII CCGG 2 cut(s) 898, 1144
HphI GGTGA 4 cut(s) 100, 118, 601, 982
Hpy166II GTNNAC 4 cut(s) 496, 592, 952, 1008
Hpy188I TCNGA 9 cut(s) 366, 373, 502, 570, 670, 874, 879, 1059, 1086
Hpy188III TCNNGA 3 cut(s) 296, 415, 635
Hpy8I GTNNAC 4 cut(s) 496, 592, 952, 1008
Hpy99I CGWCG 1 cut(s) 358
HpyAV CCTTC 4 cut(s) 904, 1098, 1158, 1175
HpyCH4III ACNGT 1 cut(s) 831
HpyCH4V TGCA 3 cut(s) 518, 678, 1192
HpyF10VI GCNNNNNNNGC 1 cut(s) 356
HpyF3I CTNAG 3 cut(s) 140, 499, 1169
Hsp92II CATG 6 cut(s) 272, 325, 412, 598, 678, 1192
HspAI GCGC 2 cut(s) 652, 1069
Kzo9I GATC 6 cut(s) 7, 502, 754, 808, 874, 1185
LguI GCTCTTC 1 cut(s) 636
LmnI GCTCC 2 cut(s) 298, 364
LweI GCATC 2 cut(s) 478, 527
MaeIII GTNAC 4 cut(s) 310, 791, 825, 988
MalI GATC 6 cut(s) 9, 504, 756, 810, 876, 1187
MbiI CCGCTC 1 cut(s) 912
MboI GATC 6 cut(s) 7, 502, 754, 808, 874, 1185
MboII GAAGA 6 cut(s) 37, 85, 344, 451, 567, 623
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 1 cut(s) 586
MlyI GAGTC 1 cut(s) 399
MmeI TCCRAC 1 cut(s) 748
Mph1103I ATGCAT 2 cut(s) 520, 680
MroXI GAANNNNTTC 2 cut(s) 148, 458
MseI TTAA 4 cut(s) 155, 977, 999, 1210
MspI CCGG 2 cut(s) 898, 1144
MspR9I CCNGG 3 cut(s) 994, 1135, 1144
Mva1269I GAATGC 2 cut(s) 631, 678
MvaI CCWGG 2 cut(s) 994, 1135
MvnI CGCG 1 cut(s) 774
MwoI GCNNNNNNNGC 1 cut(s) 356
NciI CCSGG 1 cut(s) 1144
NdeII GATC 6 cut(s) 7, 502, 754, 808, 874, 1185
NlaIII CATG 6 cut(s) 272, 325, 412, 598, 678, 1192
NlaIV GGNNCC 5 cut(s) 249, 300, 360, 426, 441
NmeAIII GCCGAG 1 cut(s) 1075
NmuCI GTSAC 2 cut(s) 825, 988
NsbI TGCGCA 1 cut(s) 1070
NsiI ATGCAT 2 cut(s) 520, 680
NspI RCATGY 1 cut(s) 678
PaeI GCATGC 1 cut(s) 678
PciSI GCTCTTC 1 cut(s) 636
PctI GAATGC 2 cut(s) 631, 678
PdmI GAANNNNTTC 2 cut(s) 148, 458
PfeI GAWTC 1 cut(s) 1205
PkrI GCNGC 3 cut(s) 303, 614, 776
PleI GAGTC 1 cut(s) 398
PpsI GAGTC 1 cut(s) 398
PpuMI RGGWCCY 1 cut(s) 425
Psp5II RGGWCCY 1 cut(s) 425
Psp6I CCWGG 2 cut(s) 992, 1133
PspGI CCWGG 2 cut(s) 992, 1133
PspN4I GGNNCC 5 cut(s) 249, 300, 360, 426, 441
PspPI GGNCC 4 cut(s) 247, 425, 439, 1016
PspPPI RGGWCCY 1 cut(s) 425
PsuI RGATCY 1 cut(s) 502
RsaI GTAC 3 cut(s) 432, 482, 1034
RsaNI GTAC 3 cut(s) 431, 481, 1033
SapI GCTCTTC 1 cut(s) 636
SaqAI TTAA 4 cut(s) 155, 977, 999, 1210
SatI GCNGC 3 cut(s) 302, 613, 775
Sau3AI GATC 6 cut(s) 7, 502, 754, 808, 874, 1185
Sau96I GGNCC 4 cut(s) 247, 425, 439, 1016
SchI GAGTC 1 cut(s) 399
ScrFI CCNGG 3 cut(s) 994, 1135, 1144
SduI GDGCHC 1 cut(s) 586
SfaNI GCATC 2 cut(s) 478, 527
SinI GGWCC 1 cut(s) 425
SphI GCATGC 1 cut(s) 678
SsiI CCGC 5 cut(s) 302, 613, 774, 910, 1019
SspI AATATT 1 cut(s) 804
StyD4I CCNGG 3 cut(s) 992, 1133, 1142
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 1 cut(s) 831
TaqI TCGA 6 cut(s) 10, 353, 388, 636, 1029, 1102
TatI WGTACW 3 cut(s) 430, 480, 1032
TauI GCSGC 3 cut(s) 304, 615, 777
TfiI GAWTC 1 cut(s) 1205
Tru1I TTAA 4 cut(s) 155, 977, 999, 1210
Tru9I TTAA 4 cut(s) 155, 977, 999, 1210
TscAI CASTG 2 cut(s) 818, 836
TseFI GTSAC 2 cut(s) 825, 988
Tsp45I GTSAC 2 cut(s) 825, 988
TspDTI ATGAA 8 cut(s) 17, 85, 141, 180, 338, 345, 397, 942
TspRI CASTG 2 cut(s) 818, 836
VpaK11BI GGWCC 1 cut(s) 425
XapI RAATTY 3 cut(s) 558, 959, 973
XceI RCATGY 1 cut(s) 678
XmiI GTMKAC 1 cut(s) 1007
XmnI GAANNNNTTC 2 cut(s) 148, 458
Zsp2I ATGCAT 2 cut(s) 520, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.