MD13G1037500.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001447
Physical Location & Seq
Reverse (-)
2574847 .. 2576416
1570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1037500.v1.1.491

Sequence Viewer

Length: 849 bp
ATGCCAAATGCAACCTCCCCAACAACATCTGTTTCGTCAAGTCCTCCGCGGACAGGGTTGAAGGATTATCTGAAGCCACCGAACGCATTTCACGACATGAACAATGCAGAACTGCTGTGGAGAGCTTCTATAACTCCGCAGATTCCTGAATATCCATTCCATTTTGTTCCAAAAGTTGCTTTCATGTTCTTGACAAAGGGCCCTGTTGTTTTGGCACCACTGTGGGAGAAGTTCTTTGCCGGGCATCAAGGTTTGTACTCAATTTATGTGCACTCAAGTCCGTCCTACAATCACTCATTGTACACCGAAACCCCAGTTTTTGGTGGCCGGCGAATTCCAAGTCAGGGAGTAGAATGGGGGAAGGTGAGCCTGATCGCGGCAGAGCGCCTCCTCCTGGCGAATGCTCTTCTCGACGTCTCAAACCAGCGTTTTGTTCTGCTCTCAGAATCATGCATTCCCCTCCATAACTTCTCCACTGTCTACTCCTACCTCATGAACTCCACCCAAACTTTCGTTGAGGTCTTTGACGATCCCAGTGGAGTAGGACGCGGCCGGTATGAACTGAGTGACTACCGGGGAATCACAATAGATCAGTGGCGAAAAGGGTCGCGGTGGTTCCAGGCTGATCGAGACCTCGCCCTTGAATTCGTATCGGATCGAATATACTTCCCAGTGTTCATGAAATGCAAAGGCTACTGTTTCGCCGATGAACATTATTTGCCAACGTTTGTGTACATAAAATTTGGAGCAAAGAATGCAAATAGGACGTTGACCTGGTTGACTGAAAGGGGAGACTTTAAGGAATCAACCTCCTGGGCACCGAAGTGCTCTTTCGATCCCATGACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

283

Amino Acids

32.18

Weight (kDa)

8.26

Isoelectric Point (pI)

32.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 59 - 260 2.6e-63 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 417
AccB1I GGYRCC 2 cut(s) 214, 817
AccB7I CCANNNNNTGG 1 cut(s) 320
AccI GTMKAC 1 cut(s) 480
AccII CGCG 4 cut(s) 49, 377, 549, 610
AciI CCGC 6 cut(s) 47, 49, 137, 377, 549, 610
AclI AACGTT 1 cut(s) 725
AclWI GGATC 3 cut(s) 524, 663, 830
AcoI YGGCCR 2 cut(s) 325, 550
AcsI RAATTY 3 cut(s) 333, 644, 740
AcuI CTGAAG 1 cut(s) 92
AcyI GRCGYC 1 cut(s) 414
AfaI GTAC 3 cut(s) 257, 302, 734
AfiI CCNNNNNNNGG 5 cut(s) 53, 320, 344, 376, 394
AgsI TTSAA 2 cut(s) 61, 644
AjnI CCWGG 4 cut(s) 393, 618, 773, 812
AleI CACNNNNGTG 2 cut(s) 220, 823
AluBI AGCT 1 cut(s) 125
AluI AGCT 1 cut(s) 125
Alw21I GWGCWC 2 cut(s) 273, 830
Alw26I GTCTC 3 cut(s) 421, 624, 786
Alw44I GTGCAC 1 cut(s) 269
AlwI GGATC 3 cut(s) 524, 663, 830
AoxI GGCC 3 cut(s) 199, 325, 550
ApaI GGGCCC 1 cut(s) 203
ApaLI GTGCAC 1 cut(s) 269
ApoI RAATTY 3 cut(s) 333, 644, 740
ArsI GACNNNNNNTTYG 2 cut(s) 325, 357
AspLEI GCGC 1 cut(s) 387
AspS9I GGNCC 2 cut(s) 199, 200
AsuC2I CCSGG 2 cut(s) 241, 575
AsuHPI GGTGA 1 cut(s) 376
BaeGI GKGCMC 3 cut(s) 203, 273, 820
BanI GGYRCC 2 cut(s) 214, 817
BanII GRGCYC 1 cut(s) 203
Bbv12I GWGCWC 2 cut(s) 273, 830
BciT130I CCWGG 4 cut(s) 395, 620, 775, 814
BcnI CCSGG 2 cut(s) 241, 575
BcoDI GTCTC 3 cut(s) 421, 624, 786
BfaI CTAG 1 cut(s) 847
BfoI RGCGCY 1 cut(s) 388
BisI GCNGC 2 cut(s) 378, 550
BlsI GCNGC 2 cut(s) 379, 551
Bme1390I CCNGG 6 cut(s) 241, 395, 575, 620, 775, 814
BmgT120I GGNCC 2 cut(s) 199, 200
BmiI GGNNCC 4 cut(s) 201, 216, 617, 819
BmrFI CCNGG 6 cut(s) 241, 395, 575, 620, 775, 814
BmrI ACTGGG 3 cut(s) 308, 528, 665
BmsI GCATC 1 cut(s) 253
BmuI ACTGGG 3 cut(s) 308, 528, 665
BpuEI CTTGAG 1 cut(s) 259
BpuMI CCSGG 2 cut(s) 241, 575
BsaHI GRCGYC 1 cut(s) 414
BsaI GGTCTC 1 cut(s) 624
BsaJI CCNNGG 3 cut(s) 47, 574, 813
Bsc4I CCNNNNNNNGG 5 cut(s) 53, 320, 344, 376, 394
Bse118I RCCGGY 2 cut(s) 327, 552
Bse1I ACTGG 3 cut(s) 314, 534, 671
BseBI CCWGG 4 cut(s) 395, 620, 775, 814
BseDI CCNNGG 3 cut(s) 47, 574, 813
BseLI CCNNNNNNNGG 5 cut(s) 53, 320, 344, 376, 394
BseMII CTCAG 2 cut(s) 456, 554
BseNI ACTGG 3 cut(s) 314, 534, 671
BseRI GAGGAG 1 cut(s) 380
BseSI GKGCMC 3 cut(s) 203, 273, 820
BseX3I CGGCCG 1 cut(s) 550
Bsh1236I CGCG 4 cut(s) 49, 377, 549, 610
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 3 cut(s) 201, 327, 552
BshNI GGYRCC 2 cut(s) 214, 817
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 2 cut(s) 273, 830
BsiSI CCGG 4 cut(s) 240, 328, 553, 574
BslI CCNNNNNNNGG 5 cut(s) 53, 320, 344, 376, 394
BsmAI GTCTC 3 cut(s) 421, 624, 786
BsmBI CGTCTC 1 cut(s) 421
BsmI GAATGC 3 cut(s) 406, 453, 760
BsnI GGCC 3 cut(s) 201, 327, 552
Bso31I GGTCTC 1 cut(s) 624
Bsp120I GGGCCC 1 cut(s) 199
Bsp1286I GDGCHC 4 cut(s) 203, 273, 820, 830
Bsp1407I TGTACA 2 cut(s) 300, 732
Bsp143I GATC 6 cut(s) 372, 529, 589, 625, 655, 835
BspACI CCGC 6 cut(s) 47, 49, 137, 377, 549, 610
BspANI GGCC 3 cut(s) 201, 327, 552
BspCNI CTCAG 2 cut(s) 455, 555
BspFNI CGCG 4 cut(s) 49, 377, 549, 610
BspHI TCATGA 2 cut(s) 492, 678
BspLI GGNNCC 4 cut(s) 201, 216, 617, 819
BspPI GGATC 3 cut(s) 524, 663, 830
BspQI GCTCTTC 1 cut(s) 411
BspT107I GGYRCC 2 cut(s) 214, 817
BspTNI GGTCTC 1 cut(s) 624
BsrFI RCCGGY 2 cut(s) 327, 552
BsrGI TGTACA 2 cut(s) 300, 732
BsrI ACTGG 3 cut(s) 314, 534, 671
BssAI RCCGGY 2 cut(s) 327, 552
BssECI CCNNGG 3 cut(s) 47, 574, 813
BssMI GATC 6 cut(s) 372, 529, 589, 625, 655, 835
BssNI GRCGYC 1 cut(s) 414
Bst2UI CCWGG 4 cut(s) 395, 620, 775, 814
Bst4CI ACNGT 3 cut(s) 222, 478, 698
Bst6I CTCTTC 1 cut(s) 411
BstACI GRCGYC 1 cut(s) 414
BstAPI GCANNNNNTGC 1 cut(s) 755
BstAUI TGTACA 2 cut(s) 300, 732
BstC8I GCNNGC 1 cut(s) 329
BstDEI CTNAG 2 cut(s) 442, 563
BstDSI CCRYGG 1 cut(s) 47
BstFNI CGCG 4 cut(s) 49, 377, 549, 610
BstH2I RGCGCY 1 cut(s) 388
BstHHI GCGC 1 cut(s) 387
BstKTI GATC 6 cut(s) 375, 532, 592, 628, 658, 838
BstMAI GTCTC 3 cut(s) 421, 624, 786
BstMBI GATC 6 cut(s) 372, 529, 589, 625, 655, 835
BstMCI CGRYCG 1 cut(s) 553
BstMWI GCNNNNNNNGC 1 cut(s) 755
BstNI CCWGG 4 cut(s) 395, 620, 775, 814
BstSCI CCNGG 6 cut(s) 239, 393, 573, 618, 773, 812
BstSLI GKGCMC 3 cut(s) 203, 273, 820
BstUI CGCG 4 cut(s) 49, 377, 549, 610
BstZI CGGCCG 1 cut(s) 550
BsuRI GGCC 3 cut(s) 201, 327, 552
BtgI CCRYGG 1 cut(s) 47
BtsIMutI CAGTG 5 cut(s) 218, 474, 541, 599, 678
Cac8I GCNNGC 1 cut(s) 329
CciI TCATGA 2 cut(s) 492, 678
CfoI GCGC 1 cut(s) 387
Cfr10I RCCGGY 2 cut(s) 327, 552
Cfr13I GGNCC 2 cut(s) 199, 200
Cfr42I CCGCGG 1 cut(s) 50
CseI GACGC 1 cut(s) 555
CsiI ACCWGGT 1 cut(s) 773
Csp6I GTAC 3 cut(s) 256, 301, 733
CviAII CATG 6 cut(s) 97, 184, 450, 493, 679, 841
CviJI RGCY 8 cut(s) 76, 125, 201, 327, 369, 552, 623, 693
CviKI_1 RGCY 8 cut(s) 76, 125, 201, 327, 369, 552, 623, 693
CviQI GTAC 3 cut(s) 256, 301, 733
DdeI CTNAG 2 cut(s) 442, 563
DpnI GATC 6 cut(s) 374, 531, 591, 627, 657, 837
DpnII GATC 6 cut(s) 372, 529, 589, 625, 655, 835
EaeI YGGCCR 2 cut(s) 325, 550
EagI CGGCCG 1 cut(s) 550
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
EclXI CGGCCG 1 cut(s) 550
Eco24I GRGCYC 1 cut(s) 203
Eco31I GGTCTC 1 cut(s) 624
Eco52I CGGCCG 1 cut(s) 550
Eco57I CTGAAG 1 cut(s) 92
EcoO109I RGGNCCY 2 cut(s) 199, 200
EcoRI GAATTC 2 cut(s) 333, 644
EcoRII CCWGG 4 cut(s) 393, 618, 773, 812
EcoT22I ATGCAT 1 cut(s) 455
EcoT38I GRGCYC 1 cut(s) 203
Esp3I CGTCTC 1 cut(s) 421
FaeI CATG 6 cut(s) 100, 187, 453, 496, 682, 844
FatI CATG 6 cut(s) 96, 183, 449, 492, 678, 840
FblI GTMKAC 1 cut(s) 480
Fnu4HI GCNGC 2 cut(s) 378, 550
FriOI GRGCYC 1 cut(s) 203
Fsp4HI GCNGC 2 cut(s) 378, 550
FspBI CTAG 1 cut(s) 847
GlaI GCGC 1 cut(s) 386
GluI GCNGC 2 cut(s) 378, 550
HaeII RGCGCY 1 cut(s) 388
HaeIII GGCC 3 cut(s) 201, 327, 552
HapII CCGG 4 cut(s) 240, 328, 553, 574
HgaI GACGC 1 cut(s) 555
HhaI GCGC 1 cut(s) 387
Hin1I GRCGYC 1 cut(s) 414
Hin1II CATG 6 cut(s) 100, 187, 453, 496, 682, 844
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HincII GTYRAC 2 cut(s) 771, 780
HindII GTYRAC 2 cut(s) 771, 780
HinfI GANTC 4 cut(s) 142, 446, 579, 803
HpaII CCGG 4 cut(s) 240, 328, 553, 574
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 6 cut(s) 271, 303, 481, 733, 771, 780
Hpy188I TCNGA 3 cut(s) 72, 445, 655
Hpy188III TCNNGA 7 cut(s) 92, 146, 190, 410, 493, 629, 679
Hpy8I GTNNAC 6 cut(s) 271, 303, 481, 733, 771, 780
Hpy99I CGWCG 1 cut(s) 416
HpyAV CCTTC 2 cut(s) 55, 355
HpyCH4III ACNGT 3 cut(s) 222, 478, 698
HpyCH4IV ACGT 3 cut(s) 414, 725, 767
HpyCH4V TGCA 6 cut(s) 11, 107, 271, 453, 687, 758
HpyF10VI GCNNNNNNNGC 1 cut(s) 755
HpyF3I CTNAG 2 cut(s) 442, 563
HpySE526I ACGT 3 cut(s) 414, 725, 767
Hsp92I GRCGYC 1 cut(s) 414
Hsp92II CATG 6 cut(s) 100, 187, 453, 496, 682, 844
HspAI GCGC 1 cut(s) 385
KroI GCCGGC 1 cut(s) 327
KroNI GCCGGC 1 cut(s) 329
KspI CCGCGG 1 cut(s) 50
Kzo9I GATC 6 cut(s) 372, 529, 589, 625, 655, 835
LguI GCTCTTC 1 cut(s) 411
LmnI GCTCC 1 cut(s) 746
LweI GCATC 1 cut(s) 253
MabI ACCWGGT 1 cut(s) 773
MaeI CTAG 1 cut(s) 847
MaeII ACGT 3 cut(s) 414, 725, 767
MaeIII GTNAC 1 cut(s) 566
MalI GATC 6 cut(s) 374, 531, 591, 627, 657, 837
MboI GATC 6 cut(s) 372, 529, 589, 625, 655, 835
MboII GAAGA 1 cut(s) 398
MhlI GDGCHC 4 cut(s) 203, 273, 820, 830
MluCI AATT 4 cut(s) 261, 333, 644, 740
MnlI CCTC 9 cut(s) 25, 54, 398, 401, 470, 500, 511, 644, 820
Mph1103I ATGCAT 1 cut(s) 455
MroNI GCCGGC 1 cut(s) 327
MseI TTAA 1 cut(s) 798
MslI CAYNNNNRTG 2 cut(s) 220, 823
MspA1I CMGCKG 1 cut(s) 49
MspI CCGG 4 cut(s) 240, 328, 553, 574
MspR9I CCNGG 6 cut(s) 241, 395, 575, 620, 775, 814
Mva1269I GAATGC 3 cut(s) 406, 453, 760
MvaI CCWGG 4 cut(s) 395, 620, 775, 814
MvnI CGCG 4 cut(s) 49, 377, 549, 610
MwoI GCNNNNNNNGC 1 cut(s) 755
NaeI GCCGGC 1 cut(s) 329
NciI CCSGG 2 cut(s) 241, 575
NdeII GATC 6 cut(s) 372, 529, 589, 625, 655, 835
NgoMIV GCCGGC 1 cut(s) 327
NlaIII CATG 6 cut(s) 100, 187, 453, 496, 682, 844
NlaIV GGNNCC 4 cut(s) 201, 216, 617, 819
NmuCI GTSAC 1 cut(s) 566
NsiI ATGCAT 1 cut(s) 455
OliI CACNNNNGTG 2 cut(s) 220, 823
PagI TCATGA 2 cut(s) 492, 678
PciSI GCTCTTC 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 3 cut(s) 406, 453, 760
PdiI GCCGGC 1 cut(s) 329
PfeI GAWTC 4 cut(s) 142, 446, 579, 803
PflMI CCANNNNNTGG 1 cut(s) 320
PkrI GCNGC 2 cut(s) 379, 551
Psp1406I AACGTT 1 cut(s) 725
Psp6I CCWGG 4 cut(s) 393, 618, 773, 812
PspGI CCWGG 4 cut(s) 393, 618, 773, 812
PspN4I GGNNCC 4 cut(s) 201, 216, 617, 819
PspOMI GGGCCC 1 cut(s) 199
PspPI GGNCC 2 cut(s) 199, 200
RsaI GTAC 3 cut(s) 257, 302, 734
RsaNI GTAC 3 cut(s) 256, 301, 733
RseI CAYNNNNRTG 2 cut(s) 220, 823
SacII CCGCGG 1 cut(s) 50
SapI GCTCTTC 1 cut(s) 411
SaqAI TTAA 1 cut(s) 798
SatI GCNGC 2 cut(s) 378, 550
Sau3AI GATC 6 cut(s) 372, 529, 589, 625, 655, 835
Sau96I GGNCC 2 cut(s) 199, 200
ScrFI CCNGG 6 cut(s) 241, 395, 575, 620, 775, 814
SduI GDGCHC 4 cut(s) 203, 273, 820, 830
SexAI ACCWGGT 1 cut(s) 773
SfaNI GCATC 1 cut(s) 253
Sfr303I CCGCGG 1 cut(s) 50
SgrBI CCGCGG 1 cut(s) 50
SmiMI CAYNNNNRTG 2 cut(s) 220, 823
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
Sse9I AATT 4 cut(s) 261, 333, 644, 740
SsiI CCGC 6 cut(s) 47, 49, 137, 377, 549, 610
SspMI CTAG 1 cut(s) 847
StyD4I CCNGG 6 cut(s) 239, 393, 573, 618, 773, 812
TaaI ACNGT 3 cut(s) 222, 478, 698
TaiI ACGT 3 cut(s) 417, 728, 770
TaqI TCGA 4 cut(s) 411, 628, 658, 834
TasI AATT 4 cut(s) 261, 333, 644, 740
TatI WGTACW 3 cut(s) 255, 300, 732
TauI GCSGC 2 cut(s) 380, 552
TfiI GAWTC 4 cut(s) 142, 446, 579, 803
Tru1I TTAA 1 cut(s) 798
Tru9I TTAA 1 cut(s) 798
TscAI CASTG 5 cut(s) 225, 481, 541, 599, 678
TseFI GTSAC 1 cut(s) 566
Tsp45I GTSAC 1 cut(s) 566
TspDTI ATGAA 7 cut(s) 113, 172, 509, 573, 667, 695, 723
TspGWI ACGGA 1 cut(s) 270
TspRI CASTG 5 cut(s) 225, 481, 541, 599, 678
Van91I CCANNNNNTGG 1 cut(s) 320
VneI GTGCAC 1 cut(s) 269
XapI RAATTY 3 cut(s) 333, 644, 740
XmiI GTMKAC 1 cut(s) 480
XspI CTAG 1 cut(s) 847
ZraI GACGTC 1 cut(s) 415
Zsp2I ATGCAT 1 cut(s) 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.