Rroxscaffold_5G00375940

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001447
Physical Location & Seq
Reverse (-)
56705968 .. 56707263
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375940.1

Sequence Viewer

Length: 1218 bp
ATGAAGGATCGAAATCTATGCCCGCTAAACTTGTTGTCAAAAGTCTTCAATGTCAAACACCACCTCCTCATTCTCGTTTCTCATTTTCTGTTCTTCATTGGTGGTTTCACCCTTGGAGTATTAATCACCTCCTCATATCTAAGAAACACTTCATTAACTTTACAAATAGACCAATTCTCCATAGCTACTTCATCACTTCCATCCAATAATTCTAATTCACAAATGTTAGTGCCAACAAATGTAAAGGCCCCAACATCCTACATGAGCCATGTTCGTATAGGACTAAAAGCATATCTGGAGCCGCCTAATGTTATGCACGACATGAATGATGAAGAATTGCTATGGAGAGCTTCGACGGCTCCTCGGATTTCTGAATATCCATTCCATCGAGTCCCAAAAGTTGCTTTCATGTTCTTGACAAAGGGACCTGTACATTTGGCCCCATTGTGGGAGAAGTTCTTCAAAGGGCATCAAGGCTTGTACTCAATTTATGTTCACTCAGATCCATCTTACAATGCATCATCACACCCAGAAAGTCCTGTTTTTCAAGGCCGAAGAATTCCAAGTCAGAAAGTAAAATGGGCACAGGTGAACATGATTGAGGCTGAAAGCCGCCTAATTGCGAATGCTCTTCTTGATATCTCAAACCAGCGCTTCGTTCTCCTCTCGGAAGCATGCATTCCCCTATACAACTTCTCCACTATCTACTCTTACCTCCTAAACTCCCAAGAAACTTTTGTGGAGGTCTATGATGATCCAAGTTCAGTTGGACGCGGCCGATATAATTCAAGTTACTACCCAATAATCACATTGGATCAGTGGAGGAAAGGGTCACAGTGGGTTGAAATTGATAGAGACATTGCCATTGAAGTTGTCTCGGATCGGACATACTTACCAGCCTTCCGGTTGGGCAACCGCTCTTGTTTTGCTGATGAGCATTACTTGCCAACATTTGTGAACCTAAAATTTTCGGCAAAGAATTCAAACAGGAGTGTCACCTGGGTTGATTGGTCTACGCGTGGCCCGCACCCTGTCGAGTACAATAGCACATCGGCTGAGCTTCTGAACACCCTGCGCAATGGTAATGGTCAGAGATGTTTATATAATCGAAGGAGAAGCAATGTTTGTTTCTTATTCGCCAGGAAGTTCCGGCCTTCTGCTTTGGATAACCTTCTCAGGCTTGCCCCCAAGATCATGCACTTCAACACAGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

405

Amino Acids

46.48

Weight (kDa)

9.37

Isoelectric Point (pI)

46.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 134 - 357 9.1e-74 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1076
AccBSI CCGCTC 1 cut(s) 918
AccI GTMKAC 1 cut(s) 1013
AccII CGCG 2 cut(s) 774, 1018
AciI CCGC 6 cut(s) 23, 302, 613, 774, 916, 1025
AclWI GGATC 5 cut(s) 15, 497, 749, 822, 888
AcoI YGGCCR 1 cut(s) 775
AcsI RAATTY 3 cut(s) 558, 965, 979
AfaI GTAC 3 cut(s) 432, 482, 1040
AfeI AGCGCT 1 cut(s) 653
AfiI CCNNNNNNNGG 2 cut(s) 447, 448
AflIII ACRYGT 1 cut(s) 1016
AgsI TTSAA 8 cut(s) 49, 463, 548, 789, 845, 869, 984, 1204
AjnI CCWGG 2 cut(s) 998, 1139
AluBI AGCT 3 cut(s) 185, 350, 1060
AluI AGCT 3 cut(s) 185, 350, 1060
Alw26I GTCTC 2 cut(s) 849, 880
AlwI GGATC 5 cut(s) 15, 497, 749, 822, 888
Aor51HI AGCGCT 1 cut(s) 653
AoxI GGCC 6 cut(s) 246, 438, 550, 775, 1021, 1151
ApoI RAATTY 3 cut(s) 558, 965, 979
AseI ATTAAT 1 cut(s) 122
Asp700I GAANNNNTTC 2 cut(s) 148, 458
AspLEI GCGC 2 cut(s) 654, 1077
AspS9I GGNCC 4 cut(s) 247, 425, 439, 1022
AsuHPI GGTGA 4 cut(s) 100, 118, 601, 988
AvaII GGWCC 1 cut(s) 425
BaeGI GKGCMC 1 cut(s) 586
BbsI GAAGAC 1 cut(s) 37
BccI CCATC 3 cut(s) 208, 393, 514
BceAI ACGGC 1 cut(s) 372
BcgI CGANNNNNNTGC 1 cut(s) 34
BciT130I CCWGG 2 cut(s) 1000, 1141
BcoDI GTCTC 2 cut(s) 849, 880
BfoI RGCGCY 1 cut(s) 655
BisI GCNGC 3 cut(s) 302, 613, 775
BlpI GCTNAGC 1 cut(s) 1056
BlsI GCNGC 3 cut(s) 303, 614, 776
Bme1390I CCNGG 2 cut(s) 1000, 1141
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 4 cut(s) 247, 425, 439, 1022
BmiI GGNNCC 5 cut(s) 249, 300, 360, 426, 441
BmrFI CCNGG 2 cut(s) 1000, 1141
BmsI GCATC 2 cut(s) 478, 527
BpiI GAAGAC 1 cut(s) 37
BpmI CTGGAG 1 cut(s) 317
Bpu1102I GCTNAGC 1 cut(s) 1056
BsaBI GATNNNNATC 1 cut(s) 12
BsaJI CCNNGG 3 cut(s) 112, 362, 999
BsaWI WCCGGW 1 cut(s) 903
BsaXI ACNNNNNCTCC 6 cut(s) 48, 78, 161, 191, 680, 710
Bsc4I CCNNNNNNNGG 2 cut(s) 447, 448
Bse3DI GCAATG 3 cut(s) 858, 1084, 1126
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 2 cut(s) 1000, 1141
BseDI CCNNGG 3 cut(s) 112, 362, 999
BseGI GGATG 2 cut(s) 200, 254
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 2 cut(s) 447, 448
BseMI GCAATG 3 cut(s) 858, 1084, 1126
BseMII CTCAG 3 cut(s) 513, 1047, 1189
BseRI GAGGAG 4 cut(s) 56, 121, 351, 653
BseSI GKGCMC 1 cut(s) 586
BseX3I CGGCCG 1 cut(s) 775
Bsh1236I CGCG 2 cut(s) 774, 1018
Bsh1285I CGRYCG 1 cut(s) 778
BshFI GGCC 6 cut(s) 248, 440, 552, 777, 1023, 1153
BsiEI CGRYCG 1 cut(s) 778
BsiSI CCGG 2 cut(s) 904, 1150
BslFI GGGAC 2 cut(s) 377, 438
BslI CCNNNNNNNGG 2 cut(s) 447, 448
BsmAI GTCTC 2 cut(s) 849, 880
BsmFI GGGAC 2 cut(s) 377, 438
BsmI GAATGC 2 cut(s) 631, 678
BsnI GGCC 6 cut(s) 248, 440, 552, 777, 1023, 1153
Bsp1286I GDGCHC 1 cut(s) 586
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 6 cut(s) 7, 502, 754, 814, 880, 1191
Bsp1720I GCTNAGC 1 cut(s) 1056
BspACI CCGC 6 cut(s) 23, 302, 613, 774, 916, 1025
BspANI GGCC 6 cut(s) 248, 440, 552, 777, 1023, 1153
BspCNI CTCAG 3 cut(s) 512, 1048, 1188
BspFNI CGCG 2 cut(s) 774, 1018
BspLI GGNNCC 5 cut(s) 249, 300, 360, 426, 441
BspPI GGATC 5 cut(s) 15, 497, 749, 822, 888
BspQI GCTCTTC 1 cut(s) 636
BsrBI CCGCTC 1 cut(s) 918
BsrDI GCAATG 3 cut(s) 858, 1084, 1126
BsrGI TGTACA 1 cut(s) 430
BssECI CCNNGG 3 cut(s) 112, 362, 999
BssMI GATC 6 cut(s) 7, 502, 754, 814, 880, 1191
BssT1I CCWWGG 1 cut(s) 112
Bst2UI CCWGG 2 cut(s) 1000, 1141
Bst4CI ACNGT 1 cut(s) 837
Bst6I CTCTTC 1 cut(s) 636
BstAPI GCANNNNNTGC 1 cut(s) 943
BstAUI TGTACA 1 cut(s) 430
BstC8I GCNNGC 4 cut(s) 23, 676, 1025, 1182
BstDEI CTNAG 4 cut(s) 140, 499, 1056, 1175
BstF5I GGATG 2 cut(s) 200, 254
BstFNI CGCG 2 cut(s) 774, 1018
BstH2I RGCGCY 1 cut(s) 655
BstHHI GCGC 2 cut(s) 654, 1077
BstKTI GATC 6 cut(s) 10, 505, 757, 817, 883, 1194
BstMAI GTCTC 2 cut(s) 849, 880
BstMBI GATC 6 cut(s) 7, 502, 754, 814, 880, 1191
BstMCI CGRYCG 1 cut(s) 778
BstMWI GCNNNNNNNGC 3 cut(s) 356, 943, 1024
BstNI CCWGG 2 cut(s) 1000, 1141
BstNSI RCATGY 1 cut(s) 678
BstSCI CCNGG 2 cut(s) 998, 1139
BstSLI GKGCMC 1 cut(s) 586
BstUI CGCG 2 cut(s) 774, 1018
BstV2I GAAGAC 1 cut(s) 37
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
BstZI CGGCCG 1 cut(s) 775
BsuRI GGCC 6 cut(s) 248, 440, 552, 777, 1023, 1153
BtsCI GGATG 2 cut(s) 200, 254
BtsIMutI CAGTG 2 cut(s) 824, 842
Cac8I GCNNGC 4 cut(s) 23, 676, 1025, 1182
CfoI GCGC 2 cut(s) 654, 1077
Cfr13I GGNCC 4 cut(s) 247, 425, 439, 1022
CseI GACGC 1 cut(s) 780
Csp6I GTAC 3 cut(s) 431, 481, 1039
CviAII CATG 7 cut(s) 262, 269, 322, 409, 595, 675, 1195
CviQI GTAC 3 cut(s) 431, 481, 1039
DdeI CTNAG 4 cut(s) 140, 499, 1056, 1175
DpnI GATC 6 cut(s) 9, 504, 756, 816, 882, 1193
DpnII GATC 6 cut(s) 7, 502, 754, 814, 880, 1191
EaeI YGGCCR 1 cut(s) 775
EagI CGGCCG 1 cut(s) 775
Eam1104I CTCTTC 1 cut(s) 636
EarI CTCTTC 1 cut(s) 636
EclXI CGGCCG 1 cut(s) 775
Eco130I CCWWGG 1 cut(s) 112
Eco32I GATATC 1 cut(s) 640
Eco47I GGWCC 1 cut(s) 425
Eco47III AGCGCT 1 cut(s) 653
Eco52I CGGCCG 1 cut(s) 775
EcoO109I RGGNCCY 2 cut(s) 247, 425
EcoRI GAATTC 2 cut(s) 558, 979
EcoRII CCWGG 2 cut(s) 998, 1139
EcoRV GATATC 1 cut(s) 640
EcoT14I CCWWGG 1 cut(s) 112
EcoT22I ATGCAT 2 cut(s) 520, 680
ErhI CCWWGG 1 cut(s) 112
FaeI CATG 7 cut(s) 265, 272, 325, 412, 598, 678, 1198
FalI AAGNNNNNCTT 2 cut(s) 133, 165
FaqI GGGAC 2 cut(s) 377, 438
FatI CATG 7 cut(s) 261, 268, 321, 408, 594, 674, 1194
FauI CCCGC 2 cut(s) 30, 1032
FblI GTMKAC 1 cut(s) 1013
Fnu4HI GCNGC 3 cut(s) 302, 613, 775
FokI GGATG 2 cut(s) 187, 241
Fsp4HI GCNGC 3 cut(s) 302, 613, 775
FspI TGCGCA 1 cut(s) 1076
GlaI GCGC 2 cut(s) 653, 1076
GluI GCNGC 3 cut(s) 302, 613, 775
GsuI CTGGAG 1 cut(s) 317
HaeII RGCGCY 1 cut(s) 655
HaeIII GGCC 6 cut(s) 248, 440, 552, 777, 1023, 1153
HapII CCGG 2 cut(s) 904, 1150
HgaI GACGC 1 cut(s) 780
HhaI GCGC 2 cut(s) 654, 1077
Hin1II CATG 7 cut(s) 265, 272, 325, 412, 598, 678, 1198
Hin6I GCGC 2 cut(s) 652, 1075
HinP1I GCGC 2 cut(s) 652, 1075
HinfI GANTC 2 cut(s) 390, 1211
HpaII CCGG 2 cut(s) 904, 1150
HphI GGTGA 4 cut(s) 100, 118, 601, 988
Hpy166II GTNNAC 4 cut(s) 496, 592, 958, 1014
Hpy188I TCNGA 9 cut(s) 366, 373, 502, 570, 670, 880, 885, 1065, 1092
Hpy188III TCNNGA 3 cut(s) 296, 415, 635
Hpy8I GTNNAC 4 cut(s) 496, 592, 958, 1014
Hpy99I CGWCG 1 cut(s) 358
HpyAV CCTTC 4 cut(s) 910, 1104, 1164, 1181
HpyCH4III ACNGT 1 cut(s) 837
HpyCH4V TGCA 4 cut(s) 316, 518, 678, 1198
HpyF10VI GCNNNNNNNGC 3 cut(s) 356, 943, 1024
HpyF3I CTNAG 4 cut(s) 140, 499, 1056, 1175
Hsp92II CATG 7 cut(s) 265, 272, 325, 412, 598, 678, 1198
HspAI GCGC 2 cut(s) 652, 1075
Kzo9I GATC 6 cut(s) 7, 502, 754, 814, 880, 1191
LguI GCTCTTC 1 cut(s) 636
LmnI GCTCC 2 cut(s) 298, 364
LweI GCATC 2 cut(s) 478, 527
MaeIII GTNAC 3 cut(s) 791, 831, 994
MalI GATC 6 cut(s) 9, 504, 756, 816, 882, 1193
MbiI CCGCTC 1 cut(s) 918
MboI GATC 6 cut(s) 7, 502, 754, 814, 880, 1191
MboII GAAGA 6 cut(s) 37, 85, 344, 451, 567, 623
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 1 cut(s) 586
MluI ACGCGT 1 cut(s) 1016
MlyI GAGTC 1 cut(s) 399
MmeI TCCRAC 1 cut(s) 748
Mph1103I ATGCAT 2 cut(s) 520, 680
MroXI GAANNNNTTC 2 cut(s) 148, 458
MseI TTAA 3 cut(s) 122, 155, 1216
MspI CCGG 2 cut(s) 904, 1150
MspR9I CCNGG 2 cut(s) 1000, 1141
Mva1269I GAATGC 2 cut(s) 631, 678
MvaI CCWGG 2 cut(s) 1000, 1141
MvnI CGCG 2 cut(s) 774, 1018
MwoI GCNNNNNNNGC 3 cut(s) 356, 943, 1024
NdeII GATC 6 cut(s) 7, 502, 754, 814, 880, 1191
NlaIII CATG 7 cut(s) 265, 272, 325, 412, 598, 678, 1198
NlaIV GGNNCC 5 cut(s) 249, 300, 360, 426, 441
NmuCI GTSAC 2 cut(s) 831, 994
NsbI TGCGCA 1 cut(s) 1076
NsiI ATGCAT 2 cut(s) 520, 680
NspI RCATGY 1 cut(s) 678
PaeI GCATGC 1 cut(s) 678
PciSI GCTCTTC 1 cut(s) 636
PctI GAATGC 2 cut(s) 631, 678
PdmI GAANNNNTTC 2 cut(s) 148, 458
PfeI GAWTC 1 cut(s) 1211
PkrI GCNGC 3 cut(s) 303, 614, 776
PleI GAGTC 1 cut(s) 398
PpsI GAGTC 1 cut(s) 398
PpuMI RGGWCCY 1 cut(s) 425
PshBI ATTAAT 1 cut(s) 122
Psp5II RGGWCCY 1 cut(s) 425
Psp6I CCWGG 2 cut(s) 998, 1139
PspGI CCWGG 2 cut(s) 998, 1139
PspN4I GGNNCC 5 cut(s) 249, 300, 360, 426, 441
PspPI GGNCC 4 cut(s) 247, 425, 439, 1022
PspPPI RGGWCCY 1 cut(s) 425
PsuI RGATCY 1 cut(s) 502
RsaI GTAC 3 cut(s) 432, 482, 1040
RsaNI GTAC 3 cut(s) 431, 481, 1039
SapI GCTCTTC 1 cut(s) 636
SaqAI TTAA 3 cut(s) 122, 155, 1216
SatI GCNGC 3 cut(s) 302, 613, 775
Sau3AI GATC 6 cut(s) 7, 502, 754, 814, 880, 1191
Sau96I GGNCC 4 cut(s) 247, 425, 439, 1022
SchI GAGTC 1 cut(s) 399
ScrFI CCNGG 2 cut(s) 1000, 1141
SduI GDGCHC 1 cut(s) 586
SfaNI GCATC 2 cut(s) 478, 527
SinI GGWCC 1 cut(s) 425
SphI GCATGC 1 cut(s) 678
SsiI CCGC 6 cut(s) 23, 302, 613, 774, 916, 1025
StyD4I CCNGG 2 cut(s) 998, 1139
StyI CCWWGG 1 cut(s) 112
TaaI ACNGT 1 cut(s) 837
TaqI TCGA 5 cut(s) 10, 353, 388, 1035, 1108
TatI WGTACW 3 cut(s) 430, 480, 1038
TauI GCSGC 3 cut(s) 304, 615, 777
TfiI GAWTC 1 cut(s) 1211
Tru1I TTAA 3 cut(s) 122, 155, 1216
Tru9I TTAA 3 cut(s) 122, 155, 1216
TscAI CASTG 2 cut(s) 824, 842
TseFI GTSAC 2 cut(s) 831, 994
Tsp45I GTSAC 2 cut(s) 831, 994
TspDTI ATGAA 7 cut(s) 17, 85, 141, 180, 338, 345, 397
TspRI CASTG 2 cut(s) 824, 842
VpaK11BI GGWCC 1 cut(s) 425
VspI ATTAAT 1 cut(s) 122
XapI RAATTY 3 cut(s) 558, 965, 979
XceI RCATGY 1 cut(s) 678
XmiI GTMKAC 1 cut(s) 1013
XmnI GAANNNNTTC 2 cut(s) 148, 458
Zsp2I ATGCAT 2 cut(s) 520, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.