Rroxscaffold_5G00375950

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001447
Physical Location & Seq
Reverse (-)
56742644 .. 56744412
1769 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375950.1

Sequence Viewer

Length: 1257 bp
ATGAAGGATCATAATCAAAACCCTTCAAACTTCTTCACAAAACTAATCAATGTCCGAGGCCACCTCCTCAATCTCCTCTCTTATTTCCTCATCTTTGGTGGTGGTTTAGTTCTCGGAATCGTACTTGCCTCTTCTTATCTCAAAGACTCTTCCTTTAGTCTACAAATAGCCCAACTATCATTCTCCTCTTCTTCTTCTTCTTCTTCATTATCTCCGTCCAATAGTTCCAATATTTCACATATGACAATTAGTCCAAATGACACAGTCACGATGTCTAACATGACCGAAGTTCATATAGGAGAATTAGAGGCATTTCTAAAGCCGCCTAAGGTTCGGCACGACATGGACGATGTGGAATTGTTCTGGAGAGCTTCCATGGCCCCTCGGATACCCGAATATCCGTTCCACCGAGTTCCGAAAGTTGCCTTCATGTTCTTGACAAGGGGACCTGTTCATTTGGCTCCATTGTGGGAGAAGTTCTTCCAAGGGCACCAAGGCTTGTTCTCAATCTATGTCCACTCAGATCCGTCTTATAATGAATCGTCTCACCCTGAAAGCCCAGTCTTTCATGGCCGAAGAATTCAAGTCAGGAGAGCAGAATGGGGGCAAGTGAACATGATTGAAGCCGAGAGCCGCCTAATTGCGAATGCTCTTCTCGACATCTCAAACCAACGTTTTGTTCTGCTTTCAGAATCATGCATCCCCCTCTTCAATTTCTCCACAGTCTACTCTTACCTCATAAACACCAACAAGACTTTTGTACAGGTCTATGATGATCCAAGCGGTGTTGGGCGTGGCCGTTATGATGAAAGCTACTACCCTCAAATCACATTGGACCAGTGGAGGAAAGGGTCGCAGTGGGTTGAAATAGATAGAGACCTTGCCATTGAAGTTGTTTCTGATCAAAAATACTACACTACTTTCCAGAAACTCTGCAAAGGCTATTGCTTTGCAGATGAGCATTACTTGCCAACATTTATGAACATAAGATTTCCGAAAAAGAATTCAAACAGGACTGTGACCTGGGTTGATTGGTCTAGGGGCGGACCTCATCCTGTCGAGTACGCGAGCACAGATGTCACGGTTGAGCTTCTGAATACTCTGAGGAAGGGTAACGGCTGGTGTGATTACAATGGAAGGGGCACAGATGTTTGTTTCTTGTTCGCCCGGAAGTTCTCGCTAAATTCTTTGGATAAGCTGCTGAGCATTGCACCACTGTTAATGCAATTCAACACAGACATTGATAAAGAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

418

Amino Acids

47.88

Weight (kDa)

6.67

Isoelectric Point (pI)

38.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 141 - 368 1.7e-76 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 534
AccB1I GGYRCC 1 cut(s) 489
AccI GTMKAC 2 cut(s) 160, 726
AccII CGCG 1 cut(s) 1067
AciI CCGC 4 cut(s) 323, 634, 783, 1044
AclI AACGTT 1 cut(s) 673
AclWI GGATC 3 cut(s) 15, 518, 770
AcoI YGGCCR 2 cut(s) 571, 796
AcsI RAATTY 3 cut(s) 579, 1003, 1183
AfaI GTAC 4 cut(s) 123, 762, 1064, 1253
AgsI TTSAA 8 cut(s) 27, 584, 623, 712, 866, 890, 1008, 1231
AhdI GACNNNNNGTC 1 cut(s) 249
AjnI CCWGG 1 cut(s) 1022
AjuI GAANNNNNNNTTGG 2 cut(s) 663, 695
AluBI AGCT 4 cut(s) 371, 813, 1090, 1198
AluI AGCT 4 cut(s) 371, 813, 1090, 1198
Alw21I GWGCWC 1 cut(s) 1073
Alw26I GTCTC 2 cut(s) 549, 870
AlwI GGATC 3 cut(s) 15, 518, 770
AoxI GGCC 4 cut(s) 58, 378, 571, 796
ApeKI GCWGC 1 cut(s) 1198
ApoI RAATTY 3 cut(s) 579, 1003, 1183
Asp700I GAANNNNTTC 1 cut(s) 479
AspS9I GGNCC 4 cut(s) 379, 446, 835, 1046
AsuC2I CCSGG 1 cut(s) 1168
AsuHPI GGTGA 1 cut(s) 539
AvaII GGWCC 3 cut(s) 446, 835, 1046
AxyI CCTNAGG 1 cut(s) 327
BaeGI GKGCMC 2 cut(s) 492, 1145
BanI GGYRCC 1 cut(s) 489
Bbv12I GWGCWC 1 cut(s) 1073
BbvI GCAGC 1 cut(s) 1185
BceAI ACGGC 2 cut(s) 783, 1132
BciT130I CCWGG 1 cut(s) 1024
BciVI GTATCC 1 cut(s) 381
BclI TGATCA 1 cut(s) 901
BcnI CCSGG 1 cut(s) 1168
BcoDI GTCTC 2 cut(s) 549, 870
BfaI CTAG 2 cut(s) 1038, 1255
BfuI GTATCC 1 cut(s) 381
BisI GCNGC 3 cut(s) 323, 634, 1199
BlpI GCTNAGC 1 cut(s) 1202
BlsI GCNGC 3 cut(s) 324, 635, 1200
BmcAI AGTACT 1 cut(s) 1253
Bme1390I CCNGG 2 cut(s) 1024, 1168
Bme18I GGWCC 3 cut(s) 446, 835, 1046
BmeRI GACNNNNNGTC 1 cut(s) 249
BmgT120I GGNCC 4 cut(s) 379, 446, 835, 1046
BmiI GGNNCC 4 cut(s) 381, 447, 462, 491
BmrFI CCNGG 2 cut(s) 1024, 1168
BmrI ACTGGG 1 cut(s) 554
BmsI GCATC 1 cut(s) 708
BmuI ACTGGG 1 cut(s) 554
BplI GAGNNNNNCTC 2 cut(s) 48, 80
BpmI CTGGAG 1 cut(s) 385
Bpu1102I GCTNAGC 1 cut(s) 1202
BpuMI CCSGG 1 cut(s) 1168
BsaBI GATNNNNATC 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 870
BsaJI CCNNGG 6 cut(s) 55, 375, 383, 484, 493, 1023
Bse1I ACTGG 2 cut(s) 560, 838
Bse21I CCTNAGG 1 cut(s) 327
Bse3DI GCAATG 1 cut(s) 1206
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 1 cut(s) 1024
BseDI CCNNGG 6 cut(s) 55, 375, 383, 484, 493, 1023
BseGI GGATG 2 cut(s) 699, 1051
BseJI GATNNNNATC 1 cut(s) 12
BseMI GCAATG 1 cut(s) 1206
BseMII CTCAG 3 cut(s) 534, 1094, 1193
BseNI ACTGG 2 cut(s) 560, 838
BseRI GAGGAG 3 cut(s) 56, 65, 175
BseSI GKGCMC 2 cut(s) 492, 1145
BseXI GCAGC 1 cut(s) 1185
Bsh1236I CGCG 1 cut(s) 1067
BshFI GGCC 4 cut(s) 60, 380, 573, 798
BshNI GGYRCC 1 cut(s) 489
BsiHKAI GWGCWC 1 cut(s) 1073
BsiSI CCGG 1 cut(s) 1168
BslFI GGGAC 1 cut(s) 459
BsmAI GTCTC 2 cut(s) 549, 870
BsmBI CGTCTC 1 cut(s) 549
BsmFI GGGAC 1 cut(s) 459
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 4 cut(s) 60, 380, 573, 798
Bso31I GGTCTC 1 cut(s) 870
Bsp1286I GDGCHC 3 cut(s) 492, 1073, 1145
Bsp1407I TGTACA 1 cut(s) 760
Bsp143I GATC 4 cut(s) 7, 523, 775, 901
Bsp1720I GCTNAGC 1 cut(s) 1202
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 4 cut(s) 323, 634, 783, 1044
BspANI GGCC 4 cut(s) 60, 380, 573, 798
BspCNI CTCAG 3 cut(s) 533, 1095, 1194
BspFNI CGCG 1 cut(s) 1067
BspLI GGNNCC 4 cut(s) 381, 447, 462, 491
BspPI GGATC 3 cut(s) 15, 518, 770
BspQI GCTCTTC 1 cut(s) 657
BspT107I GGYRCC 1 cut(s) 489
BspTNI GGTCTC 1 cut(s) 870
BsrDI GCAATG 1 cut(s) 1206
BsrGI TGTACA 1 cut(s) 760
BsrI ACTGG 2 cut(s) 560, 838
BssECI CCNNGG 6 cut(s) 55, 375, 383, 484, 493, 1023
BssMI GATC 4 cut(s) 7, 523, 775, 901
BssT1I CCWWGG 3 cut(s) 375, 484, 493
Bst2UI CCWGG 1 cut(s) 1024
Bst4CI ACNGT 5 cut(s) 265, 724, 1018, 1084, 1218
Bst6I CTCTTC 5 cut(s) 136, 154, 193, 657, 713
BstAPI GCANNNNNTGC 1 cut(s) 967
BstAUI TGTACA 1 cut(s) 760
BstC8I GCNNGC 1 cut(s) 1069
BstDEI CTNAG 4 cut(s) 327, 520, 1103, 1202
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 699, 1051
BstFNI CGCG 1 cut(s) 1067
BstKTI GATC 4 cut(s) 10, 526, 778, 904
BstMAI GTCTC 2 cut(s) 549, 870
BstMBI GATC 4 cut(s) 7, 523, 775, 901
BstMWI GCNNNNNNNGC 2 cut(s) 377, 967
BstNI CCWGG 1 cut(s) 1024
BstSCI CCNGG 2 cut(s) 1022, 1166
BstSLI GKGCMC 2 cut(s) 492, 1145
BstUI CGCG 1 cut(s) 1067
BstV1I GCAGC 1 cut(s) 1185
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
Bsu36I CCTNAGG 1 cut(s) 327
BsuI GTATCC 1 cut(s) 381
BsuRI GGCC 4 cut(s) 60, 380, 573, 798
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 2 cut(s) 699, 1051
BtsI GCAGTG 1 cut(s) 863
BtsIMutI CAGTG 3 cut(s) 845, 863, 1214
Cac8I GCNNGC 1 cut(s) 1069
Cfr13I GGNCC 4 cut(s) 379, 446, 835, 1046
Csp6I GTAC 4 cut(s) 122, 761, 1063, 1252
CviAII CATG 7 cut(s) 280, 343, 376, 430, 569, 616, 696
CviQI GTAC 4 cut(s) 122, 761, 1063, 1252
DdeI CTNAG 4 cut(s) 327, 520, 1103, 1202
DpnI GATC 4 cut(s) 9, 525, 777, 903
DpnII GATC 4 cut(s) 7, 523, 775, 901
DriI GACNNNNNGTC 1 cut(s) 249
EaeI YGGCCR 2 cut(s) 571, 796
Eam1104I CTCTTC 5 cut(s) 136, 154, 193, 657, 713
Eam1105I GACNNNNNGTC 1 cut(s) 249
EarI CTCTTC 5 cut(s) 136, 154, 193, 657, 713
EciI GGCGGA 1 cut(s) 1059
Eco130I CCWWGG 3 cut(s) 375, 484, 493
Eco31I GGTCTC 1 cut(s) 870
Eco47I GGWCC 3 cut(s) 446, 835, 1046
Eco81I CCTNAGG 1 cut(s) 327
EcoO109I RGGNCCY 1 cut(s) 446
EcoRI GAATTC 2 cut(s) 579, 1003
EcoRII CCWGG 1 cut(s) 1022
EcoT14I CCWWGG 3 cut(s) 375, 484, 493
EcoT22I ATGCAT 1 cut(s) 701
ErhI CCWWGG 3 cut(s) 375, 484, 493
Esp3I CGTCTC 1 cut(s) 549
FaeI CATG 7 cut(s) 283, 346, 379, 433, 572, 619, 699
FaqI GGGAC 1 cut(s) 459
FatI CATG 7 cut(s) 279, 342, 375, 429, 568, 615, 695
FauNDI CATATG 1 cut(s) 240
FbaI TGATCA 1 cut(s) 901
FblI GTMKAC 2 cut(s) 160, 726
Fnu4HI GCNGC 3 cut(s) 323, 634, 1199
FokI GGATG 2 cut(s) 686, 1038
Fsp4HI GCNGC 3 cut(s) 323, 634, 1199
FspBI CTAG 2 cut(s) 1038, 1255
GluI GCNGC 3 cut(s) 323, 634, 1199
GsuI CTGGAG 1 cut(s) 385
HaeIII GGCC 4 cut(s) 60, 380, 573, 798
HapII CCGG 1 cut(s) 1168
Hin1II CATG 7 cut(s) 283, 346, 379, 433, 572, 619, 699
HinfI GANTC 4 cut(s) 117, 146, 539, 692
HpaII CCGG 1 cut(s) 1168
HphI GGTGA 1 cut(s) 539
Hpy166II GTNNAC 4 cut(s) 161, 517, 613, 727
Hpy188III TCNNGA 6 cut(s) 268, 364, 436, 589, 656, 925
Hpy8I GTNNAC 4 cut(s) 161, 517, 613, 727
HpyAV CCTTC 4 cut(s) 33, 436, 1102, 1131
HpyCH4III ACNGT 5 cut(s) 265, 724, 1018, 1084, 1218
HpyCH4IV ACGT 1 cut(s) 673
HpyCH4V TGCA 5 cut(s) 699, 936, 953, 1211, 1225
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 967
HpyF3I CTNAG 4 cut(s) 327, 520, 1103, 1202
HpySE526I ACGT 1 cut(s) 673
Hsp92II CATG 7 cut(s) 283, 346, 379, 433, 572, 619, 699
Ksp22I TGATCA 1 cut(s) 901
Kzo9I GATC 4 cut(s) 7, 523, 775, 901
LguI GCTCTTC 1 cut(s) 657
LmnI GCTCC 1 cut(s) 466
Lsp1109I GCAGC 1 cut(s) 1185
LweI GCATC 1 cut(s) 708
MaeI CTAG 2 cut(s) 1038, 1255
MaeII ACGT 1 cut(s) 673
MaeIII GTNAC 4 cut(s) 265, 1018, 1078, 1112
MalI GATC 4 cut(s) 9, 525, 777, 903
MboI GATC 4 cut(s) 7, 523, 775, 901
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 3 cut(s) 492, 1073, 1145
MluCI AATT 9 cut(s) 246, 302, 356, 579, 639, 712, 1003, 1183, 1226
MlyI GAGTC 1 cut(s) 140
Mph1103I ATGCAT 1 cut(s) 701
MroXI GAANNNNTTC 1 cut(s) 479
MseI TTAA 1 cut(s) 1220
MspI CCGG 1 cut(s) 1168
MspR9I CCNGG 2 cut(s) 1024, 1168
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 1 cut(s) 1024
MvnI CGCG 1 cut(s) 1067
MwoI GCNNNNNNNGC 2 cut(s) 377, 967
NciI CCSGG 1 cut(s) 1168
NcoI CCATGG 1 cut(s) 375
NdeI CATATG 1 cut(s) 240
NdeII GATC 4 cut(s) 7, 523, 775, 901
NlaIII CATG 7 cut(s) 283, 346, 379, 433, 572, 619, 699
NlaIV GGNNCC 4 cut(s) 381, 447, 462, 491
NmeAIII GCCGAG 1 cut(s) 652
NmuCI GTSAC 3 cut(s) 265, 1018, 1078
NsiI ATGCAT 1 cut(s) 701
PciSI GCTCTTC 1 cut(s) 657
PcsI WCGNNNNNNNCGW 1 cut(s) 345
PctI GAATGC 1 cut(s) 652
PdmI GAANNNNTTC 1 cut(s) 479
PfeI GAWTC 3 cut(s) 117, 539, 692
PflFI GACNNNGTC 1 cut(s) 263
PkrI GCNGC 3 cut(s) 324, 635, 1200
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PpuMI RGGWCCY 1 cut(s) 446
PsiI TTATAA 1 cut(s) 534
Psp1406I AACGTT 1 cut(s) 673
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 1022
PspGI CCWGG 1 cut(s) 1022
PspN4I GGNNCC 4 cut(s) 381, 447, 462, 491
PspPI GGNCC 4 cut(s) 379, 446, 835, 1046
PspPPI RGGWCCY 1 cut(s) 446
PsuI RGATCY 1 cut(s) 523
PsyI GACNNNGTC 1 cut(s) 263
RsaI GTAC 4 cut(s) 123, 762, 1064, 1253
RsaNI GTAC 4 cut(s) 122, 761, 1063, 1252
SapI GCTCTTC 1 cut(s) 657
SaqAI TTAA 1 cut(s) 1220
SatI GCNGC 3 cut(s) 323, 634, 1199
Sau3AI GATC 4 cut(s) 7, 523, 775, 901
Sau96I GGNCC 4 cut(s) 379, 446, 835, 1046
ScaI AGTACT 1 cut(s) 1253
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 1024, 1168
SduI GDGCHC 3 cut(s) 492, 1073, 1145
SfaNI GCATC 1 cut(s) 708
SinI GGWCC 3 cut(s) 446, 835, 1046
Sse9I AATT 9 cut(s) 246, 302, 356, 579, 639, 712, 1003, 1183, 1226
SsiI CCGC 4 cut(s) 323, 634, 783, 1044
SspI AATATT 1 cut(s) 232
SspMI CTAG 2 cut(s) 1038, 1255
StyD4I CCNGG 2 cut(s) 1022, 1166
StyI CCWWGG 3 cut(s) 375, 484, 493
TaaI ACNGT 5 cut(s) 265, 724, 1018, 1084, 1218
TaiI ACGT 1 cut(s) 676
TaqI TCGA 2 cut(s) 657, 1059
TaqII GACCGA 1 cut(s) 299
TasI AATT 9 cut(s) 246, 302, 356, 579, 639, 712, 1003, 1183, 1226
TatI WGTACW 2 cut(s) 760, 1251
TauI GCSGC 2 cut(s) 325, 636
TfiI GAWTC 3 cut(s) 117, 539, 692
Tru1I TTAA 1 cut(s) 1220
Tru9I TTAA 1 cut(s) 1220
TscAI CASTG 3 cut(s) 845, 863, 1221
TseFI GTSAC 3 cut(s) 265, 1018, 1078
TseI GCWGC 1 cut(s) 1198
Tsp45I GTSAC 3 cut(s) 265, 1018, 1078
TspDTI ATGAA 9 cut(s) 17, 195, 281, 418, 443, 552, 557, 822, 995
TspGWI ACGGA 3 cut(s) 204, 390, 516
TspRI CASTG 3 cut(s) 845, 863, 1221
Tth111I GACNNNGTC 1 cut(s) 263
VpaK11BI GGWCC 3 cut(s) 446, 835, 1046
XapI RAATTY 3 cut(s) 579, 1003, 1183
XmiI GTMKAC 2 cut(s) 160, 726
XmnI GAANNNNTTC 1 cut(s) 479
XspI CTAG 2 cut(s) 1038, 1255
ZrmI AGTACT 1 cut(s) 1253
Zsp2I ATGCAT 1 cut(s) 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.