MD13G1038500.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001447
Physical Location & Seq
Reverse (-)
2618831 .. 2619686
856 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1038500.v1.1.491

Sequence Viewer

Length: 687 bp
ATGCATCCTGAAAGTCCGATGTTTCATGAGCGAAGAATTCCTAGTCAGGAAGTAGAATGGGGGAAGGTGAGCTTGATTGAGGCTGTGAGCCGCCTATTGGCGAATGCTCTTCTCGACATCTCAAACCAACATTTTGTTCTCCTCTCAGAGACATGCATTCCCCTCTACAACTTTTCCACTGTCTACTCCTACCTCATCAACTCTACCCGAACTTTCGTAGAGGTCTTCGATGACCCAAGTTCGGTCCGGTACTATCTCAGCAACCATACGAGAATCACGGTAGATCAATGGAGAAAAGGGTGGGCGTGGTTTGCAATTGATAGAGAGGTTGCCATTGAATCCATCTCGGATCGAAAATACTTCCCGGAATTCCTCAAGTGCAAAGGCTATTGCTTTCCGGATGAACATTATTTGCCAACATTTGTGCACTTGAAATTTGCAGCAAGGAATGCAAATAGGACTTTGACCTGGGTTGACTGGAGCAAGGGCGGCCCACACCCGATGGAGTACAGGAGCCCGAACGTGACGGCGGCGCTTCTGAGTAGCCTGAGGAGTGGTTATGGTCGGAAATGTGAATACAATGGAAGGAGCACGGATGTTTGTTTCTTATTCGCCAGAAAGTTCATGCCTGATACTTTGGATAACCTGCTGAGACTTGCACCGCAGATCATGTATTTCAGCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

229

Amino Acids

26.65

Weight (kDa)

8.24

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 5 - 183 1.9e-53 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 654
AccI GTMKAC 1 cut(s) 183
AccIII TCCGGA 1 cut(s) 397
AciI CCGC 4 cut(s) 91, 489, 530, 662
AclWI GGATC 1 cut(s) 357
AcsI RAATTY 3 cut(s) 36, 368, 434
AfaI GTAC 2 cut(s) 251, 509
AfiI CCNNNNNNNGG 2 cut(s) 97, 241
AgsI TTSAA 2 cut(s) 338, 433
AjnI CCWGG 1 cut(s) 467
AjuI GAANNNNNNNTTGG 2 cut(s) 120, 152
AluBI AGCT 1 cut(s) 72
AluI AGCT 1 cut(s) 72
Alw21I GWGCWC 2 cut(s) 429, 593
Alw26I GTCTC 2 cut(s) 143, 646
Alw44I GTGCAC 1 cut(s) 425
AlwI GGATC 1 cut(s) 357
Aor13HI TCCGGA 1 cut(s) 397
AoxI GGCC 1 cut(s) 490
ApaLI GTGCAC 1 cut(s) 425
ApeKI GCWGC 1 cut(s) 440
ApoI RAATTY 3 cut(s) 36, 368, 434
AspLEI GCGC 1 cut(s) 535
AspS9I GGNCC 2 cut(s) 244, 491
AsuC2I CCSGG 1 cut(s) 365
AsuHPI GGTGA 1 cut(s) 79
AvaII GGWCC 1 cut(s) 244
AxyI CCTNAGG 1 cut(s) 548
BaeGI GKGCMC 1 cut(s) 429
BanII GRGCYC 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 217
Bbv12I GWGCWC 2 cut(s) 429, 593
BbvI GCAGC 1 cut(s) 452
BccI CCATC 2 cut(s) 350, 496
BceAI ACGGC 1 cut(s) 543
BciT130I CCWGG 1 cut(s) 469
BcnI CCSGG 1 cut(s) 365
BcoDI GTCTC 2 cut(s) 143, 646
BfaI CTAG 1 cut(s) 42
BfoI RGCGCY 1 cut(s) 536
BfuAI ACCTGC 1 cut(s) 654
BisI GCNGC 4 cut(s) 91, 441, 490, 531
BlsI GCNGC 4 cut(s) 92, 442, 491, 532
Bme1390I CCNGG 2 cut(s) 365, 469
Bme18I GGWCC 1 cut(s) 244
BmgT120I GGNCC 2 cut(s) 244, 491
BmiI GGNNCC 1 cut(s) 515
BmrFI CCNGG 2 cut(s) 365, 469
BmsI GCATC 1 cut(s) 13
BpiI GAAGAC 1 cut(s) 217
BpmI CTGGAG 1 cut(s) 499
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 1 cut(s) 365
BsaJI CCNNGG 1 cut(s) 468
BsaWI WCCGGW 2 cut(s) 246, 397
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 241
Bse1I ACTGG 1 cut(s) 482
Bse21I CCTNAGG 1 cut(s) 548
BseAI TCCGGA 1 cut(s) 397
BseBI CCWGG 1 cut(s) 469
BseDI CCNNGG 1 cut(s) 468
BseGI GGATG 3 cut(s) 4, 406, 601
BseLI CCNNNNNNNGG 2 cut(s) 97, 241
BseMII CTCAG 5 cut(s) 159, 271, 530, 539, 641
BseNI ACTGG 1 cut(s) 482
BseRI GAGGAG 2 cut(s) 131, 565
BseSI GKGCMC 1 cut(s) 429
BseXI GCAGC 1 cut(s) 452
BshFI GGCC 1 cut(s) 492
BsiHKAI GWGCWC 2 cut(s) 429, 593
BsiSI CCGG 3 cut(s) 247, 365, 398
BslI CCNNNNNNNGG 2 cut(s) 97, 241
BsmAI GTCTC 2 cut(s) 143, 646
BsmI GAATGC 3 cut(s) 109, 156, 454
BsnI GGCC 1 cut(s) 492
Bsp1286I GDGCHC 3 cut(s) 429, 518, 593
Bsp13I TCCGGA 1 cut(s) 397
Bsp143I GATC 3 cut(s) 283, 349, 666
BspACI CCGC 4 cut(s) 91, 489, 530, 662
BspANI GGCC 1 cut(s) 492
BspCNI CTCAG 5 cut(s) 158, 270, 531, 540, 642
BspEI TCCGGA 1 cut(s) 397
BspHI TCATGA 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 515
BspMI ACCTGC 1 cut(s) 654
BspPI GGATC 1 cut(s) 357
BspQI GCTCTTC 1 cut(s) 114
BsrI ACTGG 1 cut(s) 482
BssECI CCNNGG 1 cut(s) 468
BssMI GATC 3 cut(s) 283, 349, 666
Bst2UI CCWGG 1 cut(s) 469
Bst4CI ACNGT 2 cut(s) 181, 280
Bst6I CTCTTC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 449
BstDEI CTNAG 5 cut(s) 145, 257, 539, 548, 650
BstF5I GGATG 3 cut(s) 4, 406, 601
BstH2I RGCGCY 1 cut(s) 536
BstHHI GCGC 1 cut(s) 535
BstKTI GATC 3 cut(s) 286, 352, 669
BstMAI GTCTC 2 cut(s) 143, 646
BstMBI GATC 3 cut(s) 283, 349, 666
BstMWI GCNNNNNNNGC 3 cut(s) 311, 449, 489
BstNI CCWGG 1 cut(s) 469
BstNSI RCATGY 1 cut(s) 156
BstSCI CCNGG 2 cut(s) 363, 467
BstSLI GKGCMC 1 cut(s) 429
BstV1I GCAGC 1 cut(s) 452
BstV2I GAAGAC 1 cut(s) 217
Bsu36I CCTNAGG 1 cut(s) 548
BsuRI GGCC 1 cut(s) 492
BtsCI GGATG 3 cut(s) 4, 406, 601
BtsIMutI CAGTG 1 cut(s) 177
BveI ACCTGC 1 cut(s) 654
CciI TCATGA 1 cut(s) 25
CfoI GCGC 1 cut(s) 535
Cfr13I GGNCC 2 cut(s) 244, 491
CpoI CGGWCCG 1 cut(s) 244
Csp6I GTAC 2 cut(s) 250, 508
CspI CGGWCCG 1 cut(s) 244
CviAII CATG 4 cut(s) 26, 153, 625, 670
CviJI RGCY 7 cut(s) 72, 83, 90, 387, 492, 516, 546
CviKI_1 RGCY 7 cut(s) 72, 83, 90, 387, 492, 516, 546
CviQI GTAC 2 cut(s) 250, 508
DdeI CTNAG 5 cut(s) 145, 257, 539, 548, 650
DpnI GATC 3 cut(s) 285, 351, 668
DpnII GATC 3 cut(s) 283, 349, 666
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
Eco24I GRGCYC 1 cut(s) 518
Eco47I GGWCC 1 cut(s) 244
Eco81I CCTNAGG 1 cut(s) 548
EcoRI GAATTC 2 cut(s) 36, 368
EcoRII CCWGG 1 cut(s) 467
EcoT22I ATGCAT 2 cut(s) 6, 158
EcoT38I GRGCYC 1 cut(s) 518
FaeI CATG 4 cut(s) 29, 156, 628, 673
FaiI YATR 8 cut(s) 27, 154, 267, 561, 626, 671, 683, 685
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FatI CATG 4 cut(s) 25, 152, 624, 669
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 4 cut(s) 91, 441, 490, 531
FokI GGATG 2 cut(s) 413, 608
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 4 cut(s) 91, 441, 490, 531
FspBI CTAG 1 cut(s) 42
GlaI GCGC 1 cut(s) 534
GluI GCNGC 4 cut(s) 91, 441, 490, 531
GsuI CTGGAG 1 cut(s) 499
HaeII RGCGCY 1 cut(s) 536
HaeIII GGCC 1 cut(s) 492
HapII CCGG 3 cut(s) 247, 365, 398
HhaI GCGC 1 cut(s) 535
Hin1II CATG 4 cut(s) 29, 156, 628, 673
Hin6I GCGC 1 cut(s) 533
HinP1I GCGC 1 cut(s) 533
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HinfI GANTC 2 cut(s) 273, 338
HpaII CCGG 3 cut(s) 247, 365, 398
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 3 cut(s) 184, 427, 475
Hpy188I TCNGA 5 cut(s) 18, 148, 349, 540, 567
Hpy188III TCNNGA 5 cut(s) 8, 26, 47, 113, 398
Hpy8I GTNNAC 3 cut(s) 184, 427, 475
HpyAV CCTTC 2 cut(s) 58, 579
HpyCH4III ACNGT 2 cut(s) 181, 280
HpyCH4IV ACGT 1 cut(s) 522
HpyCH4V TGCA 8 cut(s) 4, 156, 314, 381, 427, 440, 452, 659
HpyF10VI GCNNNNNNNGC 3 cut(s) 311, 449, 489
HpyF3I CTNAG 5 cut(s) 145, 257, 539, 548, 650
HpySE526I ACGT 1 cut(s) 522
Hsp92II CATG 4 cut(s) 29, 156, 628, 673
HspAI GCGC 1 cut(s) 533
Kpn2I TCCGGA 1 cut(s) 397
Kzo9I GATC 3 cut(s) 283, 349, 666
LguI GCTCTTC 1 cut(s) 114
LmnI GCTCC 3 cut(s) 480, 513, 588
Lsp1109I GCAGC 1 cut(s) 452
LweI GCATC 1 cut(s) 13
MaeI CTAG 1 cut(s) 42
MaeII ACGT 1 cut(s) 522
MaeIII GTNAC 1 cut(s) 523
MalI GATC 3 cut(s) 285, 351, 668
MboI GATC 3 cut(s) 283, 349, 666
MboII GAAGA 3 cut(s) 45, 101, 217
MfeI CAATTG 1 cut(s) 315
MhlI GDGCHC 3 cut(s) 429, 518, 593
MluCI AATT 4 cut(s) 36, 315, 368, 434
MmeI TCCRAC 1 cut(s) 545
MnlI CCTC 8 cut(s) 73, 152, 173, 203, 214, 319, 383, 543
Mph1103I ATGCAT 2 cut(s) 6, 158
MroI TCCGGA 1 cut(s) 397
MspI CCGG 3 cut(s) 247, 365, 398
MspR9I CCNGG 2 cut(s) 365, 469
MunI CAATTG 1 cut(s) 315
Mva1269I GAATGC 3 cut(s) 109, 156, 454
MvaI CCWGG 1 cut(s) 469
MwoI GCNNNNNNNGC 3 cut(s) 311, 449, 489
NciI CCSGG 1 cut(s) 365
NdeII GATC 3 cut(s) 283, 349, 666
NlaIII CATG 4 cut(s) 29, 156, 628, 673
NlaIV GGNNCC 1 cut(s) 515
NmuCI GTSAC 1 cut(s) 523
NsiI ATGCAT 2 cut(s) 6, 158
NspI RCATGY 1 cut(s) 156
PagI TCATGA 1 cut(s) 25
PciSI GCTCTTC 1 cut(s) 114
PctI GAATGC 3 cut(s) 109, 156, 454
PfeI GAWTC 2 cut(s) 273, 338
PfoI TCCNGGA 1 cut(s) 363
PkrI GCNGC 4 cut(s) 92, 442, 491, 532
Psp6I CCWGG 1 cut(s) 467
PspGI CCWGG 1 cut(s) 467
PspN4I GGNNCC 1 cut(s) 515
PspPI GGNCC 2 cut(s) 244, 491
RsaI GTAC 2 cut(s) 251, 509
RsaNI GTAC 2 cut(s) 250, 508
Rsr2I CGGWCCG 1 cut(s) 244
RsrII CGGWCCG 1 cut(s) 244
SapI GCTCTTC 1 cut(s) 114
SatI GCNGC 4 cut(s) 91, 441, 490, 531
Sau3AI GATC 3 cut(s) 283, 349, 666
Sau96I GGNCC 2 cut(s) 244, 491
ScrFI CCNGG 2 cut(s) 365, 469
SduI GDGCHC 3 cut(s) 429, 518, 593
SetI ASST 8 cut(s) 69, 74, 195, 225, 330, 470, 525, 648
SfaNI GCATC 1 cut(s) 13
SinI GGWCC 1 cut(s) 244
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 4 cut(s) 36, 315, 368, 434
SsiI CCGC 4 cut(s) 91, 489, 530, 662
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 2 cut(s) 363, 467
TaaI ACNGT 2 cut(s) 181, 280
TaiI ACGT 1 cut(s) 525
TaqI TCGA 3 cut(s) 114, 228, 352
TaqII GACCGA 1 cut(s) 232
TasI AATT 4 cut(s) 36, 315, 368, 434
TatI WGTACW 1 cut(s) 507
TauI GCSGC 3 cut(s) 93, 492, 533
TfiI GAWTC 2 cut(s) 273, 338
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 1 cut(s) 523
TseI GCWGC 1 cut(s) 440
Tsp45I GTSAC 1 cut(s) 523
TspDTI ATGAA 3 cut(s) 14, 417, 613
TspGWI ACGGA 1 cut(s) 608
TspRI CASTG 1 cut(s) 184
VneI GTGCAC 1 cut(s) 425
VpaK11BI GGWCC 1 cut(s) 244
XapI RAATTY 3 cut(s) 36, 368, 434
XceI RCATGY 1 cut(s) 156
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 1 cut(s) 42
Zsp2I ATGCAT 2 cut(s) 6, 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.