MD13G1038800.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001447
Physical Location & Seq
Reverse (-)
2627333 .. 2629195
1863 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1038800.v1.1.491

Sequence Viewer

Length: 1206 bp
ATGAAGGGACCAAATCCAAACCCTTCAAACTTCTTCTCAAAGCTCTTCAATGTCCAGCTCCACCTCCTCAATCTCCTCTTCTACTTCCTCGTCTTCGGCGGCGGCCTGACCCTCGGAATGGTGCTCAGCTTCTACATGAAAGACTTCTCCTTCAGTCTGCAATTAGCACAGTTCTCCTTCTTCTCAACCTCAACATCTGCATCCAATCCGCACAACCTAATGCCGAACGTTAAAGCAGTTGAACCCAACACGAGTCACCGTCCTCCTCACGTAGGATTGAAGGAATATCTAAAGCCGCCGGAGGTTTTCCACGACATGGACGAAGTGGAGTTGCTGTGGAGAGCTTCGATGAGTCCTCGGATTTCCGAGTATCCGTTTCCCCGTGTTCCCAAGGTTGCTTTCTTGTTCTTGGTAAAGGGTCCTGTTCTTTTGGCCCCGTTGTGGGAGAAGTTCTTCAAAGGACATAAAGGATTGTACTCGATTTACGTGCATTCGAATCCATCTTACAATGGATCGCACCCGGAAAGTCCAGTTTTTCATGGCAGAAGAATTCCTAGCCAGGAAGTAGAATGGGGAAATGTGAACATGATTGAGGCCGAGCGCCGCCTATTGGCGAATGCTCTTCTCGACATCTCAAACCAACGGTTCATTCTGCTCTCAGAGTCATGCATTCCCCTCCACAACTTCTCCACTGTCTACAATTACCTCGTCCGCTCCAATGAAACTTTTGTGGGAGTCTATGATGATCCAAGCTCGGTCGGGCGTGGCAGGTACAATTCTCACATGTATCCACAAATCTCGTTGAGTCAATGGCGAAAAGGGTCACAATGGTTTGAAATTGACAGAAGCCTTGCCATTGAAGTTGTCTCGGATAAAAAATACTTCCCGGTGTTCCAAACTTACTGCAGGGGCTCATGCTACGCAGATGAGCATTACTTGCCAACATATGTGAACATAAATTTTGCACAGAAGAATTCAAATAGGAGTTTGACCTGGGTTGACTGGTCGAGGGGCGGCCCACATCCAGCCAAGTTTACTAGAACGGATGTGACGGTTAAGGCTTTGAATAGCTTGAGGAATCGGACTGCATGTATATATAATGGAAGAGTTTCCAATGTTTGTTTCTTGTTTGCTAGAAAGTTTGGGCCTGGTACACTGGATAGACTGCTGAGGTTTGCGCCAAAGCTCATGCGATTCAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

402

Amino Acids

46.17

Weight (kDa)

9.62

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 132 - 358 9.7e-80 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 759
AccB7I CCANNNNNTGG 1 cut(s) 316
AccBSI CCGCTC 1 cut(s) 714
AccI GTMKAC 1 cut(s) 696
AciI CCGC 7 cut(s) 99, 102, 209, 296, 604, 712, 1014
AclI AACGTT 1 cut(s) 228
AclWI GGATC 2 cut(s) 520, 740
AcsI RAATTY 3 cut(s) 549, 958, 973
AcuI CTGAAG 1 cut(s) 136
AfaI GTAC 3 cut(s) 476, 773, 1153
AfiI CCNNNNNNNGG 6 cut(s) 118, 272, 316, 441, 442, 610
AflIII ACRYGT 1 cut(s) 783
AjnI CCWGG 3 cut(s) 558, 992, 1147
AjuI GAANNNNNNNTTGG 6 cut(s) 383, 415, 633, 665, 1106, 1138
AluBI AGCT 7 cut(s) 43, 58, 129, 344, 753, 1071, 1186
AluI AGCT 7 cut(s) 43, 58, 129, 344, 753, 1071, 1186
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 1 cut(s) 871
AlwI GGATC 2 cut(s) 520, 740
AoxI GGCC 5 cut(s) 103, 432, 594, 1015, 1145
ApoI RAATTY 3 cut(s) 549, 958, 973
Asp700I GAANNNNTTC 3 cut(s) 143, 452, 1108
AspLEI GCGC 2 cut(s) 603, 1180
AspS9I GGNCC 5 cut(s) 8, 419, 433, 1016, 1145
AsuC2I CCSGG 2 cut(s) 521, 887
AsuHPI GGTGA 1 cut(s) 248
AsuII TTCGAA 1 cut(s) 494
AvaII GGWCC 2 cut(s) 8, 419
BanII GRGCYC 1 cut(s) 914
BauI CACGAG 1 cut(s) 250
BbsI GAAGAC 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 126
BbvCI CCTCAGC 1 cut(s) 1169
BccI CCATC 1 cut(s) 508
BcgI CGANNNNNNTGC 2 cut(s) 469, 503
BciT130I CCWGG 3 cut(s) 560, 994, 1149
BciVI GTATCC 2 cut(s) 381, 798
BcnI CCSGG 2 cut(s) 521, 887
BcoDI GTCTC 1 cut(s) 871
BfaI CTAG 3 cut(s) 555, 1038, 1134
BfmI CTRYAG 1 cut(s) 904
BfoI RGCGCY 1 cut(s) 604
BfuAI ACCTGC 1 cut(s) 759
BfuI GTATCC 2 cut(s) 381, 798
BisI GCNGC 5 cut(s) 100, 103, 296, 604, 1015
BlpI GCTNAGC 1 cut(s) 125
BlsI GCNGC 5 cut(s) 101, 104, 297, 605, 1016
Bme1390I CCNGG 5 cut(s) 521, 560, 887, 994, 1149
Bme18I GGWCC 2 cut(s) 8, 419
BmgT120I GGNCC 5 cut(s) 8, 419, 433, 1016, 1145
BmiI GGNNCC 3 cut(s) 9, 420, 435
BmrFI CCNGG 5 cut(s) 521, 560, 887, 994, 1149
BmsI GCATC 1 cut(s) 209
BpiI GAAGAC 1 cut(s) 85
Bpu10I CCTNAGC 1 cut(s) 1169
Bpu1102I GCTNAGC 1 cut(s) 125
Bpu14I TTCGAA 1 cut(s) 494
BpuEI CTTGAG 1 cut(s) 1093
BpuMI CCSGG 2 cut(s) 521, 887
BsaAI YACGTR 2 cut(s) 271, 487
BsaJI CCNNGG 4 cut(s) 112, 356, 390, 993
BsaXI ACNNNNNCTCC 4 cut(s) 320, 350, 671, 701
Bsc4I CCNNNNNNNGG 6 cut(s) 118, 272, 316, 441, 442, 610
Bse1I ACTGG 3 cut(s) 530, 1007, 1161
BseBI CCWGG 3 cut(s) 560, 994, 1149
BseDI CCNNGG 4 cut(s) 112, 356, 390, 993
BseGI GGATG 3 cut(s) 200, 1021, 1051
BseLI CCNNNNNNNGG 6 cut(s) 118, 272, 316, 441, 442, 610
BseMII CTCAG 3 cut(s) 139, 672, 1160
BseNI ACTGG 3 cut(s) 530, 1007, 1161
BseRI GAGGAG 3 cut(s) 56, 65, 255
Bsh1285I CGRYCG 1 cut(s) 759
BshFI GGCC 5 cut(s) 105, 434, 596, 1017, 1147
BsiEI CGRYCG 1 cut(s) 759
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 3 cut(s) 299, 521, 887
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 6 cut(s) 118, 272, 316, 441, 442, 610
BsmAI GTCTC 1 cut(s) 871
BsmFI GGGAC 1 cut(s) 21
BsmI GAATGC 3 cut(s) 490, 622, 669
BsnI GGCC 5 cut(s) 105, 434, 596, 1017, 1147
Bsp119I TTCGAA 1 cut(s) 494
Bsp1286I GDGCHC 2 cut(s) 126, 914
Bsp143I GATC 2 cut(s) 512, 745
Bsp1720I GCTNAGC 1 cut(s) 125
BspACI CCGC 7 cut(s) 99, 102, 209, 296, 604, 712, 1014
BspANI GGCC 5 cut(s) 105, 434, 596, 1017, 1147
BspCNI CTCAG 3 cut(s) 138, 671, 1161
BspLI GGNNCC 3 cut(s) 9, 420, 435
BspMAI CTGCAG 1 cut(s) 908
BspMI ACCTGC 1 cut(s) 759
BspPI GGATC 2 cut(s) 520, 740
BspQI GCTCTTC 2 cut(s) 50, 627
BspT104I TTCGAA 1 cut(s) 494
BsrBI CCGCTC 1 cut(s) 714
BsrI ACTGG 3 cut(s) 530, 1007, 1161
BssECI CCNNGG 4 cut(s) 112, 356, 390, 993
BssMI GATC 2 cut(s) 512, 745
BssSI CACGAG 1 cut(s) 250
BssT1I CCWWGG 1 cut(s) 390
Bst2BI CACGAG 1 cut(s) 250
Bst2UI CCWGG 3 cut(s) 560, 994, 1149
Bst4CI ACNGT 5 cut(s) 171, 260, 645, 694, 1054
Bst6I CTCTTC 4 cut(s) 50, 83, 627, 1099
BstAPI GCANNNNNTGC 1 cut(s) 937
BstBAI YACGTR 2 cut(s) 271, 487
BstBI TTCGAA 1 cut(s) 494
BstDEI CTNAG 3 cut(s) 125, 658, 1169
BstENI CCTNNNNNAGG 1 cut(s) 270
BstF5I GGATG 3 cut(s) 200, 1021, 1051
BstH2I RGCGCY 1 cut(s) 604
BstHHI GCGC 2 cut(s) 603, 1180
BstKTI GATC 2 cut(s) 515, 748
BstMAI GTCTC 1 cut(s) 871
BstMBI GATC 2 cut(s) 512, 745
BstMCI CGRYCG 1 cut(s) 759
BstMWI GCNNNNNNNGC 1 cut(s) 937
BstNI CCWGG 3 cut(s) 560, 994, 1149
BstNSI RCATGY 2 cut(s) 787, 1092
BstSCI CCNGG 5 cut(s) 519, 558, 885, 992, 1147
BstSFI CTRYAG 1 cut(s) 904
BstV2I GAAGAC 1 cut(s) 85
BsuI GTATCC 2 cut(s) 381, 798
BsuRI GGCC 5 cut(s) 105, 434, 596, 1017, 1147
BtsCI GGATG 3 cut(s) 200, 1021, 1051
BtsIMutI CAGTG 2 cut(s) 690, 1154
BveI ACCTGC 1 cut(s) 759
CfoI GCGC 2 cut(s) 603, 1180
Cfr13I GGNCC 5 cut(s) 8, 419, 433, 1016, 1145
Csp6I GTAC 3 cut(s) 475, 772, 1152
CviAII CATG 9 cut(s) 136, 316, 539, 586, 666, 784, 915, 1089, 1189
CviQI GTAC 3 cut(s) 475, 772, 1152
DdeI CTNAG 3 cut(s) 125, 658, 1169
DpnI GATC 2 cut(s) 514, 747
DpnII GATC 2 cut(s) 512, 745
Eam1104I CTCTTC 4 cut(s) 50, 83, 627, 1099
EarI CTCTTC 4 cut(s) 50, 83, 627, 1099
Eco130I CCWWGG 1 cut(s) 390
Eco24I GRGCYC 1 cut(s) 914
Eco47I GGWCC 2 cut(s) 8, 419
Eco57I CTGAAG 1 cut(s) 136
EcoNI CCTNNNNNAGG 1 cut(s) 270
EcoO109I RGGNCCY 1 cut(s) 419
EcoRI GAATTC 2 cut(s) 549, 973
EcoRII CCWGG 3 cut(s) 558, 992, 1147
EcoT14I CCWWGG 1 cut(s) 390
EcoT22I ATGCAT 1 cut(s) 671
EcoT38I GRGCYC 1 cut(s) 914
ErhI CCWWGG 1 cut(s) 390
FaeI CATG 9 cut(s) 139, 319, 542, 589, 669, 787, 918, 1092, 1192
FaqI GGGAC 1 cut(s) 21
FatI CATG 9 cut(s) 135, 315, 538, 585, 665, 783, 914, 1088, 1188
FauNDI CATATG 1 cut(s) 946
FblI GTMKAC 1 cut(s) 696
Fnu4HI GCNGC 5 cut(s) 100, 103, 296, 604, 1015
FokI GGATG 3 cut(s) 187, 1008, 1058
FriOI GRGCYC 1 cut(s) 914
Fsp4HI GCNGC 5 cut(s) 100, 103, 296, 604, 1015
FspBI CTAG 3 cut(s) 555, 1038, 1134
GlaI GCGC 2 cut(s) 602, 1179
GluI GCNGC 5 cut(s) 100, 103, 296, 604, 1015
HaeII RGCGCY 1 cut(s) 604
HaeIII GGCC 5 cut(s) 105, 434, 596, 1017, 1147
HapII CCGG 3 cut(s) 299, 521, 887
HhaI GCGC 2 cut(s) 603, 1180
Hin1II CATG 9 cut(s) 139, 319, 542, 589, 669, 787, 918, 1092, 1192
Hin6I GCGC 2 cut(s) 601, 1178
HinP1I GCGC 2 cut(s) 601, 1178
HincII GTYRAC 1 cut(s) 1000
HindII GTYRAC 1 cut(s) 1000
HinfI GANTC 8 cut(s) 253, 352, 496, 662, 735, 805, 1078, 1194
HpaII CCGG 3 cut(s) 299, 521, 887
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 6 cut(s) 583, 697, 952, 1000, 1035, 1154
Hpy188I TCNGA 6 cut(s) 116, 360, 367, 661, 871, 1083
Hpy188III TCNNGA 1 cut(s) 626
Hpy8I GTNNAC 6 cut(s) 583, 697, 952, 1000, 1035, 1154
HpyAV CCTTC 4 cut(s) 33, 160, 187, 274
HpyCH4III ACNGT 5 cut(s) 171, 260, 645, 694, 1054
HpyCH4IV ACGT 3 cut(s) 228, 270, 486
HpyCH4V TGCA 7 cut(s) 160, 200, 490, 669, 906, 965, 1088
HpyF10VI GCNNNNNNNGC 1 cut(s) 937
HpyF3I CTNAG 3 cut(s) 125, 658, 1169
HpySE526I ACGT 3 cut(s) 228, 270, 486
Hsp92II CATG 9 cut(s) 139, 319, 542, 589, 669, 787, 918, 1092, 1192
HspAI GCGC 2 cut(s) 601, 1178
Kzo9I GATC 2 cut(s) 512, 745
LguI GCTCTTC 2 cut(s) 50, 627
LmnI GCTCC 2 cut(s) 63, 719
LweI GCATC 1 cut(s) 209
MaeI CTAG 3 cut(s) 555, 1038, 1134
MaeII ACGT 3 cut(s) 228, 270, 486
MaeIII GTNAC 3 cut(s) 254, 822, 1048
MalI GATC 2 cut(s) 514, 747
MbiI CCGCTC 1 cut(s) 714
MboI GATC 2 cut(s) 512, 745
MhlI GDGCHC 2 cut(s) 126, 914
MluCI AATT 7 cut(s) 161, 549, 700, 775, 837, 958, 973
MlyI GAGTC 5 cut(s) 262, 361, 671, 744, 814
Mph1103I ATGCAT 1 cut(s) 671
MroXI GAANNNNTTC 3 cut(s) 143, 452, 1108
MseI TTAA 2 cut(s) 231, 1056
MspI CCGG 3 cut(s) 299, 521, 887
MspR9I CCNGG 5 cut(s) 521, 560, 887, 994, 1149
Mva1269I GAATGC 3 cut(s) 490, 622, 669
MvaI CCWGG 3 cut(s) 560, 994, 1149
MwoI GCNNNNNNNGC 1 cut(s) 937
NciI CCSGG 2 cut(s) 521, 887
NdeI CATATG 1 cut(s) 946
NdeII GATC 2 cut(s) 512, 745
NlaIII CATG 9 cut(s) 139, 319, 542, 589, 669, 787, 918, 1092, 1192
NlaIV GGNNCC 3 cut(s) 9, 420, 435
NmeAIII GCCGAG 1 cut(s) 622
NmuCI GTSAC 3 cut(s) 254, 822, 1048
NsiI ATGCAT 1 cut(s) 671
NspI RCATGY 2 cut(s) 787, 1092
NspV TTCGAA 1 cut(s) 494
PciI ACATGT 1 cut(s) 783
PciSI GCTCTTC 2 cut(s) 50, 627
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PctI GAATGC 3 cut(s) 490, 622, 669
PdmI GAANNNNTTC 3 cut(s) 143, 452, 1108
PfeI GAWTC 3 cut(s) 496, 1078, 1194
PflMI CCANNNNNTGG 1 cut(s) 316
PkrI GCNGC 5 cut(s) 101, 104, 297, 605, 1016
PleI GAGTC 5 cut(s) 261, 360, 670, 743, 813
PpsI GAGTC 5 cut(s) 261, 360, 670, 743, 813
Ppu21I YACGTR 2 cut(s) 271, 487
PpuMI RGGWCCY 1 cut(s) 419
PscI ACATGT 1 cut(s) 783
Psp1406I AACGTT 1 cut(s) 228
Psp5II RGGWCCY 1 cut(s) 419
Psp6I CCWGG 3 cut(s) 558, 992, 1147
PspGI CCWGG 3 cut(s) 558, 992, 1147
PspN4I GGNNCC 3 cut(s) 9, 420, 435
PspPI GGNCC 5 cut(s) 8, 419, 433, 1016, 1145
PspPPI RGGWCCY 1 cut(s) 419
PstI CTGCAG 1 cut(s) 908
RsaI GTAC 3 cut(s) 476, 773, 1153
RsaNI GTAC 3 cut(s) 475, 772, 1152
SapI GCTCTTC 2 cut(s) 50, 627
SaqAI TTAA 2 cut(s) 231, 1056
SatI GCNGC 5 cut(s) 100, 103, 296, 604, 1015
Sau3AI GATC 2 cut(s) 512, 745
Sau96I GGNCC 5 cut(s) 8, 419, 433, 1016, 1145
SchI GAGTC 5 cut(s) 262, 361, 671, 744, 814
ScrFI CCNGG 5 cut(s) 521, 560, 887, 994, 1149
SduI GDGCHC 2 cut(s) 126, 914
SfaNI GCATC 1 cut(s) 209
SfcI CTRYAG 1 cut(s) 904
SfuI TTCGAA 1 cut(s) 494
SinI GGWCC 2 cut(s) 8, 419
SmlI CTYRAG 1 cut(s) 1072
SmoI CTYRAG 1 cut(s) 1072
Sse9I AATT 7 cut(s) 161, 549, 700, 775, 837, 958, 973
SsiI CCGC 7 cut(s) 99, 102, 209, 296, 604, 712, 1014
SspMI CTAG 3 cut(s) 555, 1038, 1134
StyD4I CCNGG 5 cut(s) 519, 558, 885, 992, 1147
StyI CCWWGG 1 cut(s) 390
TaaI ACNGT 5 cut(s) 171, 260, 645, 694, 1054
TaiI ACGT 3 cut(s) 231, 273, 489
TaqI TCGA 5 cut(s) 347, 479, 494, 627, 1007
TaqII GACCGA 1 cut(s) 745
TasI AATT 7 cut(s) 161, 549, 700, 775, 837, 958, 973
TatI WGTACW 1 cut(s) 474
TauI GCSGC 5 cut(s) 102, 105, 298, 606, 1017
TfiI GAWTC 3 cut(s) 496, 1078, 1194
Tru1I TTAA 2 cut(s) 231, 1056
Tru9I TTAA 2 cut(s) 231, 1056
TscAI CASTG 2 cut(s) 697, 1161
TseFI GTSAC 3 cut(s) 254, 822, 1048
Tsp45I GTSAC 3 cut(s) 254, 822, 1048
TspDTI ATGAA 5 cut(s) 17, 152, 527, 637, 735
TspGWI ACGGA 2 cut(s) 363, 1058
TspRI CASTG 2 cut(s) 697, 1161
Van91I CCANNNNNTGG 1 cut(s) 316
VpaK11BI GGWCC 2 cut(s) 8, 419
XagI CCTNNNNNAGG 1 cut(s) 270
XapI RAATTY 3 cut(s) 549, 958, 973
XceI RCATGY 2 cut(s) 787, 1092
XmiI GTMKAC 1 cut(s) 696
XmnI GAANNNNTTC 3 cut(s) 143, 452, 1108
XspI CTAG 3 cut(s) 555, 1038, 1134
Zsp2I ATGCAT 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.