MD16G1014100.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001447
Physical Location & Seq
Forward (+)
1092405 .. 1093040
636 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1014100.v1.1.491

Sequence Viewer

Length: 636 bp
ATGAAGGTACTAAATCAAAACCCTTTAAACTTCTTCTCAAAGCTCTTCAATTTCCAGCTCAACCTCCTCAATCTCCTCTTCTACTTCCTCCTCTTCGGCGGCGGTCTGACCCTCGGAATGGTGCTCAGCTTCTACATGAAAGACTTTTCCTTGAGTCTGCAATTAGCACAATTCTCCTTCTCGACCTCAACATCTGCATCCAATCCGCACAGCCTAATGCCGAACGTAAAAGCAGTCCAACCGAACGAGAGCTATCGTCCTCCTCGTATAGGATTGAAGGAGTATCTAAAGCCGCCGGAGGTTTTCCACGACATGGACGATGTGGAGTTGCTGTGGAGAGCTTCGATGAGTCCTCGGATTTCCGAGTATCCGTTTCCCCGTGCTCCCAAGGTTGCCTTCTTGTTCTTGGTAAAGGGTCCTGTTCTTTTGGCCCCGTTGTGGGAGAAGTTCTTCAAAGGACATCAAGGATTGTACTCGATCTACGTGCACTCGAATCCATCTTACAATGGATCGCACCCGGAAACTCCAGTTTTTCATGGCAGAAGAATTCCTAGCCAGGTCAGTTACTTTTCCTGCATAAATTTATTTGTTAGCTATCCTGCACACCGTGATCTGATGACGATTTTGGAGTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

212

Amino Acids

24.22

Weight (kDa)

9.21

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 131 - 195 4e-12 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 313
AciI CCGC 4 cut(s) 99, 102, 206, 293
AclWI GGATC 1 cut(s) 517
AcsI RAATTY 2 cut(s) 546, 580
AdeI CACNNNGTG 1 cut(s) 608
AfaI GTAC 2 cut(s) 9, 473
AfiI CCNNNNNNNGG 5 cut(s) 118, 269, 313, 438, 439
AgsI TTSAA 3 cut(s) 49, 277, 454
AjnI CCWGG 1 cut(s) 555
AjuI GAANNNNNNNTTGG 2 cut(s) 380, 412
AluBI AGCT 6 cut(s) 43, 58, 129, 252, 341, 594
AluI AGCT 6 cut(s) 43, 58, 129, 252, 341, 594
Alw21I GWGCWC 3 cut(s) 126, 385, 489
Alw44I GTGCAC 1 cut(s) 485
AlwI GGATC 1 cut(s) 517
AoxI GGCC 1 cut(s) 429
ApaLI GTGCAC 1 cut(s) 485
ApoI RAATTY 2 cut(s) 546, 580
Asp700I GAANNNNTTC 1 cut(s) 449
AspS9I GGNCC 2 cut(s) 416, 430
AsuC2I CCSGG 1 cut(s) 518
AvaII GGWCC 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 489
Bbv12I GWGCWC 3 cut(s) 126, 385, 489
BccI CCATC 1 cut(s) 505
BcgI CGANNNNNNTGC 2 cut(s) 466, 500
BciT130I CCWGG 1 cut(s) 557
BciVI GTATCC 1 cut(s) 378
BcnI CCSGG 1 cut(s) 518
BfaI CTAG 1 cut(s) 552
BfuI GTATCC 1 cut(s) 378
BisI GCNGC 2 cut(s) 100, 293
BlpI GCTNAGC 1 cut(s) 125
BlsI GCNGC 2 cut(s) 101, 294
Bme1390I CCNGG 2 cut(s) 518, 557
Bme18I GGWCC 1 cut(s) 416
BmgT120I GGNCC 2 cut(s) 416, 430
BmiI GGNNCC 2 cut(s) 417, 432
BmrFI CCNGG 2 cut(s) 518, 557
BmsI GCATC 1 cut(s) 206
BpmI CTGGAG 1 cut(s) 510
Bpu1102I GCTNAGC 1 cut(s) 125
BpuEI CTTGAG 1 cut(s) 172
BpuMI CCSGG 1 cut(s) 518
BsaAI YACGTR 1 cut(s) 484
BsaJI CCNNGG 3 cut(s) 112, 353, 387
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
Bsc4I CCNNNNNNNGG 5 cut(s) 118, 269, 313, 438, 439
Bse1I ACTGG 1 cut(s) 527
BseBI CCWGG 1 cut(s) 557
BseDI CCNNGG 3 cut(s) 112, 353, 387
BseGI GGATG 1 cut(s) 197
BseLI CCNNNNNNNGG 5 cut(s) 118, 269, 313, 438, 439
BseMII CTCAG 1 cut(s) 139
BseNI ACTGG 1 cut(s) 527
BseRI GAGGAG 4 cut(s) 56, 65, 80, 252
BseSI GKGCMC 1 cut(s) 489
BsgI GTGCAG 1 cut(s) 585
BshFI GGCC 1 cut(s) 431
BsiHKAI GWGCWC 3 cut(s) 126, 385, 489
BsiSI CCGG 2 cut(s) 296, 518
BslI CCNNNNNNNGG 5 cut(s) 118, 269, 313, 438, 439
BsnI GGCC 1 cut(s) 431
Bsp1286I GDGCHC 3 cut(s) 126, 385, 489
Bsp143I GATC 3 cut(s) 477, 509, 610
Bsp1720I GCTNAGC 1 cut(s) 125
BspACI CCGC 4 cut(s) 99, 102, 206, 293
BspANI GGCC 1 cut(s) 431
BspCNI CTCAG 1 cut(s) 138
BspLI GGNNCC 2 cut(s) 417, 432
BspPI GGATC 1 cut(s) 517
BspQI GCTCTTC 1 cut(s) 50
BsrI ACTGG 1 cut(s) 527
BssECI CCNNGG 3 cut(s) 112, 353, 387
BssMI GATC 3 cut(s) 477, 509, 610
BssT1I CCWWGG 1 cut(s) 387
Bst2UI CCWGG 1 cut(s) 557
Bst4CI ACNGT 1 cut(s) 608
Bst6I CTCTTC 3 cut(s) 50, 83, 98
BstBAI YACGTR 1 cut(s) 484
BstDEI CTNAG 1 cut(s) 125
BstENI CCTNNNNNAGG 1 cut(s) 267
BstF5I GGATG 1 cut(s) 197
BstKTI GATC 3 cut(s) 480, 512, 613
BstMBI GATC 3 cut(s) 477, 509, 610
BstNI CCWGG 1 cut(s) 557
BstSCI CCNGG 2 cut(s) 516, 555
BstSLI GKGCMC 1 cut(s) 489
BsuI GTATCC 1 cut(s) 378
BsuRI GGCC 1 cut(s) 431
BtsCI GGATG 1 cut(s) 197
Cfr13I GGNCC 2 cut(s) 416, 430
Csp6I GTAC 2 cut(s) 8, 472
CviAII CATG 3 cut(s) 136, 313, 536
CviQI GTAC 2 cut(s) 8, 472
DdeI CTNAG 1 cut(s) 125
DpnI GATC 3 cut(s) 479, 511, 612
DpnII GATC 3 cut(s) 477, 509, 610
DraI TTTAAA 1 cut(s) 27
DraIII CACNNNGTG 1 cut(s) 608
Eam1104I CTCTTC 3 cut(s) 50, 83, 98
EarI CTCTTC 3 cut(s) 50, 83, 98
Eco130I CCWWGG 1 cut(s) 387
Eco47I GGWCC 1 cut(s) 416
EcoNI CCTNNNNNAGG 1 cut(s) 267
EcoO109I RGGNCCY 1 cut(s) 416
EcoRI GAATTC 1 cut(s) 546
EcoRII CCWGG 1 cut(s) 555
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 3 cut(s) 139, 316, 539
FaiI YATR 5 cut(s) 137, 269, 314, 537, 578
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FatI CATG 3 cut(s) 135, 312, 535
Fnu4HI GCNGC 2 cut(s) 100, 293
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 2 cut(s) 100, 293
FspBI CTAG 1 cut(s) 552
GluI GCNGC 2 cut(s) 100, 293
GsuI CTGGAG 1 cut(s) 510
HaeIII GGCC 1 cut(s) 431
HapII CCGG 2 cut(s) 296, 518
Hin1II CATG 3 cut(s) 139, 316, 539
HinfI GANTC 3 cut(s) 154, 349, 493
HpaII CCGG 2 cut(s) 296, 518
Hpy166II GTNNAC 1 cut(s) 487
Hpy188I TCNGA 5 cut(s) 108, 116, 357, 364, 615
Hpy188III TCNNGA 1 cut(s) 181
Hpy8I GTNNAC 1 cut(s) 487
HpyAV CCTTC 3 cut(s) 187, 271, 406
HpyCH4III ACNGT 1 cut(s) 608
HpyCH4IV ACGT 2 cut(s) 225, 483
HpyCH4V TGCA 5 cut(s) 160, 197, 487, 576, 602
HpyF3I CTNAG 1 cut(s) 125
HpySE526I ACGT 2 cut(s) 225, 483
Hsp92II CATG 3 cut(s) 139, 316, 539
Kzo9I GATC 3 cut(s) 477, 509, 610
LguI GCTCTTC 1 cut(s) 50
LmnI GCTCC 1 cut(s) 388
LpnPI CCDG 9 cut(s) 68, 309, 432, 531, 540, 542, 569, 586, 612
LweI GCATC 1 cut(s) 206
MaeI CTAG 1 cut(s) 552
MaeII ACGT 2 cut(s) 225, 483
MaeIII GTNAC 1 cut(s) 563
MalI GATC 3 cut(s) 479, 511, 612
MboI GATC 3 cut(s) 477, 509, 610
MboII GAAGA 6 cut(s) 25, 37, 70, 85, 442, 555
MhlI GDGCHC 3 cut(s) 126, 385, 489
MluCI AATT 5 cut(s) 49, 161, 170, 546, 580
MlyI GAGTC 2 cut(s) 163, 358
MmeI TCCRAC 1 cut(s) 262
MroXI GAANNNNTTC 1 cut(s) 449
MseI TTAA 1 cut(s) 26
MspI CCGG 2 cut(s) 296, 518
MspR9I CCNGG 2 cut(s) 518, 557
MvaI CCWGG 1 cut(s) 557
NciI CCSGG 1 cut(s) 518
NdeII GATC 3 cut(s) 477, 509, 610
NlaIII CATG 3 cut(s) 139, 316, 539
NlaIV GGNNCC 2 cut(s) 417, 432
PciSI GCTCTTC 1 cut(s) 50
PcsI WCGNNNNNNNCGW 2 cut(s) 262, 315
PdmI GAANNNNTTC 1 cut(s) 449
PfeI GAWTC 1 cut(s) 493
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 2 cut(s) 101, 294
PleI GAGTC 2 cut(s) 162, 357
PpsI GAGTC 2 cut(s) 162, 357
Ppu21I YACGTR 1 cut(s) 484
PpuMI RGGWCCY 1 cut(s) 416
Psp5II RGGWCCY 1 cut(s) 416
Psp6I CCWGG 1 cut(s) 555
PspGI CCWGG 1 cut(s) 555
PspN4I GGNNCC 2 cut(s) 417, 432
PspPI GGNCC 2 cut(s) 416, 430
PspPPI RGGWCCY 1 cut(s) 416
RsaI GTAC 2 cut(s) 9, 473
RsaNI GTAC 2 cut(s) 8, 472
SapI GCTCTTC 1 cut(s) 50
SaqAI TTAA 1 cut(s) 26
SatI GCNGC 2 cut(s) 100, 293
Sau3AI GATC 3 cut(s) 477, 509, 610
Sau96I GGNCC 2 cut(s) 416, 430
SchI GAGTC 2 cut(s) 163, 358
ScrFI CCNGG 2 cut(s) 518, 557
SduI GDGCHC 3 cut(s) 126, 385, 489
SfaNI GCATC 1 cut(s) 206
SinI GGWCC 1 cut(s) 416
SmlI CTYRAG 1 cut(s) 151
SmoI CTYRAG 1 cut(s) 151
Sse9I AATT 5 cut(s) 49, 161, 170, 546, 580
SsiI CCGC 4 cut(s) 99, 102, 206, 293
SspMI CTAG 1 cut(s) 552
StyD4I CCNGG 2 cut(s) 516, 555
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 608
TaiI ACGT 2 cut(s) 228, 486
TaqI TCGA 4 cut(s) 182, 344, 476, 491
TasI AATT 5 cut(s) 49, 161, 170, 546, 580
TatI WGTACW 1 cut(s) 471
TauI GCSGC 2 cut(s) 102, 295
TfiI GAWTC 1 cut(s) 493
Tru1I TTAA 1 cut(s) 26
Tru9I TTAA 1 cut(s) 26
TspDTI ATGAA 3 cut(s) 17, 152, 524
TspGWI ACGGA 1 cut(s) 360
Van91I CCANNNNNTGG 1 cut(s) 313
VneI GTGCAC 1 cut(s) 485
VpaK11BI GGWCC 1 cut(s) 416
XagI CCTNNNNNAGG 1 cut(s) 267
XapI RAATTY 2 cut(s) 546, 580
XmnI GAANNNNTTC 1 cut(s) 449
XspI CTAG 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.