MD13G1038700.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001447
Physical Location & Seq
Reverse (-)
2624195 .. 2626163
1969 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1038700.v1.1.491

Sequence Viewer

Length: 1248 bp
ATGAAGGCTCAAGATCCAAAGTCTCCAAGCTTTTTTCCAATATCATTCAATCTCCAACGCCACCTCCTCAATTACCTCTCATATTTCCTGCTCTTCGTTTTCGGATTAACCCTCGGAGTTGTACTTAGCAATCTCAGGGACCATTCATTCGGCAGCCTACAATTCGCACAGATCCCCTTCTCACCTGTGTCACCTCCTCCTCCATTCAATATTTCAGAAACCTTAAAGCCAAATGGAACCTCTCCAACAACATCCGTTGTTGGGACCAACACGTCCGTTTCGTCAAGTCCTCCTCGGATAGGGTTGAAGGACTATCTGAAGCCGCCGAAGGCGTTTCACGACATGAACAATGCAGAACTGCTGTGGAGAGCTTCGATGACTCCGCGGATTCCTGAATATCCATTCTGTCTTGTTCCAAAAGTTGCTTTCATGTTCTTGACAAGGGGGCCGGTTGTTTTGGCACCACTGTGGGAAAAGTTCTTTGCAGGGCATCACGGTTTGTACTCGATTTATGTGCACTCAAGTCCGTCCTACAATCACTCATCGTATACCGAAACTCCAGTTTTTCGTGGCCGGAGAATTCCGAGTCAGGAAGTAGAATGGGGGACGGTGAGCTTGATCGAGGCAGAGCGCCTCCTCCTGGCGAATGCTCTTCTCGACATCTCAAACCAGCGTTTTGTTCTGCTCTCAGATGCATGCATTCCCCTCTATAACTTCTCCACTGTCTACTCCTACCTCATCAACTCCACCCAAACTTTTGTCGAGGTCTATGACAATCCCAATGGAGATGGACGCGGCCGGTATGGACTCAGTGACTATCCGGGAATCACAATCGATCAATGGCGAAAAGGGTCGCAGTGGTTCCAAGCGGATCGAGACCTCGCCCTTGAATTCGTATCGGATAGAAAATACTTCCCAGTGTTCATGAAATGCAAAGGCTACTGTTTTTCGGATGAACATTATTTGCCAACGTTTGTGTCCATGAAATTTGGAGCAAAGAATGCAAATAGGACGTTGACCTGGGTTGACTGGGAAAAGGGTGGCCCACACCCTACGGAGTACGCCACCAAAAATCGGACGGCGGAGCTTTTGAGTGGCCTGAGGAATGGTTTTGGTCGGAAATGTGAATACAATGGGAGGAGTACAGATGTTTGTTTCTTATTCGCCAGGAAGTTCCCGCCAACTGCTTTGGATAACCTGCTGAAGTTTGCACCGGAGATCATGCACTTCAACACAAAGATATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

416

Amino Acids

47.19

Weight (kDa)

9.04

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 141 - 366 1.4e-72 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 271
Acc36I ACCTGC 1 cut(s) 1206
AccB1I GGYRCC 1 cut(s) 460
AccI GTMKAC 2 cut(s) 548, 726
AccII CGCG 2 cut(s) 385, 795
AciI CCGC 7 cut(s) 323, 383, 385, 795, 869, 1082, 1178
AclI AACGTT 1 cut(s) 971
AclWI GGATC 3 cut(s) 8, 166, 879
AcoI YGGCCR 2 cut(s) 571, 796
AcsI RAATTY 3 cut(s) 579, 890, 986
AcuI CTGAAG 2 cut(s) 338, 1223
AfaI GTAC 4 cut(s) 123, 503, 1061, 1144
AfiI CCNNNNNNNGG 4 cut(s) 261, 299, 640, 1074
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 5 cut(s) 49, 208, 307, 890, 1231
AjiI CACGTC 1 cut(s) 273
AjnI CCWGG 3 cut(s) 639, 1019, 1166
AleI CACNNNNGTG 1 cut(s) 466
AluBI AGCT 4 cut(s) 30, 371, 615, 1087
AluI AGCT 4 cut(s) 30, 371, 615, 1087
Alw21I GWGCWC 1 cut(s) 519
Alw26I GTCTC 2 cut(s) 27, 870
Alw44I GTGCAC 1 cut(s) 515
AlwI GGATC 3 cut(s) 8, 166, 879
AoxI GGCC 5 cut(s) 446, 571, 796, 1042, 1096
ApaLI GTGCAC 1 cut(s) 515
ApeKI GCWGC 1 cut(s) 153
ApoI RAATTY 3 cut(s) 579, 890, 986
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AspLEI GCGC 1 cut(s) 633
AspS9I GGNCC 4 cut(s) 139, 264, 446, 1043
AsuC2I CCSGG 1 cut(s) 822
AsuHPI GGTGA 3 cut(s) 174, 183, 622
AvaII GGWCC 2 cut(s) 139, 264
AxyI CCTNAGG 1 cut(s) 1100
BaeGI GKGCMC 1 cut(s) 519
BanI GGYRCC 1 cut(s) 460
Bbv12I GWGCWC 1 cut(s) 519
BbvI GCAGC 1 cut(s) 165
BccI CCATC 1 cut(s) 782
BceAI ACGGC 1 cut(s) 1095
BcgI CGANNNNNNTGC 2 cut(s) 496, 530
BciT130I CCWGG 3 cut(s) 641, 1021, 1168
BcnI CCSGG 1 cut(s) 822
BcoDI GTCTC 2 cut(s) 27, 870
BfoI RGCGCY 1 cut(s) 634
BfuAI ACCTGC 1 cut(s) 1206
BisI GCNGC 3 cut(s) 154, 323, 796
BlsI GCNGC 3 cut(s) 155, 324, 797
Bme1390I CCNGG 4 cut(s) 641, 822, 1021, 1168
Bme18I GGWCC 2 cut(s) 139, 264
BmgBI CACGTC 1 cut(s) 273
BmgT120I GGNCC 4 cut(s) 139, 264, 446, 1043
BmiI GGNNCC 6 cut(s) 140, 238, 265, 447, 462, 863
BmrFI CCNGG 4 cut(s) 641, 822, 1021, 1168
BmrI ACTGGG 2 cut(s) 911, 1039
BmsI GCATC 2 cut(s) 499, 682
BmuI ACTGGG 2 cut(s) 911, 1039
BpmI CTGGAG 1 cut(s) 543
BpuEI CTTGAG 1 cut(s) 505
BpuMI CCSGG 1 cut(s) 822
Bsa29I ATCGAT 1 cut(s) 834
BsaI GGTCTC 1 cut(s) 870
BsaJI CCNNGG 4 cut(s) 112, 293, 383, 1020
BsaWI WCCGGW 1 cut(s) 1213
BsaXI ACNNNNNCTCC 4 cut(s) 48, 78, 541, 571
Bsc4I CCNNNNNNNGG 4 cut(s) 261, 299, 640, 1074
Bse118I RCCGGY 2 cut(s) 448, 798
Bse1I ACTGG 3 cut(s) 560, 917, 1034
Bse21I CCTNAGG 1 cut(s) 1100
BseBI CCWGG 3 cut(s) 641, 1021, 1168
BseCI ATCGAT 1 cut(s) 834
BseDI CCNNGG 4 cut(s) 112, 293, 383, 1020
BseGI GGATG 2 cut(s) 251, 958
BseLI CCNNNNNNNGG 4 cut(s) 261, 299, 640, 1074
BseMII CTCAG 4 cut(s) 148, 702, 823, 1091
BseNI ACTGG 3 cut(s) 560, 917, 1034
BseRI GAGGAG 6 cut(s) 56, 186, 189, 282, 626, 1153
BseSI GKGCMC 1 cut(s) 519
BseX3I CGGCCG 1 cut(s) 796
BseXI GCAGC 1 cut(s) 165
Bsh1236I CGCG 2 cut(s) 385, 795
Bsh1285I CGRYCG 1 cut(s) 799
BshFI GGCC 5 cut(s) 448, 573, 798, 1044, 1098
BshNI GGYRCC 1 cut(s) 460
BshVI ATCGAT 1 cut(s) 834
BsiEI CGRYCG 1 cut(s) 799
BsiHKAI GWGCWC 1 cut(s) 519
BsiSI CCGG 5 cut(s) 449, 574, 799, 821, 1214
BslFI GGGAC 3 cut(s) 152, 277, 619
BslI CCNNNNNNNGG 4 cut(s) 261, 299, 640, 1074
BsmAI GTCTC 2 cut(s) 27, 870
BsmFI GGGAC 3 cut(s) 152, 277, 619
BsmI GAATGC 3 cut(s) 652, 699, 1006
BsnI GGCC 5 cut(s) 448, 573, 798, 1044, 1098
Bso31I GGTCTC 1 cut(s) 870
Bsp1286I GDGCHC 1 cut(s) 519
Bsp143I GATC 6 cut(s) 13, 171, 618, 835, 871, 1218
BspACI CCGC 7 cut(s) 323, 383, 385, 795, 869, 1082, 1178
BspANI GGCC 5 cut(s) 448, 573, 798, 1044, 1098
BspCNI CTCAG 4 cut(s) 147, 701, 822, 1092
BspDI ATCGAT 1 cut(s) 834
BspFNI CGCG 2 cut(s) 385, 795
BspHI TCATGA 1 cut(s) 924
BspLI GGNNCC 6 cut(s) 140, 238, 265, 447, 462, 863
BspMI ACCTGC 1 cut(s) 1206
BspPI GGATC 3 cut(s) 8, 166, 879
BspQI GCTCTTC 2 cut(s) 98, 657
BspT107I GGYRCC 1 cut(s) 460
BspTNI GGTCTC 1 cut(s) 870
BsrFI RCCGGY 2 cut(s) 448, 798
BsrI ACTGG 3 cut(s) 560, 917, 1034
BssAI RCCGGY 2 cut(s) 448, 798
BssECI CCNNGG 4 cut(s) 112, 293, 383, 1020
BssMI GATC 6 cut(s) 13, 171, 618, 835, 871, 1218
BssNAI GTATAC 1 cut(s) 549
Bst1107I GTATAC 1 cut(s) 549
Bst2UI CCWGG 3 cut(s) 641, 1021, 1168
Bst4CI ACNGT 5 cut(s) 468, 497, 610, 724, 944
Bst6I CTCTTC 2 cut(s) 98, 657
BstAPI GCANNNNNTGC 1 cut(s) 1001
BstC8I GCNNGC 1 cut(s) 697
BstDEI CTNAG 5 cut(s) 125, 134, 688, 809, 1100
BstDSI CCRYGG 1 cut(s) 383
BstENI CCTNNNNNAGG 1 cut(s) 297
BstF5I GGATG 2 cut(s) 251, 958
BstFNI CGCG 2 cut(s) 385, 795
BstH2I RGCGCY 1 cut(s) 634
BstHHI GCGC 1 cut(s) 633
BstKTI GATC 6 cut(s) 16, 174, 621, 838, 874, 1221
BstMAI GTCTC 2 cut(s) 27, 870
BstMBI GATC 6 cut(s) 13, 171, 618, 835, 871, 1218
BstMCI CGRYCG 1 cut(s) 799
BstMWI GCNNNNNNNGC 1 cut(s) 1001
BstNI CCWGG 3 cut(s) 641, 1021, 1168
BstNSI RCATGY 1 cut(s) 699
BstSCI CCNGG 4 cut(s) 639, 820, 1019, 1166
BstSLI GKGCMC 1 cut(s) 519
BstUI CGCG 2 cut(s) 385, 795
BstV1I GCAGC 1 cut(s) 165
BstX2I RGATCY 2 cut(s) 13, 171
BstYI RGATCY 2 cut(s) 13, 171
BstZ17I GTATAC 1 cut(s) 549
BstZI CGGCCG 1 cut(s) 796
Bsu15I ATCGAT 1 cut(s) 834
Bsu36I CCTNAGG 1 cut(s) 1100
BsuRI GGCC 5 cut(s) 448, 573, 798, 1044, 1098
BsuTUI ATCGAT 1 cut(s) 834
BtgI CCRYGG 1 cut(s) 383
BtrI CACGTC 1 cut(s) 273
BtsCI GGATG 2 cut(s) 251, 958
BtsI GCAGTG 1 cut(s) 863
BtsIMutI CAGTG 5 cut(s) 464, 720, 817, 863, 924
BveI ACCTGC 1 cut(s) 1206
Cac8I GCNNGC 1 cut(s) 697
CciI TCATGA 1 cut(s) 924
CfoI GCGC 1 cut(s) 633
Cfr10I RCCGGY 2 cut(s) 448, 798
Cfr13I GGNCC 4 cut(s) 139, 264, 446, 1043
Cfr42I CCGCGG 1 cut(s) 386
ClaI ATCGAT 1 cut(s) 834
CseI GACGC 1 cut(s) 801
Csp6I GTAC 4 cut(s) 122, 502, 1060, 1143
CviAII CATG 6 cut(s) 343, 430, 696, 925, 982, 1222
CviQI GTAC 4 cut(s) 122, 502, 1060, 1143
DdeI CTNAG 5 cut(s) 125, 134, 688, 809, 1100
DpnI GATC 6 cut(s) 15, 173, 620, 837, 873, 1220
DpnII GATC 6 cut(s) 13, 171, 618, 835, 871, 1218
DrdI GACNNNNNNGTC 1 cut(s) 271
DseDI GACNNNNNNGTC 1 cut(s) 271
EaeI YGGCCR 2 cut(s) 571, 796
EagI CGGCCG 1 cut(s) 796
Eam1104I CTCTTC 2 cut(s) 98, 657
EarI CTCTTC 2 cut(s) 98, 657
EciI GGCGGA 1 cut(s) 1097
EclXI CGGCCG 1 cut(s) 796
Eco31I GGTCTC 1 cut(s) 870
Eco47I GGWCC 2 cut(s) 139, 264
Eco52I CGGCCG 1 cut(s) 796
Eco57I CTGAAG 2 cut(s) 338, 1223
Eco81I CCTNAGG 1 cut(s) 1100
EcoNI CCTNNNNNAGG 1 cut(s) 297
EcoRI GAATTC 2 cut(s) 579, 890
EcoRII CCWGG 3 cut(s) 639, 1019, 1166
EcoT22I ATGCAT 2 cut(s) 697, 701
FaeI CATG 6 cut(s) 346, 433, 699, 928, 985, 1225
FaqI GGGAC 3 cut(s) 152, 277, 619
FatI CATG 6 cut(s) 342, 429, 695, 924, 981, 1221
FauI CCCGC 1 cut(s) 1185
FblI GTMKAC 2 cut(s) 548, 726
Fnu4HI GCNGC 3 cut(s) 154, 323, 796
FokI GGATG 2 cut(s) 238, 965
Fsp4HI GCNGC 3 cut(s) 154, 323, 796
GlaI GCGC 1 cut(s) 632
GluI GCNGC 3 cut(s) 154, 323, 796
GsuI CTGGAG 1 cut(s) 543
HaeII RGCGCY 1 cut(s) 634
HaeIII GGCC 5 cut(s) 448, 573, 798, 1044, 1098
HapII CCGG 5 cut(s) 449, 574, 799, 821, 1214
HgaI GACGC 1 cut(s) 801
HhaI GCGC 1 cut(s) 633
Hin1II CATG 6 cut(s) 346, 433, 699, 928, 985, 1225
Hin6I GCGC 1 cut(s) 631
HinP1I GCGC 1 cut(s) 631
HincII GTYRAC 2 cut(s) 1017, 1027
HindII GTYRAC 2 cut(s) 1017, 1027
HindIII AAGCTT 1 cut(s) 28
HinfI GANTC 5 cut(s) 379, 388, 586, 807, 825
HpaII CCGG 5 cut(s) 449, 574, 799, 821, 1214
HphI GGTGA 3 cut(s) 174, 183, 622
Hpy166II GTNNAC 5 cut(s) 517, 549, 727, 1017, 1027
Hpy188III TCNNGA 8 cut(s) 11, 338, 392, 436, 590, 656, 875, 925
Hpy8I GTNNAC 5 cut(s) 517, 549, 727, 1017, 1027
HpyAV CCTTC 3 cut(s) 187, 301, 322
HpyCH4III ACNGT 5 cut(s) 468, 497, 610, 724, 944
HpyCH4IV ACGT 3 cut(s) 272, 971, 1013
HpyCH4V TGCA 9 cut(s) 353, 485, 517, 695, 699, 933, 1004, 1211, 1225
HpyF10VI GCNNNNNNNGC 1 cut(s) 1001
HpyF3I CTNAG 5 cut(s) 125, 134, 688, 809, 1100
HpySE526I ACGT 3 cut(s) 272, 971, 1013
Hsp92II CATG 6 cut(s) 346, 433, 699, 928, 985, 1225
HspAI GCGC 1 cut(s) 631
KspI CCGCGG 1 cut(s) 386
Kzo9I GATC 6 cut(s) 13, 171, 618, 835, 871, 1218
LguI GCTCTTC 2 cut(s) 98, 657
LmnI GCTCC 2 cut(s) 992, 1084
Lsp1109I GCAGC 1 cut(s) 165
LweI GCATC 2 cut(s) 499, 682
MaeII ACGT 3 cut(s) 272, 971, 1013
MaeIII GTNAC 2 cut(s) 189, 812
MalI GATC 6 cut(s) 15, 173, 620, 837, 873, 1220
MboI GATC 6 cut(s) 13, 171, 618, 835, 871, 1218
MboII GAAGA 2 cut(s) 85, 644
MflI RGATCY 2 cut(s) 13, 171
MhlI GDGCHC 1 cut(s) 519
MluCI AATT 5 cut(s) 70, 161, 579, 890, 986
MlyI GAGTC 3 cut(s) 373, 595, 801
MmeI TCCRAC 3 cut(s) 79, 269, 1097
Mph1103I ATGCAT 2 cut(s) 697, 701
MseI TTAA 2 cut(s) 107, 224
MslI CAYNNNNRTG 1 cut(s) 466
MspA1I CMGCKG 1 cut(s) 385
MspI CCGG 5 cut(s) 449, 574, 799, 821, 1214
MspR9I CCNGG 4 cut(s) 641, 822, 1021, 1168
Mva1269I GAATGC 3 cut(s) 652, 699, 1006
MvaI CCWGG 3 cut(s) 641, 1021, 1168
MvnI CGCG 2 cut(s) 385, 795
MwoI GCNNNNNNNGC 1 cut(s) 1001
NciI CCSGG 1 cut(s) 822
NdeII GATC 6 cut(s) 13, 171, 618, 835, 871, 1218
NlaIII CATG 6 cut(s) 346, 433, 699, 928, 985, 1225
NlaIV GGNNCC 6 cut(s) 140, 238, 265, 447, 462, 863
NmuCI GTSAC 2 cut(s) 189, 812
NsiI ATGCAT 2 cut(s) 697, 701
NspI RCATGY 1 cut(s) 699
OliI CACNNNNGTG 1 cut(s) 466
PaeI GCATGC 1 cut(s) 699
PagI TCATGA 1 cut(s) 924
PciSI GCTCTTC 2 cut(s) 98, 657
PcsI WCGNNNNNNNCGW 1 cut(s) 278
PctI GAATGC 3 cut(s) 652, 699, 1006
PfeI GAWTC 2 cut(s) 388, 825
PfoI TCCNGGA 1 cut(s) 820
PkrI GCNGC 3 cut(s) 155, 324, 797
PleI GAGTC 3 cut(s) 373, 594, 801
PpsI GAGTC 3 cut(s) 373, 594, 801
Psp1406I AACGTT 1 cut(s) 971
Psp6I CCWGG 3 cut(s) 639, 1019, 1166
PspGI CCWGG 3 cut(s) 639, 1019, 1166
PspN4I GGNNCC 6 cut(s) 140, 238, 265, 447, 462, 863
PspPI GGNCC 4 cut(s) 139, 264, 446, 1043
PsuI RGATCY 2 cut(s) 13, 171
RsaI GTAC 4 cut(s) 123, 503, 1061, 1144
RsaNI GTAC 4 cut(s) 122, 502, 1060, 1143
RseI CAYNNNNRTG 1 cut(s) 466
SacII CCGCGG 1 cut(s) 386
SapI GCTCTTC 2 cut(s) 98, 657
SaqAI TTAA 2 cut(s) 107, 224
SatI GCNGC 3 cut(s) 154, 323, 796
Sau3AI GATC 6 cut(s) 13, 171, 618, 835, 871, 1218
Sau96I GGNCC 4 cut(s) 139, 264, 446, 1043
SchI GAGTC 3 cut(s) 373, 595, 801
ScrFI CCNGG 4 cut(s) 641, 822, 1021, 1168
SduI GDGCHC 1 cut(s) 519
SfaNI GCATC 2 cut(s) 499, 682
Sfr303I CCGCGG 1 cut(s) 386
SgrBI CCGCGG 1 cut(s) 386
SinI GGWCC 2 cut(s) 139, 264
SmiMI CAYNNNNRTG 1 cut(s) 466
SmlI CTYRAG 2 cut(s) 9, 520
SmoI CTYRAG 2 cut(s) 9, 520
SphI GCATGC 1 cut(s) 699
Sse9I AATT 5 cut(s) 70, 161, 579, 890, 986
SsiI CCGC 7 cut(s) 323, 383, 385, 795, 869, 1082, 1178
SspI AATATT 1 cut(s) 211
StyD4I CCNGG 4 cut(s) 639, 820, 1019, 1166
TaaI ACNGT 5 cut(s) 468, 497, 610, 724, 944
TaiI ACGT 3 cut(s) 275, 974, 1016
TaqI TCGA 7 cut(s) 374, 506, 621, 657, 762, 834, 874
TasI AATT 5 cut(s) 70, 161, 579, 890, 986
TatI WGTACW 3 cut(s) 121, 501, 1142
TauI GCSGC 2 cut(s) 325, 798
TfiI GAWTC 2 cut(s) 388, 825
Tru1I TTAA 2 cut(s) 107, 224
Tru9I TTAA 2 cut(s) 107, 224
TscAI CASTG 5 cut(s) 471, 727, 817, 863, 924
TseFI GTSAC 2 cut(s) 189, 812
TseI GCWGC 1 cut(s) 153
Tsp45I GTSAC 2 cut(s) 189, 812
TspDTI ATGAA 8 cut(s) 17, 135, 359, 418, 913, 941, 969, 998
TspGWI ACGGA 4 cut(s) 244, 265, 516, 1070
TspRI CASTG 5 cut(s) 471, 727, 817, 863, 924
VneI GTGCAC 1 cut(s) 515
VpaK11BI GGWCC 2 cut(s) 139, 264
XagI CCTNNNNNAGG 1 cut(s) 297
XapI RAATTY 3 cut(s) 579, 890, 986
XceI RCATGY 1 cut(s) 699
XmiI GTMKAC 2 cut(s) 548, 726
Zsp2I ATGCAT 2 cut(s) 697, 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.