Rh4BG343000

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001447
Physical Location & Seq
Reverse (-)
50584718 .. 50586157
1440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG343000.1

Sequence Viewer

Length: 882 bp
ATGAAGGATCATAATCAAAACCCTTCAAACTTCTTCACAAAACTAATCAATGTCCAAGGCCACCTCCTCAATCTCCTCTCTTATTTCCTCATCTTTGGTGGTGGTTTAGTTCTCGGAATCGTACTTGCCTCTTCTTATCTCAAAGACTCTTCCTTTAGTCTACAAATAGCCCAACTATCATTCTCCTCTTCTTCTTCTTCATCATCTCCGTCCAATAGTTCCAATATTTCACATATGACAATTAGTCCAAATGACACAGTCACGATGTCTAACACGACCGAAGTTCATATAGGAGAATTAGAGGCATTTCTAAAGCCGCCTAAGGTTCGGCACGACATGGACGATGATGAATTGTTCTGGAGAGCTTCCATGGCCCCTCGGATACCCGAATATCCGTTCCACCGAGTTCCGAAAGTTGCTTTCATGTTCTTGACAAGGGGACCTGTTCATTTGGCTCCATTGTGGGAGAAGTTCTTCCAAGGGCACCAAGGCTTGTTCTCAATCTATGTCCACTCAGATCCGTCTTATAATGAATCGTCTCACCCCGAAAGCTCAGTCTTCCATGGCCGAAGAATTCCAAGTCAGAGAGCAGAATGGGGGCAAGTGAACATGATTGAAGCCGAGAGCCGCCTAATTGCGAATGCTCTTCTCGACATCTCAAACCAACGTTTTGTTCTGCTTTCAGAATCATGCATCCCCCTCTTCAATTTCTCCACAGTCTACTCTTACCTCATAAACACCAACAAGACTTTTGTGCAGGTCTATGATGATCCAAGCGGAGTTGGGCGTGGCCGTTATGATGAAAGCTACTACCCTCAAATCACATTGGACCAGTGGAGGAAAGGGTCGCAGTGGGTTGAAATAGATAGACCTTGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

293

Amino Acids

33.31

Weight (kDa)

6.16

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 139 - 291 1.4e-51 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 528
Acc36I ACCTGC 1 cut(s) 748
AccB1I GGYRCC 1 cut(s) 483
AccI GTMKAC 2 cut(s) 160, 720
AciI CCGC 3 cut(s) 317, 628, 777
AclI AACGTT 1 cut(s) 667
AclWI GGATC 3 cut(s) 15, 512, 764
AcoI YGGCCR 2 cut(s) 565, 790
AcsI RAATTY 1 cut(s) 573
AfaI GTAC 1 cut(s) 123
AgsI TTSAA 4 cut(s) 27, 617, 706, 860
AhdI GACNNNNNGTC 1 cut(s) 243
AjuI GAANNNNNNNTTGG 2 cut(s) 657, 689
AluBI AGCT 3 cut(s) 365, 552, 807
AluI AGCT 3 cut(s) 365, 552, 807
Alw26I GTCTC 1 cut(s) 543
AlwI GGATC 3 cut(s) 15, 512, 764
AoxI GGCC 4 cut(s) 58, 372, 565, 790
ApoI RAATTY 1 cut(s) 573
ArsI GACNNNNNNTTYG 2 cut(s) 540, 572
Asp700I GAANNNNTTC 1 cut(s) 473
AspS9I GGNCC 3 cut(s) 373, 440, 829
AsuHPI GGTGA 1 cut(s) 533
AvaII GGWCC 2 cut(s) 440, 829
AxyI CCTNAGG 1 cut(s) 321
BaeGI GKGCMC 1 cut(s) 486
BanI GGYRCC 1 cut(s) 483
BbsI GAAGAC 1 cut(s) 550
BceAI ACGGC 1 cut(s) 777
BciVI GTATCC 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 543
BfuAI ACCTGC 1 cut(s) 748
BfuI GTATCC 1 cut(s) 375
BisI GCNGC 2 cut(s) 317, 628
BlsI GCNGC 2 cut(s) 318, 629
Bme18I GGWCC 2 cut(s) 440, 829
BmeRI GACNNNNNGTC 1 cut(s) 243
BmgT120I GGNCC 3 cut(s) 373, 440, 829
BmiI GGNNCC 4 cut(s) 375, 441, 456, 485
BmsI GCATC 1 cut(s) 702
BpiI GAAGAC 1 cut(s) 550
BpmI CTGGAG 1 cut(s) 379
BsaBI GATNNNNATC 1 cut(s) 12
BsaJI CCNNGG 6 cut(s) 55, 369, 377, 478, 487, 562
Bse1I ACTGG 1 cut(s) 832
Bse21I CCTNAGG 1 cut(s) 321
Bse8I GATNNNNATC 1 cut(s) 12
BseDI CCNNGG 6 cut(s) 55, 369, 377, 478, 487, 562
BseGI GGATG 1 cut(s) 693
BseJI GATNNNNATC 1 cut(s) 12
BseMII CTCAG 2 cut(s) 528, 567
BseNI ACTGG 1 cut(s) 832
BseRI GAGGAG 3 cut(s) 56, 65, 175
BseSI GKGCMC 1 cut(s) 486
BsgI GTGCAG 1 cut(s) 776
Bsh1285I CGRYCG 1 cut(s) 279
BshFI GGCC 4 cut(s) 60, 374, 567, 792
BshNI GGYRCC 1 cut(s) 483
BsiEI CGRYCG 1 cut(s) 279
BslFI GGGAC 1 cut(s) 453
BsmAI GTCTC 1 cut(s) 543
BsmBI CGTCTC 1 cut(s) 543
BsmFI GGGAC 1 cut(s) 453
BsmI GAATGC 1 cut(s) 646
BsnI GGCC 4 cut(s) 60, 374, 567, 792
Bsp1286I GDGCHC 1 cut(s) 486
Bsp143I GATC 3 cut(s) 7, 517, 769
Bsp19I CCATGG 2 cut(s) 369, 562
BspACI CCGC 3 cut(s) 317, 628, 777
BspANI GGCC 4 cut(s) 60, 374, 567, 792
BspCNI CTCAG 2 cut(s) 527, 566
BspLI GGNNCC 4 cut(s) 375, 441, 456, 485
BspMI ACCTGC 1 cut(s) 748
BspPI GGATC 3 cut(s) 15, 512, 764
BspQI GCTCTTC 1 cut(s) 651
BspT107I GGYRCC 1 cut(s) 483
BsrI ACTGG 1 cut(s) 832
BssECI CCNNGG 6 cut(s) 55, 369, 377, 478, 487, 562
BssMI GATC 3 cut(s) 7, 517, 769
BssT1I CCWWGG 5 cut(s) 55, 369, 478, 487, 562
Bst4CI ACNGT 2 cut(s) 259, 718
Bst6I CTCTTC 5 cut(s) 136, 154, 193, 651, 707
BstDEI CTNAG 3 cut(s) 321, 514, 553
BstDSI CCRYGG 2 cut(s) 369, 562
BstF5I GGATG 1 cut(s) 693
BstKTI GATC 3 cut(s) 10, 520, 772
BstMAI GTCTC 1 cut(s) 543
BstMBI GATC 3 cut(s) 7, 517, 769
BstMCI CGRYCG 1 cut(s) 279
BstMWI GCNNNNNNNGC 1 cut(s) 371
BstSLI GKGCMC 1 cut(s) 486
BstV2I GAAGAC 1 cut(s) 550
BstX2I RGATCY 1 cut(s) 517
BstYI RGATCY 1 cut(s) 517
Bsu36I CCTNAGG 1 cut(s) 321
BsuI GTATCC 1 cut(s) 375
BsuRI GGCC 4 cut(s) 60, 374, 567, 792
BtgI CCRYGG 2 cut(s) 369, 562
BtsCI GGATG 1 cut(s) 693
BtsI GCAGTG 1 cut(s) 857
BtsIMutI CAGTG 2 cut(s) 839, 857
BveI ACCTGC 1 cut(s) 748
Cfr13I GGNCC 3 cut(s) 373, 440, 829
Csp6I GTAC 1 cut(s) 122
CviAII CATG 6 cut(s) 337, 370, 424, 563, 610, 690
CviQI GTAC 1 cut(s) 122
DdeI CTNAG 3 cut(s) 321, 514, 553
DpnI GATC 3 cut(s) 9, 519, 771
DpnII GATC 3 cut(s) 7, 517, 769
DriI GACNNNNNGTC 1 cut(s) 243
EaeI YGGCCR 2 cut(s) 565, 790
Eam1104I CTCTTC 5 cut(s) 136, 154, 193, 651, 707
Eam1105I GACNNNNNGTC 1 cut(s) 243
EarI CTCTTC 5 cut(s) 136, 154, 193, 651, 707
Eco130I CCWWGG 5 cut(s) 55, 369, 478, 487, 562
Eco47I GGWCC 2 cut(s) 440, 829
Eco81I CCTNAGG 1 cut(s) 321
EcoO109I RGGNCCY 1 cut(s) 440
EcoRI GAATTC 1 cut(s) 573
EcoT14I CCWWGG 5 cut(s) 55, 369, 478, 487, 562
EcoT22I ATGCAT 1 cut(s) 695
ErhI CCWWGG 5 cut(s) 55, 369, 478, 487, 562
Esp3I CGTCTC 1 cut(s) 543
FaeI CATG 6 cut(s) 340, 373, 427, 566, 613, 693
FaqI GGGAC 1 cut(s) 453
FatI CATG 6 cut(s) 336, 369, 423, 562, 609, 689
FauNDI CATATG 1 cut(s) 234
FblI GTMKAC 2 cut(s) 160, 720
Fnu4HI GCNGC 2 cut(s) 317, 628
FokI GGATG 1 cut(s) 680
Fsp4HI GCNGC 2 cut(s) 317, 628
GluI GCNGC 2 cut(s) 317, 628
GsuI CTGGAG 1 cut(s) 379
HaeIII GGCC 4 cut(s) 60, 374, 567, 792
Hin1II CATG 6 cut(s) 340, 373, 427, 566, 613, 693
HinfI GANTC 4 cut(s) 117, 146, 533, 686
HphI GGTGA 1 cut(s) 533
Hpy166II GTNNAC 4 cut(s) 161, 511, 607, 721
Hpy188I TCNGA 6 cut(s) 116, 381, 411, 517, 585, 685
Hpy188III TCNNGA 4 cut(s) 262, 358, 430, 650
Hpy8I GTNNAC 4 cut(s) 161, 511, 607, 721
HpyAV CCTTC 1 cut(s) 33
HpyCH4III ACNGT 2 cut(s) 259, 718
HpyCH4IV ACGT 1 cut(s) 667
HpyCH4V TGCA 2 cut(s) 693, 757
HpyF10VI GCNNNNNNNGC 1 cut(s) 371
HpyF3I CTNAG 3 cut(s) 321, 514, 553
HpySE526I ACGT 1 cut(s) 667
Hsp92II CATG 6 cut(s) 340, 373, 427, 566, 613, 693
Kzo9I GATC 3 cut(s) 7, 517, 769
LguI GCTCTTC 1 cut(s) 651
LmnI GCTCC 1 cut(s) 460
LpnPI CCDG 4 cut(s) 343, 456, 743, 845
LweI GCATC 1 cut(s) 702
MaeII ACGT 1 cut(s) 667
MaeIII GTNAC 1 cut(s) 259
MalI GATC 3 cut(s) 9, 519, 771
MboI GATC 3 cut(s) 7, 517, 769
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 1 cut(s) 486
MluCI AATT 6 cut(s) 240, 296, 350, 573, 633, 706
MlyI GAGTC 1 cut(s) 140
Mph1103I ATGCAT 1 cut(s) 695
MroXI GAANNNNTTC 1 cut(s) 473
Mva1269I GAATGC 1 cut(s) 646
MwoI GCNNNNNNNGC 1 cut(s) 371
NcoI CCATGG 2 cut(s) 369, 562
NdeI CATATG 1 cut(s) 234
NdeII GATC 3 cut(s) 7, 517, 769
NlaIII CATG 6 cut(s) 340, 373, 427, 566, 613, 693
NlaIV GGNNCC 4 cut(s) 375, 441, 456, 485
NmeAIII GCCGAG 1 cut(s) 646
NmuCI GTSAC 1 cut(s) 259
NsiI ATGCAT 1 cut(s) 695
PciSI GCTCTTC 1 cut(s) 651
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 646
PdmI GAANNNNTTC 1 cut(s) 473
PfeI GAWTC 3 cut(s) 117, 533, 686
PflFI GACNNNGTC 1 cut(s) 257
PkrI GCNGC 2 cut(s) 318, 629
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PpuMI RGGWCCY 1 cut(s) 440
PsiI TTATAA 1 cut(s) 528
Psp1406I AACGTT 1 cut(s) 667
Psp5II RGGWCCY 1 cut(s) 440
PspN4I GGNNCC 4 cut(s) 375, 441, 456, 485
PspPI GGNCC 3 cut(s) 373, 440, 829
PspPPI RGGWCCY 1 cut(s) 440
PsuI RGATCY 1 cut(s) 517
PsyI GACNNNGTC 1 cut(s) 257
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
SapI GCTCTTC 1 cut(s) 651
SatI GCNGC 2 cut(s) 317, 628
Sau3AI GATC 3 cut(s) 7, 517, 769
Sau96I GGNCC 3 cut(s) 373, 440, 829
SchI GAGTC 1 cut(s) 140
SduI GDGCHC 1 cut(s) 486
SfaNI GCATC 1 cut(s) 702
SinI GGWCC 2 cut(s) 440, 829
Sse9I AATT 6 cut(s) 240, 296, 350, 573, 633, 706
SsiI CCGC 3 cut(s) 317, 628, 777
SspI AATATT 1 cut(s) 226
StyI CCWWGG 5 cut(s) 55, 369, 478, 487, 562
TaaI ACNGT 2 cut(s) 259, 718
TaiI ACGT 1 cut(s) 670
TaqI TCGA 1 cut(s) 651
TaqII GACCGA 1 cut(s) 293
TasI AATT 6 cut(s) 240, 296, 350, 573, 633, 706
TauI GCSGC 2 cut(s) 319, 630
TfiI GAWTC 3 cut(s) 117, 533, 686
TscAI CASTG 2 cut(s) 839, 857
TseFI GTSAC 1 cut(s) 259
Tsp45I GTSAC 1 cut(s) 259
TspDTI ATGAA 8 cut(s) 17, 189, 275, 363, 412, 437, 546, 816
TspGWI ACGGA 3 cut(s) 198, 384, 510
TspRI CASTG 2 cut(s) 839, 857
Tth111I GACNNNGTC 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 440, 829
XapI RAATTY 1 cut(s) 573
XmiI GTMKAC 2 cut(s) 160, 720
XmnI GAANNNNTTC 1 cut(s) 473
Zsp2I ATGCAT 1 cut(s) 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.