RchiOBHm_Chr4g0434751

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001447
Physical Location & Seq
Reverse (-)
58421751 .. 58423978
2228 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40320

Sequence Viewer

Length: 1224 bp
ATGAAGGGTGGTAGTGGTGGTTCTACTCAGAATCTGAGCACTGTAAAATTTTTTCCAAAGCTCATCAATGTCCAACTCCATCACCTCATCAGTGTCCTCTCTTATTTCCTCTTATTTGGTGTTGGAATAACCCTCGGGATGATCCTTACCTTCTATCTCAAAGACTTTTCTTTCAGTCTCCAACTGGCCCAATTCTCCTTCTCAACCTCATCATCTTCATCTAATCGAAACACGATACTGCCAAGTGTGACAGCTGCGCAAACCAATTTGAGTCACCCCCAGCACATCGGATTGGAGAAATTTCTGAAACCCCCTAAGATTTTGCACGACATGAATGATGAAGAATTGCTATGGAGAGCTTCTATGGCTCCACGGAGATCCGGATATCCATTCCATCGAGTTCCGAAAGTTGCCTTCTTGTTCTTGACAAAGGGACCAGTTTTTCTGGCCCCGTTGTGGGAGAAGTTCTTCGAAGGGCATCAAGGTTTGTACTCGATATATGTGCACTCGAATCCGTCTTACAATGGATCGTACCCCGAAAGTCCGGTGTTTCAAGGCAGAAGAATTCCTAGTCAGGAAGTGGAATGGGGAAATGTGAATATGATTGAGGCTGAGCGCCGCCTATTGGCAAATGCCCTTCTTGATATCTCAAACGATCGCTTTGTTCTTCTCTCGGAGTCATGCATCCCCCTCCACAACTTCAGCACCATCTACCACTTCCTTGTAAATTCAAAGCAGACATTTGTTCAGGTCTATGACGATCCAAGTAGTGTTGGGCGCGGGAGATACAGTTCACACATGTACCCGCAAATCTCATTGTCACAATGGAGGAAAGGGTCTCAGTGGTTCCAGATAGATAGAGACATTGCCACTGAAGTTGTCGCAGACAAAAAGTACTTCCCACTTTTCCAGAAGTACTGCAGGGGCTCATGCTACGCAGACGAGCATTACTTCCCAACATTTGTTCATATGAAATTTTCTGCAAAGAATTCAAATAAAACCTTGACTTGGTATGACTTTTCGAGGGGTGGACCGCACCCTGCCCAGTTCACCAGAACAGACGTGACGGTTAATCTGCTGAACAGCTTCAGGAATGGTACCAAATGTTTTGATTATCTAATGACAGGCAAAGTTTGTCCTCTCTTGTTTGCTAGGAAGTTCACTCCTGGCACTTTGGAGAGACTGCTGCGGTTTGCACCAAAATTGATGCAGTTCAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

407

Amino Acids

46.71

Weight (kDa)

9.59

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 137 - 362 2.4e-79 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 258
Acc65I GGTACC 1 cut(s) 1097
AccB1I GGYRCC 1 cut(s) 1097
AccII CGCG 1 cut(s) 780
AccIII TCCGGA 1 cut(s) 380
AciI CCGC 5 cut(s) 619, 780, 806, 1034, 1189
AclWI GGATC 4 cut(s) 136, 372, 535, 755
AcsI RAATTY 6 cut(s) 47, 299, 564, 727, 974, 988
AcuI CTGAAG 3 cut(s) 685, 894, 1072
AfaI GTAC 6 cut(s) 491, 533, 803, 896, 917, 1099
AfiI CCNNNNNNNGG 4 cut(s) 456, 457, 625, 1008
AflIII ACRYGT 1 cut(s) 798
AgsI TTSAA 4 cut(s) 554, 732, 993, 1216
AjiI CACGTC 1 cut(s) 1063
AjnI CCWGG 1 cut(s) 1165
AloI GAACNNNNNNTCC 2 cut(s) 775, 807
AluBI AGCT 4 cut(s) 61, 254, 359, 1086
AluI AGCT 4 cut(s) 61, 254, 359, 1086
Alw21I GWGCWC 2 cut(s) 41, 507
Alw26I GTCTC 4 cut(s) 182, 843, 855, 1174
Alw44I GTGCAC 1 cut(s) 503
AlwI GGATC 4 cut(s) 136, 372, 535, 755
AlwNI CAGNNNCTG 1 cut(s) 34
Ama87I CYCGRG 1 cut(s) 134
Aor13HI TCCGGA 1 cut(s) 380
AoxI GGCC 2 cut(s) 186, 447
ApaLI GTGCAC 1 cut(s) 503
ApeKI GCWGC 2 cut(s) 254, 1186
ApoI RAATTY 6 cut(s) 47, 299, 564, 727, 974, 988
Asp700I GAANNNNTTC 2 cut(s) 467, 1085
Asp718I GGTACC 1 cut(s) 1097
AspLEI GCGC 3 cut(s) 259, 618, 780
AspS9I GGNCC 4 cut(s) 187, 434, 448, 1031
AsuHPI GGTGA 3 cut(s) 74, 266, 1042
AsuII TTCGAA 1 cut(s) 471
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 2 cut(s) 434, 1031
BaeGI GKGCMC 1 cut(s) 507
BaeI ACNNNNGTAYC 2 cut(s) 785, 818
BanI GGYRCC 1 cut(s) 1097
BanII GRGCYC 1 cut(s) 929
Bbv12I GWGCWC 2 cut(s) 41, 507
BbvI GCAGC 2 cut(s) 241, 1173
BccI CCATC 3 cut(s) 87, 402, 716
BcgI CGANNNNNNTGC 2 cut(s) 484, 518
BciT130I CCWGG 1 cut(s) 1167
BcoDI GTCTC 4 cut(s) 182, 843, 855, 1174
BfaI CTAG 2 cut(s) 570, 1152
BfmI CTRYAG 1 cut(s) 919
BfoI RGCGCY 1 cut(s) 619
BisI GCNGC 3 cut(s) 255, 619, 1187
BlpI GCTNAGC 1 cut(s) 612
BlsI GCNGC 3 cut(s) 256, 620, 1188
BmcAI AGTACT 2 cut(s) 896, 917
Bme1390I CCNGG 1 cut(s) 1167
Bme18I GGWCC 2 cut(s) 434, 1031
BmeT110I CYCGRG 1 cut(s) 134
BmgBI CACGTC 1 cut(s) 1063
BmgT120I GGNCC 4 cut(s) 187, 434, 448, 1031
BmiI GGNNCC 5 cut(s) 369, 435, 450, 848, 1099
BmrFI CCNGG 1 cut(s) 1167
BmrI ACTGGG 1 cut(s) 1039
BmsI GCATC 3 cut(s) 487, 693, 1197
BmuI ACTGGG 1 cut(s) 1039
Bpu1102I GCTNAGC 1 cut(s) 612
Bpu14I TTCGAA 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 843
BsaJI CCNNGG 2 cut(s) 133, 371
BsaWI WCCGGW 2 cut(s) 380, 544
BsaXI ACNNNNNCTCC 2 cut(s) 775, 805
Bsc4I CCNNNNNNNGG 4 cut(s) 456, 457, 625, 1008
Bse1I ACTGG 3 cut(s) 189, 437, 1045
Bse3DI GCAATG 1 cut(s) 864
BseAI TCCGGA 1 cut(s) 380
BseBI CCWGG 1 cut(s) 1167
BseDI CCNNGG 2 cut(s) 133, 371
BseGI GGATG 2 cut(s) 144, 684
BseLI CCNNNNNNNGG 4 cut(s) 456, 457, 625, 1008
BseMI GCAATG 1 cut(s) 864
BseMII CTCAG 4 cut(s) 26, 41, 603, 854
BseNI ACTGG 3 cut(s) 189, 437, 1045
BseSI GKGCMC 1 cut(s) 507
BseXI GCAGC 2 cut(s) 241, 1173
BseYI CCCAGC 1 cut(s) 279
Bsh1236I CGCG 1 cut(s) 780
Bsh1285I CGRYCG 1 cut(s) 658
BshFI GGCC 2 cut(s) 188, 449
BshNI GGYRCC 1 cut(s) 1097
BsiEI CGRYCG 1 cut(s) 658
BsiHKAI GWGCWC 2 cut(s) 41, 507
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 2 cut(s) 381, 545
BslFI GGGAC 1 cut(s) 447
BslI CCNNNNNNNGG 4 cut(s) 456, 457, 625, 1008
BsmAI GTCTC 4 cut(s) 182, 843, 855, 1174
BsmFI GGGAC 1 cut(s) 447
BsnI GGCC 2 cut(s) 188, 449
Bso31I GGTCTC 1 cut(s) 843
BsoBI CYCGRG 1 cut(s) 134
Bsp119I TTCGAA 1 cut(s) 471
Bsp1286I GDGCHC 3 cut(s) 41, 507, 929
Bsp13I TCCGGA 1 cut(s) 380
Bsp143I GATC 5 cut(s) 141, 377, 527, 655, 760
Bsp1720I GCTNAGC 1 cut(s) 612
BspACI CCGC 5 cut(s) 619, 780, 806, 1034, 1189
BspANI GGCC 2 cut(s) 188, 449
BspCNI CTCAG 4 cut(s) 27, 40, 604, 853
BspEI TCCGGA 1 cut(s) 380
BspFNI CGCG 1 cut(s) 780
BspLI GGNNCC 5 cut(s) 369, 435, 450, 848, 1099
BspMAI CTGCAG 1 cut(s) 923
BspPI GGATC 4 cut(s) 136, 372, 535, 755
BspT104I TTCGAA 1 cut(s) 471
BspT107I GGYRCC 1 cut(s) 1097
BspTNI GGTCTC 1 cut(s) 843
BsrDI GCAATG 1 cut(s) 864
BsrI ACTGG 3 cut(s) 189, 437, 1045
BssECI CCNNGG 2 cut(s) 133, 371
BssMI GATC 5 cut(s) 141, 377, 527, 655, 760
Bst2UI CCWGG 1 cut(s) 1167
Bst4CI ACNGT 3 cut(s) 43, 791, 1069
BstBI TTCGAA 1 cut(s) 471
BstDEI CTNAG 5 cut(s) 27, 35, 315, 612, 840
BstDSI CCRYGG 1 cut(s) 371
BstF5I GGATG 2 cut(s) 144, 684
BstFNI CGCG 1 cut(s) 780
BstH2I RGCGCY 1 cut(s) 619
BstHHI GCGC 3 cut(s) 259, 618, 780
BstKTI GATC 5 cut(s) 144, 380, 530, 658, 763
BstMAI GTCTC 4 cut(s) 182, 843, 855, 1174
BstMBI GATC 5 cut(s) 141, 377, 527, 655, 760
BstMCI CGRYCG 1 cut(s) 658
BstMWI GCNNNNNNNGC 1 cut(s) 365
BstNI CCWGG 1 cut(s) 1167
BstNSI RCATGY 1 cut(s) 802
BstSCI CCNGG 1 cut(s) 1165
BstSFI CTRYAG 1 cut(s) 919
BstSLI GKGCMC 1 cut(s) 507
BstUI CGCG 1 cut(s) 780
BstV1I GCAGC 2 cut(s) 241, 1173
BstX2I RGATCY 1 cut(s) 377
BstYI RGATCY 1 cut(s) 377
BsuRI GGCC 2 cut(s) 188, 449
BtgI CCRYGG 1 cut(s) 371
BtrI CACGTC 1 cut(s) 1063
BtsCI GGATG 2 cut(s) 144, 684
BtsIMutI CAGTG 4 cut(s) 39, 97, 848, 870
CaiI CAGNNNCTG 1 cut(s) 34
CfoI GCGC 3 cut(s) 259, 618, 780
Cfr13I GGNCC 4 cut(s) 187, 434, 448, 1031
Csp6I GTAC 6 cut(s) 490, 532, 802, 895, 916, 1098
CspCI CAANNNNNGTGG 2 cut(s) 859, 894
CviAII CATG 4 cut(s) 331, 681, 799, 930
CviJI RGCY 9 cut(s) 61, 188, 254, 359, 368, 449, 611, 927, 1086
CviKI_1 RGCY 9 cut(s) 61, 188, 254, 359, 368, 449, 611, 927, 1086
CviQI GTAC 6 cut(s) 490, 532, 802, 895, 916, 1098
DdeI CTNAG 5 cut(s) 27, 35, 315, 612, 840
DpnI GATC 5 cut(s) 143, 379, 529, 657, 762
DpnII GATC 5 cut(s) 141, 377, 527, 655, 760
Eco24I GRGCYC 1 cut(s) 929
Eco31I GGTCTC 1 cut(s) 843
Eco32I GATATC 2 cut(s) 386, 646
Eco47I GGWCC 2 cut(s) 434, 1031
Eco57I CTGAAG 3 cut(s) 685, 894, 1072
Eco88I CYCGRG 1 cut(s) 134
EcoRI GAATTC 2 cut(s) 564, 988
EcoRII CCWGG 1 cut(s) 1165
EcoRV GATATC 2 cut(s) 386, 646
EcoT22I ATGCAT 1 cut(s) 686
EcoT38I GRGCYC 1 cut(s) 929
FaeI CATG 4 cut(s) 334, 684, 802, 933
FaqI GGGAC 1 cut(s) 447
FatI CATG 4 cut(s) 330, 680, 798, 929
FauI CCCGC 2 cut(s) 773, 813
FauNDI CATATG 1 cut(s) 969
Fnu4HI GCNGC 3 cut(s) 255, 619, 1187
FokI GGATG 2 cut(s) 151, 671
FriOI GRGCYC 1 cut(s) 929
Fsp4HI GCNGC 3 cut(s) 255, 619, 1187
FspBI CTAG 2 cut(s) 570, 1152
FspI TGCGCA 1 cut(s) 258
GlaI GCGC 3 cut(s) 258, 617, 779
GluI GCNGC 3 cut(s) 255, 619, 1187
GsaI CCCAGC 1 cut(s) 283
HaeII RGCGCY 1 cut(s) 619
HaeIII GGCC 2 cut(s) 188, 449
HapII CCGG 2 cut(s) 381, 545
HhaI GCGC 3 cut(s) 259, 618, 780
Hin1II CATG 4 cut(s) 334, 684, 802, 933
Hin6I GCGC 3 cut(s) 257, 616, 778
HinP1I GCGC 3 cut(s) 257, 616, 778
HinfI GANTC 4 cut(s) 31, 271, 511, 677
HpaII CCGG 2 cut(s) 381, 545
HphI GGTGA 3 cut(s) 74, 266, 1042
Hpy166II GTNNAC 5 cut(s) 505, 794, 1031, 1050, 1161
Hpy188I TCNGA 6 cut(s) 30, 36, 290, 306, 405, 676
Hpy188III TCNNGA 8 cut(s) 136, 381, 424, 575, 641, 850, 910, 1090
Hpy8I GTNNAC 5 cut(s) 505, 794, 1031, 1050, 1161
HpyAV CCTTC 5 cut(s) 160, 208, 424, 467, 647
HpyCH4III ACNGT 3 cut(s) 43, 791, 1069
HpyCH4IV ACGT 1 cut(s) 1062
HpyCH4V TGCA 7 cut(s) 325, 505, 684, 921, 983, 1196, 1210
HpyF10VI GCNNNNNNNGC 1 cut(s) 365
HpyF3I CTNAG 5 cut(s) 27, 35, 315, 612, 840
HpySE526I ACGT 1 cut(s) 1062
Hsp92II CATG 4 cut(s) 334, 684, 802, 933
HspAI GCGC 3 cut(s) 257, 616, 778
Kpn2I TCCGGA 1 cut(s) 380
KpnI GGTACC 1 cut(s) 1101
Kzo9I GATC 5 cut(s) 141, 377, 527, 655, 760
LmnI GCTCC 1 cut(s) 373
Lsp1109I GCAGC 2 cut(s) 241, 1173
LweI GCATC 3 cut(s) 487, 693, 1197
MaeI CTAG 2 cut(s) 570, 1152
MaeII ACGT 1 cut(s) 1062
MaeIII GTNAC 4 cut(s) 247, 272, 819, 1063
MalI GATC 5 cut(s) 143, 379, 529, 657, 762
MboI GATC 5 cut(s) 141, 377, 527, 655, 760
MboII GAAGA 5 cut(s) 207, 353, 460, 573, 659
MflI RGATCY 1 cut(s) 377
MhlI GDGCHC 3 cut(s) 41, 507, 929
MlyI GAGTC 2 cut(s) 280, 686
MmeI TCCRAC 3 cut(s) 97, 103, 205
Mph1103I ATGCAT 1 cut(s) 686
MroI TCCGGA 1 cut(s) 380
MroXI GAANNNNTTC 2 cut(s) 467, 1085
MseI TTAA 1 cut(s) 1071
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 2 cut(s) 381, 545
MspR9I CCNGG 1 cut(s) 1167
MvaI CCWGG 1 cut(s) 1167
MvnI CGCG 1 cut(s) 780
MwoI GCNNNNNNNGC 1 cut(s) 365
NdeI CATATG 1 cut(s) 969
NdeII GATC 5 cut(s) 141, 377, 527, 655, 760
NlaIII CATG 4 cut(s) 334, 684, 802, 933
NlaIV GGNNCC 5 cut(s) 369, 435, 450, 848, 1099
NmuCI GTSAC 4 cut(s) 247, 272, 819, 1063
NsbI TGCGCA 1 cut(s) 258
NsiI ATGCAT 1 cut(s) 686
NspI RCATGY 1 cut(s) 802
NspV TTCGAA 1 cut(s) 471
PciI ACATGT 1 cut(s) 798
PdmI GAANNNNTTC 2 cut(s) 467, 1085
PfeI GAWTC 2 cut(s) 31, 511
PkrI GCNGC 3 cut(s) 256, 620, 1188
Ple19I CGATCG 1 cut(s) 658
PleI GAGTC 2 cut(s) 279, 685
PpsI GAGTC 2 cut(s) 279, 685
PscI ACATGT 1 cut(s) 798
Psp6I CCWGG 1 cut(s) 1165
PspFI CCCAGC 1 cut(s) 279
PspGI CCWGG 1 cut(s) 1165
PspN4I GGNNCC 5 cut(s) 369, 435, 450, 848, 1099
PspPI GGNCC 4 cut(s) 187, 434, 448, 1031
PsrI GAACNNNNNNTAC 1 cut(s) 36
PstI CTGCAG 1 cut(s) 923
PstNI CAGNNNCTG 1 cut(s) 34
PsuI RGATCY 1 cut(s) 377
PvuI CGATCG 1 cut(s) 658
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 6 cut(s) 491, 533, 803, 896, 917, 1099
RsaNI GTAC 6 cut(s) 490, 532, 802, 895, 916, 1098
SaqAI TTAA 1 cut(s) 1071
SatI GCNGC 3 cut(s) 255, 619, 1187
Sau3AI GATC 5 cut(s) 141, 377, 527, 655, 760
Sau96I GGNCC 4 cut(s) 187, 434, 448, 1031
ScaI AGTACT 2 cut(s) 896, 917
SchI GAGTC 2 cut(s) 280, 686
ScrFI CCNGG 1 cut(s) 1167
SduI GDGCHC 3 cut(s) 41, 507, 929
SfaNI GCATC 3 cut(s) 487, 693, 1197
SfcI CTRYAG 1 cut(s) 919
SfuI TTCGAA 1 cut(s) 471
SinI GGWCC 2 cut(s) 434, 1031
SsiI CCGC 5 cut(s) 619, 780, 806, 1034, 1189
SspMI CTAG 2 cut(s) 570, 1152
StyD4I CCNGG 1 cut(s) 1165
TaaI ACNGT 3 cut(s) 43, 791, 1069
TaiI ACGT 1 cut(s) 1065
TaqI TCGA 6 cut(s) 226, 397, 471, 494, 509, 1022
TatI WGTACW 3 cut(s) 489, 894, 915
TauI GCSGC 1 cut(s) 621
TfiI GAWTC 2 cut(s) 31, 511
Tru1I TTAA 1 cut(s) 1071
Tru9I TTAA 1 cut(s) 1071
TscAI CASTG 4 cut(s) 46, 97, 848, 877
TseFI GTSAC 4 cut(s) 247, 272, 819, 1063
TseI GCWGC 2 cut(s) 254, 1186
Tsp45I GTSAC 4 cut(s) 247, 272, 819, 1063
TspDTI ATGAA 6 cut(s) 17, 207, 347, 354, 956, 986
TspGWI ACGGA 2 cut(s) 388, 504
TspRI CASTG 4 cut(s) 46, 97, 848, 877
VneI GTGCAC 1 cut(s) 503
VpaK11BI GGWCC 2 cut(s) 434, 1031
XapI RAATTY 6 cut(s) 47, 299, 564, 727, 974, 988
XceI RCATGY 1 cut(s) 802
XmnI GAANNNNTTC 2 cut(s) 467, 1085
XspI CTAG 2 cut(s) 570, 1152
ZrmI AGTACT 2 cut(s) 896, 917
Zsp2I ATGCAT 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.