Rroxscaffold_5G00375960

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001447
Physical Location & Seq
Reverse (-)
56745447 .. 56747756
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375960.1

Sequence Viewer

Length: 1128 bp
ATGAAGGGTAGTGGTGGTACTACTCAGAATCTGAGCACTGTAAAATTTTTTCCAAAGCTCATCAATGTCCAACTCCATCACCTCATCAGTGTCCTCTCTTATTTCCTCTTATTTGGTGTTGGAATGACCCTCGGGATGGTCCTCACCTTCTATCTCAAAGACTTTTCTTTCAGTCTCCAACTGGCCCAATTCTCCTTCTCAACCTCACCATCTTCATCAAATCGAAACACGATACTGCCAAGTGTGACAGCTGCGCAAACCAATTTGAGTCACCCCCAGCACATCGGATTGGAGAAATTTCTGAAACCCCCTAAGATTTTGCACGACATGAATGATGAAGAATTGCTATGGAGAGCTTCTATGGCTCCTCGGAGATCCGACTATCCATTCCATCGAGTTCCGAAAGTTGCCTTCTTGTTCTTGACAAAGGGACCAGTGTTTCTGGCCCCATTGTGGGAGAAGTTCTTCAAAGGGCATCAAGGTTTGTACTCGATATATGTGCACTCGAATCCGTCTTACAATGGATCGTACCCCGAAAGTCCGGTGTTTCAAGGCAGAAGAATTCCTAGTCAGGAAGTGGAATGGGGAAATGTGAACATGATTGAGGCTGAGCGCCGCCTATTGGCAAATGCCCTTCTTGATATCTCAAACGATCGCTTTGTTCTTCTCTCGGAGTCATGCATCCCCCTCCACAACTTCCGCACCATCTACTACTTCCTTGTAAATTCGAAGCAGACATTTGTTCAGGTCTATGACGATCCAAGTAGTGTTGGGCGCGGGAGATACAATTCACACATGTATCCGCAAATCTCATTGTCACAATGGAGGAAAGGGTCTCAGTGGTTCCAGATAGATAGAGACATTGCCACTGAAGTTGTCGCAGACAAAAAGTACTTCCCACTTTTCCAGAAGTACTGCAGGGGCTCATGCTACGCAGACGAGCATTACTTCCCAACATTTGTTCATATGAAATTTTCTGCAAAGAATTCAAATAAAACCTTGACTTGGTATGACTTTTCGAGGGTGGACCGCACCCTGCCCAGTTCACCAGAACAGACGAAGTTCACTCCTGGCACTTTGGAGAGACTGCTGCGGTTTGCACCAAAATTGATGCAGTTCAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

375

Amino Acids

43.4

Weight (kDa)

9.58

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 136 - 343 2.1e-74 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 255
AccII CGCG 1 cut(s) 777
AciI CCGC 6 cut(s) 616, 700, 777, 803, 1030, 1093
AclWI GGATC 3 cut(s) 369, 532, 752
AcsI RAATTY 6 cut(s) 44, 296, 561, 724, 971, 985
AcuI CTGAAG 1 cut(s) 891
AfaI GTAC 5 cut(s) 19, 488, 530, 893, 914
AfiI CCNNNNNNNGG 5 cut(s) 136, 453, 454, 622, 1005
AflIII ACRYGT 1 cut(s) 795
AgsI TTSAA 4 cut(s) 469, 551, 990, 1120
AjnI CCWGG 1 cut(s) 1069
AluBI AGCT 3 cut(s) 58, 251, 356
AluI AGCT 3 cut(s) 58, 251, 356
Alw21I GWGCWC 2 cut(s) 38, 504
Alw26I GTCTC 4 cut(s) 179, 840, 852, 1078
Alw44I GTGCAC 1 cut(s) 500
AlwI GGATC 3 cut(s) 369, 532, 752
AlwNI CAGNNNCTG 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 2 cut(s) 183, 444
ApaLI GTGCAC 1 cut(s) 500
ApeKI GCWGC 2 cut(s) 251, 1090
ApoI RAATTY 6 cut(s) 44, 296, 561, 724, 971, 985
Asp700I GAANNNNTTC 1 cut(s) 464
AspLEI GCGC 3 cut(s) 256, 615, 777
AspS9I GGNCC 5 cut(s) 139, 184, 431, 445, 1027
AsuHPI GGTGA 5 cut(s) 71, 136, 198, 263, 1038
AsuII TTCGAA 1 cut(s) 728
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 3 cut(s) 139, 431, 1027
BaeGI GKGCMC 1 cut(s) 504
BaeI ACNNNNGTAYC 2 cut(s) 782, 815
BanII GRGCYC 1 cut(s) 926
Bbv12I GWGCWC 2 cut(s) 38, 504
BbvI GCAGC 2 cut(s) 238, 1077
BccI CCATC 5 cut(s) 84, 130, 217, 399, 713
BcgI CGANNNNNNTGC 2 cut(s) 481, 515
BciT130I CCWGG 1 cut(s) 1071
BciVI GTATCC 1 cut(s) 810
BcoDI GTCTC 4 cut(s) 179, 840, 852, 1078
BfaI CTAG 1 cut(s) 567
BfmI CTRYAG 1 cut(s) 916
BfoI RGCGCY 1 cut(s) 616
BfuI GTATCC 1 cut(s) 810
BisI GCNGC 3 cut(s) 252, 616, 1091
BlpI GCTNAGC 1 cut(s) 609
BlsI GCNGC 3 cut(s) 253, 617, 1092
BmcAI AGTACT 2 cut(s) 893, 914
Bme1390I CCNGG 1 cut(s) 1071
Bme18I GGWCC 3 cut(s) 139, 431, 1027
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 5 cut(s) 139, 184, 431, 445, 1027
BmiI GGNNCC 4 cut(s) 366, 432, 447, 845
BmrFI CCNGG 1 cut(s) 1071
BmrI ACTGGG 1 cut(s) 1035
BmsI GCATC 3 cut(s) 484, 690, 1101
BmuI ACTGGG 1 cut(s) 1035
Bpu1102I GCTNAGC 1 cut(s) 609
Bpu14I TTCGAA 1 cut(s) 728
BsaI GGTCTC 1 cut(s) 840
BsaJI CCNNGG 2 cut(s) 130, 368
BsaWI WCCGGW 1 cut(s) 541
Bsc4I CCNNNNNNNGG 5 cut(s) 136, 453, 454, 622, 1005
Bse1I ACTGG 3 cut(s) 186, 434, 1041
Bse3DI GCAATG 1 cut(s) 861
BseBI CCWGG 1 cut(s) 1071
BseDI CCNNGG 2 cut(s) 130, 368
BseGI GGATG 2 cut(s) 141, 681
BseLI CCNNNNNNNGG 5 cut(s) 136, 453, 454, 622, 1005
BseMI GCAATG 1 cut(s) 861
BseMII CTCAG 4 cut(s) 23, 38, 600, 851
BseNI ACTGG 3 cut(s) 186, 434, 1041
BseRI GAGGAG 1 cut(s) 357
BseSI GKGCMC 1 cut(s) 504
BseXI GCAGC 2 cut(s) 238, 1077
BseYI CCCAGC 1 cut(s) 276
Bsh1236I CGCG 1 cut(s) 777
Bsh1285I CGRYCG 1 cut(s) 655
BshFI GGCC 2 cut(s) 185, 446
BsiEI CGRYCG 1 cut(s) 655
BsiHKAI GWGCWC 2 cut(s) 38, 504
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 1 cut(s) 542
BslFI GGGAC 1 cut(s) 444
BslI CCNNNNNNNGG 5 cut(s) 136, 453, 454, 622, 1005
BsmAI GTCTC 4 cut(s) 179, 840, 852, 1078
BsmFI GGGAC 1 cut(s) 444
BsnI GGCC 2 cut(s) 185, 446
Bso31I GGTCTC 1 cut(s) 840
BsoBI CYCGRG 1 cut(s) 131
Bsp119I TTCGAA 1 cut(s) 728
Bsp1286I GDGCHC 3 cut(s) 38, 504, 926
Bsp143I GATC 4 cut(s) 374, 524, 652, 757
Bsp1720I GCTNAGC 1 cut(s) 609
BspACI CCGC 6 cut(s) 616, 700, 777, 803, 1030, 1093
BspANI GGCC 2 cut(s) 185, 446
BspCNI CTCAG 4 cut(s) 24, 37, 601, 850
BspFNI CGCG 1 cut(s) 777
BspLI GGNNCC 4 cut(s) 366, 432, 447, 845
BspMAI CTGCAG 1 cut(s) 920
BspPI GGATC 3 cut(s) 369, 532, 752
BspT104I TTCGAA 1 cut(s) 728
BspTNI GGTCTC 1 cut(s) 840
BsrDI GCAATG 1 cut(s) 861
BsrI ACTGG 3 cut(s) 186, 434, 1041
BssECI CCNNGG 2 cut(s) 130, 368
BssMI GATC 4 cut(s) 374, 524, 652, 757
Bst2UI CCWGG 1 cut(s) 1071
Bst4CI ACNGT 1 cut(s) 40
BstBI TTCGAA 1 cut(s) 728
BstDEI CTNAG 5 cut(s) 24, 32, 312, 609, 837
BstF5I GGATG 2 cut(s) 141, 681
BstFNI CGCG 1 cut(s) 777
BstH2I RGCGCY 1 cut(s) 616
BstHHI GCGC 3 cut(s) 256, 615, 777
BstKTI GATC 4 cut(s) 377, 527, 655, 760
BstMAI GTCTC 4 cut(s) 179, 840, 852, 1078
BstMBI GATC 4 cut(s) 374, 524, 652, 757
BstMCI CGRYCG 1 cut(s) 655
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNI CCWGG 1 cut(s) 1071
BstNSI RCATGY 1 cut(s) 799
BstSCI CCNGG 1 cut(s) 1069
BstSFI CTRYAG 1 cut(s) 916
BstSLI GKGCMC 1 cut(s) 504
BstUI CGCG 1 cut(s) 777
BstV1I GCAGC 2 cut(s) 238, 1077
BstX2I RGATCY 1 cut(s) 374
BstYI RGATCY 1 cut(s) 374
BsuI GTATCC 1 cut(s) 810
BsuRI GGCC 2 cut(s) 185, 446
BtsCI GGATG 2 cut(s) 141, 681
BtsIMutI CAGTG 5 cut(s) 36, 94, 441, 845, 867
CaiI CAGNNNCTG 1 cut(s) 31
CfoI GCGC 3 cut(s) 256, 615, 777
Cfr13I GGNCC 5 cut(s) 139, 184, 431, 445, 1027
Csp6I GTAC 5 cut(s) 18, 487, 529, 892, 913
CspCI CAANNNNNGTGG 2 cut(s) 856, 891
CviAII CATG 5 cut(s) 328, 598, 678, 796, 927
CviJI RGCY 8 cut(s) 58, 185, 251, 356, 365, 446, 608, 924
CviKI_1 RGCY 8 cut(s) 58, 185, 251, 356, 365, 446, 608, 924
CviQI GTAC 5 cut(s) 18, 487, 529, 892, 913
DdeI CTNAG 5 cut(s) 24, 32, 312, 609, 837
DpnI GATC 4 cut(s) 376, 526, 654, 759
DpnII GATC 4 cut(s) 374, 524, 652, 757
Eco24I GRGCYC 1 cut(s) 926
Eco31I GGTCTC 1 cut(s) 840
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 3 cut(s) 139, 431, 1027
Eco57I CTGAAG 1 cut(s) 891
Eco88I CYCGRG 1 cut(s) 131
EcoRI GAATTC 2 cut(s) 561, 985
EcoRII CCWGG 1 cut(s) 1069
EcoRV GATATC 1 cut(s) 643
EcoT22I ATGCAT 1 cut(s) 683
EcoT38I GRGCYC 1 cut(s) 926
FaeI CATG 5 cut(s) 331, 601, 681, 799, 930
FaqI GGGAC 1 cut(s) 444
FatI CATG 5 cut(s) 327, 597, 677, 795, 926
FauI CCCGC 1 cut(s) 770
FauNDI CATATG 1 cut(s) 966
Fnu4HI GCNGC 3 cut(s) 252, 616, 1091
FokI GGATG 2 cut(s) 148, 668
FriOI GRGCYC 1 cut(s) 926
Fsp4HI GCNGC 3 cut(s) 252, 616, 1091
FspBI CTAG 1 cut(s) 567
FspI TGCGCA 1 cut(s) 255
GlaI GCGC 3 cut(s) 255, 614, 776
GluI GCNGC 3 cut(s) 252, 616, 1091
GsaI CCCAGC 1 cut(s) 280
HaeII RGCGCY 1 cut(s) 616
HaeIII GGCC 2 cut(s) 185, 446
HapII CCGG 1 cut(s) 542
HhaI GCGC 3 cut(s) 256, 615, 777
Hin1II CATG 5 cut(s) 331, 601, 681, 799, 930
Hin6I GCGC 3 cut(s) 254, 613, 775
HinP1I GCGC 3 cut(s) 254, 613, 775
HinfI GANTC 4 cut(s) 28, 268, 508, 674
HpaII CCGG 1 cut(s) 542
HphI GGTGA 5 cut(s) 71, 136, 198, 263, 1038
Hpy166II GTNNAC 5 cut(s) 502, 595, 1027, 1046, 1065
Hpy188I TCNGA 8 cut(s) 27, 33, 287, 303, 372, 379, 402, 673
Hpy188III TCNNGA 6 cut(s) 133, 421, 572, 638, 847, 907
Hpy8I GTNNAC 5 cut(s) 502, 595, 1027, 1046, 1065
HpyAV CCTTC 4 cut(s) 157, 205, 421, 644
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4V TGCA 7 cut(s) 322, 502, 681, 918, 980, 1100, 1114
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 5 cut(s) 24, 32, 312, 609, 837
Hsp92II CATG 5 cut(s) 331, 601, 681, 799, 930
HspAI GCGC 3 cut(s) 254, 613, 775
Kzo9I GATC 4 cut(s) 374, 524, 652, 757
LmnI GCTCC 1 cut(s) 370
Lsp1109I GCAGC 2 cut(s) 238, 1077
LweI GCATC 3 cut(s) 484, 690, 1101
MaeI CTAG 1 cut(s) 567
MaeIII GTNAC 3 cut(s) 244, 269, 816
MalI GATC 4 cut(s) 376, 526, 654, 759
MboI GATC 4 cut(s) 374, 524, 652, 757
MboII GAAGA 5 cut(s) 204, 350, 457, 570, 656
MflI RGATCY 1 cut(s) 374
MhlI GDGCHC 3 cut(s) 38, 504, 926
MlyI GAGTC 2 cut(s) 277, 683
MmeI TCCRAC 4 cut(s) 94, 100, 202, 402
Mph1103I ATGCAT 1 cut(s) 683
MroXI GAANNNNTTC 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 251
MspI CCGG 1 cut(s) 542
MspR9I CCNGG 1 cut(s) 1071
MvaI CCWGG 1 cut(s) 1071
MvnI CGCG 1 cut(s) 777
MwoI GCNNNNNNNGC 1 cut(s) 362
NdeI CATATG 1 cut(s) 966
NdeII GATC 4 cut(s) 374, 524, 652, 757
NlaIII CATG 5 cut(s) 331, 601, 681, 799, 930
NlaIV GGNNCC 4 cut(s) 366, 432, 447, 845
NmuCI GTSAC 3 cut(s) 244, 269, 816
NsbI TGCGCA 1 cut(s) 255
NsiI ATGCAT 1 cut(s) 683
NspI RCATGY 1 cut(s) 799
NspV TTCGAA 1 cut(s) 728
PciI ACATGT 1 cut(s) 795
PdmI GAANNNNTTC 1 cut(s) 464
PfeI GAWTC 2 cut(s) 28, 508
PkrI GCNGC 3 cut(s) 253, 617, 1092
Ple19I CGATCG 1 cut(s) 655
PleI GAGTC 2 cut(s) 276, 682
PpsI GAGTC 2 cut(s) 276, 682
PscI ACATGT 1 cut(s) 795
Psp6I CCWGG 1 cut(s) 1069
PspFI CCCAGC 1 cut(s) 276
PspGI CCWGG 1 cut(s) 1069
PspN4I GGNNCC 4 cut(s) 366, 432, 447, 845
PspPI GGNCC 5 cut(s) 139, 184, 431, 445, 1027
PstI CTGCAG 1 cut(s) 920
PstNI CAGNNNCTG 1 cut(s) 31
PsuI RGATCY 1 cut(s) 374
PvuI CGATCG 1 cut(s) 655
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 5 cut(s) 19, 488, 530, 893, 914
RsaNI GTAC 5 cut(s) 18, 487, 529, 892, 913
SatI GCNGC 3 cut(s) 252, 616, 1091
Sau3AI GATC 4 cut(s) 374, 524, 652, 757
Sau96I GGNCC 5 cut(s) 139, 184, 431, 445, 1027
ScaI AGTACT 2 cut(s) 893, 914
SchI GAGTC 2 cut(s) 277, 683
ScrFI CCNGG 1 cut(s) 1071
SduI GDGCHC 3 cut(s) 38, 504, 926
SetI ASST 9 cut(s) 60, 84, 149, 206, 253, 358, 484, 750, 1001
SfaNI GCATC 3 cut(s) 484, 690, 1101
SfcI CTRYAG 1 cut(s) 916
SfuI TTCGAA 1 cut(s) 728
SinI GGWCC 3 cut(s) 139, 431, 1027
SsiI CCGC 6 cut(s) 616, 700, 777, 803, 1030, 1093
SspMI CTAG 1 cut(s) 567
StyD4I CCNGG 1 cut(s) 1069
TaaI ACNGT 1 cut(s) 40
TaqI TCGA 6 cut(s) 223, 394, 491, 506, 728, 1019
TatI WGTACW 3 cut(s) 486, 891, 912
TauI GCSGC 1 cut(s) 618
TfiI GAWTC 2 cut(s) 28, 508
TscAI CASTG 5 cut(s) 43, 94, 441, 845, 874
TseFI GTSAC 3 cut(s) 244, 269, 816
TseI GCWGC 2 cut(s) 251, 1090
Tsp45I GTSAC 3 cut(s) 244, 269, 816
TspDTI ATGAA 6 cut(s) 17, 204, 344, 351, 953, 983
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 5 cut(s) 43, 94, 441, 845, 874
VneI GTGCAC 1 cut(s) 500
VpaK11BI GGWCC 3 cut(s) 139, 431, 1027
XapI RAATTY 6 cut(s) 44, 296, 561, 724, 971, 985
XceI RCATGY 1 cut(s) 799
XmnI GAANNNNTTC 1 cut(s) 464
XspI CTAG 1 cut(s) 567
ZrmI AGTACT 2 cut(s) 893, 914
Zsp2I ATGCAT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.