Rw4G029170

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001447
Physical Location & Seq
Reverse (-)
55867955 .. 55872545
4591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029170.1

Sequence Viewer

Length: 1206 bp
ATGATCCTTACCTTCTATCTCAAAGACTTTTCTTTCAGTCTCCAACTGGCCCAAATCTCCTTCTCAACCTCATCATCTTCATCTAATCGAAACACGATACTGCCAAGTGTGACCGCTGCGCAAACCAATTTGAGTCACCCCCAGCACATCGGATTGGAGAAATTTCTGAAACCCCCTAAGATTTTGCACGACATGAATGATGAAGAATTGCTATGGAGAGCTTCTATGGCTCCACGGAGATCCGGATATCCATTCCATCGAGTTCCGAAAGTTGCCTTCTTGTTCTTGACAAAGGGGCCAGTTTTTCTGGCCCCATTGTGGGAGAAGTTCTTCAAAGGGCATCAAGGCTTGTACTCGATATATGTGCACTCGAATCCGTCTTACAATGGATCGTACCCCGAAAGTCCGGTGTTTCAAGGCAGAAGAATTCCTAGTCAGGAAGTGGAATGGGGAAAGGTGAATATGATTGAGGCTGAGCGCCGCCTATTGGCAAATGCCCTTCTTGATATCTCAAACGATCGCTTTGTTCTTCTCTCGGAGTCATGCATCCCCCTCCACAACTTCAGCACCATCTACCACTTCCTTGTAAATTCAAAACAGACATTTGTTCAGGTCTATGACGATCCAAGTAGTGTTGGGCGTGGGAGATACAATTCACACATGTATCCGCAAATCTCATTGTCACAATGGAGGAAAGGGTCTCAGTGGTTCCAGATAGATAGAGACATTGCCACTGAAGTTGTCGCAGACAAAAAGTACTTCCCACTTTTCCAGAAGTACTGCAGGGGCTCATGCTACGCAGACGAGCATTACTTCCCAACATTTGTTCATATGAAATTTTCTGCAAAGAATTCAAATAAAACCTTGACTTGGTATGACTTTTCGAGGGGCTATTGCTTTGCAGATGAGCATTACTTGCCAACATTTATGAACATAAGATTTCCGAAAAAGAATTCAAACAGGACTGTGACCTGGGTTGATTGGTCTAGGGGCGGACCTCATCCTGTCGAGTACGCGAGCACAGATGTCACGGTTGAGCTTCTGAGTACTCTGAGGAAGGGTAACGGCTGGTGTGATTACAATGGAAGGGGCACAGATGTTTGTTTCTTGTTCGCCCGGAAGTTCTCGCCAAATTCTTTGGATAAGCTGCTGAGCATTGCACCACAGTTAATGCAATTCAACACAGACATTGATAAAGAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

401

Amino Acids

46.49

Weight (kDa)

9.09

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 91 - 297 1.2e-74 Core-2/I-Branching enzyme
Branch PF02485 297 - 351 6.7e-13 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 120
AccII CGCG 1 cut(s) 1016
AccIII TCCGGA 1 cut(s) 242
AciI CCGC 4 cut(s) 114, 481, 668, 993
AclWI GGATC 3 cut(s) 234, 397, 617
AcsI RAATTY 7 cut(s) 161, 426, 589, 836, 850, 952, 1132
AcuI CTGAAG 2 cut(s) 547, 756
AfaI GTAC 7 cut(s) 353, 395, 758, 779, 1013, 1048, 1202
AfiI CCNNNNNNNGG 4 cut(s) 318, 319, 487, 870
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 6 cut(s) 334, 416, 594, 855, 957, 1180
AjnI CCWGG 1 cut(s) 971
AluBI AGCT 3 cut(s) 221, 1039, 1147
AluI AGCT 3 cut(s) 221, 1039, 1147
Alw21I GWGCWC 2 cut(s) 369, 1022
Alw26I GTCTC 3 cut(s) 44, 705, 717
Alw44I GTGCAC 1 cut(s) 365
AlwI GGATC 3 cut(s) 234, 397, 617
Aor13HI TCCGGA 1 cut(s) 242
AoxI GGCC 3 cut(s) 48, 296, 309
ApaLI GTGCAC 1 cut(s) 365
ApeKI GCWGC 2 cut(s) 116, 1147
ApoI RAATTY 7 cut(s) 161, 426, 589, 836, 850, 952, 1132
Asp700I GAANNNNTTC 1 cut(s) 329
AspLEI GCGC 2 cut(s) 121, 480
AspS9I GGNCC 4 cut(s) 49, 296, 310, 995
AsuC2I CCSGG 1 cut(s) 1117
AsuHPI GGTGA 2 cut(s) 128, 469
AvaII GGWCC 1 cut(s) 995
BaeGI GKGCMC 2 cut(s) 369, 1094
BaeI ACNNNNGTAYC 2 cut(s) 647, 680
BanII GRGCYC 1 cut(s) 791
Bbv12I GWGCWC 2 cut(s) 369, 1022
BbvI GCAGC 2 cut(s) 103, 1134
BccI CCATC 2 cut(s) 264, 578
BceAI ACGGC 1 cut(s) 1081
BcgI CGANNNNNNTGC 2 cut(s) 346, 380
BciT130I CCWGG 1 cut(s) 973
BciVI GTATCC 1 cut(s) 675
BcnI CCSGG 1 cut(s) 1117
BcoDI GTCTC 3 cut(s) 44, 705, 717
BfaI CTAG 3 cut(s) 432, 987, 1204
BfmI CTRYAG 1 cut(s) 781
BfoI RGCGCY 1 cut(s) 481
BfuI GTATCC 1 cut(s) 675
BisI GCNGC 3 cut(s) 117, 481, 1148
BlpI GCTNAGC 2 cut(s) 474, 1151
BlsI GCNGC 3 cut(s) 118, 482, 1149
BmcAI AGTACT 4 cut(s) 758, 779, 1048, 1202
Bme1390I CCNGG 2 cut(s) 973, 1117
Bme18I GGWCC 1 cut(s) 995
BmgT120I GGNCC 4 cut(s) 49, 296, 310, 995
BmiI GGNNCC 4 cut(s) 231, 297, 312, 710
BmrFI CCNGG 2 cut(s) 973, 1117
BmsI GCATC 2 cut(s) 349, 555
Bpu1102I GCTNAGC 2 cut(s) 474, 1151
BpuMI CCSGG 1 cut(s) 1117
BsaI GGTCTC 1 cut(s) 705
BsaJI CCNNGG 2 cut(s) 233, 972
BsaWI WCCGGW 2 cut(s) 242, 406
Bsc4I CCNNNNNNNGG 4 cut(s) 318, 319, 487, 870
Bse1I ACTGG 2 cut(s) 51, 299
Bse3DI GCAATG 2 cut(s) 726, 1155
BseAI TCCGGA 1 cut(s) 242
BseBI CCWGG 1 cut(s) 973
BseDI CCNNGG 2 cut(s) 233, 972
BseGI GGATG 2 cut(s) 546, 1000
BseLI CCNNNNNNNGG 4 cut(s) 318, 319, 487, 870
BseMI GCAATG 2 cut(s) 726, 1155
BseMII CTCAG 5 cut(s) 465, 716, 1034, 1043, 1142
BseNI ACTGG 2 cut(s) 51, 299
BseSI GKGCMC 2 cut(s) 369, 1094
BseXI GCAGC 2 cut(s) 103, 1134
BseYI CCCAGC 1 cut(s) 141
Bsh1236I CGCG 1 cut(s) 1016
Bsh1285I CGRYCG 1 cut(s) 520
BshFI GGCC 3 cut(s) 50, 298, 311
BsiEI CGRYCG 1 cut(s) 520
BsiHKAI GWGCWC 2 cut(s) 369, 1022
BsiSI CCGG 3 cut(s) 243, 407, 1117
BslI CCNNNNNNNGG 4 cut(s) 318, 319, 487, 870
BsmAI GTCTC 3 cut(s) 44, 705, 717
BsnI GGCC 3 cut(s) 50, 298, 311
Bso31I GGTCTC 1 cut(s) 705
Bsp1286I GDGCHC 4 cut(s) 369, 791, 1022, 1094
Bsp13I TCCGGA 1 cut(s) 242
Bsp143I GATC 5 cut(s) 3, 239, 389, 517, 622
Bsp1720I GCTNAGC 2 cut(s) 474, 1151
BspACI CCGC 4 cut(s) 114, 481, 668, 993
BspANI GGCC 3 cut(s) 50, 298, 311
BspCNI CTCAG 5 cut(s) 466, 715, 1035, 1044, 1143
BspEI TCCGGA 1 cut(s) 242
BspFNI CGCG 1 cut(s) 1016
BspLI GGNNCC 4 cut(s) 231, 297, 312, 710
BspMAI CTGCAG 1 cut(s) 785
BspPI GGATC 3 cut(s) 234, 397, 617
BspTNI GGTCTC 1 cut(s) 705
BsrDI GCAATG 2 cut(s) 726, 1155
BsrI ACTGG 2 cut(s) 51, 299
BssECI CCNNGG 2 cut(s) 233, 972
BssMI GATC 5 cut(s) 3, 239, 389, 517, 622
Bst2UI CCWGG 1 cut(s) 973
Bst4CI ACNGT 3 cut(s) 967, 1033, 1167
BstAPI GCANNNNNTGC 1 cut(s) 916
BstC8I GCNNGC 1 cut(s) 1018
BstDEI CTNAG 6 cut(s) 177, 474, 702, 1043, 1052, 1151
BstDSI CCRYGG 1 cut(s) 233
BstF5I GGATG 2 cut(s) 546, 1000
BstFNI CGCG 1 cut(s) 1016
BstH2I RGCGCY 1 cut(s) 481
BstHHI GCGC 2 cut(s) 121, 480
BstKTI GATC 5 cut(s) 6, 242, 392, 520, 625
BstMAI GTCTC 3 cut(s) 44, 705, 717
BstMBI GATC 5 cut(s) 3, 239, 389, 517, 622
BstMCI CGRYCG 1 cut(s) 520
BstMWI GCNNNNNNNGC 2 cut(s) 227, 916
BstNI CCWGG 1 cut(s) 973
BstNSI RCATGY 1 cut(s) 664
BstSCI CCNGG 2 cut(s) 971, 1115
BstSFI CTRYAG 1 cut(s) 781
BstSLI GKGCMC 2 cut(s) 369, 1094
BstUI CGCG 1 cut(s) 1016
BstV1I GCAGC 2 cut(s) 103, 1134
BstX2I RGATCY 1 cut(s) 239
BstYI RGATCY 1 cut(s) 239
BsuI GTATCC 1 cut(s) 675
BsuRI GGCC 3 cut(s) 50, 298, 311
BtgI CCRYGG 1 cut(s) 233
BtsCI GGATG 2 cut(s) 546, 1000
BtsIMutI CAGTG 2 cut(s) 710, 732
Cac8I GCNNGC 1 cut(s) 1018
CfoI GCGC 2 cut(s) 121, 480
Cfr13I GGNCC 4 cut(s) 49, 296, 310, 995
Csp6I GTAC 7 cut(s) 352, 394, 757, 778, 1012, 1047, 1201
CspCI CAANNNNNGTGG 2 cut(s) 721, 756
CviAII CATG 4 cut(s) 193, 543, 661, 792
CviQI GTAC 7 cut(s) 352, 394, 757, 778, 1012, 1047, 1201
DdeI CTNAG 6 cut(s) 177, 474, 702, 1043, 1052, 1151
DpnI GATC 5 cut(s) 5, 241, 391, 519, 624
DpnII GATC 5 cut(s) 3, 239, 389, 517, 622
EciI GGCGGA 1 cut(s) 1008
Eco24I GRGCYC 1 cut(s) 791
Eco31I GGTCTC 1 cut(s) 705
Eco32I GATATC 2 cut(s) 248, 508
Eco47I GGWCC 1 cut(s) 995
Eco57I CTGAAG 2 cut(s) 547, 756
EcoRI GAATTC 3 cut(s) 426, 850, 952
EcoRII CCWGG 1 cut(s) 971
EcoRV GATATC 2 cut(s) 248, 508
EcoT22I ATGCAT 1 cut(s) 548
EcoT38I GRGCYC 1 cut(s) 791
FaeI CATG 4 cut(s) 196, 546, 664, 795
FatI CATG 4 cut(s) 192, 542, 660, 791
FauNDI CATATG 1 cut(s) 831
Fnu4HI GCNGC 3 cut(s) 117, 481, 1148
FokI GGATG 2 cut(s) 533, 987
FriOI GRGCYC 1 cut(s) 791
Fsp4HI GCNGC 3 cut(s) 117, 481, 1148
FspBI CTAG 3 cut(s) 432, 987, 1204
FspI TGCGCA 1 cut(s) 120
GlaI GCGC 2 cut(s) 120, 479
GluI GCNGC 3 cut(s) 117, 481, 1148
GsaI CCCAGC 1 cut(s) 145
HaeII RGCGCY 1 cut(s) 481
HaeIII GGCC 3 cut(s) 50, 298, 311
HapII CCGG 3 cut(s) 243, 407, 1117
HhaI GCGC 2 cut(s) 121, 480
Hin1II CATG 4 cut(s) 196, 546, 664, 795
Hin6I GCGC 2 cut(s) 119, 478
HinP1I GCGC 2 cut(s) 119, 478
HinfI GANTC 3 cut(s) 133, 373, 539
HpaII CCGG 3 cut(s) 243, 407, 1117
HphI GGTGA 2 cut(s) 128, 469
Hpy166II GTNNAC 1 cut(s) 367
Hpy188I TCNGA 7 cut(s) 152, 168, 267, 538, 945, 1044, 1053
Hpy188III TCNNGA 6 cut(s) 243, 286, 437, 503, 712, 772
Hpy8I GTNNAC 1 cut(s) 367
HpyAV CCTTC 6 cut(s) 22, 70, 286, 509, 1051, 1080
HpyCH4III ACNGT 3 cut(s) 967, 1033, 1167
HpyCH4V TGCA 8 cut(s) 187, 367, 546, 783, 845, 902, 1160, 1174
HpyF10VI GCNNNNNNNGC 2 cut(s) 227, 916
HpyF3I CTNAG 6 cut(s) 177, 474, 702, 1043, 1052, 1151
Hsp92II CATG 4 cut(s) 196, 546, 664, 795
HspAI GCGC 2 cut(s) 119, 478
Kpn2I TCCGGA 1 cut(s) 242
Kzo9I GATC 5 cut(s) 3, 239, 389, 517, 622
LmnI GCTCC 1 cut(s) 235
Lsp1109I GCAGC 2 cut(s) 103, 1134
LweI GCATC 2 cut(s) 349, 555
MaeI CTAG 3 cut(s) 432, 987, 1204
MaeIII GTNAC 6 cut(s) 109, 134, 681, 967, 1027, 1061
MalI GATC 5 cut(s) 5, 241, 391, 519, 624
MboI GATC 5 cut(s) 3, 239, 389, 517, 622
MboII GAAGA 5 cut(s) 69, 215, 322, 435, 521
MflI RGATCY 1 cut(s) 239
MhlI GDGCHC 4 cut(s) 369, 791, 1022, 1094
MlyI GAGTC 2 cut(s) 142, 548
MmeI TCCRAC 1 cut(s) 67
MnlI CCTC 7 cut(s) 79, 463, 563, 684, 879, 1008, 1047
Mph1103I ATGCAT 1 cut(s) 548
MroI TCCGGA 1 cut(s) 242
MroXI GAANNNNTTC 1 cut(s) 329
MseI TTAA 1 cut(s) 1169
MspA1I CMGCKG 1 cut(s) 116
MspI CCGG 3 cut(s) 243, 407, 1117
MspR9I CCNGG 2 cut(s) 973, 1117
MvaI CCWGG 1 cut(s) 973
MvnI CGCG 1 cut(s) 1016
MwoI GCNNNNNNNGC 2 cut(s) 227, 916
NciI CCSGG 1 cut(s) 1117
NdeI CATATG 1 cut(s) 831
NdeII GATC 5 cut(s) 3, 239, 389, 517, 622
NlaIII CATG 4 cut(s) 196, 546, 664, 795
NlaIV GGNNCC 4 cut(s) 231, 297, 312, 710
NmuCI GTSAC 5 cut(s) 109, 134, 681, 967, 1027
NsbI TGCGCA 1 cut(s) 120
NsiI ATGCAT 1 cut(s) 548
NspI RCATGY 1 cut(s) 664
PciI ACATGT 1 cut(s) 660
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 1 cut(s) 373
PkrI GCNGC 3 cut(s) 118, 482, 1149
Ple19I CGATCG 1 cut(s) 520
PleI GAGTC 2 cut(s) 141, 547
PpsI GAGTC 2 cut(s) 141, 547
PscI ACATGT 1 cut(s) 660
Psp6I CCWGG 1 cut(s) 971
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 1 cut(s) 971
PspN4I GGNNCC 4 cut(s) 231, 297, 312, 710
PspPI GGNCC 4 cut(s) 49, 296, 310, 995
PstI CTGCAG 1 cut(s) 785
PsuI RGATCY 1 cut(s) 239
PvuI CGATCG 1 cut(s) 520
RsaI GTAC 7 cut(s) 353, 395, 758, 779, 1013, 1048, 1202
RsaNI GTAC 7 cut(s) 352, 394, 757, 778, 1012, 1047, 1201
SaqAI TTAA 1 cut(s) 1169
SatI GCNGC 3 cut(s) 117, 481, 1148
Sau3AI GATC 5 cut(s) 3, 239, 389, 517, 622
Sau96I GGNCC 4 cut(s) 49, 296, 310, 995
ScaI AGTACT 4 cut(s) 758, 779, 1048, 1202
SchI GAGTC 2 cut(s) 142, 548
ScrFI CCNGG 2 cut(s) 973, 1117
SduI GDGCHC 4 cut(s) 369, 791, 1022, 1094
SfaNI GCATC 2 cut(s) 349, 555
SfcI CTRYAG 1 cut(s) 781
SinI GGWCC 1 cut(s) 995
SsiI CCGC 4 cut(s) 114, 481, 668, 993
SspMI CTAG 3 cut(s) 432, 987, 1204
StyD4I CCNGG 2 cut(s) 971, 1115
TaaI ACNGT 3 cut(s) 967, 1033, 1167
TaqI TCGA 6 cut(s) 88, 259, 356, 371, 884, 1008
TatI WGTACW 5 cut(s) 351, 756, 777, 1046, 1200
TauI GCSGC 1 cut(s) 483
TfiI GAWTC 1 cut(s) 373
Tru1I TTAA 1 cut(s) 1169
Tru9I TTAA 1 cut(s) 1169
TscAI CASTG 2 cut(s) 710, 739
TseFI GTSAC 5 cut(s) 109, 134, 681, 967, 1027
TseI GCWGC 2 cut(s) 116, 1147
Tsp45I GTSAC 5 cut(s) 109, 134, 681, 967, 1027
TspDTI ATGAA 6 cut(s) 69, 209, 216, 818, 848, 944
TspGWI ACGGA 2 cut(s) 250, 366
TspRI CASTG 2 cut(s) 710, 739
VneI GTGCAC 1 cut(s) 365
VpaK11BI GGWCC 1 cut(s) 995
XapI RAATTY 7 cut(s) 161, 426, 589, 836, 850, 952, 1132
XceI RCATGY 1 cut(s) 664
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 3 cut(s) 432, 987, 1204
ZrmI AGTACT 4 cut(s) 758, 779, 1048, 1202
Zsp2I ATGCAT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.