Rh4DG336600

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001447
Physical Location & Seq
Reverse (-)
55978114 .. 55979147
1034 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG336600.1

Sequence Viewer

Length: 951 bp
ATGATGAAGAATTGCTATGGAGAGCTTCGACGGCTCCTCGGATTTCTGAATCCATTCCATCGAGTCCCAAAAGTTGCTTTCATGTTCTTGACAAAGGGACCTGTACATTTGGCCCCATTGTGGGAGAAGTTCTTCAAAGGGCATCAAGGCTTGTACTCAATTTATGTTCACTCAGATCCATCTTACAATGCATCATCACACCCAGGAAGTCCTCTTTTTTCAAGGCAGAAGAATTCCAAGTCAGGTCAGCTTTTTTGCTTAGTAAAATCATCCTCAATATTCATCAGCAGTTATTTCATTCTACTAATTTCATGTCCTGCAGAAAGTAAAATGGGCACAGGTGAACATGGTAATTGCGAATGCTCTTCTCGATATCTCAAACCAGCGGTACGGTTCGTTCTCCTCTCGGAAGCATGCATTCCCCTATACAACTTCTCCACTATCTACACTTACCTCCTAAACTCCCAAGAAACTTTTGTGGAGGTCTATGATGATCCAAGTTCAGTTGGACGCGGCCGATATAATTCAAGTTACTACCCAATATTGGATCAGTGGAGGAAAGGGTCACAGTGGGTTGAAATTGATAGAGACATTGCCATTGAAGTTGTCTCGGATCGGACATACTTGCCAGCCTTCCGGTTGGGCAACCGCTCTTGTTTTGCTGATGAACAATACTCGCCAACATTTGTGAACCTAAAATTTTCGGCAAAGAATTCAAACAGGAGTGTCAACTGGGTTAATTGGTCTACGCGTGGCCCTCACCCTGTCGAGTACAATAGCACATCGGCCGAGCTTCTGAACACCCTGCGCAATGGTAATGATCAGAGATGTTTATATAATCGAAGGAGAAGCAATGTTTGTTTCTTATTCGCCAGGAAGTTCCGGCCTTCTGCTTTGGATAGCCTTCTCAGGCTTGCCCCCAAGATCATGCACTTCAACACAGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

316

Amino Acids

36.3

Weight (kDa)

9.41

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 25 - 268 2.6e-50 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 809
AccBSI CCGCTC 1 cut(s) 651
AccI GTMKAC 1 cut(s) 746
AccII CGCG 2 cut(s) 513, 751
AciI CCGC 3 cut(s) 386, 513, 649
AclWI GGATC 4 cut(s) 170, 488, 555, 621
AcoI YGGCCR 2 cut(s) 514, 786
AcsI RAATTY 3 cut(s) 232, 698, 712
AfaI GTAC 4 cut(s) 105, 155, 390, 773
AfiI CCNNNNNNNGG 3 cut(s) 120, 121, 544
AflIII ACRYGT 1 cut(s) 749
AgsI TTSAA 7 cut(s) 136, 222, 528, 578, 602, 717, 937
AjnI CCWGG 2 cut(s) 202, 872
AluBI AGCT 3 cut(s) 25, 250, 793
AluI AGCT 3 cut(s) 25, 250, 793
Alw26I GTCTC 2 cut(s) 582, 613
AlwI GGATC 4 cut(s) 170, 488, 555, 621
AoxI GGCC 5 cut(s) 111, 514, 754, 786, 884
ApoI RAATTY 3 cut(s) 232, 698, 712
Asp700I GAANNNNTTC 2 cut(s) 53, 131
AspLEI GCGC 1 cut(s) 810
AspS9I GGNCC 3 cut(s) 98, 112, 755
AsuHPI GGTGA 2 cut(s) 353, 752
AvaII GGWCC 1 cut(s) 98
BaeGI GKGCMC 1 cut(s) 338
BccI CCATC 2 cut(s) 66, 187
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 2 cut(s) 204, 874
BclI TGATCA 1 cut(s) 820
BcoDI GTCTC 2 cut(s) 582, 613
BfmI CTRYAG 1 cut(s) 318
BisI GCNGC 1 cut(s) 514
BlsI GCNGC 1 cut(s) 515
Bme1390I CCNGG 2 cut(s) 204, 874
Bme18I GGWCC 1 cut(s) 98
BmgT120I GGNCC 3 cut(s) 98, 112, 755
BmiI GGNNCC 3 cut(s) 35, 99, 114
BmrFI CCNGG 2 cut(s) 204, 874
BmrI ACTGGG 1 cut(s) 742
BmsI GCATC 2 cut(s) 151, 200
BmuI ACTGGG 1 cut(s) 742
BsaJI CCNNGG 2 cut(s) 37, 202
BsaWI WCCGGW 1 cut(s) 636
BsaXI ACNNNNNCTCC 2 cut(s) 419, 449
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 121, 544
Bse1I ACTGG 1 cut(s) 737
Bse3DI GCAATG 3 cut(s) 591, 817, 859
BseBI CCWGG 2 cut(s) 204, 874
BseDI CCNNGG 2 cut(s) 37, 202
BseGI GGATG 1 cut(s) 269
BseLI CCNNNNNNNGG 3 cut(s) 120, 121, 544
BseMI GCAATG 3 cut(s) 591, 817, 859
BseMII CTCAG 2 cut(s) 186, 922
BseNI ACTGG 1 cut(s) 737
BseRI GAGGAG 2 cut(s) 26, 392
BseSI GKGCMC 1 cut(s) 338
BseX3I CGGCCG 2 cut(s) 514, 786
Bsh1236I CGCG 2 cut(s) 513, 751
Bsh1285I CGRYCG 2 cut(s) 517, 789
BshFI GGCC 5 cut(s) 113, 516, 756, 788, 886
BsiEI CGRYCG 2 cut(s) 517, 789
BsiSI CCGG 2 cut(s) 637, 883
BslFI GGGAC 2 cut(s) 50, 111
BslI CCNNNNNNNGG 3 cut(s) 120, 121, 544
BsmAI GTCTC 2 cut(s) 582, 613
BsmFI GGGAC 2 cut(s) 50, 111
BsmI GAATGC 2 cut(s) 365, 417
BsnI GGCC 5 cut(s) 113, 516, 756, 788, 886
Bsp1286I GDGCHC 1 cut(s) 338
Bsp1407I TGTACA 1 cut(s) 103
Bsp143I GATC 6 cut(s) 175, 493, 547, 613, 820, 924
BspACI CCGC 3 cut(s) 386, 513, 649
BspANI GGCC 5 cut(s) 113, 516, 756, 788, 886
BspCNI CTCAG 2 cut(s) 185, 921
BspFNI CGCG 2 cut(s) 513, 751
BspLI GGNNCC 3 cut(s) 35, 99, 114
BspMAI CTGCAG 1 cut(s) 322
BspPI GGATC 4 cut(s) 170, 488, 555, 621
BspQI GCTCTTC 1 cut(s) 370
BsrBI CCGCTC 1 cut(s) 651
BsrDI GCAATG 3 cut(s) 591, 817, 859
BsrGI TGTACA 1 cut(s) 103
BsrI ACTGG 1 cut(s) 737
BssECI CCNNGG 2 cut(s) 37, 202
BssMI GATC 6 cut(s) 175, 493, 547, 613, 820, 924
Bst2UI CCWGG 2 cut(s) 204, 874
Bst4CI ACNGT 2 cut(s) 393, 570
Bst6I CTCTTC 1 cut(s) 370
BstAUI TGTACA 1 cut(s) 103
BstC8I GCNNGC 3 cut(s) 415, 630, 915
BstDEI CTNAG 3 cut(s) 172, 259, 908
BstF5I GGATG 1 cut(s) 269
BstFNI CGCG 2 cut(s) 513, 751
BstHHI GCGC 1 cut(s) 810
BstKTI GATC 6 cut(s) 178, 496, 550, 616, 823, 927
BstMAI GTCTC 2 cut(s) 582, 613
BstMBI GATC 6 cut(s) 175, 493, 547, 613, 820, 924
BstMCI CGRYCG 2 cut(s) 517, 789
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstNI CCWGG 2 cut(s) 204, 874
BstNSI RCATGY 1 cut(s) 417
BstSCI CCNGG 2 cut(s) 202, 872
BstSFI CTRYAG 1 cut(s) 318
BstSLI GKGCMC 1 cut(s) 338
BstUI CGCG 2 cut(s) 513, 751
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BstZI CGGCCG 2 cut(s) 514, 786
BsuRI GGCC 5 cut(s) 113, 516, 756, 788, 886
BtsCI GGATG 1 cut(s) 269
BtsIMutI CAGTG 2 cut(s) 557, 575
Cac8I GCNNGC 3 cut(s) 415, 630, 915
CfoI GCGC 1 cut(s) 810
Cfr13I GGNCC 3 cut(s) 98, 112, 755
CseI GACGC 1 cut(s) 519
Csp6I GTAC 4 cut(s) 104, 154, 389, 772
CviAII CATG 5 cut(s) 82, 312, 347, 414, 928
CviQI GTAC 4 cut(s) 104, 154, 389, 772
DdeI CTNAG 3 cut(s) 172, 259, 908
DpnI GATC 6 cut(s) 177, 495, 549, 615, 822, 926
DpnII GATC 6 cut(s) 175, 493, 547, 613, 820, 924
EaeI YGGCCR 2 cut(s) 514, 786
EagI CGGCCG 2 cut(s) 514, 786
Eam1104I CTCTTC 1 cut(s) 370
EarI CTCTTC 1 cut(s) 370
EclXI CGGCCG 2 cut(s) 514, 786
Eco32I GATATC 1 cut(s) 374
Eco47I GGWCC 1 cut(s) 98
Eco52I CGGCCG 2 cut(s) 514, 786
EcoO109I RGGNCCY 1 cut(s) 98
EcoRI GAATTC 2 cut(s) 232, 712
EcoRII CCWGG 2 cut(s) 202, 872
EcoRV GATATC 1 cut(s) 374
EcoT22I ATGCAT 2 cut(s) 193, 419
FaeI CATG 5 cut(s) 85, 315, 350, 417, 931
FaqI GGGAC 2 cut(s) 50, 111
FatI CATG 5 cut(s) 81, 311, 346, 413, 927
FbaI TGATCA 1 cut(s) 820
FblI GTMKAC 1 cut(s) 746
Fnu4HI GCNGC 1 cut(s) 514
FokI GGATG 1 cut(s) 256
Fsp4HI GCNGC 1 cut(s) 514
FspI TGCGCA 1 cut(s) 809
GlaI GCGC 1 cut(s) 809
GluI GCNGC 1 cut(s) 514
HaeIII GGCC 5 cut(s) 113, 516, 756, 788, 886
HapII CCGG 2 cut(s) 637, 883
HgaI GACGC 1 cut(s) 519
HhaI GCGC 1 cut(s) 810
Hin1II CATG 5 cut(s) 85, 315, 350, 417, 931
Hin6I GCGC 1 cut(s) 808
HinP1I GCGC 1 cut(s) 808
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HinfI GANTC 3 cut(s) 49, 63, 944
HpaII CCGG 2 cut(s) 637, 883
HphI GGTGA 2 cut(s) 353, 752
Hpy166II GTNNAC 5 cut(s) 169, 344, 691, 730, 747
Hpy188I TCNGA 8 cut(s) 41, 48, 175, 409, 613, 618, 798, 825
Hpy188III TCNNGA 2 cut(s) 88, 369
Hpy8I GTNNAC 5 cut(s) 169, 344, 691, 730, 747
Hpy99I CGWCG 1 cut(s) 33
HpyAV CCTTC 4 cut(s) 643, 837, 897, 914
HpyCH4III ACNGT 2 cut(s) 393, 570
HpyCH4V TGCA 4 cut(s) 191, 320, 417, 931
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 3 cut(s) 172, 259, 908
Hsp92II CATG 5 cut(s) 85, 315, 350, 417, 931
HspAI GCGC 1 cut(s) 808
Ksp22I TGATCA 1 cut(s) 820
Kzo9I GATC 6 cut(s) 175, 493, 547, 613, 820, 924
LguI GCTCTTC 1 cut(s) 370
LmnI GCTCC 1 cut(s) 39
LweI GCATC 2 cut(s) 151, 200
MaeIII GTNAC 2 cut(s) 530, 564
MalI GATC 6 cut(s) 177, 495, 549, 615, 822, 926
MbiI CCGCTC 1 cut(s) 651
MboI GATC 6 cut(s) 175, 493, 547, 613, 820, 924
MboII GAAGA 4 cut(s) 19, 124, 241, 357
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 338
MluI ACGCGT 1 cut(s) 749
MlyI GAGTC 1 cut(s) 72
MmeI TCCRAC 1 cut(s) 487
MnlI CCTC 8 cut(s) 47, 222, 283, 413, 464, 475, 549, 768
Mph1103I ATGCAT 2 cut(s) 193, 419
MroXI GAANNNNTTC 2 cut(s) 53, 131
MseI TTAA 2 cut(s) 738, 949
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 2 cut(s) 637, 883
MspR9I CCNGG 2 cut(s) 204, 874
Mva1269I GAATGC 2 cut(s) 365, 417
MvaI CCWGG 2 cut(s) 204, 874
MvnI CGCG 2 cut(s) 513, 751
MwoI GCNNNNNNNGC 1 cut(s) 31
NdeII GATC 6 cut(s) 175, 493, 547, 613, 820, 924
NlaIII CATG 5 cut(s) 85, 315, 350, 417, 931
NlaIV GGNNCC 3 cut(s) 35, 99, 114
NmeAIII GCCGAG 1 cut(s) 814
NmuCI GTSAC 1 cut(s) 564
NsbI TGCGCA 1 cut(s) 809
NsiI ATGCAT 2 cut(s) 193, 419
NspI RCATGY 1 cut(s) 417
PaeI GCATGC 1 cut(s) 417
PciSI GCTCTTC 1 cut(s) 370
PctI GAATGC 2 cut(s) 365, 417
PdmI GAANNNNTTC 2 cut(s) 53, 131
PfeI GAWTC 2 cut(s) 49, 944
PkrI GCNGC 1 cut(s) 515
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
PpuMI RGGWCCY 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 202, 872
PspGI CCWGG 2 cut(s) 202, 872
PspN4I GGNNCC 3 cut(s) 35, 99, 114
PspPI GGNCC 3 cut(s) 98, 112, 755
PspPPI RGGWCCY 1 cut(s) 98
PsrI GAACNNNNNNTAC 2 cut(s) 381, 413
PstI CTGCAG 1 cut(s) 322
PsuI RGATCY 1 cut(s) 175
RsaI GTAC 4 cut(s) 105, 155, 390, 773
RsaNI GTAC 4 cut(s) 104, 154, 389, 772
SapI GCTCTTC 1 cut(s) 370
SaqAI TTAA 2 cut(s) 738, 949
SatI GCNGC 1 cut(s) 514
Sau3AI GATC 6 cut(s) 175, 493, 547, 613, 820, 924
Sau96I GGNCC 3 cut(s) 98, 112, 755
SchI GAGTC 1 cut(s) 72
ScrFI CCNGG 2 cut(s) 204, 874
SduI GDGCHC 1 cut(s) 338
SetI ASST 9 cut(s) 27, 103, 247, 252, 343, 456, 486, 696, 795
SfaNI GCATC 2 cut(s) 151, 200
SfcI CTRYAG 1 cut(s) 318
SinI GGWCC 1 cut(s) 98
SphI GCATGC 1 cut(s) 417
SsiI CCGC 3 cut(s) 386, 513, 649
SspI AATATT 2 cut(s) 279, 543
StyD4I CCNGG 2 cut(s) 202, 872
TaaI ACNGT 2 cut(s) 393, 570
TaqI TCGA 5 cut(s) 28, 61, 370, 768, 841
TatI WGTACW 3 cut(s) 103, 153, 771
TauI GCSGC 1 cut(s) 516
TfiI GAWTC 2 cut(s) 49, 944
Tru1I TTAA 2 cut(s) 738, 949
Tru9I TTAA 2 cut(s) 738, 949
TscAI CASTG 2 cut(s) 557, 575
TseFI GTSAC 1 cut(s) 564
Tsp45I GTSAC 1 cut(s) 564
TspDTI ATGAA 6 cut(s) 20, 70, 271, 286, 300, 681
TspRI CASTG 2 cut(s) 557, 575
VpaK11BI GGWCC 1 cut(s) 98
XapI RAATTY 3 cut(s) 232, 698, 712
XceI RCATGY 1 cut(s) 417
XmiI GTMKAC 1 cut(s) 746
XmnI GAANNNNTTC 2 cut(s) 53, 131
Zsp2I ATGCAT 2 cut(s) 193, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.