Rw4G029000

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001447
Physical Location & Seq
Reverse (-)
55701031 .. 55705761
4731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029000.1

Sequence Viewer

Length: 1242 bp
ATGAAGGGTGGTAGTGGTGGTTCTACTCAGAATCTGAGCACCGTAAATTTTTTTCCAAAGCTCATCAATGTCCAACTCCATCACCTCATCAGTGTCCTCTCTTATTTCCTCTTATTTGGTCTTGGAATAACCCTCGGGATGATCCTTACCTTCTATCTCAAAGACTTTTCTTTCAGTCTCCAACTGGCCCAATTCTCCTTCTCAACCTCATCATCTTCATCTAATCGAAACACGATACTGCCAAGTGTGACCGCTGCGCAAACCAATTTGAGTCACCCCCAGCACATCGGATTGGAGAAATTTCTGAAACCCCCTAAGATTTTGCACGACATGAATGATGAAGAATTGCTATGGAGAGCTTCTATGGCTCCACGGAGATCCGGATATCCATTCCATCGAGTTCCGAAAGTTGCCTTCTTGTTCTTGACAAAGGGGCCAGTTTTTCTGGCCCCATTGTGGGAGAAGTTCTTCAAAGGGCATCAAGGCTTGTACTCGATATATGTGCACTCGAATCCGTCTTACAATGGATCGTACCCCGAAAGTCCGGTGTTTCAAGGCAGAAGAATTCCTAGTCAGAGAGCAGAATGGGGGCAAGTGAACATGATTGAAGCCGAGAGCCGCGTAATTGCGAATGCTCTTCTCGACATCTCAAACCAACGTTTTGTTCTGCTTTCAGAATCATGCATCCCCCTCTTCAATTTCTCCACGGTCTACTCTTACCTCATAAACACCAACAAGACTTTTGTGCAGGTCTATGATGATCCAAGCGGTGTTGGGCGTGGCCGTTATGATGAAAGCTACTACCCTCAAATCACATTGGACCAGTGGAGGAAAGGATCGCAGTGGGTTGAAATAGATAGAGACCTTGCCATTGAAGTTGTTTCAGATCAAAAATACTACACAACTTTCCAGAAACTCTGCAAAGGCTATTGCTTTGCAGATGAGCATTACTTGCCAACATTTATGAACATAAGATTTCCGAAAAAGAATTCAAACAGGACTGTGACCTGGGTTGATTGGTCTAGGGGCGGACCTCATCCTGTCGAGTACGCGAGCACAGATGTCACGGTTGAGCTTCTGAGTACTCTGAGGAAGGGTAACGGCTGGTGTGATTACAATGGAAGGGGCACAGATGTTTGTTTCTTGTTCGCCCGGAAGTTCTCGCCAAATTCTTTGGATAAGCTGCTGAGCATTGCACCACAGTTAATGCAATTCAACACAGACATTGATAAAGAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

413

Amino Acids

47.21

Weight (kDa)

8.88

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 137 - 363 5.1e-75 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 258
Acc36I ACCTGC 1 cut(s) 739
AccI GTMKAC 1 cut(s) 711
AccII CGCG 2 cut(s) 621, 1052
AccIII TCCGGA 1 cut(s) 380
AciI CCGC 4 cut(s) 252, 619, 768, 1029
AclI AACGTT 1 cut(s) 658
AclWI GGATC 5 cut(s) 136, 372, 535, 755, 844
AcoI YGGCCR 1 cut(s) 781
AcsI RAATTY 5 cut(s) 46, 299, 564, 988, 1168
AfaI GTAC 5 cut(s) 491, 533, 1049, 1084, 1238
AfiI CCNNNNNNNGG 2 cut(s) 456, 457
AgsI TTSAA 8 cut(s) 472, 554, 608, 697, 851, 875, 993, 1216
AjnI CCWGG 1 cut(s) 1007
AjuI GAANNNNNNNTTGG 2 cut(s) 648, 680
AluBI AGCT 5 cut(s) 61, 359, 798, 1075, 1183
AluI AGCT 5 cut(s) 61, 359, 798, 1075, 1183
Alw21I GWGCWC 3 cut(s) 41, 507, 1058
Alw26I GTCTC 2 cut(s) 182, 855
Alw44I GTGCAC 1 cut(s) 503
AlwI GGATC 5 cut(s) 136, 372, 535, 755, 844
AlwNI CAGNNNCTG 1 cut(s) 34
Ama87I CYCGRG 1 cut(s) 134
Aor13HI TCCGGA 1 cut(s) 380
AoxI GGCC 4 cut(s) 186, 434, 447, 781
ApaLI GTGCAC 1 cut(s) 503
ApeKI GCWGC 2 cut(s) 254, 1183
ApoI RAATTY 5 cut(s) 46, 299, 564, 988, 1168
Asp700I GAANNNNTTC 1 cut(s) 467
AspLEI GCGC 1 cut(s) 259
AspS9I GGNCC 5 cut(s) 187, 434, 448, 820, 1031
AsuC2I CCSGG 1 cut(s) 1153
AsuHPI GGTGA 2 cut(s) 74, 266
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 2 cut(s) 820, 1031
BaeGI GKGCMC 2 cut(s) 507, 1130
Bbv12I GWGCWC 3 cut(s) 41, 507, 1058
BbvI GCAGC 2 cut(s) 241, 1170
BccI CCATC 2 cut(s) 87, 402
BceAI ACGGC 2 cut(s) 768, 1117
BcgI CGANNNNNNTGC 2 cut(s) 484, 518
BciT130I CCWGG 1 cut(s) 1009
BcnI CCSGG 1 cut(s) 1153
BcoDI GTCTC 2 cut(s) 182, 855
BfaI CTAG 3 cut(s) 570, 1023, 1240
BfuAI ACCTGC 1 cut(s) 739
BisI GCNGC 3 cut(s) 255, 619, 1184
BlpI GCTNAGC 1 cut(s) 1187
BlsI GCNGC 3 cut(s) 256, 620, 1185
BmcAI AGTACT 2 cut(s) 1084, 1238
Bme1390I CCNGG 2 cut(s) 1009, 1153
Bme18I GGWCC 2 cut(s) 820, 1031
BmeT110I CYCGRG 1 cut(s) 134
BmgT120I GGNCC 5 cut(s) 187, 434, 448, 820, 1031
BmiI GGNNCC 3 cut(s) 369, 435, 450
BmrFI CCNGG 2 cut(s) 1009, 1153
BmsI GCATC 2 cut(s) 487, 693
Bpu1102I GCTNAGC 1 cut(s) 1187
BpuMI CCSGG 1 cut(s) 1153
BsaI GGTCTC 1 cut(s) 855
BsaJI CCNNGG 4 cut(s) 133, 371, 705, 1008
BsaWI WCCGGW 2 cut(s) 380, 544
Bsc4I CCNNNNNNNGG 2 cut(s) 456, 457
Bse1I ACTGG 3 cut(s) 189, 437, 823
Bse3DI GCAATG 1 cut(s) 1191
BseAI TCCGGA 1 cut(s) 380
BseBI CCWGG 1 cut(s) 1009
BseDI CCNNGG 4 cut(s) 133, 371, 705, 1008
BseGI GGATG 3 cut(s) 144, 684, 1036
BseLI CCNNNNNNNGG 2 cut(s) 456, 457
BseMI GCAATG 1 cut(s) 1191
BseMII CTCAG 5 cut(s) 26, 41, 1070, 1079, 1178
BseNI ACTGG 3 cut(s) 189, 437, 823
BseSI GKGCMC 2 cut(s) 507, 1130
BseXI GCAGC 2 cut(s) 241, 1170
BseYI CCCAGC 1 cut(s) 279
BsgI GTGCAG 1 cut(s) 767
Bsh1236I CGCG 2 cut(s) 621, 1052
BshFI GGCC 4 cut(s) 188, 436, 449, 783
BsiHKAI GWGCWC 3 cut(s) 41, 507, 1058
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 3 cut(s) 381, 545, 1153
BslI CCNNNNNNNGG 2 cut(s) 456, 457
BsmAI GTCTC 2 cut(s) 182, 855
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 4 cut(s) 188, 436, 449, 783
Bso31I GGTCTC 1 cut(s) 855
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 4 cut(s) 41, 507, 1058, 1130
Bsp13I TCCGGA 1 cut(s) 380
Bsp143I GATC 6 cut(s) 141, 377, 527, 760, 836, 886
Bsp1720I GCTNAGC 1 cut(s) 1187
BspACI CCGC 4 cut(s) 252, 619, 768, 1029
BspANI GGCC 4 cut(s) 188, 436, 449, 783
BspCNI CTCAG 5 cut(s) 27, 40, 1071, 1080, 1179
BspEI TCCGGA 1 cut(s) 380
BspFNI CGCG 2 cut(s) 621, 1052
BspLI GGNNCC 3 cut(s) 369, 435, 450
BspMI ACCTGC 1 cut(s) 739
BspPI GGATC 5 cut(s) 136, 372, 535, 755, 844
BspQI GCTCTTC 1 cut(s) 642
BspTNI GGTCTC 1 cut(s) 855
BsrDI GCAATG 1 cut(s) 1191
BsrI ACTGG 3 cut(s) 189, 437, 823
BssECI CCNNGG 4 cut(s) 133, 371, 705, 1008
BssMI GATC 6 cut(s) 141, 377, 527, 760, 836, 886
Bst2UI CCWGG 1 cut(s) 1009
Bst4CI ACNGT 5 cut(s) 43, 709, 1003, 1069, 1203
Bst6I CTCTTC 2 cut(s) 642, 698
BstAPI GCANNNNNTGC 1 cut(s) 952
BstC8I GCNNGC 1 cut(s) 1054
BstDEI CTNAG 6 cut(s) 27, 35, 315, 1079, 1088, 1187
BstDSI CCRYGG 2 cut(s) 371, 705
BstF5I GGATG 3 cut(s) 144, 684, 1036
BstFNI CGCG 2 cut(s) 621, 1052
BstHHI GCGC 1 cut(s) 259
BstKTI GATC 6 cut(s) 144, 380, 530, 763, 839, 889
BstMAI GTCTC 2 cut(s) 182, 855
BstMBI GATC 6 cut(s) 141, 377, 527, 760, 836, 886
BstMWI GCNNNNNNNGC 2 cut(s) 365, 952
BstNI CCWGG 1 cut(s) 1009
BstSCI CCNGG 2 cut(s) 1007, 1151
BstSLI GKGCMC 2 cut(s) 507, 1130
BstUI CGCG 2 cut(s) 621, 1052
BstV1I GCAGC 2 cut(s) 241, 1170
BstX2I RGATCY 1 cut(s) 377
BstYI RGATCY 1 cut(s) 377
BsuRI GGCC 4 cut(s) 188, 436, 449, 783
BtgI CCRYGG 2 cut(s) 371, 705
BtsCI GGATG 3 cut(s) 144, 684, 1036
BtsI GCAGTG 1 cut(s) 848
BtsIMutI CAGTG 3 cut(s) 97, 830, 848
BveI ACCTGC 1 cut(s) 739
Cac8I GCNNGC 1 cut(s) 1054
CaiI CAGNNNCTG 1 cut(s) 34
CfoI GCGC 1 cut(s) 259
Cfr13I GGNCC 5 cut(s) 187, 434, 448, 820, 1031
Csp6I GTAC 5 cut(s) 490, 532, 1048, 1083, 1237
CviAII CATG 3 cut(s) 331, 601, 681
CviQI GTAC 5 cut(s) 490, 532, 1048, 1083, 1237
DdeI CTNAG 6 cut(s) 27, 35, 315, 1079, 1088, 1187
DpnI GATC 6 cut(s) 143, 379, 529, 762, 838, 888
DpnII GATC 6 cut(s) 141, 377, 527, 760, 836, 886
EaeI YGGCCR 1 cut(s) 781
Eam1104I CTCTTC 2 cut(s) 642, 698
EarI CTCTTC 2 cut(s) 642, 698
EciI GGCGGA 1 cut(s) 1044
Eco31I GGTCTC 1 cut(s) 855
Eco32I GATATC 1 cut(s) 386
Eco47I GGWCC 2 cut(s) 820, 1031
Eco88I CYCGRG 1 cut(s) 134
EcoRI GAATTC 2 cut(s) 564, 988
EcoRII CCWGG 1 cut(s) 1007
EcoRV GATATC 1 cut(s) 386
EcoT22I ATGCAT 1 cut(s) 686
FaeI CATG 3 cut(s) 334, 604, 684
FatI CATG 3 cut(s) 330, 600, 680
FblI GTMKAC 1 cut(s) 711
Fnu4HI GCNGC 3 cut(s) 255, 619, 1184
FokI GGATG 3 cut(s) 151, 671, 1023
Fsp4HI GCNGC 3 cut(s) 255, 619, 1184
FspBI CTAG 3 cut(s) 570, 1023, 1240
FspI TGCGCA 1 cut(s) 258
GlaI GCGC 1 cut(s) 258
GluI GCNGC 3 cut(s) 255, 619, 1184
GsaI CCCAGC 1 cut(s) 283
HaeIII GGCC 4 cut(s) 188, 436, 449, 783
HapII CCGG 3 cut(s) 381, 545, 1153
HhaI GCGC 1 cut(s) 259
Hin1II CATG 3 cut(s) 334, 604, 684
Hin6I GCGC 1 cut(s) 257
HinP1I GCGC 1 cut(s) 257
HinfI GANTC 4 cut(s) 31, 271, 511, 677
HpaII CCGG 3 cut(s) 381, 545, 1153
HphI GGTGA 2 cut(s) 74, 266
Hpy166II GTNNAC 3 cut(s) 505, 598, 712
Hpy188III TCNNGA 5 cut(s) 136, 381, 424, 641, 910
Hpy8I GTNNAC 3 cut(s) 505, 598, 712
HpyAV CCTTC 5 cut(s) 160, 208, 424, 1087, 1116
HpyCH4III ACNGT 5 cut(s) 43, 709, 1003, 1069, 1203
HpyCH4IV ACGT 1 cut(s) 658
HpyCH4V TGCA 8 cut(s) 325, 505, 684, 748, 921, 938, 1196, 1210
HpyF10VI GCNNNNNNNGC 2 cut(s) 365, 952
HpyF3I CTNAG 6 cut(s) 27, 35, 315, 1079, 1088, 1187
HpySE526I ACGT 1 cut(s) 658
Hsp92II CATG 3 cut(s) 334, 604, 684
HspAI GCGC 1 cut(s) 257
Kpn2I TCCGGA 1 cut(s) 380
Kzo9I GATC 6 cut(s) 141, 377, 527, 760, 836, 886
LguI GCTCTTC 1 cut(s) 642
LmnI GCTCC 1 cut(s) 373
Lsp1109I GCAGC 2 cut(s) 241, 1170
LweI GCATC 2 cut(s) 487, 693
MaeI CTAG 3 cut(s) 570, 1023, 1240
MaeII ACGT 1 cut(s) 658
MaeIII GTNAC 5 cut(s) 247, 272, 1003, 1063, 1097
MalI GATC 6 cut(s) 143, 379, 529, 762, 838, 888
MboI GATC 6 cut(s) 141, 377, 527, 760, 836, 886
MboII GAAGA 6 cut(s) 207, 353, 460, 573, 629, 685
MflI RGATCY 1 cut(s) 377
MhlI GDGCHC 4 cut(s) 41, 507, 1058, 1130
MlyI GAGTC 1 cut(s) 280
MmeI TCCRAC 2 cut(s) 97, 205
Mph1103I ATGCAT 1 cut(s) 686
MroI TCCGGA 1 cut(s) 380
MroXI GAANNNNTTC 1 cut(s) 467
MseI TTAA 1 cut(s) 1205
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 3 cut(s) 381, 545, 1153
MspR9I CCNGG 2 cut(s) 1009, 1153
Mva1269I GAATGC 1 cut(s) 637
MvaI CCWGG 1 cut(s) 1009
MvnI CGCG 2 cut(s) 621, 1052
MwoI GCNNNNNNNGC 2 cut(s) 365, 952
NciI CCSGG 1 cut(s) 1153
NdeII GATC 6 cut(s) 141, 377, 527, 760, 836, 886
NlaIII CATG 3 cut(s) 334, 604, 684
NlaIV GGNNCC 3 cut(s) 369, 435, 450
NmeAIII GCCGAG 1 cut(s) 637
NmuCI GTSAC 4 cut(s) 247, 272, 1003, 1063
NsbI TGCGCA 1 cut(s) 258
NsiI ATGCAT 1 cut(s) 686
PciSI GCTCTTC 1 cut(s) 642
PctI GAATGC 1 cut(s) 637
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 3 cut(s) 31, 511, 677
PkrI GCNGC 3 cut(s) 256, 620, 1185
PleI GAGTC 1 cut(s) 279
PpsI GAGTC 1 cut(s) 279
Psp1406I AACGTT 1 cut(s) 658
Psp6I CCWGG 1 cut(s) 1007
PspFI CCCAGC 1 cut(s) 279
PspGI CCWGG 1 cut(s) 1007
PspN4I GGNNCC 3 cut(s) 369, 435, 450
PspPI GGNCC 5 cut(s) 187, 434, 448, 820, 1031
PsrI GAACNNNNNNTAC 1 cut(s) 36
PstNI CAGNNNCTG 1 cut(s) 34
PsuI RGATCY 1 cut(s) 377
RsaI GTAC 5 cut(s) 491, 533, 1049, 1084, 1238
RsaNI GTAC 5 cut(s) 490, 532, 1048, 1083, 1237
SapI GCTCTTC 1 cut(s) 642
SaqAI TTAA 1 cut(s) 1205
SatI GCNGC 3 cut(s) 255, 619, 1184
Sau3AI GATC 6 cut(s) 141, 377, 527, 760, 836, 886
Sau96I GGNCC 5 cut(s) 187, 434, 448, 820, 1031
ScaI AGTACT 2 cut(s) 1084, 1238
SchI GAGTC 1 cut(s) 280
ScrFI CCNGG 2 cut(s) 1009, 1153
SduI GDGCHC 4 cut(s) 41, 507, 1058, 1130
SfaNI GCATC 2 cut(s) 487, 693
SinI GGWCC 2 cut(s) 820, 1031
SsiI CCGC 4 cut(s) 252, 619, 768, 1029
SspMI CTAG 3 cut(s) 570, 1023, 1240
StyD4I CCNGG 2 cut(s) 1007, 1151
TaaI ACNGT 5 cut(s) 43, 709, 1003, 1069, 1203
TaiI ACGT 1 cut(s) 661
TaqI TCGA 6 cut(s) 226, 397, 494, 509, 642, 1044
TatI WGTACW 3 cut(s) 489, 1082, 1236
TauI GCSGC 1 cut(s) 621
TfiI GAWTC 3 cut(s) 31, 511, 677
Tru1I TTAA 1 cut(s) 1205
Tru9I TTAA 1 cut(s) 1205
TscAI CASTG 3 cut(s) 97, 830, 848
TseFI GTSAC 4 cut(s) 247, 272, 1003, 1063
TseI GCWGC 2 cut(s) 254, 1183
Tsp45I GTSAC 4 cut(s) 247, 272, 1003, 1063
TspDTI ATGAA 6 cut(s) 17, 207, 347, 354, 807, 980
TspGWI ACGGA 2 cut(s) 388, 504
TspRI CASTG 3 cut(s) 97, 830, 848
VneI GTGCAC 1 cut(s) 503
VpaK11BI GGWCC 2 cut(s) 820, 1031
XapI RAATTY 5 cut(s) 46, 299, 564, 988, 1168
XmiI GTMKAC 1 cut(s) 711
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 3 cut(s) 570, 1023, 1240
ZrmI AGTACT 2 cut(s) 1084, 1238
Zsp2I ATGCAT 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.