MD13G1036900.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001447
Physical Location & Seq
Reverse (-)
2553728 .. 2554798
1071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1036900.v1.1.491

Sequence Viewer

Length: 981 bp
ATGAGCAAAATATGTGCAACCCAACCTAACATAACAGGAGGATATTTAAAGAAATATCTGAGGCCGCCAAAGGTGTTTCATGACATGAACGTCTCGGAATTGCTATGGAGAGCTTCGATGAGCCCTCGGATTCTCGAGTATCCAATCCATCTTGTTCCCAAGGTTGCTTTCATGTTCTTGACAAAGGGACCTGTTCATTTGGCCCTGTTATGGGAAAATTTCTTCAAAGGGCATCAAGGCTTGTACTCAATTTATGTGCACTCAAGTCCATCCTACGAGGCAGCATCGCATCCTGAAAGTCCGGTGTTTCATGAGCGAAGAATTCCTAGTCAGGAAGTGGAATGGGGGAAGGCGAGCTTGATTGAGGCTGTGCGCCGCCTATTGGCGAATGCTCTTCTCAACATCTCAAACCAACATTTTGTTCTCCTCTCAGAGACATGCATTCCCCTCTACAACTTTTCCACTGTCTACTCCTACCTCATCAACTCAACCCGAACTTTCGTAGAGGTCTTCGATGACCCAAGTTCGGTTGGACGTGGCCGGTACTATCTCAGCAACTACACGAGAATCACGGTAGATCAATGGAGAAAAGGGTGGGCGTGGTTTGCAATTGATAGAGAGGTTGCCATTGAATCCATCTCGGATCGAAAATACTTCCCGGAATTCCTCAAGTGCAAAGGCTATTGCTTTCCGGATGAACATTATTTGCCAACATTTGTGCACTTGAAATTTGCAGCAAGGAATGCAAATAGGACTTTGACCTGGGTTGACTGGAGCAAGGGCGGCCCACACCCGACGGAGTACAGGAGCCCAAACCTGACGGCGGCGCTTCTGAGTAGCCTGAGGAGTGGTTATGGTCGGAAATGTGAATACAATGGGAGGAGCACGGATGTTTGTTTCTTATTCGCCAGAAAGTTCATGCCTGATACTTTGGATAAACTGCTGAGACTTGCAACGCAGATCATGTATTTCAGCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

327

Amino Acids

37.79

Weight (kDa)

9.3

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 55 - 281 1.4e-72 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AccI GTMKAC 1 cut(s) 468
AccIII TCCGGA 1 cut(s) 691
AciI CCGC 4 cut(s) 65, 376, 783, 824
AclWI GGATC 1 cut(s) 651
AcoI YGGCCR 1 cut(s) 538
AcsI RAATTY 4 cut(s) 217, 321, 662, 728
AfaI GTAC 3 cut(s) 245, 545, 803
AfiI CCNNNNNNNGG 5 cut(s) 210, 211, 382, 526, 823
AgsI TTSAA 3 cut(s) 226, 632, 727
AjiI CACGTC 1 cut(s) 536
AjnI CCWGG 1 cut(s) 761
AjuI GAANNNNNNNTTGG 4 cut(s) 152, 184, 405, 437
AluBI AGCT 2 cut(s) 113, 357
AluI AGCT 2 cut(s) 113, 357
Alw21I GWGCWC 3 cut(s) 261, 723, 887
Alw26I GTCTC 3 cut(s) 97, 428, 940
Alw44I GTGCAC 2 cut(s) 257, 719
AlwI GGATC 1 cut(s) 651
Ama87I CYCGRG 1 cut(s) 134
Aor13HI TCCGGA 1 cut(s) 691
AoxI GGCC 4 cut(s) 62, 201, 538, 784
ApaLI GTGCAC 2 cut(s) 257, 719
ApeKI GCWGC 2 cut(s) 281, 734
ApoI RAATTY 4 cut(s) 217, 321, 662, 728
AspLEI GCGC 2 cut(s) 375, 829
AspS9I GGNCC 3 cut(s) 188, 202, 785
AsuC2I CCSGG 1 cut(s) 659
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 1 cut(s) 188
AxyI CCTNAGG 1 cut(s) 842
BaeGI GKGCMC 2 cut(s) 261, 723
BanII GRGCYC 2 cut(s) 125, 812
BauI CACGAG 1 cut(s) 562
BbsI GAAGAC 1 cut(s) 502
Bbv12I GWGCWC 3 cut(s) 261, 723, 887
BbvI GCAGC 2 cut(s) 293, 746
BccI CCATC 3 cut(s) 156, 277, 644
BceAI ACGGC 1 cut(s) 837
BciT130I CCWGG 1 cut(s) 763
BciVI GTATCC 1 cut(s) 150
BcnI CCSGG 1 cut(s) 659
BcoDI GTCTC 3 cut(s) 97, 428, 940
BfaI CTAG 1 cut(s) 327
BfoI RGCGCY 1 cut(s) 830
BfuI GTATCC 1 cut(s) 150
BisI GCNGC 6 cut(s) 65, 282, 376, 735, 784, 825
BlsI GCNGC 6 cut(s) 66, 283, 377, 736, 785, 826
Bme1390I CCNGG 2 cut(s) 659, 763
Bme18I GGWCC 1 cut(s) 188
BmeT110I CYCGRG 1 cut(s) 134
BmgBI CACGTC 1 cut(s) 536
BmgT120I GGNCC 3 cut(s) 188, 202, 785
BmiI GGNNCC 2 cut(s) 189, 809
BmrFI CCNGG 2 cut(s) 659, 763
BmsI GCATC 3 cut(s) 241, 293, 298
BpiI GAAGAC 1 cut(s) 502
BpmI CTGGAG 1 cut(s) 793
BpuEI CTTGAG 2 cut(s) 247, 653
BpuMI CCSGG 1 cut(s) 659
BsaJI CCNNGG 3 cut(s) 125, 159, 762
BsaWI WCCGGW 2 cut(s) 301, 691
Bsc4I CCNNNNNNNGG 5 cut(s) 210, 211, 382, 526, 823
Bse118I RCCGGY 1 cut(s) 540
Bse1I ACTGG 1 cut(s) 776
Bse21I CCTNAGG 1 cut(s) 842
BseAI TCCGGA 1 cut(s) 691
BseBI CCWGG 1 cut(s) 763
BseDI CCNNGG 3 cut(s) 125, 159, 762
BseGI GGATG 4 cut(s) 269, 289, 700, 895
BseLI CCNNNNNNNGG 5 cut(s) 210, 211, 382, 526, 823
BseMII CTCAG 6 cut(s) 50, 444, 565, 824, 833, 935
BseNI ACTGG 1 cut(s) 776
BseRI GAGGAG 3 cut(s) 416, 859, 895
BseSI GKGCMC 2 cut(s) 261, 723
BseXI GCAGC 2 cut(s) 293, 746
BshFI GGCC 4 cut(s) 64, 203, 540, 786
BsiHKAI GWGCWC 3 cut(s) 261, 723, 887
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 4 cut(s) 302, 541, 659, 692
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 5 cut(s) 210, 211, 382, 526, 823
BsmAI GTCTC 3 cut(s) 97, 428, 940
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 201
BsmI GAATGC 3 cut(s) 394, 441, 748
BsnI GGCC 4 cut(s) 64, 203, 540, 786
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 5 cut(s) 125, 261, 723, 812, 887
Bsp13I TCCGGA 1 cut(s) 691
Bsp143I GATC 3 cut(s) 577, 643, 960
BspACI CCGC 4 cut(s) 65, 376, 783, 824
BspANI GGCC 4 cut(s) 64, 203, 540, 786
BspCNI CTCAG 6 cut(s) 51, 443, 564, 825, 834, 936
BspEI TCCGGA 1 cut(s) 691
BspHI TCATGA 2 cut(s) 79, 310
BspLI GGNNCC 2 cut(s) 189, 809
BspPI GGATC 1 cut(s) 651
BspQI GCTCTTC 1 cut(s) 399
BsrFI RCCGGY 1 cut(s) 540
BsrI ACTGG 1 cut(s) 776
BssAI RCCGGY 1 cut(s) 540
BssECI CCNNGG 3 cut(s) 125, 159, 762
BssMI GATC 3 cut(s) 577, 643, 960
BssSI CACGAG 1 cut(s) 562
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 562
Bst2UI CCWGG 1 cut(s) 763
Bst4CI ACNGT 2 cut(s) 466, 574
Bst6I CTCTTC 1 cut(s) 399
BstAPI GCANNNNNTGC 1 cut(s) 743
BstC8I GCNNGC 1 cut(s) 355
BstDEI CTNAG 6 cut(s) 59, 430, 551, 833, 842, 944
BstF5I GGATG 4 cut(s) 269, 289, 700, 895
BstH2I RGCGCY 1 cut(s) 830
BstHHI GCGC 2 cut(s) 375, 829
BstKTI GATC 3 cut(s) 580, 646, 963
BstMAI GTCTC 3 cut(s) 97, 428, 940
BstMBI GATC 3 cut(s) 577, 643, 960
BstMWI GCNNNNNNNGC 3 cut(s) 605, 743, 783
BstNI CCWGG 1 cut(s) 763
BstNSI RCATGY 1 cut(s) 441
BstSCI CCNGG 2 cut(s) 657, 761
BstSLI GKGCMC 2 cut(s) 261, 723
BstV1I GCAGC 2 cut(s) 293, 746
BstV2I GAAGAC 1 cut(s) 502
Bsu36I CCTNAGG 1 cut(s) 842
BsuI GTATCC 1 cut(s) 150
BsuRI GGCC 4 cut(s) 64, 203, 540, 786
BtgZI GCGATG 1 cut(s) 270
BtrI CACGTC 1 cut(s) 536
BtsCI GGATG 4 cut(s) 269, 289, 700, 895
BtsIMutI CAGTG 1 cut(s) 462
Cac8I GCNNGC 1 cut(s) 355
CciI TCATGA 2 cut(s) 79, 310
CfoI GCGC 2 cut(s) 375, 829
Cfr10I RCCGGY 1 cut(s) 540
Cfr13I GGNCC 3 cut(s) 188, 202, 785
Csp6I GTAC 3 cut(s) 244, 544, 802
CviAII CATG 7 cut(s) 80, 85, 172, 311, 438, 919, 964
CviQI GTAC 3 cut(s) 244, 544, 802
DdeI CTNAG 6 cut(s) 59, 430, 551, 833, 842, 944
DpnI GATC 3 cut(s) 579, 645, 962
DpnII GATC 3 cut(s) 577, 643, 960
DraI TTTAAA 1 cut(s) 48
DrdI GACNNNNNNGTC 1 cut(s) 89
DseDI GACNNNNNNGTC 1 cut(s) 89
EaeI YGGCCR 1 cut(s) 538
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
Eco130I CCWWGG 1 cut(s) 159
Eco24I GRGCYC 2 cut(s) 125, 812
Eco47I GGWCC 1 cut(s) 188
Eco81I CCTNAGG 1 cut(s) 842
Eco88I CYCGRG 1 cut(s) 134
EcoO109I RGGNCCY 1 cut(s) 188
EcoRI GAATTC 2 cut(s) 321, 662
EcoRII CCWGG 1 cut(s) 761
EcoT14I CCWWGG 1 cut(s) 159
EcoT22I ATGCAT 1 cut(s) 443
EcoT38I GRGCYC 2 cut(s) 125, 812
ErhI CCWWGG 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 7 cut(s) 83, 88, 175, 314, 441, 922, 967
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 1 cut(s) 201
FatI CATG 7 cut(s) 79, 84, 171, 310, 437, 918, 963
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 6 cut(s) 65, 282, 376, 735, 784, 825
FokI GGATG 4 cut(s) 256, 276, 707, 902
FriOI GRGCYC 2 cut(s) 125, 812
Fsp4HI GCNGC 6 cut(s) 65, 282, 376, 735, 784, 825
FspBI CTAG 1 cut(s) 327
GlaI GCGC 2 cut(s) 374, 828
GluI GCNGC 6 cut(s) 65, 282, 376, 735, 784, 825
GsuI CTGGAG 1 cut(s) 793
HaeII RGCGCY 1 cut(s) 830
HaeIII GGCC 4 cut(s) 64, 203, 540, 786
HapII CCGG 4 cut(s) 302, 541, 659, 692
HhaI GCGC 2 cut(s) 375, 829
Hin1II CATG 7 cut(s) 83, 88, 175, 314, 441, 922, 967
Hin6I GCGC 2 cut(s) 373, 827
HinP1I GCGC 2 cut(s) 373, 827
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HinfI GANTC 3 cut(s) 130, 567, 632
HpaII CCGG 4 cut(s) 302, 541, 659, 692
Hpy166II GTNNAC 4 cut(s) 259, 469, 721, 769
Hpy188I TCNGA 7 cut(s) 60, 97, 129, 433, 643, 834, 861
Hpy188III TCNNGA 7 cut(s) 80, 134, 178, 293, 311, 332, 692
Hpy8I GTNNAC 4 cut(s) 259, 469, 721, 769
Hpy99I CGWCG 1 cut(s) 799
HpyAV CCTTC 1 cut(s) 343
HpyCH4III ACNGT 2 cut(s) 466, 574
HpyCH4IV ACGT 2 cut(s) 90, 535
HpyCH4V TGCA 9 cut(s) 17, 259, 441, 608, 675, 721, 734, 746, 953
HpyF10VI GCNNNNNNNGC 3 cut(s) 605, 743, 783
HpyF3I CTNAG 6 cut(s) 59, 430, 551, 833, 842, 944
HpySE526I ACGT 2 cut(s) 90, 535
Hsp92II CATG 7 cut(s) 83, 88, 175, 314, 441, 922, 967
HspAI GCGC 2 cut(s) 373, 827
Kpn2I TCCGGA 1 cut(s) 691
Kzo9I GATC 3 cut(s) 577, 643, 960
LguI GCTCTTC 1 cut(s) 399
LmnI GCTCC 3 cut(s) 774, 807, 882
Lsp1109I GCAGC 2 cut(s) 293, 746
LweI GCATC 3 cut(s) 241, 293, 298
MaeI CTAG 1 cut(s) 327
MaeII ACGT 2 cut(s) 90, 535
MalI GATC 3 cut(s) 579, 645, 962
MboI GATC 3 cut(s) 577, 643, 960
MboII GAAGA 4 cut(s) 214, 330, 386, 502
MfeI CAATTG 1 cut(s) 609
MhlI GDGCHC 5 cut(s) 125, 261, 723, 812, 887
MluCI AATT 7 cut(s) 98, 217, 249, 321, 609, 662, 728
MmeI TCCRAC 2 cut(s) 511, 839
Mph1103I ATGCAT 1 cut(s) 443
MroI TCCGGA 1 cut(s) 691
MseI TTAA 1 cut(s) 47
MspI CCGG 4 cut(s) 302, 541, 659, 692
MspR9I CCNGG 2 cut(s) 659, 763
MunI CAATTG 1 cut(s) 609
Mva1269I GAATGC 3 cut(s) 394, 441, 748
MvaI CCWGG 1 cut(s) 763
MwoI GCNNNNNNNGC 3 cut(s) 605, 743, 783
NciI CCSGG 1 cut(s) 659
NdeII GATC 3 cut(s) 577, 643, 960
NlaIII CATG 7 cut(s) 83, 88, 175, 314, 441, 922, 967
NlaIV GGNNCC 2 cut(s) 189, 809
NsiI ATGCAT 1 cut(s) 443
NspI RCATGY 1 cut(s) 441
PaeR7I CTCGAG 1 cut(s) 134
PagI TCATGA 2 cut(s) 79, 310
PciSI GCTCTTC 1 cut(s) 399
PctI GAATGC 3 cut(s) 394, 441, 748
PfeI GAWTC 3 cut(s) 130, 567, 632
PfoI TCCNGGA 1 cut(s) 657
PkrI GCNGC 6 cut(s) 66, 283, 377, 736, 785, 826
PpuMI RGGWCCY 1 cut(s) 188
Psp5II RGGWCCY 1 cut(s) 188
Psp6I CCWGG 1 cut(s) 761
PspGI CCWGG 1 cut(s) 761
PspN4I GGNNCC 2 cut(s) 189, 809
PspPI GGNCC 3 cut(s) 188, 202, 785
PspPPI RGGWCCY 1 cut(s) 188
RsaI GTAC 3 cut(s) 245, 545, 803
RsaNI GTAC 3 cut(s) 244, 544, 802
SapI GCTCTTC 1 cut(s) 399
SaqAI TTAA 1 cut(s) 47
SatI GCNGC 6 cut(s) 65, 282, 376, 735, 784, 825
Sau3AI GATC 3 cut(s) 577, 643, 960
Sau96I GGNCC 3 cut(s) 188, 202, 785
ScrFI CCNGG 2 cut(s) 659, 763
SduI GDGCHC 5 cut(s) 125, 261, 723, 812, 887
SfaNI GCATC 3 cut(s) 241, 293, 298
Sfr274I CTCGAG 1 cut(s) 134
SinI GGWCC 1 cut(s) 188
SlaI CTCGAG 1 cut(s) 134
SmlI CTYRAG 3 cut(s) 134, 262, 668
SmoI CTYRAG 3 cut(s) 134, 262, 668
Sse9I AATT 7 cut(s) 98, 217, 249, 321, 609, 662, 728
SsiI CCGC 4 cut(s) 65, 376, 783, 824
SspMI CTAG 1 cut(s) 327
StyD4I CCNGG 2 cut(s) 657, 761
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 2 cut(s) 466, 574
TaiI ACGT 2 cut(s) 93, 538
TaqI TCGA 4 cut(s) 116, 135, 513, 646
TasI AATT 7 cut(s) 98, 217, 249, 321, 609, 662, 728
TatI WGTACW 2 cut(s) 243, 801
TauI GCSGC 4 cut(s) 67, 378, 786, 827
TfiI GAWTC 3 cut(s) 130, 567, 632
Tru1I TTAA 1 cut(s) 47
Tru9I TTAA 1 cut(s) 47
TscAI CASTG 1 cut(s) 469
TseI GCWGC 2 cut(s) 281, 734
TspDTI ATGAA 7 cut(s) 68, 101, 160, 185, 299, 711, 907
TspGWI ACGGA 2 cut(s) 812, 902
TspRI CASTG 1 cut(s) 469
VneI GTGCAC 2 cut(s) 257, 719
VpaK11BI GGWCC 1 cut(s) 188
XapI RAATTY 4 cut(s) 217, 321, 662, 728
XceI RCATGY 1 cut(s) 441
XhoI CTCGAG 1 cut(s) 134
XmiI GTMKAC 1 cut(s) 468
XspI CTAG 1 cut(s) 327
Zsp2I ATGCAT 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.