Prupe.1G314700_v2.0.a1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001447
Physical Location & Seq
Forward (+)
30420382 .. 30422208
1827 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G314700.1

Sequence Viewer

Length: 1233 bp
ATGAAGGCTCAAGATCCAAACCCAGGAAGCGTTTTTCCCAAAGTCTTCAATGTCCAACGCCACCTCCTCAATCTCCTTTCTTATGTCCTGCTCTTTGTGGGCGGTTTAACTCTTGGAGTTGTACTTGTCAATCTCAAGGACTTTTCATTTGGTCTGCAATTTGCCCAATCCCCCTTCTCAACTTTGTCATCTCCTCCGCCATCCAATCATTCACAGATTCTAATTATGCCGAATGCAACAGCCCCAACAACCAGTATGAGCCTCACACCCGACCCCCGCATTGGGTTGAAGGAATATCTAAAGCCGCCGAAGGTTTTTCACGACATGAACGATACAGAATTGCTCTGGAGAGCTTCGATGTCTCCTAGGATCCCTGAATATCCATTCCATCTAGTTCCCAAAATTGCTTTCATGTTCTTGGCAAGGGGACCTGTTCTTTTGGCCCCATTGTGGGAGAAGTTCTTTGAAGGGCATCAAGGTTTTTACTCAATCTATGTGCACTCAGAGCCGTCCCACAATCAGTCGTCTTATGCCGGAAGTTCGGTTTTCCATGGCCGAAGAATTCCCAGTGATCTGAAAGTAAAATGGGGGACGGTGAGCTTGATTGAGGCAGAGCGCCTCCTATTGGCCAATGCTCTTCTCGACATCTCAAACCAACGCTTTGTTCTGCTCTCAGAAGCATGCATCCCTCTCTACAACTTCTCCACTGTCTACTCTTACCTCATAAACTCCAAAGAAACTTTCGTGGAGGTCTATGATGATCCAAGCGCAGTTGGACGTGGCCGATATTATTTTGTTCAGTACCCAGGAATCTCAGTGGAGCAATGGAGGAAGGGGTCACAGTGGTTTGAAATTGACAGAGACCTTGCCATTGAAGTTGTCTCGGATCGAAAATACTTCCCAGTTTACCGCAGATGCAGAGGCGAATGTTTTGCAGATGAACATTATTTGCCAACGTTTGTGAACATCAAATTTGGCGCAAAGAATGCAAACAGGACGTTGACTTGGGTTGACTGGGCCAAGGGCGGCCCGCACCCTACCGAGTACATGAGCTTGAATGTCACAGTCGAGCTTTTGAATGGCCTGAGGACTGGTTATGGCCGGAGATGTGAATATAATGGAAGGAGCACACATGTTTGTTTCTTATTCGCGAGGAAGTTCCCGCCTTCTGCTTTGGACAGCCTGCTGAGAATTGCACCAAAGATCATGCACTTCAACAATGTCGCTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

411

Amino Acids

46.65

Weight (kDa)

9.11

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 711
AccII CGCG 1 cut(s) 1151
AciI CCGC 8 cut(s) 102, 197, 277, 305, 910, 1026, 1031, 1163
AclI AACGTT 1 cut(s) 956
AclWI GGATC 5 cut(s) 8, 364, 377, 755, 894
AcoI YGGCCR 4 cut(s) 553, 627, 781, 1099
AcsI RAATTY 2 cut(s) 561, 971
AfaI GTAC 3 cut(s) 123, 803, 1046
AfiI CCNNNNNNNGG 6 cut(s) 23, 281, 282, 450, 451, 625
AflIII ACRYGT 1 cut(s) 1132
AgsI TTSAA 8 cut(s) 49, 289, 467, 851, 875, 1057, 1078, 1216
AjiI CACGTC 1 cut(s) 779
AjnI CCWGG 2 cut(s) 22, 805
AjuI GAANNNNNNNTTGG 6 cut(s) 392, 424, 648, 680, 725, 757
AluBI AGCT 4 cut(s) 353, 600, 1053, 1072
AluI AGCT 4 cut(s) 353, 600, 1053, 1072
Alw21I GWGCWC 2 cut(s) 501, 1130
Alw26I GTCTC 3 cut(s) 366, 855, 886
Alw44I GTGCAC 1 cut(s) 497
AlwI GGATC 5 cut(s) 8, 364, 377, 755, 894
AoxI GGCC 8 cut(s) 441, 553, 627, 781, 1017, 1027, 1081, 1099
ApaLI GTGCAC 1 cut(s) 497
ApoI RAATTY 2 cut(s) 561, 971
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AspA2I CCTAGG 1 cut(s) 365
AspLEI GCGC 3 cut(s) 618, 770, 980
AspS9I GGNCC 4 cut(s) 428, 442, 1017, 1028
AsuHPI GGTGA 1 cut(s) 607
AvaII GGWCC 1 cut(s) 428
AvrII CCTAGG 1 cut(s) 365
AxyI CCTNAGG 1 cut(s) 1085
BaeGI GKGCMC 1 cut(s) 501
BalI TGGCCA 1 cut(s) 629
BamHI GGATCC 1 cut(s) 369
BbsI GAAGAC 1 cut(s) 37
Bbv12I GWGCWC 2 cut(s) 501, 1130
BccI CCATC 2 cut(s) 208, 396
BceAI ACGGC 1 cut(s) 493
BcgI CGANNNNNNTGC 2 cut(s) 914, 948
BciT130I CCWGG 2 cut(s) 24, 807
BcoDI GTCTC 3 cut(s) 366, 855, 886
BfaI CTAG 2 cut(s) 366, 392
BfoI RGCGCY 1 cut(s) 619
BisI GCNGC 2 cut(s) 305, 1027
BlnI CCTAGG 1 cut(s) 365
BlsI GCNGC 2 cut(s) 306, 1028
Bme1390I CCNGG 2 cut(s) 24, 807
Bme18I GGWCC 1 cut(s) 428
BmgBI CACGTC 1 cut(s) 779
BmgT120I GGNCC 4 cut(s) 428, 442, 1017, 1028
BmiI GGNNCC 3 cut(s) 371, 429, 444
BmrFI CCNGG 2 cut(s) 24, 807
BmrI ACTGGG 3 cut(s) 561, 896, 1024
BmsI GCATC 3 cut(s) 481, 693, 905
BmuI ACTGGG 3 cut(s) 561, 896, 1024
BpiI GAAGAC 1 cut(s) 37
BpmI CTGGAG 1 cut(s) 367
BpuEI CTTGAG 1 cut(s) 119
BsaI GGTCTC 1 cut(s) 855
BsaJI CCNNGG 5 cut(s) 22, 365, 550, 805, 1020
BsaXI ACNNNNNCTCC 4 cut(s) 48, 78, 686, 716
Bsc4I CCNNNNNNNGG 6 cut(s) 23, 281, 282, 450, 451, 625
Bse1I ACTGG 5 cut(s) 252, 567, 902, 1019, 1096
Bse21I CCTNAGG 1 cut(s) 1085
Bse3DI GCAATG 1 cut(s) 830
BseBI CCWGG 2 cut(s) 24, 807
BseDI CCNNGG 5 cut(s) 22, 365, 550, 805, 1020
BseGI GGATG 2 cut(s) 200, 684
BseLI CCNNNNNNNGG 6 cut(s) 23, 281, 282, 450, 451, 625
BseMI GCAATG 1 cut(s) 830
BseMII CTCAG 5 cut(s) 516, 687, 828, 1076, 1178
BseNI ACTGG 5 cut(s) 252, 567, 902, 1019, 1096
BseRI GAGGAG 2 cut(s) 56, 183
BseSI GKGCMC 1 cut(s) 501
Bsh1236I CGCG 1 cut(s) 1151
BshFI GGCC 8 cut(s) 443, 555, 629, 783, 1019, 1029, 1083, 1101
BsiHKAI GWGCWC 2 cut(s) 501, 1130
BsiSI CCGG 2 cut(s) 534, 1102
BslFI GGGAC 3 cut(s) 441, 496, 604
BslI CCNNNNNNNGG 6 cut(s) 23, 281, 282, 450, 451, 625
BsmAI GTCTC 3 cut(s) 366, 855, 886
BsmFI GGGAC 3 cut(s) 441, 496, 604
BsmI GAATGC 2 cut(s) 238, 991
BsnI GGCC 8 cut(s) 443, 555, 629, 783, 1019, 1029, 1083, 1101
Bso31I GGTCTC 1 cut(s) 855
Bsp1286I GDGCHC 2 cut(s) 501, 1130
Bsp143I GATC 6 cut(s) 13, 369, 571, 760, 886, 1203
Bsp19I CCATGG 1 cut(s) 550
Bsp68I TCGCGA 1 cut(s) 1151
BspACI CCGC 8 cut(s) 102, 197, 277, 305, 910, 1026, 1031, 1163
BspANI GGCC 8 cut(s) 443, 555, 629, 783, 1019, 1029, 1083, 1101
BspCNI CTCAG 5 cut(s) 515, 686, 827, 1077, 1179
BspFNI CGCG 1 cut(s) 1151
BspLI GGNNCC 3 cut(s) 371, 429, 444
BspPI GGATC 5 cut(s) 8, 364, 377, 755, 894
BspQI GCTCTTC 1 cut(s) 642
BspTNI GGTCTC 1 cut(s) 855
BsrDI GCAATG 1 cut(s) 830
BsrI ACTGG 5 cut(s) 252, 567, 902, 1019, 1096
BssECI CCNNGG 5 cut(s) 22, 365, 550, 805, 1020
BssMI GATC 6 cut(s) 13, 369, 571, 760, 886, 1203
BssT1I CCWWGG 3 cut(s) 365, 550, 1020
Bst2UI CCWGG 2 cut(s) 24, 807
Bst4CI ACNGT 4 cut(s) 595, 709, 843, 1066
Bst6I CTCTTC 1 cut(s) 642
BstAPI GCANNNNNTGC 1 cut(s) 986
BstC8I GCNNGC 3 cut(s) 682, 1031, 1184
BstDEI CTNAG 5 cut(s) 502, 673, 814, 1085, 1187
BstDSI CCRYGG 1 cut(s) 550
BstF5I GGATG 2 cut(s) 200, 684
BstFNI CGCG 1 cut(s) 1151
BstH2I RGCGCY 1 cut(s) 619
BstHHI GCGC 3 cut(s) 618, 770, 980
BstKTI GATC 6 cut(s) 16, 372, 574, 763, 889, 1206
BstMAI GTCTC 3 cut(s) 366, 855, 886
BstMBI GATC 6 cut(s) 13, 369, 571, 760, 886, 1203
BstMWI GCNNNNNNNGC 2 cut(s) 505, 986
BstNI CCWGG 2 cut(s) 24, 807
BstNSI RCATGY 2 cut(s) 684, 1136
BstSCI CCNGG 2 cut(s) 22, 805
BstSLI GKGCMC 1 cut(s) 501
BstUI CGCG 1 cut(s) 1151
BstV2I GAAGAC 1 cut(s) 37
BstX2I RGATCY 2 cut(s) 13, 369
BstYI RGATCY 2 cut(s) 13, 369
Bsu36I CCTNAGG 1 cut(s) 1085
BsuRI GGCC 8 cut(s) 443, 555, 629, 783, 1019, 1029, 1083, 1101
BtgI CCRYGG 1 cut(s) 550
BtrI CACGTC 1 cut(s) 779
BtsCI GGATG 2 cut(s) 200, 684
BtsIMutI CAGTG 4 cut(s) 574, 705, 822, 848
BtuMI TCGCGA 1 cut(s) 1151
Cac8I GCNNGC 3 cut(s) 682, 1031, 1184
CfoI GCGC 3 cut(s) 618, 770, 980
Cfr13I GGNCC 4 cut(s) 428, 442, 1017, 1028
Csp6I GTAC 3 cut(s) 122, 802, 1045
CviAII CATG 7 cut(s) 325, 412, 551, 681, 1048, 1133, 1207
CviQI GTAC 3 cut(s) 122, 802, 1045
DdeI CTNAG 5 cut(s) 502, 673, 814, 1085, 1187
DpnI GATC 6 cut(s) 15, 371, 573, 762, 888, 1205
DpnII GATC 6 cut(s) 13, 369, 571, 760, 886, 1203
EaeI YGGCCR 4 cut(s) 553, 627, 781, 1099
Eam1104I CTCTTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 642
EciI GGCGGA 1 cut(s) 186
Eco130I CCWWGG 3 cut(s) 365, 550, 1020
Eco31I GGTCTC 1 cut(s) 855
Eco47I GGWCC 1 cut(s) 428
Eco81I CCTNAGG 1 cut(s) 1085
EcoO109I RGGNCCY 1 cut(s) 428
EcoRI GAATTC 1 cut(s) 561
EcoRII CCWGG 2 cut(s) 22, 805
EcoT14I CCWWGG 3 cut(s) 365, 550, 1020
EcoT22I ATGCAT 1 cut(s) 686
ErhI CCWWGG 3 cut(s) 365, 550, 1020
FaeI CATG 7 cut(s) 328, 415, 554, 684, 1051, 1136, 1210
FaqI GGGAC 3 cut(s) 441, 496, 604
FatI CATG 7 cut(s) 324, 411, 550, 680, 1047, 1132, 1206
FauI CCCGC 3 cut(s) 284, 1038, 1170
FblI GTMKAC 1 cut(s) 711
Fnu4HI GCNGC 2 cut(s) 305, 1027
FokI GGATG 2 cut(s) 187, 671
Fsp4HI GCNGC 2 cut(s) 305, 1027
FspBI CTAG 2 cut(s) 366, 392
GlaI GCGC 3 cut(s) 617, 769, 979
GluI GCNGC 2 cut(s) 305, 1027
GsuI CTGGAG 1 cut(s) 367
HaeII RGCGCY 1 cut(s) 619
HaeIII GGCC 8 cut(s) 443, 555, 629, 783, 1019, 1029, 1083, 1101
HapII CCGG 2 cut(s) 534, 1102
HhaI GCGC 3 cut(s) 618, 770, 980
Hin1II CATG 7 cut(s) 328, 415, 554, 684, 1051, 1136, 1210
Hin6I GCGC 3 cut(s) 616, 768, 978
HinP1I GCGC 3 cut(s) 616, 768, 978
HincII GTYRAC 2 cut(s) 1002, 1012
HindII GTYRAC 2 cut(s) 1002, 1012
HinfI GANTC 2 cut(s) 217, 810
HpaII CCGG 2 cut(s) 534, 1102
HphI GGTGA 1 cut(s) 607
Hpy166II GTNNAC 6 cut(s) 499, 712, 907, 964, 1002, 1012
Hpy188I TCNGA 4 cut(s) 505, 576, 676, 886
Hpy188III TCNNGA 5 cut(s) 11, 320, 346, 641, 1150
Hpy8I GTNNAC 6 cut(s) 499, 712, 907, 964, 1002, 1012
HpyAV CCTTC 7 cut(s) 184, 283, 304, 461, 826, 1116, 1176
HpyCH4III ACNGT 4 cut(s) 595, 709, 843, 1066
HpyCH4IV ACGT 3 cut(s) 778, 956, 998
HpyCH4V TGCA 9 cut(s) 157, 236, 499, 684, 918, 935, 989, 1196, 1210
HpyF10VI GCNNNNNNNGC 2 cut(s) 505, 986
HpyF3I CTNAG 5 cut(s) 502, 673, 814, 1085, 1187
HpySE526I ACGT 3 cut(s) 778, 956, 998
Hsp92II CATG 7 cut(s) 328, 415, 554, 684, 1051, 1136, 1210
HspAI GCGC 3 cut(s) 616, 768, 978
Kzo9I GATC 6 cut(s) 13, 369, 571, 760, 886, 1203
LguI GCTCTTC 1 cut(s) 642
LmnI GCTCC 3 cut(s) 820, 1125, 1231
LweI GCATC 3 cut(s) 481, 693, 905
MaeI CTAG 2 cut(s) 366, 392
MaeII ACGT 3 cut(s) 778, 956, 998
MaeIII GTNAC 2 cut(s) 837, 1060
MalI GATC 6 cut(s) 15, 371, 573, 762, 888, 1205
MboI GATC 6 cut(s) 13, 369, 571, 760, 886, 1203
MboII GAAGA 3 cut(s) 37, 570, 629
MflI RGATCY 2 cut(s) 13, 369
MhlI GDGCHC 2 cut(s) 501, 1130
MlsI TGGCCA 1 cut(s) 629
MluCI AATT 8 cut(s) 158, 222, 338, 402, 561, 852, 971, 1191
MluNI TGGCCA 1 cut(s) 629
MmeI TCCRAC 2 cut(s) 79, 754
Mox20I TGGCCA 1 cut(s) 629
Mph1103I ATGCAT 1 cut(s) 686
MscI TGGCCA 1 cut(s) 629
MseI TTAA 2 cut(s) 107, 1231
Msp20I TGGCCA 1 cut(s) 629
MspI CCGG 2 cut(s) 534, 1102
MspR9I CCNGG 2 cut(s) 24, 807
Mva1269I GAATGC 2 cut(s) 238, 991
MvaI CCWGG 2 cut(s) 24, 807
MvnI CGCG 1 cut(s) 1151
MwoI GCNNNNNNNGC 2 cut(s) 505, 986
NcoI CCATGG 1 cut(s) 550
NdeII GATC 6 cut(s) 13, 369, 571, 760, 886, 1203
NlaIII CATG 7 cut(s) 328, 415, 554, 684, 1051, 1136, 1210
NlaIV GGNNCC 3 cut(s) 371, 429, 444
NmuCI GTSAC 2 cut(s) 837, 1060
NruI TCGCGA 1 cut(s) 1151
NsiI ATGCAT 1 cut(s) 686
NspI RCATGY 2 cut(s) 684, 1136
PaeI GCATGC 1 cut(s) 684
PciI ACATGT 1 cut(s) 1132
PciSI GCTCTTC 1 cut(s) 642
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PctI GAATGC 2 cut(s) 238, 991
PfeI GAWTC 2 cut(s) 217, 810
PkrI GCNGC 2 cut(s) 306, 1028
PpuMI RGGWCCY 1 cut(s) 428
PscI ACATGT 1 cut(s) 1132
Psp1406I AACGTT 1 cut(s) 956
Psp5II RGGWCCY 1 cut(s) 428
Psp6I CCWGG 2 cut(s) 22, 805
PspGI CCWGG 2 cut(s) 22, 805
PspN4I GGNNCC 3 cut(s) 371, 429, 444
PspPI GGNCC 4 cut(s) 428, 442, 1017, 1028
PspPPI RGGWCCY 1 cut(s) 428
PsuI RGATCY 2 cut(s) 13, 369
RruI TCGCGA 1 cut(s) 1151
RsaI GTAC 3 cut(s) 123, 803, 1046
RsaNI GTAC 3 cut(s) 122, 802, 1045
SapI GCTCTTC 1 cut(s) 642
SaqAI TTAA 2 cut(s) 107, 1231
SatI GCNGC 2 cut(s) 305, 1027
Sau3AI GATC 6 cut(s) 13, 369, 571, 760, 886, 1203
Sau96I GGNCC 4 cut(s) 428, 442, 1017, 1028
ScrFI CCNGG 2 cut(s) 24, 807
SduI GDGCHC 2 cut(s) 501, 1130
SfaNI GCATC 3 cut(s) 481, 693, 905
SinI GGWCC 1 cut(s) 428
SmlI CTYRAG 2 cut(s) 9, 134
SmoI CTYRAG 2 cut(s) 9, 134
SphI GCATGC 1 cut(s) 684
Sse9I AATT 8 cut(s) 158, 222, 338, 402, 561, 852, 971, 1191
SsiI CCGC 8 cut(s) 102, 197, 277, 305, 910, 1026, 1031, 1163
SspMI CTAG 2 cut(s) 366, 392
StyD4I CCNGG 2 cut(s) 22, 805
StyI CCWWGG 3 cut(s) 365, 550, 1020
TaaI ACNGT 4 cut(s) 595, 709, 843, 1066
TaiI ACGT 3 cut(s) 781, 959, 1001
TaqI TCGA 4 cut(s) 356, 642, 889, 1068
TasI AATT 8 cut(s) 158, 222, 338, 402, 561, 852, 971, 1191
TatI WGTACW 2 cut(s) 121, 1044
TauI GCSGC 2 cut(s) 307, 1029
TfiI GAWTC 2 cut(s) 217, 810
Tru1I TTAA 2 cut(s) 107, 1231
Tru9I TTAA 2 cut(s) 107, 1231
TscAI CASTG 4 cut(s) 574, 712, 822, 848
TseFI GTSAC 2 cut(s) 837, 1060
Tsp45I GTSAC 2 cut(s) 837, 1060
TspDTI ATGAA 5 cut(s) 17, 135, 341, 400, 954
TspRI CASTG 4 cut(s) 574, 712, 822, 848
VneI GTGCAC 1 cut(s) 497
VpaK11BI GGWCC 1 cut(s) 428
XapI RAATTY 2 cut(s) 561, 971
XceI RCATGY 2 cut(s) 684, 1136
XmaJI CCTAGG 1 cut(s) 365
XmiI GTMKAC 1 cut(s) 711
XspI CTAG 2 cut(s) 366, 392
Zsp2I ATGCAT 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.