Prupe.1G314600_v2.0.a1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001447
Physical Location & Seq
Forward (+)
30417237 .. 30419925
2689 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G314600.2

Sequence Viewer

Length: 1197 bp
ATGAAGGGTCAGAATCAAAACCCATTAAACTTGTTTTCAAAACTCTTCAATGCCCAACTCCACATCCTCAAACTCCTCTTCTATTTCCTGCTATTTGGTGGTGGTTTAACCCTTGGAATGGTACTTAGCTTTTATCTGAAAGACATTTCAATTAGTTTGCAATTAGCCCAATTCTCCTTCTCAACCTCACAATCTCCATCCAATCAACATACCCTAATGCCAAATGTAAAAGCCTTCGAAGCCAATGCGACCAAGCCTCACATCGGATTGAAGGAATATCTAAAGCCGCCTAATGTTCTGCACGACATGGACGACCAAGAATTGCTATGGAGAGCTTCGATGATTCCTCGGATTCCTGAGTATCCATTCCGTCGAGTTCCCAAGGTTGCTTTCATGTTCTTGACAAAGGGTCCTGTTCTGCTGGCCCCACTGTGGGAGAAGTTCTTCAAAGGGCATCGGGGCTTGTACTCAATTTATGTGCACTCAAATCCATCTTACAATGGATCAGACCCCGAAAGTCCTGTTTTCCATGACAGGAGAATTCCTAGCCGGGAAGTAGAATGGGGAAATGTGAACATGATTGAGGCTGAGCGCCGCCTATTGGCAAATGCTCTTCTTGACATCTCAAACCAACGTTTTGTTCTGCTCTCAGAATCCTGCATCCCCCTCCACAACTTCTCCACTGTCTACTCTTACCTCGTACGCTCCCGCGAAAGTTTTGTACAGGTCTATGATGATCCAAGCTCTGTCGGACGGGGCCGGTATAATTCCCATATGTACCCAAAAATCTCATTGAGCCAATGGAGGAAAGGATCACAGTGGTTTGAGATGGACAGACGCCATGCCATTGAAATTGTCTCAGATAAAAAATACTTCCCAGCTTTCCAAAAGTACTGCAGGGGCTCATGCTATGCAGACGAGCATTATCTGCCAACATTTGCGAACATCAACTTTGGGGCAAAGAATTCGAATAGGAGTTTGACCTGGGTTGACTGGTCCAGGGGTGGCCCGCACCCTGCCAAGTTCACAAGAACTGATGTCACCGTTGAGCTTTTGAGTAGATTGAGGAATGGGACTGCGTGTGTATATAATGGAAAGAGCACCAACGTTTGTTTCTTGTTTGCTAGGAAGTTTGGTCCTGATACTTTGGAAAGGCTGCTGAGGTTTGCTCCAAAGCTCATGCGCTTCAACAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

399

Amino Acids

46.08

Weight (kDa)

9.73

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 687
AccII CGCG 1 cut(s) 711
AciI CCGC 4 cut(s) 287, 595, 709, 1010
AclI AACGTT 2 cut(s) 634, 1107
AclWI GGATC 3 cut(s) 511, 731, 820
AcsI RAATTY 2 cut(s) 540, 964
AcyI GRCGYC 1 cut(s) 838
AfaI GTAC 6 cut(s) 123, 467, 702, 723, 779, 893
AfiI CCNNNNNNNGG 5 cut(s) 118, 263, 432, 433, 601
AgsI TTSAA 7 cut(s) 39, 49, 150, 271, 448, 851, 1189
AhdI GACNNNNNGTC 1 cut(s) 408
AjnI CCWGG 2 cut(s) 983, 998
AjuI GAANNNNNNNTTGG 6 cut(s) 374, 406, 624, 656, 1097, 1129
AluBI AGCT 6 cut(s) 129, 335, 744, 881, 1051, 1177
AluI AGCT 6 cut(s) 129, 335, 744, 881, 1051, 1177
Alw21I GWGCWC 2 cut(s) 483, 1103
Alw26I GTCTC 1 cut(s) 862
Alw44I GTGCAC 1 cut(s) 479
AlwI GGATC 3 cut(s) 511, 731, 820
AoxI GGCC 3 cut(s) 423, 757, 1006
ApaLI GTGCAC 1 cut(s) 479
ApeKI GCWGC 1 cut(s) 1156
ApoI RAATTY 2 cut(s) 540, 964
Asp700I GAANNNNTTC 1 cut(s) 443
AspLEI GCGC 2 cut(s) 594, 1185
AspS9I GGNCC 6 cut(s) 410, 424, 757, 996, 1007, 1136
AsuC2I CCSGG 1 cut(s) 551
AsuHPI GGTGA 1 cut(s) 1033
AsuII TTCGAA 2 cut(s) 237, 968
AvaII GGWCC 3 cut(s) 410, 996, 1136
BaeGI GKGCMC 1 cut(s) 483
BanII GRGCYC 1 cut(s) 905
Bbv12I GWGCWC 2 cut(s) 483, 1103
BbvCI CCTCAGC 1 cut(s) 1160
BbvI GCAGC 1 cut(s) 1143
BccI CCATC 3 cut(s) 205, 499, 823
BcgI CGANNNNNNTGC 4 cut(s) 227, 261, 948, 982
BciT130I CCWGG 2 cut(s) 985, 1000
BciVI GTATCC 1 cut(s) 372
BcnI CCSGG 1 cut(s) 551
BcoDI GTCTC 1 cut(s) 862
BfaI CTAG 2 cut(s) 546, 1125
BfmI CTRYAG 1 cut(s) 895
BfoI RGCGCY 1 cut(s) 595
BfuI GTATCC 1 cut(s) 372
BisI GCNGC 3 cut(s) 287, 595, 1157
BlpI GCTNAGC 1 cut(s) 588
BlsI GCNGC 3 cut(s) 288, 596, 1158
BmcAI AGTACT 1 cut(s) 893
Bme1390I CCNGG 3 cut(s) 551, 985, 1000
Bme18I GGWCC 3 cut(s) 410, 996, 1136
BmeRI GACNNNNNGTC 1 cut(s) 408
BmgT120I GGNCC 6 cut(s) 410, 424, 757, 996, 1007, 1136
BmiI GGNNCC 3 cut(s) 411, 426, 758
BmrFI CCNGG 3 cut(s) 551, 985, 1000
BmsI GCATC 2 cut(s) 463, 669
BplI GAGNNNNNCTC 2 cut(s) 1153, 1185
Bpu10I CCTNAGC 1 cut(s) 1160
Bpu1102I GCTNAGC 1 cut(s) 588
Bpu14I TTCGAA 2 cut(s) 237, 968
BpuMI CCSGG 1 cut(s) 551
BsaHI GRCGYC 1 cut(s) 838
BsaJI CCNNGG 5 cut(s) 112, 347, 381, 984, 999
BsaXI ACNNNNNCTCC 2 cut(s) 662, 692
Bsc4I CCNNNNNNNGG 5 cut(s) 118, 263, 432, 433, 601
Bse118I RCCGGY 1 cut(s) 759
Bse1I ACTGG 1 cut(s) 998
BseBI CCWGG 2 cut(s) 985, 1000
BseDI CCNNGG 5 cut(s) 112, 347, 381, 984, 999
BseGI GGATG 3 cut(s) 63, 197, 660
BseLI CCNNNNNNNGG 5 cut(s) 118, 263, 432, 433, 601
BseMII CTCAG 5 cut(s) 348, 579, 663, 873, 1151
BseNI ACTGG 1 cut(s) 998
BseRI GAGGAG 1 cut(s) 65
BseSI GKGCMC 1 cut(s) 483
BseXI GCAGC 1 cut(s) 1143
BseYI CCCAGC 1 cut(s) 877
BsgI GTGCAG 1 cut(s) 284
Bsh1236I CGCG 1 cut(s) 711
BshFI GGCC 3 cut(s) 425, 759, 1008
BsiHKAI GWGCWC 2 cut(s) 483, 1103
BsiSI CCGG 2 cut(s) 550, 760
BsiWI CGTACG 1 cut(s) 700
BslFI GGGAC 1 cut(s) 1087
BslI CCNNNNNNNGG 5 cut(s) 118, 263, 432, 433, 601
BsmAI GTCTC 1 cut(s) 862
BsmFI GGGAC 1 cut(s) 1087
BsnI GGCC 3 cut(s) 425, 759, 1008
Bsp119I TTCGAA 2 cut(s) 237, 968
Bsp1286I GDGCHC 3 cut(s) 483, 905, 1103
Bsp1407I TGTACA 1 cut(s) 721
Bsp143I GATC 3 cut(s) 503, 736, 812
Bsp1720I GCTNAGC 1 cut(s) 588
BspACI CCGC 4 cut(s) 287, 595, 709, 1010
BspANI GGCC 3 cut(s) 425, 759, 1008
BspCNI CTCAG 5 cut(s) 349, 580, 662, 872, 1152
BspFNI CGCG 1 cut(s) 711
BspLI GGNNCC 3 cut(s) 411, 426, 758
BspMAI CTGCAG 1 cut(s) 899
BspPI GGATC 3 cut(s) 511, 731, 820
BspQI GCTCTTC 1 cut(s) 618
BspT104I TTCGAA 2 cut(s) 237, 968
BsrFI RCCGGY 1 cut(s) 759
BsrGI TGTACA 1 cut(s) 721
BsrI ACTGG 1 cut(s) 998
BssAI RCCGGY 1 cut(s) 759
BssECI CCNNGG 5 cut(s) 112, 347, 381, 984, 999
BssMI GATC 3 cut(s) 503, 736, 812
BssNI GRCGYC 1 cut(s) 838
BssT1I CCWWGG 2 cut(s) 112, 381
Bst2UI CCWGG 2 cut(s) 985, 1000
Bst4CI ACNGT 4 cut(s) 432, 685, 819, 1045
Bst6I CTCTTC 3 cut(s) 50, 83, 618
BstACI GRCGYC 1 cut(s) 838
BstAPI GCANNNNNTGC 1 cut(s) 928
BstAUI TGTACA 1 cut(s) 721
BstBI TTCGAA 2 cut(s) 237, 968
BstC8I GCNNGC 2 cut(s) 423, 1010
BstDEI CTNAG 6 cut(s) 125, 357, 588, 649, 859, 1160
BstF5I GGATG 3 cut(s) 63, 197, 660
BstFNI CGCG 1 cut(s) 711
BstH2I RGCGCY 1 cut(s) 595
BstHHI GCGC 2 cut(s) 594, 1185
BstKTI GATC 3 cut(s) 506, 739, 815
BstMAI GTCTC 1 cut(s) 862
BstMBI GATC 3 cut(s) 503, 736, 812
BstMWI GCNNNNNNNGC 2 cut(s) 239, 928
BstNI CCWGG 2 cut(s) 985, 1000
BstSCI CCNGG 3 cut(s) 549, 983, 998
BstSFI CTRYAG 1 cut(s) 895
BstSLI GKGCMC 1 cut(s) 483
BstUI CGCG 1 cut(s) 711
BstV1I GCAGC 1 cut(s) 1143
BsuI GTATCC 1 cut(s) 372
BsuRI GGCC 3 cut(s) 425, 759, 1008
BtsCI GGATG 3 cut(s) 63, 197, 660
BtsIMutI CAGTG 3 cut(s) 428, 681, 824
Cac8I GCNNGC 2 cut(s) 423, 1010
CfoI GCGC 2 cut(s) 594, 1185
Cfr10I RCCGGY 1 cut(s) 759
Cfr13I GGNCC 6 cut(s) 410, 424, 757, 996, 1007, 1136
CseI GACGC 1 cut(s) 846
Csp6I GTAC 6 cut(s) 122, 466, 701, 722, 778, 892
CviAII CATG 7 cut(s) 307, 394, 530, 577, 842, 906, 1180
CviQI GTAC 6 cut(s) 122, 466, 701, 722, 778, 892
DdeI CTNAG 6 cut(s) 125, 357, 588, 649, 859, 1160
DpnI GATC 3 cut(s) 505, 738, 814
DpnII GATC 3 cut(s) 503, 736, 812
DriI GACNNNNNGTC 1 cut(s) 408
Eam1104I CTCTTC 3 cut(s) 50, 83, 618
Eam1105I GACNNNNNGTC 1 cut(s) 408
EarI CTCTTC 3 cut(s) 50, 83, 618
Eco130I CCWWGG 2 cut(s) 112, 381
Eco24I GRGCYC 1 cut(s) 905
Eco47I GGWCC 3 cut(s) 410, 996, 1136
EcoO109I RGGNCCY 1 cut(s) 410
EcoRI GAATTC 2 cut(s) 540, 964
EcoRII CCWGG 2 cut(s) 983, 998
EcoT14I CCWWGG 2 cut(s) 112, 381
EcoT38I GRGCYC 1 cut(s) 905
ErhI CCWWGG 2 cut(s) 112, 381
FaeI CATG 7 cut(s) 310, 397, 533, 580, 845, 909, 1183
FaqI GGGAC 1 cut(s) 1087
FatI CATG 7 cut(s) 306, 393, 529, 576, 841, 905, 1179
FauI CCCGC 2 cut(s) 716, 1017
FauNDI CATATG 1 cut(s) 774
FblI GTMKAC 1 cut(s) 687
Fnu4HI GCNGC 3 cut(s) 287, 595, 1157
FokI GGATG 3 cut(s) 50, 184, 647
FriOI GRGCYC 1 cut(s) 905
Fsp4HI GCNGC 3 cut(s) 287, 595, 1157
FspBI CTAG 2 cut(s) 546, 1125
GlaI GCGC 2 cut(s) 593, 1184
GluI GCNGC 3 cut(s) 287, 595, 1157
GsaI CCCAGC 1 cut(s) 881
HaeII RGCGCY 1 cut(s) 595
HaeIII GGCC 3 cut(s) 425, 759, 1008
HapII CCGG 2 cut(s) 550, 760
HgaI GACGC 1 cut(s) 846
HhaI GCGC 2 cut(s) 594, 1185
Hin1I GRCGYC 1 cut(s) 838
Hin1II CATG 7 cut(s) 310, 397, 533, 580, 845, 909, 1183
Hin6I GCGC 2 cut(s) 592, 1183
HinP1I GCGC 2 cut(s) 592, 1183
HincII GTYRAC 1 cut(s) 991
HindII GTYRAC 1 cut(s) 991
HinfI GANTC 4 cut(s) 13, 343, 352, 653
HpaII CCGG 2 cut(s) 550, 760
HphI GGTGA 1 cut(s) 1033
Hpy166II GTNNAC 5 cut(s) 481, 574, 688, 991, 1026
Hpy188I TCNGA 8 cut(s) 12, 138, 266, 351, 508, 652, 752, 862
Hpy188III TCNNGA 4 cut(s) 356, 400, 617, 1139
Hpy8I GTNNAC 5 cut(s) 481, 574, 688, 991, 1026
Hpy99I CGWCG 1 cut(s) 375
HpyAV CCTTC 3 cut(s) 187, 244, 265
HpyCH4III ACNGT 4 cut(s) 432, 685, 819, 1045
HpyCH4IV ACGT 2 cut(s) 634, 1107
HpyCH4V TGCA 6 cut(s) 160, 301, 481, 660, 897, 914
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 928
HpyF3I CTNAG 6 cut(s) 125, 357, 588, 649, 859, 1160
HpySE526I ACGT 2 cut(s) 634, 1107
Hsp92I GRCGYC 1 cut(s) 838
Hsp92II CATG 7 cut(s) 310, 397, 533, 580, 845, 909, 1183
HspAI GCGC 2 cut(s) 592, 1183
Kzo9I GATC 3 cut(s) 503, 736, 812
LguI GCTCTTC 1 cut(s) 618
LmnI GCTCC 2 cut(s) 710, 1174
Lsp1109I GCAGC 1 cut(s) 1143
LweI GCATC 2 cut(s) 463, 669
MaeI CTAG 2 cut(s) 546, 1125
MaeII ACGT 2 cut(s) 634, 1107
MaeIII GTNAC 1 cut(s) 1039
MalI GATC 3 cut(s) 505, 738, 814
MboI GATC 3 cut(s) 503, 736, 812
MboII GAAGA 4 cut(s) 37, 70, 436, 605
MhlI GDGCHC 3 cut(s) 483, 905, 1103
MluCI AATT 9 cut(s) 150, 161, 170, 320, 471, 540, 766, 852, 964
MmeI TCCRAC 1 cut(s) 730
MroXI GAANNNNTTC 1 cut(s) 443
MseI TTAA 2 cut(s) 26, 107
MspI CCGG 2 cut(s) 550, 760
MspR9I CCNGG 3 cut(s) 551, 985, 1000
MvaI CCWGG 2 cut(s) 985, 1000
MvnI CGCG 1 cut(s) 711
MwoI GCNNNNNNNGC 2 cut(s) 239, 928
NciI CCSGG 1 cut(s) 551
NdeI CATATG 1 cut(s) 774
NdeII GATC 3 cut(s) 503, 736, 812
NlaIII CATG 7 cut(s) 310, 397, 533, 580, 845, 909, 1183
NlaIV GGNNCC 3 cut(s) 411, 426, 758
NmuCI GTSAC 1 cut(s) 1039
NspV TTCGAA 2 cut(s) 237, 968
PciSI GCTCTTC 1 cut(s) 618
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 4 cut(s) 13, 343, 352, 653
Pfl23II CGTACG 1 cut(s) 700
PkrI GCNGC 3 cut(s) 288, 596, 1158
PpuMI RGGWCCY 1 cut(s) 410
Psp1406I AACGTT 2 cut(s) 634, 1107
Psp5II RGGWCCY 1 cut(s) 410
Psp6I CCWGG 2 cut(s) 983, 998
PspFI CCCAGC 1 cut(s) 877
PspGI CCWGG 2 cut(s) 983, 998
PspLI CGTACG 1 cut(s) 700
PspN4I GGNNCC 3 cut(s) 411, 426, 758
PspPI GGNCC 6 cut(s) 410, 424, 757, 996, 1007, 1136
PspPPI RGGWCCY 1 cut(s) 410
PstI CTGCAG 1 cut(s) 899
RsaI GTAC 6 cut(s) 123, 467, 702, 723, 779, 893
RsaNI GTAC 6 cut(s) 122, 466, 701, 722, 778, 892
SapI GCTCTTC 1 cut(s) 618
SaqAI TTAA 2 cut(s) 26, 107
SatI GCNGC 3 cut(s) 287, 595, 1157
Sau3AI GATC 3 cut(s) 503, 736, 812
Sau96I GGNCC 6 cut(s) 410, 424, 757, 996, 1007, 1136
ScaI AGTACT 1 cut(s) 893
ScrFI CCNGG 3 cut(s) 551, 985, 1000
SduI GDGCHC 3 cut(s) 483, 905, 1103
SfaNI GCATC 2 cut(s) 463, 669
SfcI CTRYAG 1 cut(s) 895
SfuI TTCGAA 2 cut(s) 237, 968
SinI GGWCC 3 cut(s) 410, 996, 1136
Sse9I AATT 9 cut(s) 150, 161, 170, 320, 471, 540, 766, 852, 964
SsiI CCGC 4 cut(s) 287, 595, 709, 1010
SspMI CTAG 2 cut(s) 546, 1125
StyD4I CCNGG 3 cut(s) 549, 983, 998
StyI CCWWGG 2 cut(s) 112, 381
TaaI ACNGT 4 cut(s) 432, 685, 819, 1045
TaiI ACGT 2 cut(s) 637, 1110
TaqI TCGA 4 cut(s) 237, 338, 373, 968
TasI AATT 9 cut(s) 150, 161, 170, 320, 471, 540, 766, 852, 964
TatI WGTACW 3 cut(s) 465, 721, 891
TauI GCSGC 2 cut(s) 289, 597
TfiI GAWTC 4 cut(s) 13, 343, 352, 653
Tru1I TTAA 2 cut(s) 26, 107
Tru9I TTAA 2 cut(s) 26, 107
TscAI CASTG 3 cut(s) 435, 688, 824
TseFI GTSAC 1 cut(s) 1039
TseI GCWGC 1 cut(s) 1156
Tsp45I GTSAC 1 cut(s) 1039
TspDTI ATGAA 2 cut(s) 17, 382
TspGWI ACGGA 1 cut(s) 359
TspRI CASTG 3 cut(s) 435, 688, 824
VneI GTGCAC 1 cut(s) 479
VpaK11BI GGWCC 3 cut(s) 410, 996, 1136
XapI RAATTY 2 cut(s) 540, 964
XmiI GTMKAC 1 cut(s) 687
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 2 cut(s) 546, 1125
ZrmI AGTACT 1 cut(s) 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.