RchiOBHm_Chr4g0434741

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0001447
Physical Location & Seq
Reverse (-)
58418986 .. 58420751
1766 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40319

Sequence Viewer

Length: 1251 bp
ATGAAGGATCATAATCAAAACCCTTCAAACTTCTTCACAAAACTAATCAATGTCCAAGGCCACCTCCTCAATCTCCTCTCTTATTTCCTCACCTTTGGTGGTGGTTTAGTTCTCGGAATCGTACTTGCCTCTTCTTATCTCAAAGACTCTTCCTTTAGTCTACAAATAGCCCAACTATCATTCTCCTCTTCTTCTTCTTCATCATCTCCGTCCAATGGTTCCAATATTTCACATATGACAATTAGTCCAAATGACACAGTCACGATGTCTAACTTGACCGAAGTTCATATACGAGAATTAGAGGCATTTCTAAAGCCGCCTAAGGTTCGGCACGACATGGACGATGAGGAATTGTTCTGGAGAGCTTCCATGGCCCCTCGGATACCCGAATATCCGTTCCACCGAGTTCCGAAAGTTGCTTTCATGTTCTTGACAAGGGGACCTGTTCATTTGGCTCCATTGTGGGAGAAGTTCTTCCAAGGGCACCAAGGCTTGTTCTCAATCTATGTCCACTCAGATCGGTCTTATAATGAATCGTCTCACCCCGAAAGCCCAGTCTTTCATGGCCGAAGAATTCCAAGTCAGAGAGCAGAATGGGGGCAAGTGAACATGATTGAAGCCGAGAGCCGCCTAATTGCGAATGCTCTTCTCGACATCTCAAACCAACGTTTTGTTCTGCTTTCAGAATCATGCATCCCCCTCTTCAATTTCTCCACAGTCTACTCTTACCTCATAAACACCAACAAGACTTTTGTGCAGGTCTATGATGATCCAAGCGGTGTTGGGCGTGGCCGTTATGATGAAAGCTACTACCCTCAAATCACATTGGACCAGTGGAGGAAAGGATCGCAGTGGGTTGAAATAGATAGAGACCTTGCCATTGAAGTTGTTTCAGATCAAAAATACTACACAACTTTCCAGAAACTCTGCAAAGGCTATTGCTTTGCAGATGAGCATTACTTGCCAACATTTATGAACATAAGATTTCCGAAAAAGAATTCAAACAGGACTGTGACCTGGGTTGATTGGTCTAGGGGCGGACCTCATCCTGTCGAGTACGCGAGCACAGATGTCACGGTTGAGCTTCTGAGTACTCTGAGGAAGGGTAACGGCTGGTGTGATTACAATGGAAGGGGCACAGATGTTTGTTTCTTGTTCGCCCGGAAGTTCTCGCCAAATTCTTTGGATAAGCTGCTGAGCATTGCACCACAGATAATGCAATTCAACACAGACATTGATAAAGAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

416

Amino Acids

47.68

Weight (kDa)

6.5

Isoelectric Point (pI)

40.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 139 - 366 3.4e-77 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 528
Acc36I ACCTGC 1 cut(s) 748
AccB1I GGYRCC 1 cut(s) 483
AccI GTMKAC 2 cut(s) 160, 720
AccII CGCG 1 cut(s) 1061
AciI CCGC 4 cut(s) 317, 628, 777, 1038
AclI AACGTT 1 cut(s) 667
AclWI GGATC 3 cut(s) 15, 764, 853
AcoI YGGCCR 2 cut(s) 565, 790
AcsI RAATTY 3 cut(s) 573, 997, 1177
AfaI GTAC 4 cut(s) 123, 1058, 1093, 1247
AfiI CCNNNNNNNGG 1 cut(s) 215
AgsI TTSAA 7 cut(s) 27, 617, 706, 860, 884, 1002, 1225
AhdI GACNNNNNGTC 1 cut(s) 243
AjnI CCWGG 1 cut(s) 1016
AjuI GAANNNNNNNTTGG 2 cut(s) 657, 689
AluBI AGCT 4 cut(s) 365, 807, 1084, 1192
AluI AGCT 4 cut(s) 365, 807, 1084, 1192
Alw21I GWGCWC 1 cut(s) 1067
Alw26I GTCTC 2 cut(s) 543, 864
AlwI GGATC 3 cut(s) 15, 764, 853
AoxI GGCC 4 cut(s) 58, 372, 565, 790
ApeKI GCWGC 1 cut(s) 1192
ApoI RAATTY 3 cut(s) 573, 997, 1177
ArsI GACNNNNNNTTYG 2 cut(s) 540, 572
Asp700I GAANNNNTTC 1 cut(s) 473
AspS9I GGNCC 4 cut(s) 373, 440, 829, 1040
AsuC2I CCSGG 1 cut(s) 1162
AsuHPI GGTGA 2 cut(s) 82, 533
AvaII GGWCC 3 cut(s) 440, 829, 1040
AxyI CCTNAGG 1 cut(s) 321
BaeGI GKGCMC 2 cut(s) 486, 1139
BanI GGYRCC 1 cut(s) 483
BarI GAAGNNNNNNTAC 2 cut(s) 273, 305
Bbv12I GWGCWC 1 cut(s) 1067
BbvI GCAGC 1 cut(s) 1179
BceAI ACGGC 2 cut(s) 777, 1126
BciT130I CCWGG 1 cut(s) 1018
BciVI GTATCC 1 cut(s) 375
BcnI CCSGG 1 cut(s) 1162
BcoDI GTCTC 2 cut(s) 543, 864
BfaI CTAG 2 cut(s) 1032, 1249
BfuAI ACCTGC 1 cut(s) 748
BfuI GTATCC 1 cut(s) 375
BisI GCNGC 3 cut(s) 317, 628, 1193
BlpI GCTNAGC 1 cut(s) 1196
BlsI GCNGC 3 cut(s) 318, 629, 1194
BmcAI AGTACT 2 cut(s) 1093, 1247
Bme1390I CCNGG 2 cut(s) 1018, 1162
Bme18I GGWCC 3 cut(s) 440, 829, 1040
BmeRI GACNNNNNGTC 1 cut(s) 243
BmgT120I GGNCC 4 cut(s) 373, 440, 829, 1040
BmiI GGNNCC 5 cut(s) 220, 375, 441, 456, 485
BmrFI CCNGG 2 cut(s) 1018, 1162
BmrI ACTGGG 1 cut(s) 548
BmsI GCATC 1 cut(s) 702
BmuI ACTGGG 1 cut(s) 548
BpmI CTGGAG 1 cut(s) 379
Bpu1102I GCTNAGC 1 cut(s) 1196
BpuMI CCSGG 1 cut(s) 1162
BsaBI GATNNNNATC 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 864
BsaJI CCNNGG 6 cut(s) 55, 369, 377, 478, 487, 1017
Bsc4I CCNNNNNNNGG 1 cut(s) 215
Bse1I ACTGG 2 cut(s) 554, 832
Bse21I CCTNAGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 1200
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 1 cut(s) 1018
BseDI CCNNGG 6 cut(s) 55, 369, 377, 478, 487, 1017
BseGI GGATG 2 cut(s) 693, 1045
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 1 cut(s) 215
BseMI GCAATG 1 cut(s) 1200
BseMII CTCAG 4 cut(s) 528, 1079, 1088, 1187
BseNI ACTGG 2 cut(s) 554, 832
BseRI GAGGAG 3 cut(s) 56, 65, 175
BseSI GKGCMC 2 cut(s) 486, 1139
BseXI GCAGC 1 cut(s) 1179
BsgI GTGCAG 1 cut(s) 776
Bsh1236I CGCG 1 cut(s) 1061
BshFI GGCC 4 cut(s) 60, 374, 567, 792
BshNI GGYRCC 1 cut(s) 483
BsiHKAI GWGCWC 1 cut(s) 1067
BsiSI CCGG 1 cut(s) 1162
BslFI GGGAC 1 cut(s) 453
BslI CCNNNNNNNGG 1 cut(s) 215
BsmAI GTCTC 2 cut(s) 543, 864
BsmBI CGTCTC 1 cut(s) 543
BsmFI GGGAC 1 cut(s) 453
BsmI GAATGC 1 cut(s) 646
BsnI GGCC 4 cut(s) 60, 374, 567, 792
Bso31I GGTCTC 1 cut(s) 864
Bsp1286I GDGCHC 3 cut(s) 486, 1067, 1139
Bsp143I GATC 5 cut(s) 7, 517, 769, 845, 895
Bsp1720I GCTNAGC 1 cut(s) 1196
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 4 cut(s) 317, 628, 777, 1038
BspANI GGCC 4 cut(s) 60, 374, 567, 792
BspCNI CTCAG 4 cut(s) 527, 1080, 1089, 1188
BspFNI CGCG 1 cut(s) 1061
BspLI GGNNCC 5 cut(s) 220, 375, 441, 456, 485
BspMI ACCTGC 1 cut(s) 748
BspPI GGATC 3 cut(s) 15, 764, 853
BspQI GCTCTTC 1 cut(s) 651
BspT107I GGYRCC 1 cut(s) 483
BspTNI GGTCTC 1 cut(s) 864
BsrDI GCAATG 1 cut(s) 1200
BsrI ACTGG 2 cut(s) 554, 832
BssECI CCNNGG 6 cut(s) 55, 369, 377, 478, 487, 1017
BssMI GATC 5 cut(s) 7, 517, 769, 845, 895
BssT1I CCWWGG 4 cut(s) 55, 369, 478, 487
Bst2UI CCWGG 1 cut(s) 1018
Bst4CI ACNGT 4 cut(s) 259, 718, 1012, 1078
Bst6I CTCTTC 5 cut(s) 136, 154, 193, 651, 707
BstAPI GCANNNNNTGC 1 cut(s) 961
BstC8I GCNNGC 1 cut(s) 1063
BstDEI CTNAG 5 cut(s) 321, 514, 1088, 1097, 1196
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 2 cut(s) 693, 1045
BstFNI CGCG 1 cut(s) 1061
BstKTI GATC 5 cut(s) 10, 520, 772, 848, 898
BstMAI GTCTC 2 cut(s) 543, 864
BstMBI GATC 5 cut(s) 7, 517, 769, 845, 895
BstMWI GCNNNNNNNGC 2 cut(s) 371, 961
BstNI CCWGG 1 cut(s) 1018
BstSCI CCNGG 2 cut(s) 1016, 1160
BstSLI GKGCMC 2 cut(s) 486, 1139
BstUI CGCG 1 cut(s) 1061
BstV1I GCAGC 1 cut(s) 1179
Bsu36I CCTNAGG 1 cut(s) 321
BsuI GTATCC 1 cut(s) 375
BsuRI GGCC 4 cut(s) 60, 374, 567, 792
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 2 cut(s) 693, 1045
BtsI GCAGTG 1 cut(s) 857
BtsIMutI CAGTG 2 cut(s) 839, 857
BveI ACCTGC 1 cut(s) 748
Cac8I GCNNGC 1 cut(s) 1063
Cfr13I GGNCC 4 cut(s) 373, 440, 829, 1040
Csp6I GTAC 4 cut(s) 122, 1057, 1092, 1246
CviAII CATG 6 cut(s) 337, 370, 424, 563, 610, 690
CviQI GTAC 4 cut(s) 122, 1057, 1092, 1246
DdeI CTNAG 5 cut(s) 321, 514, 1088, 1097, 1196
DpnI GATC 5 cut(s) 9, 519, 771, 847, 897
DpnII GATC 5 cut(s) 7, 517, 769, 845, 895
DriI GACNNNNNGTC 1 cut(s) 243
EaeI YGGCCR 2 cut(s) 565, 790
Eam1104I CTCTTC 5 cut(s) 136, 154, 193, 651, 707
Eam1105I GACNNNNNGTC 1 cut(s) 243
EarI CTCTTC 5 cut(s) 136, 154, 193, 651, 707
EciI GGCGGA 1 cut(s) 1053
Eco130I CCWWGG 4 cut(s) 55, 369, 478, 487
Eco31I GGTCTC 1 cut(s) 864
Eco47I GGWCC 3 cut(s) 440, 829, 1040
Eco81I CCTNAGG 1 cut(s) 321
EcoO109I RGGNCCY 1 cut(s) 440
EcoRI GAATTC 2 cut(s) 573, 997
EcoRII CCWGG 1 cut(s) 1016
EcoT14I CCWWGG 4 cut(s) 55, 369, 478, 487
EcoT22I ATGCAT 1 cut(s) 695
ErhI CCWWGG 4 cut(s) 55, 369, 478, 487
Esp3I CGTCTC 1 cut(s) 543
FaeI CATG 6 cut(s) 340, 373, 427, 566, 613, 693
FaqI GGGAC 1 cut(s) 453
FatI CATG 6 cut(s) 336, 369, 423, 562, 609, 689
FauNDI CATATG 1 cut(s) 234
FblI GTMKAC 2 cut(s) 160, 720
Fnu4HI GCNGC 3 cut(s) 317, 628, 1193
FokI GGATG 2 cut(s) 680, 1032
Fsp4HI GCNGC 3 cut(s) 317, 628, 1193
FspBI CTAG 2 cut(s) 1032, 1249
GluI GCNGC 3 cut(s) 317, 628, 1193
GsuI CTGGAG 1 cut(s) 379
HaeIII GGCC 4 cut(s) 60, 374, 567, 792
HapII CCGG 1 cut(s) 1162
Hin1II CATG 6 cut(s) 340, 373, 427, 566, 613, 693
HinfI GANTC 4 cut(s) 117, 146, 533, 686
HpaII CCGG 1 cut(s) 1162
HphI GGTGA 2 cut(s) 82, 533
Hpy166II GTNNAC 4 cut(s) 161, 511, 607, 721
Hpy188III TCNNGA 5 cut(s) 262, 358, 430, 650, 919
Hpy8I GTNNAC 4 cut(s) 161, 511, 607, 721
HpyAV CCTTC 3 cut(s) 33, 1096, 1125
HpyCH4III ACNGT 4 cut(s) 259, 718, 1012, 1078
HpyCH4IV ACGT 1 cut(s) 667
HpyCH4V TGCA 6 cut(s) 693, 757, 930, 947, 1205, 1219
HpyF10VI GCNNNNNNNGC 2 cut(s) 371, 961
HpyF3I CTNAG 5 cut(s) 321, 514, 1088, 1097, 1196
HpySE526I ACGT 1 cut(s) 667
Hsp92II CATG 6 cut(s) 340, 373, 427, 566, 613, 693
Kzo9I GATC 5 cut(s) 7, 517, 769, 845, 895
LguI GCTCTTC 1 cut(s) 651
LmnI GCTCC 1 cut(s) 460
Lsp1109I GCAGC 1 cut(s) 1179
LweI GCATC 1 cut(s) 702
MaeI CTAG 2 cut(s) 1032, 1249
MaeII ACGT 1 cut(s) 667
MaeIII GTNAC 4 cut(s) 259, 1012, 1072, 1106
MalI GATC 5 cut(s) 9, 519, 771, 847, 897
MboI GATC 5 cut(s) 7, 517, 769, 845, 895
MhlI GDGCHC 3 cut(s) 486, 1067, 1139
MluCI AATT 9 cut(s) 240, 296, 350, 573, 633, 706, 997, 1177, 1220
MlyI GAGTC 1 cut(s) 140
Mph1103I ATGCAT 1 cut(s) 695
MroXI GAANNNNTTC 1 cut(s) 473
MspI CCGG 1 cut(s) 1162
MspR9I CCNGG 2 cut(s) 1018, 1162
Mva1269I GAATGC 1 cut(s) 646
MvaI CCWGG 1 cut(s) 1018
MvnI CGCG 1 cut(s) 1061
MwoI GCNNNNNNNGC 2 cut(s) 371, 961
NciI CCSGG 1 cut(s) 1162
NcoI CCATGG 1 cut(s) 369
NdeI CATATG 1 cut(s) 234
NdeII GATC 5 cut(s) 7, 517, 769, 845, 895
NlaIII CATG 6 cut(s) 340, 373, 427, 566, 613, 693
NlaIV GGNNCC 5 cut(s) 220, 375, 441, 456, 485
NmeAIII GCCGAG 1 cut(s) 646
NmuCI GTSAC 3 cut(s) 259, 1012, 1072
NsiI ATGCAT 1 cut(s) 695
PciSI GCTCTTC 1 cut(s) 651
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 646
PdmI GAANNNNTTC 1 cut(s) 473
PfeI GAWTC 3 cut(s) 117, 533, 686
PflFI GACNNNGTC 1 cut(s) 257
PkrI GCNGC 3 cut(s) 318, 629, 1194
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PpuMI RGGWCCY 1 cut(s) 440
PsiI TTATAA 1 cut(s) 528
Psp1406I AACGTT 1 cut(s) 667
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 1016
PspGI CCWGG 1 cut(s) 1016
PspN4I GGNNCC 5 cut(s) 220, 375, 441, 456, 485
PspPI GGNCC 4 cut(s) 373, 440, 829, 1040
PspPPI RGGWCCY 1 cut(s) 440
PsyI GACNNNGTC 1 cut(s) 257
RsaI GTAC 4 cut(s) 123, 1058, 1093, 1247
RsaNI GTAC 4 cut(s) 122, 1057, 1092, 1246
SapI GCTCTTC 1 cut(s) 651
SatI GCNGC 3 cut(s) 317, 628, 1193
Sau3AI GATC 5 cut(s) 7, 517, 769, 845, 895
Sau96I GGNCC 4 cut(s) 373, 440, 829, 1040
ScaI AGTACT 2 cut(s) 1093, 1247
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 1018, 1162
SduI GDGCHC 3 cut(s) 486, 1067, 1139
SfaNI GCATC 1 cut(s) 702
SinI GGWCC 3 cut(s) 440, 829, 1040
Sse9I AATT 9 cut(s) 240, 296, 350, 573, 633, 706, 997, 1177, 1220
SsiI CCGC 4 cut(s) 317, 628, 777, 1038
SspI AATATT 1 cut(s) 226
SspMI CTAG 2 cut(s) 1032, 1249
StyD4I CCNGG 2 cut(s) 1016, 1160
StyI CCWWGG 4 cut(s) 55, 369, 478, 487
TaaI ACNGT 4 cut(s) 259, 718, 1012, 1078
TaiI ACGT 1 cut(s) 670
TaqI TCGA 2 cut(s) 651, 1053
TaqII GACCGA 2 cut(s) 293, 510
TasI AATT 9 cut(s) 240, 296, 350, 573, 633, 706, 997, 1177, 1220
TatI WGTACW 2 cut(s) 1091, 1245
TauI GCSGC 2 cut(s) 319, 630
TfiI GAWTC 3 cut(s) 117, 533, 686
TscAI CASTG 2 cut(s) 839, 857
TseFI GTSAC 3 cut(s) 259, 1012, 1072
TseI GCWGC 1 cut(s) 1192
Tsp45I GTSAC 3 cut(s) 259, 1012, 1072
TspDTI ATGAA 9 cut(s) 17, 189, 275, 412, 437, 546, 551, 816, 989
TspGWI ACGGA 2 cut(s) 198, 384
TspRI CASTG 2 cut(s) 839, 857
Tth111I GACNNNGTC 1 cut(s) 257
VpaK11BI GGWCC 3 cut(s) 440, 829, 1040
XapI RAATTY 3 cut(s) 573, 997, 1177
XmiI GTMKAC 2 cut(s) 160, 720
XmnI GAANNNNTTC 1 cut(s) 473
XspI CTAG 2 cut(s) 1032, 1249
ZrmI AGTACT 2 cut(s) 1093, 1247
Zsp2I ATGCAT 1 cut(s) 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.