FvH4_4g26973

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001447
Physical Location & Seq
Reverse (-)
28152862 .. 28155381
2520 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26973.t1

Sequence Viewer

Length: 1209 bp
ATGAAGGGTGGTAGTGGTCAGAATCTGAGCACTGTAAAATTTGTTCCGAAGTTCATCAATGTTCAAATCCACCATCTCGTCAATGTTCTCTCTTATTTCCTCTTATTTGGTGTTGGAATAACCCTTGGGATGTTCCTCACTGTCTATCTCAAAGACTTTTCATTCAGTCTCCAATTGGCCCAATTCTCATTCACAACTTCTTCATCAAATCAAAACATGATAATGCCAAGTGTGACTGCGGCACAAACCAATTTGAGTCACCCCCAACACATTGGATTGGAGAAGTTTCTGAAGCCCCCTATGATTTTGCACGACATGGATGATGATGAATTGCTATGGAGAGCTTCTTTGGCTCCTCGGATCTCAGAGTATCCATTCCATCGAGTTCCGAAAGTTGCCTTCTTGTTCTTGACCAAGGGACCTGTTTATTTGGCTCCATTGTGGGAGACGTTTTTCAAAGGGCATCGAGGTTTGTACTCGATTTATGTGCACTCGAATCCGTCTTACAATGGATCGCACCCGGAAAGTCCAGTGTTTCAGGGCAGAAGAATTCCTAGTCAGGAAGTAGAATGGGGAAATGTGAACATGATTGAGGCCGAGCGCCGCCTATTGGCAAATGCGCTTCTTGATATCTCAAATGAACGCTTTGTTCTTCTCTCGGAATCATGCATCCCCCTGCACAACTTCAACACCATCTACCATCACCTTGTGAATTCAAACAAATCATTTGTTGAAGTCTATGACGATCCAAGTAGTGTTGGCCGTGGGAGATACAATTCACACATGTACCCGCGTATCACATTGTCACAATGGAGGAAAGGGTCTCAGTGGTTCGAGATGAATAGAGACCTTGCCATTGAAGTTGTTGCAGACAAAAAGTACTTCCCTCTTTTCAAGAAGTACTGCAGGGGCTCATGCTATGCAGACGAGCATTACTTGCCAACATTTGTTCAAATGAAATTTGCAGCAAAGAACTCAAATAAAACCTTGACCTGGTACGATTTTTCGAAGGGTGGACCGCACCCTGCTACGTTCACCAGAACAGACGTGACGGTTGATATGCTGAACAGCTTGAGGAATGGTACCAAATGCTTGGATTATCTAATGACTGGCAACACTTGTCATCACTTATTTGCTAGGAAGTTCACTCCTGGCACTTTGGAGAGACTGATGCGGTTTGCACCAAAACTGATGCAGTTCAACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

403

Amino Acids

46.31

Weight (kDa)

9.37

Isoelectric Point (pI)

33.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 132 - 358 1.7e-79 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1082
AccB1I GGYRCC 1 cut(s) 1082
AccII CGCG 1 cut(s) 793
AciI CCGC 5 cut(s) 239, 604, 791, 1019, 1174
AclWI GGATC 3 cut(s) 368, 520, 740
AcoI YGGCCR 1 cut(s) 760
AcsI RAATTY 4 cut(s) 38, 549, 712, 959
AcuI CTGAAG 1 cut(s) 311
AdeI CACNNNGTG 1 cut(s) 709
AfaI GTAC 6 cut(s) 476, 788, 881, 902, 998, 1084
AfiI CCNNNNNNNGG 2 cut(s) 610, 993
AflIII ACRYGT 1 cut(s) 783
AgsI TTSAA 9 cut(s) 65, 457, 688, 717, 734, 860, 895, 953, 1201
AjiI CACGTC 1 cut(s) 1048
AjnI CCWGG 2 cut(s) 992, 1150
AluBI AGCT 2 cut(s) 344, 1071
AluI AGCT 2 cut(s) 344, 1071
Alw21I GWGCWC 2 cut(s) 32, 492
Alw26I GTCTC 5 cut(s) 173, 440, 828, 840, 1159
Alw44I GTGCAC 1 cut(s) 488
AlwI GGATC 3 cut(s) 368, 520, 740
AlwNI CAGNNNCTG 1 cut(s) 25
AoxI GGCC 3 cut(s) 177, 594, 760
ApaLI GTGCAC 1 cut(s) 488
ApeKI GCWGC 1 cut(s) 965
ApoI RAATTY 4 cut(s) 38, 549, 712, 959
Asp718I GGTACC 1 cut(s) 1082
AspLEI GCGC 2 cut(s) 603, 622
AspS9I GGNCC 3 cut(s) 178, 419, 1016
AsuC2I CCSGG 1 cut(s) 521
AsuHPI GGTGA 3 cut(s) 251, 695, 1027
AsuII TTCGAA 1 cut(s) 1007
AvaII GGWCC 2 cut(s) 419, 1016
BaeGI GKGCMC 1 cut(s) 492
BaeI ACNNNNGTAYC 4 cut(s) 770, 778, 803, 811
BanI GGYRCC 1 cut(s) 1082
BanII GRGCYC 1 cut(s) 914
Bbv12I GWGCWC 2 cut(s) 32, 492
BbvI GCAGC 1 cut(s) 977
BccI CCATC 4 cut(s) 81, 387, 701, 708
BceAI ACGGC 1 cut(s) 747
BcgI CGANNNNNNTGC 2 cut(s) 469, 503
BciT130I CCWGG 2 cut(s) 994, 1152
BciVI GTATCC 1 cut(s) 381
BcnI CCSGG 1 cut(s) 521
BcoDI GTCTC 5 cut(s) 173, 440, 828, 840, 1159
BfaI CTAG 2 cut(s) 555, 1137
BfmI CTRYAG 1 cut(s) 904
BfoI RGCGCY 1 cut(s) 604
BfuI GTATCC 1 cut(s) 381
BisI GCNGC 3 cut(s) 240, 604, 966
BlsI GCNGC 3 cut(s) 241, 605, 967
BmcAI AGTACT 2 cut(s) 881, 902
Bme1390I CCNGG 3 cut(s) 521, 994, 1152
Bme18I GGWCC 2 cut(s) 419, 1016
BmgBI CACGTC 1 cut(s) 1048
BmgT120I GGNCC 3 cut(s) 178, 419, 1016
BmiI GGNNCC 4 cut(s) 354, 420, 435, 1084
BmrFI CCNGG 3 cut(s) 521, 994, 1152
BmsI GCATC 4 cut(s) 472, 678, 1161, 1182
Bpu14I TTCGAA 1 cut(s) 1007
BpuEI CTTGAG 1 cut(s) 1093
BpuMI CCSGG 1 cut(s) 521
BsaI GGTCTC 2 cut(s) 828, 840
BsaJI CCNNGG 4 cut(s) 124, 356, 414, 763
Bsc4I CCNNNNNNNGG 2 cut(s) 610, 993
Bse1I ACTGG 2 cut(s) 530, 1114
BseBI CCWGG 2 cut(s) 994, 1152
BseDI CCNNGG 4 cut(s) 124, 356, 414, 763
BseGI GGATG 3 cut(s) 135, 325, 669
BseLI CCNNNNNNNGG 2 cut(s) 610, 993
BseMII CTCAG 3 cut(s) 17, 378, 839
BseNI ACTGG 2 cut(s) 530, 1114
BseRI GAGGAG 1 cut(s) 345
BseSI GKGCMC 1 cut(s) 492
BseXI GCAGC 1 cut(s) 977
BsgI GTGCAG 1 cut(s) 662
Bsh1236I CGCG 1 cut(s) 793
BshFI GGCC 3 cut(s) 179, 596, 762
BshNI GGYRCC 1 cut(s) 1082
BsiHKAI GWGCWC 2 cut(s) 32, 492
BsiSI CCGG 1 cut(s) 521
BslFI GGGAC 1 cut(s) 432
BslI CCNNNNNNNGG 2 cut(s) 610, 993
BsmAI GTCTC 5 cut(s) 173, 440, 828, 840, 1159
BsmBI CGTCTC 1 cut(s) 440
BsmFI GGGAC 1 cut(s) 432
BsnI GGCC 3 cut(s) 179, 596, 762
Bso31I GGTCTC 2 cut(s) 828, 840
Bsp119I TTCGAA 1 cut(s) 1007
Bsp1286I GDGCHC 3 cut(s) 32, 492, 914
Bsp143I GATC 3 cut(s) 360, 512, 745
BspACI CCGC 5 cut(s) 239, 604, 791, 1019, 1174
BspANI GGCC 3 cut(s) 179, 596, 762
BspCNI CTCAG 3 cut(s) 18, 377, 838
BspFNI CGCG 1 cut(s) 793
BspLI GGNNCC 4 cut(s) 354, 420, 435, 1084
BspMAI CTGCAG 1 cut(s) 908
BspPI GGATC 3 cut(s) 368, 520, 740
BspT104I TTCGAA 1 cut(s) 1007
BspT107I GGYRCC 1 cut(s) 1082
BspTNI GGTCTC 2 cut(s) 828, 840
BsrI ACTGG 2 cut(s) 530, 1114
BssECI CCNNGG 4 cut(s) 124, 356, 414, 763
BssMI GATC 3 cut(s) 360, 512, 745
BssT1I CCWWGG 2 cut(s) 124, 414
Bst2UI CCWGG 2 cut(s) 994, 1152
Bst4CI ACNGT 3 cut(s) 34, 142, 1054
BstAPI GCANNNNNTGC 1 cut(s) 937
BstBI TTCGAA 1 cut(s) 1007
BstDEI CTNAG 3 cut(s) 26, 364, 825
BstDSI CCRYGG 1 cut(s) 763
BstF5I GGATG 3 cut(s) 135, 325, 669
BstFNI CGCG 1 cut(s) 793
BstH2I RGCGCY 1 cut(s) 604
BstHHI GCGC 2 cut(s) 603, 622
BstKTI GATC 3 cut(s) 363, 515, 748
BstMAI GTCTC 5 cut(s) 173, 440, 828, 840, 1159
BstMBI GATC 3 cut(s) 360, 512, 745
BstMWI GCNNNNNNNGC 2 cut(s) 350, 937
BstNI CCWGG 2 cut(s) 994, 1152
BstNSI RCATGY 1 cut(s) 787
BstSCI CCNGG 3 cut(s) 519, 992, 1150
BstSFI CTRYAG 1 cut(s) 904
BstSLI GKGCMC 1 cut(s) 492
BstUI CGCG 1 cut(s) 793
BstV1I GCAGC 1 cut(s) 977
BstX2I RGATCY 1 cut(s) 360
BstXI CCANNNNNNTGG 2 cut(s) 272, 1093
BstYI RGATCY 1 cut(s) 360
BsuI GTATCC 1 cut(s) 381
BsuRI GGCC 3 cut(s) 179, 596, 762
BtgI CCRYGG 1 cut(s) 763
BtrI CACGTC 1 cut(s) 1048
BtsCI GGATG 3 cut(s) 135, 325, 669
BtsIMutI CAGTG 4 cut(s) 30, 138, 537, 833
CaiI CAGNNNCTG 1 cut(s) 25
CfoI GCGC 2 cut(s) 603, 622
Cfr13I GGNCC 3 cut(s) 178, 419, 1016
CsiI ACCWGGT 1 cut(s) 992
Csp6I GTAC 6 cut(s) 475, 787, 880, 901, 997, 1083
CviAII CATG 6 cut(s) 217, 316, 586, 666, 784, 915
CviJI RGCY 9 cut(s) 179, 295, 344, 353, 434, 596, 762, 912, 1071
CviKI_1 RGCY 9 cut(s) 179, 295, 344, 353, 434, 596, 762, 912, 1071
CviQI GTAC 6 cut(s) 475, 787, 880, 901, 997, 1083
DdeI CTNAG 3 cut(s) 26, 364, 825
DpnI GATC 3 cut(s) 362, 514, 747
DpnII GATC 3 cut(s) 360, 512, 745
DraIII CACNNNGTG 1 cut(s) 709
EaeI YGGCCR 1 cut(s) 760
Eco130I CCWWGG 2 cut(s) 124, 414
Eco24I GRGCYC 1 cut(s) 914
Eco31I GGTCTC 2 cut(s) 828, 840
Eco32I GATATC 1 cut(s) 631
Eco47I GGWCC 2 cut(s) 419, 1016
Eco57I CTGAAG 1 cut(s) 311
EcoO109I RGGNCCY 1 cut(s) 419
EcoRI GAATTC 2 cut(s) 549, 712
EcoRII CCWGG 2 cut(s) 992, 1150
EcoRV GATATC 1 cut(s) 631
EcoT14I CCWWGG 2 cut(s) 124, 414
EcoT22I ATGCAT 1 cut(s) 671
EcoT38I GRGCYC 1 cut(s) 914
ErhI CCWWGG 2 cut(s) 124, 414
Esp3I CGTCTC 1 cut(s) 440
FaeI CATG 6 cut(s) 220, 319, 589, 669, 787, 918
FaqI GGGAC 1 cut(s) 432
FatI CATG 6 cut(s) 216, 315, 585, 665, 783, 914
FauI CCCGC 1 cut(s) 798
Fnu4HI GCNGC 3 cut(s) 240, 604, 966
FokI GGATG 3 cut(s) 142, 332, 656
FriOI GRGCYC 1 cut(s) 914
Fsp4HI GCNGC 3 cut(s) 240, 604, 966
FspBI CTAG 2 cut(s) 555, 1137
GlaI GCGC 2 cut(s) 602, 621
GluI GCNGC 3 cut(s) 240, 604, 966
HaeII RGCGCY 1 cut(s) 604
HaeIII GGCC 3 cut(s) 179, 596, 762
HapII CCGG 1 cut(s) 521
HhaI GCGC 2 cut(s) 603, 622
Hin1II CATG 6 cut(s) 220, 319, 589, 669, 787, 918
Hin6I GCGC 2 cut(s) 601, 620
HinP1I GCGC 2 cut(s) 601, 620
HinfI GANTC 4 cut(s) 22, 256, 496, 662
HpaII CCGG 1 cut(s) 521
HphI GGTGA 3 cut(s) 251, 695, 1027
Hpy166II GTNNAC 5 cut(s) 490, 583, 1016, 1035, 1146
Hpy188I TCNGA 8 cut(s) 21, 27, 48, 291, 360, 367, 390, 661
Hpy188III TCNNGA 5 cut(s) 409, 560, 626, 835, 895
Hpy8I GTNNAC 5 cut(s) 490, 583, 1016, 1035, 1146
HpyAV CCTTC 2 cut(s) 409, 1003
HpyCH4III ACNGT 3 cut(s) 34, 142, 1054
HpyCH4IV ACGT 3 cut(s) 449, 1031, 1047
HpyF10VI GCNNNNNNNGC 2 cut(s) 350, 937
HpyF3I CTNAG 3 cut(s) 26, 364, 825
HpySE526I ACGT 3 cut(s) 449, 1031, 1047
Hsp92II CATG 6 cut(s) 220, 319, 589, 669, 787, 918
HspAI GCGC 2 cut(s) 601, 620
KpnI GGTACC 1 cut(s) 1086
Kzo9I GATC 3 cut(s) 360, 512, 745
LmnI GCTCC 2 cut(s) 358, 439
Lsp1109I GCAGC 1 cut(s) 977
LweI GCATC 4 cut(s) 472, 678, 1161, 1182
MabI ACCWGGT 1 cut(s) 992
MaeI CTAG 2 cut(s) 555, 1137
MaeII ACGT 3 cut(s) 449, 1031, 1047
MaeIII GTNAC 4 cut(s) 232, 257, 804, 1048
MalI GATC 3 cut(s) 362, 514, 747
MboI GATC 3 cut(s) 360, 512, 745
MboII GAAGA 3 cut(s) 192, 558, 644
MfeI CAATTG 1 cut(s) 173
MflI RGATCY 1 cut(s) 360
MhlI GDGCHC 3 cut(s) 32, 492, 914
MluCI AATT 9 cut(s) 38, 173, 182, 250, 329, 549, 712, 775, 959
MlyI GAGTC 1 cut(s) 265
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 8 cut(s) 110, 146, 366, 461, 586, 807, 897, 1068
Mph1103I ATGCAT 1 cut(s) 671
MslI CAYNNNNRTG 1 cut(s) 221
MspI CCGG 1 cut(s) 521
MspR9I CCNGG 3 cut(s) 521, 994, 1152
MunI CAATTG 1 cut(s) 173
MvaI CCWGG 2 cut(s) 994, 1152
MvnI CGCG 1 cut(s) 793
MwoI GCNNNNNNNGC 2 cut(s) 350, 937
NciI CCSGG 1 cut(s) 521
NdeII GATC 3 cut(s) 360, 512, 745
NlaIII CATG 6 cut(s) 220, 319, 589, 669, 787, 918
NlaIV GGNNCC 4 cut(s) 354, 420, 435, 1084
NmeAIII GCCGAG 1 cut(s) 622
NmuCI GTSAC 4 cut(s) 232, 257, 804, 1048
NsiI ATGCAT 1 cut(s) 671
NspI RCATGY 1 cut(s) 787
NspV TTCGAA 1 cut(s) 1007
PciI ACATGT 1 cut(s) 783
PfeI GAWTC 3 cut(s) 22, 496, 662
PkrI GCNGC 3 cut(s) 241, 605, 967
PleI GAGTC 1 cut(s) 264
PpsI GAGTC 1 cut(s) 264
PpuMI RGGWCCY 1 cut(s) 419
PscI ACATGT 1 cut(s) 783
Psp5II RGGWCCY 1 cut(s) 419
Psp6I CCWGG 2 cut(s) 992, 1150
PspGI CCWGG 2 cut(s) 992, 1150
PspN4I GGNNCC 4 cut(s) 354, 420, 435, 1084
PspPI GGNCC 3 cut(s) 178, 419, 1016
PspPPI RGGWCCY 1 cut(s) 419
PsrI GAACNNNNNNTAC 2 cut(s) 27, 59
PstI CTGCAG 1 cut(s) 908
PstNI CAGNNNCTG 1 cut(s) 25
PsuI RGATCY 1 cut(s) 360
RsaI GTAC 6 cut(s) 476, 788, 881, 902, 998, 1084
RsaNI GTAC 6 cut(s) 475, 787, 880, 901, 997, 1083
RseI CAYNNNNRTG 1 cut(s) 221
SatI GCNGC 3 cut(s) 240, 604, 966
Sau3AI GATC 3 cut(s) 360, 512, 745
Sau96I GGNCC 3 cut(s) 178, 419, 1016
ScaI AGTACT 2 cut(s) 881, 902
SchI GAGTC 1 cut(s) 265
ScrFI CCNGG 3 cut(s) 521, 994, 1152
SduI GDGCHC 3 cut(s) 32, 492, 914
SexAI ACCWGGT 1 cut(s) 992
SfaNI GCATC 4 cut(s) 472, 678, 1161, 1182
SfcI CTRYAG 1 cut(s) 904
SfuI TTCGAA 1 cut(s) 1007
SinI GGWCC 2 cut(s) 419, 1016
SmiMI CAYNNNNRTG 1 cut(s) 221
SmlI CTYRAG 1 cut(s) 1072
SmoI CTYRAG 1 cut(s) 1072
Sse9I AATT 9 cut(s) 38, 173, 182, 250, 329, 549, 712, 775, 959
SsiI CCGC 5 cut(s) 239, 604, 791, 1019, 1174
SspMI CTAG 2 cut(s) 555, 1137
StyD4I CCNGG 3 cut(s) 519, 992, 1150
StyI CCWWGG 2 cut(s) 124, 414
TaaI ACNGT 3 cut(s) 34, 142, 1054
TaiI ACGT 3 cut(s) 452, 1034, 1050
TaqI TCGA 6 cut(s) 382, 466, 479, 494, 834, 1007
TasI AATT 9 cut(s) 38, 173, 182, 250, 329, 549, 712, 775, 959
TatI WGTACW 3 cut(s) 474, 879, 900
TauI GCSGC 2 cut(s) 242, 606
TfiI GAWTC 3 cut(s) 22, 496, 662
TscAI CASTG 4 cut(s) 37, 145, 537, 833
TseFI GTSAC 4 cut(s) 232, 257, 804, 1048
TseI GCWGC 1 cut(s) 965
Tsp45I GTSAC 4 cut(s) 232, 257, 804, 1048
TspDTI ATGAA 8 cut(s) 17, 43, 150, 192, 342, 654, 854, 971
TspGWI ACGGA 1 cut(s) 489
TspRI CASTG 4 cut(s) 37, 145, 537, 833
VneI GTGCAC 1 cut(s) 488
VpaK11BI GGWCC 2 cut(s) 419, 1016
XapI RAATTY 4 cut(s) 38, 549, 712, 959
XceI RCATGY 1 cut(s) 787
XspI CTAG 2 cut(s) 555, 1137
ZrmI AGTACT 2 cut(s) 881, 902
Zsp2I ATGCAT 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.