MD13G1038600.v1.1

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001447
Physical Location & Seq
Reverse (-)
2620952 .. 2622888
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1038600.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGAAGGCTCAAGATCCAAACTCTCCAATCTTTTTTCCAAAATCCTTCAATCTCCAACGCCACCTCCTCAATCTGCTCTCATATTTCCTGCTCTTCCTTTTCGGAGTAACCCTCGGAGTTTTACTTAGCAATCTCAATGACTTTTCATTTGGCCGTCTACAGTTCGCACAGCTCCCCTTCTCACCTATCTCACCTCCTCCTCCATCCAATATTTCAAGAACCCGAATGCCAAATGCAACCTCCCCAACAACATCTGTTTCGTCAAGTCCTCCTCGGACAGGGTTGAAGGATTATCTGAAGCCACCGAAGGCATTTCACGACATGAACAATGCAGAACTGCTGTGGAGAGCTTCTATAACTCCGCGGATTCCTGAATATCCATTCCATTTTGTTCCAAAAGTTGCTTTCATGTTCTTGACAAAGGGCCCTGTTGTTTTGGCACCACTGTGGGAGAAGTTCTTTGCCGGGCATCAAGGTTTGTACTCAATTTATGTGCACTCAAGTCCGTCCTACAATCACTCATTGTACACCGAAACCCCAGTTTTTGGTGGCCGGCGAATTCCAAGTCAGGAAGTAGAATGGGGGAAGGTGAGCCTGATCGCGGCAGAGCGCCTCCTCCTGGCGAATGCTCTTCTCGACGTCTCAAACCAGCGTTTTGTTCTGCTCTCAGAATCATGCATTCCCCTCTATAACTTCACCACTGTCTACTCCTACCTCATGAACTCCACCCAAACTTTCGTCGAGGTCTTTGACGATCCCAGTGGAGTAGGACGCGGCCGGTATGAACTGAGTGACTACCTGGGAATTACAATAGATCAGTGGCGAAAAGGGTCGCAGTGGTTCCAAGCTGATCGAGACCTCGCCCTTGAATTCGTATCGGATCGAATATACTTCCCAGTGTTCATGAAATGCAAAGGCTACTGTTTCGCCGATGAACATTATTTGCCAACGTTTGTGTACATAAAATTTGGAGCAAAGAATGCAAATAGGACGTTGACCTGGGTTGACTGGGAAAAGGGTGGCCCACACCCTACGGAGTACGCGAGCGAAAATGTGACGGCAGTGCTTTTGAGTGGCCTGAGGAATGGTTTTGGTCGGAAATGTGAATACAATGGGAGGACTACAGATGTATGTTTCTTATTCGCCAGGAAGTTCCCGCCAACTGCTTTGGATAACCTACTGAAGTTTGCACCGGAGATCATGCACTTCAACACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

406

Amino Acids

46.13

Weight (kDa)

8.92

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 134 - 358 4.5e-71 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 642
AccB1I GGYRCC 1 cut(s) 439
AccB7I CCANNNNNTGG 1 cut(s) 545
AccI GTMKAC 2 cut(s) 157, 705
AccII CGCG 4 cut(s) 364, 602, 774, 1043
AciI CCGC 5 cut(s) 362, 364, 602, 774, 1157
AclI AACGTT 1 cut(s) 950
AclWI GGATC 3 cut(s) 8, 749, 888
AcoI YGGCCR 3 cut(s) 151, 550, 775
AcsI RAATTY 3 cut(s) 558, 869, 965
AcuI CTGAAG 2 cut(s) 317, 1202
AcyI GRCGYC 1 cut(s) 639
AfaI GTAC 4 cut(s) 482, 527, 959, 1040
AfiI CCNNNNNNNGG 4 cut(s) 278, 545, 601, 619
AgsI TTSAA 5 cut(s) 49, 216, 286, 869, 1210
AjnI CCWGG 4 cut(s) 618, 798, 998, 1145
AleI CACNNNNGTG 1 cut(s) 445
AluBI AGCT 3 cut(s) 172, 350, 848
AluI AGCT 3 cut(s) 172, 350, 848
Alw21I GWGCWC 1 cut(s) 498
Alw26I GTCTC 2 cut(s) 646, 849
Alw44I GTGCAC 1 cut(s) 494
AlwI GGATC 3 cut(s) 8, 749, 888
AoxI GGCC 6 cut(s) 151, 424, 550, 775, 1021, 1075
ApaI GGGCCC 1 cut(s) 428
ApaLI GTGCAC 1 cut(s) 494
ApoI RAATTY 3 cut(s) 558, 869, 965
ArsI GACNNNNNNTTYG 4 cut(s) 131, 163, 550, 582
AspLEI GCGC 1 cut(s) 612
AspS9I GGNCC 3 cut(s) 424, 425, 1022
AsuC2I CCSGG 1 cut(s) 466
AsuHPI GGTGA 4 cut(s) 174, 183, 601, 688
AxyI CCTNAGG 1 cut(s) 1079
BaeGI GKGCMC 2 cut(s) 428, 498
BanI GGYRCC 1 cut(s) 439
BanII GRGCYC 1 cut(s) 428
Bbv12I GWGCWC 1 cut(s) 498
BccI CCATC 1 cut(s) 211
BceAI ACGGC 2 cut(s) 138, 1074
BciT130I CCWGG 4 cut(s) 620, 800, 1000, 1147
BcnI CCSGG 1 cut(s) 466
BcoDI GTCTC 2 cut(s) 646, 849
BfmI CTRYAG 2 cut(s) 158, 1122
BfoI RGCGCY 1 cut(s) 613
BisI GCNGC 2 cut(s) 603, 775
BlsI GCNGC 2 cut(s) 604, 776
Bme1390I CCNGG 5 cut(s) 466, 620, 800, 1000, 1147
BmgT120I GGNCC 3 cut(s) 424, 425, 1022
BmiI GGNNCC 3 cut(s) 426, 441, 842
BmrFI CCNGG 5 cut(s) 466, 620, 800, 1000, 1147
BmrI ACTGGG 4 cut(s) 533, 753, 890, 1018
BmsI GCATC 1 cut(s) 478
BmuI ACTGGG 4 cut(s) 533, 753, 890, 1018
BplI GAGNNNNNCTC 2 cut(s) 96, 128
BpuEI CTTGAG 1 cut(s) 484
BpuMI CCSGG 1 cut(s) 466
BsaHI GRCGYC 1 cut(s) 639
BsaI GGTCTC 1 cut(s) 849
BsaJI CCNNGG 5 cut(s) 112, 272, 362, 799, 999
BsaWI WCCGGW 1 cut(s) 1192
BsaXI ACNNNNNCTCC 2 cut(s) 48, 78
Bsc4I CCNNNNNNNGG 4 cut(s) 278, 545, 601, 619
Bse118I RCCGGY 2 cut(s) 552, 777
Bse1I ACTGG 4 cut(s) 539, 759, 896, 1013
Bse21I CCTNAGG 1 cut(s) 1079
BseBI CCWGG 4 cut(s) 620, 800, 1000, 1147
BseDI CCNNGG 5 cut(s) 112, 272, 362, 799, 999
BseGI GGATG 1 cut(s) 203
BseLI CCNNNNNNNGG 4 cut(s) 278, 545, 601, 619
BseMII CTCAG 3 cut(s) 681, 779, 1070
BseNI ACTGG 4 cut(s) 539, 759, 896, 1013
BseRI GAGGAG 5 cut(s) 56, 186, 189, 261, 605
BseSI GKGCMC 2 cut(s) 428, 498
BseX3I CGGCCG 1 cut(s) 775
Bsh1236I CGCG 4 cut(s) 364, 602, 774, 1043
Bsh1285I CGRYCG 1 cut(s) 778
BshFI GGCC 6 cut(s) 153, 426, 552, 777, 1023, 1077
BshNI GGYRCC 1 cut(s) 439
BsiEI CGRYCG 1 cut(s) 778
BsiHKAI GWGCWC 1 cut(s) 498
BsiSI CCGG 4 cut(s) 465, 553, 778, 1193
BslI CCNNNNNNNGG 4 cut(s) 278, 545, 601, 619
BsmAI GTCTC 2 cut(s) 646, 849
BsmBI CGTCTC 1 cut(s) 646
BsmI GAATGC 4 cut(s) 231, 631, 678, 985
BsnI GGCC 6 cut(s) 153, 426, 552, 777, 1023, 1077
Bso31I GGTCTC 1 cut(s) 849
Bsp120I GGGCCC 1 cut(s) 424
Bsp1286I GDGCHC 2 cut(s) 428, 498
Bsp1407I TGTACA 2 cut(s) 525, 957
Bsp143I GATC 7 cut(s) 13, 597, 754, 814, 850, 880, 1197
BspACI CCGC 5 cut(s) 362, 364, 602, 774, 1157
BspANI GGCC 6 cut(s) 153, 426, 552, 777, 1023, 1077
BspCNI CTCAG 3 cut(s) 680, 780, 1071
BspFNI CGCG 4 cut(s) 364, 602, 774, 1043
BspHI TCATGA 2 cut(s) 717, 903
BspLI GGNNCC 3 cut(s) 426, 441, 842
BspPI GGATC 3 cut(s) 8, 749, 888
BspQI GCTCTTC 2 cut(s) 98, 636
BspT107I GGYRCC 1 cut(s) 439
BspTNI GGTCTC 1 cut(s) 849
BsrFI RCCGGY 2 cut(s) 552, 777
BsrGI TGTACA 2 cut(s) 525, 957
BsrI ACTGG 4 cut(s) 539, 759, 896, 1013
BssAI RCCGGY 2 cut(s) 552, 777
BssECI CCNNGG 5 cut(s) 112, 272, 362, 799, 999
BssMI GATC 7 cut(s) 13, 597, 754, 814, 850, 880, 1197
BssNI GRCGYC 1 cut(s) 639
Bst2UI CCWGG 4 cut(s) 620, 800, 1000, 1147
Bst4CI ACNGT 4 cut(s) 162, 447, 703, 923
Bst6I CTCTTC 2 cut(s) 98, 636
BstACI GRCGYC 1 cut(s) 639
BstAPI GCANNNNNTGC 1 cut(s) 980
BstAUI TGTACA 2 cut(s) 525, 957
BstC8I GCNNGC 2 cut(s) 554, 1045
BstDEI CTNAG 4 cut(s) 125, 667, 788, 1079
BstDSI CCRYGG 1 cut(s) 362
BstENI CCTNNNNNAGG 1 cut(s) 276
BstF5I GGATG 1 cut(s) 203
BstFNI CGCG 4 cut(s) 364, 602, 774, 1043
BstH2I RGCGCY 1 cut(s) 613
BstHHI GCGC 1 cut(s) 612
BstKTI GATC 7 cut(s) 16, 600, 757, 817, 853, 883, 1200
BstMAI GTCTC 2 cut(s) 646, 849
BstMBI GATC 7 cut(s) 13, 597, 754, 814, 850, 880, 1197
BstMCI CGRYCG 1 cut(s) 778
BstMWI GCNNNNNNNGC 1 cut(s) 980
BstNI CCWGG 4 cut(s) 620, 800, 1000, 1147
BstSCI CCNGG 5 cut(s) 464, 618, 798, 998, 1145
BstSFI CTRYAG 2 cut(s) 158, 1122
BstSLI GKGCMC 2 cut(s) 428, 498
BstUI CGCG 4 cut(s) 364, 602, 774, 1043
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BstZI CGGCCG 1 cut(s) 775
Bsu36I CCTNAGG 1 cut(s) 1079
BsuRI GGCC 6 cut(s) 153, 426, 552, 777, 1023, 1077
BtgI CCRYGG 1 cut(s) 362
BtsCI GGATG 1 cut(s) 203
BtsI GCAGTG 2 cut(s) 842, 1068
BtsIMutI CAGTG 7 cut(s) 443, 699, 766, 824, 842, 903, 1068
Cac8I GCNNGC 2 cut(s) 554, 1045
CciI TCATGA 2 cut(s) 717, 903
CfoI GCGC 1 cut(s) 612
Cfr10I RCCGGY 2 cut(s) 552, 777
Cfr13I GGNCC 3 cut(s) 424, 425, 1022
Cfr42I CCGCGG 1 cut(s) 365
CseI GACGC 1 cut(s) 780
Csp6I GTAC 4 cut(s) 481, 526, 958, 1039
CviAII CATG 6 cut(s) 322, 409, 675, 718, 904, 1201
CviQI GTAC 4 cut(s) 481, 526, 958, 1039
DdeI CTNAG 4 cut(s) 125, 667, 788, 1079
DpnI GATC 7 cut(s) 15, 599, 756, 816, 852, 882, 1199
DpnII GATC 7 cut(s) 13, 597, 754, 814, 850, 880, 1197
EaeI YGGCCR 3 cut(s) 151, 550, 775
EagI CGGCCG 1 cut(s) 775
Eam1104I CTCTTC 2 cut(s) 98, 636
EarI CTCTTC 2 cut(s) 98, 636
EclXI CGGCCG 1 cut(s) 775
Eco24I GRGCYC 1 cut(s) 428
Eco31I GGTCTC 1 cut(s) 849
Eco52I CGGCCG 1 cut(s) 775
Eco57I CTGAAG 2 cut(s) 317, 1202
Eco81I CCTNAGG 1 cut(s) 1079
EcoNI CCTNNNNNAGG 1 cut(s) 276
EcoO109I RGGNCCY 2 cut(s) 424, 425
EcoRI GAATTC 2 cut(s) 558, 869
EcoRII CCWGG 4 cut(s) 618, 798, 998, 1145
EcoT22I ATGCAT 1 cut(s) 680
EcoT38I GRGCYC 1 cut(s) 428
Esp3I CGTCTC 1 cut(s) 646
FaeI CATG 6 cut(s) 325, 412, 678, 721, 907, 1204
FatI CATG 6 cut(s) 321, 408, 674, 717, 903, 1200
FauI CCCGC 1 cut(s) 1164
FblI GTMKAC 2 cut(s) 157, 705
Fnu4HI GCNGC 2 cut(s) 603, 775
FokI GGATG 1 cut(s) 190
FriOI GRGCYC 1 cut(s) 428
Fsp4HI GCNGC 2 cut(s) 603, 775
GlaI GCGC 1 cut(s) 611
GluI GCNGC 2 cut(s) 603, 775
HaeII RGCGCY 1 cut(s) 613
HaeIII GGCC 6 cut(s) 153, 426, 552, 777, 1023, 1077
HapII CCGG 4 cut(s) 465, 553, 778, 1193
HgaI GACGC 1 cut(s) 780
HhaI GCGC 1 cut(s) 612
Hin1I GRCGYC 1 cut(s) 639
Hin1II CATG 6 cut(s) 325, 412, 678, 721, 907, 1204
Hin6I GCGC 1 cut(s) 610
HinP1I GCGC 1 cut(s) 610
HincII GTYRAC 2 cut(s) 996, 1006
HindII GTYRAC 2 cut(s) 996, 1006
HinfI GANTC 2 cut(s) 367, 671
HpaII CCGG 4 cut(s) 465, 553, 778, 1193
HphI GGTGA 4 cut(s) 174, 183, 601, 688
Hpy166II GTNNAC 7 cut(s) 158, 496, 528, 706, 958, 996, 1006
Hpy188I TCNGA 7 cut(s) 104, 116, 276, 297, 670, 880, 1098
Hpy8I GTNNAC 7 cut(s) 158, 496, 528, 706, 958, 996, 1006
Hpy99I CGWCG 2 cut(s) 641, 743
HpyAV CCTTC 5 cut(s) 55, 187, 280, 301, 580
HpyCH4III ACNGT 4 cut(s) 162, 447, 703, 923
HpyCH4IV ACGT 3 cut(s) 639, 950, 992
HpyCH4V TGCA 8 cut(s) 236, 332, 496, 678, 912, 983, 1190, 1204
HpyF10VI GCNNNNNNNGC 1 cut(s) 980
HpyF3I CTNAG 4 cut(s) 125, 667, 788, 1079
HpySE526I ACGT 3 cut(s) 639, 950, 992
Hsp92I GRCGYC 1 cut(s) 639
Hsp92II CATG 6 cut(s) 325, 412, 678, 721, 907, 1204
HspAI GCGC 1 cut(s) 610
KroI GCCGGC 1 cut(s) 552
KroNI GCCGGC 1 cut(s) 554
KspI CCGCGG 1 cut(s) 365
Kzo9I GATC 7 cut(s) 13, 597, 754, 814, 850, 880, 1197
LguI GCTCTTC 2 cut(s) 98, 636
LmnI GCTCC 2 cut(s) 177, 971
LweI GCATC 1 cut(s) 478
MaeII ACGT 3 cut(s) 639, 950, 992
MaeIII GTNAC 3 cut(s) 106, 791, 1054
MalI GATC 7 cut(s) 15, 599, 756, 816, 852, 882, 1199
MboI GATC 7 cut(s) 13, 597, 754, 814, 850, 880, 1197
MboII GAAGA 2 cut(s) 85, 623
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 2 cut(s) 428, 498
MluCI AATT 5 cut(s) 486, 558, 804, 869, 965
MmeI TCCRAC 2 cut(s) 79, 1076
Mph1103I ATGCAT 1 cut(s) 680
MroNI GCCGGC 1 cut(s) 552
MslI CAYNNNNRTG 1 cut(s) 445
MspA1I CMGCKG 1 cut(s) 364
MspI CCGG 4 cut(s) 465, 553, 778, 1193
MspR9I CCNGG 5 cut(s) 466, 620, 800, 1000, 1147
Mva1269I GAATGC 4 cut(s) 231, 631, 678, 985
MvaI CCWGG 4 cut(s) 620, 800, 1000, 1147
MvnI CGCG 4 cut(s) 364, 602, 774, 1043
MwoI GCNNNNNNNGC 1 cut(s) 980
NaeI GCCGGC 1 cut(s) 554
NciI CCSGG 1 cut(s) 466
NdeII GATC 7 cut(s) 13, 597, 754, 814, 850, 880, 1197
NgoMIV GCCGGC 1 cut(s) 552
NlaIII CATG 6 cut(s) 325, 412, 678, 721, 907, 1204
NlaIV GGNNCC 3 cut(s) 426, 441, 842
NmuCI GTSAC 2 cut(s) 791, 1054
NsiI ATGCAT 1 cut(s) 680
OliI CACNNNNGTG 1 cut(s) 445
PagI TCATGA 2 cut(s) 717, 903
PciSI GCTCTTC 2 cut(s) 98, 636
PcsI WCGNNNNNNNCGW 1 cut(s) 1040
PctI GAATGC 4 cut(s) 231, 631, 678, 985
PdiI GCCGGC 1 cut(s) 554
PfeI GAWTC 2 cut(s) 367, 671
PflMI CCANNNNNTGG 1 cut(s) 545
PkrI GCNGC 2 cut(s) 604, 776
Psp1406I AACGTT 1 cut(s) 950
Psp6I CCWGG 4 cut(s) 618, 798, 998, 1145
PspGI CCWGG 4 cut(s) 618, 798, 998, 1145
PspN4I GGNNCC 3 cut(s) 426, 441, 842
PspOMI GGGCCC 1 cut(s) 424
PspPI GGNCC 3 cut(s) 424, 425, 1022
PsuI RGATCY 1 cut(s) 13
RsaI GTAC 4 cut(s) 482, 527, 959, 1040
RsaNI GTAC 4 cut(s) 481, 526, 958, 1039
RseI CAYNNNNRTG 1 cut(s) 445
SacII CCGCGG 1 cut(s) 365
SapI GCTCTTC 2 cut(s) 98, 636
SatI GCNGC 2 cut(s) 603, 775
Sau3AI GATC 7 cut(s) 13, 597, 754, 814, 850, 880, 1197
Sau96I GGNCC 3 cut(s) 424, 425, 1022
ScrFI CCNGG 5 cut(s) 466, 620, 800, 1000, 1147
SduI GDGCHC 2 cut(s) 428, 498
SfaNI GCATC 1 cut(s) 478
SfcI CTRYAG 2 cut(s) 158, 1122
Sfr303I CCGCGG 1 cut(s) 365
SgrBI CCGCGG 1 cut(s) 365
SmiMI CAYNNNNRTG 1 cut(s) 445
SmlI CTYRAG 2 cut(s) 9, 499
SmoI CTYRAG 2 cut(s) 9, 499
Sse9I AATT 5 cut(s) 486, 558, 804, 869, 965
SsiI CCGC 5 cut(s) 362, 364, 602, 774, 1157
SspI AATATT 1 cut(s) 211
StyD4I CCNGG 5 cut(s) 464, 618, 798, 998, 1145
TaaI ACNGT 4 cut(s) 162, 447, 703, 923
TaiI ACGT 3 cut(s) 642, 953, 995
TaqI TCGA 4 cut(s) 636, 741, 853, 883
TasI AATT 5 cut(s) 486, 558, 804, 869, 965
TatI WGTACW 3 cut(s) 480, 525, 957
TauI GCSGC 2 cut(s) 605, 777
TfiI GAWTC 2 cut(s) 367, 671
TscAI CASTG 7 cut(s) 450, 706, 766, 824, 842, 903, 1068
TseFI GTSAC 2 cut(s) 791, 1054
Tsp45I GTSAC 2 cut(s) 791, 1054
TspDTI ATGAA 9 cut(s) 17, 135, 338, 397, 734, 798, 892, 920, 948
TspGWI ACGGA 2 cut(s) 495, 1049
TspRI CASTG 7 cut(s) 450, 706, 766, 824, 842, 903, 1068
Van91I CCANNNNNTGG 1 cut(s) 545
VneI GTGCAC 1 cut(s) 494
XagI CCTNNNNNAGG 1 cut(s) 276
XapI RAATTY 3 cut(s) 558, 869, 965
XmiI GTMKAC 2 cut(s) 157, 705
ZraI GACGTC 1 cut(s) 640
Zsp2I ATGCAT 1 cut(s) 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.