Rh4AG334300

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001447
Physical Location & Seq
Reverse (-)
64393048 .. 64394000
953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG334300.1

Sequence Viewer

Length: 792 bp
ATGTTCTTGACAAAGGGACCTGTACATTTGGCCCCATTGTGGGAGAAGTTCTTCAAAGGGCATCAAGGCTTGTACTCAATTTATGTTCACTCAGATCCATCTTACAATGCATCATCACACCCAGGAAGTCCTCTTTTTTCAAGGCAGAAGAATTCCAAGTCAGAAAGTAAAATGGGCACAGGTGAACATGGTAATTGCGAATGCTCTTCTCGATATCTCAAACCAGCGGTACGGTTCGTTCTCCTCTCGGAAGCATGCATTCCCCTATACAACTTCTCCACTATCTACACTTACCTCCTAAACTCCCAAGAAACTTTTGTGGAGGTCTATGATGATCCAAGTTCAGTTGGACGCGGCCGATATAATTCAAGTTACTACCCAATATTGGATCAGTGGAGGAAAGGGTCACAGTGGGTTGAAATTGATAGAGACATTGCCATTGAAGTTGTCTCGGATCGGACATACTTGCCAGCCTTCCGGTTGGGCAACCGCTCTTGTTTTGCTGATGAACAATACTCGCCAACATTTGTGAACCTAAAATTTTCGGCAAAGAATTCAAACAGGAGTGTCAACTGGGTTAATTGGTCTACGCGTGGCCCTCACCCTGTCGAGTACAATAGCACATCGGCCGAGCTTCTGAACACCCTGCGCAATGGTAATGATCAGAGATGTTTATATAATCGAAGGAGAAGCAATGTTTGTTTCTTATTCGCCAGGAAGTTCCGGCCTTCTGCTTTGGATAGCCTTCTCAGGCTTGCCCCCAAGATCATGCACTTCAACACAGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

263

Amino Acids

30.26

Weight (kDa)

9.26

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 1 - 215 2.3e-50 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 650
AccBSI CCGCTC 1 cut(s) 492
AccI GTMKAC 1 cut(s) 587
AccII CGCG 2 cut(s) 354, 592
AciI CCGC 3 cut(s) 227, 354, 490
AclWI GGATC 4 cut(s) 89, 329, 396, 462
AcoI YGGCCR 2 cut(s) 355, 627
AcsI RAATTY 3 cut(s) 151, 539, 553
AfaI GTAC 4 cut(s) 24, 74, 231, 614
AfiI CCNNNNNNNGG 3 cut(s) 39, 40, 385
AflIII ACRYGT 1 cut(s) 590
AgsI TTSAA 7 cut(s) 55, 141, 369, 419, 443, 558, 778
AjnI CCWGG 2 cut(s) 121, 713
AluBI AGCT 1 cut(s) 634
AluI AGCT 1 cut(s) 634
Alw26I GTCTC 2 cut(s) 423, 454
AlwI GGATC 4 cut(s) 89, 329, 396, 462
AoxI GGCC 5 cut(s) 30, 355, 595, 627, 725
ApoI RAATTY 3 cut(s) 151, 539, 553
Asp700I GAANNNNTTC 1 cut(s) 50
AspLEI GCGC 1 cut(s) 651
AspS9I GGNCC 3 cut(s) 17, 31, 596
AsuHPI GGTGA 2 cut(s) 194, 593
AvaII GGWCC 1 cut(s) 17
BaeGI GKGCMC 1 cut(s) 179
BccI CCATC 1 cut(s) 106
BciT130I CCWGG 2 cut(s) 123, 715
BclI TGATCA 1 cut(s) 661
BcoDI GTCTC 2 cut(s) 423, 454
BisI GCNGC 1 cut(s) 355
BlsI GCNGC 1 cut(s) 356
Bme1390I CCNGG 2 cut(s) 123, 715
Bme18I GGWCC 1 cut(s) 17
BmgT120I GGNCC 3 cut(s) 17, 31, 596
BmiI GGNNCC 2 cut(s) 18, 33
BmrFI CCNGG 2 cut(s) 123, 715
BmrI ACTGGG 1 cut(s) 583
BmsI GCATC 2 cut(s) 70, 119
BmuI ACTGGG 1 cut(s) 583
BsaJI CCNNGG 1 cut(s) 121
BsaWI WCCGGW 1 cut(s) 477
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 40, 385
Bse1I ACTGG 1 cut(s) 578
Bse3DI GCAATG 3 cut(s) 432, 658, 700
BseBI CCWGG 2 cut(s) 123, 715
BseDI CCNNGG 1 cut(s) 121
BseLI CCNNNNNNNGG 3 cut(s) 39, 40, 385
BseMI GCAATG 3 cut(s) 432, 658, 700
BseMII CTCAG 2 cut(s) 105, 763
BseNI ACTGG 1 cut(s) 578
BseRI GAGGAG 1 cut(s) 233
BseSI GKGCMC 1 cut(s) 179
BseX3I CGGCCG 2 cut(s) 355, 627
Bsh1236I CGCG 2 cut(s) 354, 592
Bsh1285I CGRYCG 2 cut(s) 358, 630
BshFI GGCC 5 cut(s) 32, 357, 597, 629, 727
BsiEI CGRYCG 2 cut(s) 358, 630
BsiSI CCGG 2 cut(s) 478, 724
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 3 cut(s) 39, 40, 385
BsmAI GTCTC 2 cut(s) 423, 454
BsmFI GGGAC 1 cut(s) 30
BsmI GAATGC 2 cut(s) 206, 258
BsnI GGCC 5 cut(s) 32, 357, 597, 629, 727
Bsp1286I GDGCHC 1 cut(s) 179
Bsp1407I TGTACA 1 cut(s) 22
Bsp143I GATC 6 cut(s) 94, 334, 388, 454, 661, 765
BspACI CCGC 3 cut(s) 227, 354, 490
BspANI GGCC 5 cut(s) 32, 357, 597, 629, 727
BspCNI CTCAG 2 cut(s) 104, 762
BspFNI CGCG 2 cut(s) 354, 592
BspLI GGNNCC 2 cut(s) 18, 33
BspPI GGATC 4 cut(s) 89, 329, 396, 462
BspQI GCTCTTC 1 cut(s) 211
BsrBI CCGCTC 1 cut(s) 492
BsrDI GCAATG 3 cut(s) 432, 658, 700
BsrGI TGTACA 1 cut(s) 22
BsrI ACTGG 1 cut(s) 578
BssECI CCNNGG 1 cut(s) 121
BssMI GATC 6 cut(s) 94, 334, 388, 454, 661, 765
Bst2UI CCWGG 2 cut(s) 123, 715
Bst4CI ACNGT 2 cut(s) 234, 411
Bst6I CTCTTC 1 cut(s) 211
BstAUI TGTACA 1 cut(s) 22
BstC8I GCNNGC 3 cut(s) 256, 471, 756
BstDEI CTNAG 2 cut(s) 91, 749
BstFNI CGCG 2 cut(s) 354, 592
BstHHI GCGC 1 cut(s) 651
BstKTI GATC 6 cut(s) 97, 337, 391, 457, 664, 768
BstMAI GTCTC 2 cut(s) 423, 454
BstMBI GATC 6 cut(s) 94, 334, 388, 454, 661, 765
BstMCI CGRYCG 2 cut(s) 358, 630
BstNI CCWGG 2 cut(s) 123, 715
BstNSI RCATGY 1 cut(s) 258
BstSCI CCNGG 2 cut(s) 121, 713
BstSLI GKGCMC 1 cut(s) 179
BstUI CGCG 2 cut(s) 354, 592
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BstZI CGGCCG 2 cut(s) 355, 627
BsuRI GGCC 5 cut(s) 32, 357, 597, 629, 727
BtsIMutI CAGTG 2 cut(s) 398, 416
Cac8I GCNNGC 3 cut(s) 256, 471, 756
CfoI GCGC 1 cut(s) 651
Cfr13I GGNCC 3 cut(s) 17, 31, 596
CseI GACGC 1 cut(s) 360
Csp6I GTAC 4 cut(s) 23, 73, 230, 613
CviAII CATG 3 cut(s) 188, 255, 769
CviQI GTAC 4 cut(s) 23, 73, 230, 613
DdeI CTNAG 2 cut(s) 91, 749
DpnI GATC 6 cut(s) 96, 336, 390, 456, 663, 767
DpnII GATC 6 cut(s) 94, 334, 388, 454, 661, 765
EaeI YGGCCR 2 cut(s) 355, 627
EagI CGGCCG 2 cut(s) 355, 627
Eam1104I CTCTTC 1 cut(s) 211
EarI CTCTTC 1 cut(s) 211
EclXI CGGCCG 2 cut(s) 355, 627
Eco32I GATATC 1 cut(s) 215
Eco47I GGWCC 1 cut(s) 17
Eco52I CGGCCG 2 cut(s) 355, 627
EcoO109I RGGNCCY 1 cut(s) 17
EcoRI GAATTC 2 cut(s) 151, 553
EcoRII CCWGG 2 cut(s) 121, 713
EcoRV GATATC 1 cut(s) 215
EcoT22I ATGCAT 2 cut(s) 112, 260
FaeI CATG 3 cut(s) 191, 258, 772
FaqI GGGAC 1 cut(s) 30
FatI CATG 3 cut(s) 187, 254, 768
FbaI TGATCA 1 cut(s) 661
FblI GTMKAC 1 cut(s) 587
Fnu4HI GCNGC 1 cut(s) 355
Fsp4HI GCNGC 1 cut(s) 355
FspI TGCGCA 1 cut(s) 650
GlaI GCGC 1 cut(s) 650
GluI GCNGC 1 cut(s) 355
HaeIII GGCC 5 cut(s) 32, 357, 597, 629, 727
HapII CCGG 2 cut(s) 478, 724
HgaI GACGC 1 cut(s) 360
HhaI GCGC 1 cut(s) 651
Hin1II CATG 3 cut(s) 191, 258, 772
Hin6I GCGC 1 cut(s) 649
HinP1I GCGC 1 cut(s) 649
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
HinfI GANTC 1 cut(s) 785
HpaII CCGG 2 cut(s) 478, 724
HphI GGTGA 2 cut(s) 194, 593
Hpy166II GTNNAC 5 cut(s) 88, 185, 532, 571, 588
Hpy188I TCNGA 7 cut(s) 94, 163, 250, 454, 459, 639, 666
Hpy188III TCNNGA 2 cut(s) 7, 210
Hpy8I GTNNAC 5 cut(s) 88, 185, 532, 571, 588
HpyAV CCTTC 4 cut(s) 484, 678, 738, 755
HpyCH4III ACNGT 2 cut(s) 234, 411
HpyCH4V TGCA 3 cut(s) 110, 258, 772
HpyF3I CTNAG 2 cut(s) 91, 749
Hsp92II CATG 3 cut(s) 191, 258, 772
HspAI GCGC 1 cut(s) 649
Ksp22I TGATCA 1 cut(s) 661
Kzo9I GATC 6 cut(s) 94, 334, 388, 454, 661, 765
LguI GCTCTTC 1 cut(s) 211
LweI GCATC 2 cut(s) 70, 119
MaeIII GTNAC 2 cut(s) 371, 405
MalI GATC 6 cut(s) 96, 336, 390, 456, 663, 767
MbiI CCGCTC 1 cut(s) 492
MboI GATC 6 cut(s) 94, 334, 388, 454, 661, 765
MboII GAAGA 3 cut(s) 43, 160, 198
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 1 cut(s) 179
MluCI AATT 8 cut(s) 78, 151, 193, 364, 420, 539, 553, 580
MluI ACGCGT 1 cut(s) 590
MmeI TCCRAC 1 cut(s) 328
MnlI CCTC 6 cut(s) 141, 254, 305, 316, 390, 609
Mph1103I ATGCAT 2 cut(s) 112, 260
MroXI GAANNNNTTC 1 cut(s) 50
MseI TTAA 2 cut(s) 579, 790
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 2 cut(s) 478, 724
MspR9I CCNGG 2 cut(s) 123, 715
Mva1269I GAATGC 2 cut(s) 206, 258
MvaI CCWGG 2 cut(s) 123, 715
MvnI CGCG 2 cut(s) 354, 592
NdeII GATC 6 cut(s) 94, 334, 388, 454, 661, 765
NlaIII CATG 3 cut(s) 191, 258, 772
NlaIV GGNNCC 2 cut(s) 18, 33
NmeAIII GCCGAG 1 cut(s) 655
NmuCI GTSAC 1 cut(s) 405
NsbI TGCGCA 1 cut(s) 650
NsiI ATGCAT 2 cut(s) 112, 260
NspI RCATGY 1 cut(s) 258
PaeI GCATGC 1 cut(s) 258
PciSI GCTCTTC 1 cut(s) 211
PctI GAATGC 2 cut(s) 206, 258
PdmI GAANNNNTTC 1 cut(s) 50
PfeI GAWTC 1 cut(s) 785
PkrI GCNGC 1 cut(s) 356
PpuMI RGGWCCY 1 cut(s) 17
Psp5II RGGWCCY 1 cut(s) 17
Psp6I CCWGG 2 cut(s) 121, 713
PspGI CCWGG 2 cut(s) 121, 713
PspN4I GGNNCC 2 cut(s) 18, 33
PspPI GGNCC 3 cut(s) 17, 31, 596
PspPPI RGGWCCY 1 cut(s) 17
PsrI GAACNNNNNNTAC 2 cut(s) 222, 254
PsuI RGATCY 1 cut(s) 94
RsaI GTAC 4 cut(s) 24, 74, 231, 614
RsaNI GTAC 4 cut(s) 23, 73, 230, 613
SapI GCTCTTC 1 cut(s) 211
SaqAI TTAA 2 cut(s) 579, 790
SatI GCNGC 1 cut(s) 355
Sau3AI GATC 6 cut(s) 94, 334, 388, 454, 661, 765
Sau96I GGNCC 3 cut(s) 17, 31, 596
ScrFI CCNGG 2 cut(s) 123, 715
SduI GDGCHC 1 cut(s) 179
SetI ASST 6 cut(s) 22, 184, 297, 327, 537, 636
SfaNI GCATC 2 cut(s) 70, 119
SinI GGWCC 1 cut(s) 17
SphI GCATGC 1 cut(s) 258
Sse9I AATT 8 cut(s) 78, 151, 193, 364, 420, 539, 553, 580
SsiI CCGC 3 cut(s) 227, 354, 490
SspI AATATT 1 cut(s) 384
StyD4I CCNGG 2 cut(s) 121, 713
TaaI ACNGT 2 cut(s) 234, 411
TaqI TCGA 3 cut(s) 211, 609, 682
TasI AATT 8 cut(s) 78, 151, 193, 364, 420, 539, 553, 580
TatI WGTACW 3 cut(s) 22, 72, 612
TauI GCSGC 1 cut(s) 357
TfiI GAWTC 1 cut(s) 785
Tru1I TTAA 2 cut(s) 579, 790
Tru9I TTAA 2 cut(s) 579, 790
TscAI CASTG 2 cut(s) 398, 416
TseFI GTSAC 1 cut(s) 405
Tsp45I GTSAC 1 cut(s) 405
TspDTI ATGAA 1 cut(s) 522
TspRI CASTG 2 cut(s) 398, 416
VpaK11BI GGWCC 1 cut(s) 17
XapI RAATTY 3 cut(s) 151, 539, 553
XceI RCATGY 1 cut(s) 258
XmiI GTMKAC 1 cut(s) 587
XmnI GAANNNNTTC 1 cut(s) 50
Zsp2I ATGCAT 2 cut(s) 112, 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.