RLG00000006654

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001447
Physical Location & Seq
Forward (+)
9363853 .. 9364920
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006654

Sequence Viewer

Length: 783 bp
ATGTTAGTGCCAACAAATGTAAACGCCTCAACATCCTACATGAGCCATGTTCGTATAGGACTCAAAGCATATCTGGAGCCGCCTAATGTTATGCACGACATGAATGATGTAGAATTGCCATGGAGAGCTTCGACGGCTCCTCGCATTTCTGAATATCCATTCCATCGAGTTCCAAAAGTTGCTTTCATGTTCTTGACAGAGGGACCTGTATATTTGGCCCCATTGTGGGAGAAGTTCTTCAAAGGGCATCAAGGCTTGTACTCAATTTATGTTCACTCAGATCCATCTTACAATGCATCATCACACCCAGAAAGTCCTGTTTTTCAAGGCCGAAGAATTCCAAGTCAGCGCTTCGTTCTCCTCTCCGAAGCATGCATTCCCCTATACAACTTCTCCACTATCTACTCTTACCTCCTAAACTCCCAAGAAACTTTTGTGGAGGTCTATGATGATCCAAGTTCAGTTGGACGCGGCAGATATAATTCAAGTTACTACCCAATAATCACATTGGATCAGTGGAGGAAAGGGTCACAGTGGGTTGAAATTGATAGAGACATTGCCATTGAAGTTGTCTCGGATCGGACATACTTACCAGCCTTCCGGTTGGGCAACCGCTCTTGTTTTGCAGATGAACATTACTTGCCAACATTTGTGAACCTAAAATTTTCGGCAAAGAATTCAAACAGGAGTGTCACCTGGTTTGATTGGAAGTTCCGGCCTTCTGCTTTGGATAGCCTTCTCAGGCTTGCCCCCAAGATCATGCACTTCGACACAGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

261

Amino Acids

30.12

Weight (kDa)

7.09

Isoelectric Point (pI)

52.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 60 - 116 1.2e-12 Core-2/I-Branching enzyme
Branch PF02485 116 - 237 4.3e-37 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 615
AccII CGCG 1 cut(s) 471
AciI CCGC 3 cut(s) 80, 471, 613
AclWI GGATC 4 cut(s) 275, 446, 519, 585
AcsI RAATTY 3 cut(s) 336, 662, 676
AfaI GTAC 1 cut(s) 260
AfeI AGCGCT 1 cut(s) 350
AfiI CCNNNNNNNGG 2 cut(s) 225, 226
AgsI TTSAA 6 cut(s) 241, 326, 486, 542, 566, 681
AjnI CCWGG 1 cut(s) 695
AluBI AGCT 1 cut(s) 128
AluI AGCT 1 cut(s) 128
Alw26I GTCTC 2 cut(s) 546, 577
AlwI GGATC 4 cut(s) 275, 446, 519, 585
Aor51HI AGCGCT 1 cut(s) 350
AoxI GGCC 3 cut(s) 216, 328, 716
ApoI RAATTY 3 cut(s) 336, 662, 676
Asp700I GAANNNNTTC 1 cut(s) 236
AspLEI GCGC 1 cut(s) 351
AspS9I GGNCC 2 cut(s) 203, 217
AsuHPI GGTGA 1 cut(s) 685
AvaII GGWCC 1 cut(s) 203
BccI CCATC 2 cut(s) 171, 292
BceAI ACGGC 1 cut(s) 150
BciT130I CCWGG 1 cut(s) 697
BcoDI GTCTC 2 cut(s) 546, 577
BfoI RGCGCY 1 cut(s) 352
BisI GCNGC 2 cut(s) 80, 472
BlsI GCNGC 2 cut(s) 81, 473
Bme1390I CCNGG 1 cut(s) 697
Bme18I GGWCC 1 cut(s) 203
BmgT120I GGNCC 2 cut(s) 203, 217
BmiI GGNNCC 4 cut(s) 78, 138, 204, 219
BmrFI CCNGG 1 cut(s) 697
BmsI GCATC 2 cut(s) 256, 305
BpmI CTGGAG 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 119
BsaWI WCCGGW 1 cut(s) 600
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 226
Bse3DI GCAATG 1 cut(s) 555
BseBI CCWGG 1 cut(s) 697
BseDI CCNNGG 1 cut(s) 119
BseGI GGATG 1 cut(s) 32
BseLI CCNNNNNNNGG 2 cut(s) 225, 226
BseMI GCAATG 1 cut(s) 555
BseMII CTCAG 2 cut(s) 291, 754
BseRI GAGGAG 2 cut(s) 129, 350
Bsh1236I CGCG 1 cut(s) 471
BshFI GGCC 3 cut(s) 218, 330, 718
BsiSI CCGG 2 cut(s) 601, 715
BslFI GGGAC 1 cut(s) 216
BslI CCNNNNNNNGG 2 cut(s) 225, 226
BsmAI GTCTC 2 cut(s) 546, 577
BsmFI GGGAC 1 cut(s) 216
BsmI GAATGC 1 cut(s) 375
BsnI GGCC 3 cut(s) 218, 330, 718
Bsp143I GATC 5 cut(s) 280, 451, 511, 577, 756
Bsp19I CCATGG 1 cut(s) 119
BspACI CCGC 3 cut(s) 80, 471, 613
BspANI GGCC 3 cut(s) 218, 330, 718
BspCNI CTCAG 2 cut(s) 290, 753
BspFNI CGCG 1 cut(s) 471
BspLI GGNNCC 4 cut(s) 78, 138, 204, 219
BspPI GGATC 4 cut(s) 275, 446, 519, 585
BsrBI CCGCTC 1 cut(s) 615
BsrDI GCAATG 1 cut(s) 555
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 5 cut(s) 280, 451, 511, 577, 756
BssT1I CCWWGG 1 cut(s) 119
Bst2UI CCWGG 1 cut(s) 697
Bst4CI ACNGT 1 cut(s) 534
BstC8I GCNNGC 2 cut(s) 373, 747
BstDEI CTNAG 2 cut(s) 277, 740
BstDSI CCRYGG 1 cut(s) 119
BstF5I GGATG 1 cut(s) 32
BstFNI CGCG 1 cut(s) 471
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 1 cut(s) 351
BstKTI GATC 5 cut(s) 283, 454, 514, 580, 759
BstMAI GTCTC 2 cut(s) 546, 577
BstMBI GATC 5 cut(s) 280, 451, 511, 577, 756
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 1 cut(s) 697
BstNSI RCATGY 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 695
BstUI CGCG 1 cut(s) 471
BstX2I RGATCY 1 cut(s) 280
BstYI RGATCY 1 cut(s) 280
BsuRI GGCC 3 cut(s) 218, 330, 718
BtgI CCRYGG 1 cut(s) 119
BtsCI GGATG 1 cut(s) 32
BtsIMutI CAGTG 2 cut(s) 521, 539
Cac8I GCNNGC 2 cut(s) 373, 747
CfoI GCGC 1 cut(s) 351
Cfr13I GGNCC 2 cut(s) 203, 217
CseI GACGC 1 cut(s) 477
CsiI ACCWGGT 1 cut(s) 695
Csp6I GTAC 1 cut(s) 259
CviAII CATG 7 cut(s) 40, 47, 100, 120, 187, 372, 760
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 2 cut(s) 277, 740
DpnI GATC 5 cut(s) 282, 453, 513, 579, 758
DpnII GATC 5 cut(s) 280, 451, 511, 577, 756
Eco130I CCWWGG 1 cut(s) 119
Eco47I GGWCC 1 cut(s) 203
Eco47III AGCGCT 1 cut(s) 350
EcoO109I RGGNCCY 1 cut(s) 203
EcoRI GAATTC 2 cut(s) 336, 676
EcoRII CCWGG 1 cut(s) 695
EcoT14I CCWWGG 1 cut(s) 119
EcoT22I ATGCAT 2 cut(s) 298, 377
ErhI CCWWGG 1 cut(s) 119
FaeI CATG 7 cut(s) 43, 50, 103, 123, 190, 375, 763
FaqI GGGAC 1 cut(s) 216
FatI CATG 7 cut(s) 39, 46, 99, 119, 186, 371, 759
Fnu4HI GCNGC 2 cut(s) 80, 472
FokI GGATG 1 cut(s) 19
Fsp4HI GCNGC 2 cut(s) 80, 472
GlaI GCGC 1 cut(s) 350
GluI GCNGC 2 cut(s) 80, 472
GsuI CTGGAG 1 cut(s) 95
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 3 cut(s) 218, 330, 718
HapII CCGG 2 cut(s) 601, 715
HgaI GACGC 1 cut(s) 477
HhaI GCGC 1 cut(s) 351
Hin1II CATG 7 cut(s) 43, 50, 103, 123, 190, 375, 763
Hin6I GCGC 1 cut(s) 349
HinP1I GCGC 1 cut(s) 349
HinfI GANTC 2 cut(s) 60, 776
HpaII CCGG 2 cut(s) 601, 715
HphI GGTGA 1 cut(s) 685
Hpy166II GTNNAC 3 cut(s) 22, 274, 655
Hpy188I TCNGA 5 cut(s) 151, 280, 367, 577, 582
Hpy188III TCNNGA 2 cut(s) 74, 193
Hpy8I GTNNAC 3 cut(s) 22, 274, 655
Hpy99I CGWCG 1 cut(s) 136
HpyAV CCTTC 3 cut(s) 607, 729, 746
HpyCH4III ACNGT 1 cut(s) 534
HpyCH4V TGCA 5 cut(s) 94, 296, 375, 626, 763
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 2 cut(s) 277, 740
Hsp92II CATG 7 cut(s) 43, 50, 103, 123, 190, 375, 763
HspAI GCGC 1 cut(s) 349
Kzo9I GATC 5 cut(s) 280, 451, 511, 577, 756
LmnI GCTCC 2 cut(s) 76, 142
LweI GCATC 2 cut(s) 256, 305
MabI ACCWGGT 1 cut(s) 695
MaeIII GTNAC 3 cut(s) 488, 528, 691
MalI GATC 5 cut(s) 282, 453, 513, 579, 758
MbiI CCGCTC 1 cut(s) 615
MboI GATC 5 cut(s) 280, 451, 511, 577, 756
MboII GAAGA 2 cut(s) 229, 345
MflI RGATCY 1 cut(s) 280
MluCI AATT 7 cut(s) 113, 264, 336, 481, 543, 662, 676
MlyI GAGTC 1 cut(s) 54
MmeI TCCRAC 1 cut(s) 445
MnlI CCTC 7 cut(s) 37, 150, 193, 371, 422, 433, 513
Mph1103I ATGCAT 2 cut(s) 298, 377
MroXI GAANNNNTTC 1 cut(s) 236
MseI TTAA 1 cut(s) 781
MspI CCGG 2 cut(s) 601, 715
MspR9I CCNGG 1 cut(s) 697
Mva1269I GAATGC 1 cut(s) 375
MvaI CCWGG 1 cut(s) 697
MvnI CGCG 1 cut(s) 471
MwoI GCNNNNNNNGC 1 cut(s) 134
NcoI CCATGG 1 cut(s) 119
NdeII GATC 5 cut(s) 280, 451, 511, 577, 756
NlaIII CATG 7 cut(s) 43, 50, 103, 123, 190, 375, 763
NlaIV GGNNCC 4 cut(s) 78, 138, 204, 219
NmuCI GTSAC 2 cut(s) 528, 691
NsiI ATGCAT 2 cut(s) 298, 377
NspI RCATGY 1 cut(s) 375
PaeI GCATGC 1 cut(s) 375
PctI GAATGC 1 cut(s) 375
PdmI GAANNNNTTC 1 cut(s) 236
PfeI GAWTC 1 cut(s) 776
PkrI GCNGC 2 cut(s) 81, 473
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
PpuMI RGGWCCY 1 cut(s) 203
Psp5II RGGWCCY 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 695
PspGI CCWGG 1 cut(s) 695
PspN4I GGNNCC 4 cut(s) 78, 138, 204, 219
PspPI GGNCC 2 cut(s) 203, 217
PspPPI RGGWCCY 1 cut(s) 203
PsuI RGATCY 1 cut(s) 280
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
SaqAI TTAA 1 cut(s) 781
SatI GCNGC 2 cut(s) 80, 472
Sau3AI GATC 5 cut(s) 280, 451, 511, 577, 756
Sau96I GGNCC 2 cut(s) 203, 217
SchI GAGTC 1 cut(s) 54
ScrFI CCNGG 1 cut(s) 697
SetI ASST 6 cut(s) 130, 208, 414, 444, 660, 698
SexAI ACCWGGT 1 cut(s) 695
SfaNI GCATC 2 cut(s) 256, 305
SinI GGWCC 1 cut(s) 203
SphI GCATGC 1 cut(s) 375
Sse9I AATT 7 cut(s) 113, 264, 336, 481, 543, 662, 676
SsiI CCGC 3 cut(s) 80, 471, 613
StyD4I CCNGG 1 cut(s) 695
StyI CCWWGG 1 cut(s) 119
TaaI ACNGT 1 cut(s) 534
TaqI TCGA 3 cut(s) 131, 166, 768
TasI AATT 7 cut(s) 113, 264, 336, 481, 543, 662, 676
TatI WGTACW 1 cut(s) 258
TauI GCSGC 2 cut(s) 82, 474
TfiI GAWTC 1 cut(s) 776
Tru1I TTAA 1 cut(s) 781
Tru9I TTAA 1 cut(s) 781
TscAI CASTG 2 cut(s) 521, 539
TseFI GTSAC 2 cut(s) 528, 691
Tsp45I GTSAC 2 cut(s) 528, 691
TspDTI ATGAA 3 cut(s) 116, 175, 645
TspRI CASTG 2 cut(s) 521, 539
VpaK11BI GGWCC 1 cut(s) 203
XapI RAATTY 3 cut(s) 336, 662, 676
XceI RCATGY 1 cut(s) 375
XmnI GAANNNNTTC 1 cut(s) 236
Zsp2I ATGCAT 2 cut(s) 298, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.