pycom13g03350

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0001447
Physical Location & Seq
Reverse (-)
2200004 .. 2201849
1846 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g03350.2

Sequence Viewer

Length: 1203 bp
ATGAAGGGACCAAATCAAAACCCTTTAAACTTCTTCTCAAAGCTCTTCAATGTCCAGCTCCACTTGCTCAATCTCCTCTTCTACTTCCTCGTCTTCGGCGGCGGCCTGACCCTCGGAATGGTGCTCAGCTTCTACATGAAAGACTTTTTCGTCAGTCTGCAATTAGCACAGTTCTCCTTCTCAACCTCAACATCTGCATCCAATCCGCACAACCTCATGCCGAACGTTAAAGCAGTTGAACCCAACACGAGTCACCGTCGTCCTCACGTAGGATTGAAGGAATATCTAAAGCCGCCGGAGGTTTTCCACGACATGGACGATGTGGAGTTGCTGTGGAGAGCTTCGACGAGTCCTCGGATTTCCGAGTATCCGTTTCCCCGTGTTCCCAAGGTTGCTTTTATGTTCTTGGTAAAGGGTCCTGTTTTTTTGGCCCCGTTGTGGGAGAAGTTCTTCAAAGGACATAAAGGATTGTACTCGATTTACGTGCATTCGAATCCATCTTACAATGGATCGCACCCGGAAAGTCCAGTTTTTCATGGCAGAAGAATTCCCAGCCAGGAAGTAGAATGGGGAAAGGTTAACATGATTGAGGCCGAGCGCCGCCTATTGGCGAATGCTCTTCTCGACATCTCAAACCAACGGTTCATTCTGCTCTCAGAGTCATGCATTCCCCTCCACAACTTCCCCACTGTCTACAATTACCTCATTCGCTCCAATGAAACTTTTGTGGGAGTCTATGATGATCCAAGCTCGGTTGGGCGTGGCAGGTACAGTTCTCACATGTATCCACAAATCTCGTTGAGTCAATGGCGAAAAGGGTCGCAATGGTTTGAAATCGACAGAAGCCTTGCCATTGAAGTTGTCTCGGATAAAAAATACTTCCCGTTGTTCCAAACTTACTGCAGGGGCTCATGCTACGCAGATGAGCATTACTTGCCAACATATGTGAACATAAAATTTGCACAGAAGAATTCAAATAGGAGTTTGACCTGGGTTGACTGGTCGAGGGGCGGTCCACATCCAGCCAAGTTTACTAGAACGGATGTGACGGTCGAGGTTTTGAATAGCTTGAGGAATGGGACTGCATGTATATATAATGGAAGAGTTTCCAATGTTTGTTTCTTGTTTGCTAGGAAGTTTGGGCCTGGTACACTGGAGAGACTGCTGAGGTTTGTGCCGAAGCTCATGCGATTCAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

401

Amino Acids

46.17

Weight (kDa)

9.6

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 149
Acc36I ACCTGC 1 cut(s) 756
AccB7I CCANNNNNTGG 1 cut(s) 313
AccI GTMKAC 1 cut(s) 693
AciI CCGC 6 cut(s) 99, 102, 206, 293, 601, 1011
AclI AACGTT 1 cut(s) 225
AclWI GGATC 2 cut(s) 517, 737
AcsI RAATTY 3 cut(s) 546, 956, 970
AfaI GTAC 3 cut(s) 473, 770, 1150
AfiI CCNNNNNNNGG 6 cut(s) 118, 269, 313, 438, 439, 607
AflIII ACRYGT 1 cut(s) 780
AgsI TTSAA 9 cut(s) 49, 239, 277, 454, 833, 857, 975, 1063, 1195
AjnI CCWGG 3 cut(s) 555, 989, 1144
AjuI GAANNNNNNNTTGG 4 cut(s) 630, 662, 1103, 1135
AluBI AGCT 7 cut(s) 43, 58, 129, 341, 750, 1068, 1183
AluI AGCT 7 cut(s) 43, 58, 129, 341, 750, 1068, 1183
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 2 cut(s) 868, 1153
AlwI GGATC 2 cut(s) 517, 737
AoxI GGCC 4 cut(s) 103, 429, 591, 1142
ApoI RAATTY 3 cut(s) 546, 956, 970
Asp700I GAANNNNTTC 2 cut(s) 449, 1105
AspLEI GCGC 1 cut(s) 600
AspS9I GGNCC 5 cut(s) 8, 416, 430, 1013, 1142
AsuC2I CCSGG 1 cut(s) 518
AsuHPI GGTGA 1 cut(s) 245
AsuII TTCGAA 1 cut(s) 491
AvaII GGWCC 3 cut(s) 8, 416, 1013
BanII GRGCYC 1 cut(s) 911
BauI CACGAG 1 cut(s) 247
BbsI GAAGAC 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 126
BbvCI CCTCAGC 1 cut(s) 1166
BccI CCATC 1 cut(s) 505
BcgI CGANNNNNNTGC 4 cut(s) 466, 500, 1168, 1202
BciT130I CCWGG 3 cut(s) 557, 991, 1146
BciVI GTATCC 2 cut(s) 378, 795
BcnI CCSGG 1 cut(s) 518
BcoDI GTCTC 2 cut(s) 868, 1153
BfaI CTAG 2 cut(s) 1035, 1131
BfmI CTRYAG 1 cut(s) 901
BfoI RGCGCY 1 cut(s) 601
BfuAI ACCTGC 1 cut(s) 756
BfuI GTATCC 2 cut(s) 378, 795
BisI GCNGC 4 cut(s) 100, 103, 293, 601
BlpI GCTNAGC 1 cut(s) 125
BlsI GCNGC 4 cut(s) 101, 104, 294, 602
Bme1390I CCNGG 4 cut(s) 518, 557, 991, 1146
Bme18I GGWCC 3 cut(s) 8, 416, 1013
BmgT120I GGNCC 5 cut(s) 8, 416, 430, 1013, 1142
BmiI GGNNCC 3 cut(s) 9, 417, 432
BmrFI CCNGG 4 cut(s) 518, 557, 991, 1146
BmsI GCATC 1 cut(s) 206
BpiI GAAGAC 1 cut(s) 85
BpmI CTGGAG 1 cut(s) 1175
Bpu10I CCTNAGC 1 cut(s) 1166
Bpu1102I GCTNAGC 1 cut(s) 125
Bpu14I TTCGAA 1 cut(s) 491
BpuEI CTTGAG 1 cut(s) 1090
BpuMI CCSGG 1 cut(s) 518
BsaAI YACGTR 2 cut(s) 268, 484
BsaJI CCNNGG 4 cut(s) 112, 353, 387, 990
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
Bsc4I CCNNNNNNNGG 6 cut(s) 118, 269, 313, 438, 439, 607
Bse1I ACTGG 3 cut(s) 527, 1004, 1158
Bse3DI GCAATG 1 cut(s) 830
BseBI CCWGG 3 cut(s) 557, 991, 1146
BseDI CCNNGG 4 cut(s) 112, 353, 387, 990
BseGI GGATG 3 cut(s) 197, 1018, 1048
BseLI CCNNNNNNNGG 6 cut(s) 118, 269, 313, 438, 439, 607
BseMI GCAATG 1 cut(s) 830
BseMII CTCAG 3 cut(s) 139, 669, 1157
BseNI ACTGG 3 cut(s) 527, 1004, 1158
BseRI GAGGAG 1 cut(s) 65
BseYI CCCAGC 1 cut(s) 551
Bsh1285I CGRYCG 1 cut(s) 1053
BshFI GGCC 4 cut(s) 105, 431, 593, 1144
BsiEI CGRYCG 1 cut(s) 1053
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 2 cut(s) 296, 518
BslFI GGGAC 2 cut(s) 21, 1093
BslI CCNNNNNNNGG 6 cut(s) 118, 269, 313, 438, 439, 607
BsmAI GTCTC 2 cut(s) 868, 1153
BsmFI GGGAC 2 cut(s) 21, 1093
BsmI GAATGC 3 cut(s) 487, 619, 666
BsnI GGCC 4 cut(s) 105, 431, 593, 1144
Bsp119I TTCGAA 1 cut(s) 491
Bsp1286I GDGCHC 2 cut(s) 126, 911
Bsp143I GATC 2 cut(s) 509, 742
Bsp1720I GCTNAGC 1 cut(s) 125
BspACI CCGC 6 cut(s) 99, 102, 206, 293, 601, 1011
BspANI GGCC 4 cut(s) 105, 431, 593, 1144
BspCNI CTCAG 3 cut(s) 138, 668, 1158
BspLI GGNNCC 3 cut(s) 9, 417, 432
BspMAI CTGCAG 1 cut(s) 905
BspMI ACCTGC 1 cut(s) 756
BspPI GGATC 2 cut(s) 517, 737
BspQI GCTCTTC 2 cut(s) 50, 624
BspT104I TTCGAA 1 cut(s) 491
BsrDI GCAATG 1 cut(s) 830
BsrI ACTGG 3 cut(s) 527, 1004, 1158
BssECI CCNNGG 4 cut(s) 112, 353, 387, 990
BssMI GATC 2 cut(s) 509, 742
BssSI CACGAG 1 cut(s) 247
BssT1I CCWWGG 1 cut(s) 387
Bst2BI CACGAG 1 cut(s) 247
Bst2UI CCWGG 3 cut(s) 557, 991, 1146
Bst4CI ACNGT 6 cut(s) 171, 257, 642, 691, 773, 1051
Bst6I CTCTTC 4 cut(s) 50, 83, 624, 1096
BstAPI GCANNNNNTGC 1 cut(s) 934
BstBAI YACGTR 2 cut(s) 268, 484
BstBI TTCGAA 1 cut(s) 491
BstDEI CTNAG 3 cut(s) 125, 655, 1166
BstENI CCTNNNNNAGG 1 cut(s) 267
BstF5I GGATG 3 cut(s) 197, 1018, 1048
BstH2I RGCGCY 1 cut(s) 601
BstHHI GCGC 1 cut(s) 600
BstKTI GATC 2 cut(s) 512, 745
BstMAI GTCTC 2 cut(s) 868, 1153
BstMBI GATC 2 cut(s) 509, 742
BstMCI CGRYCG 1 cut(s) 1053
BstMWI GCNNNNNNNGC 2 cut(s) 64, 934
BstNI CCWGG 3 cut(s) 557, 991, 1146
BstNSI RCATGY 2 cut(s) 784, 1089
BstSCI CCNGG 4 cut(s) 516, 555, 989, 1144
BstSFI CTRYAG 1 cut(s) 901
BstV2I GAAGAC 1 cut(s) 85
BsuI GTATCC 2 cut(s) 378, 795
BsuRI GGCC 4 cut(s) 105, 431, 593, 1144
BtsCI GGATG 3 cut(s) 197, 1018, 1048
BtsIMutI CAGTG 2 cut(s) 687, 1151
BveI ACCTGC 1 cut(s) 756
CfoI GCGC 1 cut(s) 600
Cfr13I GGNCC 5 cut(s) 8, 416, 430, 1013, 1142
Csp6I GTAC 3 cut(s) 472, 769, 1149
CviQI GTAC 3 cut(s) 472, 769, 1149
DdeI CTNAG 3 cut(s) 125, 655, 1166
DpnI GATC 2 cut(s) 511, 744
DpnII GATC 2 cut(s) 509, 742
DraI TTTAAA 1 cut(s) 27
DrdI GACNNNNNNGTC 1 cut(s) 149
DseDI GACNNNNNNGTC 1 cut(s) 149
Eam1104I CTCTTC 4 cut(s) 50, 83, 624, 1096
EarI CTCTTC 4 cut(s) 50, 83, 624, 1096
Eco130I CCWWGG 1 cut(s) 387
Eco24I GRGCYC 1 cut(s) 911
Eco47I GGWCC 3 cut(s) 8, 416, 1013
EcoNI CCTNNNNNAGG 1 cut(s) 267
EcoO109I RGGNCCY 1 cut(s) 416
EcoRI GAATTC 2 cut(s) 546, 970
EcoRII CCWGG 3 cut(s) 555, 989, 1144
EcoT14I CCWWGG 1 cut(s) 387
EcoT22I ATGCAT 1 cut(s) 668
EcoT38I GRGCYC 1 cut(s) 911
ErhI CCWWGG 1 cut(s) 387
FaqI GGGAC 2 cut(s) 21, 1093
FauNDI CATATG 1 cut(s) 943
FblI GTMKAC 1 cut(s) 693
Fnu4HI GCNGC 4 cut(s) 100, 103, 293, 601
FokI GGATG 3 cut(s) 184, 1005, 1055
FriOI GRGCYC 1 cut(s) 911
Fsp4HI GCNGC 4 cut(s) 100, 103, 293, 601
FspBI CTAG 2 cut(s) 1035, 1131
GlaI GCGC 1 cut(s) 599
GluI GCNGC 4 cut(s) 100, 103, 293, 601
GsaI CCCAGC 1 cut(s) 555
GsuI CTGGAG 1 cut(s) 1175
HaeII RGCGCY 1 cut(s) 601
HaeIII GGCC 4 cut(s) 105, 431, 593, 1144
HapII CCGG 2 cut(s) 296, 518
HhaI GCGC 1 cut(s) 600
Hin6I GCGC 1 cut(s) 598
HinP1I GCGC 1 cut(s) 598
HincII GTYRAC 2 cut(s) 580, 997
HindII GTYRAC 2 cut(s) 580, 997
HinfI GANTC 7 cut(s) 250, 349, 493, 659, 732, 802, 1191
HpaI GTTAAC 1 cut(s) 580
HpaII CCGG 2 cut(s) 296, 518
HphI GGTGA 1 cut(s) 245
Hpy166II GTNNAC 7 cut(s) 580, 694, 949, 997, 1016, 1032, 1151
Hpy188I TCNGA 5 cut(s) 116, 357, 364, 658, 868
Hpy188III TCNNGA 1 cut(s) 623
Hpy8I GTNNAC 7 cut(s) 580, 694, 949, 997, 1016, 1032, 1151
Hpy99I CGWCG 2 cut(s) 261, 349
HpyAV CCTTC 2 cut(s) 187, 271
HpyCH4III ACNGT 6 cut(s) 171, 257, 642, 691, 773, 1051
HpyCH4IV ACGT 3 cut(s) 225, 267, 483
HpyCH4V TGCA 7 cut(s) 160, 197, 487, 666, 903, 962, 1085
HpyF10VI GCNNNNNNNGC 2 cut(s) 64, 934
HpyF3I CTNAG 3 cut(s) 125, 655, 1166
HpySE526I ACGT 3 cut(s) 225, 267, 483
HspAI GCGC 1 cut(s) 598
KspAI GTTAAC 1 cut(s) 580
Kzo9I GATC 2 cut(s) 509, 742
LguI GCTCTTC 2 cut(s) 50, 624
LmnI GCTCC 2 cut(s) 63, 716
LweI GCATC 1 cut(s) 206
MaeI CTAG 2 cut(s) 1035, 1131
MaeII ACGT 3 cut(s) 225, 267, 483
MaeIII GTNAC 2 cut(s) 251, 1045
MalI GATC 2 cut(s) 511, 744
MboI GATC 2 cut(s) 509, 742
MboII GAAGA 9 cut(s) 25, 37, 70, 85, 442, 555, 611, 979, 1113
MhlI GDGCHC 2 cut(s) 126, 911
MluCI AATT 5 cut(s) 161, 546, 697, 956, 970
MlyI GAGTC 5 cut(s) 259, 358, 668, 741, 811
Mph1103I ATGCAT 1 cut(s) 668
MroXI GAANNNNTTC 2 cut(s) 449, 1105
MseI TTAA 3 cut(s) 26, 228, 579
MspI CCGG 2 cut(s) 296, 518
MspR9I CCNGG 4 cut(s) 518, 557, 991, 1146
Mva1269I GAATGC 3 cut(s) 487, 619, 666
MvaI CCWGG 3 cut(s) 557, 991, 1146
MwoI GCNNNNNNNGC 2 cut(s) 64, 934
NciI CCSGG 1 cut(s) 518
NdeI CATATG 1 cut(s) 943
NdeII GATC 2 cut(s) 509, 742
NlaIV GGNNCC 3 cut(s) 9, 417, 432
NmeAIII GCCGAG 1 cut(s) 619
NmuCI GTSAC 2 cut(s) 251, 1045
NsiI ATGCAT 1 cut(s) 668
NspI RCATGY 2 cut(s) 784, 1089
NspV TTCGAA 1 cut(s) 491
PciI ACATGT 1 cut(s) 780
PciSI GCTCTTC 2 cut(s) 50, 624
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PctI GAATGC 3 cut(s) 487, 619, 666
PdmI GAANNNNTTC 2 cut(s) 449, 1105
PfeI GAWTC 2 cut(s) 493, 1191
PflMI CCANNNNNTGG 1 cut(s) 313
PkrI GCNGC 4 cut(s) 101, 104, 294, 602
PleI GAGTC 5 cut(s) 258, 357, 667, 740, 810
PpsI GAGTC 5 cut(s) 258, 357, 667, 740, 810
Ppu21I YACGTR 2 cut(s) 268, 484
PpuMI RGGWCCY 1 cut(s) 416
PscI ACATGT 1 cut(s) 780
Psp1406I AACGTT 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 416
Psp6I CCWGG 3 cut(s) 555, 989, 1144
PspFI CCCAGC 1 cut(s) 551
PspGI CCWGG 3 cut(s) 555, 989, 1144
PspN4I GGNNCC 3 cut(s) 9, 417, 432
PspPI GGNCC 5 cut(s) 8, 416, 430, 1013, 1142
PspPPI RGGWCCY 1 cut(s) 416
PstI CTGCAG 1 cut(s) 905
RsaI GTAC 3 cut(s) 473, 770, 1150
RsaNI GTAC 3 cut(s) 472, 769, 1149
SapI GCTCTTC 2 cut(s) 50, 624
SaqAI TTAA 3 cut(s) 26, 228, 579
SatI GCNGC 4 cut(s) 100, 103, 293, 601
Sau3AI GATC 2 cut(s) 509, 742
Sau96I GGNCC 5 cut(s) 8, 416, 430, 1013, 1142
SchI GAGTC 5 cut(s) 259, 358, 668, 741, 811
ScrFI CCNGG 4 cut(s) 518, 557, 991, 1146
SduI GDGCHC 2 cut(s) 126, 911
SfaNI GCATC 1 cut(s) 206
SfcI CTRYAG 1 cut(s) 901
SfuI TTCGAA 1 cut(s) 491
SinI GGWCC 3 cut(s) 8, 416, 1013
SmlI CTYRAG 1 cut(s) 1069
SmoI CTYRAG 1 cut(s) 1069
Sse9I AATT 5 cut(s) 161, 546, 697, 956, 970
SsiI CCGC 6 cut(s) 99, 102, 206, 293, 601, 1011
SspMI CTAG 2 cut(s) 1035, 1131
StyD4I CCNGG 4 cut(s) 516, 555, 989, 1144
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 6 cut(s) 171, 257, 642, 691, 773, 1051
TaiI ACGT 3 cut(s) 228, 270, 486
TaqI TCGA 7 cut(s) 344, 476, 491, 624, 837, 1004, 1053
TasI AATT 5 cut(s) 161, 546, 697, 956, 970
TatI WGTACW 1 cut(s) 471
TauI GCSGC 4 cut(s) 102, 105, 295, 603
TfiI GAWTC 2 cut(s) 493, 1191
Tru1I TTAA 3 cut(s) 26, 228, 579
Tru9I TTAA 3 cut(s) 26, 228, 579
TscAI CASTG 2 cut(s) 694, 1158
TseFI GTSAC 2 cut(s) 251, 1045
Tsp45I GTSAC 2 cut(s) 251, 1045
TspDTI ATGAA 5 cut(s) 17, 152, 524, 634, 732
TspGWI ACGGA 2 cut(s) 360, 1055
TspRI CASTG 2 cut(s) 694, 1158
Van91I CCANNNNNTGG 1 cut(s) 313
VpaK11BI GGWCC 3 cut(s) 8, 416, 1013
XagI CCTNNNNNAGG 1 cut(s) 267
XapI RAATTY 3 cut(s) 546, 956, 970
XceI RCATGY 2 cut(s) 784, 1089
XmiI GTMKAC 1 cut(s) 693
XmnI GAANNNNTTC 2 cut(s) 449, 1105
XspI CTAG 2 cut(s) 1035, 1131
Zsp2I ATGCAT 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.