Rw4G029030

Core-2/I-Branching enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001447
Physical Location & Seq
Reverse (-)
55717121 .. 55718888
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029030.1

Sequence Viewer

Length: 1254 bp
ATGAAGGATCATAATCAAAACCCTTCAAACTTCTTCACAAAACTAATCAATGGCCAAGGCCACCTCCTTAATCTCCTCTCTTATTTCCTCACCTTTGGTGGTGGTTTAGTTCTCGGAATCGTACTTGCCTCTTCTTATCTCAAAGACTCTTCCTTTAGTCTACAAATAGCCCAACTATCATTCTCCTCTTCTTCTTCTTCTTCATCATCTCCGTCCAATAGTTCCAATATTTCACATATGACAATTAGTCCAAATGACACAGTCACGATGTCTAACATGACCGAAGTTCATATACGAGAATTAGAGGCATTTCTAAAGCCGCCTAAGGTTCGGCACGACATGGACGATGAGGAATTGTTCTGGAGAGCTTCCATGGCCCCTCGGATACCCGAATATCCGTTCCACCGAGTTCCGAAAGTTGCTTTCATGTTCTTGACAAGGGGACCTGTTCATTTGGCTCCATTGTGGGAGAAGTTCTTTCAAGGGCACCAAGGCTTGTTCTCAATCTATGTCCACTCAGATCCGTCTTATAATGAATCGTCTCACCCCGAAAGCCCAGTCTTTCATGGCCGAAGAATTCCAAGTCAGAGAGCAGAATGGGGGCAAGTGAACATGATTGAAGCCGAGAGCCGCGTAATTGCGAATGCTCTTCTCGACATCTCAAACCAACGTTTTGTTCTGCTTTCAGAATCATGCATCCCCCTCTTCAATTTCTCCACGGTCTACTCTTACCTCATAAACACCAACAAGACTTTTGTGCAGGTCTATGATGATCCAAGCGGTGTTGGGCGTGGCCGTTATGATGAAAGCTACTACCCTCAAATCACATTGGACCAGTGGAGGAAAGGATCGCAGTGGGTTGAAATAGATAGAGACCTTGCCATTGAAGTTGTTTCAGATCAAAAATACTACACAACTTTCCAGAAACTCTGCAAAGGCTATTGCTTTGCAGATGAGCATTACTTGCCAACATTTATGAACATAAGATTTCCGAAAAAGAATTCAAACAGGACTGTGACCTGGGTTGATTGGTCTAGGGGCGGACCTCATCCTGTCGAGTACGCGAGCACAGATGTCACGGTTGAGCTTCTGAGTACTCTGAGGAAGGGTAACGGCTGGTGTGATTACAATGGAAGGGGCACAGATGTTTGTTTCTTGTTCGCCCGGAAGTTCTCGCCAAATTCTTTGGATAAGCTGCTGAGCATTGCACCACAGTTAATGCAATTCAACACAGACATTGATAAAGAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Branch

Protein Analysis

417

Amino Acids

47.7

Weight (kDa)

6.36

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Branch PF02485 140 - 367 3.7e-77 Core-2/I-Branching enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 531
Acc36I ACCTGC 1 cut(s) 751
AccB1I GGYRCC 1 cut(s) 486
AccI GTMKAC 2 cut(s) 160, 723
AccII CGCG 2 cut(s) 633, 1064
AciI CCGC 4 cut(s) 320, 631, 780, 1041
AclI AACGTT 1 cut(s) 670
AclWI GGATC 4 cut(s) 15, 515, 767, 856
AcoI YGGCCR 3 cut(s) 52, 568, 793
AcsI RAATTY 3 cut(s) 576, 1000, 1180
AfaI GTAC 4 cut(s) 123, 1061, 1096, 1250
AgsI TTSAA 8 cut(s) 27, 482, 620, 709, 863, 887, 1005, 1228
AhdI GACNNNNNGTC 1 cut(s) 246
AjnI CCWGG 1 cut(s) 1019
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AluBI AGCT 4 cut(s) 368, 810, 1087, 1195
AluI AGCT 4 cut(s) 368, 810, 1087, 1195
Alw21I GWGCWC 1 cut(s) 1070
Alw26I GTCTC 2 cut(s) 546, 867
AlwI GGATC 4 cut(s) 15, 515, 767, 856
AoxI GGCC 5 cut(s) 52, 58, 375, 568, 793
ApeKI GCWGC 1 cut(s) 1195
ApoI RAATTY 3 cut(s) 576, 1000, 1180
ArsI GACNNNNNNTTYG 2 cut(s) 543, 575
AspS9I GGNCC 4 cut(s) 376, 443, 832, 1043
AsuC2I CCSGG 1 cut(s) 1165
AsuHPI GGTGA 2 cut(s) 82, 536
AvaII GGWCC 3 cut(s) 443, 832, 1043
AxyI CCTNAGG 1 cut(s) 324
BaeGI GKGCMC 2 cut(s) 489, 1142
BalI TGGCCA 1 cut(s) 54
BanI GGYRCC 1 cut(s) 486
BarI GAAGNNNNNNTAC 2 cut(s) 276, 308
Bbv12I GWGCWC 1 cut(s) 1070
BbvI GCAGC 1 cut(s) 1182
BceAI ACGGC 2 cut(s) 780, 1129
BciT130I CCWGG 1 cut(s) 1021
BciVI GTATCC 1 cut(s) 378
BcnI CCSGG 1 cut(s) 1165
BcoDI GTCTC 2 cut(s) 546, 867
BfaI CTAG 2 cut(s) 1035, 1252
BfuAI ACCTGC 1 cut(s) 751
BfuI GTATCC 1 cut(s) 378
BisI GCNGC 3 cut(s) 320, 631, 1196
BlpI GCTNAGC 1 cut(s) 1199
BlsI GCNGC 3 cut(s) 321, 632, 1197
BmcAI AGTACT 2 cut(s) 1096, 1250
Bme1390I CCNGG 2 cut(s) 1021, 1165
Bme18I GGWCC 3 cut(s) 443, 832, 1043
BmeRI GACNNNNNGTC 1 cut(s) 246
BmgT120I GGNCC 4 cut(s) 376, 443, 832, 1043
BmiI GGNNCC 4 cut(s) 378, 444, 459, 488
BmrFI CCNGG 2 cut(s) 1021, 1165
BmrI ACTGGG 1 cut(s) 551
BmsI GCATC 1 cut(s) 705
BmuI ACTGGG 1 cut(s) 551
BpmI CTGGAG 1 cut(s) 382
Bpu1102I GCTNAGC 1 cut(s) 1199
BpuMI CCSGG 1 cut(s) 1165
BsaBI GATNNNNATC 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 867
BsaJI CCNNGG 6 cut(s) 55, 372, 380, 490, 717, 1020
Bse1I ACTGG 2 cut(s) 557, 835
Bse21I CCTNAGG 1 cut(s) 324
Bse3DI GCAATG 1 cut(s) 1203
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 1 cut(s) 1021
BseDI CCNNGG 6 cut(s) 55, 372, 380, 490, 717, 1020
BseGI GGATG 2 cut(s) 696, 1048
BseJI GATNNNNATC 1 cut(s) 12
BseMI GCAATG 1 cut(s) 1203
BseMII CTCAG 4 cut(s) 531, 1082, 1091, 1190
BseNI ACTGG 2 cut(s) 557, 835
BseRI GAGGAG 2 cut(s) 65, 175
BseSI GKGCMC 2 cut(s) 489, 1142
BseXI GCAGC 1 cut(s) 1182
BsgI GTGCAG 1 cut(s) 779
Bsh1236I CGCG 2 cut(s) 633, 1064
BshFI GGCC 5 cut(s) 54, 60, 377, 570, 795
BshNI GGYRCC 1 cut(s) 486
BsiHKAI GWGCWC 1 cut(s) 1070
BsiSI CCGG 1 cut(s) 1165
BslFI GGGAC 1 cut(s) 456
BsmAI GTCTC 2 cut(s) 546, 867
BsmBI CGTCTC 1 cut(s) 546
BsmFI GGGAC 1 cut(s) 456
BsmI GAATGC 1 cut(s) 649
BsnI GGCC 5 cut(s) 54, 60, 377, 570, 795
Bso31I GGTCTC 1 cut(s) 867
Bsp1286I GDGCHC 3 cut(s) 489, 1070, 1142
Bsp143I GATC 5 cut(s) 7, 520, 772, 848, 898
Bsp1720I GCTNAGC 1 cut(s) 1199
Bsp19I CCATGG 1 cut(s) 372
BspACI CCGC 4 cut(s) 320, 631, 780, 1041
BspANI GGCC 5 cut(s) 54, 60, 377, 570, 795
BspCNI CTCAG 4 cut(s) 530, 1083, 1092, 1191
BspFNI CGCG 2 cut(s) 633, 1064
BspLI GGNNCC 4 cut(s) 378, 444, 459, 488
BspMI ACCTGC 1 cut(s) 751
BspPI GGATC 4 cut(s) 15, 515, 767, 856
BspQI GCTCTTC 1 cut(s) 654
BspT107I GGYRCC 1 cut(s) 486
BspTNI GGTCTC 1 cut(s) 867
BsrDI GCAATG 1 cut(s) 1203
BsrI ACTGG 2 cut(s) 557, 835
BssECI CCNNGG 6 cut(s) 55, 372, 380, 490, 717, 1020
BssMI GATC 5 cut(s) 7, 520, 772, 848, 898
BssT1I CCWWGG 3 cut(s) 55, 372, 490
Bst2UI CCWGG 1 cut(s) 1021
Bst4CI ACNGT 5 cut(s) 262, 721, 1015, 1081, 1215
Bst6I CTCTTC 5 cut(s) 136, 154, 193, 654, 710
BstAPI GCANNNNNTGC 1 cut(s) 964
BstC8I GCNNGC 1 cut(s) 1066
BstDEI CTNAG 5 cut(s) 324, 517, 1091, 1100, 1199
BstDSI CCRYGG 2 cut(s) 372, 717
BstF5I GGATG 2 cut(s) 696, 1048
BstFNI CGCG 2 cut(s) 633, 1064
BstKTI GATC 5 cut(s) 10, 523, 775, 851, 901
BstMAI GTCTC 2 cut(s) 546, 867
BstMBI GATC 5 cut(s) 7, 520, 772, 848, 898
BstMWI GCNNNNNNNGC 2 cut(s) 374, 964
BstNI CCWGG 1 cut(s) 1021
BstSCI CCNGG 2 cut(s) 1019, 1163
BstSLI GKGCMC 2 cut(s) 489, 1142
BstUI CGCG 2 cut(s) 633, 1064
BstV1I GCAGC 1 cut(s) 1182
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
Bsu36I CCTNAGG 1 cut(s) 324
BsuI GTATCC 1 cut(s) 378
BsuRI GGCC 5 cut(s) 54, 60, 377, 570, 795
BtgI CCRYGG 2 cut(s) 372, 717
BtsCI GGATG 2 cut(s) 696, 1048
BtsI GCAGTG 1 cut(s) 860
BtsIMutI CAGTG 2 cut(s) 842, 860
BveI ACCTGC 1 cut(s) 751
Cac8I GCNNGC 1 cut(s) 1066
Cfr13I GGNCC 4 cut(s) 376, 443, 832, 1043
Csp6I GTAC 4 cut(s) 122, 1060, 1095, 1249
CviAII CATG 7 cut(s) 277, 340, 373, 427, 566, 613, 693
CviQI GTAC 4 cut(s) 122, 1060, 1095, 1249
DdeI CTNAG 5 cut(s) 324, 517, 1091, 1100, 1199
DpnI GATC 5 cut(s) 9, 522, 774, 850, 900
DpnII GATC 5 cut(s) 7, 520, 772, 848, 898
DriI GACNNNNNGTC 1 cut(s) 246
EaeI YGGCCR 3 cut(s) 52, 568, 793
Eam1104I CTCTTC 5 cut(s) 136, 154, 193, 654, 710
Eam1105I GACNNNNNGTC 1 cut(s) 246
EarI CTCTTC 5 cut(s) 136, 154, 193, 654, 710
EciI GGCGGA 1 cut(s) 1056
Eco130I CCWWGG 3 cut(s) 55, 372, 490
Eco31I GGTCTC 1 cut(s) 867
Eco47I GGWCC 3 cut(s) 443, 832, 1043
Eco81I CCTNAGG 1 cut(s) 324
EcoO109I RGGNCCY 1 cut(s) 443
EcoRI GAATTC 2 cut(s) 576, 1000
EcoRII CCWGG 1 cut(s) 1019
EcoT14I CCWWGG 3 cut(s) 55, 372, 490
EcoT22I ATGCAT 1 cut(s) 698
ErhI CCWWGG 3 cut(s) 55, 372, 490
Esp3I CGTCTC 1 cut(s) 546
FaeI CATG 7 cut(s) 280, 343, 376, 430, 569, 616, 696
FaqI GGGAC 1 cut(s) 456
FatI CATG 7 cut(s) 276, 339, 372, 426, 565, 612, 692
FauNDI CATATG 1 cut(s) 237
FblI GTMKAC 2 cut(s) 160, 723
Fnu4HI GCNGC 3 cut(s) 320, 631, 1196
FokI GGATG 2 cut(s) 683, 1035
Fsp4HI GCNGC 3 cut(s) 320, 631, 1196
FspBI CTAG 2 cut(s) 1035, 1252
GluI GCNGC 3 cut(s) 320, 631, 1196
GsuI CTGGAG 1 cut(s) 382
HaeIII GGCC 5 cut(s) 54, 60, 377, 570, 795
HapII CCGG 1 cut(s) 1165
Hin1II CATG 7 cut(s) 280, 343, 376, 430, 569, 616, 696
HinfI GANTC 4 cut(s) 117, 146, 536, 689
HpaII CCGG 1 cut(s) 1165
HphI GGTGA 2 cut(s) 82, 536
Hpy166II GTNNAC 4 cut(s) 161, 514, 610, 724
Hpy188III TCNNGA 5 cut(s) 265, 361, 433, 653, 922
Hpy8I GTNNAC 4 cut(s) 161, 514, 610, 724
HpyAV CCTTC 3 cut(s) 33, 1099, 1128
HpyCH4III ACNGT 5 cut(s) 262, 721, 1015, 1081, 1215
HpyCH4IV ACGT 1 cut(s) 670
HpyCH4V TGCA 6 cut(s) 696, 760, 933, 950, 1208, 1222
HpyF10VI GCNNNNNNNGC 2 cut(s) 374, 964
HpyF3I CTNAG 5 cut(s) 324, 517, 1091, 1100, 1199
HpySE526I ACGT 1 cut(s) 670
Hsp92II CATG 7 cut(s) 280, 343, 376, 430, 569, 616, 696
Kzo9I GATC 5 cut(s) 7, 520, 772, 848, 898
LguI GCTCTTC 1 cut(s) 654
LmnI GCTCC 1 cut(s) 463
Lsp1109I GCAGC 1 cut(s) 1182
LweI GCATC 1 cut(s) 705
MaeI CTAG 2 cut(s) 1035, 1252
MaeII ACGT 1 cut(s) 670
MaeIII GTNAC 4 cut(s) 262, 1015, 1075, 1109
MalI GATC 5 cut(s) 9, 522, 774, 850, 900
MboI GATC 5 cut(s) 7, 520, 772, 848, 898
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 3 cut(s) 489, 1070, 1142
MlsI TGGCCA 1 cut(s) 54
MluCI AATT 9 cut(s) 243, 299, 353, 576, 636, 709, 1000, 1180, 1223
MluNI TGGCCA 1 cut(s) 54
MlyI GAGTC 1 cut(s) 140
Mox20I TGGCCA 1 cut(s) 54
Mph1103I ATGCAT 1 cut(s) 698
MscI TGGCCA 1 cut(s) 54
MseI TTAA 2 cut(s) 69, 1217
Msp20I TGGCCA 1 cut(s) 54
MspI CCGG 1 cut(s) 1165
MspR9I CCNGG 2 cut(s) 1021, 1165
Mva1269I GAATGC 1 cut(s) 649
MvaI CCWGG 1 cut(s) 1021
MvnI CGCG 2 cut(s) 633, 1064
MwoI GCNNNNNNNGC 2 cut(s) 374, 964
NciI CCSGG 1 cut(s) 1165
NcoI CCATGG 1 cut(s) 372
NdeI CATATG 1 cut(s) 237
NdeII GATC 5 cut(s) 7, 520, 772, 848, 898
NlaIII CATG 7 cut(s) 280, 343, 376, 430, 569, 616, 696
NlaIV GGNNCC 4 cut(s) 378, 444, 459, 488
NmeAIII GCCGAG 1 cut(s) 649
NmuCI GTSAC 3 cut(s) 262, 1015, 1075
NsiI ATGCAT 1 cut(s) 698
PciSI GCTCTTC 1 cut(s) 654
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PctI GAATGC 1 cut(s) 649
PfeI GAWTC 3 cut(s) 117, 536, 689
PflFI GACNNNGTC 1 cut(s) 260
PkrI GCNGC 3 cut(s) 321, 632, 1197
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PpuMI RGGWCCY 1 cut(s) 443
PsiI TTATAA 1 cut(s) 531
Psp1406I AACGTT 1 cut(s) 670
Psp5II RGGWCCY 1 cut(s) 443
Psp6I CCWGG 1 cut(s) 1019
PspGI CCWGG 1 cut(s) 1019
PspN4I GGNNCC 4 cut(s) 378, 444, 459, 488
PspPI GGNCC 4 cut(s) 376, 443, 832, 1043
PspPPI RGGWCCY 1 cut(s) 443
PsuI RGATCY 1 cut(s) 520
PsyI GACNNNGTC 1 cut(s) 260
RsaI GTAC 4 cut(s) 123, 1061, 1096, 1250
RsaNI GTAC 4 cut(s) 122, 1060, 1095, 1249
SapI GCTCTTC 1 cut(s) 654
SaqAI TTAA 2 cut(s) 69, 1217
SatI GCNGC 3 cut(s) 320, 631, 1196
Sau3AI GATC 5 cut(s) 7, 520, 772, 848, 898
Sau96I GGNCC 4 cut(s) 376, 443, 832, 1043
ScaI AGTACT 2 cut(s) 1096, 1250
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 1021, 1165
SduI GDGCHC 3 cut(s) 489, 1070, 1142
SfaNI GCATC 1 cut(s) 705
SinI GGWCC 3 cut(s) 443, 832, 1043
Sse9I AATT 9 cut(s) 243, 299, 353, 576, 636, 709, 1000, 1180, 1223
SsiI CCGC 4 cut(s) 320, 631, 780, 1041
SspI AATATT 1 cut(s) 229
SspMI CTAG 2 cut(s) 1035, 1252
StyD4I CCNGG 2 cut(s) 1019, 1163
StyI CCWWGG 3 cut(s) 55, 372, 490
TaaI ACNGT 5 cut(s) 262, 721, 1015, 1081, 1215
TaiI ACGT 1 cut(s) 673
TaqI TCGA 2 cut(s) 654, 1056
TaqII GACCGA 1 cut(s) 296
TasI AATT 9 cut(s) 243, 299, 353, 576, 636, 709, 1000, 1180, 1223
TatI WGTACW 2 cut(s) 1094, 1248
TauI GCSGC 2 cut(s) 322, 633
TfiI GAWTC 3 cut(s) 117, 536, 689
Tru1I TTAA 2 cut(s) 69, 1217
Tru9I TTAA 2 cut(s) 69, 1217
TscAI CASTG 2 cut(s) 842, 860
TseFI GTSAC 3 cut(s) 262, 1015, 1075
TseI GCWGC 1 cut(s) 1195
Tsp45I GTSAC 3 cut(s) 262, 1015, 1075
TspDTI ATGAA 9 cut(s) 17, 192, 278, 415, 440, 549, 554, 819, 992
TspGWI ACGGA 3 cut(s) 201, 387, 513
TspRI CASTG 2 cut(s) 842, 860
Tth111I GACNNNGTC 1 cut(s) 260
VpaK11BI GGWCC 3 cut(s) 443, 832, 1043
XapI RAATTY 3 cut(s) 576, 1000, 1180
XmiI GTMKAC 2 cut(s) 160, 723
XspI CTAG 2 cut(s) 1035, 1252
ZrmI AGTACT 2 cut(s) 1096, 1250
Zsp2I ATGCAT 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.